MD03G1127700.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003793
Physical Location & Seq
Reverse (-)
12419551 .. 12425127
5577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1127700.v1.1.491

Sequence Viewer

Length: 2592 bp
ATGGCTATTGCTGGTCCACACAATGTTTCTGTGCTTGAACCTTCCTTCCTTAGAGACTCTCAGTCTCATTCATCTAGGCGAATACGTGACGAAGGGAGCATGAGTACCCGCGCATCCTCCCTGCTACGAAGGTGGCGAGAGCTTGAGGATGAGCATGTCTCGAGTCGTTCCCAAGAGAGAGATAGTGAGAGGTTGATTCTACAAAGGAGTGATAGGTTGATAGATGAGATCTCGAGAGCAGACACTACTGAAGACCACGGTAGTGACAACCCAGGTGATTTGGAGGATGTGAGAGTGGGTGAGAGTGAGTGCCGATTATGGTCCCGAGGTCAAATTGGGTCGCCAAATGAACATGAGGAGTGCAGTAATTTTAGCAGTGAGCATTCTGATTTTGGGGAGGTTGAAAGGGGGAGGGTGAGGCAAGTTTTCAGGGAATGGATGGCTTGTGGTGGGGCTGAATATATGTCAAATGGTTCTCGTTTGAATAACAATTCAAGGGCAGACTGGCTTGGTGAAACTGAACAGGAGAGGGTGAGAATTGTAAGGGAGTGGGTGCGACTTAACAGCCAGCAGAGAGGTGCTTCTGGTGACAATAGAGGAGAACAACCTGCTGAAATTGGTAATCAAATTGAAAGAGTTCGTGATGGATTGATTGTTAACCAAACTGAAGGCCGGTCTGGGCATACACGAAGGGGAATTCGTAAATTATGTGGTAGACAGGCTATGCTGGATATGGTAAAGAAGGCTGAGAGGGAAAGACAAAGGGAACTTCAAGTCTTGTTGGAGCACCAGGCTGTATCACAGTTTGCTCACCGCAACCGCATTCAGTCATTGCTTAGAGGAAGATTCTTACGAAATGCTAGAGTGATTGAGGATGAGAGACCTACTTCCATGGCTGAAAATGAATTAGGTTTATTGAGACAAAGGCACACAGTCTCTGGTCTAAGGGACGGTTTTTGCTCCAGATTGGATAGTTCTGTTTCTGGTCCAGTAAGCAGCAGCCACTCTGAGACATCTTCTAGTGTTTCTATTGGTAGTAGAAATGGCGATACTCAAGTAGACAACACACATGAGGTCCTAGCTGGGTCTTCTGAACAATCTGCCACTAATGATGAGGCAAGTGAGGACCGAGAACATCGCTGTGGTATATCAGATCGCACGGATTTAGGTGGCAACGCCGTTCAAGACATAGATTCAGAAGAACCTGATGCTCCTGTAGAGCGCTGGCAAGAAGAAGTTCCAGATAATGTTGTAAGAGACTGGCAATGCTCAACTATCACTGAATTTATTGAAAGGAGGGATGGTACTGGTCCTAACATGATTGGGGACTTACATACAACCACTGCCGTTGAGCAGCCCCCAGAAACTTTGCAAGATGATGCTGGACACAGTAACATGCGAGAAGTCAGTGATATGTCTAATGAGCAGTCTGAGCCGAGTGGGGAGGCAAGTGTAATAGGTGAACGATCTGATCATACATATAATTTACGAGGAACTGTGCTTGGGGATGTAAATTTTCAAGAATTTACTTCTCAGGTACAACAATGGCAGGATCAAGTTTCAGAAAATGCCGAAGGGGACTTGGAACAACCTGTTGTTGAGTATAATGACTTGAGAGAAAACGTTGGGGAAGATATAGCTGAGGAACAGCAGGAAACAACTGCTTATGAATGGCCCCAGGAGTTGTTGGAGAGCGAAGATAGAGAAAACAGTCGTATTGAAGAAGTTTCTGAAGTTTGGCATGAGGAAAGTGGTTTTCAGGAGGCTGTTCAAAGTTGGTTAGAAGAGCCATCGGACCAGGATGTTTATCCGGTCAGGCAGATTGATACATTTTATTTTCCGGATGATGATAATGCTCCTGGTACTGAGCTAAGGGAACTCCTAAGCAGGAGAAGGGTCTCTAATCTTCTTAGTAGTGGTTTCCGTCAGAATCTAGATCGACTGTTACAATCATATGTGGAAAGGCAAAGTCATGCTGCCATTGACTGGGAGCTGGATGATACATCACCTTCTCCTGAATCTGCAGAACAGGATCTAGAGCAGACTGGTGCATATCGAAATGGGGGTCAAGTGCATGCTGTTGAGAGTCGTAGTCCTAGTACTGCTCTACGATCACAGCAAATACCTTCCTCTTCACTTTGGGACCAGGAGTCACACCCTGATAATTGGCCACAGCATGACATGCATCAACGTTATGGAATTGACTGGGATATAATTAACGATATGAGGATTGACATGGCCAGGCTACAACAGAGGATGAATAACTTGCAACGAATGATGGAGGCCTGCATGGATATGCAACTTGAGTTGCAGCGCTCGATAAGACAGGAAGTTTCTGATGCCCTGAATAGATCAGCTGGTTCACAAGGGGCGTGTCAAGACGGTCTGCTAGAAGATGGGTCTAAATGGGATCATGTAAGGAAAGGAATTTGCTGTATATGTTGTGAAAGCAGCATCGATTCTTTATTGTACAGATGTGGGCACATGTGCACATGTTCACAATGTGCTACCAAATTGGTTGAGAGTAGCGGAAAATGTCCGATGTGTAGAGCACCCGCAATTGAGGTGATTCGTGCTTATTCCGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

864

Amino Acids

97.91

Weight (kDa)

4.73

Isoelectric Point (pI)

66.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 809 - 853 1.2e-10 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 616
AccB7I CCANNNNNTGG 1 cut(s) 1986
AccI GTMKAC 2 cut(s) 715, 1059
AccII CGCG 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 1840
AciI CCGC 5 cut(s) 109, 814, 820, 2529, 2556
AclI AACGTT 2 cut(s) 1623, 2191
AclWI GGATC 3 cut(s) 1560, 2040, 2418
AcoI YGGCCR 2 cut(s) 2168, 2238
AcsI RAATTY 5 cut(s) 696, 1283, 1513, 1523, 2427
AcuI CTGAAG 3 cut(s) 270, 687, 1752
AdeI CACNNNGTG 1 cut(s) 2504
AfaI GTAC 6 cut(s) 106, 1306, 1539, 1864, 2101, 2471
AfeI AGCGCT 2 cut(s) 1223, 2315
AfiI CCNNNNNNNGG 1 cut(s) 1986
AflIII ACRYGT 2 cut(s) 2484, 2492
AhdI GACNNNNNGTC 2 cut(s) 61, 2149
AjnI CCWGG 7 cut(s) 271, 789, 1677, 1797, 1858, 2145, 2240
AleI CACNNNNGTG 1 cut(s) 261
AloI GAACNNNNNNTCC 2 cut(s) 30, 62
AluBI AGCT 6 cut(s) 142, 1082, 1640, 1870, 1993, 2357
AluI AGCT 6 cut(s) 142, 1082, 1640, 1870, 1993, 2357
Alw21I GWGCWC 3 cut(s) 789, 2492, 2554
Alw26I GTCTC 9 cut(s) 48, 69, 163, 874, 913, 940, 1004, 1251, 1903
Alw44I GTGCAC 1 cut(s) 2488
AlwI GGATC 3 cut(s) 1560, 2040, 2418
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 3 cut(s) 160, 232, 324
Aor13HI TCCGGA 1 cut(s) 1840
Aor51HI AGCGCT 2 cut(s) 1223, 2315
AoxI GGCC 5 cut(s) 670, 1673, 2168, 2238, 2283
ApaLI GTGCAC 1 cut(s) 2488
ApeKI GCWGC 6 cut(s) 996, 999, 1354, 1976, 2311, 2451
ApoI RAATTY 5 cut(s) 696, 1283, 1513, 1523, 2427
ArsI GACNNNNNNTTYG 2 cut(s) 2050, 2082
Asp700I GAANNNNTTC 2 cut(s) 636, 1236
AspLEI GCGC 3 cut(s) 113, 1224, 2316
AspS9I GGNCC 9 cut(s) 14, 321, 986, 1075, 1126, 1310, 1674, 1795, 2143
AvaI CYCGRG 3 cut(s) 160, 232, 324
AvaII GGWCC 8 cut(s) 14, 321, 986, 1075, 1126, 1310, 1795, 2143
BaeGI GKGCMC 2 cut(s) 2484, 2492
BaeI ACNNNNGTAYC 2 cut(s) 1041, 1074
BalI TGGCCA 2 cut(s) 2170, 2240
BbsI GAAGAC 2 cut(s) 258, 1080
Bbv12I GWGCWC 3 cut(s) 789, 2492, 2554
