Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002560

Physical Location & Seq

Genomic Coordinates Reverse (-)

34709309 .. 34714920

Gene Structure 5612 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1291400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGCGGCAGTTTTGCTGGGCAAGCTTCTGATTGCCGTCGTGCTACTGATGCCGCATGCGGTGGTTCTGTGCAGTTTTCCCGCCACTCTAACTCTGGAGAGAGCTTTTCCAACAAATCACAAAGTGGAAATGAGTCAACTCAAAGCTCGAGATCGAGTCAGGCATGGAAGGATGTTGCAGCAGTCGCCAAGTGGTGTCGTCAGTTTCCCTGTTGGAGGAACCTTCAATCCATACCTTGTCGGGCTTTATTTTACAAGCGTGAAATTGGGTACACCTGCAACAGAGTACTATGTGCAGATTGATACCGGAAGTGATGTTTTGTGGGTTGGCTGCACTTCCTGCAATGGCTGCCCCCAATCGACTGGACTCAAAATCCAGCTGAAAGATTTTGATCCTAGTAGCTCCTCAACAGCCTCGTTGGTCTCTTGTTCGGATAAACTGTGTACCCTTGGACAGCAGACTCAAGATTCTCTCTGTGATGCACAGAATAAGCAGTGTACTTACGATTTCGAATACGGAGATGGCAGTGGCACATCAGGCTACTATGTATCAGACTTGTTGCTTCTTGACACAGCCGGCGGTGATCAGTCTGGTACCTCAAACTCTTCAGCTAAAGTTGTCTTTGGTTGTAGCACCTCAGCAACAGGGGTCTTGCAGAAAACAGCTGCAGCAGTTGATGGGATTTTTGGATTTGGGCAGCAGGAACTTTCCGTCATCTCCCAGCTGTCTTCACAGGGATTAGCACCAAAAGTATTTTCTCACTGCTTTAGAGGAGATGACACCGGTGGAGGGATTTTGGTTCTTGGCGAGATCACGGAACCAAACATAGTTTATAGCCCACTTGTCCAGAATCAGCCTCACTATAACTTAAATCTGAAGAACATTGCTGTTAATGGCCAAATACTGGACATTGCTTCATCAGTGTTTGCAACATCGGACACCCAAGGAACCATAGTTGACTCCGGGACAACTTTGGCATACCTTGCAGAAAATGCCTATGATCCTTTCGTCAACGCTATAACAAGCGCTGTTTCACAATCCGTGACCCCTGTCACCTCTCAGGGAGAGCAGTGCTACATAGTCACTTCCAGCATCTCGACTGCATTTCCTCAAGTTAGCTTGAACTTTGATGGCAATGCATCCATGATTCTAAGACCCGAGGACTACCTTTTACAGCAGAACTCTATGAATGGTGATGAAGTATGGTGCATCGGCATCGCAAAAAATCAGGGTCAACAAACTGTATTAGGAGACCTCATCTTAAAAGACAAGATCATTGTGTATGATTTGGCGAATCAACGGATTGGTTGGGCTCAATATAACTGTTCACAGTCAGTCAGCGTTTCTACGAATACAAGTACCGGAACTGCATCTGTTAGTCCAGGGTCGATAGACCTTAGTGGTTCAGCGCGCAACGACCCCTTCAAGCTGATAACAACAAGCGTGATGGTTTTCCTTGTTCTCACGTCAATCATTCACATTGTCCCATCGCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

499

Amino Acids

52.78

Weight (kDa)

4.75

Isoelectric Point (pI)

40.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TAXi_N	PF14543	83 - 269	2.2e-42	Xylanase inhibitor N-terminal
Asp	PF00026	83 - 439	9.8e-29	Eukaryotic aspartyl protease
