pycom11g12270

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0003793
Physical Location & Seq
Forward (+)
11192573 .. 11196811
4239 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g12270.5

Sequence Viewer

Length: 2583 bp
ATGGCTATTGCTGGTCTACACAATGCTTCTGTGCTTGAGCCTTCTTTCCTTAGAGACTCTCAATCTCATACATCTAGATGGATACGTGATGAAGGGAGGGTGAGTACCCGTGCATCCTCCCTACTACAAAGGTGGCGGGAGCTTGAGGATGAGCATGTGGTGAGTCGTGCCCAAGAGAGAGTTAGTGAGAGGTTGCTTGTACAAAGGAGTGATAGGTTGATCGATGATATCTCGAGGGAAGACACTACTGAAGACCACGGTAGTTACCACGCAGGTGATTTGGAGGATGTGAGTGTGGGTGAGAATGAGTGCGGAGTATGGTCCCGGGGTCAAATTGGGTCATCAAATGAACATGAGGAGTGCAGTAATATTAGCAGTGAGCATTCTGATTTTGGGGAGGTTGAAAGGGGGAGGGTGAGGCAAGTTTTTAGGGAATGGATGAATTGTGGTGTGGCTGAATATACGTCAAATGGTTCTCATATGAATAACAGTTCAAGGGCAGAGTGGCTCGGTGAAACTGAACAGGAGAGGGTGAGAATTGTAAGGGAGTGGGTACGAATTAACAGTCAGCAGAGAGGTGCTTCGGGTGACAATAGAGGAGAACAACCTGCTGAAATTGGTAATCAAATTGAAAGAGTTCGTGATGGATTGATTGTTAACCAAACTGAAGGCAGGTCTGAGCATACACGAAGGGGAATTCGTAAGTTATGTGGTAGACAGGCTATGCTGGATATGGTAAAGAAGGCTGAGAGGGAAAGACAAAGTGAACTTCAAGTCTTGTTGGAGCACCGGGCTGTATCACAGTTTGCTCACCGCAACCGCATTCAGTCATTGCTTAAAGGAAGATTCTTACGAAATGCTAGAGTGATTGAGAATGAGAGACCTACTTCCTTGGCTGAAAATGAATTAGGCTTATTGAGACAAAGGCACACAGTCTCTGGTCTTAGGGACGGTTTTTGCTCCAGATTGGATAGTTCTGTATCCGGTCAAGTAAGCAGCAGCCACTCTGAGACATCCTCTAGTGTTTCTAATGGTAGTAGAAATGGGGATGGTCAAGCAAACAACCCGCATGAGGTCCTAGCTGGGTTTTCTAAACAATCTGCTGCTAATGACGAGGCAAGTGAGGACCGAGAACGTGATAGCTGTGGAATATCAGATTTCAGGGATTTGGGTGGCAACACCATTCAAGATATAGATTCAGAAGAATCTAATGCTCCTGTAGAGGGTTGGCAACACCATTCAAGAAACTGGCAATGCTCAAGTATCACTGAATTTATCGAAAGGATGGATGTTACTGATTATAACATGATTGGGGACTTTCAAGCAACCACTTCTGTTGAGCAGCCCCAAGAAATTTTGCAAAATGATGCTGGTGGACACAGTAACATGCAAAAAGTCAGTCATATATCTAATGAGCAGTCTGAGCCAAGTGGGGAGGCAAGTGCAATAGGTGAACGGTCTGATCATATATATAATTTACTAGACAGTGTGCCTGGGGATGTAAATTTTCAAGAATTTACTTCCCACGTACAACGGTGGCAGGATCGAGTTTCAGAACAAGCTGAAAGGGACTGGCAACAACCTGTTGTTGCGTATAACGACTTGAGAGAAAATGTTGGGGAAGATACAACTGGGGATCAGCAGGAAACAACTGCTTATGAATGGTCCCAGGAGTTGCTGGAGAGCGAAGATAGAGAAAACAGTCATCTTGAAGAAGTTCCTGAAGTGTGGCACGAGGAAAGTGGTTTTCAGGAGGCTGTTCAAAGTTGGTTAGAAGAGCCATCGGACCAGGATGTTGATCCGGTCAGGCAGACTGACACATTTTATTTTCCGGATGATGATAATGCTCCTGGCACCGAGCTAAGGGAACTCCTAAGCAGGAGAAGGGTCTCTAATCTTCTTAGTAGTGGTTTCCGTCAGAATCTAGATCGACTGTTACAATCATATGTAGAAAGGCAAAGTCACGCTGCCATTGACTGGGAGCTGGATGATACATCACCTTCTCCTGAACCTGCAGAACAGGATCTAGAGCAGACTGGTGCATATCAAAATGGGGGTCAAGTGGATGCTGTTGAGAGTCATAGTCCTAGTACTGCTCTACGATCACAGCAAATACCTTCCTTGCCACTTTGGGACCAGGAGTCACACTCTGATCATTGGCCACAGCACGACATGCATCAACGTAACGGAATTGATTGGGAGATAATTAACGATATGAGGATTGACATGGCCAGGCTACACCAGAGGATGAATAACTTGCAAAGAATGATGGAGGCCTGCATGGATATGCAACTTGAGTTGCAGCGCTCGATAAGACAAGAAGTTTCTGATGCCCTGAATAGATCAGCTGGTTCACAAGGGGTGTGTGAAGACGGTCTGCTAGAAGATGGGTCTAAATGGGATCATGTAAGGAAAGGAATTTGCTGTATATGTTGTGAAAGCAGCATCGATTCTTTATTGTACAGATGTGGCCACATGTGCGCATGTTCAAAATGTGCTACTCAATTGGTTGAGAGTAGCGGAAAATGTCCGATGTGTAGAGCACCCGCGATTGAGGTGATTCGTGTTTATTCTGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

861

Amino Acids

97.56

Weight (kDa)

4.82

Isoelectric Point (pI)

59.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1302
AarI CACCTGC 1 cut(s) 263
Acc16I TGCGCA 1 cut(s) 2482
Acc36I ACCTGC 4 cut(s) 263, 616, 663, 2020
AccB1I GGYRCC 1 cut(s) 1853
AccB7I CCANNNNNTGG 1 cut(s) 1977
AccI GTMKAC 2 cut(s) 16, 715
AccII CGCG 1 cut(s) 2549
AccIII TCCGGA 1 cut(s) 1831
AciI CCGC 7 cut(s) 136, 312, 814, 820, 1067, 2520, 2547
AclWI GGATC 5 cut(s) 1551, 1644, 1793, 2031, 2409
AcoI YGGCCR 3 cut(s) 2159, 2229, 2470
AcsI RAATTY 6 cut(s) 696, 1271, 1353, 1504, 1514, 2418
AcuI CTGAAG 3 cut(s) 270, 687, 1743
AfaI GTAC 6 cut(s) 106, 201, 555, 1530, 2092, 2462
AfeI AGCGCT 1 cut(s) 2306
AfiI CCNNNNNNNGG 4 cut(s) 1072, 1223, 1863, 1977
AflIII ACRYGT 1 cut(s) 2475
AhdI GACNNNNNGTC 1 cut(s) 2140
AjnI CCWGG 6 cut(s) 1492, 1668, 1788, 1849, 2136, 2231
AleI CACNNNNGTG 1 cut(s) 273
AluBI AGCT 7 cut(s) 142, 1082, 1143, 1562, 1861, 1984, 2348
AluI AGCT 7 cut(s) 142, 1082, 1143, 1562, 1861, 1984, 2348
Alw21I GWGCWC 2 cut(s) 789, 2545
Alw26I GTCTC 6 cut(s) 48, 874, 913, 940, 1004, 1894
AlwI GGATC 5 cut(s) 1551, 1644, 1793, 2031, 2409
AlwNI CAGNNNCTG 1 cut(s) 938
Ama87I CYCGRG 2 cut(s) 232, 324
Aor13HI TCCGGA 1 cut(s) 1831
Aor51HI AGCGCT 1 cut(s) 2306
AoxI GGCC 4 cut(s) 2159, 2229, 2274, 2470
ApeKI GCWGC 7 cut(s) 996, 999, 1103, 1342, 1967, 2302, 2442
ApoI RAATTY 6 cut(s) 696, 1271, 1353, 1504, 1514, 2418
ArsI GACNNNNNNTTYG 6 cut(s) 550, 582, 1384, 1416, 2041, 2073
Asp700I GAANNNNTTC 2 cut(s) 636, 1716
AspLEI GCGC 2 cut(s) 2307, 2483
AspS9I GGNCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
AsuC2I CCSGG 3 cut(s) 325, 326, 791
AvaI CYCGRG 2 cut(s) 232, 324
AvaII GGWCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
BaeGI GKGCMC 1 cut(s) 172
BalI TGGCCA 3 cut(s) 2161, 2231, 2472
BanI GGYRCC 1 cut(s) 1853
BauI CACGAG 1 cut(s) 1733
BbsI GAAGAC 3 cut(s) 246, 258, 2376
Bbv12I GWGCWC 2 cut(s) 789, 2545
BbvI GCAGC 7 cut(s) 1008, 1011, 1090, 1354, 1954, 2314, 2454
BccI CCATC 7 cut(s) 72, 638, 1043, 1279, 1789, 2263, 2381
