Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0003128

Physical Location & Seq

Genomic Coordinates Reverse (-)

34009950 .. 34018938

Gene Structure 8989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0295000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6150 bp

ATGAGCCACAAGAGAAAGTTCGATCATGATGATCCATATACCCAAATTGCTTCTTCATCTACTTCAACTAGTAGATTGGAACAACAAGAAGAAGAAGTTCCATCAGCAATGGATTACAGCAACACCGAAATAGGATCTTCATCATCATCCGACTCTTCAGCATCAGACTTGAAATTATACGATGTCTTCCTCAGTTTCAGGGGCGAGGACACCCGCCACAGTTTTACCGATCATTTGTACCATGATTTGAAGAAGATGGGAATTCTTACCTTTCGGGATGCTGAGAAACTTAGAAAAGGGGAAGCCATCGGACCAGCACTTTTGAAAGCTATTGAAGAATCAAGGTTTGCAGTGGTCATTCTTTCACCAGAATACGCTTCCTCAGCGTGGTGTTTGGATGAACTCGCCCACATTATTAAATGCAAGGAAGAGAGTGGACTGAAAGTTTATCCCGTCTTTTATCATGTCCAACCATCAGAGGTACGATGGCAGGAAACAGGAAAGTATAGTAAGGCCTTTGATGAACATGAGGAACGATACGGAGGCGCTACGGACAGAGTCACAAACTGGAGAATAGCTTTGAGAGAAGTAGCCAATCTCTCCGGATGGAATTTGGAAAACAGGTCAGAGACAGAAGTTATTCAAGAAATAGCTAGAAAGATTTCAAAAATGTTGCCCTTTATGGTCTCTAGTGCTAGCAATTATATAGTTGGAATCGATGTTCGTATAGAGACATTGAAGAATTACTTGGCTTTGTGGTATACAAATGACATTATCACCATAGGGATTTGGGGAATGGGGGGCATAGGTAAAACAACTCTTGCAGCAGAATTTTTTAAAAGATTTCGTAACCAATTTGATGATAGCGCGTTTGTTGCCAATGTTAGATTGGAAGCGCGGAAAGGTCTAGATTACTTGCGAAAACTTCTTTTTGCATCCTTTTTGGAAAGTGATGATAATAAATCGTCCGTTCAGATAGGGAATGAATTCTTAAGGAGAAGGCTAAGGAAAAAGGTTCTTATCATTTTGGACGATGTTGATGAATTAAAACAAATACAAGATTTAATAGGCGGAAGGCCAGGTGAACTTAATTGGTTGGGTCAAGGAAGTCGAGTGATTATAACAACTAGAGATAAGCAGCCGTTGTTGACATGGGGAGTCAAAGAAGAGAACATATACGAGGTTGAGAAACTGACTGATGATGAAGCTCTTCAGCTTTTATGTCAAAAAGCATTCAACAGTGAACATGCTCCAGATGATTATAAAGAGCTGTCCAACAAGTTGTTAAATTATGTTAGTGGCCTTCCTCTAGCTATTGAAGTTCTTGGTTCATACTTGTCTGGGAGACAGGAAAGTGAATGGTTGGAGGCATTGGCTAGACTTCATGAAGATCCAGAAAAAGGCATTTTCAGTGTTCTTCAAATAAGTTTTGATGGATTAAGGACAACTGAGAAGAATATCTTTTTGGATATTGCATGTTTCTTCAGAGGCGAGGATCAAGCACCTGTAAGGAAGATATTAAAAAGCTGTGGCTTTTTTCCTGAAAACGGTATAAAAGACCTTGTTTATAAATCTCTGCTCAAAATTGAAAAAAATAAACTGTGGATGCATGATTTATTGCAACAAATGGGCTGGCATATTGTTCGTAAAGAATCTCTTCAAGATCCAGGCAAACGTAGTAGGTTGTGGCTTAATGATAATACCTACTTGTACGAACGCAATGAATCGTGGCTTGACAAGGATGCCTGTGATGTACTCACAGAAAATACGGGAACGAAAGCTGTTGAAGGCATCTTCCTACGCTTGCCTGAAAAATTGGAAATCTGCTTAAATGCTGACCCGTTCTCTAATATGCGCTACCTCAGATTGTTGAAGATTTGTAATGTCAAATTTCTGGGTGCTCCTTTTATACATCTTTCTAAACACTTACGAGTTTTGGAGTGGCATGAATGTCCTTTAGAATCTCTGCCATCAAGTTGTCAACTGGACCGGCTTGTTGAACTTAAGATGCCTAATAGCCGCCTTGAACAACTATGGAATGGAAGGCTTGCATTAAAAATGCTAGTGCTAATTGATCTTTCCTACTGCCAATACTTAAGGAGGACTCTCGACTTCAGTAGGGTCCCAAATCTTGAGCGGCTAAACTTTGAAGGTTGTAAAAAATTATCAGAAGTTCACCCAACAATCGGGGATCTAAAACATCTAGTCCTATTGAATATGAAAAATTGTGAAAGTCTAGAAAGCCTTGTGAACTCCATCAGCTTGGCATCTCTTGAAACGTTTATTCTTTCCGGATGTTCAAAGCTCAAAGAGTTTCCAGAGATTGTTGGAAGAATGAAATCTTTGGCAGAACTTTACTTAGATGGGACGGCAATAAGGGAGCTGCCGATATCAATCCAACACTTGAGTGGCCTTATTTTGCTGAATTTAAAAGGCTGCAAATACCTTCTGGCTCTTCCAAGCGTCGTTTGTAATCTGATATCTCTGAAGTATCTATATCTGTCAGGCTGCTCTCGTATTGACCAACTGCCAGAACATCTAGGGAGTTTGGAACATTTGCAGGTGCTTGATGCCTGTAAAACTGCTATAAGAAAAGTTCCATCATCCATCTTACTTCTGAAGAACCCCAAACAGTTGTGTTTTCCTGGAAGTAATGATCTGCAGATGGAATTCTCTGAATCCAGTGGTTTCCGCTACTCATCCTCACATTCATGTTCATTGATGGCATTAGATATCCCCTCTGATCTTTTATGCTGTTTCTCACAAACTGATTCAGATTTGAATGAAAACAAAATGTTGATCAGCAAACCTGAAATCATCTCCGATGGATATTTGCAGGAGCTTGATACCTGGAGCACTCTGAATTGGAGTTGCAGTTTCCGTGAAACAGATTTAAGAAATAATTTTCATTTGATGCTTTGTGACAATTCTCCTCAGATGTTCTTTGATTCCTGTCAATATTCATCTTTGACATCTTTACTTCTGAGTGGATGCAAGCTAGGCGAAGGAGCAATCCCTGATGATATTGGCTGCTTATACTCACTCCATTTGTTAGACTTGAGTGAAAACAATTTCGTGAGCATACCTGAAACCATTTCTCAACTTTCCAAGCTCAGAAAGCTTCTGTTGTTTAGGTGCAGCAAACTTCAGTATTTGCCAAAACAACTTCCACTGAGTTTAAAACGTGTAATTGCACGCCATTGTCCTATGCTGAGAACTACTTATCCAAATACTATCGTAAACTGGACTTCAGATGAAGGTTTCAGTTTCATAGATTGCAGATATTCAGACGAAAATGCCGACGATGATGAAGGATATATGTCTTTCCATAAGATCATTGAGGATCGAATTCATCATGACAAGCCATTTGAAATTCGTTTTCGTCATAGTCAACTTTACCATCCTTTTCGATACAGTAGTGCTGACTTTTCTGATGAAGTTGGTGTTTCTCATGGTGATCGAATTCCGGACTGGTGCGCTCAGTGGAACACTGGTTCTTCTGTTACATTGCAACTAACTGGTCCAGAAGACAAGACTAATTGTACGTGGATGGGATTAGCCTTATTTGTTGCTTTTGAAAACATTCGGAATTATCAAGTGAAAGAAATTCTCTGTCATCTGAGCGTCGACGAAGGTGGTCCTCAAATTCCTTTAGTTTTTCAAGACTTGGATGATGACTTGGTTGATGCGTCACTTGGACTCTGTTGCTACTTGCCACGAGGCTGCGGGGGGTTTGCAGGACAGCTGGATAAAGCCAGTCGAATTAATGCTACATTTTTACCAACTACTTTACCGGTGAAATCGTGTGGGATACAGACAATATCCGAGCATGATGCTCCAATGTTTGCTCAGGATTTAACCCGGACTGCTTTTGCTGACGCACGTGTTCAAAAGGGAATTTTTTTTGCCGAGTACGCTAAGGACAGGATGGACAGGAAGGACACTGTTTATCGACATTGTAAGAGAATGTTAGATAAGGCAACGAAATTGGAAAGATCTGGAGACGTGCTGGTACCTGCGTACAATTCTTTAAATGTGAATCGACACAAGTGGAAAATTGATCACGATATCCATCCCAGATTAGAGGAAGAGCTAAAGCTGTTCAGATTGCTTTCAGGGCTCTACGAGGGAAGCAATGGACGTCAGAAGAAATTTCATTTTTGTTTTCCAGGTCCAGTAATTTCGCCCTGGTTCATCAATCACTACGCAGGGAATATAGCACTTTGTGACCTTCCTCAGAACTTAATCGATGATAAGAGATGGATTGGATTAGAAGTGTATGTTGTGTTTACTTGGCCACCAACACCTGGTTACAGTGACCACAATCTTTCTGATTCCTTCCTTCATGTTGATTTAATGGCACATGAAAGTAGTGTAATGCATTCGTCCATCAAAATTGAGAGTTGTTCTGGGTCATATCAACTTATTGTACTACATGTACCACGGATGCATTTTCCAGAACAGTTGACTCATTATCAGGTTATTAGCACGTTGTTTACAACCAGTACCCCAATTGTTGAGATTATAGTGTGCGGGAGCCGTTTGGTATATGAGCATGATCTGGAAGATTTAATCCTTTCATTAACTGCGTGTACATTGAGGAAGCCATATTCAATTGGCCAAGTTTATTCCCAGGCCCAAGTATATGTTGAAACTATGGAAAAGAAAGCAGATGAAAATATCCAGTGCAATGCAAAGGGAGGAGAAATGTCCACTGATGGCCATTCATCGTTTCAAAGAAGTGTCAAACAAATTGAAGTACCAGATAAAGTTCGCAGTTCAATATTGCAGAAATATAGATGCCATATCCAAGGAAAGCAAGGTATACTAGAGTCTGACAAAATAATTGTCCACTCTAAATCCCTTCTTCTGGACAATAGCATCAAAAAAAATTTTACTGGAAAAGAATCAATAATGTCGTCGTTGGCATGTCATCATTTGCATATAGTGGCTGAGACTTACTTCTGCGGAAGCTACAGTGCCGTACAGTGGAAGAGATTCCTAAAGTTGTTGCTGCAATATTCTGAGGTGGCAACTCTCAGTCTTAATGGACATGTAATCTCTGTAATGAAGCATTTTAGTCCCTCCTCGCCATTCAATTTTTGTTTCCCTAAAGATGAAGTGCTGCTAGACTGGATGAGAGATCGTCGATTCCTATTGCCTCAACAACTCTATGATGATAAAAGTTTGAGGGGACTTGTTATATGTGCAACTTTTTCATTTGACAATGATTCTGCCATGAACAATCTGGCTTCAGAAATGACTGTTGAACTTGTGTGTCACTTGATTGACCGTCACTCCAGGTTTTGCGTGAACGCTGCCCCCATGTTTACGATCACTAAGGAGAAATTCCAGTGGCTTTGTTTCCGTGATTTCATTTGGGTTACGTATGTACCCCTTGTTATGCTAGCGGAGGTCAAAGTAATCAGTTATATAGAGGCTGATATATGTAGTAATTGCCCAGGCTTGAGGGTGGGCTGTTCTTATACACGTGTCTTGTACCGGCAAGAGGAGGAGGAATTTAGGCAAGCAATAACTCAGTGCTGGACCTCCTTCTTTGATAATAAGGATCTCCTCCGTCGGTTTGTACAAGCAGATGCTGAAAAAGGTGTAGAGACGAGGCACGAACTTCCAATGCTCGGCGGAAGTATGAAGGACCTTGATAGCAACTTGATGTATGACGCAACACACCCATTTACTGAAATTCCGGATTGGTTTGGACATCCAAATGAAAGGTCTGGAGAGATCCAGTTACCACCATCTCTTGGCAGGGACGAAAATTGGATAGGAGTGGCTATATGTGCATCATATTCTCCCTTACAATTGAATCCTCAAGTTTCTGCGTACTTAAAATGTGTTTTGCGAACTGAGATGAGTAACTTGACATCTGTCCACCGCTATCAGATGACCAATGAAGAAGTCAAGTGCGTATCTTCTGAGAGAAAATTCATTTGGCTATCCTATCTTCCACGACACTGGTTTTTCTACCAGTTAAATGATGAGTCTGCTCTCAGGGCTTCGTTTTCAAGTTCACCTGGTTTGTGGTTTAAGGAGTGCGGTCTGCGTCTTGTATACCGGCAAGATGAGGAAGAGTTTAAGCAACTCTGTATCTCCTTAAACTCGTTGCCCGACAATCAAAAACCAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009987 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019438 GO:0034641 GO:0034654 GO:0036094 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048037 GO:0048040 GO:0048046 GO:0050662 GO:0051287 GO:0055086 GO:0071704 GO:0097159 GO:1901135 GO:1901137 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Epimerase

Protein Analysis

2049

Amino Acids

234.66

Weight (kDa)

5.96

Isoelectric Point (pI)

47.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	60 - 231	2.9e-50	TIR domain
TIR_2	PF13676	62 - 158	1.2e-14	TIR domain
NB-ARC	PF00931	255 - 415	8.4e-18	NB-ARC domain
NACHT	PF05729	263 - 409	2e-08	NACHT domain
WHD_ROQ1	PF23282	483 - 552	9.7e-19	Disease resistance protein Roq1-like, winged-helix domain
LRR_13	PF23286	755 - 840	2.4e-09	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	808 - 881	1e-10	Leucine-rich repeat region
C-JID	PF20160	1898 - 2030	1.6e-08	C-JID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08200
fragaria_vesca	FvH4_3g30060 FvH4_3g30060 FvH4_3g30060 FvH4_3g30060
malus_domestica	MD03G1131500.v1.1 MD03G1131900.v1.1 MD03G1161900.v1.1 MD11G1154500.v1.1 MD11G1215900.v1.1 MD16G1216600.v1.1 MD16G1216800.v1.1
prunus_persica	Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1
pyrus_communis	pycom11g12600 pycom11g12610
rosa_chinensis	RchiOBHm_Chr5g0055821
rosa_laevigata	RLG00000002609 RLG00000035011
rosa_multiflora	Rmu_sc0003306.1_g000005 Rmu_sc0003500.1_g000032 Rmu_sc0003500.1_g000033 Rmu_sc0007802.1_g000009
rosa_roxburghii	Rroxscaffold_1G00024520 Rroxscaffold_1G00052150
rosa_rugosa	Rorug05G0295000
rosa_samantha	Rh5AG192800 Rh5AG365900 Rh5BG190400 Rh5BG376600 Rh5CG399600 Rh5DG192100 Rh5DG389500 Rh5DG389600 Rh7BG106200
rosa_wichuraiana	Rw5G034330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1121, 1263, 1569
AarI	CACCTGC	1 cut(s)	2551
AatII	GACGTC	1 cut(s)	4144
Acc36I	ACCTGC	2 cut(s)	2551, 4024
