MD03G1010200.v1.1

K homology RNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003736
Physical Location & Seq
Forward (+)
779653 .. 788260
8608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1010200.v1.1.491

Sequence Viewer

Length: 696 bp
ATGTCTGGTACAACGATGACACCTGCATCATCAGCTGGTACACAAAAGGTCTCCATGTTTGCTGCCAAGGCTGGATTTGTTATACCAAAAAACAAGCTGTTGGGTTCACTTGTGCCTGTCTTTCGCGGAAGTAAAAACCTAGGAACCGGTGACGCAGTTAGTGGAGAAAGTAAGAAACAGATTCAGAGGAAAACAAAATGGGGCCCTGATCTCACCCAAGATGCTTCTGTCAAAAAAGGAAGAGCTTTAGCTTATCAGGACGTTGACCAGTTGGAATTTGAAAAGCGGGAAGCCATAGATTTAAAGTTAAATCCAAGCTACAAGGCCCCACCTGATTATAAACCTTTGTTGAAAGAGGCTACAGTCCCTATTCCTGTGAAAGAATATCCTAAATATAATCTTGTTGGCCTAATATATGGTCCTGAAAGTGATAACCAGAAACAATTAGAAAAGGAAACCGGAGCCAAGATTCAAGTCTATGGTACCAAAGCAGGGACAGGACACAAGGCTGAAATAAAACCATCTGATGGGAGTGAAATCCATGGTGAATATGAAAGCTTATATGTTCATATATCAGTTGACACATATGAGAAAGTAGATGCAGCGGTTGCTGTAATTGAACTCTTAGTCACCTCTGTATCAGTGAGTTTCCTTTTTATTTCTAAACAAAAAGCTTTAAGTCCAACAGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 SF1-HH

Protein Analysis

232

Amino Acids

25.02

Weight (kDa)

9.08

Isoelectric Point (pI)