TAXi_C	PF14541	287 - 438	1.2e-26	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002560)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22850
fragaria_vesca	FvH4_3g30200 FvH4_3g30200 FvH4_3g30200 FvH4_7g02560 FvH4_7g02560 FvH4_7g02560 FvH4_7g02560
malus_domestica	MD02G1291400.v1.1 MD03G1130700.v1.1 MD11G1152500.v1.1
prunus_persica	Prupe.2G031400_v2.0.a1 Prupe.6G115500_v2.0.a1 Prupe.6G115500_v2.0.a1
pyrus_communis	pycom02g24630 pycom02g24640 pycom11g12500
rosa_chinensis	RchiOBHm_Chr1g0321281 RchiOBHm_Chr5g0055981
rosa_laevigata	RLG00000030431 RLG00000035040
rosa_multiflora	Rmu_co8118894.1_g000001 Rmu_sc0029150.1_g000001
rosa_roxburghii	Rroxscaffold_160G00433920 Rroxscaffold_1G00024290 Rroxscaffold_4G00327850
rosa_rugosa	Rorug01G0031900 Rorug05G0296800 Rorug05G0296900
rosa_samantha	Rh1AG044000 Rh1BG041700 Rh1CG046600 Rh1DG051200 Rh5AG367900 Rh5BG378300 Rh5CG401700 Rh5DG391400
rosa_wichuraiana	Rw0G006980 Rw1G003880 Rw5G034530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	283
Acc36I	ACCTGC	1 cut(s)	283
Acc65I	GGTACC	1 cut(s)	593
AccB1I	GGYRCC	1 cut(s)	593
AccB7I	CCANNNNNTGG	1 cut(s)	904
AccII	CGCG	1 cut(s)	1411
AciI	CCGC	5 cut(s)	5, 53, 59, 81, 579
AclWI	GGATC	2 cut(s)	386, 995
AcoI	YGGCCR	1 cut(s)	895
AcuI	CTGAAG	2 cut(s)	591, 896
AdeI	CACNNNGTG	1 cut(s)	124
AfaI	GTAC	6 cut(s)	271, 287, 445, 499, 595, 1360
AfeI	AGCGCT	1 cut(s)	1027
AfiI	CCNNNNNNNGG	3 cut(s)	215, 789, 904
AgeI	ACCGGT	1 cut(s)	782
AgsI	TTSAA	3 cut(s)	226, 1123, 1426
AjiI	CACGTC	1 cut(s)	1467
AjnI	CCWGG	1 cut(s)	1381
AjuI	GAANNNNNNNTTGG	2 cut(s)	739, 771
AloI	GAACNNNNNNTCC	2 cut(s)	211, 243
Alw26I	GTCTC	2 cut(s)	427, 1245
AlwI	GGATC	2 cut(s)	386, 995
Ama87I	CYCGRG	2 cut(s)	147, 1157
Aor51HI	AGCGCT	1 cut(s)	1027
AoxI	GGCC	1 cut(s)	895
ApeKI	GCWGC	6 cut(s)	178, 330, 348, 665, 668, 697
AsiGI	ACCGGT	1 cut(s)	782
Asp718I	GGTACC	1 cut(s)	593
AspLEI	GCGC	3 cut(s)	1028, 1411, 1413
AsuC2I	CCSGG	1 cut(s)	964
AsuHPI	GGTGA	3 cut(s)	593, 1045, 1205
AsuII	TTCGAA	1 cut(s)	510
AvaI	CYCGRG	2 cut(s)	147, 1157
BalI	TGGCCA	1 cut(s)	897
BanI	GGYRCC	1 cut(s)	593
