MD04G1047300.v1.1

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004130
Physical Location & Seq
Forward (+)
5512306 .. 5533698
21393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1047300.v1.1.491

Sequence Viewer

Length: 5601 bp
ATGTTTGGCAATGTCAATGACTCTGGTGGGCTTGATGCTGATTACCTTGATGAGGATGCAAAGGAACATCTTGCTGCACTTGCTGACAAGCTGGGTCCATCATTAACAGGCATAAATCTGTCAGTAAAATCACCACAAACATCAACTGATGCTGTTGAAGAAGATTATGAAGAGAAGGCTGAAAATGCAGTTAATTATTTTGATATTGATGAGGATTTTGAGGGACCAGAGATTCAAGCTGCTACTGAGGAGGACCATTTGTTGCCAAGAAAGGAATATTTTTCTGCTCAAGGTTCATTTGCTACTTTGGAGCCCACAAATTCTATGTTTGATGATGAAGATTATGATGAAGAAATTGGACAGGAACATGAGGTGGTTGAAAGCAACATAGATGTTCAAACTATCTCTTTACCAGGGGTTTCTACTGGAGAAAAATATTCTGAGGATGATTGTCAAATTGGGGGTCTGGACTCTGGAAATTTGGCTGCTCGTGCAGAAGAGTTTCAGGAAGTGCTTACAGATAAAAGTGCCACACCGCTGCCAGTTCTATGCATAGAAGATGGAAAGGTGATATTACGGTTCTCGGAAATCTTTGGTATTCATGTGCCCTTGAAGAAGGCGGAGAAAAGAGATCATAGGTACTCTGTTCCTAAAGATAGGTACAAATCCATGGATGTTTCTGAGATTGTTGAAGAGGATGAAGAGTCATTCTTAAAAGGATCTGGTCAAGGATTTCAATCTTTGAAACAGGCAGATGCACTCAAACATGACATTTTTTCAGCACTCTATGATGAAGACTCGGACTTCGCAAAGTTTCGTGTTCTAAAAGGGGCAAATTCAGTGGATCTGCTGGATGACAGGGCAATAAAGGACTCTTGTCTCATTGCGGAACGAATGAAAGAGAATCAAATAGTTGACATTTCTGTGGAAAGGCAATCACCTTTGTGTTCAAAATTTTATCCTCTTGATCAACAAGACTGGGAAGAGGGAATTGTTTGGGGAAATTCTCCTATAGCAAGTGACAATTCTGTTGAGAGTTGTGAAATTTCGGGACCTGACGAAGCTTCATTTAATAGTGAAACAGAACCTTATAGTGGGACACAAAATATTCAGTTAAAGCCCCAAAAAGAACCTGATGAGAAGGATCACGCTGTTATGCTGCGTAGCTCATGTCTATTGGAGCCTTTTGGCTCAAGAAATTCATCAGAATTTTCATCTCTTCCTTTCTCGGAAAGCAGACGCCATCCCCAACTTTTAAGGCTAGAATCTAGATTTGAAGTGGATGACCATGCTGATGGTGCAATGGAGAGTGTCAGTAAGAAGCTTCATCAGAGTGACGCTGTGAGGCAATTTAGCAAACTCACTTCACAGAACAGAGACATGCTGGAAGGATCTTGGTTAGACCAGATAATATGGGACCCAGATATGCCTACTGGGAAGTCAAAGCTTATCCTTGATCTTCAAGATGAGCAAATGCTTTTTGAGATTTTGGATAACAAGGAAAGTGAACATCTCAGGCTTCATTCCGGGGCCATGATTGTGACTCGCCCAATAAAGTCAAGTAATGGGGATTCTTTTGAGTTTCCAGGTCATGGAGGTCAATCTGGGTGGCGATATGTTTCTAATGATAAACACTATTCAAATAGGAAAACTTCTCAGCAACTGAAATCAAATTCTAAAAAACGCACAGCGCAGGGCATAAAGATTTATCATTCACAACCTGCACTGATGTTACAGACAATGAAACTGAGGTTAAGCAATAAGGATGTGGCAAATTTTCACCGACCAAAATCTTTATGGTATCCTCATGACAATGAAGTGGCTGTAAAAGAACGAGGAAAGCTCCCCACGCAAGGACCAATGAAAATTATAGTTAAAAGCTTGGGTGGCAAAGGAAGTAAGCTTCATGTGGATGCTGAGGAAACAATATCTTCTGTTAAAGCAAAAGCTTCAAAGAAGTTAGATTTTAAGCCATCCGAAACAGTGAAGATGTTCTATTTAGGGAAGGAACTTGAAGATGATAAATCTCTTACTGTCCAGAATGTTCAACCAAATTCTTTGCTTCATCTTGTTCGCACAAAAATATATTTGTTGCCAAGAGCACAAAAGATTCCTGGTGAAAATAAATCTTTGCGGCCTCCTGGAGCATTTAAGAAGAAATCTGATCTTTCTGTGAAAGATGGTCATGTATTCCTAATGGAGTACTGTGAAGAAAGACCTTTACTTTTGAGCAATGCTGGTATGGGTGCAAGATTATGTACTTATTACCAAAAATCAGCCCCAGATGATCAAACTGCTTCTTTGTTACGCAATGATAACCACAGTTTGGGACATGTCATTTCACTAAATCCAGCTGATAAATCACCTTTTCTTGGAGATACAAAAGCTGGTTGTAGTCAGTCATCTCTTGAAACAAATATGTATAGAGCACCTGTATTTTCTCATAAGGTGCCCTCAACTGATTATTTGTTAGTTCGTTCTGCAAAGGGGAAGCTTTCAATTAGACGTATTGACCGCCTAAATGTTGTTGGGCAACAGGAACCACTCATGGATGTAATGTCTCCTGGAACTAAGAATCTTCAAAATTATATGATCAATAGGCTTCTTGTCTACATCTGCCGAGAGTTCCGTGCTGCCGAAAAGCGCCATTTACTTCCTTGTATCCGCGCAGATGAGTTACCTTCACAATTTCCTTATCTATCTGAACCCTTTCTTCGGAAGAAGCTGAAGGAACATGCAAATTTCCAGAGAGCATCAAATGGACAATGTATGTGGGTTAAGAAACGCAACTTCCGGATTTTGTCGGAGGATGAATTAAGGAATTTGGTGAAACCAGAAGAGGTGTGTGCCTATGAAAGCATGCAAGCTGGTCTTTACCGGCTCAAACATTTGGGAATTACTGAAACACATCCGTCTGCCATATCTTCTGCAATGAGTCGTCTCCCTGATGAAGCAATTACTCTGGCTGCTGCATCTCACATTGAAAGAGAACTGCAGATCACTCCATGGAACTTGAGTAGCAACTTTGTAACCTGTACACAGGGAAAAGAAAATATTGAGCGTTTGGAAATATCTGGTGTTGGTGATCCATCTGGCCGGGGCCTAGGTTTCAGTTATGTTCGTGCTGCTCCAAAACCATCAATGTCAAGTGCAGTCGTGAAGAAAAAATCTGCTGCTGGTCGAGGAGGTTCCACTGTTACTGGAACAGATGCTGATCTACGCAGATTAAGCATGGAGGCTGCACGAGAGGTTCTTCTTAAATTTGGCGTTTCTGATGAGCTGATTGCAAGGCAGACTAGATGGCATCGTATTGCGATGATACGCAAGCTTTCAAGTGAGCAAGCTGCATCTGGGGTTAAAGTTGATGCAAACACTATCAGTAAATATGCACGTGGCCAGCGTATGTCCTTCCTTCAGCTGCAGCAGCAGAACCGGGAGAAGTGCCAAGAAATTTGGGATCGGCAAGTGCAGAGTCTTTCAGCTATAGATGGGGAAGAAAATGAGAGTGACTCTGAAGGAAATAATAGTGATCTAGATTCCTTTGCTGGGGATTTAGAAAATCTGCTTGATGCAGAAGAATGTGAAGAAGGGCTAGGTGGTGTTCATGAATCCAATCATGATAAATCAGATGGTGTTAAAGGGCTTAAAATGAGAAGGCGCCCTTCCTTGGCTCAGGCAGAAGAGGAAATTGAAGATGAAGCAGCTGAAGCAGCTGAGTTATGCAGGTTGCTCATGGATGATGATGAAACTGAGAGGAAAAAGAAGAAGAAAACAAGAGTTTTGGGGGAGGAAGTTGGGCTGGCTCCTGGCATGCGAACAAGTTATATCGTAGAAAATGCAGATAGGGGGAAAAAAATAATTGCTGCTGTACAACCTGATGGATTCTATACTCCAAAAGATAATTCAGTCGGAGATGCGAAAGTGGTGGAAAATTTTCTTAAAAGAAACAAGACTGGAAAACTAAAAGGAATGAAAAAGAATGATACTACACATACGGGTCTGATGAACAAGAAACTGAAAATATCAGGAGATGGTGTCAAGAGTACTTTTAAGGAGAAGAAATCAGCACGAGAGAAATTTATCTGTGGAGCATGTGGTCAGCTTGGACACATGAGGACAAACAAGCACTGCCCCAAGTACGGATATGGAGAAGATCAGGAAACACAGAATGATACTCCGGACCCTGATAAATCATCTGGAAAGACAACGGCTCTGAATTCATCTAGTCAGTCTCAACAGAAAACTACTACAAAGAAGCTGGTACCTAAAAGTGCAACTAAAATTGCCGTGGTTGAAGCTTCGGAGGTTGAAAACCTTGGGCTGAGCACAAAGGTTCTTCCATTGAAATTCAAATGTGGGTCAACTGAAAAGCTTCCTGATAAACAGCCTCTTGGAGAGACAGAGAGTTCTGAGCGGGTCACATCTGATCCCGAAAATGGGAAGCCCACTATGAAGGTGCACAAAATAATAATTTCGAACAAGATGAAACCTGAAAATGTACCTGTTGAACCTCAGAAGCCCCCTATTGTGATGCGACCTCTGACAGATACAGATAGGGGTTATGTCGAATCACAAAAGCAAACTATTGTTATACGGCCACCAGCAAATACTGATAGAGACCAGGGGGAGTCTCAAAAGCTCTCAGTTGCTAAACGGTCGTCAATGGAGGCAAAGAGAGAGCAACATCACAAGAAAATTATAATTAAACGTCCAAAAGAAATTATTGATATAGATCAGATCAGTCAGGATGGGGGAACTCCTGTTGAACACAGGAAAACTAAAAGAATTGTTGAATTGACAAGTTCTGAAAAGCACAGGAAGCAGGAGAACGTGTATTTGGCTAAGGAAGCAGCAAACAAGAAAGCTAGAGATGAAAGGAGATTACGAGAGGAGCAAGAGAAACGGATAAATGAAGACAGATTGAGAGAAGAGAGGGCCAGGAGGCTTTATGAAGAGGAAATGAGGATGATAGAGGAGCGAGAAAGAATAGCAGAGCTTAGAAGATATGAAGCATTAATCAGACAAGAGAGGGAGGAAGAAGAACGCCAGAAAGCAAAGAACAAAATGAAGAAGAAAAGGTCTGAGATAAGAGATGATTATTTAGAGGACTCACGTGCACGAAGATTAGACAAAAGGATGCCAGAAAGAGATCGGGGTGCAAAAAGGAGGCCAGTCGTTGAATTGGGAAGGCATGGTGGAGAATCTACCCCAGCAACAAAGCGCCGTAGAGGGGGTGAGGTTGGTCTGGCAAACATCTTAGAGCGTATTGTAGAAACCCTCAAAGATCGAATTGAAGTATCATATCTTTTTCTAAAACCTGTATCCAAAAAGGAAGCTCCTGATTACTTGGACATCATAGAGCGCCCCATGGATCTGTCTACAATCAAGGAGAAGGTGAGGAAAATGGAGTACAAGAGCAGGGAGCAATTCAGGCACGACGTGTGGCAGATTACTTATAATGCCCACAGATATAACGATGGACGCAACCCAGGCATACCTCCCCTTGCAGATCAGCTCTTAGAGCTTTGTGACTATATGTTGGTTGAGAACGACGAAAGCTTGACTGAAGCCGAAGCGGGCATTGAATATGTAGATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0000988 GO:0000989 GO:0001076 GO:0001129 GO:0001132 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0006139 GO:0006325 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016591 GO:0016740 GO:0016746 GO:0016747 GO:0017025 GO:0018130 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034212 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0055029 GO:0060255 GO:0061695 GO:0061733 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
Bromodomain DUF3591 TBP-binding ubiquitin zf-CCHC_6

Protein Analysis

1867

Amino Acids

210.86

Weight (kDa)

6.29

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBP-binding PF09247 1 - 28 2.1e-06 TATA box-binding protein binding
DUF3591 PF12157 540 - 1105 1.5e-110 Protein of unknown function (DUF3591)
ubiquitin PF00240 623 - 693 2.5e-10 Ubiquitin family
Bromodomain PF00439 1754 - 1828 3e-18 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4700, 5457
Acc36I ACCTGC 2 cut(s) 1729, 3714
Acc65I GGTACC 1 cut(s) 4261
AccB1I GGYRCC 3 cut(s) 2449, 3657, 4261
AccB7I CCANNNNNTGG 2 cut(s) 1592, 2326
AccBSI CCGCTC 1 cut(s) 4414
AccI GTMKAC 2 cut(s) 2610, 5378
AccII CGCG 1 cut(s) 2667
AccIII TCCGGA 2 cut(s) 2793, 4177
AciI CCGC 8 cut(s) 536, 620, 887, 2134, 2515, 2665, 4414, 5576
AclWI GGATC 8 cut(s) 727, 852, 1152, 1399, 3080, 3465, 4421, 5379
AcoI YGGCCR 3 cut(s) 3094, 3394, 4595
AcuI CTGAAG 5 cut(s) 2747, 3398, 3534, 3726, 5586
AcvI CACGTG 2 cut(s) 3392, 5114
AcyI GRCGYC 2 cut(s) 1240, 3658
AdeI CACNNNGTG 1 cut(s) 5440
AflIII ACRYGT 3 cut(s) 2332, 4830, 5439
AjiI CACGTC 1 cut(s) 5440
AjnI CCWGG 9 cut(s) 412, 1585, 2113, 2140, 2563, 3805, 4620, 4937, 5488
AjuI GAANNNNNNNTTGG 4 cut(s) 3886, 3918, 4820, 4852
AleI CACNNNNGTG 1 cut(s) 943
Alw21I GWGCWC 5 cut(s) 2104, 2431, 4328, 4461, 5119
