MD03G1072100.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006627
Physical Location & Seq
Reverse (-)
5862327 .. 5867074
4748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1072100.v1.1.491

Sequence Viewer

Length: 2700 bp
ATGGAGGGTCTGTGGCGGGCGGCGACGGGCCAAGACCCGAGCCCGGAAGACTACACCGGAATCGAGTTCTGGTCCAATCCTGAGCGCGCCACGTGGCTGACCAAGCAAGGCGAGTACATCAAGACGTGGAGGCGCCGCTGGTTCGTCTTGAAACAGGGGAAGCTGTTCTGGTTCAAAGACAGTCACGTGACCCGCTCGTCGACGCCGCGCGGTGTGATCCCGGTCGGCACGTGCCTGACTGTGAAGGGCGCCGAGGACGTGGTCCACAAGCCCTGCGCCTTCGAGCTGTCGACTACCAACCACGACACGATGTACTTCATCGCCGATTCGGAGAAGGAGAAGGAGGAGTGGATTAACTCGATCGGACGGTCCATAGTTCAGCACTCCAGATCGCTCGCGGATTCCGAGGTCGTCGATTACGATAGCAGCCGACAAAAAAAACATCAAGTGAAAGCGGCTAGTGCGAGAGTGACCACAGAAGAGATGGCAACTCTAAGCACCCCCCTTATCTCTCTACCACGTCACCCACCAAACCCTCCATCGCCAACCGCCACTAACGAACTTTTCTCAAGCTGCCCACCTCTTTCCTCAATTGACCACTGCACCAACATCAAGCAGTTGAAGCAAGTCCACGCCCAAATGCTCCGTACAGGCCTTCTTTTCGACCCCTACTCCGCCAGCAAGCTTATCACCGTCTGTGCTCTCTCCTCCTTCTCCAGCCTCGACTATGCCCGCCAAGTGTTCGACCAAATTCCCCAACCAAATCTCTACTCTTGGAACACCCTCATTCGCGCGTATGCGTCGAGCACCGATCCGACTGAAAGCATCCTTGTATTTCTGGAAATGCTTTATCGCTGTATGGAAAGTCCCAATAAATTTACTTACCCGTTTGTGATCAAGGCGGCTTCGGAGCTCCGGGTTTTGGAGCTCGGAAGAGGGTTTCATGGCATGGTGATAAAGGCCTCGCTTGGTTCGGATGTTTATATTCTCAATTCTCTGGTTCATTTCTATGGATCGTGCGGGGATTTGGATTTGGCGCGGCGGGTTTTCGTGAAGACTCCTAAGAAAGATGTTGTGTCTTGGAATTCTATGATCACGGCTTTTGCGCACGGGAATTGTCCCGAGGAGGCGTTGGAGTTGTTTAAGGAGATGGAGGCAAAGAATGTGAAGCCGAATGATGTGACAATGGTGAGTGTGTTGTCAGCTTGCGCAAAGAAGGTTGATTTGGAGTTTGGGAGGTGGGTTTGTTCGCGTATCGAAAGGAATGAAATTGGAGAGAACTTGACTTTGAACAATGTTATGCTTGATATGTATGTGAAATGCGGGAGCATTGAGGAAGCGAAAAGATTGTTTGATAGGATGCCGGAGAAGGATATTGTCTCTTGGACTACAATACTTGATGGGTATGCTCAGTCAGGAAATTATGATGAGGCTTGGCAGATTTTCACTGCGATGCCTAGTCAAGACATTGCTGCATGGAATGTCCTCATTTCGTCTTATGAACAAAGTGGTAAGCCAAAGGAGGCTTTGGCGGTTTTTCATGACTTGCAGAAAAGGAAGAGCCCGAAACCTGATGAAGTCACTCTAGTTAGTACCCTGGCAGCCTGTGCTCAATTGGGAGCAATTGATCTTGGTGGCTGGATACATGTTTACGTAAAGAAGCAGGGTATGAAGATGAATTGTCACCTCACAACCTCGCTTATCGACATGTATGCAAAGTGTGGGGATCTGGATAAGGCACTTGAGGTGTTTAATTCAGTGGAGAGGAGAGATGTGTTCGTCTGGAGTGCCATGATTGCTGCTCTGGCAATGCATGGGAAAGGGAGGGAGGCATTGGATTGTTTCTCAAGAATGCTAGAAACCAAGGTGAAGCCCAATTCCGTGACATTCACCAACATGTTATGCGCTTGTAGCCACGCTGGATTGGTGGATGAAGGAAGAACATTTTTCTATCAAATGGAGCCAGTTTACGGGGTTGTGCCTGGTGTAAAGCACTATGCTTGCATGGTCGATATTTTAGGTCGTTCTGGAAATCTGGAGGAAGCTACAGAGTTGATAGAGAAAATGCCAATAGCTCCTACCGCGTCTGTATGGGGGGCTCTTCTTGGGGCCTGTGGACTTCATGGGAATGTCGAGCTTGCCGAAAAGGCGTGTAGCCATATGCTTGAGTTGGATCCTGGAAACCACGGAGCTTATGTACTCTTATCAAATATCTATGCCAAGACAGGGAAATGGGAAGAAGTTTCAGGGTTAAGGCAGCGTATGCGAGATGCAGGAATTAAGAAAGAACCCGGTTGTAGTGCAATCGAAGTCAATGGTATTGTTCATGAGTTTCTAGTTGGTGATAACTCTCACCCTCTGTGCAAAGAAATATATTCAAAGTTGGATGAAATAGCAGGGAGATTGAAGTCAAATGGATACGTGCCAAACAAGTCAAATCTACTGCAGTTTGTTGAGGAAGAAGACATGAAGAACCATGCCCTAATCCTTCACAGCGAGAAATTAGCGATTGCATTCGGGCTTATCAGCTTAAGTCCATCTCAACCAATTCAGGTTGTGAAGAATCTTCGTGTTTGCGGAGACTGCCACGCAGTTGCTAAGCTTATATCAAGGGTTTACGATAGGGAGATCTTGCTGAGAGATCGATATCGGTTTCATCATTTCAGAGACGGGCATTGCTCATGTAACGATTATTGGTGA

Protein Analysis

900

Amino Acids

100.84

Weight (kDa)

6.49

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 30 - 125 1.2e-18 PH domain
PPR_2 PF13041 356 - 405 2.8e-13 PPR repeat family
PPR PF01535 359 - 389 2.3e-08 PPR repeat
PPR_2 PF13041 457 - 488 3e-06 PPR repeat family
PPR_2 PF13041 489 - 537 4.1e-07 PPR repeat family
PPR_2 PF13041 590 - 638 9.5e-11 PPR repeat family
E_motif PF20431 708 - 770 8.8e-24 E motif
Eplus_motif PF20430 775 - 801 4.2e-08 E+ motif
DYW_deaminase PF14432 806 - 899 3e-35 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 196
Acc16I TGCGCA 2 cut(s) 1107, 1210
AccB1I GGYRCC 2 cut(s) 132, 248
AccBSI CCGCTC 1 cut(s) 195
AccI GTMKAC 2 cut(s) 200, 290
AccII CGCG 9 cut(s) 87, 208, 210, 398, 792, 794, 1039, 1252, 2084
AclWI GGATC 6 cut(s) 211, 806, 1021, 1734, 2168, 2181
AcsI RAATTY 3 cut(s) 750, 875, 1084
AcvI CACGTG 3 cut(s) 93, 187, 231
AcyI GRCGYC 3 cut(s) 133, 203, 249
AfaI GTAC 5 cut(s) 116, 314, 649, 1594, 2199
AfiI CCNNNNNNNGG 4 cut(s) 43, 922, 1970, 2226
AflII CTTAAG 1 cut(s) 2530
AflIII ACRYGT 3 cut(s) 1645, 1707, 1896
AgsI TTSAA 6 cut(s) 151, 175, 622, 1291, 2379, 2407
AjiI CACGTC 3 cut(s) 126, 259, 521
AjnI CCWGG 3 cut(s) 1596, 1981, 2176
AjuI GAANNNNNNNTTGG 2 cut(s) 1208, 1240
Alw21I GWGCWC 5 cut(s) 703, 809, 915, 930, 1612
Alw26I GTCTC 3 cut(s) 1384, 2574, 2661
AlwI GGATC 6 cut(s) 211, 806, 1021, 1734, 2168, 2181