BanII	GRGCYC	1 cut(s)	1315
BbsI	GAAGAC	1 cut(s)	720
BbvCI	CCTCAGC	1 cut(s)	637
BbvI	GCAGC	6 cut(s)	190, 317, 335, 652, 680, 709
BccI	CCATC	5 cut(s)	515, 671, 1124, 1441, 1495
BceAI	ACGGC	1 cut(s)	20
BcgI	CGANNNNNNTGC	2 cut(s)	1198, 1232
BciT130I	CCWGG	1 cut(s)	1383
BclI	TGATCA	1 cut(s)	583
BcnI	CCSGG	1 cut(s)	964
BcoDI	GTCTC	2 cut(s)	427, 1245
BfaI	CTAG	1 cut(s)	396
BfmI	CTRYAG	2 cut(s)	666, 1493
BfoI	RGCGCY	1 cut(s)	1029
BfuAI	ACCTGC	1 cut(s)	283
BisI	GCNGC	8 cut(s)	6, 53, 179, 331, 349, 666, 669, 698
BlsI	GCNGC	8 cut(s)	7, 54, 180, 332, 350, 667, 670, 699
BmcAI	AGTACT	1 cut(s)	287
Bme1390I	CCNGG	2 cut(s)	964, 1383
BmeT110I	CYCGRG	2 cut(s)	147, 1157
BmgBI	CACGTC	1 cut(s)	1467
BmiI	GGNNCC	4 cut(s)	220, 595, 819, 949
BmrFI	CCNGG	2 cut(s)	964, 1383
BmsI	GCATC	7 cut(s)	39, 469, 1101, 1148, 1218, 1224, 1379
BoxI	GACNNNNGTC	1 cut(s)	1049
BpiI	GAAGAC	1 cut(s)	720
BpmI	CTGGAG	1 cut(s)	116
Bpu10I	CCTNAGC	1 cut(s)	637
Bpu14I	TTCGAA	1 cut(s)	510
BpuEI	CTTGAG	2 cut(s)	447, 1095
BpuMI	CCSGG	1 cut(s)	964
BsaBI	GATNNNNATC	1 cut(s)	390
BsaI	GGTCTC	2 cut(s)	427, 1245
BsaJI	CCNNGG	4 cut(s)	448, 943, 1158, 1382
BsaWI	WCCGGW	3 cut(s)	305, 782, 1361
Bsc4I	CCNNNNNNNGG	3 cut(s)	215, 789, 904
Bse118I	RCCGGY	2 cut(s)	575, 782
Bse1I	ACTGG	2 cut(s)	367, 909
Bse3DI	GCAATG	4 cut(s)	349, 882, 909, 1141
Bse8I	GATNNNNATC	1 cut(s)	390
BseBI	CCWGG	1 cut(s)	1383
BseDI	CCNNGG	4 cut(s)	448, 943, 1158, 1382
BseGI	GGATG	2 cut(s)	177, 1139
BseJI	GATNNNNATC	1 cut(s)	390
BseLI	CCNNNNNNNGG	3 cut(s)	215, 789, 904
BseMI	GCAATG	4 cut(s)	349, 882, 909, 1141
BseMII	CTCAG	2 cut(s)	651, 1073
BseNI	ACTGG	2 cut(s)	367, 909
BsePI	GCGCGC	1 cut(s)	1409
BseRI	GAGGAG	2 cut(s)	394, 786
BseXI	GCAGC	6 cut(s)	190, 317, 335, 652, 680, 709
BseYI	CCCAGC	2 cut(s)	16, 720
BsgI	GTGCAG	3 cut(s)	91, 314, 316
Bsh1236I	CGCG	1 cut(s)	1411
BshFI	GGCC	1 cut(s)	897
BshNI	GGYRCC	1 cut(s)	593
BshTI	ACCGGT	1 cut(s)	782
BsiHKCI	CYCGRG	2 cut(s)	147, 1157
BsiSI	CCGG	5 cut(s)	306, 576, 783, 963, 1362
BslFI	GGGAC	2 cut(s)	979, 1469
BslI	CCNNNNNNNGG	3 cut(s)	215, 789, 904
BsmAI	GTCTC	2 cut(s)	427, 1245
