Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0006627

Physical Location & Seq

Genomic Coordinates Forward (+)

54302141 .. 54307838

Gene Structure 5698 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0395900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4653 bp

ATGAATCATACTCGACACCCGGGTCAGTCAAATCTAAGGGAAGGAGCAACTAACTTCATTCATAACCAAAAGGAGATAAGTTTGGTTTATGTTCAAATAGCGTTGTTGAACGTTAAACTATATGCTAAAAGGATTAGTGATATTATTATTTGTCTGTGCCATGAGGAGTTAGAAAAGTGTTATTCTGTAAGAGAAAGTAGCATGGAGTTCTTTACTGCAATTATTTCTGAGCTCGTTGGCATAATTGCTGACTATACCATTGAACCAATTGGACGCCAAGTGGGTTATCTGATATACTACAACAGCCACCTTCAAAATCTCAGAAGTCGAGTGGAGAACTTAAATGCTGCCAAAGACAGGATGCAGCATACCATTGATGAAGTTAAAAGAAAAGGTAAAAGGGTTGAAGCCGATGTTGAGAAGTGGCTGCAAACTGTGGATTTGATCAGTGTAGAGGTATATACATTCTTGGAAGATGAACGCCACGTAAAGAACAAGTACCTTCTTGGGTTATTTCCCAATCCCATTCTTTATTACCAGATAAGCAAGGAATCAGCAGAATTGGCACAGAAGGTTAAGCTCTATGAGAAAAGAGTATTTCCAAGTGTTTCATACAATGTTTCCCCTCAAGAGATATGTGGCATTCCTTCCCAAGAGTACCTGGCCTTTGACTCGAGGATTTCAGTTGTGAAGAAAATCATGGAGGAACTGAGAAATCCTGATACCAACATGATTGGGGTGTATGGTATTGGTGGAGTGGGGAAGACCACACTTTCCAAAGAGGTTTTTAGACAAGCTACAAAAGAGAAGTTATTTGATGATGTTGTGATAGTACTAGATGTGAATCATAAACCAGACCTCAAAAGAATTCAAAAAGAAATTGCTGAAAAGTTGGGTATGGATGTCCTTGAAAGTCAAACTATAGCAGGAAGAGCATGTCATATATGTGACAGGATTAAAGACAAGAGGATTCTTGTGATATTGGATGATGTGTGGGGAAGACTTGATTTGGAAGCTTTGGGGCTTCCCCGTGTAGCAACTTGTAATATATTGTTGACGTCTAGAAGTCGAGAAGTTTTGTCCTCCGAGATGCATACGCAAAAAGAGTTTCGACTTGAAGTCTTGAGTGAGGAAGAAACTTGGAGTTTGTTTGAAAAGATGGCAGGTGGTGTTGTTAAAGATGATCCCATACTGAAAATAGCAGCCCAAGTAGCCAAAAATTGTGGTGGTTTGCCGATTTTAGTTGTTACAGTTGCAAGTGCATTAAGAAATAGTGCTTTAGATGCATGGAAAGATGCCTTGAGACGATTAAAAAGGTTTGAAAAAAAGGGATTGACAGAAAAAGGATACTTGGCCCTAGAGTGGAGTTACAATCAGTTGGATGAAGAGGAGCTTAAGCCGTTGTTCTTGCTTTGTGGAATTATGGCAAATAATGACTACAATATTTCTCTTGGTGATATGTTGAAATATACGATGGGTTTGGGTTTGATTAAAAACGTTGACACAGTGGAAGAAGCACAGAATGCATTGCATTCACTGGTCAAAAGACTTAAAGATTCTTGTTTGTTGCTAGACGGTTCTTATACAAGTGCTTTTATTAATCCATGTGTCAGAATGCATGAACTCGTACGCAATGTTGCAATATGGATTGCATCTAGAGACCAACATGTCTTACTAAGAACAAATCAAGATCAGTTGAAAGAATGGCCAAATCAGGATTTCTTTGAGAAGTGCACTATGATCTATTTCCATTTCTGCAATATCCCCATGCTTCCTGAAGTACCTTGGAAATGTCGAGAAATAAAAATGTTTTATTTGGGTAGTAATCATGGCTCGCTAAAAATCCCATGCAACTTTTTTGAAGAGATGAAAGCACTCAAGGTGTTGGATTTAACTGGACTCTGCATCCCATCGATACCTCCATCTCTTCAATTCTTAACAAATCTTCGAACACTGTGTCTAGATCGGTGTGTGTTGGGCGATATTGCTCTAGTTGGACAGCTACGAAACCTTGAAATTCTTAGCTTTTTTAGATCTCAGATCAAACAGGTCCCAAAAGAAATAGGGAATTTGACTCAACTGAAGTTGTTGGAGTTCACCAGTTGCTCTGAACTTGTACTGATTTCTCCTGATGTTATATCAAGCTTGAAAAGACTTGAAGCTTTAAGAATGGGAATGGACAGCTTTAACCGATGGGAGAATGAAGGTGTCATCAGAAGTCAAACAAGGAGTAATGCTAGCCTTTCAGAGCTGAAGAACTTGTCCCGGCTAACCGCATTAGACATACATATTCTGGATGCTAACATTCTTCCACCAAACTTGTTCCCTCAGTTAGAAAGGTTCCACATACTTATTGGTGAAGCATGGGGATGGAATGATTTTGATAAACCCTTCAAGACACTAAAACTCAAGCTCACTACAAGCGATCAGTTGGACAAAGGTCTTAAAATGCTGCTGAGGAGTTCTGAACACTTATACTTGGATATAATGGAGGGTGTTAACGATATTTTTCATCTATTAGATGGTAATGGTTGTCAGCAATTAAAACATCTCCATGTGCAAAACAATGCTGAAGTTACATCTATTATCAAGTTGAATGCCTTCCCCACCTTGGAGTCACTATGTCTTTACAACCTTGCTAGCTTGGAAAGTGTATGTTATGGCCCAGCAATCGCTGAACATTTCCAAAAGCTACAGTCATTGACACTGTTGAGTCTGCCAAAGCTTGTCAGTTTCCTCTCCAAAGGTGGGACATCTATTGTAACTACAACAATGGGAGAGGTTGTAATGTCCAACTTAACAACCTTGATTGTGCATGGATGTGATAGTTTAGGATTCTTGTTTTCATCTTCTATGGCCAAAAGTCTTGTGCAACTTAAATATCTTGAGATATCCAGTTGTCTAATCATGAAAGAGGTAGTATCATCAAAAGAATCTGGTGCACAATATATGGAAAACATCTTTTATAAGTTAAACCATCTACAGCTACAGCATCTACCAAACCTCACAAGATTTTGCTCAGGAAGTTATATTGAACTTCAGTCCTTGGAGACATTGCATATAGAATATTGTACTAAACTGGAGAGCTTCATCTATGATCCTAGGATCAGGAAAGAAAGGGACTCGGAGAAGGATCTCGAGACTTCTGTGGAATACTTTCTTTTTGACAATAAGGTGGGACTTCCAAGCTTGGAGAGATTGATCCTCTATGACATACCTATGTTGGTGACAATATGGCACAACCAACTTGCTCCAGAATCTTTTATCAGACTCCGAAAAGTTAACATTTACAGATGCGACAGTTTAATAAATATCTTTGGGGATAGTATTATGCGAAGATTGACTGCTCTAGACACTTTGGAGATATGGCATTGTAGGTCGCTACAAGTGGTTTTTGAAGTTGGAGGAATCAACAGTAAAGAAACATTTGACACATCAACCACTCAGTCAAAAATATTTGAGTGCTGTCAAAATCTGGACTTAGTAGACATCGATTCATGTGAGAGTTTGAAAAGTATGTTTCCAAGCTCAGTAGCCAAAGGTCTTAAACAGCTGAGGATGCTGAGTGTGGATAATTGTGGTTTGGTGGAAATCATTGCAGAGGAAGGACTCGAAACAATGGCTGAGTTTGTGTTCCCAAAAGTAACATCTGTGATATTTAGAGATCTGCTCAAGCTTAGGAGTTTCTACCCAGGAATGCATTCTTGCAGGTGGCCATTACTTGAAGATCTTAAATTTGTGAGATGTCATAATATGGAGATCTTTGCCTCAGGATTTTCTAGTCAGGAAGAACTTGAGTTGGGACATATAATTGCTAAGGATTCATTCCCCAACTTGGAAAACTTGACCTTATGGACACAAGCAGACGTGGAGATTTGGTGTGATCTACTCCCAGCAATGTTTTTTAGCAAACTCAAATATCTTGTATATTATTCTGCAAAATCCTGGCCAGCTGCTTTCCTTACGAAATTAGTCAATCTTGAAAGGCTTGAACTTTATGGCCCTGCACAAGAAATATTTGTCAATGAAGGAAATACTAGTGCGGAAATACATGCAATTGGAACCCAGCTCCCACATTTAAGATCCCTGGAGCTTATAAGAATGCCCATGCTGATGCATCTAGGGGAGCAGAATTCCCAATCAGCAGGCCCAATTTTTCCGAACCTAAAAATTCTAGATGTGTGGCTCTGTGGGAAGTTGAAGAATTTAAGGTCATCTGCCATAACCTTTCAGAATTTAGCAACACTGAAAGTAGCTCGCTGCCATGGACTGGAATATTTGATAACTTCCTCAATGGCCAGGACTTTTGTGCAACTCAAAAACATGGAAGTCAAAGATTGCTCAAGACTGATTGAAATAGTGGCATTTAGCAACGATGATGATGCAGGAAATGACGTATCTTTCAGCCGGTTGCAACATTTAGAACTTTCTGATTTACCAAGTCTGCGAAGATTTTGTGGGGGCGATTGCAGTGTCAACTTGCCATCTTTAGACACTTTAACCATAACTGGCTGCCCTGTCATGTTGACAATTTCTCCTGAAGGTGTACTTCTAAGTGATTCAAAACCAGAAAGAGTAGAGCGGAAGAGGAAGAGGAATGACTATGAGGATCAAACGGAAGGGTCCGATAACACATCAAGTCTTGCCACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR

Protein Analysis

1550

Amino Acids

176.37

Weight (kDa)

6.02

Isoelectric Point (pI)

48.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NB-ARC	PF00931	230 - 388	7.6e-32	NB-ARC domain
LRR_14	PF23598	586 - 885	9.8e-11	Leucine-rich repeat region
