MD03G1224300.v1.1

General transcription factor 3C polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004376
Physical Location & Seq
Reverse (-)
30994104 .. 30997508
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1224300.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGGTGGGTGTCTACAGAAGCCCACTTGTAGCCTTTTCTCAAGTGGCCCGTTCAGTGTTGGGCTCGCAAATTTATCGCATCTCCAAACGCTGCGTATCGCTTCTTCTTTTTCTTCTTCTAATTCACCACGCTCGCTGCTGCTGCTGCTGGACATCTCAAAGCGTCAGCTCTTGCCCCACTGGTTCAAGACGTATGCTATGGAGTCAAAAGAAGAAGAAGAAGAAGAATATGTATTGCTCGATCTCGATTCCGTTTCCTCGCACTTTGAAATTCCACCAAACGCACCCTATTCTTTTTCCGGTCTAAACACGGAGCATCCAGTATTGACTATAGGCGACAAACTCAAGTTGATAGGAGAATATCAAGAAACGATTGGAACATGCCTTATATTCAAAGAAGAAGATGCTAGCCCTGGAGTTCATGAAGAAACGGGACCATCAGAAGCAAACCTTTTTGCAGGCAAATGCATAATGGATCCAAATCAATCTCCAAGCAAGCAAGTAAAACCAGTTACGAGCCTTCAAAAGATTTTAAAATTTAGATTGGCACCCAATCTCGAGTCTCATGTTCCACCTGAACAGTCTCGGGATCCGGTGCTACATACAGTCTCGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TFIIIC_sub6

Protein Analysis

206

Amino Acids

22.61

Weight (kDa)

5.35

Isoelectric Point (pI)

