Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003128

Physical Location & Seq

Genomic Coordinates Forward (+)

12970597 .. 12973075

Gene Structure 2479 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1131500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGCGTCGTCGGCGAGAGTAGATCTGGACGGCAACCCGATAAAGCCGATTACGATATGCATGATTGGAGCCGGAGGGTTCATTGGGTCCCACCTCTGCGAGAAGCTGATGTCGGAGACGCCGCACAAGGTCCTAGCTCTGGACGTCTACAATGACAAGATCAAGCATCTGCTCGAGCCCGAGAACGCGCACCCCTGGTCCGATCGCATCCAGTTCCACCGACTCAACATCAAGCAGGACTCGCGCCTCGAAGGGCTCATCAAAATGGCAGATCTGACGATTAACCTGGCGGCGATCTGTACTCCGGCGGACTACAACACCCGCCCGCTTGACACCATCTACAGCAACTTCATCGATGCTTTGCCGGTGGTGAAGTACTGTTCCGAAAACAACAAGCGGCTGATTCACTTTTCTACTTGTGAAGTGTATGGGAAAACCATTGGAAGCTATCTTCCTAAAGATAGCCCTCTTCGTCAGGATCCTGCTTATTATGTGCTTAAAGAAGATGAATCCCCTTGCATTTTTGGATCTATTGAGAAGCAGAGATGGTCCTATGCATGTGCAAAGCAATTGATTGAGAGGCTGATCTATGCCGAGGGTGCGGAAAATGGTCTTGAATTTACCATTGTGAGACCCTTCAACTGGATTGGACCTAGGATGGATTTCATTCCTGGCATTGACGGCCCAAGTGAGGGTGTTCCAAGAGTTCTTGCATGCTTCAGTAATAATCTTCTTCGCCGTGAGCCTCTCAAGCTCGTTGATGGCGGCCAATCTCAGAGAACTTTTGTCTACATAAAGGATGCTATTGAAGCTGTTATGTTGATGATCGAAAATCCTGCTAGGGCCAATGGTCACATCTTCAATGTAGGCAACCCTAACAATGAAGTTACTGTTAGGCAACTTGGTGAAATGATGACTGAGGTTTATGCGAAAGTAAGTAGCGAACCAAAATTGGAGACACCCACCATTGATGTTAGCTCTCAAGAATTCTATGGAGTGGGATATGATGATAGTGACAAGAGAATTCCTGACATGACTATTATCAACAAGCAGCTTGGTACGTATCTTTCATTTACCTCGTCTTTAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009987 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019438 GO:0034641 GO:0034654 GO:0036094 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048037 GO:0048040 GO:0048046 GO:0050662 GO:0051287 GO:0055086 GO:0071704 GO:0097159 GO:1901135 GO:1901137 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Epimerase

Protein Analysis

364

Amino Acids

40.76

Weight (kDa)

5.49

Isoelectric Point (pI)

