Rh2AG193100

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009888
Physical Location & Seq
Forward (+)
19132929 .. 19135283
2355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG193100.1

Sequence Viewer

Length: 894 bp
ATGAGAATCGACCCAATAATGAGACCCAAATCCCTGAAAGGGAATTGGAGCTCCAGGTTGAAGTTCAAGGCCAAGCTACGATTACCCACCGTCCTCGTTTTCTGCTCTTTCTTCTTCCTCGTCGGTTTCTTCATCTCCTCTCTTTTCTCTCAGGATGTAGATGGTGGTGGCAATACACCCAGAACGAGGTTGCTGGAAACAGAGTACGAAACGCTGCCGTTTGGGGAGACAGGAGACGAGTCTTTAACTTCAATTCCCTTCCAGGTGTTGAGCTGGTATCCGCGAGCATTATATTTTCCTAATTTTGCTTCTGCGGAGCAATGTGAGACTATAATCGATTTGGCAAAGCCGGCCCTTAAACCCTCGACCTTGGCTTTGAGAGAGGGAGAGACTGAGGAGAACACAAAGGGAATTAGAACAAGTTCTGGGATGTTTCTTTCGGCTTATCAAGACAAGTCTGGGACTTTGGATGTCATTGAGGAAAAGATTGCAAGAGCAACAATGATTCCCAGGACCCATGGAGAGGCATTCAACGTCTTGTGTTATGAGATTGGGCAGAAGTATAATTCTCATTATGATGCATTCCACCCGGAGGAATATGGTCCACAGACAAGCCAAAGGGTTGCATCATTCCTGTTGTATTTAACTGATGTTGAAGAAGGCGGGGAAACAATGTTTCCATACGAGAGTGGCTTGAACCTGGATGGAAAACATGATTCTCAGGAATGTATGGGTTTGAGAGTGAAGCCGCGCAGAGGAGATGGCCTTCTGTTTTATTCATTGTTCCCTAACGGTTCCATTGACCCTTTATCAATACATGGGAGTTGTCCTGTAATCAAAGGCGAAAAATGGGTGGCTACAAAGTGGATGAGGGATCAAGTACAAGAGGAATGA

Protein Analysis

297

Amino Acids

33.53

Weight (kDa)

