RLG00000017573

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0009888
Physical Location & Seq
Forward (+)
18409749 .. 18412089
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017573

Sequence Viewer

Length: 894 bp
ATGAGAATCGACCCAATAATGAGACCCAAATCCCTGAAAGGGAATTGGAGCTCCAGGTTGAAGTTCAAGGCTAAGCTACGATTACCCACCGTCCTCGTTTTCTGCTCTTTCTTCTTCCTCGCCGGTTTCTTCATCTCCTCTCTTTTCTCTCAGGATATAGATGGTGGTGGCAATACACCCAGAACGAGGTTGCTGGAAACAGAGTACGAAACGCTGCCGTTTGGGGAGACAGGAGACGAGTCTTTAACTTCAATTCCCTTCCAGGTGTTGAGCTGGTATCCACGAGCATTATATTTTCCTAATTTTGCTTCTGCGGAGCAATGTGAGACTATAATCGATTTGGCAAAGCCGGACCTTAAACCCTCGACCTTGGCATTGAGAGAGGGAGAGACTGAGGAGAACACCAAGGGAATTAGAACAAGTTCTGGGATGTTTCTTTCGGCTCATCAGGACAAGTCTGGGACTTTGGATGTCATTGAGGAAAAGATTGCAAGAGCAACAATGATTCCCAGGACCCATGGAGAGGCATTCAACGTCTTGCGTTATGAGATTGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCGGAGGAATATGGTCCACAGACAAGCCAAAGGGTTGCATCATTCCTGTTGTATTTAACTGATGTTGAAGAAGGTGGGGAAACAATGTTTCCATACGAGAGTGGCTTGAACCTGGATGGAAAACATGATTCTCAGGAATGTATGGGTTTGAGAGTGAAGCCGCGCAGAGGAGATGGCCTTCTGTTTTATTCATTGTTCCCTAACGGTTCCATTGATCCGTTATCAATACATGGGAGTTGTCCTGTAATCAAAGGCGAAAAATGGGTGGCTACAAAGTGGATGAGGGATCAAGTACAAGAGGAATGA

Protein Analysis

298

Amino Acids

33.59

Weight (kDa)