Alw26I GTCTC 8 cut(s) 884, 1371, 2565, 2945, 4236, 4391, 4611, 4635
Alw44I GTGCAC 2 cut(s) 4457, 5115
AlwI GGATC 8 cut(s) 727, 852, 1152, 1399, 3080, 3465, 4421, 5379
AlwNI CAGNNNCTG 2 cut(s) 1663, 3212
Aor13HI TCCGGA 2 cut(s) 2793, 4177
AoxI GGCC 8 cut(s) 1530, 2135, 3094, 3100, 3394, 4595, 4935, 5168
ApaLI GTGCAC 2 cut(s) 4457, 5115
ArsI GACNNNNNNTTYG 4 cut(s) 448, 480, 788, 820
AseI ATTAAT 1 cut(s) 5015
Asp700I GAANNNNTTC 5 cut(s) 501, 1991, 2709, 3933, 4371
Asp718I GGTACC 1 cut(s) 4261
AspA2I CCTAGG 1 cut(s) 3103
AspLEI GCGC 6 cut(s) 1693, 2646, 2669, 3660, 5223, 5364
AsuC2I CCSGG 3 cut(s) 1528, 3098, 3434
AsuII TTCGAA 1 cut(s) 4475
AvaII GGWCC 7 cut(s) 95, 224, 253, 1052, 1417, 1856, 4180
AvrII CCTAGG 1 cut(s) 3103
BaeGI GKGCMC 4 cut(s) 609, 2454, 4461, 5119
BalI TGGCCA 1 cut(s) 3396
BanI GGYRCC 3 cut(s) 2449, 3657, 4261
BanII GRGCYC 1 cut(s) 315
BauI CACGAG 3 cut(s) 489, 3243, 4068
BbrPI CACGTG 2 cut(s) 3392, 5114
BbsI GAAGAC 2 cut(s) 801, 4920
Bbv12I GWGCWC 5 cut(s) 2104, 2431, 4328, 4461, 5119
BbvCI CCTCAGC 1 cut(s) 1917
BceAI ACGGC 4 cut(s) 4224, 4271, 4610, 5208
BciT130I CCWGG 9 cut(s) 414, 1587, 2115, 2142, 2565, 3807, 4622, 4939, 5490
BciVI GTATCC 3 cut(s) 1812, 2672, 5332
BclI TGATCA 3 cut(s) 967, 2287, 2592
BcnI CCSGG 3 cut(s) 1528, 3098, 3434
BcoDI GTCTC 8 cut(s) 884, 1371, 2565, 2945, 4236, 4391, 4611, 4635
BfaI CTAG 9 cut(s) 1262, 1269, 3104, 3297, 3533, 3593, 4224, 4866, 5599
BfmI CTRYAG 4 cut(s) 1011, 2993, 3419, 3483
BfoI RGCGCY 4 cut(s) 2647, 3661, 5224, 5365
BfuAI ACCTGC 2 cut(s) 1729, 3714
BfuI GTATCC 3 cut(s) 1812, 2672, 5332
BlnI CCTAGG 1 cut(s) 3103
BlpI GCTNAGC 1 cut(s) 4322
BmcAI AGTACT 2 cut(s) 2204, 4045
Bme18I GGWCC 7 cut(s) 95, 224, 253, 1052, 1417, 1856, 4180
BmgBI CACGTC 1 cut(s) 5440
BmrI ACTGGG 2 cut(s) 988, 1443
BmuI ACTGGG 2 cut(s) 988, 1443
BoxI GACNNNNGTC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 801, 4920
BplI GAGNNNNNCTC 4 cut(s) 1827, 1859, 3494, 3526
BpmI CTGGAG 2 cut(s) 447, 2163
Bpu10I CCTNAGC 3 cut(s) 1917, 3672, 4842
Bpu1102I GCTNAGC 1 cut(s) 4322
Bpu14I TTCGAA 1 cut(s) 4475
BpuEI CTTGAG 3 cut(s) 273, 1177, 3034
BpuMI CCSGG 3 cut(s) 1528, 3098, 3434
BsaAI YACGTR 2 cut(s) 3392, 5114
BsaBI GATNNNNATC 3 cut(s) 736, 3213, 4731
BsaHI GRCGYC 2 cut(s) 1240, 3658
BsaI GGTCTC 1 cut(s) 4611
BsaWI WCCGGW 2 cut(s) 2793, 4177
BsaXI ACNNNNNCTCC 4 cut(s) 4020, 4050, 4817, 4847
Bse118I RCCGGY 1 cut(s) 2877
Bse1I ACTGG 7 cut(s) 430, 542, 983, 1438, 3205, 3958, 5171
Bse3DI GCAATG 6 cut(s) 16, 882, 1308, 2239, 2317, 2937
Bse8I GATNNNNATC 3 cut(s) 736, 3213, 4731
