MD11G1149900.v1.1

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003793
Physical Location & Seq
Forward (+)
14235400 .. 14240807
5408 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1149900.v1.1.491

Sequence Viewer

Length: 2580 bp
ATGGCTATTGCTGGTCTACACAATGCTTCTGTGCTTGAGCCTTCTTTCCTTAGAGACTCTCAATCTCATACATCTAGATGGATACGTGATGAAGGGAGGGTGAGTTCCCGCGCATCCTCCCTACTACAAAGGTGGCGGGAGCTTGAGGGTGAGCATGTGGTGAGTCGTGCCCAAGAGAGAGCTAGTGAGGGGTTGCTTGTACAAAGGAGTGATAGGTTGATTGATGAGATCTCGAGGGAAGACACTACTGAAGACCACGGTAGTGACCACGCAGGTGATTTGGAGGATGTGAGTGTGGGCGAGAGTGAGTGCGGATTATGGTCCCGGGGTCAAATTGGGTCATCAAATGAACATGAGGAGTGCAGTAATATTAGCAGTGAGCATTCTGATTTTGGGGAGGTTGAAAGGGGGAGGGTGAGGCAAGTTTTTAGGGAATGGATGAATTGTGGTGTGGCTGAATATACGTCAAATGGTTCTCATATGAATAATAGTTCAAGGGCAGAGTGGCTCGGTGAAACTGAACAGGAAAGGGTGAGAATTGTAAGGGAGTGGGTGCGAATTAACAGCCAGCAGAGAGGTGCCTCGGGTGACAATAGAGGAGAACAACCTGCTGAAATTGGTAATCAAATTGAAAGAGTTCGTGATGGATTGATTGTTAACCAAACTGAAGGCAGGTCTGAGCATACACGAAGGGGAATTCGTAAGTTATGTGGTAGACAGGCTATGCTGGATATGGTAAAGAAGGCTGAGAGGGAAAGACAAAGTGAACTTCAAGTCTTGTTGGAGCACCGGGCTGTATCACAGTTTGCTCACCGCAACCGCATTCAGTCATTGCTTAAAGGAAGATTCTTACGAAATGCTAGAGTGATTGAGAACGAGAGACCTACTTCCTTGGCTGAAAATGAATTAGGCTTATTGAGACAAAGGCACACAGTCTCTGGTCTTAGGGACGGTTTTTGCTCCAGATTGGATAGTTCTGTATCCGGTCAAGTAAGCAGCAGCCACTCTGAGACATCCTGTAGTGTTTCTAATGGTAGTAGAAATGGGGATGGTCAAGCAGACAACCCACGTGAGGTCCTAGCCGGGTCTTCTGAACGATCTGCCGCTAATGACGAGGCAAGTGAGGATCGAGAACATGATAGCTGTGGAATATCAGATTGCAGGGATTTGGGTGGCAACACCATTCAAGATATAGATTCAGATGAATCTAATGCTCCTGTAGAGGGTTGGCAACACCATTCAAGAGACTGGCAGTGCTCAAGTATCACTGAATTTATCGAAAGGAGGGATGTTACTGATTATAACATGATTGGGGACTTACAGGCAACCACTTCTGTTGAGCAGCCCCAAGAAATTTTGCAAAATGATGCTAGTGGACACAGTAACACGCGAAAAGTCAGTCATATATCTAATGAGCAGTCTGAGCCAAGTGGGGAGGCAAGTGTAATAGGTGAACAGTCTGATCATATATATAATTTACTAGACAGCGTGCCTGGGGATGTAAATTTTCAAGAATTTACTTCCCAGGTGCAACAGTGGCAGGATCGAGTTTCAGAAAATGCTGGAAGGGACTGGCAACAACCTGTTGTTGAGTATAATGACTTGAGAGAAAATGTTGGGGAAGATACAACTAGGGATCAGCAGGAAACAACTGCTTATGAATGGTCCCAGGAGTTGTTGGAGAGCGAAGATAGAGAAAACAGTCATCTTGAAGTTCCTGAAGTGTGGCACGAGGAAAGTGGTTTTCAGGAGGCTGTTCAAAGTTGGTTAGAAGAGCCATCGGACCAGGATGTTGATCCGGTCAGGCAGACTGACGCATTTTATTTTCCGGATGATGATAATGCTCCTGGCACCGAGCTAAGGGAACTCCTAAGCAGGAGAAGGGTCTCTAATCTTCTTAGTAGTGGTTTCCGTCAGAATCTAGATCGACTGTTACAATCATATGTAGAAAGGCAAAGTCACGCTGCCATTGACTGGGAGCTGGATGATACATCACCTTCTCCTGAACCTGCAGAACAGGATCTAGAGCAGACAGGTGCATATCAAAATGGGGGTCACGTGGATGCTGTTGAGAGTCGTAGTCCTAGTACTGCTCTACGATCACAGCAAATACCTTCCTTGCCACTTTGGGACCAGGAGTCACACTCTGATAATTGGCCACAGCATGACATGCATCAACATAACGGAATTGACTGGGAGGTAATTAACGATATGAGGATTGACATGGCCAGGCTATACCAGAGGATGAATAACTTGCAAAGAATGATGGAGGCCTGCATGGATATGCAACTTGAGTTGCAGCGCTCGATAAGACAAGAAGTTTCTGATGCCCTGAATAGATCAGCTGGTTCACGAGGGGTGTGTGAAGACGGTCTGCTAGAAGATGGGTCTAAATGGGATCATGTAAGGAAAGGAATTTGCTGTATATGTTGTGAAAGCAGCATCGATTCTTTATTGTACAGATGTGGCCACATTTGCGCGTGTTCAAAATGTGCTACTGAATTGGTTGAGACTAGCGGAAAATGTCCGATGTGTAGAGCACCCGTGATTGAGGTGATTCGTGTTTATTCCGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

860

Amino Acids

97.19

Weight (kDa)

4.78

Isoelectric Point (pI)

61.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 805 - 851 2.7e-10 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1302
AarI CACCTGC 1 cut(s) 263
Acc36I ACCTGC 4 cut(s) 263, 616, 663, 2017
AccB1I GGYRCC 2 cut(s) 578, 1850
AccB7I CCANNNNNTGG 1 cut(s) 1974
AccI GTMKAC 2 cut(s) 16, 715
AccII CGCG 3 cut(s) 111, 1390, 2480
AccIII TCCGGA 1 cut(s) 1828
AciI CCGC 7 cut(s) 109, 136, 312, 814, 820, 1104, 2517
AclWI GGATC 6 cut(s) 1134, 1551, 1644, 1790, 2028, 2406
AcoI YGGCCR 3 cut(s) 2156, 2226, 2467
AcsI RAATTY 6 cut(s) 696, 1271, 1353, 1504, 1514, 2415
AcuI CTGAAG 3 cut(s) 270, 687, 1740
AcvI CACGTG 2 cut(s) 1070, 2059
AfaI GTAC 3 cut(s) 201, 2089, 2459
AfeI AGCGCT 1 cut(s) 2303
AfiI CCNNNNNNNGG 4 cut(s) 1072, 1223, 1860, 1974
AhdI GACNNNNNGTC 1 cut(s) 2137
AjnI CCWGG 7 cut(s) 1492, 1524, 1668, 1785, 1846, 2133, 2228
AleI CACNNNNGTG 2 cut(s) 261, 273
AloI GAACNNNNNNTCC 2 cut(s) 88, 120
AluBI AGCT 6 cut(s) 142, 182, 1143, 1858, 1981, 2345
AluI AGCT 6 cut(s) 142, 182, 1143, 1858, 1981, 2345
Alw21I GWGCWC 3 cut(s) 789, 1259, 2542
Alw26I GTCTC 8 cut(s) 48, 874, 913, 940, 1004, 1239, 1891, 2504
AlwI GGATC 6 cut(s) 1134, 1551, 1644, 1790, 2028, 2406
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 3 cut(s) 232, 324, 583
Aor13HI TCCGGA 1 cut(s) 1828
Aor51HI AGCGCT 1 cut(s) 2303
