Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003128

Physical Location & Seq

Genomic Coordinates Forward (+)

14833934 .. 14837657

Gene Structure 3724 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1154500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1170 bp

ATGGCGTCATCGGCGAGAGTAGATCTAGACGGCAACCCGATAAAGCCCATTACGATATGTATGATCGGAGCCGGAGGGTTCATTGGGTCCCACCTCTGCGAGAAGCTGATGTCGGAGACGCCGCACAAGGTCCTGGCTCTGGACGTCTACAATGACAAGATCAAGCACCTGCTTGAGCCCGAGAACGCGCACCCCTGGTCCGATCGTATCCAGTTCCACCGACTCAACATCAAGCAGGACTCGCGCCTCGAAGGCCTCATCAAAATGGCAGATCTGACGATTAATCTGGCGGCCATCTGTACTCCGGCAGACTACAACACCCGTCCGCTCGACACCATCTACAGCAACTTCATCGATGCTCTGCCGGTGGTGAAGTACTGCTCCGAAAACAACAAGCGTTTGATTCACTTTTCTACTTGTGAAGTGTATGGGAAAACCATTGGAAGCTATCTTCCTAAAGATAGCCCTCTTCGTCAGGATCCTGCTTATTATGTGCTTAAAGAAGATGAATCCCCTTGCATTTTTGGATCTATTGAGAAGCAGAGATGGTCCTATGCATGCGCAAAGCAATTGATCGAGAGGCTGATTTATGCTGAGGGTGCGGAAAATGGTCTTGAGTTTACCATTGTGAGACCCTTTAACTGGATTGGACCTAGGATGGATTTCATTCCTGGCATTGACGGCCCAAGTGAGGGTGTTCCAAGAGTTCTTGCATGCTTCAGTAATAATCTTCTTCGCCGTGAGCCTCTCAAGCTTGTAGATGGTGGCCAATCTCAGAGAACTTTTGTCTACATAAAGGATGCTATTGAAGCTGTTATGTTGATGATCGAAAATCCTGCTAGGGCCAATGGTCACATCTTCAATGTTGGCAACCCTAACAATGAAGTTACTGTTAGGCAGCTTGGTGAAATGATGACTGAGGTTTATGTGAAAGTAAGTGGCGAACCAAAACTGGAGACACCCACCATTGATGTTAGCTCTCAAGAATTCTATGGAGTGGGATATGATGATAGTGACAAGAGAATACCTGACATGACTATTATCAACAAGCAGCTTGGTTGGAATCCAAAGACCTCTCTCTGGGACTTGCTTGAATCAACCCTCACCTATCAGCATAGGACATATGCCGAGGCTGTCAAGAAGGCCATTTCCCAATCATCTGCAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009987 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019438 GO:0034641 GO:0034654 GO:0036094 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048037 GO:0048040 GO:0048046 GO:0050662 GO:0051287 GO:0055086 GO:0071704 GO:0097159 GO:1901135 GO:1901137 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Epimerase

Protein Analysis

390

Amino Acids

43.74

Weight (kDa)

5.71

Isoelectric Point (pI)