BbvCI CCTCAGC 1 cut(s) 1641
BbvI GCAGC 6 cut(s) 1008, 1011, 1366, 1963, 2323, 2463
BccI CCATC 6 cut(s) 433, 638, 1295, 1798, 2272, 2390
BceAI ACGGC 2 cut(s) 1163, 1331
BciT130I CCWGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
BclI TGATCA 1 cut(s) 1471
BcoDI GTCTC 9 cut(s) 48, 69, 163, 874, 913, 940, 1004, 1251, 1903
BfaI CTAG 8 cut(s) 75, 861, 1020, 1079, 1934, 2036, 2097, 2390
BfmI CTRYAG 2 cut(s) 1215, 2022
BfoI RGCGCY 2 cut(s) 1225, 2317
BfuAI ACCTGC 1 cut(s) 616
BglII AGATCT 1 cut(s) 228
BisI GCNGC 6 cut(s) 997, 1000, 1355, 1977, 2312, 2452
BlsI GCNGC 6 cut(s) 998, 1001, 1356, 1978, 2313, 2453
BmcAI AGTACT 1 cut(s) 2101
Bme1390I CCNGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
Bme18I GGWCC 8 cut(s) 14, 321, 986, 1075, 1126, 1310, 1795, 2143
BmeRI GACNNNNNGTC 2 cut(s) 61, 2149
BmeT110I CYCGRG 3 cut(s) 160, 232, 324
BmgT120I GGNCC 9 cut(s) 14, 321, 986, 1075, 1126, 1310, 1674, 1795, 2143
BmiI GGNNCC 3 cut(s) 323, 1676, 2144
BmrFI CCNGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
BmrI ACTGGG 2 cut(s) 1996, 2215
BmsI GCATC 6 cut(s) 122, 1198, 1369, 2194, 2329, 2463
BmuI ACTGGG 2 cut(s) 1996, 2215
BpiI GAAGAC 2 cut(s) 258, 1080
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BpmI CTGGAG 1 cut(s) 946
Bpu10I CCTNAGC 3 cut(s) 1641, 1871, 1883
BpuEI CTTGAG 4 cut(s) 164, 1038, 1633, 2324
Bsa29I ATCGAT 1 cut(s) 2457
BsaAI YACGTR 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 1806
BsaI GGTCTC 2 cut(s) 874, 1903
BsaJI CCNNGG 5 cut(s) 256, 271, 325, 891, 1677
BsaWI WCCGGW 2 cut(s) 1810, 1840
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 1288, 1318
Bsc4I CCNNNNNNNGG 1 cut(s) 1986
Bse118I RCCGGY 1 cut(s) 672
Bse1I ACTGG 7 cut(s) 509, 989, 1265, 1312, 1991, 2050, 2210
Bse3DI GCAATG 2 cut(s) 830, 1271
Bse8I GATNNNNATC 1 cut(s) 1806
BseAI TCCGGA 1 cut(s) 1840
BseBI CCWGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
BseCI ATCGAT 1 cut(s) 2457
BseDI CCNNGG 5 cut(s) 256, 271, 325, 891, 1677
BseJI GATNNNNATC 1 cut(s) 1806
BseLI CCNNNNNNNGG 1 cut(s) 1986
BseMI GCAATG 2 cut(s) 830, 1271
BseMII CTCAG 7 cut(s) 74, 738, 999, 1422, 1547, 1632, 1857
BseNI ACTGG 7 cut(s) 509, 989, 1265, 1312, 1991, 2050, 2210
BseRI GAGGAG 2 cut(s) 371, 612
BseSI GKGCMC 2 cut(s) 2484, 2492
BseXI GCAGC 6 cut(s) 1008, 1011, 1366, 1963, 2323, 2463
BseYI CCCAGC 1 cut(s) 1082
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 5 cut(s) 672, 1675, 2170, 2240, 2285