LRR_RPS2	PF23247	842 - 967	3.1e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	928 - 1030	2e-15	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	984 - 1126	7.1e-17	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1065 - 1146	1.1e-11	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1155 - 1194	5.8e-10	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1281 - 1406	6.3e-09	Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2	PF23247	1405 - 1511	8.9e-13	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006627)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G29760
fragaria_vesca	FvH4_3g38420
malus_domestica	MD03G1072100.v1.1 MD11G1076100.v1.1 MD11G1076300.v1.1 MD11G1076700.v1.1
prunus_persica	Prupe.6G058800_v2.0.a1
pyrus_communis	pycom03g05830 pycom12g05280
rosa_chinensis	RchiOBHm_Chr5g0069041 RchiOBHm_Chr5g0069081
rosa_laevigata	RLG00000017743 RLG00000036033
rosa_multiflora	Rmu_co8508453.1_g000001 Rmu_sc0000853.1_g000010 Rmu_sc0000853.1_g000011 Rmu_sc0000936.1_g000009
rosa_roxburghii	Rroxscaffold_1G00011680
rosa_rugosa	Rorug05G0395900
rosa_samantha	Rh5AG452500 Rh5AG482500 Rh5BG471800 Rh5BG503600 Rh5CG494200
rosa_wichuraiana	Rw0G006080 Rw5G042280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2967, 4097
AarI	CACCTGC	2 cut(s)	1154, 3699
AasI	GACNNNNNNGTC	2 cut(s)	21, 1667
AatII	GACGTC	1 cut(s)	1061
Acc36I	ACCTGC	2 cut(s)	1154, 3699
AccB7I	CCANNNNNTGG	1 cut(s)	4270
AccBSI	CCGCTC	1 cut(s)	4582
AccI	GTMKAC	1 cut(s)	3486
AciI	CCGC	3 cut(s)	2274, 4043, 4582
AclI	AACGTT	2 cut(s)	111, 1497
AclWI	GGATC	7 cut(s)	1178, 3092, 3113, 3141, 3196, 4077, 4617
AcoI	YGGCCR	5 cut(s)	1706, 2856, 3713, 3947, 4296
AcsI	RAATTY	8 cut(s)	867, 2016, 2068, 3734, 4132, 4170, 4204, 4234
AcuI	CTGAAG	6 cut(s)	1797, 2102, 2273, 2592, 3023, 4560
AcyI	GRCGYC	2 cut(s)	274, 1058
AfaI	GTAC	8 cut(s)	500, 659, 834, 1629, 1782, 2118, 3073, 4548
AfiI	CCNNNNNNNGG	6 cut(s)	357, 1362, 2611, 2748, 3835, 4270
AflII	CTTAAG	1 cut(s)	1394
AflIII	ACRYGT	1 cut(s)	1666
AhdI	GACNNNNNGTC	1 cut(s)	1118
AhlI	ACTAGT	1 cut(s)	4037
AjiI	CACGTC	1 cut(s)	3868
AjnI	CCWGG	5 cut(s)	660, 3691, 3944, 4086, 4298
Alw21I	GWGCWC	3 cut(s)	234, 1736, 2944
Alw26I	GTCTC	4 cut(s)	1297, 1653, 3044, 3134
Alw44I	GTGCAC	2 cut(s)	1732, 2940
AlwI	GGATC	7 cut(s)	1178, 3092, 3113, 3141, 3196, 4077, 4617
Ama87I	CYCGRG	3 cut(s)	19, 673, 3137
ApaLI	GTGCAC	2 cut(s)	1732, 2940
ApeKI	GCWGC	8 cut(s)	347, 364, 427, 1202, 2452, 3953, 4260, 4512
ApoI	RAATTY	8 cut(s)	867, 2016, 2068, 3734, 4132, 4170, 4204, 4234
ArsI	GACNNNNNNTTYG	2 cut(s)	1942, 1974
AseI	ATTAAT	1 cut(s)	1599
Asp700I	GAANNNNTTC	3 cut(s)	2600, 3156, 3700
AspA2I	CCTAGG	1 cut(s)	3101
AspS9I	GGNCC	6 cut(s)	1354, 2050, 2663, 4001, 4148, 4623
AsuC2I	CCSGG	3 cut(s)	20, 21, 2266
AsuHPI	GGTGA	4 cut(s)	1466, 2089, 2369, 3238
AsuII	TTCGAA	1 cut(s)	1948
AsuNHI	GCTAGC	2 cut(s)	2237, 2639
AvaI	CYCGRG	3 cut(s)	19, 673, 3137
