Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0003128

Physical Location & Seq

Genomic Coordinates Reverse (-)

53196364 .. 53199928

Gene Structure 3565 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG399600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 990 bp

ATGGCGTCGGCGGCGGGGAGAGTAGATCTGGACGGCAAGCCGATAAAGCCGATGACGATATGCATGATCGGAGCCGGAGGGTTCATTGGGTCCCACCTCTGCGAGAAGCTGATGGCGGAGACGCCGCACAAGGTTCTAGCCCTAGACGTGTACAATGACAAGATCAAGCACCTGCTGGAGCCATCGGACGGTCATCCTTGGTCCGATCGCATCCAGTTCCACCGCCTCAACATCAAGCACGACTCGCGCCTCGAAGGCCTCATCAAGACCTCAGATCTGACCATCAATCTCGCCGCGATCTGTACTCCGGCCGACTACAACACGCGCCCGCTGGACACTATCTACAGTAACTTCATCGATGCCTTGCCTGTGGTGAAGTACTGTTCTGAAAACAACAAGCGCTTGATTCACTTTTCGACTTGTGAAGTGTATGGGAAGACCATTGGAAGCTTTCTTCCTAAAGATAGCCCTCTTCGTCAGGATCCTGCATATTATCTGCTTAAAGAAGATGATTCCCCTTGCATCTTTGGGTCTATTGAGAAGCAGAGGTGGTCATATGCATGTGCAAAGCAATTGATCGAGAGGCTGATATATGCTGAGGGTGCAGAAAATGGTCTTGAGTTTACCATTGTAAGACCCTTTAACTGGATTGGACCTAGGATGGATTTCATTCCTGGGATTGATGGCCCAAGTGAGGGTGTTCCAAGAGTTCTAGCATGCTTTAGTAATAATCTTCTTCGTCGTGAACCACTCAAGCTTGTAGACGGTGGTGAATCCCAAAGAACTTTTGTTTATATAAAGGATGCTATTGATGCCGTTATGTTGATGATTGAAAATCCTGCTAGGGCCAATGGTCACATTTTCAATGTGGGCAACCCCAACAATGAAGTCACAGTTAGGCAGCTTGGTGAAATGATGACTGAGGTGATTATTTTACTTGTTAGAAAATCAAGCTGCAGTAGCAGTCCTGCATTGATTTTATTGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009987 GO:0016829 GO:0016830 GO:0016831 GO:0018130 GO:0019438 GO:0034641 GO:0034654 GO:0036094 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048037 GO:0048040 GO:0048046 GO:0050662 GO:0051287 GO:0055086 GO:0071704 GO:0097159 GO:1901135 GO:1901137 GO:1901265 GO:1901360 GO:1901362 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Epimerase

Protein Analysis

329

Amino Acids

36.71

Weight (kDa)

6.09

Isoelectric Point (pI)