5.43

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_3 PF13640 178 - 291 3.8e-16 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 283, 751
AciI CCGC 4 cut(s) 281, 314, 663, 749
AclWI GGATC 1 cut(s) 882
AfaI GTAC 2 cut(s) 206, 882
AfiI CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
AgsI TTSAA 6 cut(s) 61, 67, 252, 532, 656, 697
AjnI CCWGG 4 cut(s) 53, 261, 509, 699
AluBI AGCT 3 cut(s) 51, 76, 273
AluI AGCT 3 cut(s) 51, 76, 273
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 5 cut(s) 16, 221, 228, 320, 383
AlwI GGATC 1 cut(s) 882
AoxI GGCC 3 cut(s) 69, 351, 763
ApeKI GCWGC 1 cut(s) 214
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
Asp700I GAANNNNTTC 1 cut(s) 421
AspLEI GCGC 1 cut(s) 753
AspS9I GGNCC 3 cut(s) 352, 513, 602
AsuC2I CCSGG 1 cut(s) 590
AvaII GGWCC 2 cut(s) 513, 602
BanII GRGCYC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 201
BccI CCATC 3 cut(s) 155, 698, 755
BceAI ACGGC 1 cut(s) 202
BciT130I CCWGG 4 cut(s) 55, 263, 511, 701
BciVI GTATCC 1 cut(s) 288
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 5 cut(s) 16, 221, 228, 320, 383
BfuI GTATCC 1 cut(s) 288
BisI GCNGC 2 cut(s) 215, 749
BlsI GCNGC 2 cut(s) 216, 750
Bme1390I CCNGG 5 cut(s) 55, 263, 511, 590, 701
Bme18I GGWCC 2 cut(s) 513, 602
BmgT120I GGNCC 3 cut(s) 352, 513, 602
BmiI GGNNCC 2 cut(s) 515, 796
BmrFI CCNGG 5 cut(s) 55, 263, 511, 590, 701
BmsI GCATC 2 cut(s) 568, 635
BpmI CTGGAG 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 336
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 3 cut(s) 369, 509, 517
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
Bse118I RCCGGY 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 326
BseBI CCWGG 4 cut(s) 55, 263, 511, 701
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 369, 509, 517
BseGI GGATG 5 cut(s) 160, 435, 475, 709, 873
BseLI CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
BseMI GCAATG 1 cut(s) 326
BseMII CTCAG 3 cut(s) 164, 384, 734
BseRI GAGGAG 3 cut(s) 127, 410, 771
BseXI GCAGC 1 cut(s) 201
Bsh1236I CGCG 2 cut(s) 283, 751
BshFI GGCC 3 cut(s) 71, 353, 765
BshVI ATCGAT 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 53
BsiSI CCGG 2 cut(s) 350, 590
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 5 cut(s) 39, 186, 523, 592, 755
BsmAI GTCTC 5 cut(s) 16, 221, 228, 320, 383
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 475
BsmI GAATGC 2 cut(s) 527, 581
BsnI GGCC 3 cut(s) 71, 353, 765
Bso31I GGTCTC 1 cut(s) 16
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 874
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 4 cut(s) 281, 314, 663, 749
BspANI GGCC 3 cut(s) 71, 353, 765
BspCNI CTCAG 3 cut(s) 163, 385, 733
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 2 cut(s) 283, 751
BspLI GGNNCC 2 cut(s) 515, 796
BspPI GGATC 1 cut(s) 882
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 326
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 3 cut(s) 369, 509, 517
BssMI GATC 1 cut(s) 874
BssT1I CCWWGG 2 cut(s) 369, 517
Bst2UI CCWGG 4 cut(s) 55, 263, 511, 701
Bst4CI ACNGT 2 cut(s) 91, 794
BstC8I GCNNGC 2 cut(s) 285, 351
BstDEI CTNAG 3 cut(s) 150, 393, 720
BstDSI CCRYGG 1 cut(s) 517
BstF5I GGATG 5 cut(s) 160, 435, 475, 709, 873
BstFNI CGCG 2 cut(s) 283, 751
BstHHI GCGC 1 cut(s) 753
BstKTI GATC 1 cut(s) 877
BstMAI GTCTC 5 cut(s) 16, 221, 228, 320, 383
BstMBI GATC 1 cut(s) 874
BstMWI GCNNNNNNNGC 1 cut(s) 350
BstNI CCWGG 4 cut(s) 55, 263, 511, 701
BstSCI CCNGG 5 cut(s) 53, 261, 509, 588, 699
BstUI CGCG 2 cut(s) 283, 751
BstV1I GCAGC 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 336
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 3 cut(s) 71, 353, 765
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 517
BtsCI GGATG 5 cut(s) 160, 435, 475, 709, 873
Cac8I GCNNGC 2 cut(s) 285, 351
CfoI GCGC 1 cut(s) 753
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 3 cut(s) 352, 513, 602
ClaI ATCGAT 1 cut(s) 336
Csp6I GTAC 2 cut(s) 205, 881
CviAII CATG 3 cut(s) 518, 713, 818
CviQI GTAC 2 cut(s) 205, 881
DdeI CTNAG 3 cut(s) 150, 393, 720
DpnI GATC 1 cut(s) 876
DpnII GATC 1 cut(s) 874