BseAI TCCGGA 2 cut(s) 2793, 4177
BseBI CCWGG 9 cut(s) 414, 1587, 2115, 2142, 2565, 3807, 4622, 4939, 5490
BseJI GATNNNNATC 3 cut(s) 736, 3213, 4731
BseMI GCAATG 6 cut(s) 16, 882, 1308, 2239, 2317, 2937
BseNI ACTGG 7 cut(s) 430, 542, 983, 1438, 3205, 3958, 5171
BseRI GAGGAG 4 cut(s) 263, 3198, 4904, 4988
BseSI GKGCMC 4 cut(s) 609, 2454, 4461, 5119
BseYI CCCAGC 3 cut(s) 91, 3545, 5209
BsgI GTGCAG 6 cut(s) 60, 513, 1707, 3171, 3225, 3488
Bsh1236I CGCG 1 cut(s) 2667
Bsh1285I CGRYCG 1 cut(s) 4658
BshFI GGCC 8 cut(s) 1532, 2137, 3096, 3102, 3396, 4597, 4937, 5170
BshNI GGYRCC 3 cut(s) 2449, 3657, 4261
BsiEI CGRYCG 1 cut(s) 4658
BsiHKAI GWGCWC 5 cut(s) 2104, 2431, 4328, 4461, 5119
BsiSI CCGG 6 cut(s) 1527, 2794, 2878, 3097, 3433, 4178
BslFI GGGAC 5 cut(s) 237, 1065, 1111, 1430, 2343
BsmAI GTCTC 8 cut(s) 884, 1371, 2565, 2945, 4236, 4391, 4611, 4635
BsmBI CGTCTC 1 cut(s) 2945
BsmFI GGGAC 5 cut(s) 237, 1065, 1111, 1430, 2343
BsnI GGCC 8 cut(s) 1532, 2137, 3096, 3102, 3396, 4597, 4937, 5170
Bso31I GGTCTC 1 cut(s) 4611
Bsp119I TTCGAA 1 cut(s) 4475
Bsp1286I GDGCHC 8 cut(s) 315, 609, 2104, 2431, 2454, 4328, 4461, 5119
Bsp13I TCCGGA 2 cut(s) 2793, 4177
Bsp1407I TGTACA 2 cut(s) 3035, 3868
Bsp1720I GCTNAGC 1 cut(s) 4322
Bsp19I CCATGG 3 cut(s) 669, 3005, 5367
BspACI CCGC 8 cut(s) 536, 620, 887, 2134, 2515, 2665, 4414, 5576
BspANI GGCC 8 cut(s) 1532, 2137, 3096, 3102, 3396, 4597, 4937, 5170
BspEI TCCGGA 2 cut(s) 2793, 4177
BspFNI CGCG 1 cut(s) 2667
BspHI TCATGA 3 cut(s) 1807, 3604, 3616
BspMAI CTGCAG 2 cut(s) 2997, 3423
BspMI ACCTGC 2 cut(s) 1729, 3714
BspPI GGATC 8 cut(s) 727, 852, 1152, 1399, 3080, 3465, 4421, 5379
BspT104I TTCGAA 1 cut(s) 4475
BspT107I GGYRCC 3 cut(s) 2449, 3657, 4261
BspTNI GGTCTC 1 cut(s) 4611
BsrBI CCGCTC 1 cut(s) 4414
BsrDI GCAATG 6 cut(s) 16, 882, 1308, 2239, 2317, 2937
BsrFI RCCGGY 1 cut(s) 2877
BsrGI TGTACA 2 cut(s) 3035, 3868
BsrI ACTGG 7 cut(s) 430, 542, 983, 1438, 3205, 3958, 5171
BssAI RCCGGY 1 cut(s) 2877
BssNI GRCGYC 2 cut(s) 1240, 3658
BssSI CACGAG 3 cut(s) 489, 3243, 4068
BssT1I CCWWGG 6 cut(s) 669, 3005, 3103, 3666, 4315, 5367
Bst2BI CACGAG 3 cut(s) 489, 3243, 4068
Bst2UI CCWGG 9 cut(s) 414, 1587, 2115, 2142, 2565, 3807, 4622, 4939, 5490
Bst4CI ACNGT 7 cut(s) 579, 1984, 2035, 2207, 2324, 3196, 4656
BstACI GRCGYC 2 cut(s) 1240, 3658
BstAUI TGTACA 2 cut(s) 3035, 3868
BstBAI YACGTR 2 cut(s) 3392, 5114
BstBI TTCGAA 1 cut(s) 4475
BstC8I GCNNGC 8 cut(s) 2861, 2865, 3326, 3342, 3398, 3801, 3812, 5578
BstDSI CCRYGG 4 cut(s) 669, 3005, 4287, 5367
BstENI CCTNNNNNAGG 1 cut(s) 50
BstFNI CGCG 1 cut(s) 2667
