Rroxscaffold_4G00325710

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009711
Physical Location & Seq
Reverse (-)
57540192 .. 57547330
7139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325710.1

Sequence Viewer

Length: 1497 bp
ATGAGCAGCTGGGTTGGAGCTTCTTCTTCTTCTTCTCCATGGCCTCTCAGGACCTTTTCCTCTTCCTCCTCCTATCCTTCCAAATTCTTCAAATTCCATTCATGTCCTCCTCCTTCTCCCCCTCCTCCTCCTCCGTTTCGTCCTCTCTGCCTCGCCCAATCCAACCACGTCGGCAGCGACTTCAAATTCGTGCTCCACGACGCTCTCGATTCTTCTGGAATCCTCACCTCTCACGCCAGAGATGCTAGGGAGGGATTCTGCTCGCAAATCGGCAAAATGTCTGATATAGAGAGGCAAACCAGCATTAGCATCAACCGCCGCGTTGATTTAGGGAAAACTGCTCTTTATATTGCAGCAGAGGATGACTCCCTCGTGTCGCATTCGTCCGTTCCGCTTCCAGTGGATGCTTTTATCAGTAGATTTGATGATCTTTCTATGGACTATTGTTCTCACTACAACTCTTCATTGAAATCGTCGCCCGAGAGGTTGTTGGAGAGTTTGGAAAATTATTTGTACATACACAAGGTTTTGACACATCGTTCAGGATCAACTGCAATGCTATCACTAATATACTCAGAAATTCTGAAAATGCTTCGCTTATGGGGACTTTTGGATTTTGATGTGGAGATATTCTTTCCTCACGATCCTCATAGTCTTCCTAGAGGCTATCCCAAAAAGAAAAGCAAGGAATCTGATCATGCACACATAATGACATCACAAGCCCTTTTGGTTGAGATTCTAAGAAATTTGAAGGATGCTTTCTGGCCTTTTCAGCATGATCAATCAGGGAATTTATTCTTAAGGGCTGCACGGGCTGCTAACTTCATTGATGCATCAAATGCTGATGAAGACAGTGGCTTTCAGCTTGCATCTGCTAAGGCTGCTCAACATAGGCTAGATCGTGGTGTTTGGACCAGTGTTAATTTTGGGGATATGAGGCGTGCATTATCTGCTTGCGAACGTCTTATTCTCCTCGAAGCTGATTCAAAGGAATTGAGAGATTATAGCATTCTTCTCTACCATTGTGGATTTTATGAGCAATCACTTGAATTTTTAAAGTTGTATCAGGACACAAAGACTCGCCCACAACTTAGACGATCATCTAATTCATCAACCAATCTAGAGGAAGATGCTGTACAGAAGTTAATGATACGCCTTAAACTAATTTTGATGGAGGATGGTTGGAGCAGGCCTTCATATGTAAGAAACTTTCTTGGTAATGATGAAGACAAGTTAGTGTGGCACCCTCACCGCAAAGGTTTGTTTACTACTAATAGTGCTTATTATGTAGCAAGTGATATTGCTCTCAATCAGGTTATTCACCCTCCTCAGCCTGATGGTTTTCAAAGATTGTGGAAGACAATTTGGGAGGCAAAAGTTCCCAGTAAGGTAGCTGTTAGTGTTTGGAGAGCTCGTCTAGTTACTAGAGGATATGAGGGAGAAACATCTTATGTTTTATGTGATTGTCCACTGGAATCGGTTGGTCATGTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transglut_core2

Protein Analysis

498

Amino Acids

56.45

Weight (kDa)