48.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	19 - 290	9.5e-48	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	20 - 139	2.4e-06	Polysaccharide biosynthesis protein
3Beta_HSD	PF01073	21 - 145	5.4e-06	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	23 - 366	1.1e-27	GDP-mannose 4,6 dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08200
fragaria_vesca	FvH4_3g30060 FvH4_3g30060 FvH4_3g30060 FvH4_3g30060
malus_domestica	MD03G1131500.v1.1 MD03G1131900.v1.1 MD03G1161900.v1.1 MD11G1154500.v1.1 MD11G1215900.v1.1 MD16G1216600.v1.1 MD16G1216800.v1.1
prunus_persica	Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1
pyrus_communis	pycom11g12600 pycom11g12610
rosa_chinensis	RchiOBHm_Chr5g0055821
rosa_laevigata	RLG00000002609 RLG00000035011
rosa_multiflora	Rmu_sc0003306.1_g000005 Rmu_sc0003500.1_g000032 Rmu_sc0003500.1_g000033 Rmu_sc0007802.1_g000009
rosa_roxburghii	Rroxscaffold_1G00024520 Rroxscaffold_1G00052150
rosa_rugosa	Rorug05G0295000
rosa_samantha	Rh5AG192800 Rh5AG365900 Rh5BG190400 Rh5BG376600 Rh5CG399600 Rh5DG192100 Rh5DG389500 Rh5DG389600 Rh7BG106200
rosa_wichuraiana	Rw5G034330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	177
AatII	GACGTC	1 cut(s)	147
Acc16I	TGCGCA	1 cut(s)	562
Acc36I	ACCTGC	1 cut(s)	177
AccBSI	CCGCTC	1 cut(s)	328
AccI	GTMKAC	2 cut(s)	147, 789
AccII	CGCG	2 cut(s)	188, 244
AciI	CCGC	4 cut(s)	122, 290, 326, 602
AclWI	GGATC	3 cut(s)	473, 486, 535
AcoI	YGGCCR	2 cut(s)	291, 766
AcsI	RAATTY	1 cut(s)	986
AcuI	CTGAAG	1 cut(s)	703
AcyI	GRCGYC	3 cut(s)	5, 119, 144
AfaI	GTAC	2 cut(s)	301, 377
AfiI	CCNNNNNNNGG	5 cut(s)	139, 642, 691, 692, 1080
AgsI	TTSAA	3 cut(s)	809, 862, 1094
AjnI	CCWGG	3 cut(s)	132, 194, 670
AluBI	AGCT	8 cut(s)	106, 447, 754, 812, 901, 978, 1054, 1167
AluI	AGCT	8 cut(s)	106, 447, 754, 812, 901, 978, 1054, 1167
Alw26I	GTCTC	3 cut(s)	110, 625, 950
AlwI	GGATC	3 cut(s)	473, 486, 535
Ama87I	CYCGRG	1 cut(s)	179
AoxI	GGCC	6 cut(s)	253, 291, 682, 766, 843, 1143
ApeKI	GCWGC	2 cut(s)	898, 1051
ApoI	RAATTY	1 cut(s)	986
AseI	ATTAAT	1 cut(s)	282
AspA2I	CCTAGG	1 cut(s)	653
AspLEI	GCGC	3 cut(s)	190, 246, 563
AspS9I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
AsuHPI	GGTGA	3 cut(s)	382, 917, 1096
AvaI	CYCGRG	1 cut(s)	179
AvaII	GGWCC	5 cut(s)	87, 130, 198, 549, 650
AvrII	CCTAGG	1 cut(s)	653
BalI	TGGCCA	1 cut(s)	768
BamHI	GGATCC	1 cut(s)	478
BanII	GRGCYC	1 cut(s)	180
BbvCI	CCTCAGC	1 cut(s)	594
BbvI	GCAGC	2 cut(s)	910, 1063