BshVI ATCGAT 1 cut(s) 2457
BsiHKAI GWGCWC 3 cut(s) 789, 2492, 2554
BsiHKCI CYCGRG 3 cut(s) 160, 232, 324
BsiSI CCGG 3 cut(s) 673, 1811, 1841
BslFI GGGAC 5 cut(s) 307, 962, 1340, 1592, 2156
BslI CCNNNNNNNGG 1 cut(s) 1986
BsmAI GTCTC 9 cut(s) 48, 69, 163, 874, 913, 940, 1004, 1251, 1903
BsmFI GGGAC 5 cut(s) 307, 962, 1340, 1592, 2156
BsmI GAATGC 2 cut(s) 382, 822
BsnI GGCC 5 cut(s) 672, 1675, 2170, 2240, 2285
Bso31I GGTCTC 2 cut(s) 874, 1903
BsoBI CYCGRG 3 cut(s) 160, 232, 324
Bsp1286I GDGCHC 4 cut(s) 789, 2484, 2492, 2554
Bsp13I TCCGGA 1 cut(s) 1840
Bsp1407I TGTACA 1 cut(s) 2469
Bsp19I CCATGG 1 cut(s) 891
BspACI CCGC 5 cut(s) 109, 814, 820, 2529, 2556
BspANI GGCC 5 cut(s) 672, 1675, 2170, 2240, 2285
BspCNI CTCAG 7 cut(s) 73, 739, 1000, 1423, 1546, 1633, 1858
BspDI ATCGAT 1 cut(s) 2457
BspEI TCCGGA 1 cut(s) 1840
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 323, 1676, 2144
BspMAI CTGCAG 1 cut(s) 2026
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 3 cut(s) 1560, 2040, 2418
BspQI GCTCTTC 1 cut(s) 1779
BspTNI GGTCTC 2 cut(s) 874, 1903
BsrDI GCAATG 2 cut(s) 830, 1271
BsrFI RCCGGY 1 cut(s) 672
BsrGI TGTACA 1 cut(s) 2469
BsrI ACTGG 7 cut(s) 509, 989, 1265, 1312, 1991, 2050, 2210
BssAI RCCGGY 1 cut(s) 672
BssECI CCNNGG 5 cut(s) 256, 271, 325, 891, 1677
BssT1I CCWWGG 1 cut(s) 891
Bst2UI CCWGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
Bst4CI ACNGT 9 cut(s) 260, 804, 934, 953, 1391, 1498, 1712, 1944, 2384
Bst6I CTCTTC 2 cut(s) 1779, 2137
BstAPI GCANNNNNTGC 1 cut(s) 2182
BstAUI TGTACA 1 cut(s) 2469
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 4 cut(s) 569, 1226, 2076, 2287
BstDSI CCRYGG 2 cut(s) 256, 891
BstFNI CGCG 1 cut(s) 111
BstH2I RGCGCY 2 cut(s) 1225, 2317
BstHHI GCGC 3 cut(s) 113, 1224, 2316
BstMAI GTCTC 9 cut(s) 48, 69, 163, 874, 913, 940, 1004, 1251, 1903
BstMWI GCNNNNNNNGC 2 cut(s) 1432, 2182
BstNI CCWGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
BstNSI RCATGY 6 cut(s) 158, 1399, 2078, 2185, 2488, 2496
BstSCI CCNGG 7 cut(s) 271, 789, 1677, 1797, 1858, 2145, 2240
BstSFI CTRYAG 2 cut(s) 1215, 2022
BstSLI GKGCMC 2 cut(s) 2484, 2492
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 6 cut(s) 1008, 1011, 1366, 1963, 2323, 2463
BstV2I GAAGAC 2 cut(s) 258, 1080
BstX2I RGATCY 2 cut(s) 228, 2032
BstYI RGATCY 2 cut(s) 228, 2032
Bsu15I ATCGAT 1 cut(s) 2457
BsuRI GGCC 5 cut(s) 672, 1675, 2170, 2240, 2285
BsuTUI ATCGAT 1 cut(s) 2457