Acc65I	GGTACC	1 cut(s)	4012
AccB1I	GGYRCC	1 cut(s)	4012
AccB7I	CCANNNNNTGG	2 cut(s)	4307, 5765
AccBSI	CCGCTC	1 cut(s)	2137
AccI	GTMKAC	4 cut(s)	761, 3627, 4825, 6072
AccII	CGCG	2 cut(s)	869, 898
AccIII	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
AclI	AACGTT	1 cut(s)	2279
AclWI	GGATC	9 cut(s)	26, 142, 1385, 1503, 1658, 2199, 3351, 5577, 5740
AcoI	YGGCCR	3 cut(s)	4295, 4618, 4720
AcuI	CTGAAG	9 cut(s)	141, 1196, 1468, 2098, 2507, 2639, 3131, 3234, 5238
AcvI	CACGTG	2 cut(s)	3884, 5492
AcyI	GRCGYC	1 cut(s)	4141
AdeI	CACNNNGTG	1 cut(s)	4226
AflII	CTTAAG	3 cut(s)	991, 2003, 2095
AflIII	ACRYGT	6 cut(s)	3184, 3883, 4435, 5053, 5489, 5491
AgeI	ACCGGT	1 cut(s)	3793
AhlI	ACTAGT	1 cut(s)	68
AjiI	CACGTC	1 cut(s)	4006
AjuI	GAANNNNNNNTTGG	6 cut(s)	731, 763, 1448, 1480, 5935, 5967
AleI	CACNNNNGTG	1 cut(s)	2406
AloI	GAACNNNNNNTCC	6 cut(s)	705, 737, 3208, 3240, 3478, 3510
Alw21I	GWGCWC	2 cut(s)	1903, 2858
Alw26I	GTCTC	7 cut(s)	623, 691, 725, 1339, 3996, 4949, 5609
AlwI	GGATC	9 cut(s)	26, 142, 1385, 1503, 1658, 2199, 3351, 5577, 5740
AlwNI	CAGNNNCTG	1 cut(s)	5600
Aor13HI	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
AoxI	GGCC	8 cut(s)	513, 1076, 1300, 2410, 4295, 4618, 4635, 4720
ArsI	GACNNNNNNTTYG	6 cut(s)	3350, 3382, 4833, 4865, 5720, 5752
AseI	ATTAAT	1 cut(s)	3765
AsiGI	ACCGGT	1 cut(s)	3793
Asp700I	GAANNNNTTC	8 cut(s)	96, 639, 661, 986, 1209, 1656, 3582, 4997
Asp718I	GGTACC	1 cut(s)	4012
AspLEI	GCGC	5 cut(s)	548, 869, 898, 1857, 3479
AspS9I	GGNCC	9 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 4636, 5547, 5656
AsuC2I	CCSGG	1 cut(s)	3862
AsuHPI	GGTGA	7 cut(s)	357, 769, 1094, 2168, 3467, 3808, 6024
AsuNHI	GCTAGC	2 cut(s)	695, 5407
AvaII	GGWCC	8 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 5547, 5656
BaeI	ACNNNNGTAYC	6 cut(s)	3403, 3436, 4004, 4037, 5376, 5409
BalI	TGGCCA	3 cut(s)	4297, 4620, 4722
BanI	GGYRCC	1 cut(s)	4012
BanII	GRGCYC	1 cut(s)	4122
BauI	CACGAG	1 cut(s)	3717
BbrPI	CACGTG	2 cut(s)	3884, 5492
BbsI	GAAGAC	2 cut(s)	178, 3534
Bbv12I	GWGCWC	2 cut(s)	1903, 2858
BbvCI	CCTCAGC	1 cut(s)	382
BceAI	ACGGC	4 cut(s)	1126, 2385, 4524, 4967
BcgI	CGANNNNNNTGC	4 cut(s)	1625, 1659, 3815, 3849
BciVI	GTATCC	1 cut(s)	3804
BclI	TGATCA	2 cut(s)	2799, 4060
BcnI	CCSGG	1 cut(s)	3862
BcoDI	GTCTC	7 cut(s)	623, 691, 725, 1339, 3996, 4949, 5609
BcuI	ACTAGT	1 cut(s)	68
BfmI	CTRYAG	2 cut(s)	2660, 4975
BfoI	RGCGCY	1 cut(s)	549
BfrI	CTTAAG	3 cut(s)	991, 2003, 2095
BfuAI	ACCTGC	2 cut(s)	2551, 4024
BfuI	GTATCC	1 cut(s)	3804
BglII	AGATCT	1 cut(s)	3995
Bme18I	GGWCC	8 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 5547, 5656
BmgBI	CACGTC	1 cut(s)	4006
BmgT120I	GGNCC	9 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 4636, 5547, 5656
BmiI	GGNNCC	4 cut(s)	2123, 2124, 4014, 4538
BmtI	GCTAGC	2 cut(s)	699, 5411