Ama87I CYCGRG 2 cut(s) 37, 1121
AoxI GGCC 4 cut(s) 28, 652, 960, 2109
ApeKI GCWGC 6 cut(s) 426, 573, 1472, 1601, 1799, 2257
ApoI RAATTY 3 cut(s) 750, 875, 1084
Asp700I GAANNNNTTC 1 cut(s) 164
AspS9I GGNCC 5 cut(s) 28, 72, 262, 369, 2109
AsuC2I CCSGG 4 cut(s) 44, 221, 917, 2292
AsuHPI GGTGA 9 cut(s) 515, 682, 964, 1201, 1676, 1879, 1882, 2345, 2354
AvaI CYCGRG 2 cut(s) 37, 1121
AvaII GGWCC 3 cut(s) 72, 262, 369
BamHI GGATCC 1 cut(s) 2173
BanI GGYRCC 2 cut(s) 132, 248
BanII GRGCYC 5 cut(s) 44, 915, 930, 1565, 2101
BbrPI CACGTG 3 cut(s) 93, 187, 231
BbsI GAAGAC 3 cut(s) 54, 1061, 2469
Bbv12I GWGCWC 5 cut(s) 703, 809, 915, 930, 1612
BbvI GCAGC 6 cut(s) 438, 560, 1459, 1613, 1786, 2269
BccI CCATC 5 cut(s) 478, 547, 1144, 1394, 2545
BceAI ACGGC 1 cut(s) 1113
BcgI CGANNNNNNTGC 2 cut(s) 1694, 1728
BciT130I CCWGG 3 cut(s) 1598, 1983, 2178
BciVI GTATCC 2 cut(s) 1635, 2411
BclI TGATCA 2 cut(s) 894, 1092
BcnI CCSGG 4 cut(s) 44, 221, 917, 2292
BcoDI GTCTC 3 cut(s) 1384, 2574, 2661
BfaI CTAG 5 cut(s) 459, 1458, 1586, 1856, 2336
BfmI CTRYAG 2 cut(s) 2046, 2444
BfoI RGCGCY 2 cut(s) 136, 252
BfrI CTTAAG 1 cut(s) 2530
BfuI GTATCC 2 cut(s) 1635, 2411
BglI GCCNNNNNGGC 1 cut(s) 2147
BglII AGATCT 1 cut(s) 2628
BlpI GCTNAGC 1 cut(s) 2598
Bme1390I CCNGG 7 cut(s) 44, 221, 917, 1598, 1983, 2178, 2292
Bme18I GGWCC 3 cut(s) 72, 262, 369
BmeT110I CYCGRG 2 cut(s) 37, 1121
BmgBI CACGTC 3 cut(s) 126, 259, 521
BmgT120I GGNCC 5 cut(s) 28, 72, 262, 369, 2109
BmiI GGNNCC 5 cut(s) 134, 250, 1962, 2110, 2175
BmrFI CCNGG 7 cut(s) 44, 221, 917, 1598, 1983, 2178, 2292
BmsI GCATC 4 cut(s) 834, 1350, 1443, 2260
BpiI GAAGAC 3 cut(s) 54, 1061, 2469
BpmI CTGGAG 4 cut(s) 370, 700, 1804, 2057
Bpu10I CCTNAGC 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 2598
BpuEI CTTGAG 4 cut(s) 553, 1763, 1831, 2186
BpuMI CCSGG 4 cut(s) 44, 221, 917, 2292
Bsa29I ATCGAT 1 cut(s) 2644
BsaAI YACGTR 5 cut(s) 93, 187, 231, 1654, 2422
BsaBI GATNNNNATC 1 cut(s) 2646
BsaHI GRCGYC 3 cut(s) 133, 203, 249
BsaJI CCNNGG 6 cut(s) 252, 405, 1122, 1596, 1863, 2185
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 5 cut(s) 26, 656, 686, 2181, 2211