48.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	19 - 290	7.6e-48	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	20 - 139	2.1e-06	Polysaccharide biosynthesis protein
3Beta_HSD	PF01073	21 - 145	4.7e-06	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	23 - 353	6.4e-23	GDP-mannose 4,6 dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08200
fragaria_vesca	FvH4_3g30060 FvH4_3g30060 FvH4_3g30060 FvH4_3g30060
malus_domestica	MD03G1131500.v1.1 MD03G1131900.v1.1 MD03G1161900.v1.1 MD11G1154500.v1.1 MD11G1215900.v1.1 MD16G1216600.v1.1 MD16G1216800.v1.1
prunus_persica	Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1
pyrus_communis	pycom11g12600 pycom11g12610
rosa_chinensis	RchiOBHm_Chr5g0055821
rosa_laevigata	RLG00000002609 RLG00000035011
rosa_multiflora	Rmu_sc0003306.1_g000005 Rmu_sc0003500.1_g000032 Rmu_sc0003500.1_g000033 Rmu_sc0007802.1_g000009
rosa_roxburghii	Rroxscaffold_1G00024520 Rroxscaffold_1G00052150
rosa_rugosa	Rorug05G0295000
rosa_samantha	Rh5AG192800 Rh5AG365900 Rh5BG190400 Rh5BG376600 Rh5CG399600 Rh5DG192100 Rh5DG389500 Rh5DG389600 Rh7BG106200
rosa_wichuraiana	Rw5G034330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	147
AccI	GTMKAC	2 cut(s)	147, 789
AccII	CGCG	2 cut(s)	188, 244
AciI	CCGC	8 cut(s)	122, 290, 308, 322, 326, 397, 602, 765
AclWI	GGATC	3 cut(s)	473, 486, 535
AcoI	YGGCCR	1 cut(s)	766
AcsI	RAATTY	3 cut(s)	617, 986, 1023
AcuI	CTGAAG	1 cut(s)	703
AcyI	GRCGYC	3 cut(s)	5, 119, 144
AfaI	GTAC	3 cut(s)	301, 377, 1060
AfiI	CCNNNNNNNGG	4 cut(s)	139, 642, 691, 692
AgsI	TTSAA	4 cut(s)	617, 640, 809, 862
AjnI	CCWGG	3 cut(s)	194, 285, 670
AluBI	AGCT	7 cut(s)	106, 137, 447, 754, 812, 978, 1054
AluI	AGCT	7 cut(s)	106, 137, 447, 754, 812, 978, 1054
Alw26I	GTCTC	3 cut(s)	110, 625, 950
AlwI	GGATC	3 cut(s)	473, 486, 535
Ama87I	CYCGRG	2 cut(s)	173, 179
AoxI	GGCC	3 cut(s)	682, 766, 843
ApeKI	GCWGC	1 cut(s)	1051
ApoI	RAATTY	3 cut(s)	617, 986, 1023
AspA2I	CCTAGG	1 cut(s)	653
AspLEI	GCGC	2 cut(s)	190, 246
AspS9I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
AsuHPI	GGTGA	2 cut(s)	382, 917
AvaI	CYCGRG	2 cut(s)	173, 179
AvaII	GGWCC	5 cut(s)	87, 130, 198, 549, 650
AvrII	CCTAGG	1 cut(s)	653
BamHI	GGATCC	1 cut(s)	478
BanII	GRGCYC	2 cut(s)	180, 258
BbvI	GCAGC	1 cut(s)	1063
BccI	CCATC	4 cut(s)	344, 540, 652, 755
