Rh5AG452500

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006627
Physical Location & Seq
Forward (+)
77565928 .. 77570549
4622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG452500.1

Sequence Viewer

Length: 531 bp
ATGCTTGACGAGTGCCCCAACAAGTTCACTTTCCCTTTTCTACAAAAGGCGGTGTCGAAGCTCCAGGATTCCAAGGTTGGGAGAGGTTTTCATGCCATGGTGATAAAAGCCAACCTGTGTTCGGATGTTTATATTATTAATTCGCTCATTCACTTTTATGGGTCTTTTTGGGATTTGAATTTGGCGCGTTTGGTGTTTTTGAAGACTTATAAGAAGGATGTTGTTACTTGGAATTCTATTATCACGGCTTTTGCTCAGGGAAATTTCCTTGAGGTGGCGTTGGTGTTGTTTAAGGAAATAGAGGCGGAGAATATGAAGCCAAATAATGTGATATTGGCGTTTTGTGGCTTGTTGCTGGCGAGGGACTTGGTTGTCGATCTAGGTTACGGTAGATCGAGGCCTGTTGGGCAGACAGAGGTGGGGAGATTTGGGTGTGTAGTGAAGGCGGCTTATGCTGCAGGTGCAGGGGCTGAGAAGAGGAGGACGGGGATGGTGGAGCAGAGGCCAAGAGGTCAGATGACGTCGAATTAG

Protein Analysis

176

Amino Acids

19.62

Weight (kDa)

9.36

Isoelectric Point (pI)