7.03

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1242
AccII CGCG 1 cut(s) 321
AciI CCGC 4 cut(s) 316, 319, 392, 1252
AclWI GGATC 2 cut(s) 553, 638
AcsI RAATTY 7 cut(s) 83, 92, 185, 579, 745, 790, 1049
AfaI GTAC 2 cut(s) 515, 1137
AflII CTTAAG 1 cut(s) 799
AgsI TTSAA 7 cut(s) 91, 184, 469, 751, 987, 1049, 1346
AjiI CACGTC 1 cut(s) 169
AluBI AGCT 6 cut(s) 9, 20, 865, 980, 1394, 1412
AluI AGCT 6 cut(s) 9, 20, 865, 980, 1394, 1412
Alw21I GWGCWC 2 cut(s) 195, 1414
AlwI GGATC 2 cut(s) 553, 638
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 3 cut(s) 41, 764, 1190
ApeKI GCWGC 6 cut(s) 6, 174, 353, 806, 815, 881
ApoI RAATTY 7 cut(s) 83, 92, 185, 579, 745, 790, 1049
ArsI GACNNNNNNTTYG 2 cut(s) 170, 202
Asp700I GAANNNNTTC 1 cut(s) 794
AspS9I GGNCC 2 cut(s) 51, 912
AsuHPI GGTGA 3 cut(s) 217, 1241, 1313
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 2 cut(s) 51, 912
BanI GGYRCC 1 cut(s) 1242
BanII GRGCYC 1 cut(s) 1414
BarI GAAGNNNNNNTAC 2 cut(s) 1119, 1151
BauI CACGAG 1 cut(s) 371
BbsI GAAGAC 4 cut(s) 647, 855, 1233, 1364
Bbv12I GWGCWC 2 cut(s) 195, 1414
BbvCI CCTCAGC 1 cut(s) 1329
BbvI GCAGC 6 cut(s) 18, 186, 365, 793, 802, 868
BccI CCATC 3 cut(s) 1165, 1172, 1331
BclI TGATCA 2 cut(s) 694, 778
BfaI CTAG 6 cut(s) 246, 660, 896, 1121, 1418, 1425
BfrI CTTAAG 1 cut(s) 799
BisI GCNGC 7 cut(s) 7, 175, 319, 354, 807, 816, 882
BlsI GCNGC 7 cut(s) 8, 176, 320, 355, 808, 817, 883
Bme18I GGWCC 2 cut(s) 51, 912
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 51, 912
BmiI GGNNCC 1 cut(s) 1244
BmrI ACTGGG 1 cut(s) 1377
BmsI GCATC 8 cut(s) 232, 318, 394, 745, 820, 842, 878, 1120
BmuI ACTGGG 1 cut(s) 1377
BpiI GAAGAC 4 cut(s) 647, 855, 1233, 1364
BplI GAGNNNNNCTC 2 cut(s) 350, 382
Bpu10I CCTNAGC 2 cut(s) 876, 1329
BsaJI CCNNGG 1 cut(s) 38
BsaXI ACNNNNNCTCC 2 cut(s) 1399, 1429
Bse1I ACTGG 4 cut(s) 398, 915, 1383, 1476
Bse3DI GCAATG 1 cut(s) 561
BseDI CCNNGG 1 cut(s) 38
BseGI GGATG 4 cut(s) 367, 409, 760, 1183
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 3 cut(s) 61, 588, 1343
BseNI ACTGG 4 cut(s) 398, 915, 1383, 1476
BseRI GAGGAG 7 cut(s) 58, 99, 114, 117, 120, 962, 1317
BseXI GCAGC 6 cut(s) 18, 186, 365, 793, 802, 868
BseYI CCCAGC 1 cut(s) 9
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 3 cut(s) 43, 766, 1192
BshNI GGYRCC 1 cut(s) 1242
BsiHKAI GWGCWC 2 cut(s) 195, 1414
BsiHKCI CYCGRG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 618
BsmI GAATGC 2 cut(s) 379, 1008
BsnI GGCC 3 cut(s) 43, 766, 1192
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 2 cut(s) 195, 1414
Bsp1407I TGTACA 2 cut(s) 513, 1135
Bsp143I GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 4 cut(s) 316, 319, 392, 1252
BspANI GGCC 3 cut(s) 43, 766, 1192
BspCNI CTCAG 3 cut(s) 60, 587, 1342
BspFNI CGCG 1 cut(s) 321
BspLI GGNNCC 1 cut(s) 1244
BspPI GGATC 2 cut(s) 553, 638
BspT107I GGYRCC 1 cut(s) 1242
BspTI CTTAAG 1 cut(s) 799
BsrDI GCAATG 1 cut(s) 561
BsrGI TGTACA 2 cut(s) 513, 1135
BsrI ACTGG 4 cut(s) 398, 915, 1383, 1476
BssECI CCNNGG 1 cut(s) 38
BssMI GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