BceAI	ACGGC	3 cut(s)	46, 697, 723
BcgI	CGANNNNNNTGC	4 cut(s)	334, 368, 818, 852
BciT130I	CCWGG	3 cut(s)	196, 287, 672
BcoDI	GTCTC	3 cut(s)	110, 625, 950
BfaI	CTAG	3 cut(s)	134, 654, 840
BfmI	CTRYAG	1 cut(s)	340
BglII	AGATCT	2 cut(s)	22, 271
BisI	GCNGC	5 cut(s)	122, 291, 398, 766, 1052
BlnI	CCTAGG	1 cut(s)	653
BlsI	GCNGC	5 cut(s)	123, 292, 399, 767, 1053
BmcAI	AGTACT	1 cut(s)	377
Bme1390I	CCNGG	3 cut(s)	196, 287, 672
Bme18I	GGWCC	5 cut(s)	87, 130, 198, 549, 650
BmeT110I	CYCGRG	2 cut(s)	173, 179
BmgT120I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
BmiI	GGNNCC	4 cut(s)	70, 88, 89, 480
BmrFI	CCNGG	3 cut(s)	196, 287, 672
BmsI	GCATC	4 cut(s)	175, 216, 346, 790
BpuEI	CTTGAG	2 cut(s)	734, 966
Bsa29I	ATCGAT	1 cut(s)	354
BsaAI	YACGTR	1 cut(s)	1062
BsaHI	GRCGYC	3 cut(s)	5, 119, 144
BsaI	GGTCTC	1 cut(s)	625
BsaJI	CCNNGG	3 cut(s)	194, 594, 653
Bsc4I	CCNNNNNNNGG	4 cut(s)	139, 642, 691, 692
Bse118I	RCCGGY	1 cut(s)	364
Bse1I	ACTGG	2 cut(s)	211, 647
BseBI	CCWGG	3 cut(s)	196, 287, 672
BseCI	ATCGAT	1 cut(s)	354
BseDI	CCNNGG	3 cut(s)	194, 594, 653
BseGI	GGATG	3 cut(s)	207, 663, 805
BseLI	CCNNNNNNNGG	4 cut(s)	139, 642, 691, 692
BseMII	CTCAG	2 cut(s)	788, 909
BseNI	ACTGG	2 cut(s)	211, 647
BseXI	GCAGC	1 cut(s)	1063
Bsh1236I	CGCG	2 cut(s)	188, 244
Bsh1285I	CGRYCG	1 cut(s)	205
BshFI	GGCC	3 cut(s)	684, 768, 845
BshVI	ATCGAT	1 cut(s)	354
BsiEI	CGRYCG	1 cut(s)	205
BsiHKCI	CYCGRG	2 cut(s)	173, 179
BsiSI	CCGG	3 cut(s)	72, 305, 365
BslFI	GGGAC	1 cut(s)	73
BslI	CCNNNNNNNGG	4 cut(s)	139, 642, 691, 692
BsmAI	GTCTC	3 cut(s)	110, 625, 950
BsmBI	CGTCTC	1 cut(s)	110
BsmFI	GGGAC	1 cut(s)	73
BsnI	GGCC	3 cut(s)	684, 768, 845
Bso31I	GGTCTC	1 cut(s)	625
BsoBI	CYCGRG	2 cut(s)	173, 179
Bsp1286I	GDGCHC	2 cut(s)	180, 258
Bsp143I	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
BspACI	CCGC	8 cut(s)	122, 290, 308, 322, 326, 397, 602, 765
BspANI	GGCC	3 cut(s)	684, 768, 845
BspCNI	CTCAG	2 cut(s)	787, 910
BspDI	ATCGAT	1 cut(s)	354
BspFNI	CGCG	2 cut(s)	188, 244
BspLI	GGNNCC	4 cut(s)	70, 88, 89, 480
BspPI	GGATC	3 cut(s)	473, 486, 535
BspTNI	GGTCTC	1 cut(s)	625
BsrFI	RCCGGY	1 cut(s)	364
BsrI	ACTGG	2 cut(s)	211, 647
BssAI	RCCGGY	1 cut(s)	364
BssECI	CCNNGG	3 cut(s)	194, 594, 653
BssMI	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
BssNI	GRCGYC	3 cut(s)	5, 119, 144
BssT1I	CCWWGG	1 cut(s)	653