63.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIIC_sub6 PF10419 72 - 182 9e-19 TFIIIC subunit triple barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 1 cut(s) 13
AclWI GGATC 4 cut(s) 470, 483, 584, 597
AcsI RAATTY 3 cut(s) 70, 270, 536
AgsI TTSAA 4 cut(s) 187, 269, 394, 524
AjnI CCWGG 1 cut(s) 412
AluBI AGCT 1 cut(s) 169
AluI AGCT 1 cut(s) 169
Alw26I GTCTC 3 cut(s) 567, 588, 613
AlwI GGATC 4 cut(s) 470, 483, 584, 597
Ama87I CYCGRG 2 cut(s) 557, 585
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 5 cut(s) 91, 136, 139, 142, 145
ApoI RAATTY 3 cut(s) 70, 270, 536
AspS9I GGNCC 2 cut(s) 47, 434
AsuHPI GGTGA 1 cut(s) 117
AsuNHI GCTAGC 1 cut(s) 407
AvaI CYCGRG 2 cut(s) 557, 585
AvaII GGWCC 1 cut(s) 434
BamHI GGATCC 2 cut(s) 475, 589
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 66
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
BbvI GCAGC 5 cut(s) 78, 123, 126, 129, 132
BccI CCATC 1 cut(s) 445
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 1 cut(s) 414
BcoDI GTCTC 3 cut(s) 567, 588, 613
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 14, 330
BisI GCNGC 5 cut(s) 92, 137, 140, 143, 146
BlsI GCNGC 5 cut(s) 93, 138, 141, 144, 147
Bme1390I CCNGG 1 cut(s) 414
Bme18I GGWCC 1 cut(s) 434
BmeT110I CYCGRG 2 cut(s) 557, 585
BmgT120I GGNCC 2 cut(s) 47, 434
BmiI GGNNCC 4 cut(s) 435, 477, 549, 591
BmrFI CCNGG 1 cut(s) 414
BmsI GCATC 3 cut(s) 88, 325, 394
BmtI GCTAGC 1 cut(s) 411
BpmI CTGGAG 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 25, 329
BsaBI GATNNNNATC 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 412
BsaWI WCCGGW 2 cut(s) 299, 592
Bse1I ACTGG 3 cut(s) 185, 320, 509
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 1 cut(s) 316
BseJI GATNNNNATC 1 cut(s) 480
BseNI ACTGG 3 cut(s) 185, 320, 509
BseXI GCAGC 5 cut(s) 78, 123, 126, 129, 132
BshFI GGCC 1 cut(s) 48
BshNI GGYRCC 1 cut(s) 547
BsiHKCI CYCGRG 2 cut(s) 557, 585
BsiSI CCGG 2 cut(s) 300, 593
BslFI GGGAC 1 cut(s) 447
BsmAI GTCTC 3 cut(s) 567, 588, 613
BsmFI GGGAC 1 cut(s) 447
BsnI GGCC 1 cut(s) 48
BsoBI CYCGRG 2 cut(s) 557, 585
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 3 cut(s) 241, 475, 589
BspANI GGCC 1 cut(s) 48
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 4 cut(s) 435, 477, 549, 591
BspOI GCTAGC 1 cut(s) 411
BspPI GGATC 4 cut(s) 470, 483, 584, 597
BspT107I GGYRCC 1 cut(s) 547
BsrI ACTGG 3 cut(s) 185, 320, 509
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 3 cut(s) 241, 475, 589
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 2 cut(s) 582, 607
BstC8I GCNNGC 5 cut(s) 66, 134, 409, 460, 497
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 3 cut(s) 244, 478, 592
BstMAI GTCTC 3 cut(s) 567, 588, 613
BstMBI GATC 3 cut(s) 241, 475, 589
BstMWI GCNNNNNNNGC 2 cut(s) 142, 145
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 1 cut(s) 412
BstSFI CTRYAG 2 cut(s) 14, 330
BstV1I GCAGC 5 cut(s) 78, 123, 126, 129, 132
BstX2I RGATCY 2 cut(s) 475, 589
BstYI RGATCY 2 cut(s) 475, 589
BsuRI GGCC 1 cut(s) 48
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 2 cut(s) 61, 178
Cac8I GCNNGC 5 cut(s) 66, 134, 409, 460, 497
CciI TCATGA 1 cut(s) 421
Cfr13I GGNCC 2 cut(s) 47, 434
CseI GACGC 1 cut(s) 152
CviAII CATG 3 cut(s) 381, 422, 566
CviJI RGCY 7 cut(s) 22, 33, 48, 64, 169, 411, 519
CviKI_1 RGCY 7 cut(s) 22, 33, 48, 64, 169, 411, 519
DpnI GATC 3 cut(s) 243, 477, 591
DpnII GATC 3 cut(s) 241, 475, 589
DraI TTTAAA 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 434
Eco88I CYCGRG 2 cut(s) 557, 585
EcoRII CCWGG 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 470
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 3 cut(s) 384, 425, 569
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FaqI GGGAC 1 cut(s) 447
FatI CATG 3 cut(s) 380, 421, 565
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 5 cut(s) 92, 137, 140, 143, 146
FokI GGATG 1 cut(s) 303
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 5 cut(s) 92, 137, 140, 143, 146
FspBI CTAG 1 cut(s) 408
GluI GCNGC 5 cut(s) 92, 137, 140, 143, 146
GsuI CTGGAG 1 cut(s) 435
HaeIII GGCC 1 cut(s) 48
HapII CCGG 2 cut(s) 300, 593
HgaI GACGC 1 cut(s) 152
Hin1II CATG 3 cut(s) 384, 425, 569
HinfI GANTC 3 cut(s) 203, 248, 560
HpaII CCGG 2 cut(s) 300, 593
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 6 cut(s) 187, 245, 365, 422, 557, 587
Hpy8I GTNNAC 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 530
HpyCH4III ACNGT 2 cut(s) 582, 607
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 2 cut(s) 458, 468
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 145
HpySE526I ACGT 1 cut(s) 191
Hsp92II CATG 3 cut(s) 384, 425, 569
Kzo9I GATC 3 cut(s) 241, 475, 589
LmnI GCTCC 1 cut(s) 313
Lsp1109I GCAGC 5 cut(s) 78, 123, 126, 129, 132
LweI GCATC 3 cut(s) 88, 325, 394
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 1 cut(s) 511
MalI GATC 3 cut(s) 243, 477, 591
MboI GATC 3 cut(s) 241, 475, 589
MflI RGATCY 2 cut(s) 475, 589
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 4 cut(s) 70, 121, 270, 536
MlyI GAGTC 2 cut(s) 212, 569
MnlI CCTC 1 cut(s) 268
Mph1103I ATGCAT 1 cut(s) 470
MseI TTAA 1 cut(s) 533
MspI CCGG 2 cut(s) 300, 593
MspR9I CCNGG 1 cut(s) 414
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 142, 145
NdeII GATC 3 cut(s) 241, 475, 589
NheI GCTAGC 1 cut(s) 407
NlaIII CATG 3 cut(s) 384, 425, 569
NlaIV GGNNCC 4 cut(s) 435, 477, 549, 591
NsiI ATGCAT 1 cut(s) 470
NspI RCATGY 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 557
PagI TCATGA 1 cut(s) 421
PfeI GAWTC 1 cut(s) 248
PkrI GCNGC 5 cut(s) 93, 138, 141, 144, 147
PleI GAGTC 2 cut(s) 211, 568
PpsI GAGTC 2 cut(s) 211, 568
Psp6I CCWGG 1 cut(s) 412
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 4 cut(s) 435, 477, 549, 591
PspPI GGNCC 2 cut(s) 47, 434
PsuI RGATCY 2 cut(s) 475, 589
SaqAI TTAA 1 cut(s) 533
SatI GCNGC 5 cut(s) 92, 137, 140, 143, 146
Sau3AI GATC 3 cut(s) 241, 475, 589
Sau96I GGNCC 2 cut(s) 47, 434
SchI GAGTC 2 cut(s) 212, 569
ScrFI CCNGG 1 cut(s) 414
SduI GDGCHC 1 cut(s) 66
SetI ASST 4 cut(s) 171, 194, 453, 577
SfaNI GCATC 3 cut(s) 88, 325, 394
SfcI CTRYAG 2 cut(s) 14, 330
Sfr274I CTCGAG 1 cut(s) 557
SinI GGWCC 1 cut(s) 434
SlaI CTCGAG 1 cut(s) 557
SmlI CTYRAG 3 cut(s) 40, 344, 557
SmoI CTYRAG 3 cut(s) 40, 344, 557
Sse9I AATT 4 cut(s) 70, 121, 270, 536
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 412
TaaI ACNGT 2 cut(s) 582, 607
TaiI ACGT 1 cut(s) 194
TaqI TCGA 3 cut(s) 240, 246, 558
TasI AATT 4 cut(s) 70, 121, 270, 536
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 533
Tru9I TTAA 1 cut(s) 533
TscAI CASTG 2 cut(s) 61, 185
TseI GCWGC 5 cut(s) 91, 136, 139, 142, 145
TspDTI ATGAA 2 cut(s) 410, 438
TspGWI ACGGA 2 cut(s) 241, 326
TspRI CASTG 2 cut(s) 61, 185
VpaK11BI GGWCC 1 cut(s) 434
XapI RAATTY 3 cut(s) 70, 270, 536
XceI RCATGY 1 cut(s) 384
XhoI CTCGAG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 13
XspI CTAG 1 cut(s) 408
Zsp2I ATGCAT 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.