Rw5G021730

General transcription factor 3C polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004376
Physical Location & Seq
Forward (+)
29541416 .. 29543867
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021730.1

Sequence Viewer

Length: 435 bp
ATGGAGGGAGATACTGTGCCCAGTGAACATCAGGAACAAGAAGAAGAAGAAGAAGAAGAATATGTATTGCTTGATCTTGATTCTGTTTACTCCCAGCTTCAGATTCCACCAAAGGCACCATATGTTCTTTCAGGTTTGGATGCGATGCAACCGGTATTGATTCTAGATGGCAAATTTAAGTTGATCGGTCAGTGGGATGAGACAATTGGGACATGTCTTTTATTCAAAGAGGAAGTTGTCCCTGTTGTTCACGAAGAAACAGGACCATCAGAAGCAAACCTCAAGCAAGTAAAACCAGTTGGCCGCACTCATAGGATTCTAAAATTCAGATTGGCACCAGATTTTGACTCTCCTAATACTGCATCTGCACAGACTGAGCAAAAGGATAACTCGCATGTCAAGCTAATAGCTGAATGTTCCCTTTTTTTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TFIIIC_sub6

Protein Analysis

144

Amino Acids

16.2

Weight (kDa)

4.49

Isoelectric Point (pI)

58.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIIC_sub6 PF10419 16 - 111 2.2e-16 TFIIIC subunit triple barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 115, 334
AciI CCGC 1 cut(s) 304
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 2 cut(s) 173, 323
AcuI CTGAAG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 212
AgeI ACCGGT 1 cut(s) 151
AgsI TTSAA 1 cut(s) 226
AluBI AGCT 3 cut(s) 97, 403, 410
AluI AGCT 3 cut(s) 97, 403, 410
Alw26I GTCTC 1 cut(s) 194
AoxI GGCC 1 cut(s) 301
ApoI RAATTY 2 cut(s) 173, 323
AsiGI ACCGGT 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 263
AvaII GGWCC 1 cut(s) 263
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 2 cut(s) 115, 334
BccI CCATC 2 cut(s) 161, 274
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 1 cut(s) 164
BisI GCNGC 1 cut(s) 304
BlsI GCNGC 1 cut(s) 305
Bme18I GGWCC 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 117, 336
BmrI ACTGGG 1 cut(s) 15
BmsI GCATC 3 cut(s) 130, 135, 371
BmuI ACTGGG 1 cut(s) 15
BpuEI CTTGAG 1 cut(s) 266
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 2 cut(s) 21, 296
BseGI GGATG 2 cut(s) 145, 202
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 2 cut(s) 21, 296
BseSI GKGCMC 1 cut(s) 21
BseYI CCCAGC 1 cut(s) 93
BsgI GTGCAG 1 cut(s) 351
BshFI GGCC 1 cut(s) 303
BshNI GGYRCC 2 cut(s) 115, 334
BshTI ACCGGT 1 cut(s) 151
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 223, 224
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 2 cut(s) 223, 224
BsnI GGCC 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 21
Bsp143I GATC 2 cut(s) 73, 183
BspACI CCGC 1 cut(s) 304
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 1 cut(s) 367
BspLI GGNNCC 2 cut(s) 117, 336
BspT107I GGYRCC 2 cut(s) 115, 334
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 2 cut(s) 21, 296
BssAI RCCGGY 1 cut(s) 151
BssMI GATC 2 cut(s) 73, 183
Bst4CI ACNGT 1 cut(s) 16
BstDEI CTNAG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 145, 202
BstKTI GATC 2 cut(s) 76, 186
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 2 cut(s) 73, 183
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstNSI RCATGY 2 cut(s) 216, 398
BstSLI GKGCMC 1 cut(s) 21
