MD11G1243100.v1.1

General transcription factor 3C polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004376
Physical Location & Seq
Reverse (-)
35320447 .. 35324173
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1243100.v1.1.491

Sequence Viewer

Length: 402 bp
ATGAAACAAATTGGCGCCTTGCAGTTTGAAATTCCACCAAATGCACCGTATTCTTTTTCCGGTTTGAACACGGAGCATCCAGTATTGAGTATAGGTGACAAGCTCAAGTTGATAGGAGAATATCAAGAAACGGTTAGAACATGCCTTATATTCGAAGAAGAAGCCCTATTGCAGATGCTAGCGCAGCGGTTCATGAAGAAACGGGGCCATCGGAAGCAAGCAAACCCTTTTGCAGGCAAATGCATAATGGATCCAAATCAATCTCCAAGCAAGCAAGTGAAACCAGTTATGAGCCTTCAACGGATTTTAAAATTTCGATTGGCTCCCAATCTCGACTCTCATGATCCACCTGAACAGTCTCGGGATCCGGTGCTACATACAGTGCAATCAGTGGCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TFIIIC_sub6

Protein Analysis

134

Amino Acids

15.07

Weight (kDa)

8.98

Isoelectric Point (pI)

61.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIIC_sub6 PF10419 8 - 106 3.4e-10 TFIIIC subunit triple barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 14
AciI CCGC 1 cut(s) 187
AclWI GGATC 5 cut(s) 245, 258, 338, 359, 372
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 2 cut(s) 30, 311
AcyI GRCGYC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 233
AgsI TTSAA 3 cut(s) 29, 67, 299
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 5 cut(s) 245, 258, 338, 359, 372
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 2 cut(s) 205, 393
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 2 cut(s) 30, 311
AspLEI GCGC 2 cut(s) 17, 184
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 107
AsuII TTCGAA 1 cut(s) 153
AsuNHI GCTAGC 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 360
BamHI GGATCC 2 cut(s) 250, 364
BanI GGYRCC 1 cut(s) 14
BbvI GCAGC 1 cut(s) 196
BccI CCATC 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 179
BfoI RGCGCY 1 cut(s) 18
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 5 cut(s) 16, 206, 252, 324, 366
BmsI GCATC 2 cut(s) 85, 165
BmtI GCTAGC 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 89
BsaBI GATNNNNATC 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 15
BsaWI WCCGGW 2 cut(s) 59, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 80, 284
Bse8I GATNNNNATC 1 cut(s) 255
BseGI GGATG 1 cut(s) 76
BseJI GATNNNNATC 1 cut(s) 255
BseLI CCNNNNNNNGG 1 cut(s) 233
BseNI ACTGG 2 cut(s) 80, 284
BseXI GCAGC 1 cut(s) 196
BshFI GGCC 2 cut(s) 207, 395
BshNI GGYRCC 1 cut(s) 14
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 2 cut(s) 60, 368
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 363
BsnI GGCC 2 cut(s) 207, 395
BsoBI CYCGRG 1 cut(s) 360
Bsp119I TTCGAA 1 cut(s) 153
Bsp143I GATC 3 cut(s) 250, 343, 364
BspACI CCGC 1 cut(s) 187
BspANI GGCC 2 cut(s) 207, 395
BspHI TCATGA 2 cut(s) 192, 340
BspLI GGNNCC 5 cut(s) 16, 206, 252, 324, 366
BspOI GCTAGC 1 cut(s) 182
BspPI GGATC 5 cut(s) 245, 258, 338, 359, 372
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 14
BsrI ACTGG 2 cut(s) 80, 284
BssMI GATC 3 cut(s) 250, 343, 364
BssNI GRCGYC 1 cut(s) 15
Bst4CI ACNGT 4 cut(s) 48, 133, 357, 382
BstACI GRCGYC 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 4 cut(s) 180, 219, 235, 272
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 2 cut(s) 17, 184
BstKTI GATC 3 cut(s) 253, 346, 367
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 3 cut(s) 250, 343, 364
BstMWI GCNNNNNNNGC 1 cut(s) 184
BstNSI RCATGY 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 196
BstX2I RGATCY 2 cut(s) 250, 364
BstYI RGATCY 2 cut(s) 250, 364
BsuRI GGCC 2 cut(s) 207, 395
BtsCI GGATG 1 cut(s) 76