BoxI	GACNNNNGTC	1 cut(s)	5888
BpiI	GAAGAC	2 cut(s)	178, 3534
BpmI	CTGGAG	6 cut(s)	589, 1236, 2872, 4020, 5284, 5760
Bpu10I	CCTNAGC	4 cut(s)	382, 1004, 3849, 3918
BpuEI	CTTGAG	5 cut(s)	2153, 2425, 3079, 5488, 5817
BpuMI	CCSGG	1 cut(s)	3862
Bsa29I	ATCGAT	2 cut(s)	717, 4248
BsaAI	YACGTR	4 cut(s)	3546, 3884, 5388, 5492
BsaBI	GATNNNNATC	3 cut(s)	139, 2393, 4071
BsaHI	GRCGYC	1 cut(s)	4141
BsaI	GGTCTC	1 cut(s)	691
BsaJI	CCNNGG	5 cut(s)	4187, 4442, 4632, 4810, 5461
BsaWI	WCCGGW	5 cut(s)	602, 2291, 3466, 3793, 5707
BsaXI	ACNNNNNCTCC	4 cut(s)	2860, 2890, 5282, 5312
Bse118I	RCCGGY	4 cut(s)	1989, 3793, 5502, 6075
Bse3DI	GCAATG	5 cut(s)	114, 1726, 3506, 4141, 4696
Bse8I	GATNNNNATC	3 cut(s)	139, 2393, 4071
BseAI	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
BseCI	ATCGAT	2 cut(s)	717, 4248
BseDI	CCNNGG	5 cut(s)	4187, 4442, 4632, 4810, 5461
BseJI	GATNNNNATC	3 cut(s)	139, 2393, 4071
BseMI	GCAATG	5 cut(s)	114, 1726, 3506, 4141, 4696
BseRI	GAGGAG	6 cut(s)	2922, 4716, 5077, 5525, 5528, 5564
BsgI	GTGCAG	1 cut(s)	3157
Bsh1236I	CGCG	2 cut(s)	869, 898
BshFI	GGCC	8 cut(s)	515, 1078, 1302, 2412, 4297, 4620, 4637, 4722
BshNI	GGYRCC	1 cut(s)	4012
BshTI	ACCGGT	1 cut(s)	3793
BshVI	ATCGAT	2 cut(s)	717, 4248
BsiHKAI	GWGCWC	2 cut(s)	1903, 2858
BsiSI	CCGG	9 cut(s)	603, 1990, 2292, 3467, 3794, 3862, 5503, 5708, 6076
BslFI	GGGAC	5 cut(s)	2108, 2380, 5067, 5208, 5786
BsmAI	GTCTC	7 cut(s)	623, 691, 725, 1339, 3996, 4949, 5609
BsmBI	CGTCTC	2 cut(s)	3996, 5609
BsmFI	GGGAC	5 cut(s)	2108, 2380, 5067, 5208, 5786
BsmI	GAATGC	2 cut(s)	1232, 4381
BsnI	GGCC	8 cut(s)	515, 1078, 1302, 2412, 4297, 4620, 4637, 4722
Bso31I	GGTCTC	1 cut(s)	691
Bsp1286I	GDGCHC	3 cut(s)	1903, 2858, 4122
Bsp13I	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
Bsp1407I	TGTACA	2 cut(s)	4592, 5587
BspANI	GGCC	8 cut(s)	515, 1078, 1302, 2412, 4297, 4620, 4637, 4722
BspDI	ATCGAT	2 cut(s)	717, 4248
BspEI	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
BspFNI	CGCG	2 cut(s)	869, 898
BspHI	TCATGA	3 cut(s)	25, 1384, 3355
BspLI	GGNNCC	4 cut(s)	2123, 2124, 4014, 4538
BspMAI	CTGCAG	1 cut(s)	2664
BspMI	ACCTGC	2 cut(s)	2551, 4024
BspOI	GCTAGC	2 cut(s)	699, 5411
BspPI	GGATC	9 cut(s)	26, 142, 1385, 1503, 1658, 2199, 3351, 5577, 5740
BspQI	GCTCTTC	3 cut(s)	1215, 2460, 4083
BspT107I	GGYRCC	1 cut(s)	4012
BspTI	CTTAAG	3 cut(s)	991, 2003, 2095
BspTNI	GGTCTC	1 cut(s)	691
BsrBI	CCGCTC	1 cut(s)	2137
BsrDI	GCAATG	5 cut(s)	114, 1726, 3506, 4141, 4696
BsrFI	RCCGGY	4 cut(s)	1989, 3793, 5502, 6075
BsrGI	TGTACA	2 cut(s)	4592, 5587
BssAI	RCCGGY	4 cut(s)	1989, 3793, 5502, 6075
BssECI	CCNNGG	5 cut(s)	4187, 4442, 4632, 4810, 5461
BssNAI	GTATAC	3 cut(s)	762, 4826, 6073
BssNI	GRCGYC	1 cut(s)	4141
BssSI	CACGAG	1 cut(s)	3717
BssT1I	CCWWGG	1 cut(s)	4810
Bst1107I	GTATAC	3 cut(s)	762, 4826, 6073
Bst2BI	CACGAG	1 cut(s)	3717