44.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Epimerase	PF01370	20 - 291	2.1e-47	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	21 - 140	5e-06	Polysaccharide biosynthesis protein
3Beta_HSD	PF01073	22 - 146	4e-06	3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd	PF16363	24 - 304	5.1e-21	GDP-mannose 4,6 dehydratase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003128)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08200
fragaria_vesca	FvH4_3g30060 FvH4_3g30060 FvH4_3g30060 FvH4_3g30060
malus_domestica	MD03G1131500.v1.1 MD03G1131900.v1.1 MD03G1161900.v1.1 MD11G1154500.v1.1 MD11G1215900.v1.1 MD16G1216600.v1.1 MD16G1216800.v1.1
prunus_persica	Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1 Prupe.6G116900_v2.0.a1
pyrus_communis	pycom11g12600 pycom11g12610
rosa_chinensis	RchiOBHm_Chr5g0055821
rosa_laevigata	RLG00000002609 RLG00000035011
rosa_multiflora	Rmu_sc0003306.1_g000005 Rmu_sc0003500.1_g000032 Rmu_sc0003500.1_g000033 Rmu_sc0007802.1_g000009
rosa_roxburghii	Rroxscaffold_1G00024520 Rroxscaffold_1G00052150
rosa_rugosa	Rorug05G0295000
rosa_samantha	Rh5AG192800 Rh5AG365900 Rh5BG190400 Rh5BG376600 Rh5CG399600 Rh5DG192100 Rh5DG389500 Rh5DG389600 Rh7BG106200
rosa_wichuraiana	Rw5G034330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	180
Acc36I	ACCTGC	1 cut(s)	180
AccI	GTMKAC	1 cut(s)	762
AccII	CGCG	3 cut(s)	247, 296, 325
AciI	CCGC	7 cut(s)	11, 14, 116, 125, 223, 294, 329
AclWI	GGATC	2 cut(s)	476, 489
AcoI	YGGCCR	1 cut(s)	309
AcyI	GRCGYC	2 cut(s)	5, 122
AfaI	GTAC	3 cut(s)	152, 304, 380
AfeI	AGCGCT	1 cut(s)	401
AfiI	CCNNNNNNNGG	4 cut(s)	188, 645, 694, 695
AflIII	ACRYGT	1 cut(s)	147
AgsI	TTSAA	2 cut(s)	833, 865
AjiI	CACGTC	1 cut(s)	148
AjnI	CCWGG	1 cut(s)	673
AluBI	AGCT	5 cut(s)	109, 450, 757, 904, 954
AluI	AGCT	5 cut(s)	109, 450, 757, 904, 954
Alw26I	GTCTC	1 cut(s)	113
AlwI	GGATC	2 cut(s)	476, 489
Aor51HI	AGCGCT	1 cut(s)	401
AoxI	GGCC	4 cut(s)	256, 309, 685, 846
ApeKI	GCWGC	2 cut(s)	901, 954
AspA2I	CCTAGG	1 cut(s)	656
AspLEI	GCGC	3 cut(s)	249, 327, 402
AspS9I	GGNCC	5 cut(s)	90, 201, 653, 686, 846
AsuHPI	GGTGA	4 cut(s)	385, 782, 920, 937
AvaII	GGWCC	3 cut(s)	90, 201, 653
AvrII	CCTAGG	1 cut(s)	656
BamHI	GGATCC	1 cut(s)	481
BbsI	GAAGAC	1 cut(s)	443
BbvCI	CCTCAGC	1 cut(s)	597
