MD02G1287000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009711
Physical Location & Seq
Reverse (-)
34377141 .. 34381972
4832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1287000.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGACTTGGTTGGGTGCTTCCTCTTCTTCTTCCCCATGGATTCTCAGGAACTCGCTTCTCACAACCTCTTCATCTTCTTCTTCCTCTTCAAAATTCTCGAAATACCAATCATCCCCTCGCCCTCTTCGCCTCTCGTGCCGCTCCCAATCCGACGTCGGCAGTGACTTCAATTTCGTGCTCCACGATGCTCTCGATTCTTCCGGAACCCTCACCTCCCTCGCCAGAGAGGCCAGGGAGGGGTTCTGCTCACAAATTCGCCATCTGTCTGATATAGAGAGGGAAACCAGCATTACCATCAATAGATGTGTTGATTTGGGGAAAACAGCTCTTTACATTGCAGCAGAAGATGACTCCCTTGTGTCACATTCTTCAGTTCCGCTTCCGGTGGATGCTTTTATTAGTAGGTTTGGTGATCTTTCCATGGACTATTGTTCTCACTACAACTCTTCATTTAGATCCTCGCCTGAGAAATTGTTGGAGAGTTTGGAAAACTATTTGTACGTAAATAAGGGTTTTCGAAGGACAATTGCAAGGTATCACTCTGAATCACGGGCTCTATATATCCACTCGGTTTTGACACATCGTTCAGGATCACCTGCAATGCTTTCACTCATATACTCAGAAATTTTGAAAATGCTTCGCCTATGGGGTTTTTTGGATTTCGATGTGGAGATATTCTTTCCTCATGATCTTCATAGTCTTCCCAGAGGTTATCATAAACAGAAAAGCAAGGAATCTGATAATGTGCACATAATGACTTCGCAAGCCCTTTTAGTGGAGATTTTAAGAAATTTGAAGGACGCATTCTGGCCGTTTCAGCATGATCAACCAGGGAATTTGTTCTTGAGGGCAGCACGTGCTGCTAACTTTATTGATAGATCAAACATAAATGAAGACAGTGGCTTTCAGCTTGCATCTGCGAAGGCTGCTCAACATAGACTAGATCGTGGTGTTTGGACTAGTGTACATTTTGGGGATATGAGGCGTGCCCTATCTGCTTGTGAACGTCTTATTCTCCTAGAAGCAGATCCGAAGGAATTAAGAGATTACAGCATTCTTCTCTACCATTGTGGATTTTATGAGGAATCACTTGAATATCTAAAGTTGTATCAGGACAAAAAGGGTCGTCCACTACGGAGGCGACCGTCTGATTCGTTAACCGTTTTGGAGGAAGATGCCGCAGAGAAATTGATGATACGCCTTAACCTAATTCTTATGGAGGATGGTTGGAGCAGGCCGTCATATATTAGAAACTTTCTTGGTAACAACTCTGAACCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transglut_core2

Protein Analysis

428

Amino Acids

48.8

Weight (kDa)

6.95

Isoelectric Point (pI)

