Rh5AG370300

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003793
Physical Location & Seq
Reverse (-)
62700887 .. 62706149
5263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG370300.1

Sequence Viewer

Length: 2529 bp
ATGGCTATTGCTGGTCTACAAAATGTTTCTGTGCTCGATTCTTCTTTCCTTAGAAGCTCTCAATCTGGTGCATCTAGAAGGGTGGACAATGAAGGGAGGGCACACTCCCGGGCTTCCTCTCTGCTACAAATGTGGCGAGAGCTTGAGGATGAGCATATGGTGAGTCGTCCCCTCGAGAGAGTTGGTGAAAGGTTGTTCCAACAAAGGATTGATGGAGTTGGTAGCGAGAATACAGGTGACCTGGATGATGCGAGTGTGGCTGAGAGTGAGTGCAGGACATGGTCCCAAGGTCAAATTGGCTCATCCCCTGAACATGATGAATATAGTAATTTTAGCAGTGAGCACTCTTCTGATTTTGGGGACGGTGAAAGGGGGAGGGTGAGACAAATTTTTCAGGAATGGATGAACTGTGGTGCAAGTGAATGTGCATCAAATGTTTCTCATATGAATAACAGTTCAAGGGCAGAGTTGCTTGGTGAAACTGAACAGGAGAGAGTGAGAATTATAAGGGAGTGGGTACGAATGAACAGCCAGCAAAGAGTTGCTTCTGGTGACAATGGAGAAGAACAACCTGCTGAGTTTGGTAATCAAATTGCACGAGTTCGTGATGGATTGGTTGTTAACCAAAGTGGAGGCCGGTCTGAGCATAACCGGAGGGGAATTCGTAAATTATGTGGGAGACAAGCTCTACTGGATATGTTAAAGAAGGCTGAGAGGGAAAGGCAAATAGAACTTCAAGTCTTGTCGGAGCACCAGCCCGTAACACGCTTTGCTCACCGCAACCGCATTCAGTCATTGCTTCGAGGAAGATTTTTACGAAATGGTAGAGCCATTGAGAACGAGAGACCCATTTCGATGGCTGAAACTGAATTGGGCTTATTGAGGCAAAGACATACAGTCTCTGGTCTGAGGGATGGTTTTGACTCCAAATTGGATAGTCCTGTTGGTGGTCAAGTAGGCAGCTGCCATTCTGAGCCATCTTCAAGCAATTCTAATGGTATCAGAAGTGGCCATACTCAACCGAACAATTCACATGAAGTCGTTAGTGGTTCTTTTGAGCAGTCTGAGGCTAATGATGTTGCAAGTAATGACCGAGGACACAATAGTTGTGGAACATCAGCTGCTAGGGCTGATTTGGGTGGAAACACCATTCAGGATATAGATTCACATGAATCTAATGCTCCCATAGAACATGGGCAAGAAGAGATTCCTGATAATGTTGTAAGTGGCTGGCAATGCTCATCCTCTATTGAGATTGTTGAAAGTAGAGATGGTGCTTGTCAAAACATGATTGGGAACTTACTTACAACCACTGCTGTTGAGCAGTCCCAAGAAACTTTGCACAATGATGCTAATGACCACAGTAACATTCAAGGAGCCAGTGATTTATCTAATGAGCAATCTGAGCTTGATGGGGATGAAAGTGTCATCCACGAACTCAGTCATGCAGATGGCAATATGCTTGAGGATGTAAATTTTCAAGAGTCCAATTCACAGCCACACCAATGGCTGGATCAAGTTTCAGAAAATGAGGAAGGAGACCAGGTGCAAGCTGATGAGTATAATAGTTTGATAGAAAATGTCGTGGAACATACAGGTGAAGATCAGCAGGAAACTGCTGGTTATGAATGGTCCCAGGAGTTGGTAGAGAACGAAGATAGAGAAGATGATCAACTCCAAGAAGTCCCTGAAGTGTGGCACGATGAAAGTGGTTTTCAAGAGGCTGTTCAAAGTTGGTTAGAAGGGCCATCCAGCCAGGATTCCGATTCAGTCAGGCAGGCTGATACCTTTTACTTTCCTGATGATGATAATGCTCATAATACAGAGCTAAGGGAACTTCTAAGCAGGAGAAGGGTCTCTAATCTTCTGAGCAGTGGTTTCCGTCAGAACCTTGATCAACTCATACAGTCATATGTGGAAAGGCAAGGTCATGCCGCCATTGACTGGGATGAAGCATCAGCTTATCCTGAATTGACTGAAGATATGGAGCAGACGTATCAGAATGAAGTTCAAGTGAATGCTGTTGAGAGTCCTCACCTTGCTCTACCACCGCAGCCAATACCTTCCTCTCCGCTGTGGCACCAGGAGTCACCCCATGATAATTGGCCACGACATGACATGCATCAGCGCTTTGGAATTGACTGGGAGATGGTGAACGATATGAGGATTGACATGGCTAGGCTGCAACAGAGGATGAACAACTTGCAAAGGATGTTGGAGGCCTGCATGGATATGCAACTTGAGTTGCAACGATCGATAAGACAAGAAGTTTCTGATGCCTTGAATCGGTCAGCCGGTTCACAAGGGGTGTGTGAAGATGGTCTCCTAGATGATGGGTCTAAATGGGATAATGTAAGGAAAGGAATTTGCTGTATATGTTGTGTTAGCAGTATTGATTCTTTACTATACAGATGTGGTCACATGTGCACATGTTCAAAATGCGCTACCAAATTGGTTGAGAGTAGCGGAAAATGTCCGATGTGCAGAGCACCCGCAGTTGAGGTGATCCGGGCTTATTCTGTATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

842

Amino Acids

94.35

Weight (kDa)

4.69

Isoelectric Point (pI)

59.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 788 - 832 7.5e-10 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 506, 2527
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 2079
AccB7I CCANNNNNTGG 3 cut(s) 1510, 1642, 1944
AccI GTMKAC 1 cut(s) 16
AciI CCGC 7 cut(s) 778, 784, 1935, 2051, 2072, 2466, 2493
AclWI GGATC 2 cut(s) 1521, 2500
AcoI YGGCCR 2 cut(s) 1009, 2105
AcsI RAATTY 4 cut(s) 387, 660, 1474, 2364
AcuI CTGAAG 2 cut(s) 1710, 1998
AfaI GTAC 1 cut(s) 519
AfeI AGCGCT 1 cut(s) 2129
AfiI CCNNNNNNNGG 5 cut(s) 947, 1510, 1642, 1944, 2286
AflIII ACRYGT 2 cut(s) 2421, 2429
AhdI GACNNNNNGTC 1 cut(s) 896
AjnI CCWGG 5 cut(s) 240, 1542, 1635, 1755, 2082
AluBI AGCT 9 cut(s) 57, 142, 686, 963, 1121, 1408, 1553, 1828, 1961
AluI AGCT 9 cut(s) 57, 142, 686, 963, 1121, 1408, 1553, 1828, 1961
Alw21I GWGCWC 5 cut(s) 36, 345, 753, 2429, 2491
Alw26I GTCTC 7 cut(s) 376, 673, 838, 904, 1533, 1861, 2327
Alw44I GTGCAC 1 cut(s) 2425
AlwI GGATC 2 cut(s) 1521, 2500
AlwNI CAGNNNCTG 1 cut(s) 902
Ama87I CYCGRG 2 cut(s) 108, 173
Aor51HI AGCGCT 1 cut(s) 2129
AoxI GGCC 5 cut(s) 634, 1009, 1745, 2105, 2220
ApaLI GTGCAC 1 cut(s) 2425
ApeKI GCWGC 5 cut(s) 960, 963, 1121, 2053, 2182
ApoI RAATTY 4 cut(s) 387, 660, 1474, 2364
Asp700I GAANNNNTTC 1 cut(s) 1206
AspLEI GCGC 2 cut(s) 2130, 2444
AspS9I GGNCC 3 cut(s) 282, 1632, 1745
AsuC2I CCSGG 3 cut(s) 109, 110, 2510
AvaI CYCGRG 2 cut(s) 108, 173
AvaII GGWCC 2 cut(s) 282, 1632
BaeGI GKGCMC 2 cut(s) 103, 2429
BalI TGGCCA 2 cut(s) 1011, 2107
BanI GGYRCC 1 cut(s) 2079