BccI	CCATC	5 cut(s)	302, 344, 540, 652, 755
BceAI	ACGGC	3 cut(s)	46, 697, 723
BcgI	CGANNNNNNTGC	4 cut(s)	334, 368, 818, 852
BciT130I	CCWGG	3 cut(s)	134, 196, 672
BciVI	GTATCC	1 cut(s)	218
BcoDI	GTCTC	3 cut(s)	110, 625, 950
BfaI	CTAG	3 cut(s)	26, 654, 840
BfmI	CTRYAG	1 cut(s)	340
BfuAI	ACCTGC	1 cut(s)	177
BfuI	GTATCC	1 cut(s)	218
BglI	GCCNNNNNGGC	1 cut(s)	252
BglII	AGATCT	2 cut(s)	22, 271
BisI	GCNGC	4 cut(s)	122, 291, 899, 1052
BlnI	CCTAGG	1 cut(s)	653
BlsI	GCNGC	4 cut(s)	123, 292, 900, 1053
BmcAI	AGTACT	1 cut(s)	377
Bme1390I	CCNGG	3 cut(s)	134, 196, 672
Bme18I	GGWCC	5 cut(s)	87, 130, 198, 549, 650
BmeT110I	CYCGRG	1 cut(s)	179
BmgT120I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
BmiI	GGNNCC	4 cut(s)	70, 88, 89, 480
BmrFI	CCNGG	3 cut(s)	134, 196, 672
BmsI	GCATC	2 cut(s)	346, 790
BpmI	CTGGAG	1 cut(s)	974
Bpu10I	CCTNAGC	1 cut(s)	594
BpuEI	CTTGAG	4 cut(s)	194, 635, 734, 966
Bsa29I	ATCGAT	1 cut(s)	354
BsaHI	GRCGYC	3 cut(s)	5, 119, 144
BsaI	GGTCTC	1 cut(s)	625
BsaJI	CCNNGG	3 cut(s)	194, 653, 1128
Bsc4I	CCNNNNNNNGG	5 cut(s)	139, 642, 691, 692, 1080
Bse118I	RCCGGY	1 cut(s)	364
Bse1I	ACTGG	3 cut(s)	211, 647, 957
BseBI	CCWGG	3 cut(s)	134, 196, 672
BseCI	ATCGAT	1 cut(s)	354
BseDI	CCNNGG	3 cut(s)	194, 653, 1128
BseGI	GGATG	2 cut(s)	663, 805
BseLI	CCNNNNNNNGG	5 cut(s)	139, 642, 691, 692, 1080
BseMII	CTCAG	3 cut(s)	585, 788, 909
BseNI	ACTGG	3 cut(s)	211, 647, 957
BseXI	GCAGC	2 cut(s)	910, 1063
Bsh1236I	CGCG	2 cut(s)	188, 244
Bsh1285I	CGRYCG	1 cut(s)	205
BshFI	GGCC	6 cut(s)	255, 293, 684, 768, 845, 1145
BshVI	ATCGAT	1 cut(s)	354
BsiEI	CGRYCG	1 cut(s)	205
BsiHKCI	CYCGRG	1 cut(s)	179
BsiSI	CCGG	3 cut(s)	72, 305, 365
BslFI	GGGAC	2 cut(s)	73, 1097
BslI	CCNNNNNNNGG	5 cut(s)	139, 642, 691, 692, 1080
BsmAI	GTCTC	3 cut(s)	110, 625, 950
BsmBI	CGTCTC	1 cut(s)	110
BsmFI	GGGAC	2 cut(s)	73, 1097
BsnI	GGCC	6 cut(s)	255, 293, 684, 768, 845, 1145
Bso31I	GGTCTC	1 cut(s)	625
BsoBI	CYCGRG	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	180
Bsp143I	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
BspACI	CCGC	4 cut(s)	122, 290, 326, 602
BspANI	GGCC	6 cut(s)	255, 293, 684, 768, 845, 1145
BspCNI	CTCAG	3 cut(s)	586, 787, 910
BspDI	ATCGAT	1 cut(s)	354
BspFNI	CGCG	2 cut(s)	188, 244
BspLI	GGNNCC	4 cut(s)	70, 88, 89, 480
BspMI	ACCTGC	1 cut(s)	177
BspPI	GGATC	3 cut(s)	473, 486, 535
BspTNI	GGTCTC	1 cut(s)	625
BsrBI	CCGCTC	1 cut(s)	328
BsrFI	RCCGGY	1 cut(s)	364
BsrI	ACTGG	3 cut(s)	211, 647, 957