AoxI GGCC 4 cut(s) 2156, 2226, 2271, 2467
ApeKI GCWGC 6 cut(s) 996, 999, 1342, 1964, 2299, 2439
ApoI RAATTY 6 cut(s) 696, 1271, 1353, 1504, 1514, 2415
ArsI GACNNNNNNTTYG 4 cut(s) 1384, 1416, 2038, 2070
Asp700I GAANNNNTTC 1 cut(s) 636
AspLEI GCGC 3 cut(s) 113, 2304, 2480
AspS9I GGNCC 5 cut(s) 321, 1075, 1665, 1783, 2131
AsuC2I CCSGG 4 cut(s) 325, 326, 791, 1084
AvaI CYCGRG 3 cut(s) 232, 324, 583
AvaII GGWCC 5 cut(s) 321, 1075, 1665, 1783, 2131
BaeGI GKGCMC 1 cut(s) 172
BalI TGGCCA 3 cut(s) 2158, 2228, 2469
BanI GGYRCC 2 cut(s) 578, 1850
BauI CACGAG 2 cut(s) 1730, 2352
BbrPI CACGTG 2 cut(s) 1070, 2059
BbsI GAAGAC 4 cut(s) 246, 258, 1080, 2373
Bbv12I GWGCWC 3 cut(s) 789, 1259, 2542
BbvI GCAGC 6 cut(s) 1008, 1011, 1354, 1951, 2311, 2451
BccI CCATC 6 cut(s) 72, 638, 1043, 1786, 2260, 2378
BciT130I CCWGG 7 cut(s) 1494, 1526, 1670, 1787, 1848, 2135, 2230
BciVI GTATCC 2 cut(s) 75, 991
BclI TGATCA 1 cut(s) 1462
BcnI CCSGG 4 cut(s) 325, 326, 791, 1084
BcoDI GTCTC 8 cut(s) 48, 874, 913, 940, 1004, 1239, 1891, 2504
BfmI CTRYAG 3 cut(s) 1018, 1218, 2010
BfoI RGCGCY 1 cut(s) 2305
BfuAI ACCTGC 4 cut(s) 263, 616, 663, 2017
BfuI GTATCC 2 cut(s) 75, 991
BglII AGATCT 1 cut(s) 228
BisI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1965, 2300, 2440
BlsI GCNGC 7 cut(s) 998, 1001, 1105, 1344, 1966, 2301, 2441
BmcAI AGTACT 1 cut(s) 2089
Bme18I GGWCC 5 cut(s) 321, 1075, 1665, 1783, 2131
BmeRI GACNNNNNGTC 1 cut(s) 2137
BmeT110I CYCGRG 3 cut(s) 232, 324, 583
BmgT120I GGNCC 5 cut(s) 321, 1075, 1665, 1783, 2131
BmiI GGNNCC 5 cut(s) 323, 580, 1667, 1852, 2132
BmrI ACTGGG 2 cut(s) 1984, 2203
BmsI GCATC 6 cut(s) 122, 1357, 2053, 2182, 2317, 2451
BmuI ACTGGG 2 cut(s) 1984, 2203
BpiI GAAGAC 4 cut(s) 246, 258, 1080, 2373
BplI GAGNNNNNCTC 2 cut(s) 2129, 2161
BpmI CTGGAG 1 cut(s) 946
Bpu10I CCTNAGC 2 cut(s) 1859, 1871
BpuEI CTTGAG 5 cut(s) 56, 164, 1243, 1624, 2312
BpuMI CCSGG 4 cut(s) 325, 326, 791, 1084
Bsa29I ATCGAT 1 cut(s) 2445
BsaAI YACGTR 3 cut(s) 86, 1070, 2059
BsaBI GATNNNNATC 1 cut(s) 1794
BsaI GGTCTC 2 cut(s) 874, 1891
BsaJI CCNNGG 8 cut(s) 256, 324, 325, 582, 891, 1493, 1524, 1668
BsaWI WCCGGW 3 cut(s) 983, 1798, 1828