5.52

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 178 - 291 2.4e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 751
AciI CCGC 2 cut(s) 314, 749
AclWI GGATC 2 cut(s) 797, 882
AfaI GTAC 2 cut(s) 206, 882
AfiI CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
AgsI TTSAA 6 cut(s) 61, 67, 252, 532, 656, 697
AjnI CCWGG 4 cut(s) 53, 261, 509, 699
AluBI AGCT 3 cut(s) 51, 76, 273
AluI AGCT 3 cut(s) 51, 76, 273
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 5 cut(s) 16, 221, 228, 320, 383
AlwI GGATC 2 cut(s) 797, 882
AoxI GGCC 1 cut(s) 763
ApeKI GCWGC 1 cut(s) 214
Asp700I GAANNNNTTC 1 cut(s) 421
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 3 cut(s) 352, 513, 602
AsuC2I CCSGG 1 cut(s) 590
AvaII GGWCC 3 cut(s) 352, 513, 602
BanII GRGCYC 1 cut(s) 53
BauI CACGAG 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 201
BccI CCATC 3 cut(s) 155, 698, 755
BceAI ACGGC 1 cut(s) 202
BciT130I CCWGG 4 cut(s) 55, 263, 511, 701
BciVI GTATCC 1 cut(s) 288
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 5 cut(s) 16, 221, 228, 320, 383
BfuI GTATCC 1 cut(s) 288
BisI GCNGC 2 cut(s) 215, 749
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 2 cut(s) 216, 750
Bme1390I CCNGG 5 cut(s) 55, 263, 511, 590, 701
Bme18I GGWCC 3 cut(s) 352, 513, 602
BmgT120I GGNCC 3 cut(s) 352, 513, 602
BmiI GGNNCC 2 cut(s) 515, 796
BmrFI CCNGG 5 cut(s) 55, 263, 511, 590, 701
BmsI GCATC 2 cut(s) 568, 635
BpmI CTGGAG 1 cut(s) 37
Bpu1102I GCTNAGC 1 cut(s) 72
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 4 cut(s) 369, 405, 509, 517
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
Bse118I RCCGGY 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 326
BseBI CCWGG 4 cut(s) 55, 263, 511, 701
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 369, 405, 509, 517
BseGI GGATG 4 cut(s) 435, 475, 709, 873
BseLI CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 3 cut(s) 164, 384, 734
BseRI GAGGAG 3 cut(s) 127, 410, 771
BseXI GCAGC 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 751
BshFI GGCC 1 cut(s) 765
BshVI ATCGAT 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 3 cut(s) 123, 350, 590
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
BsmAI GTCTC 5 cut(s) 16, 221, 228, 320, 383
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 2 cut(s) 527, 581
BsnI GGCC 1 cut(s) 765
Bso31I GGTCTC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 2 cut(s) 802, 874
Bsp1720I GCTNAGC 1 cut(s) 72
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 2 cut(s) 314, 749
BspANI GGCC 1 cut(s) 765
BspCNI CTCAG 3 cut(s) 163, 385, 733
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 1 cut(s) 751
BspLI GGNNCC 2 cut(s) 515, 796
BspPI GGATC 2 cut(s) 797, 882
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 369, 405, 509, 517
BssMI GATC 2 cut(s) 802, 874
BssSI CACGAG 1 cut(s) 282
BssT1I CCWWGG 3 cut(s) 369, 405, 517
Bst2BI CACGAG 1 cut(s) 282
Bst2UI CCWGG 4 cut(s) 55, 263, 511, 701
Bst4CI ACNGT 2 cut(s) 91, 794
BstDEI CTNAG 4 cut(s) 72, 150, 393, 720
BstDSI CCRYGG 1 cut(s) 517
BstF5I GGATG 4 cut(s) 435, 475, 709, 873
BstFNI CGCG 1 cut(s) 751
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 2 cut(s) 805, 877
BstMAI GTCTC 5 cut(s) 16, 221, 228, 320, 383
BstMBI GATC 2 cut(s) 802, 874
BstNI CCWGG 4 cut(s) 55, 263, 511, 701
BstSCI CCNGG 5 cut(s) 53, 261, 509, 588, 699
BstUI CGCG 1 cut(s) 751
BstV1I GCAGC 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 336
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 1 cut(s) 765
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 517
BtsCI GGATG 4 cut(s) 435, 475, 709, 873
CfoI GCGC 1 cut(s) 753
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 352, 513, 602
ClaI ATCGAT 1 cut(s) 336
Csp6I GTAC 2 cut(s) 205, 881
CviAII CATG 3 cut(s) 518, 713, 818
CviQI GTAC 2 cut(s) 205, 881