BciT130I CCWGG 6 cut(s) 1494, 1670, 1790, 1851, 2138, 2233
BciVI GTATCC 2 cut(s) 75, 991
BclI TGATCA 2 cut(s) 1462, 2152
BcnI CCSGG 3 cut(s) 325, 326, 791
BcoDI GTCTC 6 cut(s) 48, 874, 913, 940, 1004, 1894
BfaI CTAG 9 cut(s) 75, 861, 1020, 1079, 1481, 1925, 2027, 2088, 2381
BfmI CTRYAG 2 cut(s) 1218, 2013
BfoI RGCGCY 1 cut(s) 2308
BfuAI ACCTGC 4 cut(s) 263, 616, 663, 2020
BfuI GTATCC 2 cut(s) 75, 991
BisI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1968, 2303, 2443
BlsI GCNGC 7 cut(s) 998, 1001, 1105, 1344, 1969, 2304, 2444
BmcAI AGTACT 1 cut(s) 2092
Bme1390I CCNGG 9 cut(s) 325, 326, 791, 1494, 1670, 1790, 1851, 2138, 2233
Bme18I GGWCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
BmeRI GACNNNNNGTC 1 cut(s) 2140
BmeT110I CYCGRG 2 cut(s) 232, 324
BmgT120I GGNCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
BmiI GGNNCC 4 cut(s) 323, 1667, 1855, 2135
BmrFI CCNGG 9 cut(s) 325, 326, 791, 1494, 1670, 1790, 1851, 2138, 2233
BmrI ACTGGG 2 cut(s) 1641, 1987
BmsI GCATC 6 cut(s) 122, 1357, 2056, 2185, 2320, 2454
BmuI ACTGGG 2 cut(s) 1641, 1987
BpiI GAAGAC 3 cut(s) 246, 258, 2376
BplI GAGNNNNNCTC 4 cut(s) 1001, 1033, 2132, 2164
BpmI CTGGAG 2 cut(s) 946, 1700
Bpu10I CCTNAGC 2 cut(s) 1862, 1874
BpuEI CTTGAG 5 cut(s) 56, 164, 1243, 1624, 2315
BpuMI CCSGG 3 cut(s) 325, 326, 791
Bsa29I ATCGAT 2 cut(s) 222, 2448
BsaAI YACGTR 2 cut(s) 86, 1528
BsaBI GATNNNNATC 1 cut(s) 1797
BsaI GGTCTC 2 cut(s) 874, 1894
BsaJI CCNNGG 6 cut(s) 256, 324, 325, 891, 1493, 1668
BsaWI WCCGGW 3 cut(s) 983, 1801, 1831
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 4 cut(s) 1072, 1223, 1863, 1977
Bse1I ACTGG 5 cut(s) 1253, 1577, 1636, 1982, 2041
Bse3DI GCAATG 2 cut(s) 830, 1259
Bse8I GATNNNNATC 1 cut(s) 1797
BseAI TCCGGA 1 cut(s) 1831
BseBI CCWGG 6 cut(s) 1494, 1670, 1790, 1851, 2138, 2233
BseCI ATCGAT 2 cut(s) 222, 2448
BseDI CCNNGG 6 cut(s) 256, 324, 325, 891, 1493, 1668
BseJI GATNNNNATC 1 cut(s) 1797
BseLI CCNNNNNNNGG 4 cut(s) 1072, 1223, 1863, 1977
BseMI GCAATG 2 cut(s) 830, 1259
BseMII CTCAG 4 cut(s) 669, 738, 999, 1413
BseNI ACTGG 5 cut(s) 1253, 1577, 1636, 1982, 2041
BseRI GAGGAG 2 cut(s) 371, 612
BseSI GKGCMC 1 cut(s) 172
BseXI GCAGC 7 cut(s) 1008, 1011, 1090, 1354, 1954, 2314, 2454
BseYI CCCAGC 1 cut(s) 1082
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 2549
BshFI GGCC 4 cut(s) 2161, 2231, 2276, 2472
BshNI GGYRCC 1 cut(s) 1853
BshVI ATCGAT 2 cut(s) 222, 2448