BsmFI	GGGAC	2 cut(s)	979, 1469
BsnI	GGCC	1 cut(s)	897
Bso31I	GGTCTC	2 cut(s)	427, 1245
BsoBI	CYCGRG	2 cut(s)	147, 1157
Bsp119I	TTCGAA	1 cut(s)	510
Bsp1286I	GDGCHC	1 cut(s)	1315
Bsp143I	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
BspACI	CCGC	5 cut(s)	5, 53, 59, 81, 579
BspANI	GGCC	1 cut(s)	897
BspCNI	CTCAG	2 cut(s)	650, 1072
BspFNI	CGCG	1 cut(s)	1411
BspLI	GGNNCC	4 cut(s)	220, 595, 819, 949
BspMAI	CTGCAG	1 cut(s)	670
BspMI	ACCTGC	1 cut(s)	283
BspPI	GGATC	2 cut(s)	386, 995
BspT104I	TTCGAA	1 cut(s)	510
BspT107I	GGYRCC	1 cut(s)	593
BspTNI	GGTCTC	2 cut(s)	427, 1245
BsrDI	GCAATG	4 cut(s)	349, 882, 909, 1141
BsrFI	RCCGGY	2 cut(s)	575, 782
BsrI	ACTGG	2 cut(s)	367, 909
BssAI	RCCGGY	2 cut(s)	575, 782
BssECI	CCNNGG	4 cut(s)	448, 943, 1158, 1382
BssHII	GCGCGC	1 cut(s)	1409
BssMI	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
BssT1I	CCWWGG	2 cut(s)	448, 943
Bst2UI	CCWGG	1 cut(s)	1383
Bst4CI	ACNGT	4 cut(s)	441, 1243, 1325, 1332
Bst6I	CTCTTC	1 cut(s)	610
BstAPI	GCANNNNNTGC	4 cut(s)	339, 348, 983, 992
BstBI	TTCGAA	1 cut(s)	510
BstC8I	GCNNGC	4 cut(s)	23, 57, 577, 1411
BstDEI	CTNAG	4 cut(s)	637, 1059, 1151, 1397
BstENI	CCTNNNNNAGG	1 cut(s)	213
BstF5I	GGATG	2 cut(s)	177, 1139
BstFNI	CGCG	1 cut(s)	1411
BstH2I	RGCGCY	1 cut(s)	1029
BstHHI	GCGC	3 cut(s)	1028, 1411, 1413
BstKTI	GATC	6 cut(s)	154, 394, 586, 813, 1003, 1275
BstMAI	GTCTC	2 cut(s)	427, 1245
BstMBI	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
BstMWI	GCNNNNNNNGC	8 cut(s)	22, 49, 184, 339, 348, 537, 983, 992
BstNI	CCWGG	1 cut(s)	1383
BstNSI	RCATGY	1 cut(s)	59
BstPAI	GACNNNNGTC	1 cut(s)	1049
BstSCI	CCNGG	2 cut(s)	962, 1381
BstSFI	CTRYAG	2 cut(s)	666, 1493
BstUI	CGCG	1 cut(s)	1411
BstV1I	GCAGC	6 cut(s)	190, 317, 335, 652, 680, 709
BstV2I	GAAGAC	1 cut(s)	720
BstXI	CCANNNNNNTGG	1 cut(s)	362
BsuRI	GGCC	1 cut(s)	897
BtgZI	GCGATG	2 cut(s)	1201, 1473
BtrI	CACGTC	1 cut(s)	1467
BtsCI	GGATG	2 cut(s)	177, 1139
BtsI	GCAGTG	4 cut(s)	500, 532, 760, 1076
BtsIMutI	CAGTG	5 cut(s)	500, 532, 760, 927, 1076
BveI	ACCTGC	1 cut(s)	283
Cac8I	GCNNGC	4 cut(s)	23, 57, 577, 1411
CfoI	GCGC	3 cut(s)	1028, 1411, 1413