BssAI	RCCGGY	1 cut(s)	364
BssECI	CCNNGG	3 cut(s)	194, 653, 1128
BssMI	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
BssNI	GRCGYC	3 cut(s)	5, 119, 144
BssT1I	CCWWGG	1 cut(s)	653
Bst2UI	CCWGG	3 cut(s)	134, 196, 672
Bst4CI	ACNGT	1 cut(s)	892
Bst6I	CTCTTC	1 cut(s)	474
BstACI	GRCGYC	3 cut(s)	5, 119, 144
BstC8I	GCNNGC	3 cut(s)	559, 715, 1165
BstDEI	CTNAG	3 cut(s)	594, 774, 918
BstF5I	GGATG	2 cut(s)	663, 805
BstFNI	CGCG	2 cut(s)	188, 244
BstHHI	GCGC	3 cut(s)	190, 246, 563
BstKTI	GATC	9 cut(s)	25, 66, 162, 205, 274, 481, 530, 576, 828
BstMAI	GTCTC	3 cut(s)	110, 625, 950
BstMBI	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
BstMCI	CGRYCG	1 cut(s)	205
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 241, 252, 599, 681, 751, 809
BstNI	CCWGG	3 cut(s)	134, 196, 672
BstNSI	RCATGY	2 cut(s)	561, 717
BstSCI	CCNGG	3 cut(s)	132, 194, 670
BstSFI	CTRYAG	1 cut(s)	340
BstUI	CGCG	2 cut(s)	188, 244
BstV1I	GCAGC	2 cut(s)	910, 1063
BstX2I	RGATCY	4 cut(s)	22, 271, 478, 527
BstYI	RGATCY	4 cut(s)	22, 271, 478, 527
Bsu15I	ATCGAT	1 cut(s)	354
BsuI	GTATCC	1 cut(s)	218
BsuRI	GGCC	6 cut(s)	255, 293, 684, 768, 845, 1145
BsuTUI	ATCGAT	1 cut(s)	354
BtsCI	GGATG	2 cut(s)	663, 805
BveI	ACCTGC	1 cut(s)	177
Cac8I	GCNNGC	3 cut(s)	559, 715, 1165
CfoI	GCGC	3 cut(s)	190, 246, 563
Cfr10I	RCCGGY	1 cut(s)	364
Cfr13I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
ClaI	ATCGAT	1 cut(s)	354
CseI	GACGC	1 cut(s)	127
Csp6I	GTAC	2 cut(s)	300, 376
CviAII	CATG	3 cut(s)	558, 714, 1033
CviQI	GTAC	2 cut(s)	300, 376
DdeI	CTNAG	3 cut(s)	594, 774, 918
DpnI	GATC	9 cut(s)	24, 65, 161, 204, 273, 480, 529, 575, 827
DpnII	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
EaeI	YGGCCR	2 cut(s)	291, 766
Eam1104I	CTCTTC	1 cut(s)	474
EarI	CTCTTC	1 cut(s)	474
Eco130I	CCWWGG	1 cut(s)	653
Eco147I	AGGCCT	1 cut(s)	255
Eco24I	GRGCYC	1 cut(s)	180
Eco31I	GGTCTC	1 cut(s)	625
Eco47I	GGWCC	5 cut(s)	87, 130, 198, 549, 650
Eco57I	CTGAAG	1 cut(s)	703
Eco88I	CYCGRG	1 cut(s)	179
EcoO109I	RGGNCCY	2 cut(s)	87, 130
EcoRI	GAATTC	1 cut(s)	986
EcoRII	CCWGG	3 cut(s)	132, 194, 670
EcoT14I	CCWWGG	1 cut(s)	653
EcoT22I	ATGCAT	1 cut(s)	559
EcoT38I	GRGCYC	1 cut(s)	180
ErhI	CCWWGG	1 cut(s)	653
Esp3I	CGTCTC	1 cut(s)	110
FaeI	CATG	3 cut(s)	561, 717, 1036
FaqI	GGGAC	2 cut(s)	73, 1097
FatI	CATG	3 cut(s)	557, 713, 1032
FauNDI	CATATG	1 cut(s)	1123
FblI	GTMKAC	2 cut(s)	147, 789
Fnu4HI	GCNGC	4 cut(s)	122, 291, 899, 1052
FokI	GGATG	2 cut(s)	670, 812