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 4 cut(s) 1072, 1223, 1860, 1974
Bse1I ACTGG 4 cut(s) 1253, 1577, 1979, 2198
Bse3DI GCAATG 1 cut(s) 830
Bse8I GATNNNNATC 1 cut(s) 1794
BseAI TCCGGA 1 cut(s) 1828
BseBI CCWGG 7 cut(s) 1494, 1526, 1670, 1787, 1848, 2135, 2230
BseCI ATCGAT 1 cut(s) 2445
BseDI CCNNGG 8 cut(s) 256, 324, 325, 582, 891, 1493, 1524, 1668
BseJI GATNNNNATC 1 cut(s) 1794
BseLI CCNNNNNNNGG 4 cut(s) 1072, 1223, 1860, 1974
BseMI GCAATG 1 cut(s) 830
BseMII CTCAG 4 cut(s) 669, 738, 999, 1413
BseNI ACTGG 4 cut(s) 1253, 1577, 1979, 2198
BseRI GAGGAG 2 cut(s) 371, 612
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 6 cut(s) 1008, 1011, 1354, 1951, 2311, 2451
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 3 cut(s) 111, 1390, 2480
BshFI GGCC 4 cut(s) 2158, 2228, 2273, 2469
BshNI GGYRCC 2 cut(s) 578, 1850
BshVI ATCGAT 1 cut(s) 2445
BsiHKAI GWGCWC 3 cut(s) 789, 1259, 2542
BsiHKCI CYCGRG 3 cut(s) 232, 324, 583
BsiSI CCGG 6 cut(s) 325, 790, 984, 1083, 1799, 1829
BslFI GGGAC 6 cut(s) 307, 962, 1328, 1583, 1651, 2144
BslI CCNNNNNNNGG 4 cut(s) 1072, 1223, 1860, 1974
BsmAI GTCTC 8 cut(s) 48, 874, 913, 940, 1004, 1239, 1891, 2504
BsmFI GGGAC 6 cut(s) 307, 962, 1328, 1583, 1651, 2144
BsmI GAATGC 2 cut(s) 382, 822
BsnI GGCC 4 cut(s) 2158, 2228, 2273, 2469
Bso31I GGTCTC 2 cut(s) 874, 1891
BsoBI CYCGRG 3 cut(s) 232, 324, 583
Bsp1286I GDGCHC 4 cut(s) 172, 789, 1259, 2542
Bsp13I TCCGGA 1 cut(s) 1828
Bsp1407I TGTACA 2 cut(s) 199, 2457
BspACI CCGC 7 cut(s) 109, 136, 312, 814, 820, 1104, 2517
BspANI GGCC 4 cut(s) 2158, 2228, 2273, 2469
BspCNI CTCAG 4 cut(s) 670, 739, 1000, 1414
BspDI ATCGAT 1 cut(s) 2445
BspEI TCCGGA 1 cut(s) 1828
BspFNI CGCG 3 cut(s) 111, 1390, 2480
BspLI GGNNCC 5 cut(s) 323, 580, 1667, 1852, 2132
BspMAI CTGCAG 1 cut(s) 2014
BspMI ACCTGC 4 cut(s) 263, 616, 663, 2017
BspPI GGATC 6 cut(s) 1134, 1551, 1644, 1790, 2028, 2406
BspQI GCTCTTC 1 cut(s) 1767
BspT107I GGYRCC 2 cut(s) 578, 1850
BspTNI GGTCTC 2 cut(s) 874, 1891
BsrDI GCAATG 1 cut(s) 830
BsrGI TGTACA 2 cut(s) 199, 2457
BsrI ACTGG 4 cut(s) 1253, 1577, 1979, 2198
BssECI CCNNGG 8 cut(s) 256, 324, 325, 582, 891, 1493, 1524, 1668
BssSI CACGAG 2 cut(s) 1730, 2352
BssT1I CCWWGG 1 cut(s) 891
Bst2BI CACGAG 2 cut(s) 1730, 2352
Bst2UI CCWGG 7 cut(s) 1494, 1526, 1670, 1787, 1848, 2135, 2230
Bst6I CTCTTC 1 cut(s) 1767
BstAPI GCANNNNNTGC 1 cut(s) 2170
BstAUI TGTACA 2 cut(s) 199, 2457
BstBAI YACGTR 3 cut(s) 86, 1070, 2059
BstC8I GCNNGC 3 cut(s) 569, 1490, 2275
BstDEI CTNAG 9 cut(s) 50, 678, 747, 944, 1008, 1422, 1859, 1871, 1898
BstDSI CCRYGG 1 cut(s) 256
BstFNI CGCG 3 cut(s) 111, 1390, 2480
BstH2I RGCGCY 1 cut(s) 2305
BstHHI GCGC 3 cut(s) 113, 2304, 2480
BstMAI GTCTC 8 cut(s) 48, 874, 913, 940, 1004, 1239, 1891, 2504
BstMWI GCNNNNNNNGC 4 cut(s) 1423, 1537, 2170, 2475
BstNI CCWGG 7 cut(s) 1494, 1526, 1670, 1787, 1848, 2135, 2230
BstNSI RCATGY 2 cut(s) 158, 2173
BstSFI CTRYAG 3 cut(s) 1018, 1218, 2010
BstSLI GKGCMC 1 cut(s) 172
BstUI CGCG 3 cut(s) 111, 1390, 2480
BstV1I GCAGC 6 cut(s) 1008, 1011, 1354, 1951, 2311, 2451
BstV2I GAAGAC 4 cut(s) 246, 258, 1080, 2373
BstX2I RGATCY 2 cut(s) 228, 2020
BstYI RGATCY 2 cut(s) 228, 2020
Bsu15I ATCGAT 1 cut(s) 2445
BsuI GTATCC 2 cut(s) 75, 991
BsuRI GGCC 4 cut(s) 2158, 2228, 2273, 2469
BsuTUI ATCGAT 1 cut(s) 2445
BtgI CCRYGG 1 cut(s) 256
BtsI GCAGTG 2 cut(s) 382, 1259
BtsIMutI CAGTG 4 cut(s) 382, 1259, 1266, 1541
BveI ACCTGC 4 cut(s) 263, 616, 663, 2017
Cac8I GCNNGC 3 cut(s) 569, 1490, 2275
CaiI CAGNNNCTG 1 cut(s) 938
CfoI GCGC 3 cut(s) 113, 2304, 2480
Cfr13I GGNCC 5 cut(s) 321, 1075, 1665, 1783, 2131
Cfr9I CCCGGG 1 cut(s) 324
ClaI ATCGAT 1 cut(s) 2445
CseI GACGC 1 cut(s) 1823
Csp6I GTAC 3 cut(s) 200, 2088, 2458
CspCI CAANNNNNGTGG 2 cut(s) 1318, 1353
CviAII CATG 9 cut(s) 155, 353, 1136, 1306, 2165, 2170, 2224, 2278, 2402
CviQI GTAC 3 cut(s) 200, 2088, 2458
DdeI CTNAG 9 cut(s) 50, 678, 747, 944, 1008, 1422, 1859, 1871, 1898
DriI GACNNNNNGTC 1 cut(s) 2137
EaeI YGGCCR 3 cut(s) 2156, 2226, 2467
Eam1104I CTCTTC 1 cut(s) 1767
Eam1105I GACNNNNNGTC 1 cut(s) 2137
EarI CTCTTC 1 cut(s) 1767
Eco130I CCWWGG 1 cut(s) 891
Eco147I AGGCCT 1 cut(s) 2273
Eco31I GGTCTC 2 cut(s) 874, 1891
Eco47I GGWCC 5 cut(s) 321, 1075, 1665, 1783, 2131
Eco47III AGCGCT 1 cut(s) 2303
Eco57I CTGAAG 3 cut(s) 270, 687, 1740