62.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transglut_core2 PF13369 153 - 214 9.5e-09 Transglutaminase-like superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
AatII GACGTC 1 cut(s) 156
Acc36I ACCTGC 1 cut(s) 604
AccBSI CCGCTC 1 cut(s) 141
AccIII TCCGGA 1 cut(s) 200
AciI CCGC 3 cut(s) 139, 377, 1179
AclWI GGATC 3 cut(s) 450, 598, 1022
AcoI YGGCCR 1 cut(s) 809
AcsI RAATTY 5 cut(s) 92, 252, 624, 790, 835
AcuI CTGAAG 1 cut(s) 354
AcvI CACGTG 1 cut(s) 857
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 2 cut(s) 500, 966
AfiI CCNNNNNNNGG 2 cut(s) 237, 775
AgsI TTSAA 5 cut(s) 90, 169, 631, 796, 1094
AhlI ACTAGT 1 cut(s) 959
AjnI CCWGG 2 cut(s) 230, 829
AluBI AGCT 2 cut(s) 326, 910
AluI AGCT 2 cut(s) 326, 910
Alw21I GWGCWC 2 cut(s) 180, 750
Alw44I GTGCAC 1 cut(s) 746
AlwI GGATC 3 cut(s) 450, 598, 1022
Aor13HI TCCGGA 1 cut(s) 200
AoxI GGCC 3 cut(s) 228, 809, 1235
ApaLI GTGCAC 1 cut(s) 746
ApeKI GCWGC 4 cut(s) 338, 851, 860, 926
ApoI RAATTY 5 cut(s) 92, 252, 624, 790, 835
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
Asp700I GAANNNNTTC 1 cut(s) 839
AsuHPI GGTGA 3 cut(s) 202, 422, 585
AsuII TTCGAA 1 cut(s) 517
BaeGI GKGCMC 2 cut(s) 750, 991
BanII GRGCYC 1 cut(s) 556
BauI CACGAG 1 cut(s) 133
BbrPI CACGTG 1 cut(s) 857
BbsI GAAGAC 2 cut(s) 692, 900
Bbv12I GWGCWC 2 cut(s) 180, 750
BbvI GCAGC 4 cut(s) 350, 847, 863, 913
BccI CCATC 3 cut(s) 267, 302, 1217
BceAI ACGGC 2 cut(s) 796, 1222
BciT130I CCWGG 2 cut(s) 232, 831
BclI TGATCA 1 cut(s) 823
BcuI ACTAGT 1 cut(s) 959
BfaI CTAG 3 cut(s) 941, 960, 1019
BfuAI ACCTGC 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 6 cut(s) 139, 339, 852, 861, 927, 1179
BlsI GCNGC 6 cut(s) 140, 340, 853, 862, 928, 1180
Bme1390I CCNGG 2 cut(s) 232, 831
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 2 cut(s) 232, 831
BmsI GCATC 4 cut(s) 175, 379, 923, 1165
BpiI GAAGAC 2 cut(s) 692, 900
Bpu14I TTCGAA 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 865
BsaAI YACGTR 2 cut(s) 502, 857
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 5 cut(s) 35, 231, 420, 830, 1277
BsaWI WCCGGW 2 cut(s) 200, 382
Bsc4I CCNNNNNNNGG 2 cut(s) 237, 775
Bse3DI GCAATG 2 cut(s) 333, 606
BseAI TCCGGA 1 cut(s) 200
BseBI CCWGG 2 cut(s) 232, 831
BseDI CCNNGG 5 cut(s) 35, 231, 420, 830, 1277
BseGI GGATG 3 cut(s) 110, 394, 1228
BseLI CCNNNNNNNGG 2 cut(s) 237, 775
BseMI GCAATG 2 cut(s) 333, 606
BseMII CTCAG 3 cut(s) 58, 456, 633
BseSI GKGCMC 2 cut(s) 750, 991
BseXI GCAGC 4 cut(s) 350, 847, 863, 913
Bsh1285I CGRYCG 1 cut(s) 1145
BshFI GGCC 3 cut(s) 230, 811, 1237
BsiEI CGRYCG 1 cut(s) 1145
BsiHKAI GWGCWC 2 cut(s) 180, 750
BsiSI CCGG 2 cut(s) 201, 383
BslI CCNNNNNNNGG 2 cut(s) 237, 775
BsmI GAATGC 2 cut(s) 803, 1053
BsnI GGCC 3 cut(s) 230, 811, 1237
Bsp119I TTCGAA 1 cut(s) 517
Bsp1286I GDGCHC 4 cut(s) 180, 556, 750, 991
Bsp13I TCCGGA 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 964
Bsp143I GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
Bsp19I CCATGG 3 cut(s) 35, 420, 1277
BspACI CCGC 3 cut(s) 139, 377, 1179
BspANI GGCC 3 cut(s) 230, 811, 1237
BspCNI CTCAG 3 cut(s) 57, 457, 632
BspEI TCCGGA 1 cut(s) 200
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 3 cut(s) 450, 598, 1022
BspT104I TTCGAA 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 2 cut(s) 333, 606
BsrGI TGTACA 1 cut(s) 964
BssECI CCNNGG 5 cut(s) 35, 231, 420, 830, 1277