BbvI	GCAGC	2 cut(s)	913, 941
BccI	CCATC	5 cut(s)	106, 190, 290, 655, 677
BceAI	ACGGC	2 cut(s)	49, 800
BciT130I	CCWGG	1 cut(s)	675
BcoDI	GTCTC	1 cut(s)	113
BfaI	CTAG	5 cut(s)	137, 143, 657, 713, 843
BfmI	CTRYAG	2 cut(s)	343, 955
BfoI	RGCGCY	1 cut(s)	403
BfuAI	ACCTGC	1 cut(s)	180
BglI	GCCNNNNNGGC	1 cut(s)	255
BglII	AGATCT	2 cut(s)	25, 274
BisI	GCNGC	5 cut(s)	12, 125, 294, 902, 955
BlnI	CCTAGG	1 cut(s)	656
BlsI	GCNGC	5 cut(s)	13, 126, 295, 903, 956
BmcAI	AGTACT	1 cut(s)	380
Bme1390I	CCNGG	1 cut(s)	675
Bme18I	GGWCC	3 cut(s)	90, 201, 653
BmgBI	CACGTC	1 cut(s)	148
BmgT120I	GGNCC	5 cut(s)	90, 201, 653, 686, 846
BmiI	GGNNCC	5 cut(s)	73, 91, 92, 180, 483
BmrFI	CCNGG	1 cut(s)	675
BmsI	GCATC	5 cut(s)	219, 349, 531, 793, 802
BpiI	GAAGAC	1 cut(s)	443
BpmI	CTGGAG	1 cut(s)	197
Bpu10I	CCTNAGC	1 cut(s)	597
BpuEI	CTTGAG	2 cut(s)	638, 737
Bsa29I	ATCGAT	1 cut(s)	357
BsaHI	GRCGYC	2 cut(s)	5, 122
BsaJI	CCNNGG	3 cut(s)	197, 656, 674
Bsc4I	CCNNNNNNNGG	4 cut(s)	188, 645, 694, 695
Bse1I	ACTGG	2 cut(s)	214, 650
BseBI	CCWGG	1 cut(s)	675
BseCI	ATCGAT	1 cut(s)	357
BseDI	CCNNGG	3 cut(s)	197, 656, 674
BseGI	GGATG	4 cut(s)	193, 210, 666, 808
BseLI	CCNNNNNNNGG	4 cut(s)	188, 645, 694, 695
BseMII	CTCAG	3 cut(s)	285, 588, 912
BseNI	ACTGG	2 cut(s)	214, 650
BseX3I	CGGCCG	1 cut(s)	309
BseXI	GCAGC	2 cut(s)	913, 941
BsgI	GTGCAG	1 cut(s)	624
Bsh1236I	CGCG	3 cut(s)	247, 296, 325
Bsh1285I	CGRYCG	2 cut(s)	208, 312
BshFI	GGCC	4 cut(s)	258, 311, 687, 848
BshVI	ATCGAT	1 cut(s)	357
BsiEI	CGRYCG	2 cut(s)	208, 312
BsiSI	CCGG	2 cut(s)	75, 308
BslFI	GGGAC	1 cut(s)	76
BslI	CCNNNNNNNGG	4 cut(s)	188, 645, 694, 695
BsmAI	GTCTC	1 cut(s)	113
BsmBI	CGTCTC	1 cut(s)	113
BsmFI	GGGAC	1 cut(s)	76
BsnI	GGCC	4 cut(s)	258, 311, 687, 848
Bsp1407I	TGTACA	1 cut(s)	150
Bsp143I	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
BspACI	CCGC	7 cut(s)	11, 14, 116, 125, 223, 294, 329
BspANI	GGCC	4 cut(s)	258, 311, 687, 848
BspCNI	CTCAG	3 cut(s)	284, 589, 913
BspDI	ATCGAT	1 cut(s)	357
BspFNI	CGCG	3 cut(s)	247, 296, 325
BspLI	GGNNCC	5 cut(s)	73, 91, 92, 180, 483
BspMAI	CTGCAG	1 cut(s)	959
BspMI	ACCTGC	1 cut(s)	180
BspPI	GGATC	2 cut(s)	476, 489
BsrGI	TGTACA	1 cut(s)	150
BsrI	ACTGG	2 cut(s)	214, 650
BssECI	CCNNGG	3 cut(s)	197, 656, 674
BssMI	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
