Rw5G009130

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004130
Physical Location & Seq
Forward (+)
9837220 .. 9848885
11666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G009130.1

Sequence Viewer

Length: 5910 bp
ATGCAGCTGTTCACTCAGAAAGGACTAACTATTGGGTGCTTTCCGCGTAGACGTCCATTTTGGGAAAGCTTAGTAATTCTGCTCGTCATAGTGTTTTATGCATTCCAAGGAAGCCAGTTGGGAAAACATGGTGTGATGTTGATGCCAAAGAGGAGTTTGTCAGACCTTGTAATGATGTTAGATTGCATAACCAGCATAAATCGTCCAGGCATACAGTTGTACAATGTGGACTTGTTGAAACTGGAACCCTTGATGAAGCCTTACCAGTTTACTAGTGGGGATCTGAGAGTCTTGGCCACACTGGCTTCTCATACGACACACTTCCTCAACTCTTCGTCGTCTTCTATGGTGGAATCCGTCTGCTTTCTGAATGGAGGGTTAAGCATTTCTCAAATTAAACCAAAGTGGCCCAGTATGTCATTTGATAGATGTGATATTCGTCAACATTCCAAGCGATCCACTGGAGATTTTCAAATGTCACCAAATTCCATTAACCAAGATGCCAAGAGCTTCCTCGTTAATGCCATCAATATGAGTTTCTTAGAACTGGCTGAGCTGGCCATATACTTTACTAGAATGGACAAGACTTGGGCCAGCACAGGGCTTCAAAAGCAAACCCAAACCCTACCCTATGTAAAATGGGTTGGGCTAAGCCCGCTCAAGGGGCATGACCAACTCATAGCCCAAAAAATAAGGCTGGACTTGGTTATGCCTGTTGGGTCCTGGACTGATTTTAGCTTTCACAACATGGGTTGTAATTCAGATGGTGGGTCCCAAGTGTTTAGGGGTGAAGATGATGATGAAGAATATGAGGAGGATGGTAGGGGTAGTCGGTTTCTTGGGTTTATGTTTGGTAATGTGGATGACTCGGGTGATCTTGATGTTGATTACCTTGATGAGGATGCAAAGAAGCATTTTGCTGCACTTTCCAAGAAGCTTGGTCCTGCGTTCACGGATATGGACATGTCAGTGAAGTTACCAAGGACATTAAGCGATGCGATGGAGCAAGATTATGATGAGAAGGCTGAGAATGCTGTGAATTATTTTGACATTGATGAGGATTTTGAGGGTCCGGAGATTGAAGGTGCTACTGAGGAGGACCATCTTTTGCCAAGAGATGAATACCTTTCTGCTAAAGTTTCAGTGGATAAAATTCGAGAGGTGGAACATGATGTGGTTGAAAATTATGATGAGGAGAGTGAACAGGAAAAGGAACAAGAGGTGGTTGAACAGGATGTTAGAATTTCCCCACCGCTGCCAGTTCTATGCATAGAAGATGGAGAGGTGATCCTAAGATTCTCAGAAATCTTTGGCACTCATGTAAAGGAGGCAGTTAAAAGAAACCATGGGTACTCTGTTTGCAAAAGTATGCATAAATCCATGGATGTTACAGATATTGTTGAAGAGGATGAAGAGGCATTTCTCAAAGGAACTAGTGAAGGATTTACAGCTTTGAAAGAAGCTGATGGAGTGAAACATAACATCTCAGAATTGAATGATGATGATTTGGAATTTGCAAAATTTCGTGTTCACAAAGAGGCTACCTCATTTGCTGTGCTGGATGGGCCAGGAAAGGGCACGTACCTTTTTGCGGCACCAATGAAAGAAGATCTAGATGTGGATCTTTGTGTGGAAAGGCAGTCACCTTCGTGTCCTACATTTTACCCTCTTGATCAACAAGACTGGGAAGATGGAATTATTTGGGACAAGTCTCCTGTAATGAGTGACAATGCTGTTGAGAGTTGTGAAATGTCAGGACCTGACCACACCTCAGTTGATAGTGAAACAGAATCTGATAGTGGGTCGCAAAATATTCAGTTAGAGTCCCAAAAAGAGTCTGATGAGAAAAGGCATGATTGTATGCTATTTAGCCGGTCTAGTCTGTTGGAGCCTTTTGGCTCAAGAAATTCAGCAGGACCTTTATGTGTTCCTGTATCGGAAAGCAGATACCATCCCCAACTCTTAAGGCTAGAATCTCGATTTGAAGTAGATGACCAGGCAAATGGTAGAATGGAGGAAGTCGACCAGAAGCTTCATAAGAGCGATGCTGTGAGGCAATTCAGCAAGCTTACCTCACATAAAAGAGACATGCTGGAAGGATCGTGGTTAGACCAGATCATATGGGACCCAAACATGCCTAGTAGGAAGCCAAAGCTTATCCTTGATCTTGAAGATGAGCAAATGCTCTTTGAGATTTTGGATAACAAGGATAGTGAACATCTTAAGCTTCATTCAGGGGCCACGATTGTAACTCCCCCTCTAAAGTCAAGCAATGAGGATTCTTTGGATTTGCCCGGTCATGGATCTCAGTTTTGTTGGCGATATGTAGCTAATGACAAGCACTATGCAAACAGGAAAACATCTTATCAACTACAATCGAATTCTAAAAGACGCACTGCTCAAAGCATAAAGATTTATCACTCACAACCTGCACTTCGGCTACAGACTATGAAGCTGAAGTTAAGCAATAAGGAGGTTGCTAATTTTCATCGACCAAAGGCTTTATGGTATCCCTGCGACGATAAAAAAGCTGTAAAAGAACTAGGGAAGCTGCCTACTCAAGGACCAATGAAAATTATTATAAAGAGCTTGGGTGGCAAAGGCAGTAAGCTTCATGTTGATCATGAGGAAACTGTCTTTTCTGTCAAAGCAAAAGCTTCAAAAAAGCTAGATTTTAAGTCATCTGAAACAGTGAGGATGTTTTATAAAGGGAAGGAACTTGAAGATGATAAATCTCTAGCTGCTCAAAATGTTCAGCGGAACTCTTTGCTTTATCTGGTCCGTACAAATATATATTTGTTGCCAAGGTCGCAAAAACTTCCTGGTGAGAATAAATCGTTGCGTCCTCCTGGAGCATTTAAGAAGAAATCTGACCTTTCAGCTAAAGATGGCCATGTCTTTCTAGTAGAGTATTGTGAGGAAAGACCTTTACTTCTGAGCAATATTGGTATGGGTGCAAGATTGTGCACTTATTATCAAAATTCAGCCCCAGATGATCAAAATGGATCTTTGTTGCACAATGAAAACAGCAGCTCGGGTCATGTCATTTCGCTAAACCCTGCTGATAAATCACCTTTTCTTGGAGATATAAAAGCTGGTTGCTGTCGGTCATCTCTTGAGACAAACATGTATAGAGCACCTCTTTTTTCTCATAAGGTGCCGTCAACTGATTATCTGTTAGTTCGTTCTGCAAAGGGAAAGCTTTCAATTAGACGCATTGACAAGCTTAATGTTGTAGGGCAACAGGAACCTCTCATGAAGGTAATGTCTCCTGGAACTAAGAATCTGAAAAATTACATGCTTAACAGGCTCCTAGTCTACATTTGCCGTGAGTTCCGTGCTGCCGAAGAGCGCCATGTCCCTCCTTGTATTTGTGTAGATGAGTTACGTGCACAATTTCCTTACCTATCTGATCCCTTTATCAAAAACAAAGTGAAGGAACTTGTGAATTTGCCGAAGGGATCAAGTGGACATTGGGTTAAGAAGAGCAATTTCAGGATTTTCTCAGAGGATGAATTAAGAAATATGGTGAAACCAGAAGAGGTGTGTGCGTATGAAAGCATGCAAGCTGGTCTTTATCGCCTCAAACATTTAAGAATTACTCAAACTTATCCTTCTGTCATGTCATCTGCAATGAGTCATCTCCTCGACGAAGGTAATACTCTGGCTGCTGCATGTCACATCGAAAGGGAACTACAGACGACTCCATGGAATTTGAGTTGCAATTTTGTAGCCTGTACAATGGGAAAAGAAAATATTGAGCGTTTAGAAATATCTGGCCTTGGTGATCCATCTGGCCGGGGCCTAGGTTTTAGTTATGTTCGCGCTGCTCCAAAATCATCAATGTCCAATGCAGTGGGAAAGAAAAACTGTGCTACTGGTAGAGGACGTTCAGATTCTTTCAGCAGGGAGAGGTGTCAGGAAATTTGGGAACAGCAAGTTCATAGTCTTTCAGCAGTAGGCAGTGATGAGAATGAGACTGATTCTGAAGGAAATTATAGTGATCTGGATTCCTTTGCTGGGAGTTTAGAAAATCTGCTTGATGCAGAAGAATGTGAAAAGGGGTTAGGGGGTGACCATGAATTTGAGTGTGACAAAGAAGACGACGTTAAAGGGCTCGTAATGAGAAGGCTCCTGTCCTTGAATCCAGCAGAGGAAAATGAAGATGAAGCAGCTGAGTTATGCCGGTTGCTTATGGGTGATGAAACTGAGAGGAAGAAAAAGAAAACAACAAGAGTTTTGGAGGAAGCTAGGCTGCTTCCAGGCTCACGAACAAGTTTTACTTTTGAGAATGCAGATAGGGTAAAGCAAAACATTGTTGCAGCACAATTTGATGGTTCCTATACTCCTAAAGAGAATCCAATTGGAGACAGCAAAGTGATGGAAAATCTTCTTAAAAGAAGCAAGACTGGAGAATTAGAAGGGATGAGAGAGAAGGGTACTACTCCTATGCATAAGAAACTAAACGCATCAGGAAATGGTGTCAAGATGTTTGGTGAGAAGAAGCCAGTAAGAGAGAATTTTGTCTGTGGAGCCTGTGGTCAGCATGGACACATGAGGACAAACAAGAACTGCCCCAAGTTTGGAAAAGATCTGAAAGTGCACATTGAAACTCCAGATCTGGAGAATGTACTCGGAAGGTCCATTCCTCTGAACTCATCTAGTCAGTTTCAGCAGAAAACTACAACAAAGAAGCTGATACCAAAGACTGTAATTGATGTTGAAGGTTCTGAGGTTGAAAACCTTGGTCTGACCTCAAAGGTTGTTCCATTGACACTCAAAGTTGCGTTAGGTGAAAAGTTTCCTGATAAACAGGCTCCCGGAGAAACAGAGAGTTATCGGAATCCACTCACATCTGATCTTGAAACTGGGAATCCCGCTGTTAAGGTTAGTAAAATAATAATTTCTAACAAGACGAAACCTGGAGGGGTATCAGTTGAATCTCATAAACCCCCTATTGGGATATGGCCTCCCACAGATATAGATAGGGGTCATGTTAAGTCACAAAAGCCATTGCCAACTATAGTCATACGGCCTCCAGCAACCACAGTTAGAGATCATGTGGAATCTCAAAAGCCCTCTGTTACTAAACTGCCATTAGTGGAAGCTCACAAAGAGCAACATCATCAGAAAAGACATGAGAAGCTGAATAGCATGTATTTTCCTATTGAAGCAGCGAAAAAGAAAGCAAGAGATGACAGAAGATTGGGGGAGGAGCAAGAGAAACGAAGAATTGAAGAGAGTGCCAGGACGCTGTATGACAAGGAAATTAGGATGGCAAAGGAGCAAGAAAGACGTGCAGAGCTCAAAAAATATGAAGCAGCCATCAAACAAGAGAGAGAGGAAGAACTCCAGAAAGTGAAGAAGAATAAAATGATGCATAGAAGTCCACAGATAAACAATGACTATTTAGAGGACTCACGGACAAGAAGATTTGGTAAAAGGATGTTAAAAAGAGATCAGGGGGCCAAAAGGAGGCCTACTGTTGATTTGGGAAGTTATGGTGGAGGTTATGCCTCAACTACTAAGCGTCGTAAAGGAGGAGAGGTTGGTTTGGCAAACATCTTGGAGCGCATTGTAGAATCCCTCAAAGATCGAATTGAAATCTCGTATCTTTTTCTAAAACCAGTATCCAAAAAGGAAGCTCCTGATTACCGGGACATCATAGAGCACCCAATGGATCTGTCGACAATCAAGGTGAAGGTTCGGAACATGGAGTACAAGAGCCGGGAGCAATTCAGACAGGATGTGTGGCAGATTGCCCTTAATGCGCATAAATATAATGATGGGCGTAACCCGGATATCCCTCCACTTGCAGATGAGCTTTTAAACTTCTGTGACTATATGTTGGTTGAGAATGAAGAAAGCTTGACTGAAGCGGAAGCAGGCATTGAATATACAGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0000988 GO:0000989 GO:0001076 GO:0001129 GO:0001132 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0006139 GO:0006325 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016591 GO:0016740 GO:0016746 GO:0016747 GO:0017025 GO:0018130 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034212 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0055029 GO:0060255 GO:0061695 GO:0061733 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
Bromodomain DUF3591 TBP-binding ubiquitin zf-CCHC_6

Protein Analysis

1969

Amino Acids

222.76

Weight (kDa)

6.1

Isoelectric Point (pI)

50.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBP-binding PF09247 267 - 311 7.7e-10 TATA box-binding protein binding
DUF3591 PF12157 777 - 1302 1.4e-99 Protein of unknown function (DUF3591)
Rad60-SLD PF11976 857 - 924 7.5e-06 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 859 - 929 7.1e-08 Ubiquitin family
Bromodomain PF00439 1857 - 1936 2.5e-18 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2582, 2706
AatII GACGTC 1 cut(s) 55
Acc16I TGCGCA 1 cut(s) 5772
Acc36I ACCTGC 1 cut(s) 2437
AccB1I GGYRCC 2 cut(s) 1594, 3157
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 4 cut(s) 49, 2022, 3318, 5687