BarI GAAGNNNNNNTAC 2 cut(s) 997, 1029
BauI CACGAG 1 cut(s) 597
Bbv12I GWGCWC 5 cut(s) 36, 345, 753, 2429, 2491
BbvI GCAGC 5 cut(s) 950, 972, 1108, 2065, 2169
BciT130I CCWGG 5 cut(s) 242, 1544, 1637, 1757, 2084
BclI TGATCA 2 cut(s) 1669, 1894
BcnI CCSGG 3 cut(s) 109, 110, 2510
BcoDI GTCTC 7 cut(s) 376, 673, 838, 904, 1533, 1861, 2327
BfaI CTAG 4 cut(s) 75, 1125, 2178, 2327
BfoI RGCGCY 1 cut(s) 2131
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 6 cut(s) 961, 964, 1122, 1935, 2054, 2183
BlsI GCNGC 6 cut(s) 962, 965, 1123, 1936, 2055, 2184
Bme1390I CCNGG 8 cut(s) 109, 110, 242, 1544, 1637, 1757, 2084, 2510
Bme18I GGWCC 2 cut(s) 282, 1632
BmeRI GACNNNNNGTC 1 cut(s) 896
BmeT110I CYCGRG 2 cut(s) 108, 173
BmgT120I GGNCC 3 cut(s) 282, 1632, 1745
BmiI GGNNCC 4 cut(s) 284, 1378, 1634, 2081
BmrFI CCNGG 8 cut(s) 109, 110, 242, 1544, 1637, 1757, 2084, 2510
BmrI ACTGGG 2 cut(s) 1954, 2152
BmsI GCATC 7 cut(s) 80, 238, 437, 1339, 1964, 2131, 2266
BmuI ACTGGG 2 cut(s) 1954, 2152
BplI GAGNNNNNCTC 2 cut(s) 670, 702
Bpu10I CCTNAGC 1 cut(s) 1829
BpuEI CTTGAG 3 cut(s) 164, 1484, 2261
BpuMI CCSGG 3 cut(s) 109, 110, 2510
Bsa29I ATCGAT 1 cut(s) 2255
BsaI GGTCTC 4 cut(s) 838, 1533, 1861, 2327
BsaJI CCNNGG 4 cut(s) 108, 286, 1093, 1635
BsaWI WCCGGW 1 cut(s) 651
BsaXI ACNNNNNCTCC 4 cut(s) 1530, 1560, 1979, 2009
Bsc4I CCNNNNNNNGG 5 cut(s) 947, 1510, 1642, 1944, 2286
Bse118I RCCGGY 2 cut(s) 636, 2294
Bse1I ACTGG 4 cut(s) 696, 1380, 1949, 2147
Bse3DI GCAATG 2 cut(s) 794, 1241
BseBI CCWGG 5 cut(s) 242, 1544, 1637, 1757, 2084
BseCI ATCGAT 1 cut(s) 2255
BseDI CCNNGG 4 cut(s) 108, 286, 1093, 1635
BseLI CCNNNNNNNGG 5 cut(s) 947, 1510, 1642, 1944, 2286
BseMI GCAATG 2 cut(s) 794, 1241
BseNI ACTGG 4 cut(s) 696, 1380, 1949, 2147
BseSI GKGCMC 2 cut(s) 103, 2429
BseXI GCAGC 5 cut(s) 950, 972, 1108, 2065, 2169
BsgI GTGCAG 2 cut(s) 292, 2503
Bsh1285I CGRYCG 1 cut(s) 2255
BshFI GGCC 5 cut(s) 636, 1011, 1747, 2107, 2222
BshNI GGYRCC 1 cut(s) 2079
BshVI ATCGAT 1 cut(s) 2255
BsiEI CGRYCG 1 cut(s) 2255
BsiHKAI GWGCWC 5 cut(s) 36, 345, 753, 2429, 2491
BsiHKCI CYCGRG 2 cut(s) 108, 173
BsiSI CCGG 5 cut(s) 109, 637, 652, 2295, 2509
BslFI GGGAC 6 cut(s) 153, 268, 374, 1312, 1618, 1670
BslI CCNNNNNNNGG 5 cut(s) 947, 1510, 1642, 1944, 2286