32.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 72 - 110 1.6e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
AarI CACCTGC 1 cut(s) 449
AasI GACNNNNNNGTC 1 cut(s) 371
AatII GACGTC 1 cut(s) 524
Acc36I ACCTGC 1 cut(s) 449
AccII CGCG 1 cut(s) 187
AciI CCGC 3 cut(s) 50, 305, 446
AcsI RAATTY 3 cut(s) 178, 232, 262
AcyI GRCGYC 1 cut(s) 521
AfiI CCNNNNNNNGG 3 cut(s) 78, 121, 274
AgsI TTSAA 2 cut(s) 178, 202
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
AlwNI CAGNNNCTG 1 cut(s) 470
AoxI GGCC 2 cut(s) 398, 503
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 3 cut(s) 178, 232, 262
AseI ATTAAT 1 cut(s) 138
AspLEI GCGC 1 cut(s) 187
AsuHPI GGTGA 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 442
BccI CCATC 1 cut(s) 484
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 1 cut(s) 65
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 449
BglI GCCNNNNNGGC 1 cut(s) 406
BisI GCNGC 2 cut(s) 447, 456
BlsI GCNGC 2 cut(s) 448, 457
Bme1390I CCNGG 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 47
Bpu10I CCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 290
BsaHI GRCGYC 1 cut(s) 521
BsaJI CCNNGG 2 cut(s) 72, 96
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 121, 274
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 72, 96
BseGI GGATG 3 cut(s) 130, 223, 495
BseLI CCNNNNNNNGG 3 cut(s) 78, 121, 274
BseMII CTCAG 2 cut(s) 269, 462
BseRI GAGGAG 1 cut(s) 493
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 1 cut(s) 442
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 400, 505
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 3 cut(s) 78, 121, 274
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 2 cut(s) 400, 505
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 376, 392
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 3 cut(s) 50, 305, 446
BspANI GGCC 2 cut(s) 400, 505
BspCNI CTCAG 2 cut(s) 268, 463
BspFNI CGCG 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 72, 96
BssMI GATC 2 cut(s) 376, 392
BssNI GRCGYC 1 cut(s) 521
BssT1I CCWWGG 2 cut(s) 72, 96
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 389
Bst6I CTCTTC 1 cut(s) 470
BstACI GRCGYC 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 357
BstDEI CTNAG 2 cut(s) 255, 471
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 3 cut(s) 130, 223, 495
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 2 cut(s) 379, 395
BstMBI GATC 2 cut(s) 376, 392
BstMWI GCNNNNNNNGC 4 cut(s) 406, 452, 455, 461
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 442
BstV2I GAAGAC 1 cut(s) 209
BsuRI GGCC 2 cut(s) 400, 505
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 3 cut(s) 130, 223, 495
BveI ACCTGC 1 cut(s) 449
Cac8I GCNNGC 1 cut(s) 357
CaiI CAGNNNCTG 1 cut(s) 470
CfoI GCGC 1 cut(s) 187
CviAII CATG 2 cut(s) 92, 97
CviJI RGCY 9 cut(s) 61, 110, 248, 319, 348, 400, 449, 470, 505
CviKI_1 RGCY 9 cut(s) 61, 110, 248, 319, 348, 400, 449, 470, 505
DdeI CTNAG 2 cut(s) 255, 471
DpnI GATC 2 cut(s) 378, 394
DpnII GATC 2 cut(s) 376, 392
DrdI GACNNNNNNGTC 1 cut(s) 371
DseDI GACNNNNNNGTC 1 cut(s) 371
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 1 cut(s) 320
Eco130I CCWWGG 2 cut(s) 72, 96
Eco147I AGGCCT 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 2 cut(s) 72, 96
ErhI CCWWGG 2 cut(s) 72, 96
FaeI CATG 2 cut(s) 95, 100
FaiI YATR 7 cut(s) 93, 98, 132, 159, 210, 314, 453
FaqI GGGAC 1 cut(s) 377
FatI CATG 2 cut(s) 91, 96
Fnu4HI GCNGC 2 cut(s) 447, 456
FokI GGATG 3 cut(s) 137, 230, 502
Fsp4HI GCNGC 2 cut(s) 447, 456
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 186
GluI GCNGC 2 cut(s) 447, 456
GsuI CTGGAG 1 cut(s) 47
HaeIII GGCC 2 cut(s) 400, 505
HhaI GCGC 1 cut(s) 187
Hin1I GRCGYC 1 cut(s) 521
Hin1II CATG 2 cut(s) 95, 100
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HinfI GANTC 1 cut(s) 68
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 2 cut(s) 124, 516
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 2 cut(s) 208, 436
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4IV ACGT 1 cut(s) 521
HpyCH4V TGCA 2 cut(s) 458, 464
HpyF10VI GCNNNNNNNGC 4 cut(s) 406, 452, 455, 461
HpyF3I CTNAG 2 cut(s) 255, 471
HpySE526I ACGT 1 cut(s) 521
Hsp92I GRCGYC 1 cut(s) 521
Hsp92II CATG 2 cut(s) 95, 100
HspAI GCGC 1 cut(s) 185
Kzo9I GATC 2 cut(s) 376, 392
LmnI GCTCC 2 cut(s) 66, 496
LpnPI CCDG 8 cut(s) 50, 77, 128, 242, 341, 414, 444, 450
Lsp1109I GCAGC 1 cut(s) 442
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 521
MaeIII GTNAC 2 cut(s) 223, 383
MalI GATC 2 cut(s) 378, 394
MboI GATC 2 cut(s) 376, 392
MboII GAAGA 2 cut(s) 214, 487
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 5 cut(s) 139, 178, 232, 262, 526
MseI TTAA 2 cut(s) 138, 291
MslI CAYNNNNRTG 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 4 cut(s) 406, 452, 455, 461
NcoI CCATGG 1 cut(s) 96
NdeII GATC 2 cut(s) 376, 392
NlaIII CATG 2 cut(s) 95, 100
PaqCI CACCTGC 1 cut(s) 449
PceI AGGCCT 1 cut(s) 400
PfeI GAWTC 1 cut(s) 68
PfoI TCCNGGA 1 cut(s) 63
PkrI GCNGC 2 cut(s) 448, 457
PshBI ATTAAT 1 cut(s) 138
PsiI TTATAA 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 470
RseI CAYNNNNRTG 1 cut(s) 156
SaqAI TTAA 2 cut(s) 138, 291
SatI GCNGC 2 cut(s) 447, 456
Sau3AI GATC 2 cut(s) 376, 392
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 1 cut(s) 17
SfcI CTRYAG 1 cut(s) 456
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 5 cut(s) 139, 178, 232, 262, 526
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 3 cut(s) 50, 305, 446
SspMI CTAG 1 cut(s) 380
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 2 cut(s) 72, 96
TaaI ACNGT 1 cut(s) 389
TaiI ACGT 1 cut(s) 524
TaqI TCGA 4 cut(s) 56, 375, 395, 524
TasI AATT 5 cut(s) 139, 178, 232, 262, 526
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 138, 291
Tru9I TTAA 2 cut(s) 138, 291
TseI GCWGC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 80, 329
VspI ATTAAT 1 cut(s) 138
XapI RAATTY 3 cut(s) 178, 232, 262
XspI CTAG 1 cut(s) 380
ZraI GACGTC 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.