Eco72I CACGTG 2 cut(s) 1070, 2059
Eco88I CYCGRG 3 cut(s) 232, 324, 583
EcoO109I RGGNCCY 1 cut(s) 1075
EcoRI GAATTC 1 cut(s) 696
EcoRII CCWGG 7 cut(s) 1492, 1524, 1668, 1785, 1846, 2133, 2228
EcoT14I CCWWGG 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 2175
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 9 cut(s) 158, 356, 1139, 1309, 2168, 2173, 2227, 2281, 2405
FaqI GGGAC 6 cut(s) 307, 962, 1328, 1583, 1651, 2144
FatI CATG 9 cut(s) 154, 352, 1135, 1305, 2164, 2169, 2223, 2277, 2401
FauI CCCGC 2 cut(s) 116, 129
FauNDI CATATG 2 cut(s) 480, 1942
FbaI TGATCA 1 cut(s) 1462
FblI GTMKAC 2 cut(s) 16, 715
Fnu4HI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1965, 2300, 2440
Fsp4HI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1965, 2300, 2440
GlaI GCGC 3 cut(s) 112, 2303, 2479
GluI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1965, 2300, 2440
GsuI CTGGAG 1 cut(s) 946
HaeII RGCGCY 1 cut(s) 2305
HaeIII GGCC 4 cut(s) 2158, 2228, 2273, 2469
HapII CCGG 6 cut(s) 325, 790, 984, 1083, 1799, 1829
HgaI GACGC 1 cut(s) 1823
HhaI GCGC 3 cut(s) 113, 2304, 2480
Hin1II CATG 9 cut(s) 158, 356, 1139, 1309, 2168, 2173, 2227, 2281, 2405
Hin6I GCGC 3 cut(s) 111, 2302, 2478
HinP1I GCGC 3 cut(s) 111, 2302, 2478
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 6 cut(s) 325, 790, 984, 1083, 1799, 1829
Hpy166II GTNNAC 7 cut(s) 17, 658, 716, 767, 1376, 1454, 2351
Hpy8I GTNNAC 7 cut(s) 17, 658, 716, 767, 1376, 1454, 2351
HpyAV CCTTC 9 cut(s) 51, 86, 662, 684, 736, 1560, 1875, 2007, 2124
HpyCH4IV ACGT 4 cut(s) 85, 464, 1069, 2058
HpyF10VI GCNNNNNNNGC 4 cut(s) 1423, 1537, 2170, 2475
HpyF3I CTNAG 9 cut(s) 50, 678, 747, 944, 1008, 1422, 1859, 1871, 1898
HpySE526I ACGT 4 cut(s) 85, 464, 1069, 2058
Hsp92II CATG 9 cut(s) 158, 356, 1139, 1309, 2168, 2173, 2227, 2281, 2405
HspAI GCGC 3 cut(s) 111, 2302, 2478
Kpn2I TCCGGA 1 cut(s) 1828
Ksp22I TGATCA 1 cut(s) 1462
KspAI GTTAAC 1 cut(s) 658
LguI GCTCTTC 1 cut(s) 1767
LmnI GCTCC 6 cut(s) 139, 784, 965, 1219, 1849, 1978
Lsp1109I GCAGC 6 cut(s) 1008, 1011, 1354, 1951, 2311, 2451
LweI GCATC 6 cut(s) 122, 1357, 2053, 2182, 2317, 2451
MaeII ACGT 4 cut(s) 85, 464, 1069, 2058