AccII CGCG 2 cut(s) 46, 3825
AccIII TCCGGA 1 cut(s) 1072
AciI CCGC 7 cut(s) 44, 656, 1253, 1592, 2758, 4878, 5879
AcoI YGGCCR 4 cut(s) 294, 558, 2890, 3796
AcuI CTGAAG 3 cut(s) 2477, 4008, 5895
AcyI GRCGYC 1 cut(s) 52
AfaI GTAC 8 cut(s) 221, 1352, 1583, 2785, 3739, 4441, 4632, 5720
AflII CTTAAG 2 cut(s) 1963, 2222
AflIII ACRYGT 2 cut(s) 963, 3126
AhlI ACTAGT 2 cut(s) 272, 1433
AjiI CACGTC 1 cut(s) 5297
AleI CACNNNNGTG 1 cut(s) 1648
Alw21I GWGCWC 6 cut(s) 2969, 3139, 3394, 4605, 5307, 5673
Alw26I GTCTC 6 cut(s) 1716, 2079, 3113, 3273, 3971, 4362
Alw44I GTGCAC 3 cut(s) 2965, 3390, 4601
AlwNI CAGNNNCTG 2 cut(s) 1760, 1793
Ama87I CYCGRG 2 cut(s) 868, 3034
Aor13HI TCCGGA 1 cut(s) 1072
ApaLI GTGCAC 3 cut(s) 2965, 3390, 4601
ArsI GACNNNNNNTTYG 4 cut(s) 42, 74, 4067, 4099
Asp700I GAANNNNTTC 3 cut(s) 3202, 4389, 4800
AspA2I CCTAGG 1 cut(s) 3805
AspLEI GCGC 4 cut(s) 3354, 3827, 5574, 5773
AsuC2I CCSGG 6 cut(s) 2295, 3800, 4821, 5657, 5729, 5798
AvaI CYCGRG 2 cut(s) 868, 3034
AvrII CCTAGG 1 cut(s) 3805
BaeGI GKGCMC 4 cut(s) 1580, 2969, 3394, 4605
BalI TGGCCA 3 cut(s) 296, 560, 2892
BanI GGYRCC 2 cut(s) 1594, 3157
BanII GRGCYC 2 cut(s) 4119, 5307
BbsI GAAGAC 2 cut(s) 333, 4107
Bbv12I GWGCWC 6 cut(s) 2969, 3139, 3394, 4605, 5307, 5673
BceAI ACGGC 3 cut(s) 3145, 3312, 5048
BciVI GTATCC 2 cut(s) 2520, 5641
BclI TGATCA 3 cut(s) 1672, 2620, 2995
BcnI CCSGG 6 cut(s) 2295, 3800, 4821, 5657, 5729, 5798
BcoDI GTCTC 6 cut(s) 1716, 2079, 3113, 3273, 3971, 4362
BcuI ACTAGT 2 cut(s) 272, 1433
BfmI CTRYAG 3 cut(s) 2441, 3695, 5022
BfoI RGCGCY 1 cut(s) 3355
BfrI CTTAAG 2 cut(s) 1963, 2222
BfuAI ACCTGC 1 cut(s) 2437
BfuI GTATCC 2 cut(s) 2520, 5641
BglI GCCNNNNNGGC 1 cut(s) 302
BglII AGATCT 3 cut(s) 1609, 4591, 4618
BlnI CCTAGG 1 cut(s) 3805
BlpI GCTNAGC 2 cut(s) 552, 650
BmeT110I CYCGRG 2 cut(s) 868, 3034
BmgBI CACGTC 1 cut(s) 5297
BmrI ACTGGG 3 cut(s) 405, 1693, 4878
BmsI GCATC 8 cut(s) 132, 490, 892, 985, 2035, 4033, 4478, 5367
BmuI ACTGGG 3 cut(s) 405, 1693, 4878
BpiI GAAGAC 2 cut(s) 333, 4107
BplI GAGNNNNNCTC 2 cut(s) 1529, 1561
BpmI CTGGAG 8 cut(s) 483, 2871, 4431, 4599, 4643, 4944, 5022, 5336
Bpu1102I GCTNAGC 2 cut(s) 552, 650
BpuEI CTTGAG 4 cut(s) 644, 1885, 2544, 3137
BpuMI CCSGG 6 cut(s) 2295, 3800, 4821, 5657, 5729, 5798
BsaAI YACGTR 2 cut(s) 1581, 3389
BsaBI GATNNNNATC 1 cut(s) 1620
BsaHI GRCGYC 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 1072
BsaXI ACNNNNNCTCC 2 cut(s) 5532, 5562
Bse118I RCCGGY 2 cut(s) 1872, 4185