BstH2I RGCGCY 4 cut(s) 2647, 3661, 5224, 5365
BstHHI GCGC 6 cut(s) 1693, 2646, 2669, 3660, 5223, 5364
BstMAI GTCTC 8 cut(s) 884, 1371, 2565, 2945, 4236, 4391, 4611, 4635
BstMCI CGRYCG 1 cut(s) 4658
BstNI CCWGG 9 cut(s) 414, 1587, 2115, 2142, 2565, 3807, 4622, 4939, 5490
BstNSI RCATGY 6 cut(s) 1384, 2336, 2738, 2863, 3814, 4095
BstPAI GACNNNNGTC 1 cut(s) 876
BstSFI CTRYAG 4 cut(s) 1011, 2993, 3419, 3483
BstSLI GKGCMC 4 cut(s) 609, 2454, 4461, 5119
BstUI CGCG 1 cut(s) 2667
BstV2I GAAGAC 2 cut(s) 801, 4920
BstX2I RGATCY 4 cut(s) 719, 844, 1391, 5371
BstXI CCANNNNNNTGG 1 cut(s) 1295
BstYI RGATCY 4 cut(s) 719, 844, 1391, 5371
BsuI GTATCC 3 cut(s) 1812, 2672, 5332
BsuRI GGCC 8 cut(s) 1532, 2137, 3096, 3102, 3396, 4597, 4937, 5170
BtgI CCRYGG 4 cut(s) 669, 3005, 4287, 5367
BtgZI GCGATG 1 cut(s) 3329
BtrI CACGTC 1 cut(s) 5440
BtsI GCAGTG 1 cut(s) 4126
BtsIMutI CAGTG 5 cut(s) 846, 1724, 1989, 3192, 4126
BveI ACCTGC 2 cut(s) 1729, 3714
Cac8I GCNNGC 8 cut(s) 2861, 2865, 3326, 3342, 3398, 3801, 3812, 5578
CaiI CAGNNNCTG 2 cut(s) 1663, 3212
CciI TCATGA 3 cut(s) 1807, 3604, 3616
CfoI GCGC 6 cut(s) 1693, 2646, 2669, 3660, 5223, 5364
Cfr10I RCCGGY 1 cut(s) 2877
CseI GACGC 3 cut(s) 1248, 1346, 5490
CspCI CAANNNNNGTGG 6 cut(s) 822, 857, 1589, 1624, 2753, 2788
DinI GGCGCC 1 cut(s) 3659
DraIII CACNNNGTG 1 cut(s) 5440
EaeI YGGCCR 3 cut(s) 3094, 3394, 4595
EciI GGCGGA 1 cut(s) 635
Eco130I CCWWGG 6 cut(s) 669, 3005, 3103, 3666, 4315, 5367
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 4611
Eco32I GATATC 1 cut(s) 5596
Eco47I GGWCC 7 cut(s) 95, 224, 253, 1052, 1417, 1856, 4180
Eco57I CTGAAG 5 cut(s) 2747, 3398, 3534, 3726, 5586
Eco72I CACGTG 2 cut(s) 3392, 5114
EcoNI CCTNNNNNAGG 1 cut(s) 50
EcoO109I RGGNCCY 3 cut(s) 1052, 1417, 3100
EcoRI GAATTC 1 cut(s) 4216
EcoRII CCWGG 9 cut(s) 412, 1585, 2113, 2140, 2563, 3805, 4620, 4937, 5488
EcoRV GATATC 1 cut(s) 5596
EcoT14I CCWWGG 6 cut(s) 669, 3005, 3103, 3666, 4315, 5367
EcoT22I ATGCAT 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 315
EgeI GGCGCC 1 cut(s) 3659
EheI GGCGCC 1 cut(s) 3659
ErhI CCWWGG 6 cut(s) 669, 3005, 3103, 3666, 4315, 5367
Esp3I CGTCTC 1 cut(s) 2945
FalI AAGNNNNNCTT 4 cut(s) 2856, 2888, 3646, 3678
FaqI GGGAC 5 cut(s) 237, 1065, 1111, 1430, 2343
FauI CCCGC 2 cut(s) 4407, 5569
FbaI TGATCA 3 cut(s) 967, 2287, 2592
FblI GTMKAC 2 cut(s) 2610, 5378
FriOI GRGCYC 1 cut(s) 315
FspBI CTAG 9 cut(s) 1262, 1269, 3104, 3297, 3533, 3593, 4224, 4866, 5599
GlaI GCGC 6 cut(s) 1692, 2645, 2668, 3659, 5222, 5363
GsaI CCCAGC 3 cut(s) 95, 3549, 5213
GsuI CTGGAG 2 cut(s) 447, 2163
