Rroxscaffold_1G00063080

Transcription initiation factor TFIID subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004130
Physical Location & Seq
Reverse (-)
85051222 .. 85057908
6687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063080.1

Sequence Viewer

Length: 5157 bp
ATGGGTTGTAATTCAGATGGTGGGTCCCAAGTGTTTTGGGGTGAAGATGATGATGAAGAATATGAGGAGGATGGTAGGGGTAGTCGGTTTCTTGGGTTTATGTTTGGTAATGTGGATGACTCGGGTGATCTTGATGTTGATTACCTTGATGAGGATGCAAAGAAGCATTTTGCTGCACTTTCCGAGGAGCTTGGTCCTGCTTTCACGGATATGGACATGTCAGTGAAGTTACCAAGGACATTAAGCGATGCGGTGGAGCAAGATTATGATGAGAAGGCTGAGAATGCTGTGAATTATTTTGACATTGATGAGGATTTTGAGGGTCCGGAGATTGAAGGTGCTACTGAGGAGGACCATCTTTTGCCGAGAGATGAATACCTTTCTGCTAAAGTTTCGGTGGATAAAATTCGAGAAGTGGAACATGAGGTGGTTGAAAATTATGATGAGGAGAGTGAACAGGAAAAGGAACAAGAGGTGGTTGAACAGGATGTTAGAATTTCCCCAGCGCTGCCAGTTCTATGCATAGAAGATGGAGAGGTGATCCTAAGATTCTCAGAAATCTTTGGCACTCATGTAAAGGAGGCAGTTAAAAGAAACCATAGGTACTCTGTTTGCAAAAGTATGCATAAATCCATGGATGTTACGGATATTGTTGAAGAGGATGAAGAGGCATTTCTCAAAGGAACTGGTGAAGGGTTTACAGCTTTGAAAGAAGCTGATGGAGTGAAACATAACATCTCAGAATTGAACGATGATGATTTGGAATTTGCAAAATTTCGTATTCACAAAGAGGCTACCTCATTTGCTGTGCTGGATGGGCCAGGAAAGGGCACGTACCTTTTTGCGGCACCAATGAAAGAAGATCTAGATGTGGATCTTTGTGTGGAAAGGCACTCACCTTCGTGTCCTACATTTTACCCTCTTGATCAACAAGTCTGGGAAGATGGAATTATTTGGGACAAGTCTCCTGTAATGAGTGACAATGCTGTTGAGAGTTGTGAAATGTCAGGACCTGACCACACCTCAGTTGATAGTGAAACAGAATCTGATAGTGGGTCGCAAAATATTCAGTTAGAGTCCCAAAAAGAGTCTGATGAGAAAAGGCATGACATTATGCTATTTAGCCGGTCTAGTCTGTTGGAGCCTTTTGGCTCAAGAAATTCAGCAGGACCTTTATGTGTTCCTGTATCGGAAAGCAGATACCATCCCCAACTCTTAAGGCTAGAATCTCGATTTGAAGTGGATGACCAGGCAAATGGTAGAATGGAGAATGTCGATCAGAAGCTTCGTAAGAGAGATGCTGTGAGGCAATTCAGCAAGCTTACCTCACATAACAGAGCCATGCTAAAAGGATCGTGGTTAGACCAGATCATATGGGACCCAAACATGCCTATTAGGAAGCCAAAGCTTATCCTTGATCTTGAAGATGAGCAAATGCTCTTTGAGATTTTGGATAACAAGGATAGTGAACATCTGAAGCCTCATTCAGGGGCCACGATTGTAACTTCCCCTCTAAAGTCAAGCAATGAGGATTCTTTGGATTTGCCCAGTCATGGAGCTCAGTTTTGTTGGCGATATGTAGCTAATGACAAGCACTATGCAAACAGGAAAACATCTTATCAACTACAATCGAATTCTAAAAGACGCACTGCTCAAAGCATAAAGATTTATCACTCACAACCTGCACTTCGGCTACAGACTATGAAGCTGAAGTTAAGCAACTTTCTTGTGCTTGTACATGATAGTAAGGAGGTTGCTAATTTTCATCGACCAAAAGCTTTATGGTATCCCTCCGACGATGAAAAAGCTGTAAAAGAACTAGGGAAGCTGCCTACTCAAGGACCAATGAAAATTATTATAAAGAGCTTGGGTGGAAAAGGCAGTAAGCTTCATGTTGATCATGAGGAAACTGTCTTTTCTGTCAAAGCAAAAGCTTCAAAAAAGCTAGATTTTAAGTCATCTGAAACAGTGAGGATGTTTTATAAAGGGAAGGAACTTGAAGATGATAAATCTCTAGCTGCTCAAAATGTTCGGCGGAACTCTTTGCTTTATCTGGTCCGTACAAATATATATTTGTTGCCAAGGTCGCAAAAACTTCCTGGTGAGAATAAATCGTTGCGTCCTCCTGGAGCATTTAAGAAGAAATCTGACCTTTCAGCGAAAGATGGTCATGTCTTTCTAGTAGAGTATTGTGAGGAAAGGCCTTTACTTCTGAGCAATATTGGTATGGGTGCAAGATTGTGCACTTATTATCAAAATTCAGCCGCAGATGATCAAAATGGATCTTTGTTGCACAATGAAAACAGCAGCTTGGATCTTGTCATTTCGCTAAACCCTGCTGATAAATCACCTTTTCTTGGAGATATAAAAGCTGGTTGCTGTCAGTCATCTCTTGAGACAAACATGTATAGAGCACCTCTTTTTTCTCATAAGGTGCCATCAACTGATTATCTGTTAGTTCGTTCTGCAAAGGGAAAGCTTTCAATTAGACGCATTGACAAGCTCAATGTTGTAGGGCAACAGGAACCTCTCATGAAGGTAATGTCTCCTGGAACTAAGAATCTGAAAAATTACATGCTTAACAGGCTTCGAGTCTACATGTGCCGTGAGTTCCGTGCTGCCGAAGAGCGCCATGTCCCTCCTTGTATTTGTGTAGATGAGTTACGTGCACAATTTCCTTACCTATCTGATCCCTTTATCAAAAACAAAGTGAAGGAACTTGTGAATTTGCCGAAGGGATCAAGTGGACATTGGGTTAAGAAGAGCAATTTCAGGATTTTCTCAGAGGATGAATTAAGAAATATGGTGAAACCAGAAGAGGTGTGTGCCTATGAAAGCATGCAAGCTGGTCTTTATCGGCTCAAACATTTACGGATTACTCAAACTTATCCTTCTGTCATGTCATCTGCAATGAGTCATCTCCACGACGAAGGTAATACTCTGACTGCTGCATGTCACATCGAAAGGGAACTACAGACGACTCCATGGAATTTGAGTTGCAATTTTGTAGCGTGTACAATGGGAAAAGAAAATATTGAGCGTTTAGAAATATCTGGCCTTGGTGATCCATCTGGCCGGGGCTTAGGTTTTAGTTATGTTCGCGCTGCTCCAAAATCATCAATGTCCAATGCAGTGGGAAAGAAAAACTGTGCTACTGGTAGAGGACGTTCAGATTCTTTCAGCAGGGAGAGGTGTCAGGAAATTTGGGAACAGCAAGTTCATAGTCTTTCAGCAGTAGGCAGTGATGAGAATGAGAGTGACTCTGAAGGAAATTATAGTGATCTGGATTCCTTTGCTGGGAGTTTAGAAAATCTGCTTGATGCAGAAGAATGTGAAAAGGGGTTAGGGGGTGACCATGAATTTGAGTGTGACAAAGAAGACGACGTTAAAGGGCTCGTAATGAGAAGGCTCCTGTCCTTGAATCCAGCAGAGGAAAGTGAAGATGAAGCAGCTGAAGCAGCTGAGTTATGCCGGTTGCTTATGGGTGATGAAACTGAGAGGAAGAAAAAGAAAACAACAAGAGTTTTGGAGGAAGTTAGGCTGCTTCCAGGCTCACGAACAAGTTTTGCTTTTGAGAATTCAGATAGGGTAAAGCAAAACATTGTTGCAGCACAATTTGATGGTTCCTATACTCCTAAAGAGAATCCAATTGGAGACAGCAAAGTGATGGAAAATCTTCTTAAAAGAAGCAAGACTGGAGAATTAGAAGGGATGAGAGAGAATGGTACTACACCGATGCATGAGAAACTAAACACATCAGGAAATGGTGTCAAGATGTTTGGTGAGAAGAAGCCAGTAAGAGAGAATTTTGTCTGTGGAGCATGTGGTCAGCATGGACACATGAGGACAAACAAGAACTGCCCCAAGTTTGGAAAAGATCTCAAAGTGCACATTGAAACTCCAGATCTGGAGAATGTACTTGGAAGGTCCATTCCTCTGAACTCATCTAGTCGGTTTCAGCAGAAAACTACAACAAAGAAGCTGATACCAAAGACTGTAATTGATGTTGAAGGTTCTGAGGTTGAAAACCTTGGTCTGACCTCAAAGGTTGTTCCATTGACACTCAAAGTTGCGTTAGGTGAAAAGTTTCCTGATAAACAGGCTCCTGGAGAAACAGAGAGTTATCGGAATCCACTCACATCTGATCTTGAAACTGGGAATCCTGCTGTTAAGGTTAGTAAAATAATAATTTCTAACAAGACGAAACCTGGAGGGGTATCAGTTGAATCTCATAAACCCCCGATTGGGATATGGCCTCCCACAGATATAGATAGGGGTCATGTTAAGTCACAAAAGCCATTGCCAACTATAGTCATACGGCCTCCAGCAACCACAGTTAGAGATCATGTGGAATCTCAAAAGCCCTCTGTTGCTAAACTGCCAATGGTGGAAGCTCACAAAGAGCAACATCATCAGAAAAGACATGAGAAGCTGAATAGCATGTATTTTCCTCTTGAAGCAGCGAAAAAGAAAGCAAGAGATGATAGAAGATTGGGGGAGGAGCAAGAGAAACGAAGAATTGAAGGGAGTGCCAGGAGGCTGTATGACAAGGAAATTAGGATGGCAAAGGAGCAAGAAAGACGTGCAGAGCTCAAAAAATATGAAGCAGCCATCAAACAAGAGAGAGAGGAAGAACTCCAGAAAGTGAAGAAGAATAAAATGATGCATAGAAGTCCACAGATAAACGATGACTATTTAGAGGACTCCCGGACAAGAAGATTTGGTAAAAGGATGCCAGAAAGAGATCGGGGGCCAAAAGGAGGCCAACTGTTGATTTGGGAAGTTATGGCGGAGGTTGGTTTGGCAAACATCTTGGAGCGCATTGTAGAATCCCTCAAAGATCGAATTGAAATCTCGTATCTTTTTTTAAAACCAGTATCCAAAAAGGAAGCTCCGGATTACCGGGACATCATAAAGCACCCAATGGATCTGTCGACAATCAAGGTGAAGGTTCGGAATATGGAGTACAAGAGCCGGGAGCAATTCAGACGGGATGTGTGGCAGATTGCCTTTAATGCCCATAAATATAATGATGAGCATAACCCGGATATCCCTCCACTTGCAGATCAGCTTTTAAACTTCTGTGACTATATGTTGGTTGAGAATGAAGAAAGTCTGACTGAAGCGGAAGCAGGCATTGAATATGCAGATATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0000988 GO:0000989 GO:0001076 GO:0001129 GO:0001132 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0006139 GO:0006325 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016569 GO:0016570 GO:0016573 GO:0016591 GO:0016740 GO:0016746 GO:0016747 GO:0017025 GO:0018130 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034212 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0055029 GO:0060255 GO:0061695 GO:0061733 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 map03022
Pfam Domains
Protein Families
Bromodomain DUF3591 TBP-binding ubiquitin zf-CCHC_6