Bse3DI GCAATG 3 cut(s) 2278, 3639, 5012
Bse8I GATNNNNATC 1 cut(s) 1620
BseAI TCCGGA 1 cut(s) 1072
BseJI GATNNNNATC 1 cut(s) 1620
BseMI GCAATG 3 cut(s) 2278, 3639, 5012
BseRI GAGGAG 7 cut(s) 166, 827, 1109, 1208, 3635, 5228, 5556
BseSI GKGCMC 4 cut(s) 1580, 2969, 3394, 4605
BseYI CCCAGC 1 cut(s) 4019
BsgI GTGCAG 3 cut(s) 906, 2415, 5319
Bsh1236I CGCG 2 cut(s) 46, 3825
BshNI GGYRCC 2 cut(s) 1594, 3157
BsiHKAI GWGCWC 6 cut(s) 2969, 3139, 3394, 4605, 5307, 5673
BsiHKCI CYCGRG 2 cut(s) 868, 3034
BsiSI CCGG 9 cut(s) 1073, 1873, 2295, 3799, 4186, 4821, 5656, 5728, 5798
BslFI GGGAC 6 cut(s) 757, 1718, 1810, 2138, 3344, 5672
BsmAI GTCTC 6 cut(s) 1716, 2079, 3113, 3273, 3971, 4362
BsmFI GGGAC 6 cut(s) 757, 1718, 1810, 2138, 3344, 5672
BsmI GAATGC 3 cut(s) 101, 1036, 4297
BsoBI CYCGRG 2 cut(s) 868, 3034
Bsp1286I GDGCHC 8 cut(s) 1580, 2969, 3139, 3394, 4119, 4605, 5307, 5673
Bsp13I TCCGGA 1 cut(s) 1072
Bsp1407I TGTACA 2 cut(s) 219, 3737
Bsp1720I GCTNAGC 2 cut(s) 552, 650
Bsp19I CCATGG 3 cut(s) 1345, 1380, 3707
BspACI CCGC 7 cut(s) 44, 656, 1253, 1592, 2758, 4878, 5879
BspEI TCCGGA 1 cut(s) 1072
BspFNI CGCG 2 cut(s) 46, 3825
BspHI TCATGA 2 cut(s) 2623, 3255
BspMI ACCTGC 1 cut(s) 2437
BspQI GCTCTTC 2 cut(s) 3342, 3479
BspT107I GGYRCC 2 cut(s) 1594, 3157
BspTI CTTAAG 2 cut(s) 1963, 2222
BsrBI CCGCTC 1 cut(s) 658
BsrDI GCAATG 3 cut(s) 2278, 3639, 5012
BsrFI RCCGGY 2 cut(s) 1872, 4185
BsrGI TGTACA 2 cut(s) 219, 3737
BssAI RCCGGY 2 cut(s) 1872, 4185
BssNI GRCGYC 1 cut(s) 52
BssT1I CCWWGG 9 cut(s) 106, 980, 1345, 1380, 2804, 3707, 3781, 3805, 4744
Bst4CI ACNGT 7 cut(s) 216, 2635, 2692, 3872, 4711, 5050, 5485
Bst6I CTCTTC 7 cut(s) 337, 1398, 1407, 3342, 3479, 3534, 5232
BstACI GRCGYC 1 cut(s) 52
BstAFI CTTAAG 2 cut(s) 1963, 2222
BstAUI TGTACA 2 cut(s) 219, 3737
BstBAI YACGTR 2 cut(s) 1581, 3389
BstC8I GCNNGC 7 cut(s) 558, 595, 656, 2066, 3563, 3567, 5887
BstDSI CCRYGG 3 cut(s) 1345, 1380, 3707
BstEII GGTNACC 1 cut(s) 4073
BstENI CCTNNNNNAGG 2 cut(s) 896, 2559
BstFNI CGCG 2 cut(s) 46, 3825
BstH2I RGCGCY 1 cut(s) 3355
BstHHI GCGC 4 cut(s) 3354, 3827, 5574, 5773
BstMAI GTCTC 6 cut(s) 1716, 2079, 3113, 3273, 3971, 4362
BstNSI RCATGY 8 cut(s) 967, 2092, 2137, 3130, 3301, 3565, 3678, 5158
BstPI GGTNACC 1 cut(s) 4073
BstSFI CTRYAG 3 cut(s) 2441, 3695, 5022
BstSLI GKGCMC 4 cut(s) 1580, 2969, 3394, 4605
BstUI CGCG 2 cut(s) 46, 3825
BstV2I GAAGAC 2 cut(s) 333, 4107
BstX2I RGATCY 8 cut(s) 280, 1609, 1621, 2303, 3005, 4591, 4618, 5680