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 922, 1970, 2226
Bse1I ACTGG 1 cut(s) 1964
Bse3DI GCAATG 3 cut(s) 1467, 1815, 2674
Bse8I GATNNNNATC 1 cut(s) 2646
BseBI CCWGG 3 cut(s) 1598, 1983, 2178
BseCI ATCGAT 1 cut(s) 2644
BseDI CCNNGG 6 cut(s) 252, 405, 1122, 1596, 1863, 2185
BseGI GGATG 5 cut(s) 825, 982, 1365, 1936, 2392
BseJI GATNNNNATC 1 cut(s) 2646
BseLI CCNNNNNNNGG 4 cut(s) 43, 922, 1970, 2226
BseMI GCAATG 3 cut(s) 1467, 1815, 2674
BseMII CTCAG 3 cut(s) 72, 1424, 2627
BseNI ACTGG 1 cut(s) 1964
BsePI GCGCGC 1 cut(s) 85
BseRI GAGGAG 4 cut(s) 359, 697, 1139, 1780
BseXI GCAGC 6 cut(s) 438, 560, 1459, 1613, 1786, 2269
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 9 cut(s) 87, 208, 210, 398, 792, 794, 1039, 1252, 2084
Bsh1285I CGRYCG 2 cut(s) 225, 363
BshFI GGCC 4 cut(s) 30, 654, 962, 2111
BshNI GGYRCC 2 cut(s) 132, 248
BshVI ATCGAT 1 cut(s) 2644
BsiEI CGRYCG 2 cut(s) 225, 363
BsiHKAI GWGCWC 5 cut(s) 703, 809, 915, 930, 1612
BsiHKCI CYCGRG 2 cut(s) 37, 1121
BsiSI CCGG 6 cut(s) 44, 57, 221, 916, 1364, 2292
BslFI GGGAC 2 cut(s) 852, 1104
BslI CCNNNNNNNGG 4 cut(s) 43, 922, 1970, 2226
BsmAI GTCTC 3 cut(s) 1384, 2574, 2661
BsmBI CGTCTC 1 cut(s) 2661
BsmFI GGGAC 2 cut(s) 852, 1104
BsmI GAATGC 2 cut(s) 1857, 2513
BsnI GGCC 4 cut(s) 30, 654, 962, 2111
BsoBI CYCGRG 2 cut(s) 37, 1121
Bsp1286I GDGCHC 8 cut(s) 44, 703, 809, 915, 930, 1565, 1612, 2101
Bsp1720I GCTNAGC 1 cut(s) 2598
BspANI GGCC 4 cut(s) 30, 654, 962, 2111
BspCNI CTCAG 3 cut(s) 73, 1423, 2628
BspDI ATCGAT 1 cut(s) 2644
BspFNI CGCG 9 cut(s) 87, 208, 210, 398, 792, 794, 1039, 1252, 2084
BspHI TCATGA 2 cut(s) 1540, 2326
BspLI GGNNCC 5 cut(s) 134, 250, 1962, 2110, 2175
BspMAI CTGCAG 1 cut(s) 2448
BspPI GGATC 6 cut(s) 211, 806, 1021, 1734, 2168, 2181
BspQI GCTCTTC 2 cut(s) 1553, 2106
BspT107I GGYRCC 2 cut(s) 132, 248
BspTI CTTAAG 1 cut(s) 2530
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 3 cut(s) 1467, 1815, 2674
BsrI ACTGG 1 cut(s) 1964
BssECI CCNNGG 6 cut(s) 252, 405, 1122, 1596, 1863, 2185
BssHII GCGCGC 1 cut(s) 85
BssNI GRCGYC 3 cut(s) 133, 203, 249
BssT1I CCWWGG 1 cut(s) 1863
Bst2UI CCWGG 3 cut(s) 1598, 1983, 2178
Bst4CI ACNGT 4 cut(s) 182, 241, 369, 694