Bst6I	CTCTTC	9 cut(s)	160, 423, 1161, 1215, 1662, 2460, 4083, 4988, 6084
BstACI	GRCGYC	1 cut(s)	4141
BstAFI	CTTAAG	3 cut(s)	991, 2003, 2095
BstAUI	TGTACA	2 cut(s)	4592, 5587
BstBAI	YACGTR	4 cut(s)	3546, 3884, 5388, 5492
BstC8I	GCNNGC	8 cut(s)	697, 1634, 1805, 2049, 2996, 3196, 5409, 5529
BstDSI	CCRYGG	1 cut(s)	4442
BstFNI	CGCG	2 cut(s)	869, 898
BstH2I	RGCGCY	1 cut(s)	549
BstHHI	GCGC	5 cut(s)	548, 869, 898, 1857, 3479
BstMAI	GTCTC	7 cut(s)	623, 691, 725, 1339, 3996, 4949, 5609
BstMWI	GCNNNNNNNGC	8 cut(s)	383, 875, 3000, 3118, 3914, 4117, 5801, 6014
BstNSI	RCATGY	5 cut(s)	1250, 1479, 4439, 4932, 5057
BstPAI	GACNNNNGTC	1 cut(s)	5888
BstSFI	CTRYAG	2 cut(s)	2660, 4975
BstSNI	TACGTA	1 cut(s)	5388
BstUI	CGCG	2 cut(s)	869, 898
BstV2I	GAAGAC	2 cut(s)	178, 3534
BstX2I	RGATCY	7 cut(s)	134, 1390, 1663, 2191, 3995, 5569, 5745
BstXI	CCANNNNNNTGG	2 cut(s)	2263, 5976
BstYI	RGATCY	7 cut(s)	134, 1390, 1663, 2191, 3995, 5569, 5745
BstZ17I	GTATAC	3 cut(s)	762, 4826, 6073
Bsu15I	ATCGAT	2 cut(s)	717, 4248
BsuI	GTATCC	1 cut(s)	3804
BsuRI	GGCC	8 cut(s)	515, 1078, 1302, 2412, 4297, 4620, 4637, 4722
BsuTUI	ATCGAT	2 cut(s)	717, 4248
BtgI	CCRYGG	1 cut(s)	4442
BtrI	CACGTC	1 cut(s)	4006
BtsI	GCAGTG	1 cut(s)	357
BveI	ACCTGC	2 cut(s)	2551, 4024
Cac8I	GCNNGC	8 cut(s)	697, 1634, 1805, 2049, 2996, 3196, 5409, 5529
CaiI	CAGNNNCTG	1 cut(s)	5600
CciI	TCATGA	3 cut(s)	25, 1384, 3355
CfoI	GCGC	5 cut(s)	548, 869, 898, 1857, 3479
Cfr10I	RCCGGY	4 cut(s)	1989, 3793, 5502, 6075
Cfr13I	GGNCC	9 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 4636, 5547, 5656
ClaI	ATCGAT	2 cut(s)	717, 4248
CseI	GACGC	6 cut(s)	2452, 3613, 3678, 3887, 5690, 6053
CsiI	ACCWGGT	2 cut(s)	4306, 6034
CspAI	ACCGGT	1 cut(s)	3793
DraI	TTTAAA	4 cut(s)	838, 2430, 3180, 4032
DraIII	CACNNNGTG	1 cut(s)	4226
EaeI	YGGCCR	3 cut(s)	4295, 4618, 4720
Eam1104I	CTCTTC	9 cut(s)	160, 423, 1161, 1215, 1662, 2460, 4083, 4988, 6084
EarI	CTCTTC	9 cut(s)	160, 423, 1161, 1215, 1662, 2460, 4083, 4988, 6084
EciI	GGCGGA	2 cut(s)	1086, 5658
Eco105I	TACGTA	1 cut(s)	5388
Eco130I	CCWWGG	1 cut(s)	4810
Eco147I	AGGCCT	1 cut(s)	515
Eco24I	GRGCYC	1 cut(s)	4122
Eco31I	GGTCTC	1 cut(s)	691
Eco32I	GATATC	4 cut(s)	2391, 2481, 2734, 4069
Eco47I	GGWCC	8 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 5547, 5656
Eco57I	CTGAAG	9 cut(s)	141, 1196, 1468, 2098, 2507, 2639, 3131, 3234, 5238
Eco72I	CACGTG	2 cut(s)	3884, 5492
EcoO109I	RGGNCCY	2 cut(s)	2122, 5656
EcoRI	GAATTC	5 cut(s)	261, 986, 2669, 3348, 3462
EcoRV	GATATC	4 cut(s)	2391, 2481, 2734, 4069
EcoT14I	CCWWGG	1 cut(s)	4810
EcoT22I	ATGCAT	3 cut(s)	1611, 4383, 4452
EcoT38I	GRGCYC	1 cut(s)	4122
ErhI	CCWWGG	1 cut(s)	4810
Esp3I	CGTCTC	2 cut(s)	3996, 5609
FalI	AAGNNNNNCTT	6 cut(s)	731, 763, 1445, 1477, 1641, 1673
FaqI	GGGAC	5 cut(s)	2108, 2380, 5067, 5208, 5786
FauI	CCCGC	3 cut(s)	221, 3719, 4526