BsmAI GTCTC 7 cut(s) 376, 673, 838, 904, 1533, 1861, 2327
BsmFI GGGAC 6 cut(s) 153, 268, 374, 1312, 1618, 1670
BsmI GAATGC 2 cut(s) 786, 2023
BsnI GGCC 5 cut(s) 636, 1011, 1747, 2107, 2222
Bso31I GGTCTC 4 cut(s) 838, 1533, 1861, 2327
BsoBI CYCGRG 2 cut(s) 108, 173
Bsp1286I GDGCHC 6 cut(s) 36, 103, 345, 753, 2429, 2491
Bsp143I GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
BspACI CCGC 7 cut(s) 778, 784, 1935, 2051, 2072, 2466, 2493
BspANI GGCC 5 cut(s) 636, 1011, 1747, 2107, 2222
BspDI ATCGAT 1 cut(s) 2255
BspLI GGNNCC 4 cut(s) 284, 1378, 1634, 2081
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 2 cut(s) 1521, 2500
BspT107I GGYRCC 1 cut(s) 2079
BspTNI GGTCTC 4 cut(s) 838, 1533, 1861, 2327
BsrDI GCAATG 2 cut(s) 794, 1241
BsrFI RCCGGY 2 cut(s) 636, 2294
BsrI ACTGG 4 cut(s) 696, 1380, 1949, 2147
BssAI RCCGGY 2 cut(s) 636, 2294
BssECI CCNNGG 4 cut(s) 108, 286, 1093, 1635
BssMI GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 5 cut(s) 242, 1544, 1637, 1757, 2084
Bst4CI ACNGT 6 cut(s) 365, 410, 455, 898, 1364, 1908
Bst6I CTCTTC 2 cut(s) 352, 1197
BstC8I GCNNGC 5 cut(s) 533, 1232, 1551, 1779, 2224
BstEII GGTNACC 1 cut(s) 236
BstH2I RGCGCY 1 cut(s) 2131
BstHHI GCGC 2 cut(s) 2130, 2444
BstKTI GATC 6 cut(s) 1516, 1606, 1672, 1897, 2255, 2508
BstMAI GTCTC 7 cut(s) 376, 673, 838, 904, 1533, 1861, 2327
BstMBI GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
BstMCI CGRYCG 1 cut(s) 2255
BstMWI GCNNNNNNNGC 4 cut(s) 257, 1127, 1236, 1405
BstNI CCWGG 5 cut(s) 242, 1544, 1637, 1757, 2084
BstNSI RCATGY 3 cut(s) 2122, 2425, 2433
BstPI GGTNACC 1 cut(s) 236
BstSCI CCNGG 8 cut(s) 107, 108, 240, 1542, 1635, 1755, 2082, 2508
BstSLI GKGCMC 2 cut(s) 103, 2429
BstV1I GCAGC 5 cut(s) 950, 972, 1108, 2065, 2169
BstXI CCANNNNNNTGG 2 cut(s) 856, 1506
Bsu15I ATCGAT 1 cut(s) 2255
BsuRI GGCC 5 cut(s) 636, 1011, 1747, 2107, 2222
BsuTUI ATCGAT 1 cut(s) 2255
BtsI GCAGTG 3 cut(s) 343, 1311, 1879
BtsIMutI CAGTG 4 cut(s) 343, 1311, 1387, 1879
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 5 cut(s) 533, 1232, 1551, 1779, 2224
CaiI CAGNNNCTG 1 cut(s) 902
CfoI GCGC 2 cut(s) 2130, 2444
Cfr10I RCCGGY 2 cut(s) 636, 2294
Cfr13I GGNCC 3 cut(s) 282, 1632, 1745