Bst6I CTCTTC 4 cut(s) 474, 928, 1553, 2106
BstACI GRCGYC 3 cut(s) 133, 203, 249
BstAFI CTTAAG 1 cut(s) 2530
BstAPI GCANNNNNTGC 2 cut(s) 1607, 2263
BstBAI YACGTR 5 cut(s) 93, 187, 231, 1654, 2422
BstC8I GCNNGC 9 cut(s) 18, 87, 396, 679, 683, 733, 1207, 2002, 2139
BstDEI CTNAG 6 cut(s) 81, 494, 1062, 1410, 2598, 2636
BstDSI CCRYGG 1 cut(s) 2185
BstF5I GGATG 5 cut(s) 825, 982, 1365, 1936, 2392
BstFNI CGCG 9 cut(s) 87, 208, 210, 398, 792, 794, 1039, 1252, 2084
BstH2I RGCGCY 2 cut(s) 136, 252
BstMAI GTCTC 3 cut(s) 1384, 2574, 2661
BstMCI CGRYCG 2 cut(s) 225, 363
BstNI CCWGG 3 cut(s) 1598, 1983, 2178
BstNSI RCATGY 3 cut(s) 1649, 1711, 1900
BstSCI CCNGG 7 cut(s) 42, 219, 915, 1596, 1981, 2176, 2290
BstSFI CTRYAG 2 cut(s) 2046, 2444
BstSNI TACGTA 1 cut(s) 1654
BstUI CGCG 9 cut(s) 87, 208, 210, 398, 792, 794, 1039, 1252, 2084
BstV1I GCAGC 6 cut(s) 438, 560, 1459, 1613, 1786, 2269
BstV2I GAAGAC 3 cut(s) 54, 1061, 2469
BstX2I RGATCY 3 cut(s) 1726, 2173, 2628
BstYI RGATCY 3 cut(s) 1726, 2173, 2628
Bsu15I ATCGAT 1 cut(s) 2644
BsuI GTATCC 2 cut(s) 1635, 2411
BsuRI GGCC 4 cut(s) 30, 654, 962, 2111
BsuTUI ATCGAT 1 cut(s) 2644
BtgI CCRYGG 1 cut(s) 2185
BtgZI GCGATG 3 cut(s) 304, 525, 1466
BtrI CACGTC 3 cut(s) 126, 259, 521
BtsCI GGATG 5 cut(s) 825, 982, 1365, 1936, 2392
BtsI GCAGTG 2 cut(s) 598, 1446
BtsIMutI CAGTG 3 cut(s) 598, 1446, 1764
Cac8I GCNNGC 9 cut(s) 18, 87, 396, 679, 683, 733, 1207, 2002, 2139
CciI TCATGA 2 cut(s) 1540, 2326
Cfr13I GGNCC 5 cut(s) 28, 72, 262, 369, 2109
ClaI ATCGAT 1 cut(s) 2644
CseI GACGC 3 cut(s) 211, 789, 2073
Csp6I GTAC 5 cut(s) 115, 313, 648, 1593, 2198
CviQI GTAC 5 cut(s) 115, 313, 648, 1593, 2198
DdeI CTNAG 6 cut(s) 81, 494, 1062, 1410, 2598, 2636
DinI GGCGCC 2 cut(s) 134, 250
DrdI GACNNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 1 cut(s) 196
Eam1104I CTCTTC 4 cut(s) 474, 928, 1553, 2106
EarI CTCTTC 4 cut(s) 474, 928, 1553, 2106
EciI GGCGGA 1 cut(s) 664
Ecl136II GAGCTC 2 cut(s) 913, 928
Eco105I TACGTA 1 cut(s) 1654
Eco130I CCWWGG 1 cut(s) 1863
Eco147I AGGCCT 2 cut(s) 654, 962
Eco24I GRGCYC 5 cut(s) 44, 915, 930, 1565, 2101
Eco32I GATATC 1 cut(s) 2648
Eco47I GGWCC 3 cut(s) 72, 262, 369