Ecl136II GAGCTC 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 369, 517
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 16
Eco47I GGWCC 2 cut(s) 513, 602
Eco53kI GAGCTC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 513
EcoRII CCWGG 4 cut(s) 53, 261, 509, 699
EcoT14I CCWWGG 2 cut(s) 369, 517
EcoT22I ATGCAT 1 cut(s) 583
EcoT38I GRGCYC 1 cut(s) 53
ErhI CCWWGG 2 cut(s) 369, 517
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 3 cut(s) 521, 716, 821
FaqI GGGAC 1 cut(s) 475
FatI CATG 3 cut(s) 517, 712, 817
FauI CCCGC 1 cut(s) 656
Fnu4HI GCNGC 2 cut(s) 215, 749
FokI GGATG 5 cut(s) 167, 442, 482, 716, 880
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 2 cut(s) 215, 749
GlaI GCGC 1 cut(s) 752
GluI GCNGC 2 cut(s) 215, 749
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 3 cut(s) 71, 353, 765
HapII CCGG 2 cut(s) 350, 590
HhaI GCGC 1 cut(s) 753
Hin1II CATG 3 cut(s) 521, 716, 821
Hin6I GCGC 1 cut(s) 751
HinP1I GCGC 1 cut(s) 751
HinfI GANTC 4 cut(s) 6, 239, 505, 716
HpaII CCGG 2 cut(s) 350, 590
Hpy166II GTNNAC 1 cut(s) 605
Hpy188III TCNNGA 3 cut(s) 152, 449, 722
Hpy8I GTNNAC 1 cut(s) 605
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 268, 653, 776
HpyCH4III ACNGT 2 cut(s) 91, 794
HpyCH4IV ACGT 1 cut(s) 534
HpyCH4V TGCA 3 cut(s) 491, 581, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 350
HpyF3I CTNAG 3 cut(s) 150, 393, 720
HpySE526I ACGT 1 cut(s) 534
Hsp92II CATG 3 cut(s) 521, 716, 821
HspAI GCGC 1 cut(s) 751
KroI GCCGGC 1 cut(s) 349
KroNI GCCGGC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 874
LmnI GCTCC 3 cut(s) 48, 56, 316
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 2 cut(s) 568, 635
MaeII ACGT 1 cut(s) 534
MalI GATC 1 cut(s) 876
MboI GATC 1 cut(s) 874
MboII GAAGA 4 cut(s) 103, 106, 121, 668
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 5 cut(s) 43, 252, 301, 411, 565
MlyI GAGTC 1 cut(s) 248
Mph1103I ATGCAT 1 cut(s) 583
MroNI GCCGGC 1 cut(s) 349
MroXI GAANNNNTTC 1 cut(s) 421
MseI TTAA 3 cut(s) 245, 357, 644
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 2 cut(s) 350, 590
MspR9I CCNGG 5 cut(s) 55, 263, 511, 590, 701
Mva1269I GAATGC 2 cut(s) 527, 581
MvaI CCWGG 4 cut(s) 55, 263, 511, 701
MvnI CGCG 2 cut(s) 283, 751
MwoI GCNNNNNNNGC 1 cut(s) 350
NaeI GCCGGC 1 cut(s) 351
NciI CCSGG 1 cut(s) 590
NcoI CCATGG 1 cut(s) 517
NdeII GATC 1 cut(s) 874
NgoMIV GCCGGC 1 cut(s) 349
NlaIII CATG 3 cut(s) 521, 716, 821
NlaIV GGNNCC 2 cut(s) 515, 796
NsiI ATGCAT 1 cut(s) 583
PctI GAATGC 2 cut(s) 527, 581
PdiI GCCGGC 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 421
PfeI GAWTC 3 cut(s) 6, 505, 716
PkrI GCNGC 2 cut(s) 216, 750
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PpuMI RGGWCCY 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 53
Psp5II RGGWCCY 1 cut(s) 513
Psp6I CCWGG 4 cut(s) 53, 261, 509, 699
PspGI CCWGG 4 cut(s) 53, 261, 509, 699
PspN4I GGNNCC 2 cut(s) 515, 796
PspPI GGNCC 3 cut(s) 352, 513, 602
PspPPI RGGWCCY 1 cut(s) 513
RsaI GTAC 2 cut(s) 206, 882
RsaNI GTAC 2 cut(s) 205, 881
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 53
SaqAI TTAA 3 cut(s) 245, 357, 644
SatI GCNGC 2 cut(s) 215, 749
Sau3AI GATC 1 cut(s) 874
Sau96I GGNCC 3 cut(s) 352, 513, 602
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 5 cut(s) 55, 263, 511, 590, 701
SduI GDGCHC 1 cut(s) 53
SetI ASST 9 cut(s) 53, 59, 78, 191, 267, 275, 371, 537, 702
SfaNI GCATC 2 cut(s) 568, 635
SinI GGWCC 2 cut(s) 513, 602
SmiMI CAYNNNNRTG 1 cut(s) 576
Sse9I AATT 5 cut(s) 43, 252, 301, 411, 565
SsiI CCGC 4 cut(s) 281, 314, 663, 749
SstI GAGCTC 1 cut(s) 53
StyD4I CCNGG 5 cut(s) 53, 261, 509, 588, 699
StyI CCWWGG 2 cut(s) 369, 517
TaaI ACNGT 2 cut(s) 91, 794
TaiI ACGT 1 cut(s) 537
TaqI TCGA 3 cut(s) 9, 336, 365
TasI AATT 5 cut(s) 43, 252, 301, 411, 565
TatI WGTACW 1 cut(s) 880
TauI GCSGC 1 cut(s) 751
TfiI GAWTC 3 cut(s) 6, 505, 716
Tru1I TTAA 3 cut(s) 245, 357, 644
Tru9I TTAA 3 cut(s) 245, 357, 644
TseI GCWGC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 121, 768
VpaK11BI GGWCC 2 cut(s) 513, 602
XmnI GAANNNNTTC 1 cut(s) 421
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.