Cfr10I	RCCGGY	2 cut(s)	575, 782
Csp6I	GTAC	6 cut(s)	270, 286, 444, 498, 594, 1359
CspAI	ACCGGT	1 cut(s)	782
CviAII	CATG	3 cut(s)	56, 164, 1144
CviQI	GTAC	6 cut(s)	270, 286, 444, 498, 594, 1359
DdeI	CTNAG	4 cut(s)	637, 1059, 1151, 1397
DpnI	GATC	6 cut(s)	153, 393, 585, 812, 1002, 1274
DpnII	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
DraIII	CACNNNGTG	1 cut(s)	124
EaeI	YGGCCR	1 cut(s)	895
Eam1104I	CTCTTC	1 cut(s)	610
EarI	CTCTTC	1 cut(s)	610
Eco130I	CCWWGG	2 cut(s)	448, 943
Eco24I	GRGCYC	1 cut(s)	1315
Eco31I	GGTCTC	2 cut(s)	427, 1245
Eco47III	AGCGCT	1 cut(s)	1027
Eco57I	CTGAAG	2 cut(s)	591, 896
Eco88I	CYCGRG	2 cut(s)	147, 1157
EcoNI	CCTNNNNNAGG	1 cut(s)	213
EcoRII	CCWGG	1 cut(s)	1381
EcoT14I	CCWWGG	2 cut(s)	448, 943
EcoT22I	ATGCAT	1 cut(s)	1141
EcoT38I	GRGCYC	1 cut(s)	1315
ErhI	CCWWGG	2 cut(s)	448, 943
FaeI	CATG	3 cut(s)	59, 167, 1147
FaqI	GGGAC	2 cut(s)	979, 1469
FatI	CATG	3 cut(s)	55, 163, 1143
FauI	CCCGC	1 cut(s)	88
FbaI	TGATCA	1 cut(s)	583
Fnu4HI	GCNGC	8 cut(s)	6, 53, 179, 331, 349, 666, 669, 698
FokI	GGATG	2 cut(s)	184, 1126
FriOI	GRGCYC	1 cut(s)	1315
Fsp4HI	GCNGC	8 cut(s)	6, 53, 179, 331, 349, 666, 669, 698
FspBI	CTAG	1 cut(s)	396
GlaI	GCGC	3 cut(s)	1027, 1410, 1412
GluI	GCNGC	8 cut(s)	6, 53, 179, 331, 349, 666, 669, 698
GsaI	CCCAGC	2 cut(s)	20, 724
GsuI	CTGGAG	1 cut(s)	116
HaeII	RGCGCY	1 cut(s)	1029
HaeIII	GGCC	1 cut(s)	897
HapII	CCGG	5 cut(s)	306, 576, 783, 963, 1362
HhaI	GCGC	3 cut(s)	1028, 1411, 1413
Hin1II	CATG	3 cut(s)	59, 167, 1147
Hin6I	GCGC	3 cut(s)	1026, 1409, 1411
HinP1I	GCGC	3 cut(s)	1026, 1409, 1411
HincII	GTYRAC	4 cut(s)	137, 958, 1012, 1235
HindII	GTYRAC	4 cut(s)	137, 958, 1012, 1235
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	9 cut(s)	133, 156, 366, 460, 467, 850, 959, 1147, 1294
HpaII	CCGG	5 cut(s)	306, 576, 783, 963, 1362
HphI	GGTGA	3 cut(s)	593, 1045, 1205
Hpy166II	GTNNAC	8 cut(s)	137, 272, 444, 498, 958, 1012, 1235, 1328
Hpy188I	TCNGA	5 cut(s)	30, 433, 553, 876, 937
Hpy188III	TCNNGA	6 cut(s)	95, 149, 464, 566, 847, 1096
Hpy8I	GTNNAC	8 cut(s)	137, 272, 444, 498, 958, 1012, 1235, 1328