Eco53kI GAGCTC 2 cut(s) 913, 928
Eco72I CACGTG 3 cut(s) 93, 187, 231
Eco88I CYCGRG 2 cut(s) 37, 1121
EcoICRI GAGCTC 2 cut(s) 913, 928
EcoO109I RGGNCCY 1 cut(s) 2109
EcoRI GAATTC 1 cut(s) 1084
EcoRII CCWGG 3 cut(s) 1596, 1981, 2176
EcoRV GATATC 1 cut(s) 2648
EcoT14I CCWWGG 1 cut(s) 1863
EcoT22I ATGCAT 1 cut(s) 1815
EcoT38I GRGCYC 5 cut(s) 44, 915, 930, 1565, 2101
EgeI GGCGCC 2 cut(s) 134, 250
EheI GGCGCC 2 cut(s) 134, 250
ErhI CCWWGG 1 cut(s) 1863
Esp3I CGTCTC 1 cut(s) 2661
FaqI GGGAC 2 cut(s) 852, 1104
FauI CCCGC 6 cut(s) 9, 200, 740, 1013, 1035, 1316
FauNDI CATATG 1 cut(s) 2160
FbaI TGATCA 2 cut(s) 894, 1092
FblI GTMKAC 2 cut(s) 200, 290
FokI GGATG 5 cut(s) 812, 989, 1372, 1943, 2399
FriOI GRGCYC 5 cut(s) 44, 915, 930, 1565, 2101
FspBI CTAG 5 cut(s) 459, 1458, 1586, 1856, 2336
FspI TGCGCA 2 cut(s) 1107, 1210
GsuI CTGGAG 4 cut(s) 370, 700, 1804, 2057
HaeII RGCGCY 2 cut(s) 136, 252
HaeIII GGCC 4 cut(s) 30, 654, 962, 2111
HapII CCGG 6 cut(s) 44, 57, 221, 916, 1364, 2292
HgaI GACGC 3 cut(s) 211, 789, 2073
Hin1I GRCGYC 3 cut(s) 133, 203, 249
HincII GTYRAC 2 cut(s) 201, 291
HindII GTYRAC 2 cut(s) 201, 291
HindIII AAGCTT 2 cut(s) 683, 2600
HinfI GANTC 5 cut(s) 60, 326, 401, 1057, 2563
HpaII CCGG 6 cut(s) 44, 57, 221, 916, 1364, 2292
HphI GGTGA 9 cut(s) 515, 682, 964, 1201, 1676, 1879, 1882, 2345, 2354
Hpy166II GTNNAC 8 cut(s) 201, 265, 291, 631, 1651, 1969, 2117, 2617
Hpy188I TCNGA 8 cut(s) 331, 365, 406, 816, 910, 932, 976, 2666
Hpy8I GTNNAC 8 cut(s) 201, 265, 291, 631, 1651, 1969, 2117, 2617
Hpy99I CGWCG 5 cut(s) 28, 202, 205, 416, 805
HpyCH4III ACNGT 4 cut(s) 182, 241, 369, 694
HpyCH4IV ACGT 8 cut(s) 92, 125, 186, 230, 258, 520, 1653, 2421
HpyF3I CTNAG 6 cut(s) 81, 494, 1062, 1410, 2598, 2636
HpySE526I ACGT 8 cut(s) 92, 125, 186, 230, 258, 520, 1653, 2421
Hsp92I GRCGYC 3 cut(s) 133, 203, 249
KasI GGCGCC 2 cut(s) 132, 248
Ksp22I TGATCA 2 cut(s) 894, 1092
LguI GCTCTTC 2 cut(s) 1553, 2106
LmnI GCTCC 9 cut(s) 648, 910, 918, 925, 1326, 1619, 1960, 2080, 2189
Lsp1109I GCAGC 6 cut(s) 438, 560, 1459, 1613, 1786, 2269
LweI GCATC 4 cut(s) 834, 1350, 1443, 2260
MaeI CTAG 5 cut(s) 459, 1458, 1586, 1856, 2336
MaeII ACGT 8 cut(s) 92, 125, 186, 230, 258, 520, 1653, 2421