23.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH-I_KHDC4-BBP PF22675 128 - 210 8.2e-14 KHDC4/BBP-like, KH-domain type I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 339
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
Acc65I GGTACC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 527
AccII CGCG 1 cut(s) 126
AciI CCGC 3 cut(s) 126, 286, 605
AcsI RAATTY 1 cut(s) 275
AfaI GTAC 3 cut(s) 10, 40, 484
AfiI CCNNNNNNNGG 2 cut(s) 492, 527
AgeI ACCGGT 1 cut(s) 146
AgsI TTSAA 4 cut(s) 281, 352, 473, 620
AluBI AGCT 7 cut(s) 35, 97, 245, 251, 318, 558, 674
AluI AGCT 7 cut(s) 35, 97, 245, 251, 318, 558, 674
Alw26I GTCTC 1 cut(s) 55
AoxI GGCC 3 cut(s) 202, 324, 406
ApaI GGGCCC 1 cut(s) 206
ApeKI GCWGC 2 cut(s) 62, 602
ApoI RAATTY 1 cut(s) 275
AsiGI ACCGGT 1 cut(s) 146
Asp718I GGTACC 1 cut(s) 482
AspA2I CCTAGG 1 cut(s) 139
AspS9I GGNCC 4 cut(s) 202, 203, 325, 419
AsuHPI GGTGA 4 cut(s) 161, 205, 557, 622
AvaII GGWCC 1 cut(s) 419
AvrII CCTAGG 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 206
BaeI ACNNNNGTAYC 2 cut(s) 621, 654
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 49, 614
BccI CCATC 2 cut(s) 521, 529
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 360
BfuAI ACCTGC 1 cut(s) 31
BisI GCNGC 2 cut(s) 63, 603
BlnI CCTAGG 1 cut(s) 139
BlsI GCNGC 2 cut(s) 64, 604
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 4 cut(s) 202, 203, 325, 419
BmiI GGNNCC 6 cut(s) 145, 203, 204, 327, 463, 484
BmsI GCATC 3 cut(s) 35, 211, 589
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 66, 139, 541
BsaWI WCCGGW 2 cut(s) 146, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 527
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 268
BseDI CCNNGG 3 cut(s) 66, 139, 541
BseLI CCNNNNNNNGG 2 cut(s) 492, 527
BseNI ACTGG 1 cut(s) 268
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 2 cut(s) 49, 614
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 204, 326, 408
BshNI GGYRCC 1 cut(s) 482
BshTI ACCGGT 1 cut(s) 146
BsiSI CCGG 2 cut(s) 147, 459
BslFI GGGAC 2 cut(s) 350, 508
BslI CCNNNNNNNGG 2 cut(s) 492, 527
BsmAI GTCTC 1 cut(s) 55
BsmFI GGGAC 2 cut(s) 350, 508
BsnI GGCC 3 cut(s) 204, 326, 408
Bso31I GGTCTC 1 cut(s) 55
Bsp120I GGGCCC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 541
BspACI CCGC 3 cut(s) 126, 286, 605
BspANI GGCC 3 cut(s) 204, 326, 408
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 6 cut(s) 145, 203, 204, 327, 463, 484
BspMI ACCTGC 1 cut(s) 31
BspQI GCTCTTC 1 cut(s) 235
BspT107I GGYRCC 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 146
BsrI ACTGG 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 66, 139, 541
BssMI GATC 1 cut(s) 208
BssT1I CCWWGG 3 cut(s) 66, 139, 541
Bst4CI ACNGT 2 cut(s) 364, 688
Bst6I CTCTTC 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 608
BstDEI CTNAG 1 cut(s) 625
BstDSI CCRYGG 1 cut(s) 541
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 3 cut(s) 32, 68, 608
BstSFI CTRYAG 1 cut(s) 360
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 49, 614
BsuRI GGCC 3 cut(s) 204, 326, 408
BtgI CCRYGG 1 cut(s) 541
BtsIMutI CAGTG 1 cut(s) 648
BveI ACCTGC 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 4 cut(s) 202, 203, 325, 419
CseI GACGC 1 cut(s) 161
Csp6I GTAC 3 cut(s) 9, 39, 483
CspAI ACCGGT 1 cut(s) 146
CviAII CATG 2 cut(s) 55, 542
CviQI GTAC 3 cut(s) 9, 39, 483
DdeI CTNAG 1 cut(s) 625
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
DraI TTTAAA 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 235
EarI CTCTTC 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 66, 139, 541
Eco24I GRGCYC 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 419
EcoO109I RGGNCCY 3 cut(s) 202, 203, 325
EcoT14I CCWWGG 3 cut(s) 66, 139, 541
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 3 cut(s) 66, 139, 541
FaeI CATG 2 cut(s) 58, 545
FaqI GGGAC 2 cut(s) 350, 508
FatI CATG 2 cut(s) 54, 541
FauI CCCGC 1 cut(s) 279
FauNDI CATATG 1 cut(s) 586
Fnu4HI GCNGC 2 cut(s) 63, 603
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 2 cut(s) 63, 603
FspBI CTAG 1 cut(s) 140
GluI GCNGC 2 cut(s) 63, 603
HaeIII GGCC 3 cut(s) 204, 326, 408
HapII CCGG 2 cut(s) 147, 459
HgaI GACGC 1 cut(s) 161
Hin1II CATG 2 cut(s) 58, 545
HincII GTYRAC 2 cut(s) 265, 580
HindII GTYRAC 2 cut(s) 265, 580
HindIII AAGCTT 2 cut(s) 556, 672
HinfI GANTC 2 cut(s) 181, 469
HpaII CCGG 2 cut(s) 147, 459
HphI GGTGA 4 cut(s) 161, 205, 557, 622
Hpy166II GTNNAC 4 cut(s) 41, 107, 265, 580
Hpy188I TCNGA 2 cut(s) 186, 526
Hpy188III TCNNGA 2 cut(s) 257, 422
Hpy8I GTNNAC 4 cut(s) 41, 107, 265, 580
HpyCH4III ACNGT 2 cut(s) 364, 688
HpyCH4IV ACGT 1 cut(s) 261
HpyCH4V TGCA 2 cut(s) 26, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 68, 608
HpyF3I CTNAG 1 cut(s) 625
HpySE526I ACGT 1 cut(s) 261
Hsp92II CATG 2 cut(s) 58, 545
KpnI GGTACC 1 cut(s) 486
Kzo9I GATC 1 cut(s) 208
LguI GCTCTTC 1 cut(s) 235
LmnI GCTCC 1 cut(s) 461
Lsp1109I GCAGC 2 cut(s) 49, 614
LweI GCATC 3 cut(s) 35, 211, 589
MaeI CTAG 1 cut(s) 140
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 149, 628
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 3 cut(s) 275, 443, 615
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 3 cut(s) 180, 349, 643
MseI TTAA 4 cut(s) 302, 308, 677, 694
MspA1I CMGCKG 2 cut(s) 35, 605
MspI CCGG 2 cut(s) 147, 459
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 3 cut(s) 32, 68, 608
NcoI CCATGG 1 cut(s) 541
NdeI CATATG 1 cut(s) 586
NdeII GATC 1 cut(s) 208
NlaIII CATG 2 cut(s) 58, 545
NlaIV GGNNCC 6 cut(s) 145, 203, 204, 327, 463, 484
NmuCI GTSAC 2 cut(s) 149, 628
PaqCI CACCTGC 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 235
PfeI GAWTC 2 cut(s) 181, 469
PflMI CCANNNNNTGG 1 cut(s) 527
PinAI ACCGGT 1 cut(s) 146
PkrI GCNGC 2 cut(s) 64, 604
PsiI TTATAA 1 cut(s) 339
PspN4I GGNNCC 6 cut(s) 145, 203, 204, 327, 463, 484
PspOMI GGGCCC 1 cut(s) 202
PspPI GGNCC 4 cut(s) 202, 203, 325, 419
PvuII CAGCTG 1 cut(s) 35
RsaI GTAC 3 cut(s) 10, 40, 484
RsaNI GTAC 3 cut(s) 9, 39, 483
SapI GCTCTTC 1 cut(s) 235
SaqAI TTAA 4 cut(s) 302, 308, 677, 694
SatI GCNGC 2 cut(s) 63, 603
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 4 cut(s) 202, 203, 325, 419
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 3 cut(s) 35, 211, 589
SfcI CTRYAG 1 cut(s) 360
SinI GGWCC 1 cut(s) 419
Sse9I AATT 3 cut(s) 275, 443, 615
SsiI CCGC 3 cut(s) 126, 286, 605
SspMI CTAG 1 cut(s) 140
StyI CCWWGG 3 cut(s) 66, 139, 541
TaaI ACNGT 2 cut(s) 364, 688
TaiI ACGT 1 cut(s) 264
TasI AATT 3 cut(s) 275, 443, 615
TfiI GAWTC 2 cut(s) 181, 469
Tru1I TTAA 4 cut(s) 302, 308, 677, 694
Tru9I TTAA 4 cut(s) 302, 308, 677, 694
TscAI CASTG 1 cut(s) 648
TseFI GTSAC 2 cut(s) 149, 628
TseI GCWGC 2 cut(s) 62, 602
Tsp45I GTSAC 2 cut(s) 149, 628
TspDTI ATGAA 2 cut(s) 557, 567
TspRI CASTG 1 cut(s) 648
Van91I CCANNNNNTGG 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 1 cut(s) 275
XmaJI CCTAGG 1 cut(s) 139
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.