FriOI	GRGCYC	1 cut(s)	180
Fsp4HI	GCNGC	4 cut(s)	122, 291, 899, 1052
FspBI	CTAG	3 cut(s)	26, 654, 840
FspI	TGCGCA	1 cut(s)	562
GlaI	GCGC	3 cut(s)	189, 245, 562
GluI	GCNGC	4 cut(s)	122, 291, 899, 1052
GsuI	CTGGAG	1 cut(s)	974
HaeIII	GGCC	6 cut(s)	255, 293, 684, 768, 845, 1145
HapII	CCGG	3 cut(s)	72, 305, 365
HgaI	GACGC	1 cut(s)	127
HhaI	GCGC	3 cut(s)	190, 246, 563
Hin1I	GRCGYC	3 cut(s)	5, 119, 144
Hin1II	CATG	3 cut(s)	561, 717, 1036
Hin6I	GCGC	3 cut(s)	188, 244, 561
HinP1I	GCGC	3 cut(s)	188, 244, 561
HindIII	AAGCTT	1 cut(s)	752
HinfI	GANTC	6 cut(s)	222, 239, 403, 509, 1063, 1094
HpaII	CCGG	3 cut(s)	72, 305, 365
HphI	GGTGA	3 cut(s)	382, 917, 1096
Hpy166II	GTNNAC	3 cut(s)	148, 621, 790
Hpy188I	TCNGA	6 cut(s)	68, 115, 202, 276, 385, 777
Hpy188III	TCNNGA	7 cut(s)	26, 140, 476, 577, 614, 983, 1138
Hpy8I	GTNNAC	3 cut(s)	148, 621, 790
HpyAV	CCTTC	2 cut(s)	245, 1135
HpyCH4III	ACNGT	1 cut(s)	892
HpyCH4IV	ACGT	1 cut(s)	144
HpyCH4V	TGCA	4 cut(s)	519, 557, 713, 1163
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 241, 252, 599, 681, 751, 809
HpyF3I	CTNAG	3 cut(s)	594, 774, 918
HpySE526I	ACGT	1 cut(s)	144
Hsp92I	GRCGYC	3 cut(s)	5, 119, 144
Hsp92II	CATG	3 cut(s)	561, 717, 1036
HspAI	GCGC	3 cut(s)	188, 244, 561
KflI	GGGWCCC	1 cut(s)	87
Kzo9I	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
LmnI	GCTCC	2 cut(s)	68, 386
Lsp1109I	GCAGC	2 cut(s)	910, 1063
LweI	GCATC	2 cut(s)	346, 790
MaeI	CTAG	3 cut(s)	26, 654, 840
MaeII	ACGT	1 cut(s)	144
MaeIII	GTNAC	3 cut(s)	851, 886, 1013
MalI	GATC	9 cut(s)	24, 65, 161, 204, 273, 480, 529, 575, 827
MbiI	CCGCTC	1 cut(s)	328
MboI	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
MboII	GAAGA	6 cut(s)	443, 461, 515, 722, 725, 850
MfeI	CAATTG	1 cut(s)	569
MflI	RGATCY	4 cut(s)	22, 271, 478, 527
MhlI	GDGCHC	1 cut(s)	180
MlsI	TGGCCA	1 cut(s)	768
MluCI	AATT	2 cut(s)	569, 986
MluNI	TGGCCA	1 cut(s)	768
MlyI	GAGTC	2 cut(s)	216, 233
MmeI	TCCRAC	2 cut(s)	93, 1040
Mox20I	TGGCCA	1 cut(s)	768
Mph1103I	ATGCAT	1 cut(s)	559
MscI	TGGCCA	1 cut(s)	768
MseI	TTAA	3 cut(s)	282, 498, 639
MslI	CAYNNNNRTG	1 cut(s)	263
Msp20I	TGGCCA	1 cut(s)	768
MspI	CCGG	3 cut(s)	72, 305, 365
MspR9I	CCNGG	3 cut(s)	134, 196, 672
MunI	CAATTG	1 cut(s)	569
MvaI	CCWGG	3 cut(s)	134, 196, 672
MvnI	CGCG	2 cut(s)	188, 244
MwoI	GCNNNNNNNGC	7 cut(s)	11, 241, 252, 599, 681, 751, 809
NdeI	CATATG	1 cut(s)	1123
NdeII	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
NlaIII	CATG	3 cut(s)	561, 717, 1036