HaeII RGCGCY 4 cut(s) 2647, 3661, 5224, 5365
HaeIII GGCC 8 cut(s) 1532, 2137, 3096, 3102, 3396, 4597, 4937, 5170
HapII CCGG 6 cut(s) 1527, 2794, 2878, 3097, 3433, 4178
HgaI GACGC 3 cut(s) 1248, 1346, 5490
HhaI GCGC 6 cut(s) 1693, 2646, 2669, 3660, 5223, 5364
Hin1I GRCGYC 2 cut(s) 1240, 3658
Hin6I GCGC 6 cut(s) 1691, 2644, 2667, 3658, 5221, 5362
HinP1I GCGC 6 cut(s) 1691, 2644, 2667, 3658, 5221, 5362
HincII GTYRAC 2 cut(s) 916, 4362
HindII GTYRAC 2 cut(s) 916, 4362
HpaII CCGG 6 cut(s) 1527, 2794, 2878, 3097, 3433, 4178
Hpy166II GTNNAC 8 cut(s) 916, 1508, 2611, 3038, 4362, 4459, 5117, 5379
Hpy8I GTNNAC 8 cut(s) 916, 1508, 2611, 3038, 4362, 4459, 5117, 5379
Hpy99I CGWCG 2 cut(s) 5441, 5555
HpyCH4III ACNGT 7 cut(s) 579, 1984, 2035, 2207, 2324, 3196, 4656
HpyCH4IV ACGT 6 cut(s) 2506, 3391, 4708, 4830, 5113, 5439
HpySE526I ACGT 6 cut(s) 2506, 3391, 4708, 4830, 5113, 5439
Hsp92I GRCGYC 2 cut(s) 1240, 3658
HspAI GCGC 6 cut(s) 1691, 2644, 2667, 3658, 5221, 5362
KasI GGCGCC 1 cut(s) 3657
KflI GGGWCCC 1 cut(s) 1417
Kpn2I TCCGGA 2 cut(s) 2793, 4177
KpnI GGTACC 1 cut(s) 4265
Ksp22I TGATCA 3 cut(s) 967, 2287, 2592
MaeI CTAG 9 cut(s) 1262, 1269, 3104, 3297, 3533, 3593, 4224, 4866, 5599
MaeII ACGT 6 cut(s) 2506, 3391, 4708, 4830, 5113, 5439
MbiI CCGCTC 1 cut(s) 4414
MflI RGATCY 4 cut(s) 719, 844, 1391, 5371
MhlI GDGCHC 8 cut(s) 315, 609, 2104, 2431, 2454, 4328, 4461, 5119
MlsI TGGCCA 1 cut(s) 3396
MluNI TGGCCA 1 cut(s) 3396
Mly113I GGCGCC 1 cut(s) 3658
MmeI TCCRAC 2 cut(s) 2784, 3889
Mox20I TGGCCA 1 cut(s) 3396
Mph1103I ATGCAT 1 cut(s) 554
MroI TCCGGA 2 cut(s) 2793, 4177
MroXI GAANNNNTTC 5 cut(s) 501, 1991, 2709, 3933, 4371
MscI TGGCCA 1 cut(s) 3396
MslI CAYNNNNRTG 7 cut(s) 923, 943, 1293, 1332, 1538, 1812, 1911
Msp20I TGGCCA 1 cut(s) 3396
MspA1I CMGCKG 5 cut(s) 538, 2354, 3418, 3704, 3713
MspI CCGG 6 cut(s) 1527, 2794, 2878, 3097, 3433, 4178
MvaI CCWGG 9 cut(s) 414, 1587, 2115, 2142, 2565, 3807, 4622, 4939, 5490
MvnI CGCG 1 cut(s) 2667
NarI GGCGCC 1 cut(s) 3658
NciI CCSGG 3 cut(s) 1528, 3098, 3434
NcoI CCATGG 3 cut(s) 669, 3005, 5367
NmeAIII GCCGAG 1 cut(s) 2645
NmuCI GTSAC 6 cut(s) 1019, 1334, 1540, 3506, 4417, 5528
NsiI ATGCAT 1 cut(s) 554
NspI RCATGY 6 cut(s) 1384, 2336, 2738, 2863, 3814, 4095
NspV TTCGAA 1 cut(s) 4475
OliI CACNNNNGTG 1 cut(s) 943
PaeI GCATGC 2 cut(s) 2863, 3814
PagI TCATGA 3 cut(s) 1807, 3604, 3616
PciI ACATGT 1 cut(s) 2332
PcsI WCGNNNNNNNCGW 1 cut(s) 3397
PdmI GAANNNNTTC 5 cut(s) 501, 1991, 2709, 3933, 4371
PflMI CCANNNNNTGG 2 cut(s) 1592, 2326
PfoI TCCNGGA 2 cut(s) 2140, 2563