BtgI CCRYGG 2 cut(s) 256, 891
BtgZI GCGATG 1 cut(s) 1121
BtsI GCAGTG 2 cut(s) 382, 1341
BtsIMutI CAGTG 4 cut(s) 382, 1278, 1341, 1414
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 4 cut(s) 569, 1226, 2076, 2287
CaiI CAGNNNCTG 1 cut(s) 938
CfoI GCGC 3 cut(s) 113, 1224, 2316
Cfr10I RCCGGY 1 cut(s) 672
Cfr13I GGNCC 9 cut(s) 14, 321, 986, 1075, 1126, 1310, 1674, 1795, 2143
ClaI ATCGAT 1 cut(s) 2457
Csp6I GTAC 6 cut(s) 105, 1305, 1538, 1863, 2100, 2470
CspCI CAANNNNNGTGG 2 cut(s) 1330, 1365
CviQI GTAC 6 cut(s) 105, 1305, 1538, 1863, 2100, 2470
DraIII CACNNNGTG 1 cut(s) 2504
DriI GACNNNNNGTC 2 cut(s) 61, 2149
EaeI YGGCCR 2 cut(s) 2168, 2238
Eam1104I CTCTTC 2 cut(s) 1779, 2137
Eam1105I GACNNNNNGTC 2 cut(s) 61, 2149
EarI CTCTTC 2 cut(s) 1779, 2137
Eco130I CCWWGG 1 cut(s) 891
Eco147I AGGCCT 1 cut(s) 2285
Eco31I GGTCTC 2 cut(s) 874, 1903
Eco47I GGWCC 8 cut(s) 14, 321, 986, 1075, 1126, 1310, 1795, 2143
Eco47III AGCGCT 2 cut(s) 1223, 2315
Eco57I CTGAAG 3 cut(s) 270, 687, 1752
Eco88I CYCGRG 3 cut(s) 160, 232, 324
EcoO109I RGGNCCY 1 cut(s) 1075
EcoRI GAATTC 1 cut(s) 696
EcoRII CCWGG 7 cut(s) 271, 789, 1677, 1797, 1858, 2145, 2240
EcoT14I CCWWGG 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 2187
ErhI CCWWGG 1 cut(s) 891
FaqI GGGAC 5 cut(s) 307, 962, 1340, 1592, 2156
FauI CCCGC 2 cut(s) 116, 2563
FauNDI CATATG 1 cut(s) 1954
FbaI TGATCA 1 cut(s) 1471
FblI GTMKAC 2 cut(s) 715, 1059
Fnu4HI GCNGC 6 cut(s) 997, 1000, 1355, 1977, 2312, 2452
Fsp4HI GCNGC 6 cut(s) 997, 1000, 1355, 1977, 2312, 2452
FspBI CTAG 8 cut(s) 75, 861, 1020, 1079, 1934, 2036, 2097, 2390
GlaI GCGC 3 cut(s) 112, 1223, 2315
GluI GCNGC 6 cut(s) 997, 1000, 1355, 1977, 2312, 2452
GsaI CCCAGC 1 cut(s) 1086
GsuI CTGGAG 1 cut(s) 946
HaeII RGCGCY 2 cut(s) 1225, 2317
HaeIII GGCC 5 cut(s) 672, 1675, 2170, 2240, 2285
HapII CCGG 3 cut(s) 673, 1811, 1841
HhaI GCGC 3 cut(s) 113, 1224, 2316
Hin6I GCGC 3 cut(s) 111, 1222, 2314
HinP1I GCGC 3 cut(s) 111, 1222, 2314
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 3 cut(s) 673, 1811, 1841
Hpy166II GTNNAC 8 cut(s) 17, 658, 716, 1060, 1463, 2363, 2490, 2498
Hpy8I GTNNAC 8 cut(s) 17, 658, 716, 1060, 1463, 2363, 2490, 2498
HpyCH4III ACNGT 9 cut(s) 260, 804, 934, 953, 1391, 1498, 1712, 1944, 2384
HpyCH4IV ACGT 3 cut(s) 85, 1623, 2191
HpyF10VI GCNNNNNNNGC 2 cut(s) 1432, 2182
HpySE526I ACGT 3 cut(s) 85, 1623, 2191
HspAI GCGC 3 cut(s) 111, 1222, 2314
Kpn2I TCCGGA 1 cut(s) 1840