BstXI CCANNNNNNTGG 2 cut(s) 2003, 3856
BstYI RGATCY 8 cut(s) 280, 1609, 1621, 2303, 3005, 4591, 4618, 5680
BsuI GTATCC 2 cut(s) 2520, 5641
BtgI CCRYGG 3 cut(s) 1345, 1380, 3707
BtgZI GCGATG 3 cut(s) 1008, 1013, 2058
BtrI CACGTC 1 cut(s) 5297
BtsI GCAGTG 3 cut(s) 2394, 3861, 3970
BtsIMutI CAGTG 8 cut(s) 299, 459, 975, 1149, 2394, 2697, 3861, 3970
BveI ACCTGC 1 cut(s) 2437
Cac8I GCNNGC 7 cut(s) 558, 595, 656, 2066, 3563, 3567, 5887
CaiI CAGNNNCTG 2 cut(s) 1760, 1793
CciI TCATGA 2 cut(s) 2623, 3255
CfoI GCGC 4 cut(s) 3354, 3827, 5574, 5773
Cfr10I RCCGGY 2 cut(s) 1872, 4185
CseI GACGC 5 cut(s) 2400, 2831, 3222, 5260, 5519
Csp6I GTAC 8 cut(s) 220, 1351, 1582, 2784, 3738, 4440, 4631, 5719
CviQI GTAC 8 cut(s) 220, 1351, 1582, 2784, 3738, 4440, 4631, 5719
DraI TTTAAA 1 cut(s) 5829
EaeI YGGCCR 4 cut(s) 294, 558, 2890, 3796
Eam1104I CTCTTC 7 cut(s) 337, 1398, 1407, 3342, 3479, 3534, 5232
EarI CTCTTC 7 cut(s) 337, 1398, 1407, 3342, 3479, 3534, 5232
Ecl136II GAGCTC 1 cut(s) 5305
Eco130I CCWWGG 9 cut(s) 106, 980, 1345, 1380, 2804, 3707, 3781, 3805, 4744
Eco147I AGGCCT 1 cut(s) 5479
Eco24I GRGCYC 2 cut(s) 4119, 5307
Eco32I GATATC 1 cut(s) 5803
Eco53kI GAGCTC 1 cut(s) 5305
Eco57I CTGAAG 3 cut(s) 2477, 4008, 5895
Eco88I CYCGRG 2 cut(s) 868, 3034
Eco91I GGTNACC 1 cut(s) 4073
EcoICRI GAGCTC 1 cut(s) 5305
EcoNI CCTNNNNNAGG 2 cut(s) 896, 2559
EcoO109I RGGNCCY 6 cut(s) 720, 771, 1757, 1916, 2125, 3802
EcoO65I GGTNACC 1 cut(s) 4073
EcoRI GAATTC 1 cut(s) 2380
EcoRV GATATC 1 cut(s) 5803
EcoT14I CCWWGG 9 cut(s) 106, 980, 1345, 1380, 2804, 3707, 3781, 3805, 4744
EcoT22I ATGCAT 5 cut(s) 103, 1271, 1374, 4455, 5382
EcoT38I GRGCYC 2 cut(s) 4119, 5307
ErhI CCWWGG 9 cut(s) 106, 980, 1345, 1380, 2804, 3707, 3781, 3805, 4744
FalI AAGNNNNNCTT 4 cut(s) 3558, 3590, 4267, 4299
FaqI GGGAC 6 cut(s) 757, 1718, 1810, 2138, 3344, 5672
FauI CCCGC 2 cut(s) 663, 4885
FauNDI CATATG 1 cut(s) 2120
FbaI TGATCA 3 cut(s) 1672, 2620, 2995
FblI GTMKAC 4 cut(s) 49, 2022, 3318, 5687
FriOI GRGCYC 2 cut(s) 4119, 5307
FspAI RTGCGCAY 1 cut(s) 5772
FspI TGCGCA 1 cut(s) 5772
GlaI GCGC 4 cut(s) 3353, 3826, 5573, 5772
GsaI CCCAGC 1 cut(s) 4023
GsuI CTGGAG 8 cut(s) 483, 2871, 4431, 4599, 4643, 4944, 5022, 5336
HaeII RGCGCY 1 cut(s) 3355
HapII CCGG 9 cut(s) 1073, 1873, 2295, 3799, 4186, 4821, 5656, 5728, 5798
HgaI GACGC 5 cut(s) 2400, 2831, 3222, 5260, 5519
HhaI GCGC 4 cut(s) 3354, 3827, 5574, 5773
Hin1I GRCGYC 1 cut(s) 52
Hin6I GCGC 4 cut(s) 3352, 3825, 5572, 5771