Cfr9I CCCGGG 1 cut(s) 108
ClaI ATCGAT 1 cut(s) 2255
CsiI ACCWGGT 1 cut(s) 1542
Csp6I GTAC 1 cut(s) 518
CspCI CAANNNNNGTGG 4 cut(s) 1090, 1125, 1300, 1335
CviQI GTAC 1 cut(s) 518
DpnI GATC 6 cut(s) 1515, 1605, 1671, 1896, 2254, 2507
DpnII GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
DriI GACNNNNNGTC 1 cut(s) 896
EaeI YGGCCR 2 cut(s) 1009, 2105
Eam1104I CTCTTC 2 cut(s) 352, 1197
Eam1105I GACNNNNNGTC 1 cut(s) 896
EarI CTCTTC 2 cut(s) 352, 1197
Eco130I CCWWGG 1 cut(s) 286
Eco147I AGGCCT 1 cut(s) 2222
Eco31I GGTCTC 4 cut(s) 838, 1533, 1861, 2327
Eco47I GGWCC 2 cut(s) 282, 1632
Eco47III AGCGCT 1 cut(s) 2129
Eco57I CTGAAG 2 cut(s) 1710, 1998
Eco88I CYCGRG 2 cut(s) 108, 173
Eco91I GGTNACC 1 cut(s) 236
EcoO65I GGTNACC 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 660
EcoRII CCWGG 5 cut(s) 240, 1542, 1635, 1755, 2082
EcoT14I CCWWGG 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 2124
ErhI CCWWGG 1 cut(s) 286
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 6 cut(s) 153, 268, 374, 1312, 1618, 1670
FauI CCCGC 1 cut(s) 2500
FauNDI CATATG 3 cut(s) 156, 444, 1912
FbaI TGATCA 2 cut(s) 1669, 1894
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 6 cut(s) 961, 964, 1122, 1935, 2054, 2183
Fsp4HI GCNGC 6 cut(s) 961, 964, 1122, 1935, 2054, 2183
FspBI CTAG 4 cut(s) 75, 1125, 2178, 2327
GlaI GCGC 2 cut(s) 2129, 2443
GluI GCNGC 6 cut(s) 961, 964, 1122, 1935, 2054, 2183
HaeII RGCGCY 1 cut(s) 2131
HaeIII GGCC 5 cut(s) 636, 1011, 1747, 2107, 2222
HapII CCGG 5 cut(s) 109, 637, 652, 2295, 2509
HhaI GCGC 2 cut(s) 2130, 2444
Hin6I GCGC 2 cut(s) 2128, 2442
HinP1I GCGC 2 cut(s) 2128, 2442
HincII GTYRAC 1 cut(s) 622
HindII GTYRAC 1 cut(s) 622
HpaI GTTAAC 1 cut(s) 622
HpaII CCGG 5 cut(s) 109, 637, 652, 2295, 2509
Hpy166II GTNNAC 6 cut(s) 17, 85, 622, 2155, 2300, 2427
Hpy8I GTNNAC 6 cut(s) 17, 85, 622, 2155, 2300, 2427
HpyAV CCTTC 7 cut(s) 72, 86, 700, 1529, 1736, 1845, 2073
HpyCH4III ACNGT 6 cut(s) 365, 410, 455, 898, 1364, 1908
HpyCH4IV ACGT 1 cut(s) 1994
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 1127, 1236, 1405
HpySE526I ACGT 1 cut(s) 1994
HspAI GCGC 2 cut(s) 2128, 2442
Ksp22I TGATCA 2 cut(s) 1669, 1894
KspAI GTTAAC 1 cut(s) 622