DdeI CTNAG 4 cut(s) 72, 150, 393, 720
DpnI GATC 2 cut(s) 804, 876
DpnII GATC 2 cut(s) 802, 874
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 3 cut(s) 369, 405, 517
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 3 cut(s) 352, 513, 602
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 513
EcoRII CCWGG 4 cut(s) 53, 261, 509, 699
EcoT14I CCWWGG 3 cut(s) 369, 405, 517
EcoT22I ATGCAT 1 cut(s) 583
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 3 cut(s) 369, 405, 517
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 3 cut(s) 521, 716, 821
FaqI GGGAC 1 cut(s) 475
FatI CATG 3 cut(s) 517, 712, 817
Fnu4HI GCNGC 2 cut(s) 215, 749
FokI GGATG 4 cut(s) 442, 482, 716, 880
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 215, 749
GlaI GCGC 1 cut(s) 752
GluI GCNGC 2 cut(s) 215, 749
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 1 cut(s) 765
HapII CCGG 3 cut(s) 123, 350, 590
HhaI GCGC 1 cut(s) 753
Hin1II CATG 3 cut(s) 521, 716, 821
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 4 cut(s) 6, 239, 505, 716
HpaII CCGG 3 cut(s) 123, 350, 590
Hpy166II GTNNAC 1 cut(s) 605
Hpy188III TCNNGA 3 cut(s) 152, 449, 722
Hpy8I GTNNAC 1 cut(s) 605
HpyAV CCTTC 3 cut(s) 268, 653, 776
HpyCH4III ACNGT 2 cut(s) 91, 794
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 3 cut(s) 491, 581, 626
HpyF3I CTNAG 4 cut(s) 72, 150, 393, 720
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 3 cut(s) 521, 716, 821
HspAI GCGC 1 cut(s) 751
Kzo9I GATC 2 cut(s) 802, 874
LmnI GCTCC 3 cut(s) 48, 56, 316
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 2 cut(s) 568, 635
MaeII ACGT 1 cut(s) 534
MalI GATC 2 cut(s) 804, 876
MboI GATC 2 cut(s) 802, 874
MboII GAAGA 4 cut(s) 103, 106, 121, 668
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 5 cut(s) 43, 252, 301, 411, 565
MlyI GAGTC 1 cut(s) 248
Mph1103I ATGCAT 1 cut(s) 583
MroXI GAANNNNTTC 1 cut(s) 421
MseI TTAA 3 cut(s) 245, 357, 644
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 3 cut(s) 123, 350, 590
MspR9I CCNGG 5 cut(s) 55, 263, 511, 590, 701
Mva1269I GAATGC 2 cut(s) 527, 581
MvaI CCWGG 4 cut(s) 55, 263, 511, 701
MvnI CGCG 1 cut(s) 751
NciI CCSGG 1 cut(s) 590
NcoI CCATGG 1 cut(s) 517
NdeII GATC 2 cut(s) 802, 874
NlaIII CATG 3 cut(s) 521, 716, 821
NlaIV GGNNCC 2 cut(s) 515, 796
NsiI ATGCAT 1 cut(s) 583
PctI GAATGC 2 cut(s) 527, 581
PdmI GAANNNNTTC 1 cut(s) 421
PfeI GAWTC 3 cut(s) 6, 505, 716
PkrI GCNGC 2 cut(s) 216, 750
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 53
Psp5II RGGWCCY 1 cut(s) 513
Psp6I CCWGG 4 cut(s) 53, 261, 509, 699
PspGI CCWGG 4 cut(s) 53, 261, 509, 699
PspN4I GGNNCC 2 cut(s) 515, 796
PspPI GGNCC 3 cut(s) 352, 513, 602
PspPPI RGGWCCY 1 cut(s) 513
RsaI GTAC 2 cut(s) 206, 882
RsaNI GTAC 2 cut(s) 205, 881
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 3 cut(s) 245, 357, 644
SatI GCNGC 2 cut(s) 215, 749
Sau3AI GATC 2 cut(s) 802, 874
Sau96I GGNCC 3 cut(s) 352, 513, 602
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 5 cut(s) 55, 263, 511, 590, 701
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 2 cut(s) 568, 635
SinI GGWCC 3 cut(s) 352, 513, 602
SmiMI CAYNNNNRTG 1 cut(s) 576
Sse9I AATT 5 cut(s) 43, 252, 301, 411, 565
SsiI CCGC 2 cut(s) 314, 749
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 5 cut(s) 53, 261, 509, 588, 699
StyI CCWWGG 3 cut(s) 369, 405, 517
TaaI ACNGT 2 cut(s) 91, 794
TaiI ACGT 1 cut(s) 537
TaqI TCGA 3 cut(s) 9, 336, 365
TasI AATT 5 cut(s) 43, 252, 301, 411, 565
TatI WGTACW 1 cut(s) 880
TauI GCSGC 1 cut(s) 751
TfiI GAWTC 3 cut(s) 6, 505, 716
Tru1I TTAA 3 cut(s) 245, 357, 644
Tru9I TTAA 3 cut(s) 245, 357, 644
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 121, 768
TspGWI ACGGA 1 cut(s) 795
VpaK11BI GGWCC 3 cut(s) 352, 513, 602
XmnI GAANNNNTTC 1 cut(s) 421
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.