HinP1I GCGC 4 cut(s) 3352, 3825, 5572, 5771
HincII GTYRAC 4 cut(s) 443, 2023, 3165, 5688
HindII GTYRAC 4 cut(s) 443, 2023, 3165, 5688
HpaII CCGG 9 cut(s) 1073, 1873, 2295, 3799, 4186, 4821, 5656, 5728, 5798
Hpy99I CGWCG 5 cut(s) 340, 2522, 3653, 4109, 5535
HpyCH4III ACNGT 7 cut(s) 216, 2635, 2692, 3872, 4711, 5050, 5485
HpyCH4IV ACGT 6 cut(s) 52, 1580, 3388, 3889, 4107, 5296
HpySE526I ACGT 6 cut(s) 52, 1580, 3388, 3889, 4107, 5296
Hsp92I GRCGYC 1 cut(s) 52
HspAI GCGC 4 cut(s) 3352, 3825, 5572, 5771
KflI GGGWCCC 2 cut(s) 771, 2125
Kpn2I TCCGGA 1 cut(s) 1072
Ksp22I TGATCA 3 cut(s) 1672, 2620, 2995
LguI GCTCTTC 2 cut(s) 3342, 3479
LweI GCATC 8 cut(s) 132, 490, 892, 985, 2035, 4033, 4478, 5367
MaeII ACGT 6 cut(s) 52, 1580, 3388, 3889, 4107, 5296
MbiI CCGCTC 1 cut(s) 658
MfeI CAATTG 1 cut(s) 4362
MflI RGATCY 8 cut(s) 280, 1609, 1621, 2303, 3005, 4591, 4618, 5680
MhlI GDGCHC 8 cut(s) 1580, 2969, 3139, 3394, 4119, 4605, 5307, 5673
MlsI TGGCCA 3 cut(s) 296, 560, 2892
MluNI TGGCCA 3 cut(s) 296, 560, 2892
MlyI GAGTC 7 cut(s) 297, 860, 1832, 1844, 3646, 3697, 5411
MmeI TCCRAC 1 cut(s) 1866
Mox20I TGGCCA 3 cut(s) 296, 560, 2892
Mph1103I ATGCAT 5 cut(s) 103, 1271, 1374, 4455, 5382
MroI TCCGGA 1 cut(s) 1072
MroXI GAANNNNTTC 3 cut(s) 3202, 4389, 4800
MscI TGGCCA 3 cut(s) 296, 560, 2892
MslI CAYNNNNRTG 4 cut(s) 530, 956, 968, 1648
Msp20I TGGCCA 3 cut(s) 296, 560, 2892
MspA1I CMGCKG 5 cut(s) 7, 1255, 2758, 4175, 4880
MspCI CTTAAG 2 cut(s) 1963, 2222
MspI CCGG 9 cut(s) 1073, 1873, 2295, 3799, 4186, 4821, 5656, 5728, 5798
MunI CAATTG 1 cut(s) 4362
Mva1269I GAATGC 3 cut(s) 101, 1036, 4297
MvnI CGCG 2 cut(s) 46, 3825
NciI CCSGG 6 cut(s) 2295, 3800, 4821, 5657, 5729, 5798
NcoI CCATGG 3 cut(s) 1345, 1380, 3707
NdeI CATATG 1 cut(s) 2120
NmuCI GTSAC 8 cut(s) 477, 1641, 1724, 3677, 4073, 4091, 5001, 5837
NsbI TGCGCA 1 cut(s) 5772
NsiI ATGCAT 5 cut(s) 103, 1271, 1374, 4455, 5382
NspI RCATGY 8 cut(s) 967, 2092, 2137, 3130, 3301, 3565, 3678, 5158
OliI CACNNNNGTG 1 cut(s) 1648
PaeI GCATGC 1 cut(s) 3565
PagI TCATGA 2 cut(s) 2623, 3255
PceI AGGCCT 1 cut(s) 5479
PciI ACATGT 2 cut(s) 963, 3126
PciSI GCTCTTC 2 cut(s) 3342, 3479
PctI GAATGC 3 cut(s) 101, 1036, 4297
PdmI GAANNNNTTC 3 cut(s) 3202, 4389, 4800
PfoI TCCNGGA 4 cut(s) 722, 2848, 3271, 4819
PleI GAGTC 7 cut(s) 296, 860, 1831, 1843, 3645, 3697, 5411
PpsI GAGTC 7 cut(s) 296, 860, 1831, 1843, 3645, 3697, 5411
Ppu21I YACGTR 2 cut(s) 1581, 3389
PpuMI RGGWCCY 5 cut(s) 720, 771, 1757, 1916, 2125