MaeIII GTNAC 8 cut(s) 263, 587, 1291, 1382, 1932, 1958, 2054, 2139
MflI RGATCY 2 cut(s) 228, 2020
MhlI GDGCHC 4 cut(s) 172, 789, 1259, 2542
MlsI TGGCCA 3 cut(s) 2158, 2228, 2469
MluNI TGGCCA 3 cut(s) 2158, 2228, 2469
MlyI GAGTC 4 cut(s) 50, 172, 2083, 2147
MmeI TCCRAC 2 cut(s) 762, 1659
Mox20I TGGCCA 3 cut(s) 2158, 2228, 2469
Mph1103I ATGCAT 1 cut(s) 2175
MroI TCCGGA 1 cut(s) 1828
MroXI GAANNNNTTC 1 cut(s) 636
MscI TGGCCA 3 cut(s) 2158, 2228, 2469
MseI TTAA 4 cut(s) 561, 657, 837, 2205
MslI CAYNNNNRTG 5 cut(s) 76, 261, 273, 2061, 2282
Msp20I TGGCCA 3 cut(s) 2158, 2228, 2469
MspA1I CMGCKG 1 cut(s) 2345
MspI CCGG 6 cut(s) 325, 790, 984, 1083, 1799, 1829
Mva1269I GAATGC 2 cut(s) 382, 822
MvaI CCWGG 7 cut(s) 1494, 1526, 1670, 1787, 1848, 2135, 2230
MvnI CGCG 3 cut(s) 111, 1390, 2480
MwoI GCNNNNNNNGC 4 cut(s) 1423, 1537, 2170, 2475
NciI CCSGG 4 cut(s) 325, 326, 791, 1084
NdeI CATATG 2 cut(s) 480, 1942
NlaIII CATG 9 cut(s) 158, 356, 1139, 1309, 2168, 2173, 2227, 2281, 2405
NlaIV GGNNCC 5 cut(s) 323, 580, 1667, 1852, 2132
NmuCI GTSAC 5 cut(s) 263, 587, 1958, 2054, 2139
NsiI ATGCAT 1 cut(s) 2175
NspI RCATGY 2 cut(s) 158, 2173
OliI CACNNNNGTG 2 cut(s) 261, 273
PaeR7I CTCGAG 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 263
PceI AGGCCT 1 cut(s) 2273
PciSI GCTCTTC 1 cut(s) 1767
PctI GAATGC 2 cut(s) 382, 822
PdmI GAANNNNTTC 1 cut(s) 636
PfeI GAWTC 6 cut(s) 846, 1196, 1205, 1918, 2447, 2557
PflMI CCANNNNNTGG 1 cut(s) 1974
PkrI GCNGC 7 cut(s) 998, 1001, 1105, 1344, 1966, 2301, 2441
PleI GAGTC 4 cut(s) 50, 171, 2082, 2146
PmaCI CACGTG 2 cut(s) 1070, 2059
PmlI CACGTG 2 cut(s) 1070, 2059
PpsI GAGTC 4 cut(s) 50, 171, 2082, 2146
Ppu21I YACGTR 3 cut(s) 86, 1070, 2059
PpuMI RGGWCCY 1 cut(s) 1075
PsiI TTATAA 1 cut(s) 1302
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 7 cut(s) 1492, 1524, 1668, 1785, 1846, 2133, 2228
PspCI CACGTG 2 cut(s) 1070, 2059
PspGI CCWGG 7 cut(s) 1492, 1524, 1668, 1785, 1846, 2133, 2228
PspN4I GGNNCC 5 cut(s) 323, 580, 1667, 1852, 2132
PspPI GGNCC 5 cut(s) 321, 1075, 1665, 1783, 2131
PspPPI RGGWCCY 1 cut(s) 1075
PstI CTGCAG 1 cut(s) 2014