NlaIV	GGNNCC	4 cut(s)	70, 88, 89, 480
NmeAIII	GCCGAG	1 cut(s)	1153
NmuCI	GTSAC	2 cut(s)	851, 1013
NsbI	TGCGCA	1 cut(s)	562
NsiI	ATGCAT	1 cut(s)	559
NspI	RCATGY	2 cut(s)	561, 717
PaeI	GCATGC	2 cut(s)	561, 717
PaqCI	CACCTGC	1 cut(s)	177
PceI	AGGCCT	1 cut(s)	255
PfeI	GAWTC	4 cut(s)	403, 509, 1063, 1094
PkrI	GCNGC	4 cut(s)	123, 292, 900, 1053
Ple19I	CGATCG	1 cut(s)	205
PleI	GAGTC	2 cut(s)	216, 233
PpsI	GAGTC	2 cut(s)	216, 233
PpuMI	RGGWCCY	2 cut(s)	87, 130
PshBI	ATTAAT	1 cut(s)	282
Psp5II	RGGWCCY	2 cut(s)	87, 130
Psp6I	CCWGG	3 cut(s)	132, 194, 670
PspGI	CCWGG	3 cut(s)	132, 194, 670
PspN4I	GGNNCC	4 cut(s)	70, 88, 89, 480
PspPI	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
PspPPI	RGGWCCY	2 cut(s)	87, 130
PsuI	RGATCY	4 cut(s)	22, 271, 478, 527
PvuI	CGATCG	1 cut(s)	205
RsaI	GTAC	2 cut(s)	301, 377
RsaNI	GTAC	2 cut(s)	300, 376
RseI	CAYNNNNRTG	1 cut(s)	263
SaqAI	TTAA	3 cut(s)	282, 498, 639
SatI	GCNGC	4 cut(s)	122, 291, 899, 1052
Sau3AI	GATC	9 cut(s)	22, 63, 159, 202, 271, 478, 527, 573, 825
Sau96I	GGNCC	7 cut(s)	87, 130, 198, 549, 650, 683, 843
ScaI	AGTACT	1 cut(s)	377
SchI	GAGTC	2 cut(s)	216, 233
ScrFI	CCNGG	3 cut(s)	134, 196, 672
SduI	GDGCHC	1 cut(s)	180
SfaNI	GCATC	2 cut(s)	346, 790
SfcI	CTRYAG	1 cut(s)	340
SinI	GGWCC	5 cut(s)	87, 130, 198, 549, 650
SmiMI	CAYNNNNRTG	1 cut(s)	263
SmlI	CTYRAG	4 cut(s)	173, 614, 749, 981
SmoI	CTYRAG	4 cut(s)	173, 614, 749, 981
SphI	GCATGC	2 cut(s)	561, 717
Sse9I	AATT	2 cut(s)	569, 986
SseBI	AGGCCT	1 cut(s)	255
SsiI	CCGC	4 cut(s)	122, 290, 326, 602
SspMI	CTAG	3 cut(s)	26, 654, 840
StuI	AGGCCT	1 cut(s)	255
StyD4I	CCNGG	3 cut(s)	132, 194, 670
StyI	CCWWGG	1 cut(s)	653
TaaI	ACNGT	1 cut(s)	892
TaiI	ACGT	1 cut(s)	147
TaqI	TCGA	5 cut(s)	249, 330, 354, 576, 828
TasI	AATT	2 cut(s)	569, 986
TatI	WGTACW	2 cut(s)	299, 375
TauI	GCSGC	2 cut(s)	124, 293
TfiI	GAWTC	4 cut(s)	403, 509, 1063, 1094
Tru1I	TTAA	3 cut(s)	282, 498, 639
Tru9I	TTAA	3 cut(s)	282, 498, 639
TseFI	GTSAC	2 cut(s)	851, 1013
TseI	GCWGC	2 cut(s)	898, 1051
Tsp45I	GTSAC	2 cut(s)	851, 1013
TspDTI	ATGAA	5 cut(s)	70, 340, 522, 655, 897
VpaK11BI	GGWCC	5 cut(s)	87, 130, 198, 549, 650
VspI	ATTAAT	1 cut(s)	282
XapI	RAATTY	1 cut(s)	986
XbaI	TCTAGA	1 cut(s)	25
XceI	RCATGY	2 cut(s)	561, 717
XmaJI	CCTAGG	1 cut(s)	653
XmiI	GTMKAC	2 cut(s)	147, 789
XspI	CTAG	3 cut(s)	26, 654, 840
ZraI	GACGTC	1 cut(s)	145
ZrmI	AGTACT	1 cut(s)	377
Zsp2I	ATGCAT	1 cut(s)	559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.