FbaI	TGATCA	2 cut(s)	2799, 4060
FblI	GTMKAC	4 cut(s)	761, 3627, 4825, 6072
FriOI	GRGCYC	1 cut(s)	4122
GlaI	GCGC	5 cut(s)	547, 868, 897, 1856, 3478
GsuI	CTGGAG	6 cut(s)	589, 1236, 2872, 4020, 5284, 5760
HaeII	RGCGCY	1 cut(s)	549
HaeIII	GGCC	8 cut(s)	515, 1078, 1302, 2412, 4297, 4620, 4637, 4722
HapII	CCGG	9 cut(s)	603, 1990, 2292, 3467, 3794, 3862, 5503, 5708, 6076
HgaI	GACGC	6 cut(s)	2452, 3613, 3678, 3887, 5690, 6053
HhaI	GCGC	5 cut(s)	548, 869, 898, 1857, 3479
Hin1I	GRCGYC	1 cut(s)	4141
Hin6I	GCGC	5 cut(s)	546, 867, 896, 1855, 3477
HinP1I	GCGC	5 cut(s)	546, 867, 896, 1855, 3477
HincII	GTYRAC	5 cut(s)	1149, 1982, 3392, 3628, 4467
HindII	GTYRAC	5 cut(s)	1149, 1982, 3392, 3628, 4467
HindIII	AAGCTT	1 cut(s)	3119
HpaII	CCGG	9 cut(s)	603, 1990, 2292, 3467, 3794, 3862, 5503, 5708, 6076
HphI	GGTGA	7 cut(s)	357, 769, 1094, 2168, 3467, 3808, 6024
Hpy99I	CGWCG	7 cut(s)	2468, 3305, 3629, 3632, 4924, 5151, 5583
HpyF10VI	GCNNNNNNNGC	8 cut(s)	383, 875, 3000, 3118, 3914, 4117, 5801, 6014
Hsp92I	GRCGYC	1 cut(s)	4141
HspAI	GCGC	5 cut(s)	546, 867, 896, 1855, 3477
KflI	GGGWCCC	1 cut(s)	2122
Kpn2I	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
KpnI	GGTACC	1 cut(s)	4016
Ksp22I	TGATCA	2 cut(s)	2799, 4060
LguI	GCTCTTC	3 cut(s)	1215, 2460, 4083
LmnI	GCTCC	8 cut(s)	1255, 1906, 2380, 2839, 2853, 3008, 3841, 4536
MabI	ACCWGGT	2 cut(s)	4306, 6034
MbiI	CCGCTC	1 cut(s)	2137
MfeI	CAATTG	3 cut(s)	4512, 4614, 5822
MflI	RGATCY	7 cut(s)	134, 1390, 1663, 2191, 3995, 5569, 5745
MhlI	GDGCHC	3 cut(s)	1903, 2858, 4122
MlsI	TGGCCA	3 cut(s)	4297, 4620, 4722
MluNI	TGGCCA	3 cut(s)	4297, 4620, 4722
MlyI	GAGTC	8 cut(s)	146, 567, 1167, 2098, 3693, 4462, 4841, 6011
MmeI	TCCRAC	7 cut(s)	174, 493, 691, 1299, 1344, 2308, 2422
Mox20I	TGGCCA	3 cut(s)	4297, 4620, 4722
Mph1103I	ATGCAT	3 cut(s)	1611, 4383, 4452
MroI	TCCGGA	4 cut(s)	602, 2291, 3466, 5707
MroXI	GAANNNNTTC	8 cut(s)	96, 639, 661, 986, 1209, 1656, 3582, 4997
MscI	TGGCCA	3 cut(s)	4297, 4620, 4722
MslI	CAYNNNNRTG	3 cut(s)	2406, 2710, 5727
Msp20I	TGGCCA	3 cut(s)	4297, 4620, 4722
MspA1I	CMGCKG	1 cut(s)	3745
MspCI	CTTAAG	3 cut(s)	991, 2003, 2095
MspI	CCGG	9 cut(s)	603, 1990, 2292, 3467, 3794, 3862, 5503, 5708, 6076
MunI	CAATTG	3 cut(s)	4512, 4614, 5822
Mva1269I	GAATGC	2 cut(s)	1232, 4381
MvnI	CGCG	2 cut(s)	869, 898
MwoI	GCNNNNNNNGC	8 cut(s)	383, 875, 3000, 3118, 3914, 4117, 5801, 6014
NciI	CCSGG	1 cut(s)	3862
NheI	GCTAGC	2 cut(s)	695, 5407
NlaIV	GGNNCC	4 cut(s)	2123, 2124, 4014, 4538
NmeAIII	GCCGAG	2 cut(s)	3934, 5619
NmuCI	GTSAC	7 cut(s)	559, 2921, 3690, 4226, 4316, 5279, 5294
NsiI	ATGCAT	3 cut(s)	1611, 4383, 4452
NspI	RCATGY	5 cut(s)	1250, 1479, 4439, 4932, 5057
OliI	CACNNNNGTG	1 cut(s)	2406
PagI	TCATGA	3 cut(s)	25, 1384, 3355
PaqCI	CACCTGC	1 cut(s)	2551
PceI	AGGCCT	1 cut(s)	515
PciI	ACATGT	2 cut(s)	4435, 5053