Ksp22I TGATCA 1 cut(s) 1471
KspAI GTTAAC 1 cut(s) 658
LguI GCTCTTC 1 cut(s) 1779
LmnI GCTCC 6 cut(s) 96, 784, 965, 1216, 1861, 1990
Lsp1109I GCAGC 6 cut(s) 1008, 1011, 1366, 1963, 2323, 2463
LweI GCATC 6 cut(s) 122, 1198, 1369, 2194, 2329, 2463
MaeI CTAG 8 cut(s) 75, 861, 1020, 1079, 1934, 2036, 2097, 2390
MaeII ACGT 3 cut(s) 85, 1623, 2191
MaeIII GTNAC 6 cut(s) 86, 263, 587, 1391, 1944, 2151
MfeI CAATTG 1 cut(s) 2559
MflI RGATCY 2 cut(s) 228, 2032
MhlI GDGCHC 4 cut(s) 789, 2484, 2492, 2554
MlsI TGGCCA 2 cut(s) 2170, 2240
MluNI TGGCCA 2 cut(s) 2170, 2240
MlyI GAGTC 4 cut(s) 50, 172, 2095, 2159
MmeI TCCRAC 2 cut(s) 762, 1668
Mox20I TGGCCA 2 cut(s) 2170, 2240
Mph1103I ATGCAT 1 cut(s) 2187
MroI TCCGGA 1 cut(s) 1840
MroXI GAANNNNTTC 2 cut(s) 636, 1236
MscI TGGCCA 2 cut(s) 2170, 2240
MseI TTAA 3 cut(s) 561, 657, 2217
MslI CAYNNNNRTG 3 cut(s) 261, 1140, 2294
Msp20I TGGCCA 2 cut(s) 2170, 2240
MspA1I CMGCKG 1 cut(s) 2357
MspI CCGG 3 cut(s) 673, 1811, 1841
MspR9I CCNGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
MunI CAATTG 1 cut(s) 2559
Mva1269I GAATGC 2 cut(s) 382, 822
MvaI CCWGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 1432, 2182
NcoI CCATGG 1 cut(s) 891
NdeI CATATG 1 cut(s) 1954
NlaIV GGNNCC 3 cut(s) 323, 1676, 2144
NmeAIII GCCGAG 1 cut(s) 1461
NmuCI GTSAC 4 cut(s) 86, 263, 587, 2151
NsiI ATGCAT 1 cut(s) 2187
NspI RCATGY 6 cut(s) 158, 1399, 2078, 2185, 2488, 2496
OliI CACNNNNGTG 1 cut(s) 261
PaeI GCATGC 1 cut(s) 2078
PaeR7I CTCGAG 2 cut(s) 160, 232
PceI AGGCCT 1 cut(s) 2285
PciI ACATGT 2 cut(s) 2484, 2492
PciSI GCTCTTC 1 cut(s) 1779
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PctI GAATGC 2 cut(s) 382, 822
PdmI GAANNNNTTC 2 cut(s) 636, 1236
PfeI GAWTC 7 cut(s) 196, 846, 1193, 1930, 2018, 2459, 2569
PflMI CCANNNNNTGG 1 cut(s) 1986
PkrI GCNGC 6 cut(s) 998, 1001, 1356, 1978, 2313, 2453
PleI GAGTC 4 cut(s) 50, 171, 2094, 2158
PpsI GAGTC 4 cut(s) 50, 171, 2094, 2158
Ppu21I YACGTR 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 1075
PscI ACATGT 2 cut(s) 2484, 2492
Psp1406I AACGTT 2 cut(s) 1623, 2191
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 7 cut(s) 271, 789, 1677, 1797, 1858, 2145, 2240
PspFI CCCAGC 1 cut(s) 1082
PspGI CCWGG 7 cut(s) 271, 789, 1677, 1797, 1858, 2145, 2240
PspN4I GGNNCC 3 cut(s) 323, 1676, 2144
PspPI GGNCC 9 cut(s) 14, 321, 986, 1075, 1126, 1310, 1674, 1795, 2143
PspPPI RGGWCCY 1 cut(s) 1075
PstI CTGCAG 1 cut(s) 2026