Protein Analysis

1718

Amino Acids

194.73

Weight (kDa)

5.7

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TBP-binding PF09247 18 - 62 1.8e-09 TATA box-binding protein binding
DUF3591 PF12157 528 - 1061 1.1e-96 Protein of unknown function (DUF3591)
ubiquitin PF00240 618 - 688 1.8e-07 Ubiquitin family
Bromodomain PF00439 1606 - 1681 2.2e-18 Bromodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1859, 1983
Acc36I ACCTGC 1 cut(s) 1690
AccB1I GGYRCC 2 cut(s) 847, 2434
AccI GTMKAC 2 cut(s) 2595, 4934
AccII CGCG 1 cut(s) 3102
AccIII TCCGGA 2 cut(s) 325, 4894
AciI CCGC 6 cut(s) 251, 845, 2035, 2265, 4790, 5126
AclWI GGATC 9 cut(s) 535, 882, 1360, 2290, 2322, 2684, 2746, 3059, 4935
AcoI YGGCCR 1 cut(s) 3073
AcuI CTGAAG 5 cut(s) 1496, 1730, 3285, 3474, 5142
AfaI GTAC 8 cut(s) 605, 836, 1737, 2062, 3016, 3727, 3918, 4967
AfeI AGCGCT 1 cut(s) 507
AflII CTTAAG 1 cut(s) 1216
AflIII ACRYGT 3 cut(s) 216, 2403, 2598
AjiI CACGTC 1 cut(s) 4583
AjnI CCWGG 9 cut(s) 820, 1248, 2098, 2125, 2548, 3547, 4105, 4207, 4532
AleI CACNNNNGTG 1 cut(s) 901
Alw21I GWGCWC 6 cut(s) 1561, 2246, 2416, 2671, 3891, 4593
Alw26I GTCTC 4 cut(s) 969, 2390, 2550, 3648
Alw44I GTGCAC 3 cut(s) 2242, 2667, 3887
AlwI GGATC 9 cut(s) 535, 882, 1360, 2290, 2322, 2684, 2746, 3059, 4935
AlwNI CAGNNNCTG 2 cut(s) 1013, 1046
Ama87I CYCGRG 1 cut(s) 121
Aor13HI TCCGGA 2 cut(s) 325, 4894
Aor51HI AGCGCT 1 cut(s) 507
AoxI GGCC 9 cut(s) 818, 1491, 2201, 3055, 3073, 4253, 4319, 4751, 4762
ApaLI GTGCAC 3 cut(s) 2242, 2667, 3887
ArsI GACNNNNNNTTYG 2 cut(s) 3344, 3376
Asp700I GAANNNNTTC 3 cut(s) 2479, 3675, 4086
AspLEI GCGC 4 cut(s) 508, 2631, 3104, 4821
AsuC2I CCSGG 5 cut(s) 3077, 4708, 4904, 4976, 5045
AvaI CYCGRG 1 cut(s) 121
BaeGI GKGCMC 4 cut(s) 833, 2246, 2671, 3891
BanI GGYRCC 2 cut(s) 847, 2434
BanII GRGCYC 3 cut(s) 1561, 3396, 4593
BbsI GAAGAC 1 cut(s) 3384
Bbv12I GWGCWC 6 cut(s) 1561, 2246, 2416, 2671, 3891, 4593
BceAI ACGGC 2 cut(s) 2589, 4334
BciT130I CCWGG 9 cut(s) 822, 1250, 2100, 2127, 2550, 3549, 4107, 4209, 4534
BciVI GTATCC 2 cut(s) 1797, 4888
BclI TGATCA 3 cut(s) 925, 1897, 2272