BsiHKAI GWGCWC 2 cut(s) 789, 2545
BsiHKCI CYCGRG 2 cut(s) 232, 324
BsiSI CCGG 5 cut(s) 325, 790, 984, 1802, 1832
BslFI GGGAC 6 cut(s) 307, 962, 1328, 1583, 1651, 2147
BslI CCNNNNNNNGG 4 cut(s) 1072, 1223, 1863, 1977
BsmAI GTCTC 6 cut(s) 48, 874, 913, 940, 1004, 1894
BsmFI GGGAC 6 cut(s) 307, 962, 1328, 1583, 1651, 2147
BsmI GAATGC 2 cut(s) 382, 822
BsnI GGCC 4 cut(s) 2161, 2231, 2276, 2472
Bso31I GGTCTC 2 cut(s) 874, 1894
BsoBI CYCGRG 2 cut(s) 232, 324
Bsp1286I GDGCHC 3 cut(s) 172, 789, 2545
Bsp13I TCCGGA 1 cut(s) 1831
Bsp1407I TGTACA 2 cut(s) 199, 2460
BspACI CCGC 7 cut(s) 136, 312, 814, 820, 1067, 2520, 2547
BspANI GGCC 4 cut(s) 2161, 2231, 2276, 2472
BspCNI CTCAG 4 cut(s) 670, 739, 1000, 1414
BspDI ATCGAT 2 cut(s) 222, 2448
BspEI TCCGGA 1 cut(s) 1831
BspFNI CGCG 1 cut(s) 2549
BspLI GGNNCC 4 cut(s) 323, 1667, 1855, 2135
BspMAI CTGCAG 1 cut(s) 2017
BspMI ACCTGC 4 cut(s) 263, 616, 663, 2020
BspPI GGATC 5 cut(s) 1551, 1644, 1793, 2031, 2409
BspQI GCTCTTC 1 cut(s) 1770
BspT107I GGYRCC 1 cut(s) 1853
BspTNI GGTCTC 2 cut(s) 874, 1894
BsrDI GCAATG 2 cut(s) 830, 1259
BsrGI TGTACA 2 cut(s) 199, 2460
BsrI ACTGG 5 cut(s) 1253, 1577, 1636, 1982, 2041
BssECI CCNNGG 6 cut(s) 256, 324, 325, 891, 1493, 1668
BssSI CACGAG 1 cut(s) 1733
BssT1I CCWWGG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 1733
Bst2UI CCWGG 6 cut(s) 1494, 1670, 1790, 1851, 2138, 2233
Bst6I CTCTTC 1 cut(s) 1770
BstAPI GCANNNNNTGC 1 cut(s) 2173
BstAUI TGTACA 2 cut(s) 199, 2460
BstBAI YACGTR 2 cut(s) 86, 1528
BstC8I GCNNGC 1 cut(s) 2278
BstDEI CTNAG 9 cut(s) 50, 678, 747, 944, 1008, 1422, 1862, 1874, 1901
BstDSI CCRYGG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 2549
BstH2I RGCGCY 1 cut(s) 2308
BstHHI GCGC 2 cut(s) 2307, 2483
BstMAI GTCTC 6 cut(s) 48, 874, 913, 940, 1004, 1894
BstMWI GCNNNNNNNGC 3 cut(s) 1423, 2173, 2478
BstNI CCWGG 6 cut(s) 1494, 1670, 1790, 1851, 2138, 2233
BstNSI RCATGY 5 cut(s) 158, 1390, 2176, 2479, 2487
BstSCI CCNGG 9 cut(s) 323, 324, 789, 1492, 1668, 1788, 1849, 2136, 2231
BstSFI CTRYAG 2 cut(s) 1218, 2013
BstSLI GKGCMC 1 cut(s) 172
BstUI CGCG 1 cut(s) 2549
BstV1I GCAGC 7 cut(s) 1008, 1011, 1090, 1354, 1954, 2314, 2454
BstV2I GAAGAC 3 cut(s) 246, 258, 2376
BstX2I RGATCY 1 cut(s) 2023
BstYI RGATCY 1 cut(s) 2023
Bsu15I ATCGAT 2 cut(s) 222, 2448
BsuI GTATCC 2 cut(s) 75, 991
BsuRI GGCC 4 cut(s) 2161, 2231, 2276, 2472
BsuTUI ATCGAT 2 cut(s) 222, 2448
BtgI CCRYGG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 3 cut(s) 382, 1266, 1492