BssNI	GRCGYC	2 cut(s)	5, 122
BssT1I	CCWWGG	2 cut(s)	197, 656
Bst2UI	CCWGG	1 cut(s)	675
Bst4CI	ACNGT	5 cut(s)	191, 347, 383, 767, 895
Bst6I	CTCTTC	1 cut(s)	477
BstACI	GRCGYC	2 cut(s)	5, 122
BstAUI	TGTACA	1 cut(s)	150
BstC8I	GCNNGC	3 cut(s)	38, 329, 718
BstDEI	CTNAG	3 cut(s)	271, 597, 921
BstF5I	GGATG	4 cut(s)	193, 210, 666, 808
BstFNI	CGCG	3 cut(s)	247, 296, 325
BstH2I	RGCGCY	1 cut(s)	403
BstHHI	GCGC	3 cut(s)	249, 327, 402
BstKTI	GATC	8 cut(s)	28, 69, 165, 208, 277, 300, 484, 579
BstMAI	GTCTC	1 cut(s)	113
BstMBI	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
BstMCI	CGRYCG	2 cut(s)	208, 312
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 46, 244, 255, 602, 812, 960
BstNI	CCWGG	1 cut(s)	675
BstNSI	RCATGY	2 cut(s)	564, 720
BstSCI	CCNGG	1 cut(s)	673
BstSFI	CTRYAG	2 cut(s)	343, 955
BstUI	CGCG	3 cut(s)	247, 296, 325
BstV1I	GCAGC	2 cut(s)	913, 941
BstV2I	GAAGAC	1 cut(s)	443
BstX2I	RGATCY	3 cut(s)	25, 274, 481
BstYI	RGATCY	3 cut(s)	25, 274, 481
BstZI	CGGCCG	1 cut(s)	309
Bsu15I	ATCGAT	1 cut(s)	357
BsuRI	GGCC	4 cut(s)	258, 311, 687, 848
BsuTUI	ATCGAT	1 cut(s)	357
BtrI	CACGTC	1 cut(s)	148
BtsCI	GGATG	4 cut(s)	193, 210, 666, 808
BveI	ACCTGC	1 cut(s)	180
Cac8I	GCNNGC	3 cut(s)	38, 329, 718
CfoI	GCGC	3 cut(s)	249, 327, 402
Cfr13I	GGNCC	5 cut(s)	90, 201, 653, 686, 846
ClaI	ATCGAT	1 cut(s)	357
CseI	GACGC	1 cut(s)	130
Csp6I	GTAC	3 cut(s)	151, 303, 379
CviAII	CATG	3 cut(s)	64, 561, 717
CviQI	GTAC	3 cut(s)	151, 303, 379
DdeI	CTNAG	3 cut(s)	271, 597, 921
DpnI	GATC	8 cut(s)	27, 68, 164, 207, 276, 299, 483, 578
DpnII	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
EaeI	YGGCCR	1 cut(s)	309
EagI	CGGCCG	1 cut(s)	309
Eam1104I	CTCTTC	1 cut(s)	477
EarI	CTCTTC	1 cut(s)	477
EciI	GGCGGA	1 cut(s)	131
EclXI	CGGCCG	1 cut(s)	309
Eco130I	CCWWGG	2 cut(s)	197, 656
Eco147I	AGGCCT	1 cut(s)	258
Eco47I	GGWCC	3 cut(s)	90, 201, 653
Eco47III	AGCGCT	1 cut(s)	401
Eco52I	CGGCCG	1 cut(s)	309
EcoO109I	RGGNCCY	1 cut(s)	90
EcoRII	CCWGG	1 cut(s)	673
EcoT14I	CCWWGG	2 cut(s)	197, 656
EcoT22I	ATGCAT	2 cut(s)	65, 562
ErhI	CCWWGG	2 cut(s)	197, 656
Esp3I	CGTCTC	1 cut(s)	113
FaeI	CATG	3 cut(s)	67, 564, 720
FaqI	GGGAC	1 cut(s)	76
FatI	CATG	3 cut(s)	63, 560, 716
FauI	CCCGC	2 cut(s)	7, 336
FauNDI	CATATG	1 cut(s)	556