BssMI GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 133
BssT1I CCWWGG 3 cut(s) 35, 420, 1277
Bst2BI CACGAG 1 cut(s) 133
Bst2UI CCWGG 2 cut(s) 232, 831
Bst4CI ACNGT 3 cut(s) 899, 1146, 1162
Bst6I CTCTTC 5 cut(s) 28, 73, 91, 129, 451
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 2 cut(s) 857, 860
BstAUI TGTACA 1 cut(s) 964
BstBAI YACGTR 2 cut(s) 502, 857
BstBI TTCGAA 1 cut(s) 517
BstC8I GCNNGC 4 cut(s) 765, 912, 987, 1235
BstDEI CTNAG 3 cut(s) 44, 465, 619
BstDSI CCRYGG 3 cut(s) 35, 420, 1277
BstF5I GGATG 3 cut(s) 110, 394, 1228
BstKTI GATC 8 cut(s) 415, 458, 593, 691, 826, 881, 946, 1030
BstMBI GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
BstMCI CGRYCG 1 cut(s) 1145
BstMWI GCNNNNNNNGC 8 cut(s) 126, 135, 227, 817, 857, 860, 926, 995
BstNI CCWGG 2 cut(s) 232, 831
BstSCI CCNGG 2 cut(s) 230, 829
BstSLI GKGCMC 2 cut(s) 750, 991
BstSNI TACGTA 1 cut(s) 502
BstV1I GCAGC 4 cut(s) 350, 847, 863, 913
BstV2I GAAGAC 2 cut(s) 692, 900
BstX2I RGATCY 2 cut(s) 455, 1027
BstYI RGATCY 2 cut(s) 455, 1027
BsuRI GGCC 3 cut(s) 230, 811, 1237
BtgI CCRYGG 3 cut(s) 35, 420, 1277
BtsCI GGATG 3 cut(s) 110, 394, 1228
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 166, 904
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 4 cut(s) 765, 912, 987, 1235
CciI TCATGA 1 cut(s) 685
CseI GACGC 1 cut(s) 809
Csp6I GTAC 2 cut(s) 499, 965
CviAII CATG 5 cut(s) 36, 421, 686, 821, 1278
CviJI RGCY 9 cut(s) 230, 326, 554, 767, 811, 903, 910, 926, 1237
CviKI_1 RGCY 9 cut(s) 230, 326, 554, 767, 811, 903, 910, 926, 1237
CviQI GTAC 2 cut(s) 499, 965
DdeI CTNAG 3 cut(s) 44, 465, 619
DpnI GATC 8 cut(s) 414, 457, 592, 690, 825, 880, 945, 1029
DpnII GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
EaeI YGGCCR 1 cut(s) 809
Eam1104I CTCTTC 5 cut(s) 28, 73, 91, 129, 451
EarI CTCTTC 5 cut(s) 28, 73, 91, 129, 451
Eco105I TACGTA 1 cut(s) 502
Eco130I CCWWGG 3 cut(s) 35, 420, 1277
Eco24I GRGCYC 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 354
Eco72I CACGTG 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 230, 829
EcoT14I CCWWGG 3 cut(s) 35, 420, 1277
EcoT38I GRGCYC 1 cut(s) 556
ErhI CCWWGG 3 cut(s) 35, 420, 1277
FaeI CATG 5 cut(s) 39, 424, 689, 824, 1281
FatI CATG 5 cut(s) 35, 420, 685, 820, 1277
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 6 cut(s) 139, 339, 852, 861, 927, 1179
FokI GGATG 3 cut(s) 97, 401, 1235
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 6 cut(s) 139, 339, 852, 861, 927, 1179
FspBI CTAG 3 cut(s) 941, 960, 1019
GluI GCNGC 6 cut(s) 139, 339, 852, 861, 927, 1179
HaeIII GGCC 3 cut(s) 230, 811, 1237
HapII CCGG 2 cut(s) 201, 383
HgaI GACGC 1 cut(s) 809
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 5 cut(s) 39, 424, 689, 824, 1281
HincII GTYRAC 1 cut(s) 1158
HindII GTYRAC 1 cut(s) 1158
HinfI GANTC 7 cut(s) 40, 194, 350, 545, 734, 1085, 1151
HpaI GTTAAC 1 cut(s) 1158
HpaII CCGG 2 cut(s) 201, 383
HphI GGTGA 3 cut(s) 202, 422, 585
Hpy166II GTNNAC 5 cut(s) 748, 965, 1004, 1130, 1158
Hpy188I TCNGA 8 cut(s) 151, 268, 544, 622, 739, 1032, 1150, 1273
Hpy188III TCNNGA 8 cut(s) 46, 97, 191, 201, 588, 686, 844, 1112
Hpy8I GTNNAC 5 cut(s) 748, 965, 1004, 1130, 1158
Hpy99I CGWCG 2 cut(s) 155, 158
HpyAV CCTTC 4 cut(s) 513, 790, 916, 1027
HpyCH4III ACNGT 3 cut(s) 899, 1146, 1162
HpyCH4IV ACGT 4 cut(s) 153, 501, 856, 1006
HpyCH4V TGCA 5 cut(s) 338, 530, 599, 748, 914