Hpy99I	CGWCG	1 cut(s)	41
HpyAV	CCTTC	3 cut(s)	162, 232, 1432
HpyCH4III	ACNGT	4 cut(s)	441, 1243, 1325, 1332
HpyCH4IV	ACGT	1 cut(s)	1466
HpyF10VI	GCNNNNNNNGC	8 cut(s)	22, 49, 184, 339, 348, 537, 983, 992
HpyF3I	CTNAG	4 cut(s)	637, 1059, 1151, 1397
HpySE526I	ACGT	1 cut(s)	1466
Hsp92II	CATG	3 cut(s)	59, 167, 1147
HspAI	GCGC	3 cut(s)	1026, 1409, 1411
KpnI	GGTACC	1 cut(s)	597
KroI	GCCGGC	1 cut(s)	575
KroNI	GCCGGC	1 cut(s)	577
Ksp22I	TGATCA	1 cut(s)	583
Kzo9I	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
LmnI	GCTCC	1 cut(s)	407
Lsp1109I	GCAGC	6 cut(s)	190, 317, 335, 652, 680, 709
LweI	GCATC	7 cut(s)	39, 469, 1101, 1148, 1218, 1224, 1379
MaeI	CTAG	1 cut(s)	396
MaeII	ACGT	1 cut(s)	1466
MaeIII	GTNAC	3 cut(s)	1042, 1051, 1081
MalI	GATC	6 cut(s)	153, 393, 585, 812, 1002, 1274
MboI	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
MboII	GAAGA	3 cut(s)	597, 720, 889
MhlI	GDGCHC	1 cut(s)	1315
MlsI	TGGCCA	1 cut(s)	897
MluCI	AATT	1 cut(s)	263
MluNI	TGGCCA	1 cut(s)	897
MlyI	GAGTC	5 cut(s)	142, 165, 360, 454, 953
MmeI	TCCRAC	2 cut(s)	134, 193
Mox20I	TGGCCA	1 cut(s)	897
Mph1103I	ATGCAT	1 cut(s)	1141
MroNI	GCCGGC	1 cut(s)	575
MscI	TGGCCA	1 cut(s)	897
MseI	TTAA	3 cut(s)	869, 891, 1262
Msp20I	TGGCCA	1 cut(s)	897
MspA1I	CMGCKG	3 cut(s)	379, 665, 724
MspI	CCGG	5 cut(s)	306, 576, 783, 963, 1362
MspR9I	CCNGG	2 cut(s)	964, 1383
MvaI	CCWGG	1 cut(s)	1383
MvnI	CGCG	1 cut(s)	1411
MwoI	GCNNNNNNNGC	8 cut(s)	22, 49, 184, 339, 348, 537, 983, 992
NaeI	GCCGGC	1 cut(s)	577
NciI	CCSGG	1 cut(s)	964
NdeII	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
NgoMIV	GCCGGC	1 cut(s)	575
NlaIII	CATG	3 cut(s)	59, 167, 1147
NlaIV	GGNNCC	4 cut(s)	220, 595, 819, 949
NmuCI	GTSAC	3 cut(s)	1042, 1051, 1081
NsiI	ATGCAT	1 cut(s)	1141
NspI	RCATGY	1 cut(s)	59
NspV	TTCGAA	1 cut(s)	510
PaeI	GCATGC	1 cut(s)	59
PaeR7I	CTCGAG	1 cut(s)	147
PaqCI	CACCTGC	1 cut(s)	283
PauI	GCGCGC	1 cut(s)	1409
PdiI	GCCGGC	1 cut(s)	577
PfeI	GAWTC	4 cut(s)	467, 850, 1147, 1294
PflMI	CCANNNNNTGG	1 cut(s)	904
PfoI	TCCNGGA	1 cut(s)	962
PinAI	ACCGGT	1 cut(s)	782