FblI	GTMKAC	1 cut(s)	762
Fnu4HI	GCNGC	5 cut(s)	12, 125, 294, 902, 955
FokI	GGATG	4 cut(s)	180, 197, 673, 815
Fsp4HI	GCNGC	5 cut(s)	12, 125, 294, 902, 955
FspBI	CTAG	5 cut(s)	137, 143, 657, 713, 843
GlaI	GCGC	3 cut(s)	248, 326, 401
GluI	GCNGC	5 cut(s)	12, 125, 294, 902, 955
GsuI	CTGGAG	1 cut(s)	197
HaeII	RGCGCY	1 cut(s)	403
HaeIII	GGCC	4 cut(s)	258, 311, 687, 848
HapII	CCGG	2 cut(s)	75, 308
HgaI	GACGC	1 cut(s)	130
HhaI	GCGC	3 cut(s)	249, 327, 402
Hin1I	GRCGYC	2 cut(s)	5, 122
Hin1II	CATG	3 cut(s)	67, 564, 720
Hin6I	GCGC	3 cut(s)	247, 325, 400
HinP1I	GCGC	3 cut(s)	247, 325, 400
HindIII	AAGCTT	2 cut(s)	448, 755
HinfI	GANTC	4 cut(s)	242, 406, 512, 773
HpaII	CCGG	2 cut(s)	75, 308
HphI	GGTGA	4 cut(s)	385, 782, 920, 937
Hpy166II	GTNNAC	4 cut(s)	151, 624, 746, 763
Hpy188I	TCNGA	6 cut(s)	71, 187, 205, 274, 279, 388
Hpy188III	TCNNGA	6 cut(s)	29, 265, 479, 580, 617, 743
Hpy8I	GTNNAC	4 cut(s)	151, 624, 746, 763
Hpy99I	CGWCG	2 cut(s)	10, 744
HpyAV	CCTTC	1 cut(s)	248
HpyCH4III	ACNGT	5 cut(s)	191, 347, 383, 767, 895
HpyCH4IV	ACGT	1 cut(s)	147
HpyCH4V	TGCA	8 cut(s)	63, 488, 522, 560, 566, 605, 957, 971
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 46, 244, 255, 602, 812, 960
HpyF3I	CTNAG	3 cut(s)	271, 597, 921
HpySE526I	ACGT	1 cut(s)	147
Hsp92I	GRCGYC	2 cut(s)	5, 122
Hsp92II	CATG	3 cut(s)	67, 564, 720
HspAI	GCGC	3 cut(s)	247, 325, 400
KflI	GGGWCCC	1 cut(s)	90
Kzo9I	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
LmnI	GCTCC	2 cut(s)	71, 178
Lsp1109I	GCAGC	2 cut(s)	913, 941
LweI	GCATC	5 cut(s)	219, 349, 531, 793, 802
MaeI	CTAG	5 cut(s)	137, 143, 657, 713, 843
MaeII	ACGT	1 cut(s)	147
MaeIII	GTNAC	3 cut(s)	347, 854, 889
MalI	GATC	8 cut(s)	27, 68, 164, 207, 276, 299, 483, 578
MboI	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
MboII	GAAGA	6 cut(s)	446, 448, 464, 518, 725, 728
MfeI	CAATTG	1 cut(s)	572
MflI	RGATCY	3 cut(s)	25, 274, 481
MluCI	AATT	1 cut(s)	572
MlyI	GAGTC	1 cut(s)	236
Mph1103I	ATGCAT	2 cut(s)	65, 562
MseI	TTAA	2 cut(s)	501, 642
MslI	CAYNNNNRTG	1 cut(s)	559
MspA1I	CMGCKG	1 cut(s)	331
MspI	CCGG	2 cut(s)	75, 308
MspR9I	CCNGG	1 cut(s)	675
MunI	CAATTG	1 cut(s)	572
MvaI	CCWGG	1 cut(s)	675
MvnI	CGCG	3 cut(s)	247, 296, 325
MwoI	GCNNNNNNNGC	7 cut(s)	11, 46, 244, 255, 602, 812, 960
NdeI	CATATG	1 cut(s)	556