PciSI	GCTCTTC	3 cut(s)	1215, 2460, 4083
PcsI	WCGNNNNNNNCGW	1 cut(s)	3297
PctI	GAATGC	2 cut(s)	1232, 4381
PdmI	GAANNNNTTC	8 cut(s)	96, 639, 661, 986, 1209, 1656, 3582, 4997
PflFI	GACNNNGTC	1 cut(s)	557
PflMI	CCANNNNNTGG	2 cut(s)	4307, 5765
PfoI	TCCNGGA	1 cut(s)	2644
PinAI	ACCGGT	1 cut(s)	3793
PleI	GAGTC	8 cut(s)	146, 566, 1166, 2098, 3693, 4462, 4840, 6010
PmaCI	CACGTG	2 cut(s)	3884, 5492
PmlI	CACGTG	2 cut(s)	3884, 5492
PpsI	GAGTC	8 cut(s)	146, 566, 1166, 2098, 3693, 4462, 4840, 6010
Ppu21I	YACGTR	4 cut(s)	3546, 3884, 5388, 5492
PpuMI	RGGWCCY	2 cut(s)	2122, 5656
PscI	ACATGT	2 cut(s)	4435, 5053
PshAI	GACNNNNGTC	1 cut(s)	5888
PshBI	ATTAAT	1 cut(s)	3765
PsiI	TTATAA	3 cut(s)	1121, 1263, 1569
Psp1406I	AACGTT	1 cut(s)	2279
Psp5II	RGGWCCY	2 cut(s)	2122, 5656
PspCI	CACGTG	2 cut(s)	3884, 5492
PspN4I	GGNNCC	4 cut(s)	2123, 2124, 4014, 4538
PspPI	GGNCC	9 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 4636, 5547, 5656
PspPPI	RGGWCCY	2 cut(s)	2122, 5656
PstI	CTGCAG	1 cut(s)	2664
PstNI	CAGNNNCTG	1 cut(s)	5600
PsuI	RGATCY	7 cut(s)	134, 1390, 1663, 2191, 3995, 5569, 5745
PsyI	GACNNNGTC	1 cut(s)	557
PvuII	CAGCTG	1 cut(s)	3745
RseI	CAYNNNNRTG	3 cut(s)	2406, 2710, 5727
SalI	GTCGAC	1 cut(s)	3626
SapI	GCTCTTC	3 cut(s)	1215, 2460, 4083
Sau96I	GGNCC	9 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 4636, 5547, 5656
SchI	GAGTC	8 cut(s)	146, 567, 1167, 2098, 3693, 4462, 4841, 6011
SduI	GDGCHC	3 cut(s)	1903, 2858, 4122
SexAI	ACCWGGT	2 cut(s)	4306, 6034
SfcI	CTRYAG	2 cut(s)	2660, 4975
SgrDI	CGTCGACG	1 cut(s)	3626
SinI	GGWCC	8 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 5547, 5656
SmiMI	CAYNNNNRTG	3 cut(s)	2406, 2710, 5727
SmlI	CTYRAG	8 cut(s)	991, 2003, 2095, 2132, 2404, 3058, 5467, 5832
SmoI	CTYRAG	8 cut(s)	991, 2003, 2095, 2132, 2404, 3058, 5467, 5832
SnaBI	TACGTA	1 cut(s)	5388
SpeI	ACTAGT	1 cut(s)	68
SseBI	AGGCCT	1 cut(s)	515
SspI	AATATT	3 cut(s)	2960, 4785, 5021
StuI	AGGCCT	1 cut(s)	515
StyI	CCWWGG	1 cut(s)	4810
TatI	WGTACW	4 cut(s)	1753, 4429, 4592, 5587
TauI	GCSGC	2 cut(s)	2022, 2140
TseFI	GTSAC	7 cut(s)	559, 2921, 3690, 4226, 4316, 5279, 5294
Tsp45I	GTSAC	7 cut(s)	559, 2921, 3690, 4226, 4316, 5279, 5294
TspGWI	ACGGA	7 cut(s)	555, 566, 958, 2870, 4459, 5357, 5567
Tth111I	GACNNNGTC	1 cut(s)	557
Van91I	CCANNNNNTGG	2 cut(s)	4307, 5765
Vha464I	CTTAAG	3 cut(s)	991, 2003, 2095
VpaK11BI	GGWCC	8 cut(s)	311, 1987, 2122, 3521, 3638, 4172, 5547, 5656
VspI	ATTAAT	1 cut(s)	3765
XbaI	TCTAGA	2 cut(s)	907, 2236
XceI	RCATGY	5 cut(s)	1250, 1479, 4439, 4932, 5057
XcmI	CCANNNNNNNNNTGG	3 cut(s)	886, 2405, 5245
XmiI	GTMKAC	4 cut(s)	761, 3627, 4825, 6072
XmnI	GAANNNNTTC	8 cut(s)	96, 639, 661, 986, 1209, 1656, 3582, 4997
ZraI	GACGTC	1 cut(s)	4142
Zsp2I	ATGCAT	3 cut(s)	1611, 4383, 4452

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.