AvaII	GGWCC	2 cut(s)	2050, 4623
AvrII	CCTAGG	1 cut(s)	3101
AxyI	CCTNAGG	1 cut(s)	3769
BaeGI	GKGCMC	2 cut(s)	1736, 2944
BalI	TGGCCA	5 cut(s)	1708, 2858, 3715, 3949, 4298
BanII	GRGCYC	1 cut(s)	234
BbsI	GAAGAC	2 cut(s)	770, 1006
Bbv12I	GWGCWC	3 cut(s)	234, 1736, 2944
BbvCI	CCTCAGC	2 cut(s)	2456, 3554
BbvI	GCAGC	8 cut(s)	334, 376, 414, 1214, 2439, 3940, 4247, 4499
BccI	CCATC	9 cut(s)	1153, 1468, 1918, 1930, 2187, 2364, 2516, 2985, 4492
BceAI	ACGGC	1 cut(s)	1384
BcgI	CGANNNNNNTGC	2 cut(s)	4364, 4398
BciT130I	CCWGG	5 cut(s)	662, 3693, 3946, 4088, 4300
BciVI	GTATCC	1 cut(s)	1340
BclI	TGATCA	1 cut(s)	444
BcnI	CCSGG	3 cut(s)	20, 21, 2266
BcoDI	GTCTC	4 cut(s)	1297, 1653, 3044, 3134
BcuI	ACTAGT	1 cut(s)	4037
BfmI	CTRYAG	4 cut(s)	921, 2693, 2981, 2987
BfrI	CTTAAG	1 cut(s)	1394
BfuAI	ACCTGC	2 cut(s)	1154, 3699
BfuI	GTATCC	1 cut(s)	1340
BglII	AGATCT	4 cut(s)	2033, 3664, 3727, 3759
BisI	GCNGC	8 cut(s)	348, 365, 428, 1203, 2453, 3954, 4261, 4513
BlnI	CCTAGG	1 cut(s)	3101
BlsI	GCNGC	8 cut(s)	349, 366, 429, 1204, 2454, 3955, 4262, 4514
BmcAI	AGTACT	1 cut(s)	834
Bme1390I	CCNGG	8 cut(s)	20, 21, 662, 2266, 3693, 3946, 4088, 4300
Bme18I	GGWCC	2 cut(s)	2050, 4623
BmeRI	GACNNNNNGTC	1 cut(s)	1118
BmeT110I	CYCGRG	3 cut(s)	19, 673, 3137
BmgBI	CACGTC	1 cut(s)	3868
BmgT120I	GGNCC	6 cut(s)	1354, 2050, 2663, 4001, 4148, 4623
BmiI	GGNNCC	4 cut(s)	2052, 2342, 4063, 4624
BmrFI	CCNGG	8 cut(s)	20, 21, 662, 2266, 3693, 3946, 4088, 4300
BmtI	GCTAGC	2 cut(s)	2241, 2643
BoxI	GACNNNNGTC	1 cut(s)	2439
BpiI	GAAGAC	2 cut(s)	770, 1006
BplI	GAGNNNNNCTC	2 cut(s)	3654, 3686
BpmI	CTGGAG	3 cut(s)	3101, 3237, 4109
Bpu10I	CCTNAGC	5 cut(s)	2456, 3019, 3554, 3677, 3816
Bpu14I	TTCGAA	1 cut(s)	1948
BpuEI	CTTGAG	9 cut(s)	612, 1144, 1321, 1862, 2393, 2906, 3656, 3815, 4327
BpuMI	CCSGG	3 cut(s)	20, 21, 2266
Bsa29I	ATCGAT	2 cut(s)	1913, 3492
BsaAI	YACGTR	1 cut(s)	487
BsaBI	GATNNNNATC	1 cut(s)	843
BsaHI	GRCGYC	2 cut(s)	274, 1058
BsaI	GGTCTC	1 cut(s)	1653
BsaJI	CCNNGG	8 cut(s)	19, 1784, 2610, 3045, 3101, 3691, 4086, 4264
BsaXI	ACNNNNNCTCC	6 cut(s)	2769, 2799, 3863, 3893, 4519, 4549
Bsc4I	CCNNNNNNNGG	6 cut(s)	357, 1362, 2611, 2748, 3835, 4270
Bse118I	RCCGGY	1 cut(s)	4407
Bse1I	ACTGG	7 cut(s)	1542, 1900, 2100, 2895, 3084, 4275, 4513
Bse21I	CCTNAGG	1 cut(s)	3769
Bse3DI	GCAATG	5 cut(s)	1526, 1639, 3053, 3594, 3903
Bse8I	GATNNNNATC	1 cut(s)	843
BseBI	CCWGG	5 cut(s)	662, 3693, 3946, 4088, 4300
BseCI	ATCGAT	2 cut(s)	1913, 3492
BseDI	CCNNGG	8 cut(s)	19, 1784, 2610, 3045, 3101, 3691, 4086, 4264
BseGI	GGATG	9 cut(s)	366, 907, 991, 1387, 1905, 2302, 2375, 2825, 3564
BseJI	GATNNNNATC	1 cut(s)	843
BseLI	CCNNNNNNNGG	6 cut(s)	357, 1362, 2611, 2748, 3835, 4270
BseMI	GCAATG	5 cut(s)	1526, 1639, 3053, 3594, 3903