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 2 cut(s) 228, 2020
PvuII CAGCTG 1 cut(s) 2345
RsaI GTAC 3 cut(s) 201, 2089, 2459
RsaNI GTAC 3 cut(s) 200, 2088, 2458
RseI CAYNNNNRTG 5 cut(s) 76, 261, 273, 2061, 2282
SapI GCTCTTC 1 cut(s) 1767
SaqAI TTAA 4 cut(s) 561, 657, 837, 2205
SatI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1965, 2300, 2440
Sau96I GGNCC 5 cut(s) 321, 1075, 1665, 1783, 2131
ScaI AGTACT 1 cut(s) 2089
SchI GAGTC 4 cut(s) 50, 172, 2083, 2147
SduI GDGCHC 4 cut(s) 172, 789, 1259, 2542
SfaNI GCATC 6 cut(s) 122, 1357, 2053, 2182, 2317, 2451
SfcI CTRYAG 3 cut(s) 1018, 1218, 2010
Sfr274I CTCGAG 1 cut(s) 232
SinI GGWCC 5 cut(s) 321, 1075, 1665, 1783, 2131
SlaI CTCGAG 1 cut(s) 232
SmaI CCCGGG 1 cut(s) 326
SmiMI CAYNNNNRTG 5 cut(s) 76, 261, 273, 2061, 2282
SmlI CTYRAG 6 cut(s) 35, 143, 232, 1258, 1603, 2291
SmoI CTYRAG 6 cut(s) 35, 143, 232, 1258, 1603, 2291
SseBI AGGCCT 1 cut(s) 2273
SsiI CCGC 7 cut(s) 109, 136, 312, 814, 820, 1104, 2517
SspI AATATT 1 cut(s) 370
StuI AGGCCT 1 cut(s) 2273
StyI CCWWGG 1 cut(s) 891
TaiI ACGT 4 cut(s) 88, 467, 1072, 2061
TaqI TCGA 7 cut(s) 233, 1129, 1278, 1546, 1927, 2306, 2445
TatI WGTACW 3 cut(s) 199, 2087, 2457
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 6 cut(s) 846, 1196, 1205, 1918, 2447, 2557
Tru1I TTAA 4 cut(s) 561, 657, 837, 2205
Tru9I TTAA 4 cut(s) 561, 657, 837, 2205
TscAI CASTG 4 cut(s) 382, 1259, 1273, 1541
TseFI GTSAC 5 cut(s) 263, 587, 1958, 2054, 2139
TseI GCWGC 6 cut(s) 996, 999, 1342, 1964, 2299, 2439
Tsp45I GTSAC 5 cut(s) 263, 587, 1958, 2054, 2139
TspDTI ATGAA 8 cut(s) 105, 363, 455, 497, 918, 1218, 1674, 2261
TspGWI ACGGA 3 cut(s) 1901, 2199, 2560
TspMI CCCGGG 1 cut(s) 324
TspRI CASTG 4 cut(s) 382, 1259, 1273, 1541
Van91I CCANNNNNTGG 1 cut(s) 1974
VpaK11BI GGWCC 5 cut(s) 321, 1075, 1665, 1783, 2131
XapI RAATTY 6 cut(s) 696, 1271, 1353, 1504, 1514, 2415
XbaI TCTAGA 3 cut(s) 74, 1921, 2023
XceI RCATGY 2 cut(s) 158, 2173
XhoI CTCGAG 1 cut(s) 232
XmaI CCCGGG 1 cut(s) 324
XmiI GTMKAC 2 cut(s) 16, 715
XmnI GAANNNNTTC 1 cut(s) 636
ZrmI AGTACT 1 cut(s) 2089
Zsp2I ATGCAT 1 cut(s) 2175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.