Kzo9I GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
LmnI GCTCC 4 cut(s) 748, 1186, 1376, 1987
Lsp1109I GCAGC 5 cut(s) 950, 972, 1108, 2065, 2169
LweI GCATC 7 cut(s) 80, 238, 437, 1339, 1964, 2131, 2266
MabI ACCWGGT 1 cut(s) 1542
MaeI CTAG 4 cut(s) 75, 1125, 2178, 2327
MaeII ACGT 1 cut(s) 1994
MaeIII GTNAC 6 cut(s) 236, 551, 760, 1364, 2088, 2417
MalI GATC 6 cut(s) 1515, 1605, 1671, 1896, 2254, 2507
MboI GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
MhlI GDGCHC 6 cut(s) 36, 103, 345, 753, 2429, 2491
MlsI TGGCCA 2 cut(s) 1011, 2107
MluNI TGGCCA 2 cut(s) 1011, 2107
MlyI GAGTC 5 cut(s) 172, 917, 1493, 2038, 2096
MmeI TCCRAC 3 cut(s) 223, 726, 2196
Mox20I TGGCCA 2 cut(s) 1011, 2107
Mph1103I ATGCAT 1 cut(s) 2124
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 2 cut(s) 1011, 2107
MseI TTAA 2 cut(s) 621, 701
MslI CAYNNNNRTG 6 cut(s) 854, 1347, 1449, 1504, 1596, 2231
Msp20I TGGCCA 2 cut(s) 1011, 2107
MspA1I CMGCKG 3 cut(s) 963, 1121, 2074
MspI CCGG 5 cut(s) 109, 637, 652, 2295, 2509
MspR9I CCNGG 8 cut(s) 109, 110, 242, 1544, 1637, 1757, 2084, 2510
Mva1269I GAATGC 2 cut(s) 786, 2023
MvaI CCWGG 5 cut(s) 242, 1544, 1637, 1757, 2084
MwoI GCNNNNNNNGC 4 cut(s) 257, 1127, 1236, 1405
NciI CCSGG 3 cut(s) 109, 110, 2510
NdeI CATATG 3 cut(s) 156, 444, 1912
NdeII GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
NlaIV GGNNCC 4 cut(s) 284, 1378, 1634, 2081
NmuCI GTSAC 4 cut(s) 236, 551, 2088, 2417
NsiI ATGCAT 1 cut(s) 2124
NspI RCATGY 3 cut(s) 2122, 2425, 2433
PaeR7I CTCGAG 1 cut(s) 173
PceI AGGCCT 1 cut(s) 2222
PciI ACATGT 2 cut(s) 2421, 2429
PctI GAATGC 2 cut(s) 786, 2023
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 8 cut(s) 38, 1163, 1172, 1207, 1760, 1766, 2284, 2396
PflFI GACNNNGTC 1 cut(s) 280
PflMI CCANNNNNTGG 3 cut(s) 1510, 1642, 1944
PkrI GCNGC 6 cut(s) 962, 965, 1123, 1936, 2055, 2184
Ple19I CGATCG 1 cut(s) 2255
PleI GAGTC 5 cut(s) 171, 917, 1492, 2037, 2095
PpsI GAGTC 5 cut(s) 171, 917, 1492, 2037, 2095
PscI ACATGT 2 cut(s) 2421, 2429
PsiI TTATAA 2 cut(s) 506, 2527
Psp6I CCWGG 5 cut(s) 240, 1542, 1635, 1755, 2082
PspEI GGTNACC 1 cut(s) 236
PspGI CCWGG 5 cut(s) 240, 1542, 1635, 1755, 2082
PspN4I GGNNCC 4 cut(s) 284, 1378, 1634, 2081
PspPI GGNCC 3 cut(s) 282, 1632, 1745