PscI ACATGT 2 cut(s) 963, 3126
PsiI TTATAA 2 cut(s) 2582, 2706
Psp124BI GAGCTC 1 cut(s) 5307
Psp5II RGGWCCY 5 cut(s) 720, 771, 1757, 1916, 2125
PspEI GGTNACC 1 cut(s) 4073
PspFI CCCAGC 1 cut(s) 4019
PspPPI RGGWCCY 5 cut(s) 720, 771, 1757, 1916, 2125
PsrI GAACNNNNNNTAC 6 cut(s) 3874, 3906, 4264, 4296, 5702, 5734
PstNI CAGNNNCTG 2 cut(s) 1760, 1793
PsuI RGATCY 8 cut(s) 280, 1609, 1621, 2303, 3005, 4591, 4618, 5680
PvuII CAGCTG 2 cut(s) 7, 4175
RsaI GTAC 8 cut(s) 221, 1352, 1583, 2785, 3739, 4441, 4632, 5720
RsaNI GTAC 8 cut(s) 220, 1351, 1582, 2784, 3738, 4440, 4631, 5719
RseI CAYNNNNRTG 4 cut(s) 530, 956, 968, 1648
SacI GAGCTC 1 cut(s) 5307
SalI GTCGAC 2 cut(s) 2021, 5686
SapI GCTCTTC 2 cut(s) 3342, 3479
SchI GAGTC 7 cut(s) 297, 860, 1832, 1844, 3646, 3697, 5411
SduI GDGCHC 8 cut(s) 1580, 2969, 3139, 3394, 4119, 4605, 5307, 5673
SfaNI GCATC 8 cut(s) 132, 490, 892, 985, 2035, 4033, 4478, 5367
SfcI CTRYAG 3 cut(s) 2441, 3695, 5022
SmiMI CAYNNNNRTG 4 cut(s) 530, 956, 968, 1648
SmlI CTYRAG 6 cut(s) 659, 1900, 1963, 2222, 2559, 3116
SmoI CTYRAG 6 cut(s) 659, 1900, 1963, 2222, 2559, 3116
SpeI ACTAGT 2 cut(s) 272, 1433
SphI GCATGC 1 cut(s) 3565
SseBI AGGCCT 1 cut(s) 5479
SsiI CCGC 7 cut(s) 44, 656, 1253, 1592, 2758, 4878, 5879
SspI AATATT 3 cut(s) 1813, 2944, 3757
SstI GAGCTC 1 cut(s) 5307
StuI AGGCCT 1 cut(s) 5479
StyI CCWWGG 9 cut(s) 106, 980, 1345, 1380, 2804, 3707, 3781, 3805, 4744
TaaI ACNGT 7 cut(s) 216, 2635, 2692, 3872, 4711, 5050, 5485
TaiI ACGT 6 cut(s) 55, 1583, 3391, 3892, 4110, 5299
TaqI TCGA 9 cut(s) 1156, 1978, 2022, 2378, 2491, 3648, 3684, 5596, 5687
TaqII GACCGA 1 cut(s) 3096
TatI WGTACW 4 cut(s) 219, 3737, 4630, 5718
TauI GCSGC 1 cut(s) 1595
TscAI CASTG 8 cut(s) 306, 466, 975, 1149, 2401, 2697, 3861, 3970
TseFI GTSAC 8 cut(s) 477, 1641, 1724, 3677, 4073, 4091, 5001, 5837
Tsp45I GTSAC 8 cut(s) 477, 1641, 1724, 3677, 4073, 4091, 5001, 5837
TspGWI ACGGA 5 cut(s) 346, 968, 2771, 3326, 5437
TspRI CASTG 8 cut(s) 306, 466, 975, 1149, 2401, 2697, 3861, 3970
Vha464I CTTAAG 2 cut(s) 1963, 2222
VneI GTGCAC 3 cut(s) 2965, 3390, 4601
XagI CCTNNNNNAGG 2 cut(s) 896, 2559
XbaI TCTAGA 1 cut(s) 1612
XceI RCATGY 8 cut(s) 967, 2092, 2137, 3130, 3301, 3565, 3678, 5158
XcmI CCANNNNNNNNNTGG 2 cut(s) 272, 2502
XmaJI CCTAGG 1 cut(s) 3805
XmiI GTMKAC 4 cut(s) 49, 2022, 3318, 5687
XmnI GAANNNNTTC 3 cut(s) 3202, 4389, 4800
ZraI GACGTC 1 cut(s) 53
Zsp2I ATGCAT 5 cut(s) 103, 1271, 1374, 4455, 5382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.