BssSI CACGAG 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 854
Bst6I CTCTTC 2 cut(s) 67, 466
BstAFI CTTAAG 1 cut(s) 799
BstAPI GCANNNNNTGC 3 cut(s) 815, 839, 950
BstAUI TGTACA 2 cut(s) 513, 1135
BstC8I GCNNGC 5 cut(s) 263, 867, 942, 955, 1190
BstDEI CTNAG 6 cut(s) 47, 574, 740, 876, 1091, 1329
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 367, 409, 760, 1183
BstFNI CGCG 1 cut(s) 321
BstKTI GATC 7 cut(s) 430, 548, 646, 697, 781, 901, 1100
BstMBI GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
BstMWI GCNNNNNNNGC 8 cut(s) 242, 315, 772, 812, 815, 839, 881, 950
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 6 cut(s) 18, 186, 365, 793, 802, 868
BstV2I GAAGAC 4 cut(s) 647, 855, 1233, 1364
BsuRI GGCC 3 cut(s) 43, 766, 1192
BtgI CCRYGG 1 cut(s) 38
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 4 cut(s) 367, 409, 760, 1183
BtsIMutI CAGTG 4 cut(s) 405, 859, 922, 1469
Cac8I GCNNGC 5 cut(s) 263, 867, 942, 955, 1190
Cfr13I GGNCC 2 cut(s) 51, 912
CseI GACGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 514, 1136
CspCI CAANNNNNGTGG 2 cut(s) 1334, 1369
CviAII CATG 5 cut(s) 39, 102, 698, 776, 1487
CviQI GTAC 2 cut(s) 514, 1136
DdeI CTNAG 6 cut(s) 47, 574, 740, 876, 1091, 1329
DpnI GATC 7 cut(s) 429, 547, 645, 696, 780, 900, 1099
DpnII GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
DraI TTTAAA 1 cut(s) 1056
Eam1104I CTCTTC 2 cut(s) 67, 466
EarI CTCTTC 2 cut(s) 67, 466
Ecl136II GAGCTC 1 cut(s) 1412
Eco130I CCWWGG 1 cut(s) 38
Eco147I AGGCCT 1 cut(s) 1192
Eco24I GRGCYC 1 cut(s) 1414
Eco47I GGWCC 2 cut(s) 51, 912
Eco53kI GAGCTC 1 cut(s) 1412
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 1412
EcoO109I RGGNCCY 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 38
EcoT22I ATGCAT 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 1414
ErhI CCWWGG 1 cut(s) 38
FaeI CATG 5 cut(s) 42, 105, 701, 779, 1490
FaqI GGGAC 1 cut(s) 618
FatI CATG 5 cut(s) 38, 101, 697, 775, 1486
FauNDI CATATG 1 cut(s) 1198
FbaI TGATCA 2 cut(s) 694, 778
Fnu4HI GCNGC 7 cut(s) 7, 175, 319, 354, 807, 816, 882
FokI GGATG 4 cut(s) 374, 416, 767, 1190
FriOI GRGCYC 1 cut(s) 1414
Fsp4HI GCNGC 7 cut(s) 7, 175, 319, 354, 807, 816, 882
FspBI CTAG 6 cut(s) 246, 660, 896, 1121, 1418, 1425
GluI GCNGC 7 cut(s) 7, 175, 319, 354, 807, 816, 882
GsaI CCCAGC 1 cut(s) 13
HaeIII GGCC 3 cut(s) 43, 766, 1192
HgaI GACGC 1 cut(s) 209
Hin1II CATG 5 cut(s) 42, 105, 701, 779, 1490
HinfI GANTC 9 cut(s) 209, 219, 255, 365, 689, 736, 983, 1078, 1475
HphI GGTGA 3 cut(s) 217, 1241, 1313
Hpy166II GTNNAC 2 cut(s) 1266, 1469
Hpy188I TCNGA 4 cut(s) 283, 577, 585, 694
Hpy188III TCNNGA 7 cut(s) 49, 206, 216, 543, 641, 1067, 1121
Hpy8I GTNNAC 2 cut(s) 1266, 1469
Hpy99I CGWCG 3 cut(s) 173, 203, 478
HpyAV CCTTC 4 cut(s) 87, 123, 745, 1203
HpyCH4III ACNGT 1 cut(s) 854
HpyCH4IV ACGT 2 cut(s) 168, 961
HpyCH4V TGCA 7 cut(s) 353, 554, 701, 809, 833, 869, 944
HpyF10VI GCNNNNNNNGC 8 cut(s) 242, 315, 772, 812, 815, 839, 881, 950
HpyF3I CTNAG 6 cut(s) 47, 574, 740, 876, 1091, 1329
HpySE526I ACGT 2 cut(s) 168, 961
Hsp92II CATG 5 cut(s) 42, 105, 701, 779, 1490
Ksp22I TGATCA 2 cut(s) 694, 778
Kzo9I GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
LmnI GCTCC 3 cut(s) 17, 198, 1185
Lsp1109I GCAGC 6 cut(s) 18, 186, 365, 793, 802, 868
LweI GCATC 8 cut(s) 232, 318, 394, 745, 820, 842, 878, 1120
MaeI CTAG 6 cut(s) 246, 660, 896, 1121, 1418, 1425
MaeII ACGT 2 cut(s) 168, 961
MaeIII GTNAC 1 cut(s) 1420
MalI GATC 7 cut(s) 429, 547, 645, 696, 780, 900, 1099
MboI GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
MhlI GDGCHC 2 cut(s) 195, 1414
MlyI GAGTC 2 cut(s) 359, 1072
MmeI TCCRAC 3 cut(s) 186, 471, 1163
Mph1103I ATGCAT 1 cut(s) 835
MroXI GAANNNNTTC 1 cut(s) 794
MseI TTAA 5 cut(s) 800, 921, 1055, 1145, 1158
MspA1I CMGCKG 1 cut(s) 9
MspCI CTTAAG 1 cut(s) 799
Mva1269I GAATGC 2 cut(s) 379, 1008
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 8 cut(s) 242, 315, 772, 812, 815, 839, 881, 950
NcoI CCATGG 1 cut(s) 38
NdeI CATATG 1 cut(s) 1198
NdeII GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
NlaIII CATG 5 cut(s) 42, 105, 701, 779, 1490
NlaIV GGNNCC 1 cut(s) 1244
NsiI ATGCAT 1 cut(s) 835
PceI AGGCCT 1 cut(s) 1192
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 195, 204
PctI GAATGC 2 cut(s) 379, 1008
PdmI GAANNNNTTC 1 cut(s) 794
PfeI GAWTC 7 cut(s) 209, 219, 255, 689, 736, 983, 1475
PkrI GCNGC 7 cut(s) 8, 176, 320, 355, 808, 817, 883
PleI GAGTC 2 cut(s) 359, 1072
PpsI GAGTC 2 cut(s) 359, 1072
PpuMI RGGWCCY 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 1414
Psp5II RGGWCCY 1 cut(s) 51
PspFI CCCAGC 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 1244
PspPI GGNCC 2 cut(s) 51, 912
PspPPI RGGWCCY 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 9
RsaI GTAC 2 cut(s) 515, 1137
RsaNI GTAC 2 cut(s) 514, 1136
SacI GAGCTC 1 cut(s) 1414
SaqAI TTAA 5 cut(s) 800, 921, 1055, 1145, 1158
SatI GCNGC 7 cut(s) 7, 175, 319, 354, 807, 816, 882
Sau3AI GATC 7 cut(s) 427, 545, 643, 694, 778, 898, 1097
Sau96I GGNCC 2 cut(s) 51, 912
SchI GAGTC 2 cut(s) 359, 1072
SduI GDGCHC 2 cut(s) 195, 1414
SfaNI GCATC 8 cut(s) 232, 318, 394, 745, 820, 842, 878, 1120
SinI GGWCC 2 cut(s) 51, 912
SmlI CTYRAG 1 cut(s) 799
SmoI CTYRAG 1 cut(s) 799
SseBI AGGCCT 1 cut(s) 1192
SsiI CCGC 4 cut(s) 316, 319, 392, 1252
SspMI CTAG 6 cut(s) 246, 660, 896, 1121, 1418, 1425
SstI GAGCTC 1 cut(s) 1414
StuI AGGCCT 1 cut(s) 1192
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 1 cut(s) 854
TaiI ACGT 2 cut(s) 171, 964
TaqI TCGA 2 cut(s) 207, 975
TatI WGTACW 2 cut(s) 513, 1135
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 7 cut(s) 209, 219, 255, 689, 736, 983, 1475
Tru1I TTAA 5 cut(s) 800, 921, 1055, 1145, 1158
Tru9I TTAA 5 cut(s) 800, 921, 1055, 1145, 1158
TscAI CASTG 4 cut(s) 405, 859, 922, 1476
TseI GCWGC 6 cut(s) 6, 174, 353, 806, 815, 881
TspDTI ATGAA 7 cut(s) 90, 453, 814, 861, 1098, 1185, 1239
TspGWI ACGGA 2 cut(s) 123, 376
TspRI CASTG 4 cut(s) 405, 859, 922, 1476
Vha464I CTTAAG 1 cut(s) 799
VpaK11BI GGWCC 2 cut(s) 51, 912
XapI RAATTY 7 cut(s) 83, 92, 185, 579, 745, 790, 1049
XbaI TCTAGA 1 cut(s) 1120
XmnI GAANNNNTTC 1 cut(s) 794
XspI CTAG 6 cut(s) 246, 660, 896, 1121, 1418, 1425
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.