BveI ACCTGC 4 cut(s) 263, 616, 663, 2020
Cac8I GCNNGC 1 cut(s) 2278
CaiI CAGNNNCTG 1 cut(s) 938
CfoI GCGC 2 cut(s) 2307, 2483
Cfr13I GGNCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
Cfr9I CCCGGG 1 cut(s) 324
ClaI ATCGAT 2 cut(s) 222, 2448
Csp6I GTAC 6 cut(s) 105, 200, 554, 1529, 2091, 2461
CspCI CAANNNNNGTGG 2 cut(s) 1318, 1353
CviQI GTAC 6 cut(s) 105, 200, 554, 1529, 2091, 2461
DdeI CTNAG 9 cut(s) 50, 678, 747, 944, 1008, 1422, 1862, 1874, 1901
DriI GACNNNNNGTC 1 cut(s) 2140
EaeI YGGCCR 3 cut(s) 2159, 2229, 2470
Eam1104I CTCTTC 1 cut(s) 1770
Eam1105I GACNNNNNGTC 1 cut(s) 2140
EarI CTCTTC 1 cut(s) 1770
Eco130I CCWWGG 1 cut(s) 891
Eco147I AGGCCT 1 cut(s) 2276
Eco31I GGTCTC 2 cut(s) 874, 1894
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
Eco47III AGCGCT 1 cut(s) 2306
Eco57I CTGAAG 3 cut(s) 270, 687, 1743
Eco88I CYCGRG 2 cut(s) 232, 324
EcoO109I RGGNCCY 1 cut(s) 1075
EcoRI GAATTC 1 cut(s) 696
EcoRII CCWGG 6 cut(s) 1492, 1668, 1788, 1849, 2136, 2231
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 2178
ErhI CCWWGG 1 cut(s) 891
FaqI GGGAC 6 cut(s) 307, 962, 1328, 1583, 1651, 2147
FauI CCCGC 3 cut(s) 129, 1074, 2554
FauNDI CATATG 2 cut(s) 480, 1945
FbaI TGATCA 2 cut(s) 1462, 2152
FblI GTMKAC 2 cut(s) 16, 715
Fnu4HI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1968, 2303, 2443
Fsp4HI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1968, 2303, 2443
FspAI RTGCGCAY 1 cut(s) 2482
FspBI CTAG 9 cut(s) 75, 861, 1020, 1079, 1481, 1925, 2027, 2088, 2381
FspI TGCGCA 1 cut(s) 2482
GlaI GCGC 2 cut(s) 2306, 2482
GluI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1968, 2303, 2443
GsaI CCCAGC 1 cut(s) 1086
GsuI CTGGAG 2 cut(s) 946, 1700
HaeII RGCGCY 1 cut(s) 2308
HaeIII GGCC 4 cut(s) 2161, 2231, 2276, 2472
HapII CCGG 5 cut(s) 325, 790, 984, 1802, 1832
HhaI GCGC 2 cut(s) 2307, 2483
Hin6I GCGC 2 cut(s) 2305, 2481
HinP1I GCGC 2 cut(s) 2305, 2481
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 5 cut(s) 325, 790, 984, 1802, 1832
Hpy166II GTNNAC 7 cut(s) 17, 658, 716, 767, 1376, 1454, 2354
Hpy8I GTNNAC 7 cut(s) 17, 658, 716, 767, 1376, 1454, 2354
HpyAV CCTTC 8 cut(s) 51, 86, 662, 684, 736, 1878, 2010, 2127
HpyCH4IV ACGT 5 cut(s) 85, 464, 1135, 1527, 2182
HpyF10VI GCNNNNNNNGC 3 cut(s) 1423, 2173, 2478
HpyF3I CTNAG 9 cut(s) 50, 678, 747, 944, 1008, 1422, 1862, 1874, 1901
HpySE526I ACGT 5 cut(s) 85, 464, 1135, 1527, 2182
HspAI GCGC 2 cut(s) 2305, 2481