Bst2UI	CCWGG	3 cut(s)	196, 287, 672
Bst4CI	ACNGT	2 cut(s)	380, 892
Bst6I	CTCTTC	1 cut(s)	474
BstACI	GRCGYC	3 cut(s)	5, 119, 144
BstBAI	YACGTR	1 cut(s)	1062
BstC8I	GCNNGC	2 cut(s)	326, 715
BstDEI	CTNAG	2 cut(s)	774, 918
BstF5I	GGATG	3 cut(s)	207, 663, 805
BstFNI	CGCG	2 cut(s)	188, 244
BstHHI	GCGC	2 cut(s)	190, 246
BstKTI	GATC	9 cut(s)	25, 162, 205, 274, 297, 481, 530, 588, 828
BstMAI	GTCTC	3 cut(s)	110, 625, 950
BstMBI	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
BstMCI	CGRYCG	1 cut(s)	205
BstMWI	GCNNNNNNNGC	6 cut(s)	11, 241, 599, 681, 751, 809
BstNI	CCWGG	3 cut(s)	196, 287, 672
BstNSI	RCATGY	2 cut(s)	561, 717
BstSCI	CCNGG	3 cut(s)	194, 285, 670
BstSFI	CTRYAG	1 cut(s)	340
BstSNI	TACGTA	1 cut(s)	1062
BstUI	CGCG	2 cut(s)	188, 244
BstV1I	GCAGC	1 cut(s)	1063
BstX2I	RGATCY	4 cut(s)	22, 271, 478, 527
BstYI	RGATCY	4 cut(s)	22, 271, 478, 527
Bsu15I	ATCGAT	1 cut(s)	354
BsuRI	GGCC	3 cut(s)	684, 768, 845
BsuTUI	ATCGAT	1 cut(s)	354
BtsCI	GGATG	3 cut(s)	207, 663, 805
Cac8I	GCNNGC	2 cut(s)	326, 715
CfoI	GCGC	2 cut(s)	190, 246
Cfr10I	RCCGGY	1 cut(s)	364
Cfr13I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
ClaI	ATCGAT	1 cut(s)	354
CseI	GACGC	1 cut(s)	127
Csp6I	GTAC	3 cut(s)	300, 376, 1059
CviAII	CATG	4 cut(s)	61, 558, 714, 1033
CviQI	GTAC	3 cut(s)	300, 376, 1059
DdeI	CTNAG	2 cut(s)	774, 918
DpnI	GATC	9 cut(s)	24, 161, 204, 273, 296, 480, 529, 587, 827
DpnII	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
EaeI	YGGCCR	1 cut(s)	766
Eam1104I	CTCTTC	1 cut(s)	474
EarI	CTCTTC	1 cut(s)	474
EciI	GGCGGA	1 cut(s)	323
Eco105I	TACGTA	1 cut(s)	1062
Eco130I	CCWWGG	1 cut(s)	653
Eco24I	GRGCYC	2 cut(s)	180, 258
Eco31I	GGTCTC	1 cut(s)	625
Eco47I	GGWCC	5 cut(s)	87, 130, 198, 549, 650
Eco57I	CTGAAG	1 cut(s)	703
Eco88I	CYCGRG	2 cut(s)	173, 179
EcoO109I	RGGNCCY	2 cut(s)	87, 130
EcoRI	GAATTC	2 cut(s)	986, 1023
EcoRII	CCWGG	3 cut(s)	194, 285, 670
EcoT14I	CCWWGG	1 cut(s)	653
EcoT22I	ATGCAT	2 cut(s)	62, 559
EcoT38I	GRGCYC	2 cut(s)	180, 258
ErhI	CCWWGG	1 cut(s)	653
Esp3I	CGTCTC	1 cut(s)	110
FaeI	CATG	4 cut(s)	64, 561, 717, 1036
FaqI	GGGAC	1 cut(s)	73
FatI	CATG	4 cut(s)	60, 557, 713, 1032
FauI	CCCGC	2 cut(s)	329, 333
FblI	GTMKAC	2 cut(s)	147, 789
Fnu4HI	GCNGC	5 cut(s)	122, 291, 398, 766, 1052
FokI	GGATG	3 cut(s)	194, 670, 812
FriOI	GRGCYC	2 cut(s)	180, 258
Fsp4HI	GCNGC	5 cut(s)	122, 291, 398, 766, 1052