MaeIII GTNAC 9 cut(s) 182, 187, 469, 521, 1180, 1579, 1682, 1882, 2684
MbiI CCGCTC 1 cut(s) 195
MfeI CAATTG 3 cut(s) 591, 1613, 1623
MflI RGATCY 3 cut(s) 1726, 2173, 2628
MhlI GDGCHC 8 cut(s) 44, 703, 809, 915, 930, 1565, 1612, 2101
Mly113I GGCGCC 2 cut(s) 133, 249
MlyI GAGTC 1 cut(s) 1051
MmeI TCCRAC 4 cut(s) 839, 1113, 2151, 2364
Mph1103I ATGCAT 1 cut(s) 1815
MroXI GAANNNNTTC 1 cut(s) 164
MseI TTAA 6 cut(s) 354, 1143, 1752, 2252, 2280, 2531
MslI CAYNNNNRTG 4 cut(s) 1008, 1451, 1895, 2127
MspA1I CMGCKG 1 cut(s) 138
MspCI CTTAAG 1 cut(s) 2530
MspI CCGG 6 cut(s) 44, 57, 221, 916, 1364, 2292
MspR9I CCNGG 7 cut(s) 44, 221, 917, 1598, 1983, 2178, 2292
MunI CAATTG 3 cut(s) 591, 1613, 1623
Mva1269I GAATGC 2 cut(s) 1857, 2513
MvaI CCWGG 3 cut(s) 1598, 1983, 2178
MvnI CGCG 9 cut(s) 87, 208, 210, 398, 792, 794, 1039, 1252, 2084
NarI GGCGCC 2 cut(s) 133, 249
NciI CCSGG 4 cut(s) 44, 221, 917, 2292
NdeI CATATG 1 cut(s) 2160
NlaIV GGNNCC 5 cut(s) 134, 250, 1962, 2110, 2175
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 8 cut(s) 182, 187, 469, 521, 1180, 1579, 1682, 1882
NsbI TGCGCA 2 cut(s) 1107, 1210
NsiI ATGCAT 1 cut(s) 1815
NspI RCATGY 3 cut(s) 1649, 1711, 1900
PagI TCATGA 2 cut(s) 1540, 2326
PauI GCGCGC 1 cut(s) 85
PceI AGGCCT 2 cut(s) 654, 962
PciI ACATGT 3 cut(s) 1645, 1707, 1896
PciSI GCTCTTC 2 cut(s) 1553, 2106
PcsI WCGNNNNNNNCGW 3 cut(s) 402, 417, 2139
PctI GAATGC 2 cut(s) 1857, 2513
PdmI GAANNNNTTC 1 cut(s) 164
PfeI GAWTC 4 cut(s) 60, 326, 401, 2563
PflFI GACNNNGTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 2176
Ple19I CGATCG 1 cut(s) 363
PleI GAGTC 1 cut(s) 1051
PluTI GGCGCC 2 cut(s) 136, 252
PmaCI CACGTG 3 cut(s) 93, 187, 231
PmlI CACGTG 3 cut(s) 93, 187, 231
PpsI GAGTC 1 cut(s) 1051
Ppu21I YACGTR 5 cut(s) 93, 187, 231, 1654, 2422
PscI ACATGT 3 cut(s) 1645, 1707, 1896
Psp124BI GAGCTC 2 cut(s) 915, 930
Psp6I CCWGG 3 cut(s) 1596, 1981, 2176
PspCI CACGTG 3 cut(s) 93, 187, 231
PspGI CCWGG 3 cut(s) 1596, 1981, 2176
PspN4I GGNNCC 5 cut(s) 134, 250, 1962, 2110, 2175
PspPI GGNCC 5 cut(s) 28, 72, 262, 369, 2109
PstI CTGCAG 1 cut(s) 2448
PsuI RGATCY 3 cut(s) 1726, 2173, 2628