Kpn2I TCCGGA 1 cut(s) 1831
Ksp22I TGATCA 2 cut(s) 1462, 2152
KspAI GTTAAC 1 cut(s) 658
LguI GCTCTTC 1 cut(s) 1770
LmnI GCTCC 6 cut(s) 139, 784, 965, 1219, 1852, 1981
Lsp1109I GCAGC 7 cut(s) 1008, 1011, 1090, 1354, 1954, 2314, 2454
LweI GCATC 6 cut(s) 122, 1357, 2056, 2185, 2320, 2454
MaeI CTAG 9 cut(s) 75, 861, 1020, 1079, 1481, 1925, 2027, 2088, 2381
MaeII ACGT 5 cut(s) 85, 464, 1135, 1527, 2182
MaeIII GTNAC 8 cut(s) 263, 587, 1291, 1382, 1935, 1961, 2142, 2183
MfeI CAATTG 1 cut(s) 2504
MflI RGATCY 1 cut(s) 2023
MhlI GDGCHC 3 cut(s) 172, 789, 2545
MlsI TGGCCA 3 cut(s) 2161, 2231, 2472
MluNI TGGCCA 3 cut(s) 2161, 2231, 2472
MlyI GAGTC 4 cut(s) 50, 172, 2086, 2150
MmeI TCCRAC 1 cut(s) 762
Mox20I TGGCCA 3 cut(s) 2161, 2231, 2472
Mph1103I ATGCAT 1 cut(s) 2178
MroI TCCGGA 1 cut(s) 1831
MroXI GAANNNNTTC 2 cut(s) 636, 1716
MscI TGGCCA 3 cut(s) 2161, 2231, 2472
MseI TTAA 4 cut(s) 561, 657, 837, 2208
MslI CAYNNNNRTG 3 cut(s) 76, 273, 2285
Msp20I TGGCCA 3 cut(s) 2161, 2231, 2472
MspA1I CMGCKG 1 cut(s) 2348
MspI CCGG 5 cut(s) 325, 790, 984, 1802, 1832
MspR9I CCNGG 9 cut(s) 325, 326, 791, 1494, 1670, 1790, 1851, 2138, 2233
MunI CAATTG 1 cut(s) 2504
Mva1269I GAATGC 2 cut(s) 382, 822
MvaI CCWGG 6 cut(s) 1494, 1670, 1790, 1851, 2138, 2233
MvnI CGCG 1 cut(s) 2549
MwoI GCNNNNNNNGC 3 cut(s) 1423, 2173, 2478
NciI CCSGG 3 cut(s) 325, 326, 791
NdeI CATATG 2 cut(s) 480, 1945
NlaIV GGNNCC 4 cut(s) 323, 1667, 1855, 2135
NmuCI GTSAC 3 cut(s) 587, 1961, 2142
NsbI TGCGCA 1 cut(s) 2482
NsiI ATGCAT 1 cut(s) 2178
NspI RCATGY 5 cut(s) 158, 1390, 2176, 2479, 2487
OliI CACNNNNGTG 1 cut(s) 273
PaeR7I CTCGAG 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 263
PceI AGGCCT 1 cut(s) 2276
PciI ACATGT 1 cut(s) 2475
PciSI GCTCTTC 1 cut(s) 1770
PctI GAATGC 2 cut(s) 382, 822
PdmI GAANNNNTTC 2 cut(s) 636, 1716
PfeI GAWTC 6 cut(s) 846, 1196, 1205, 1921, 2450, 2560
PflMI CCANNNNNTGG 1 cut(s) 1977
PkrI GCNGC 7 cut(s) 998, 1001, 1105, 1344, 1969, 2304, 2444
PleI GAGTC 4 cut(s) 50, 171, 2085, 2149
PpsI GAGTC 4 cut(s) 50, 171, 2085, 2149
Ppu21I YACGTR 2 cut(s) 86, 1528
PpuMI RGGWCCY 1 cut(s) 1075
PscI ACATGT 1 cut(s) 2475
PsiI TTATAA 1 cut(s) 1302
Psp5II RGGWCCY 1 cut(s) 1075
Psp6I CCWGG 6 cut(s) 1492, 1668, 1788, 1849, 2136, 2231
PspFI CCCAGC 1 cut(s) 1082
PspGI CCWGG 6 cut(s) 1492, 1668, 1788, 1849, 2136, 2231
PspN4I GGNNCC 4 cut(s) 323, 1667, 1855, 2135
PspPI GGNCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
PspPPI RGGWCCY 1 cut(s) 1075