HpyF10VI GCNNNNNNNGC 8 cut(s) 126, 135, 227, 817, 857, 860, 926, 995
HpyF3I CTNAG 3 cut(s) 44, 465, 619
HpySE526I ACGT 4 cut(s) 153, 501, 856, 1006
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 5 cut(s) 39, 424, 689, 824, 1281
Kpn2I TCCGGA 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 823
KspAI GTTAAC 1 cut(s) 1158
Kzo9I GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
LmnI GCTCC 3 cut(s) 146, 183, 1230
Lsp1109I GCAGC 4 cut(s) 350, 847, 863, 913
LweI GCATC 4 cut(s) 175, 379, 923, 1165
MaeI CTAG 3 cut(s) 941, 960, 1019
MaeII ACGT 4 cut(s) 153, 501, 856, 1006
MaeIII GTNAC 3 cut(s) 161, 360, 1262
MalI GATC 8 cut(s) 414, 457, 592, 690, 825, 880, 945, 1029
MbiI CCGCTC 1 cut(s) 141
MboI GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 2 cut(s) 455, 1027
MhlI GDGCHC 4 cut(s) 180, 556, 750, 991
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 3 cut(s) 174, 456, 1208
MroI TCCGGA 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 4 cut(s) 785, 1040, 1157, 1203
MspI CCGG 2 cut(s) 201, 383
MspR9I CCNGG 2 cut(s) 232, 831
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 2 cut(s) 803, 1053
MvaI CCWGG 2 cut(s) 232, 831
MwoI GCNNNNNNNGC 8 cut(s) 126, 135, 227, 817, 857, 860, 926, 995
NcoI CCATGG 3 cut(s) 35, 420, 1277
NdeII GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
NlaIII CATG 5 cut(s) 39, 424, 689, 824, 1281
NlaIV GGNNCC 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 161, 360
NspV TTCGAA 1 cut(s) 517
PagI TCATGA 1 cut(s) 685
PaqCI CACCTGC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 189
PctI GAATGC 2 cut(s) 803, 1053
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 6 cut(s) 40, 194, 545, 734, 1085, 1151
PkrI GCNGC 6 cut(s) 140, 340, 853, 862, 928, 1180
PleI GAGTC 1 cut(s) 344
PmaCI CACGTG 1 cut(s) 857
PmlI CACGTG 1 cut(s) 857
PpsI GAGTC 1 cut(s) 344
Ppu21I YACGTR 2 cut(s) 502, 857
Psp6I CCWGG 2 cut(s) 230, 829
PspCI CACGTG 1 cut(s) 857
PspGI CCWGG 2 cut(s) 230, 829
PspN4I GGNNCC 1 cut(s) 205
PsuI RGATCY 2 cut(s) 455, 1027
RsaI GTAC 2 cut(s) 500, 966
RsaNI GTAC 2 cut(s) 499, 965
SaqAI TTAA 4 cut(s) 785, 1040, 1157, 1203
SatI GCNGC 6 cut(s) 139, 339, 852, 861, 927, 1179
Sau3AI GATC 8 cut(s) 412, 455, 590, 688, 823, 878, 943, 1027
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 232, 831
SduI GDGCHC 4 cut(s) 180, 556, 750, 991
SfaNI GCATC 4 cut(s) 175, 379, 923, 1165
SfuI TTCGAA 1 cut(s) 517
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
SnaBI TACGTA 1 cut(s) 502
SpeI ACTAGT 1 cut(s) 959
SsiI CCGC 3 cut(s) 139, 377, 1179
SspMI CTAG 3 cut(s) 941, 960, 1019
StyD4I CCNGG 2 cut(s) 230, 829
StyI CCWWGG 3 cut(s) 35, 420, 1277
TaaI ACNGT 3 cut(s) 899, 1146, 1162
TaiI ACGT 4 cut(s) 156, 504, 859, 1009
TaqI TCGA 4 cut(s) 98, 192, 517, 663
TatI WGTACW 1 cut(s) 964
TauI GCSGC 2 cut(s) 141, 1181
TfiI GAWTC 6 cut(s) 40, 194, 545, 734, 1085, 1151
Tru1I TTAA 4 cut(s) 785, 1040, 1157, 1203
Tru9I TTAA 4 cut(s) 785, 1040, 1157, 1203
TscAI CASTG 2 cut(s) 166, 904
TseFI GTSAC 2 cut(s) 161, 360
TseI GCWGC 4 cut(s) 338, 851, 860, 926
Tsp45I GTSAC 2 cut(s) 161, 360
TspDTI ATGAA 4 cut(s) 60, 438, 683, 906
TspGWI ACGGA 1 cut(s) 1150
TspRI CASTG 2 cut(s) 166, 904
VneI GTGCAC 1 cut(s) 746
XapI RAATTY 5 cut(s) 92, 252, 624, 790, 835
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 3 cut(s) 941, 960, 1019
ZraI GACGTC 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.