PluTI GGCGCC 1 cut(s) 3661
PmaCI CACGTG 2 cut(s) 3392, 5114
PmlI CACGTG 2 cut(s) 3392, 5114
Ppu21I YACGTR 2 cut(s) 3392, 5114
PpuMI RGGWCCY 2 cut(s) 1052, 1417
PscI ACATGT 1 cut(s) 2332
PshAI GACNNNNGTC 1 cut(s) 876
PshBI ATTAAT 1 cut(s) 5015
PsiI TTATAA 2 cut(s) 4700, 5457
Psp5II RGGWCCY 2 cut(s) 1052, 1417
Psp6I CCWGG 9 cut(s) 412, 1585, 2113, 2140, 2563, 3805, 4620, 4937, 5488
PspCI CACGTG 2 cut(s) 3392, 5114
PspFI CCCAGC 3 cut(s) 91, 3545, 5209
PspGI CCWGG 9 cut(s) 412, 1585, 2113, 2140, 2563, 3805, 4620, 4937, 5488
PspPPI RGGWCCY 2 cut(s) 1052, 1417
PstI CTGCAG 2 cut(s) 2997, 3423
PstNI CAGNNNCTG 2 cut(s) 1663, 3212
PsuI RGATCY 4 cut(s) 719, 844, 1391, 5371
PvuII CAGCTG 4 cut(s) 2354, 3418, 3704, 3713
RseI CAYNNNNRTG 7 cut(s) 923, 943, 1293, 1332, 1538, 1812, 1911
ScaI AGTACT 2 cut(s) 2204, 4045
SduI GDGCHC 8 cut(s) 315, 609, 2104, 2431, 2454, 4328, 4461, 5119
SfcI CTRYAG 4 cut(s) 1011, 2993, 3419, 3483
SfoI GGCGCC 1 cut(s) 3659
SfuI TTCGAA 1 cut(s) 4475
SinI GGWCC 7 cut(s) 95, 224, 253, 1052, 1417, 1856, 4180
SmiMI CAYNNNNRTG 7 cut(s) 923, 943, 1293, 1332, 1538, 1812, 1911
SmlI CTYRAG 3 cut(s) 288, 1192, 3013
SmoI CTYRAG 3 cut(s) 288, 1192, 3013
SphI GCATGC 2 cut(s) 2863, 3814
SsiI CCGC 8 cut(s) 536, 620, 887, 2134, 2515, 2665, 4414, 5576
SspDI GGCGCC 1 cut(s) 3657
SspI AATATT 4 cut(s) 278, 437, 1108, 3055
SspMI CTAG 9 cut(s) 1262, 1269, 3104, 3297, 3533, 3593, 4224, 4866, 5599
StyI CCWWGG 6 cut(s) 669, 3005, 3103, 3666, 4315, 5367
TaaI ACNGT 7 cut(s) 579, 1984, 2035, 2207, 2324, 3196, 4656
TaiI ACGT 6 cut(s) 2509, 3394, 4711, 4833, 5116, 5442
TaqI TCGA 4 cut(s) 3181, 4475, 4566, 5287
TatI WGTACW 6 cut(s) 2202, 2258, 3035, 3868, 4043, 5409
TauI GCSGC 1 cut(s) 2137
TscAI CASTG 5 cut(s) 846, 1731, 1989, 3199, 4133
TseFI GTSAC 6 cut(s) 1019, 1334, 1540, 3506, 4417, 5528
Tsp45I GTSAC 6 cut(s) 1019, 1334, 1540, 3506, 4417, 5528
TspGWI ACGGA 4 cut(s) 2618, 2901, 4155, 4918
TspRI CASTG 5 cut(s) 846, 1731, 1989, 3199, 4133
Van91I CCANNNNNTGG 2 cut(s) 1592, 2326
VneI GTGCAC 2 cut(s) 4457, 5115
VpaK11BI GGWCC 7 cut(s) 95, 224, 253, 1052, 1417, 1856, 4180
VspI ATTAAT 1 cut(s) 5015
XagI CCTNNNNNAGG 1 cut(s) 50
XbaI TCTAGA 2 cut(s) 1268, 3532
XceI RCATGY 6 cut(s) 1384, 2336, 2738, 2863, 3814, 4095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1794
XmaJI CCTAGG 1 cut(s) 3103
XmiI GTMKAC 2 cut(s) 2610, 5378
XmnI GAANNNNTTC 5 cut(s) 501, 1991, 2709, 3933, 4371
XspI CTAG 9 cut(s) 1262, 1269, 3104, 3297, 3533, 3593, 4224, 4866, 5599
ZrmI AGTACT 2 cut(s) 2204, 4045
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.