PsyI GACNNNGTC 1 cut(s) 260
PteI GCGCGC 1 cut(s) 85
PvuI CGATCG 1 cut(s) 363
RsaI GTAC 5 cut(s) 116, 314, 649, 1594, 2199
RsaNI GTAC 5 cut(s) 115, 313, 648, 1593, 2198
RseI CAYNNNNRTG 4 cut(s) 1008, 1451, 1895, 2127
SacI GAGCTC 2 cut(s) 915, 930
SalI GTCGAC 2 cut(s) 199, 289
SapI GCTCTTC 2 cut(s) 1553, 2106
SaqAI TTAA 6 cut(s) 354, 1143, 1752, 2252, 2280, 2531
Sau96I GGNCC 5 cut(s) 28, 72, 262, 369, 2109
SchI GAGTC 1 cut(s) 1051
ScrFI CCNGG 7 cut(s) 44, 221, 917, 1598, 1983, 2178, 2292
SduI GDGCHC 8 cut(s) 44, 703, 809, 915, 930, 1565, 1612, 2101
SfaNI GCATC 4 cut(s) 834, 1350, 1443, 2260
SfcI CTRYAG 2 cut(s) 2046, 2444
SfoI GGCGCC 2 cut(s) 134, 250
SgrDI CGTCGACG 1 cut(s) 199
SinI GGWCC 3 cut(s) 72, 262, 369
SmiMI CAYNNNNRTG 4 cut(s) 1008, 1451, 1895, 2127
SmlI CTYRAG 5 cut(s) 568, 1742, 1846, 2165, 2530
SmoI CTYRAG 5 cut(s) 568, 1742, 1846, 2165, 2530
SnaBI TACGTA 1 cut(s) 1654
SseBI AGGCCT 2 cut(s) 654, 962
SspDI GGCGCC 2 cut(s) 132, 248
SspMI CTAG 5 cut(s) 459, 1458, 1586, 1856, 2336
SstI GAGCTC 2 cut(s) 915, 930
StuI AGGCCT 2 cut(s) 654, 962
StyD4I CCNGG 7 cut(s) 42, 219, 915, 1596, 1981, 2176, 2290
StyI CCWWGG 1 cut(s) 1863
TaaI ACNGT 4 cut(s) 182, 241, 369, 694
TaiI ACGT 8 cut(s) 95, 128, 189, 233, 261, 523, 1656, 2424
TatI WGTACW 3 cut(s) 114, 312, 2197
TauI GCSGC 6 cut(s) 23, 138, 208, 458, 905, 1042
TfiI GAWTC 4 cut(s) 60, 326, 401, 2563
Tru1I TTAA 6 cut(s) 354, 1143, 1752, 2252, 2280, 2531
Tru9I TTAA 6 cut(s) 354, 1143, 1752, 2252, 2280, 2531
TscAI CASTG 3 cut(s) 605, 1453, 1764
TseFI GTSAC 8 cut(s) 182, 187, 469, 521, 1180, 1579, 1682, 1882
TseI GCWGC 6 cut(s) 426, 573, 1472, 1601, 1799, 2257
Tsp45I GTSAC 8 cut(s) 182, 187, 469, 521, 1180, 1579, 1682, 1882
TspGWI ACGGA 3 cut(s) 635, 1870, 2202
TspRI CASTG 3 cut(s) 605, 1453, 1764
Tth111I GACNNNGTC 1 cut(s) 260
Vha464I CTTAAG 1 cut(s) 2530
VpaK11BI GGWCC 3 cut(s) 72, 262, 369
XapI RAATTY 3 cut(s) 750, 875, 1084
XceI RCATGY 3 cut(s) 1649, 1711, 1900
XcmI CCANNNNNNNNNTGG 1 cut(s) 481
XmiI GTMKAC 2 cut(s) 200, 290
XmnI GAANNNNTTC 1 cut(s) 164
XspI CTAG 5 cut(s) 459, 1458, 1586, 1856, 2336
Zsp2I ATGCAT 1 cut(s) 1815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.