NdeII	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
NlaIII	CATG	3 cut(s)	67, 564, 720
NlaIV	GGNNCC	5 cut(s)	73, 91, 92, 180, 483
NmuCI	GTSAC	2 cut(s)	854, 889
NsiI	ATGCAT	2 cut(s)	65, 562
NspI	RCATGY	2 cut(s)	564, 720
PaeI	GCATGC	1 cut(s)	720
PaqCI	CACCTGC	1 cut(s)	180
PceI	AGGCCT	1 cut(s)	258
PfeI	GAWTC	3 cut(s)	406, 512, 773
PkrI	GCNGC	5 cut(s)	13, 126, 295, 903, 956
Ple19I	CGATCG	1 cut(s)	208
PleI	GAGTC	1 cut(s)	236
PpsI	GAGTC	1 cut(s)	236
PpuMI	RGGWCCY	1 cut(s)	90
Psp5II	RGGWCCY	1 cut(s)	90
Psp6I	CCWGG	1 cut(s)	673
PspGI	CCWGG	1 cut(s)	673
PspN4I	GGNNCC	5 cut(s)	73, 91, 92, 180, 483
PspPI	GGNCC	5 cut(s)	90, 201, 653, 686, 846
PspPPI	RGGWCCY	1 cut(s)	90
PstI	CTGCAG	1 cut(s)	959
PsuI	RGATCY	3 cut(s)	25, 274, 481
PvuI	CGATCG	1 cut(s)	208
RsaI	GTAC	3 cut(s)	152, 304, 380
RsaNI	GTAC	3 cut(s)	151, 303, 379
RseI	CAYNNNNRTG	1 cut(s)	559
SaqAI	TTAA	2 cut(s)	501, 642
SatI	GCNGC	5 cut(s)	12, 125, 294, 902, 955
Sau3AI	GATC	8 cut(s)	25, 66, 162, 205, 274, 297, 481, 576
Sau96I	GGNCC	5 cut(s)	90, 201, 653, 686, 846
ScaI	AGTACT	1 cut(s)	380
SchI	GAGTC	1 cut(s)	236
ScrFI	CCNGG	1 cut(s)	675
SfaNI	GCATC	5 cut(s)	219, 349, 531, 793, 802
SfcI	CTRYAG	2 cut(s)	343, 955
SinI	GGWCC	3 cut(s)	90, 201, 653
SmiMI	CAYNNNNRTG	1 cut(s)	559
SmlI	CTYRAG	2 cut(s)	617, 752
SmoI	CTYRAG	2 cut(s)	617, 752
SphI	GCATGC	1 cut(s)	720
Sse9I	AATT	1 cut(s)	572
SseBI	AGGCCT	1 cut(s)	258
SsiI	CCGC	7 cut(s)	11, 14, 116, 125, 223, 294, 329
SspMI	CTAG	5 cut(s)	137, 143, 657, 713, 843
StuI	AGGCCT	1 cut(s)	258
StyD4I	CCNGG	1 cut(s)	673
StyI	CCWWGG	2 cut(s)	197, 656
TaaI	ACNGT	5 cut(s)	191, 347, 383, 767, 895
TaiI	ACGT	1 cut(s)	150
TaqI	TCGA	4 cut(s)	252, 357, 416, 579
TasI	AATT	1 cut(s)	572
TatI	WGTACW	3 cut(s)	150, 302, 378
TauI	GCSGC	3 cut(s)	14, 127, 296
TfiI	GAWTC	3 cut(s)	406, 512, 773
Tru1I	TTAA	2 cut(s)	501, 642
Tru9I	TTAA	2 cut(s)	501, 642
TseFI	GTSAC	2 cut(s)	854, 889
TseI	GCWGC	2 cut(s)	901, 954
Tsp45I	GTSAC	2 cut(s)	854, 889
TspDTI	ATGAA	4 cut(s)	73, 343, 658, 900
VpaK11BI	GGWCC	3 cut(s)	90, 201, 653
XceI	RCATGY	2 cut(s)	564, 720
XmaJI	CCTAGG	1 cut(s)	656
XmiI	GTMKAC	1 cut(s)	762
XspI	CTAG	5 cut(s)	137, 143, 657, 713, 843
ZrmI	AGTACT	1 cut(s)	380
Zsp2I	ATGCAT	2 cut(s)	65, 562

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.