BseNI	ACTGG	7 cut(s)	1542, 1900, 2100, 2895, 3084, 4275, 4513
BseRI	GAGGAG	3 cut(s)	179, 1403, 2473
BseSI	GKGCMC	2 cut(s)	1736, 2944
BseXI	GCAGC	8 cut(s)	334, 376, 414, 1214, 2439, 3940, 4247, 4499
BseYI	CCCAGC	3 cut(s)	2665, 3892, 4065
BsgI	GTGCAG	1 cut(s)	3990
BshVI	ATCGAT	2 cut(s)	1913, 3492
BsiHKAI	GWGCWC	3 cut(s)	234, 1736, 2944
BsiHKCI	CYCGRG	3 cut(s)	19, 673, 3137
BsiSI	CCGG	3 cut(s)	20, 2266, 4408
BsiWI	CGTACG	1 cut(s)	1627
BslFI	GGGAC	6 cut(s)	2036, 2248, 2764, 3134, 3192, 3816
BslI	CCNNNNNNNGG	6 cut(s)	357, 1362, 2611, 2748, 3835, 4270
BsmAI	GTCTC	4 cut(s)	1297, 1653, 3044, 3134
BsmBI	CGTCTC	1 cut(s)	1297
BsmFI	GGGAC	6 cut(s)	2036, 2248, 2764, 3134, 3192, 3816
BsmI	GAATGC	8 cut(s)	642, 1528, 1531, 1620, 2602, 3700, 3702, 4107
Bso31I	GGTCTC	1 cut(s)	1653
BsoBI	CYCGRG	3 cut(s)	19, 673, 3137
Bsp119I	TTCGAA	1 cut(s)	1948
Bsp1286I	GDGCHC	3 cut(s)	234, 1736, 2944
Bsp19I	CCATGG	1 cut(s)	4264
BspACI	CCGC	3 cut(s)	2274, 4043, 4582
BspDI	ATCGAT	2 cut(s)	1913, 3492
BspHI	TCATGA	1 cut(s)	2907
BspLI	GGNNCC	4 cut(s)	2052, 2342, 4063, 4624
BspMI	ACCTGC	2 cut(s)	1154, 3699
BspOI	GCTAGC	2 cut(s)	2241, 2643
BspPI	GGATC	7 cut(s)	1178, 3092, 3113, 3141, 3196, 4077, 4617
BspQI	GCTCTTC	1 cut(s)	925
BspT104I	TTCGAA	1 cut(s)	1948
BspTI	CTTAAG	1 cut(s)	1394
BspTNI	GGTCTC	1 cut(s)	1653
BsrBI	CCGCTC	1 cut(s)	4582
BsrDI	GCAATG	5 cut(s)	1526, 1639, 3053, 3594, 3903
BsrFI	RCCGGY	1 cut(s)	4407
BsrI	ACTGG	7 cut(s)	1542, 1900, 2100, 2895, 3084, 4275, 4513
BssAI	RCCGGY	1 cut(s)	4407
BssECI	CCNNGG	8 cut(s)	19, 1784, 2610, 3045, 3101, 3691, 4086, 4264
BssNI	GRCGYC	2 cut(s)	274, 1058
BssT1I	CCWWGG	5 cut(s)	1784, 2610, 3045, 3101, 4264
Bst2UI	CCWGG	5 cut(s)	662, 3693, 3946, 4088, 4300
Bst4CI	ACNGT	9 cut(s)	436, 1252, 1507, 1577, 1956, 2697, 2709, 3302, 3416
Bst6I	CTCTTC	6 cut(s)	925, 1380, 1857, 1932, 4580, 4586
BstACI	GRCGYC	2 cut(s)	274, 1058
BstAFI	CTTAAG	1 cut(s)	1394
BstAPI	GCANNNNNTGC	1 cut(s)	1523
BstBAI	YACGTR	1 cut(s)	487
BstBI	TTCGAA	1 cut(s)	1948
BstC8I	GCNNGC	6 cut(s)	1835, 2239, 2641, 3951, 4147, 4258
BstDSI	CCRYGG	1 cut(s)	4264
BstF5I	GGATG	9 cut(s)	366, 907, 991, 1387, 1905, 2302, 2375, 2825, 3564
BstMAI	GTCTC	4 cut(s)	1297, 1653, 3044, 3134
BstMWI	GCNNNNNNNGC	6 cut(s)	563, 932, 1211, 1283, 1523, 3559
BstNI	CCWGG	5 cut(s)	662, 3693, 3946, 4088, 4300
BstNSI	RCATGY	3 cut(s)	939, 1670, 4055
BstPAI	GACNNNNGTC	1 cut(s)	2439
BstSCI	CCNGG	8 cut(s)	18, 19, 660, 2264, 3691, 3944, 4086, 4298
BstSFI	CTRYAG	4 cut(s)	921, 2693, 2981, 2987
BstSLI	GKGCMC	2 cut(s)	1736, 2944
BstV1I	GCAGC	8 cut(s)	334, 376, 414, 1214, 2439, 3940, 4247, 4499
BstV2I	GAAGAC	2 cut(s)	770, 1006
BstX2I	RGATCY	6 cut(s)	2033, 3133, 3664, 3727, 3759, 4082
BstYI	RGATCY	6 cut(s)	2033, 3133, 3664, 3727, 3759, 4082