FspBI	CTAG	3 cut(s)	134, 654, 840
GlaI	GCGC	2 cut(s)	189, 245
GluI	GCNGC	5 cut(s)	122, 291, 398, 766, 1052
HaeIII	GGCC	3 cut(s)	684, 768, 845
HapII	CCGG	3 cut(s)	72, 305, 365
HgaI	GACGC	1 cut(s)	127
HhaI	GCGC	2 cut(s)	190, 246
Hin1I	GRCGYC	3 cut(s)	5, 119, 144
Hin1II	CATG	4 cut(s)	64, 561, 717, 1036
Hin6I	GCGC	2 cut(s)	188, 244
HinP1I	GCGC	2 cut(s)	188, 244
HinfI	GANTC	4 cut(s)	222, 239, 403, 509
HpaII	CCGG	3 cut(s)	72, 305, 365
HphI	GGTGA	2 cut(s)	382, 917
Hpy166II	GTNNAC	2 cut(s)	148, 790
Hpy188I	TCNGA	6 cut(s)	115, 202, 276, 385, 777, 1091
Hpy188III	TCNNGA	6 cut(s)	26, 140, 476, 614, 983, 1028
Hpy8I	GTNNAC	2 cut(s)	148, 790
Hpy99I	CGWCG	2 cut(s)	10, 13
HpyAV	CCTTC	2 cut(s)	245, 646
HpyCH4III	ACNGT	2 cut(s)	380, 892
HpyCH4IV	ACGT	2 cut(s)	144, 1061
HpyCH4V	TGCA	5 cut(s)	60, 519, 557, 563, 713
HpyF10VI	GCNNNNNNNGC	6 cut(s)	11, 241, 599, 681, 751, 809
HpyF3I	CTNAG	2 cut(s)	774, 918
HpySE526I	ACGT	2 cut(s)	144, 1061
Hsp92I	GRCGYC	3 cut(s)	5, 119, 144
Hsp92II	CATG	4 cut(s)	64, 561, 717, 1036
HspAI	GCGC	2 cut(s)	188, 244
KflI	GGGWCCC	1 cut(s)	87
Kzo9I	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
LmnI	GCTCC	1 cut(s)	68
Lsp1109I	GCAGC	1 cut(s)	1063
LweI	GCATC	4 cut(s)	175, 216, 346, 790
MaeI	CTAG	3 cut(s)	134, 654, 840
MaeII	ACGT	2 cut(s)	144, 1061
MaeIII	GTNAC	3 cut(s)	851, 886, 1013
MalI	GATC	9 cut(s)	24, 161, 204, 273, 296, 480, 529, 587, 827
MboI	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
MboII	GAAGA	6 cut(s)	443, 461, 515, 722, 725, 850
MfeI	CAATTG	1 cut(s)	569
MflI	RGATCY	4 cut(s)	22, 271, 478, 527
MhlI	GDGCHC	2 cut(s)	180, 258
MluCI	AATT	5 cut(s)	569, 617, 950, 986, 1023
MlyI	GAGTC	2 cut(s)	216, 233
MmeI	TCCRAC	1 cut(s)	93
Mph1103I	ATGCAT	2 cut(s)	62, 559
MseI	TTAA	3 cut(s)	282, 498, 1085
MslI	CAYNNNNRTG	1 cut(s)	263
MspI	CCGG	3 cut(s)	72, 305, 365
MspR9I	CCNGG	3 cut(s)	196, 287, 672
MunI	CAATTG	1 cut(s)	569
MvaI	CCWGG	3 cut(s)	196, 287, 672
MvnI	CGCG	2 cut(s)	188, 244
MwoI	GCNNNNNNNGC	6 cut(s)	11, 241, 599, 681, 751, 809
NdeII	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
NlaIII	CATG	4 cut(s)	64, 561, 717, 1036
NlaIV	GGNNCC	4 cut(s)	70, 88, 89, 480
NmeAIII	GCCGAG	1 cut(s)	619
NmuCI	GTSAC	2 cut(s)	851, 1013
NsiI	ATGCAT	2 cut(s)	62, 559
NspI	RCATGY	2 cut(s)	561, 717
PaeI	GCATGC	1 cut(s)	717
PaeR7I	CTCGAG	1 cut(s)	173