PstI CTGCAG 1 cut(s) 2017
PstNI CAGNNNCTG 1 cut(s) 938
PsuI RGATCY 1 cut(s) 2023
PvuII CAGCTG 1 cut(s) 2348
RsaI GTAC 6 cut(s) 106, 201, 555, 1530, 2092, 2462
RsaNI GTAC 6 cut(s) 105, 200, 554, 1529, 2091, 2461
RseI CAYNNNNRTG 3 cut(s) 76, 273, 2285
SapI GCTCTTC 1 cut(s) 1770
SaqAI TTAA 4 cut(s) 561, 657, 837, 2208
SatI GCNGC 7 cut(s) 997, 1000, 1104, 1343, 1968, 2303, 2443
Sau96I GGNCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
ScaI AGTACT 1 cut(s) 2092
SchI GAGTC 4 cut(s) 50, 172, 2086, 2150
ScrFI CCNGG 9 cut(s) 325, 326, 791, 1494, 1670, 1790, 1851, 2138, 2233
SduI GDGCHC 3 cut(s) 172, 789, 2545
SfaNI GCATC 6 cut(s) 122, 1357, 2056, 2185, 2320, 2454
SfcI CTRYAG 2 cut(s) 1218, 2013
Sfr274I CTCGAG 1 cut(s) 232
SinI GGWCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
SlaI CTCGAG 1 cut(s) 232
SmaI CCCGGG 1 cut(s) 326
SmiMI CAYNNNNRTG 3 cut(s) 76, 273, 2285
SmlI CTYRAG 6 cut(s) 35, 143, 232, 1258, 1603, 2294
SmoI CTYRAG 6 cut(s) 35, 143, 232, 1258, 1603, 2294
SseBI AGGCCT 1 cut(s) 2276
SsiI CCGC 7 cut(s) 136, 312, 814, 820, 1067, 2520, 2547
SspI AATATT 1 cut(s) 370
SspMI CTAG 9 cut(s) 75, 861, 1020, 1079, 1481, 1925, 2027, 2088, 2381
StuI AGGCCT 1 cut(s) 2276
StyD4I CCNGG 9 cut(s) 323, 324, 789, 1492, 1668, 1788, 1849, 2136, 2231
StyI CCWWGG 1 cut(s) 891
TaiI ACGT 5 cut(s) 88, 467, 1138, 1530, 2185
TaqI TCGA 7 cut(s) 222, 233, 1278, 1546, 1930, 2309, 2448
TaqII GACCGA 1 cut(s) 1143
TatI WGTACW 3 cut(s) 199, 2090, 2460
TfiI GAWTC 6 cut(s) 846, 1196, 1205, 1921, 2450, 2560
Tru1I TTAA 4 cut(s) 561, 657, 837, 2208
Tru9I TTAA 4 cut(s) 561, 657, 837, 2208
TscAI CASTG 3 cut(s) 382, 1273, 1492
TseFI GTSAC 3 cut(s) 587, 1961, 2142
TseI GCWGC 7 cut(s) 996, 999, 1103, 1342, 1967, 2302, 2442
Tsp45I GTSAC 3 cut(s) 587, 1961, 2142
TspDTI ATGAA 7 cut(s) 105, 363, 455, 497, 918, 1674, 2264
TspGWI ACGGA 2 cut(s) 1904, 2202
TspMI CCCGGG 1 cut(s) 324
TspRI CASTG 3 cut(s) 382, 1273, 1492
Van91I CCANNNNNTGG 1 cut(s) 1977
VpaK11BI GGWCC 6 cut(s) 321, 1075, 1126, 1665, 1786, 2134
XapI RAATTY 6 cut(s) 696, 1271, 1353, 1504, 1514, 2418
XbaI TCTAGA 3 cut(s) 74, 1924, 2026
XceI RCATGY 5 cut(s) 158, 1390, 2176, 2479, 2487
XhoI CTCGAG 1 cut(s) 232
XmaI CCCGGG 1 cut(s) 324
XmiI GTMKAC 2 cut(s) 16, 715
XmnI GAANNNNTTC 2 cut(s) 636, 1716
XspI CTAG 9 cut(s) 75, 861, 1020, 1079, 1481, 1925, 2027, 2088, 2381
ZrmI AGTACT 1 cut(s) 2092
Zsp2I ATGCAT 1 cut(s) 2178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.