Bsu15I	ATCGAT	2 cut(s)	1913, 3492
Bsu36I	CCTNAGG	1 cut(s)	3769
BsuI	GTATCC	1 cut(s)	1340
BsuTUI	ATCGAT	2 cut(s)	1913, 3492
BtgI	CCRYGG	1 cut(s)	4264
BtrI	CACGTC	1 cut(s)	3868
BtsCI	GGATG	9 cut(s)	366, 907, 991, 1387, 1905, 2302, 2375, 2825, 3564
BtsI	GCAGTG	1 cut(s)	4477
BtsIMutI	CAGTG	7 cut(s)	454, 1512, 1535, 1952, 2705, 4244, 4477
BveI	ACCTGC	2 cut(s)	1154, 3699
Cac8I	GCNNGC	6 cut(s)	1835, 2239, 2641, 3951, 4147, 4258
CciI	TCATGA	1 cut(s)	2907
Cfr10I	RCCGGY	1 cut(s)	4407
Cfr13I	GGNCC	6 cut(s)	1354, 2050, 2663, 4001, 4148, 4623
Cfr9I	CCCGGG	1 cut(s)	19
ClaI	ATCGAT	2 cut(s)	1913, 3492
CseI	GACGC	1 cut(s)	282
Csp6I	GTAC	8 cut(s)	499, 658, 833, 1628, 1781, 2117, 3072, 4547
CspCI	CAANNNNNGTGG	4 cut(s)	1204, 1239, 2301, 2336
CviQI	GTAC	8 cut(s)	499, 658, 833, 1628, 1781, 2117, 3072, 4547
DrdI	GACNNNNNNGTC	2 cut(s)	21, 1667
DriI	GACNNNNNGTC	1 cut(s)	1118
DseDI	GACNNNNNNGTC	2 cut(s)	21, 1667
EaeI	YGGCCR	5 cut(s)	1706, 2856, 3713, 3947, 4296
Eam1104I	CTCTTC	6 cut(s)	925, 1380, 1857, 1932, 4580, 4586
Eam1105I	GACNNNNNGTC	1 cut(s)	1118
EarI	CTCTTC	6 cut(s)	925, 1380, 1857, 1932, 4580, 4586
Ecl136II	GAGCTC	1 cut(s)	232
Eco130I	CCWWGG	5 cut(s)	1784, 2610, 3045, 3101, 4264
Eco24I	GRGCYC	1 cut(s)	234
Eco31I	GGTCTC	1 cut(s)	1653
Eco32I	GATATC	1 cut(s)	2892
Eco47I	GGWCC	2 cut(s)	2050, 4623
Eco53kI	GAGCTC	1 cut(s)	232
Eco57I	CTGAAG	6 cut(s)	1797, 2102, 2273, 2592, 3023, 4560
Eco81I	CCTNAGG	1 cut(s)	3769
Eco88I	CYCGRG	3 cut(s)	19, 673, 3137
EcoICRI	GAGCTC	1 cut(s)	232
EcoO109I	RGGNCCY	1 cut(s)	2050
EcoRI	GAATTC	2 cut(s)	867, 4132
EcoRII	CCWGG	5 cut(s)	660, 3691, 3944, 4086, 4298
EcoRV	GATATC	1 cut(s)	2892
EcoT14I	CCWWGG	5 cut(s)	1784, 2610, 3045, 3101, 4264
EcoT22I	ATGCAT	6 cut(s)	1095, 1288, 1528, 1620, 3702, 4119
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	5 cut(s)	1784, 2610, 3045, 3101, 4264
Esp3I	CGTCTC	1 cut(s)	1297
FalI	AAGNNNNNCTT	4 cut(s)	1377, 1409, 4533, 4565
FaqI	GGGAC	6 cut(s)	2036, 2248, 2764, 3134, 3192, 3816
FbaI	TGATCA	1 cut(s)	444
FblI	GTMKAC	1 cut(s)	3486
Fnu4HI	GCNGC	8 cut(s)	348, 365, 428, 1203, 2453, 3954, 4261, 4513
FokI	GGATG	9 cut(s)	373, 914, 998, 1394, 1892, 2309, 2382, 2832, 3571
FriOI	GRGCYC	1 cut(s)	234
Fsp4HI	GCNGC	8 cut(s)	348, 365, 428, 1203, 2453, 3954, 4261, 4513
GluI	GCNGC	8 cut(s)	348, 365, 428, 1203, 2453, 3954, 4261, 4513
GsaI	CCCAGC	3 cut(s)	2669, 3896, 4069
GsuI	CTGGAG	3 cut(s)	3101, 3237, 4109
HapII	CCGG	3 cut(s)	20, 2266, 4408
HgaI	GACGC	1 cut(s)	282
Hin1I	GRCGYC	2 cut(s)	274, 1058
HincII	GTYRAC	6 cut(s)	1056, 1501, 2500, 3283, 4477, 4527
HindII	GTYRAC	6 cut(s)	1056, 1501, 2500, 3283, 4477, 4527
HindIII	AAGCTT	6 cut(s)	1014, 2143, 2160, 2723, 3187, 3674
HpaI	GTTAAC	2 cut(s)	2500, 3283
HpaII	CCGG	3 cut(s)	20, 2266, 4408