PfeI	GAWTC	2 cut(s)	403, 509
PkrI	GCNGC	5 cut(s)	123, 292, 399, 767, 1053
Ple19I	CGATCG	1 cut(s)	205
PleI	GAGTC	2 cut(s)	216, 233
PpsI	GAGTC	2 cut(s)	216, 233
Ppu21I	YACGTR	1 cut(s)	1062
PpuMI	RGGWCCY	2 cut(s)	87, 130
Psp5II	RGGWCCY	2 cut(s)	87, 130
Psp6I	CCWGG	3 cut(s)	194, 285, 670
PspGI	CCWGG	3 cut(s)	194, 285, 670
PspN4I	GGNNCC	4 cut(s)	70, 88, 89, 480
PspPI	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
PspPPI	RGGWCCY	2 cut(s)	87, 130
PspXI	VCTCGAGB	1 cut(s)	173
PsuI	RGATCY	4 cut(s)	22, 271, 478, 527
PvuI	CGATCG	1 cut(s)	205
RsaI	GTAC	3 cut(s)	301, 377, 1060
RsaNI	GTAC	3 cut(s)	300, 376, 1059
RseI	CAYNNNNRTG	1 cut(s)	263
SaqAI	TTAA	3 cut(s)	282, 498, 1085
SatI	GCNGC	5 cut(s)	122, 291, 398, 766, 1052
Sau3AI	GATC	9 cut(s)	22, 159, 202, 271, 294, 478, 527, 585, 825
Sau96I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
ScaI	AGTACT	1 cut(s)	377
SchI	GAGTC	2 cut(s)	216, 233
ScrFI	CCNGG	3 cut(s)	196, 287, 672
SduI	GDGCHC	2 cut(s)	180, 258
SfaNI	GCATC	4 cut(s)	175, 216, 346, 790
SfcI	CTRYAG	1 cut(s)	340
Sfr274I	CTCGAG	1 cut(s)	173
SinI	GGWCC	5 cut(s)	87, 130, 198, 549, 650
SlaI	CTCGAG	1 cut(s)	173
SmiMI	CAYNNNNRTG	1 cut(s)	263
SmlI	CTYRAG	3 cut(s)	173, 749, 981
SmoI	CTYRAG	3 cut(s)	173, 749, 981
SnaBI	TACGTA	1 cut(s)	1062
SphI	GCATGC	1 cut(s)	717
Sse9I	AATT	5 cut(s)	569, 617, 950, 986, 1023
SsiI	CCGC	8 cut(s)	122, 290, 308, 322, 326, 397, 602, 765
SspMI	CTAG	3 cut(s)	134, 654, 840
StyD4I	CCNGG	3 cut(s)	194, 285, 670
StyI	CCWWGG	1 cut(s)	653
TaaI	ACNGT	2 cut(s)	380, 892
TaiI	ACGT	2 cut(s)	147, 1064
TaqI	TCGA	4 cut(s)	174, 249, 354, 828
TasI	AATT	5 cut(s)	569, 617, 950, 986, 1023
TatI	WGTACW	2 cut(s)	299, 375
TauI	GCSGC	4 cut(s)	124, 293, 400, 768
TfiI	GAWTC	2 cut(s)	403, 509
Tru1I	TTAA	3 cut(s)	282, 498, 1085
Tru9I	TTAA	3 cut(s)	282, 498, 1085
TseFI	GTSAC	2 cut(s)	851, 1013
TseI	GCWGC	1 cut(s)	1051
Tsp45I	GTSAC	2 cut(s)	851, 1013
TspDTI	ATGAA	6 cut(s)	70, 340, 522, 655, 897, 1059
VpaK11BI	GGWCC	5 cut(s)	87, 130, 198, 549, 650
XapI	RAATTY	3 cut(s)	617, 986, 1023
XceI	RCATGY	2 cut(s)	561, 717
XhoI	CTCGAG	1 cut(s)	173
XmaJI	CCTAGG	1 cut(s)	653
XmiI	GTMKAC	2 cut(s)	147, 789
XspI	CTAG	3 cut(s)	134, 654, 840
ZraI	GACGTC	1 cut(s)	145
ZrmI	AGTACT	1 cut(s)	377
Zsp2I	ATGCAT	2 cut(s)	62, 559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.