PstNI CAGNNNCTG 1 cut(s) 902
PsyI GACNNNGTC 1 cut(s) 280
PvuI CGATCG 1 cut(s) 2255
PvuII CAGCTG 2 cut(s) 963, 1121
RsaI GTAC 1 cut(s) 519
RsaNI GTAC 1 cut(s) 518
RseI CAYNNNNRTG 6 cut(s) 854, 1347, 1449, 1504, 1596, 2231
SaqAI TTAA 2 cut(s) 621, 701
SatI GCNGC 6 cut(s) 961, 964, 1122, 1935, 2054, 2183
Sau3AI GATC 6 cut(s) 1513, 1603, 1669, 1894, 2252, 2505
Sau96I GGNCC 3 cut(s) 282, 1632, 1745
SchI GAGTC 5 cut(s) 172, 917, 1493, 2038, 2096
ScrFI CCNGG 8 cut(s) 109, 110, 242, 1544, 1637, 1757, 2084, 2510
SduI GDGCHC 6 cut(s) 36, 103, 345, 753, 2429, 2491
SexAI ACCWGGT 1 cut(s) 1542
SfaNI GCATC 7 cut(s) 80, 238, 437, 1339, 1964, 2131, 2266
Sfr274I CTCGAG 1 cut(s) 173
SinI GGWCC 2 cut(s) 282, 1632
SlaI CTCGAG 1 cut(s) 173
SmaI CCCGGG 1 cut(s) 110
SmiMI CAYNNNNRTG 6 cut(s) 854, 1347, 1449, 1504, 1596, 2231
SmlI CTYRAG 4 cut(s) 143, 173, 1463, 2240
SmoI CTYRAG 4 cut(s) 143, 173, 1463, 2240
SseBI AGGCCT 1 cut(s) 2222
SsiI CCGC 7 cut(s) 778, 784, 1935, 2051, 2072, 2466, 2493
SspMI CTAG 4 cut(s) 75, 1125, 2178, 2327
StuI AGGCCT 1 cut(s) 2222
StyD4I CCNGG 8 cut(s) 107, 108, 240, 1542, 1635, 1755, 2082, 2508
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 6 cut(s) 365, 410, 455, 898, 1364, 1908
TaiI ACGT 1 cut(s) 1997
TaqI TCGA 5 cut(s) 36, 174, 802, 854, 2255
TaqII GACCGA 2 cut(s) 1107, 2277
TauI GCSGC 1 cut(s) 1937
TfiI GAWTC 8 cut(s) 38, 1163, 1172, 1207, 1760, 1766, 2284, 2396
Tru1I TTAA 2 cut(s) 621, 701
Tru9I TTAA 2 cut(s) 621, 701
TscAI CASTG 4 cut(s) 343, 1318, 1387, 1879
TseFI GTSAC 4 cut(s) 236, 551, 2088, 2417
TseI GCWGC 5 cut(s) 960, 963, 1121, 2053, 2182
Tsp45I GTSAC 4 cut(s) 236, 551, 2088, 2417
TspGWI ACGGA 1 cut(s) 1871
TspMI CCCGGG 1 cut(s) 108
TspRI CASTG 4 cut(s) 343, 1318, 1387, 1879
Tth111I GACNNNGTC 1 cut(s) 280
Van91I CCANNNNNTGG 3 cut(s) 1510, 1642, 1944
VneI GTGCAC 1 cut(s) 2425
VpaK11BI GGWCC 2 cut(s) 282, 1632
XapI RAATTY 4 cut(s) 387, 660, 1474, 2364
XbaI TCTAGA 1 cut(s) 74
XceI RCATGY 3 cut(s) 2122, 2425, 2433
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 173
XmaI CCCGGG 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 4 cut(s) 75, 1125, 2178, 2327
Zsp2I ATGCAT 1 cut(s) 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.