HphI	GGTGA	4 cut(s)	1466, 2089, 2369, 3238
HpyCH4III	ACNGT	9 cut(s)	436, 1252, 1507, 1577, 1956, 2697, 2709, 3302, 3416
HpyCH4IV	ACGT	6 cut(s)	111, 486, 1058, 1497, 3867, 4395
HpyF10VI	GCNNNNNNNGC	6 cut(s)	563, 932, 1211, 1283, 1523, 3559
HpySE526I	ACGT	6 cut(s)	111, 486, 1058, 1497, 3867, 4395
Hsp92I	GRCGYC	2 cut(s)	274, 1058
Ksp22I	TGATCA	1 cut(s)	444
KspAI	GTTAAC	2 cut(s)	2500, 3283
LguI	GCTCTTC	1 cut(s)	925
LmnI	GCTCC	6 cut(s)	44, 1390, 3256, 4074, 4090, 4126
Lsp1109I	GCAGC	8 cut(s)	334, 376, 414, 1214, 2439, 3940, 4247, 4499
MaeII	ACGT	6 cut(s)	111, 486, 1058, 1497, 3867, 4395
MaeIII	GTNAC	8 cut(s)	947, 1246, 1367, 2575, 2616, 2761, 3226, 3643
MbiI	CCGCTC	1 cut(s)	4582
MfeI	CAATTG	2 cut(s)	267, 4056
MflI	RGATCY	6 cut(s)	2033, 3133, 3664, 3727, 3759, 4082
MhlI	GDGCHC	3 cut(s)	234, 1736, 2944
MlsI	TGGCCA	5 cut(s)	1708, 2858, 3715, 3949, 4298
MluNI	TGGCCA	5 cut(s)	1708, 2858, 3715, 3949, 4298
MlyI	GAGTC	8 cut(s)	665, 1893, 2068, 2624, 2722, 3116, 3264, 3603
MmeI	TCCRAC	7 cut(s)	1359, 1866, 1975, 2070, 2412, 2817, 3382
Mox20I	TGGCCA	5 cut(s)	1708, 2858, 3715, 3949, 4298
Mph1103I	ATGCAT	6 cut(s)	1095, 1288, 1528, 1620, 3702, 4119
MroXI	GAANNNNTTC	3 cut(s)	2600, 3156, 3700
MscI	TGGCCA	5 cut(s)	1708, 2858, 3715, 3949, 4298
MslI	CAYNNNNRTG	6 cut(s)	945, 2368, 2553, 2820, 3218, 4112
Msp20I	TGGCCA	5 cut(s)	1708, 2858, 3715, 3949, 4298
MspA1I	CMGCKG	2 cut(s)	3553, 3953
MspCI	CTTAAG	1 cut(s)	1394
MspI	CCGG	3 cut(s)	20, 2266, 4408
MspR9I	CCNGG	8 cut(s)	20, 21, 662, 2266, 3693, 3946, 4088, 4300
MunI	CAATTG	2 cut(s)	267, 4056
Mva1269I	GAATGC	8 cut(s)	642, 1528, 1531, 1620, 2602, 3700, 3702, 4107
MvaI	CCWGG	5 cut(s)	662, 3693, 3946, 4088, 4300
MwoI	GCNNNNNNNGC	6 cut(s)	563, 932, 1211, 1283, 1523, 3559
NciI	CCSGG	3 cut(s)	20, 21, 2266
NcoI	CCATGG	1 cut(s)	4264
NheI	GCTAGC	2 cut(s)	2237, 2639
NlaIV	GGNNCC	4 cut(s)	2052, 2342, 4063, 4624
NmuCI	GTSAC	3 cut(s)	947, 2616, 3226
NsiI	ATGCAT	6 cut(s)	1095, 1288, 1528, 1620, 3702, 4119
NspI	RCATGY	3 cut(s)	939, 1670, 4055
NspV	TTCGAA	1 cut(s)	1948
PaeR7I	CTCGAG	2 cut(s)	673, 3137
PagI	TCATGA	1 cut(s)	2907
PaqCI	CACCTGC	2 cut(s)	1154, 3699
PciI	ACATGT	1 cut(s)	1666
PciSI	GCTCTTC	1 cut(s)	925
PctI	GAATGC	8 cut(s)	642, 1528, 1531, 1620, 2602, 3700, 3702, 4107
PdmI	GAANNNNTTC	3 cut(s)	2600, 3156, 3700
Pfl23II	CGTACG	1 cut(s)	1627
PflMI	CCANNNNNTGG	1 cut(s)	4270
PkrI	GCNGC	8 cut(s)	349, 366, 429, 1204, 2454, 3955, 4262, 4514
PleI	GAGTC	8 cut(s)	665, 1893, 2068, 2623, 2721, 3116, 3264, 3603
PpsI	GAGTC	8 cut(s)	665, 1893, 2068, 2623, 2721, 3116, 3264, 3603
Ppu21I	YACGTR	1 cut(s)	487
PpuMI	RGGWCCY	1 cut(s)	2050
PscI	ACATGT	1 cut(s)	1666
PshAI	GACNNNNGTC	1 cut(s)	2439
PshBI	ATTAAT	1 cut(s)	1599
PsiI	TTATAA	2 cut(s)	2967, 4097