BtsIMutI CAGTG 2 cut(s) 387, 396
Cac8I GCNNGC 4 cut(s) 180, 219, 235, 272
CciI TCATGA 2 cut(s) 192, 340
CfoI GCGC 2 cut(s) 17, 184
Cfr13I GGNCC 1 cut(s) 205
CviAII CATG 3 cut(s) 141, 193, 341
CviJI RGCY 6 cut(s) 103, 164, 207, 294, 323, 395
CviKI_1 RGCY 6 cut(s) 103, 164, 207, 294, 323, 395
DinI GGCGCC 1 cut(s) 16
DpnI GATC 3 cut(s) 252, 345, 366
DpnII GATC 3 cut(s) 250, 343, 364
DraI TTTAAA 1 cut(s) 309
EaeI YGGCCR 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 360
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 245
EgeI GGCGCC 1 cut(s) 16
EheI GGCGCC 1 cut(s) 16
FaeI CATG 3 cut(s) 144, 196, 344
FaiI YATR 8 cut(s) 92, 142, 149, 194, 245, 290, 342, 378
FatI CATG 3 cut(s) 140, 192, 340
Fnu4HI GCNGC 1 cut(s) 185
FokI GGATG 1 cut(s) 63
Fsp4HI GCNGC 1 cut(s) 185
FspBI CTAG 1 cut(s) 179
GlaI GCGC 2 cut(s) 16, 183
GluI GCNGC 1 cut(s) 185
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 2 cut(s) 207, 395
HapII CCGG 2 cut(s) 60, 368
HhaI GCGC 2 cut(s) 17, 184
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 3 cut(s) 144, 196, 344
Hin6I GCGC 2 cut(s) 15, 182
HinP1I GCGC 2 cut(s) 15, 182
HinfI GANTC 1 cut(s) 335
HpaII CCGG 2 cut(s) 60, 368
HphI GGTGA 1 cut(s) 107
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 5 cut(s) 125, 193, 332, 341, 362
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 4 cut(s) 48, 133, 357, 382
HpyCH4V TGCA 6 cut(s) 22, 44, 172, 233, 243, 385
HpyF10VI GCNNNNNNNGC 1 cut(s) 184
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 3 cut(s) 144, 196, 344
HspAI GCGC 2 cut(s) 15, 182
KasI GGCGCC 1 cut(s) 14
Kzo9I GATC 3 cut(s) 250, 343, 364
LmnI GCTCC 2 cut(s) 73, 328
LpnPI CCDG 6 cut(s) 73, 93, 219, 297, 363, 381
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 85, 165
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 252, 345, 366
MboI GATC 3 cut(s) 250, 343, 364
MboII GAAGA 3 cut(s) 167, 170, 208
MflI RGATCY 2 cut(s) 250, 364
MluCI AATT 3 cut(s) 9, 30, 311
Mly113I GGCGCC 1 cut(s) 15
MlyI GAGTC 1 cut(s) 329
Mph1103I ATGCAT 1 cut(s) 245
MseI TTAA 1 cut(s) 308
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 2 cut(s) 60, 368
MwoI GCNNNNNNNGC 1 cut(s) 184
NarI GGCGCC 1 cut(s) 15
NdeII GATC 3 cut(s) 250, 343, 364
NheI GCTAGC 1 cut(s) 178
NlaIII CATG 3 cut(s) 144, 196, 344
NlaIV GGNNCC 5 cut(s) 16, 206, 252, 324, 366
NmuCI GTSAC 1 cut(s) 95
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 1 cut(s) 144
NspV TTCGAA 1 cut(s) 153
PagI TCATGA 2 cut(s) 192, 340
PkrI GCNGC 1 cut(s) 186
PleI GAGTC 1 cut(s) 329
PluTI GGCGCC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 329
PspN4I GGNNCC 5 cut(s) 16, 206, 252, 324, 366
PspPI GGNCC 1 cut(s) 205
PsuI RGATCY 2 cut(s) 250, 364
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 3 cut(s) 250, 343, 364
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 329
SetI ASST 3 cut(s) 97, 105, 352
SfaNI GCATC 2 cut(s) 85, 165
SfoI GGCGCC 1 cut(s) 16
SfuI TTCGAA 1 cut(s) 153
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 3 cut(s) 9, 30, 311
SsiI CCGC 1 cut(s) 187
SspDI GGCGCC 1 cut(s) 14
SspMI CTAG 1 cut(s) 179
TaaI ACNGT 4 cut(s) 48, 133, 357, 382
TaqI TCGA 3 cut(s) 153, 316, 333
TasI AATT 3 cut(s) 9, 30, 311
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 2 cut(s) 387, 396
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 3 cut(s) 17, 181, 209
TspGWI ACGGA 2 cut(s) 86, 316
TspRI CASTG 2 cut(s) 387, 396
XagI CCTNNNNNAGG 1 cut(s) 231
XapI RAATTY 2 cut(s) 30, 311
XceI RCATGY 1 cut(s) 144
XspI CTAG 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.