PkrI	GCNGC	8 cut(s)	7, 54, 180, 332, 350, 667, 670, 699
PleI	GAGTC	5 cut(s)	141, 164, 360, 454, 953
PpsI	GAGTC	5 cut(s)	141, 164, 360, 454, 953
PshAI	GACNNNNGTC	1 cut(s)	1049
Psp6I	CCWGG	1 cut(s)	1381
PspFI	CCCAGC	2 cut(s)	16, 720
PspGI	CCWGG	1 cut(s)	1381
PspN4I	GGNNCC	4 cut(s)	220, 595, 819, 949
PstI	CTGCAG	1 cut(s)	670
PteI	GCGCGC	1 cut(s)	1409
PvuII	CAGCTG	3 cut(s)	379, 665, 724
RsaI	GTAC	6 cut(s)	271, 287, 445, 499, 595, 1360
RsaNI	GTAC	6 cut(s)	270, 286, 444, 498, 594, 1359
SaqAI	TTAA	3 cut(s)	869, 891, 1262
SatI	GCNGC	8 cut(s)	6, 53, 179, 331, 349, 666, 669, 698
Sau3AI	GATC	6 cut(s)	151, 391, 583, 810, 1000, 1272
ScaI	AGTACT	1 cut(s)	287
SchI	GAGTC	5 cut(s)	142, 165, 360, 454, 953
ScrFI	CCNGG	2 cut(s)	964, 1383
SduI	GDGCHC	1 cut(s)	1315
SfaNI	GCATC	7 cut(s)	39, 469, 1101, 1148, 1218, 1224, 1379
SfcI	CTRYAG	2 cut(s)	666, 1493
Sfr274I	CTCGAG	1 cut(s)	147
SfuI	TTCGAA	1 cut(s)	510
SgrAI	CRCCGGYG	1 cut(s)	782
SlaI	CTCGAG	1 cut(s)	147
SmlI	CTYRAG	3 cut(s)	147, 462, 1110
SmoI	CTYRAG	3 cut(s)	147, 462, 1110
SphI	GCATGC	1 cut(s)	59
Sse9I	AATT	1 cut(s)	263
SsiI	CCGC	5 cut(s)	5, 53, 59, 81, 579
SspMI	CTAG	1 cut(s)	396
StyD4I	CCNGG	2 cut(s)	962, 1381
StyI	CCWWGG	2 cut(s)	448, 943
TaaI	ACNGT	4 cut(s)	441, 1243, 1325, 1332
TaiI	ACGT	1 cut(s)	1469
TaqI	TCGA	6 cut(s)	148, 154, 359, 510, 1097, 1388
TasI	AATT	1 cut(s)	263
TatI	WGTACW	2 cut(s)	285, 497
TauI	GCSGC	2 cut(s)	8, 55
TfiI	GAWTC	4 cut(s)	467, 850, 1147, 1294
Tru1I	TTAA	3 cut(s)	869, 891, 1262
Tru9I	TTAA	3 cut(s)	869, 891, 1262
TscAI	CASTG	5 cut(s)	500, 532, 767, 927, 1076
TseFI	GTSAC	3 cut(s)	1042, 1051, 1081
TseI	GCWGC	6 cut(s)	178, 330, 348, 665, 668, 697
Tsp45I	GTSAC	3 cut(s)	1042, 1051, 1081
TspDTI	ATGAA	3 cut(s)	906, 1202, 1212
TspGWI	ACGGA	5 cut(s)	531, 700, 830, 1030, 1315
TspRI	CASTG	5 cut(s)	500, 532, 767, 927, 1076
Van91I	CCANNNNNTGG	1 cut(s)	904
XagI	CCTNNNNNAGG	1 cut(s)	213
XceI	RCATGY	1 cut(s)	59
XcmI	CCANNNNNNNNNTGG	1 cut(s)	91
XhoI	CTCGAG	1 cut(s)	147
XspI	CTAG	1 cut(s)	396
ZrmI	AGTACT	1 cut(s)	287
Zsp2I	ATGCAT	1 cut(s)	1141

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.