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 2 cut(s) 228, 2032
PvuII CAGCTG 1 cut(s) 2357
RsaI GTAC 6 cut(s) 106, 1306, 1539, 1864, 2101, 2471
RsaNI GTAC 6 cut(s) 105, 1305, 1538, 1863, 2100, 2470
RseI CAYNNNNRTG 3 cut(s) 261, 1140, 2294
SapI GCTCTTC 1 cut(s) 1779
SaqAI TTAA 3 cut(s) 561, 657, 2217
SatI GCNGC 6 cut(s) 997, 1000, 1355, 1977, 2312, 2452
Sau96I GGNCC 9 cut(s) 14, 321, 986, 1075, 1126, 1310, 1674, 1795, 2143
ScaI AGTACT 1 cut(s) 2101
SchI GAGTC 4 cut(s) 50, 172, 2095, 2159
ScrFI CCNGG 7 cut(s) 273, 791, 1679, 1799, 1860, 2147, 2242
SduI GDGCHC 4 cut(s) 789, 2484, 2492, 2554
SfaNI GCATC 6 cut(s) 122, 1198, 1369, 2194, 2329, 2463
SfcI CTRYAG 2 cut(s) 1215, 2022
Sfr274I CTCGAG 2 cut(s) 160, 232
SinI GGWCC 8 cut(s) 14, 321, 986, 1075, 1126, 1310, 1795, 2143
SlaI CTCGAG 2 cut(s) 160, 232
SmiMI CAYNNNNRTG 3 cut(s) 261, 1140, 2294
SmlI CTYRAG 6 cut(s) 143, 160, 232, 1053, 1612, 2303
SmoI CTYRAG 6 cut(s) 143, 160, 232, 1053, 1612, 2303
SphI GCATGC 1 cut(s) 2078
SseBI AGGCCT 1 cut(s) 2285
SsiI CCGC 5 cut(s) 109, 814, 820, 2529, 2556
SspMI CTAG 8 cut(s) 75, 861, 1020, 1079, 1934, 2036, 2097, 2390
StuI AGGCCT 1 cut(s) 2285
StyD4I CCNGG 7 cut(s) 271, 789, 1677, 1797, 1858, 2145, 2240
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 9 cut(s) 260, 804, 934, 953, 1391, 1498, 1712, 1944, 2384
TaiI ACGT 3 cut(s) 88, 1626, 2194
TaqI TCGA 6 cut(s) 161, 233, 1939, 2056, 2318, 2457
TaqII GACCGA 1 cut(s) 1143
TatI WGTACW 2 cut(s) 2099, 2469
TfiI GAWTC 7 cut(s) 196, 846, 1193, 1930, 2018, 2459, 2569
Tru1I TTAA 3 cut(s) 561, 657, 2217
Tru9I TTAA 3 cut(s) 561, 657, 2217
TscAI CASTG 4 cut(s) 382, 1285, 1348, 1414
TseFI GTSAC 4 cut(s) 86, 263, 587, 2151
TseI GCWGC 6 cut(s) 996, 999, 1354, 1976, 2311, 2451
Tsp45I GTSAC 4 cut(s) 86, 263, 587, 2151
TspDTI ATGAA 5 cut(s) 60, 363, 918, 1683, 2273
TspGWI ACGGA 3 cut(s) 1175, 1913, 2572
TspRI CASTG 4 cut(s) 382, 1285, 1348, 1414
Van91I CCANNNNNTGG 1 cut(s) 1986
VneI GTGCAC 1 cut(s) 2488
VpaK11BI GGWCC 8 cut(s) 14, 321, 986, 1075, 1126, 1310, 1795, 2143
XapI RAATTY 5 cut(s) 696, 1283, 1513, 1523, 2427
XbaI TCTAGA 2 cut(s) 1933, 2035
XceI RCATGY 6 cut(s) 158, 1399, 2078, 2185, 2488, 2496
XhoI CTCGAG 2 cut(s) 160, 232
XmiI GTMKAC 2 cut(s) 715, 1059
XmnI GAANNNNTTC 2 cut(s) 636, 1236
XspI CTAG 8 cut(s) 75, 861, 1020, 1079, 1934, 2036, 2097, 2390
ZrmI AGTACT 1 cut(s) 2101
Zsp2I ATGCAT 1 cut(s) 2187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.