Psp124BI	GAGCTC	1 cut(s)	234
Psp1406I	AACGTT	2 cut(s)	111, 1497
Psp5II	RGGWCCY	1 cut(s)	2050
Psp6I	CCWGG	5 cut(s)	660, 3691, 3944, 4086, 4298
PspFI	CCCAGC	3 cut(s)	2665, 3892, 4065
PspGI	CCWGG	5 cut(s)	660, 3691, 3944, 4086, 4298
PspLI	CGTACG	1 cut(s)	1627
PspN4I	GGNNCC	4 cut(s)	2052, 2342, 4063, 4624
PspPI	GGNCC	6 cut(s)	1354, 2050, 2663, 4001, 4148, 4623
PspPPI	RGGWCCY	1 cut(s)	2050
PspXI	VCTCGAGB	1 cut(s)	673
PsuI	RGATCY	6 cut(s)	2033, 3133, 3664, 3727, 3759, 4082
PvuII	CAGCTG	2 cut(s)	3553, 3953
RsaI	GTAC	8 cut(s)	500, 659, 834, 1629, 1782, 2118, 3073, 4548
RsaNI	GTAC	8 cut(s)	499, 658, 833, 1628, 1781, 2117, 3072, 4547
RseI	CAYNNNNRTG	6 cut(s)	945, 2368, 2553, 2820, 3218, 4112
SacI	GAGCTC	1 cut(s)	234
SapI	GCTCTTC	1 cut(s)	925
SatI	GCNGC	8 cut(s)	348, 365, 428, 1203, 2453, 3954, 4261, 4513
Sau96I	GGNCC	6 cut(s)	1354, 2050, 2663, 4001, 4148, 4623
ScaI	AGTACT	1 cut(s)	834
SchI	GAGTC	8 cut(s)	665, 1893, 2068, 2624, 2722, 3116, 3264, 3603
ScrFI	CCNGG	8 cut(s)	20, 21, 662, 2266, 3693, 3946, 4088, 4300
SduI	GDGCHC	3 cut(s)	234, 1736, 2944
SfcI	CTRYAG	4 cut(s)	921, 2693, 2981, 2987
Sfr274I	CTCGAG	2 cut(s)	673, 3137
SfuI	TTCGAA	1 cut(s)	1948
SinI	GGWCC	2 cut(s)	2050, 4623
SlaI	CTCGAG	2 cut(s)	673, 3137
SmaI	CCCGGG	1 cut(s)	21
SmiMI	CAYNNNNRTG	6 cut(s)	945, 2368, 2553, 2820, 3218, 4112
SpeI	ACTAGT	1 cut(s)	4037
SsiI	CCGC	3 cut(s)	2274, 4043, 4582
SspI	AATATT	5 cut(s)	1444, 3068, 3456, 4017, 4277
SstI	GAGCTC	1 cut(s)	234
StyD4I	CCNGG	8 cut(s)	18, 19, 660, 2264, 3691, 3944, 4086, 4298
StyI	CCWWGG	5 cut(s)	1784, 2610, 3045, 3101, 4264
TaaI	ACNGT	9 cut(s)	436, 1252, 1507, 1577, 1956, 2697, 2709, 3302, 3416
TaiI	ACGT	6 cut(s)	114, 489, 1061, 1500, 3870, 4398
TatI	WGTACW	4 cut(s)	832, 2116, 3071, 4546
TscAI	CASTG	7 cut(s)	454, 1512, 1542, 1959, 2712, 4251, 4477
TseFI	GTSAC	3 cut(s)	947, 2616, 3226
TseI	GCWGC	8 cut(s)	347, 364, 427, 1202, 2452, 3953, 4260, 4512
Tsp45I	GTSAC	3 cut(s)	947, 2616, 3226
TspGWI	ACGGA	1 cut(s)	4631
TspMI	CCCGGG	1 cut(s)	19
TspRI	CASTG	7 cut(s)	454, 1512, 1542, 1959, 2712, 4251, 4477
Van91I	CCANNNNNTGG	1 cut(s)	4270
Vha464I	CTTAAG	1 cut(s)	1394
VneI	GTGCAC	2 cut(s)	1732, 2940
VpaK11BI	GGWCC	2 cut(s)	2050, 4623
VspI	ATTAAT	1 cut(s)	1599
XapI	RAATTY	8 cut(s)	867, 2016, 2068, 3734, 4132, 4170, 4204, 4234
XbaI	TCTAGA	5 cut(s)	1061, 1655, 1960, 3349, 4174
XceI	RCATGY	3 cut(s)	939, 1670, 4055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2351
XhoI	CTCGAG	2 cut(s)	673, 3137
XmaI	CCCGGG	1 cut(s)	19
XmaJI	CCTAGG	1 cut(s)	3101
XmiI	GTMKAC	1 cut(s)	3486
XmnI	GAANNNNTTC	3 cut(s)	2600, 3156, 3700
ZraI	GACGTC	1 cut(s)	1059
ZrmI	AGTACT	1 cut(s)	834
Zsp2I	ATGCAT	6 cut(s)	1095, 1288, 1528, 1620, 3702, 4119

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.