BsuRI GGCC 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 158
BtsCI GGATG 2 cut(s) 145, 202
BtsIMutI CAGTG 2 cut(s) 28, 197
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 263
CspAI ACCGGT 1 cut(s) 151
CviAII CATG 2 cut(s) 213, 395
CviJI RGCY 4 cut(s) 97, 303, 403, 410
CviKI_1 RGCY 4 cut(s) 97, 303, 403, 410
DdeI CTNAG 1 cut(s) 375
DpnI GATC 2 cut(s) 75, 185
DpnII GATC 2 cut(s) 73, 183
EaeI YGGCCR 1 cut(s) 301
Eco47I GGWCC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 83
FaeI CATG 2 cut(s) 216, 398
FaiI YATR 7 cut(s) 63, 121, 123, 214, 312, 396, 433
FaqI GGGAC 2 cut(s) 223, 224
FatI CATG 2 cut(s) 212, 394
FauNDI CATATG 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 304
FokI GGATG 2 cut(s) 152, 209
Fsp4HI GCNGC 1 cut(s) 304
FspBI CTAG 1 cut(s) 164
GluI GCNGC 1 cut(s) 304
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 1 cut(s) 303
HapII CCGG 1 cut(s) 152
Hin1II CATG 2 cut(s) 216, 398
HinfI GANTC 5 cut(s) 80, 103, 160, 316, 347
HpaII CCGG 1 cut(s) 152
Hpy166II GTNNAC 3 cut(s) 26, 88, 250
Hpy188I TCNGA 3 cut(s) 102, 271, 329
Hpy188III TCNNGA 4 cut(s) 32, 77, 164, 251
Hpy8I GTNNAC 3 cut(s) 26, 88, 250
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4V TGCA 3 cut(s) 148, 362, 368
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 1 cut(s) 375
Hsp92II CATG 2 cut(s) 216, 398
Kzo9I GATC 2 cut(s) 73, 183
LpnPI CCDG 9 cut(s) 17, 34, 107, 117, 165, 246, 255, 309, 351
LweI GCATC 3 cut(s) 130, 135, 371
MaeI CTAG 1 cut(s) 164
MalI GATC 2 cut(s) 75, 185
MboI GATC 2 cut(s) 73, 183
MboII GAAGA 7 cut(s) 53, 56, 59, 62, 65, 68, 266
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 3 cut(s) 173, 204, 323
MlyI GAGTC 1 cut(s) 341
MnlI CCTC 2 cut(s) 223, 290
MseI TTAA 1 cut(s) 177
MspI CCGG 1 cut(s) 152
MunI CAATTG 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeI CATATG 1 cut(s) 121
NdeII GATC 2 cut(s) 73, 183
NlaIII CATG 2 cut(s) 216, 398
NlaIV GGNNCC 2 cut(s) 117, 336
NspI RCATGY 2 cut(s) 216, 398
PciI ACATGT 1 cut(s) 212
PfeI GAWTC 4 cut(s) 80, 103, 160, 316
PinAI ACCGGT 1 cut(s) 151
PkrI GCNGC 1 cut(s) 305
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
PscI ACATGT 1 cut(s) 212
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 2 cut(s) 117, 336
PspPI GGNCC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 1 cut(s) 304
Sau3AI GATC 2 cut(s) 73, 183
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 1 cut(s) 341
SduI GDGCHC 1 cut(s) 21
SetI ASST 5 cut(s) 99, 136, 282, 405, 412
SfaNI GCATC 3 cut(s) 130, 135, 371
SinI GGWCC 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 3 cut(s) 173, 204, 323
SsiI CCGC 1 cut(s) 304
SspMI CTAG 1 cut(s) 164
TaaI ACNGT 1 cut(s) 16
TaqII GACCGA 1 cut(s) 176
TasI AATT 3 cut(s) 173, 204, 323
TauI GCSGC 1 cut(s) 306
TfiI GAWTC 4 cut(s) 80, 103, 160, 316
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 2 cut(s) 28, 197
TspDTI ATGAA 1 cut(s) 420
TspRI CASTG 2 cut(s) 28, 197
VpaK11BI GGWCC 1 cut(s) 263
XapI RAATTY 2 cut(s) 173, 323
XbaI TCTAGA 1 cut(s) 163
XceI RCATGY 2 cut(s) 216, 398
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.