BcnI CCSGG 5 cut(s) 3077, 4708, 4904, 4976, 5045
BcoDI GTCTC 4 cut(s) 969, 2390, 2550, 3648
BfaI CTAG 9 cut(s) 866, 1131, 1223, 1820, 1946, 2015, 2180, 3948, 5155
BfmI CTRYAG 3 cut(s) 1694, 2972, 4308
BfoI RGCGCY 2 cut(s) 509, 2632
BfrI CTTAAG 1 cut(s) 1216
BfuAI ACCTGC 1 cut(s) 1690
BfuI GTATCC 2 cut(s) 1797, 4888
BglII AGATCT 3 cut(s) 862, 3877, 3904
BmeT110I CYCGRG 1 cut(s) 121
BmgBI CACGTC 1 cut(s) 4583
BmrI ACTGGG 2 cut(s) 1542, 4164
BmsI GCATC 7 cut(s) 145, 238, 1288, 3310, 3726, 4653, 4722
BmuI ACTGGG 2 cut(s) 1542, 4164
BpiI GAAGAC 1 cut(s) 3384
BplI GAGNNNNNCTC 4 cut(s) 782, 814, 3245, 3277
BpmI CTGGAG 8 cut(s) 2148, 3717, 3885, 3929, 4128, 4230, 4308, 4622
Bpu10I CCTNAGC 1 cut(s) 3082
BpuEI CTTGAG 3 cut(s) 1138, 1821, 2414
BpuMI CCSGG 5 cut(s) 3077, 4708, 4904, 4976, 5045
BsaAI YACGTR 2 cut(s) 834, 2666
BsaBI GATNNNNATC 1 cut(s) 873
BsaJI CCNNGG 8 cut(s) 183, 233, 633, 2081, 2984, 3058, 3076, 4030
BsaWI WCCGGW 2 cut(s) 325, 4894
Bse118I RCCGGY 2 cut(s) 1125, 3471
Bse1I ACTGG 8 cut(s) 512, 691, 1548, 3160, 3700, 3794, 4159, 4874
Bse3DI GCAATG 3 cut(s) 1531, 2916, 4298
Bse8I GATNNNNATC 1 cut(s) 873
BseAI TCCGGA 2 cut(s) 325, 4894
BseBI CCWGG 9 cut(s) 822, 1250, 2100, 2127, 2550, 3549, 4107, 4209, 4534
BseDI CCNNGG 8 cut(s) 183, 233, 633, 2081, 2984, 3058, 3076, 4030
BseJI GATNNNNATC 1 cut(s) 873
BseMI GCAATG 3 cut(s) 1531, 2916, 4298
BseNI ACTGG 8 cut(s) 512, 691, 1548, 3160, 3700, 3794, 4159, 4874
BseRI GAGGAG 5 cut(s) 80, 200, 362, 461, 4514
BseSI GKGCMC 4 cut(s) 833, 2246, 2671, 3891
BseYI CCCAGC 2 cut(s) 502, 3296
BsgI GTGCAG 3 cut(s) 159, 1668, 4605
Bsh1236I CGCG 1 cut(s) 3102
BshFI GGCC 9 cut(s) 820, 1493, 2203, 3057, 3075, 4255, 4321, 4753, 4764
BshNI GGYRCC 2 cut(s) 847, 2434
BsiHKAI GWGCWC 6 cut(s) 1561, 2246, 2416, 2671, 3891, 4593
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 9 cut(s) 326, 1126, 3076, 3472, 4708, 4895, 4903, 4975, 5045
BslFI GGGAC 6 cut(s) 10, 971, 1063, 1391, 2621, 4919
BsmAI GTCTC 4 cut(s) 969, 2390, 2550, 3648
BsmFI GGGAC 6 cut(s) 10, 971, 1063, 1391, 2621, 4919
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 9 cut(s) 820, 1493, 2203, 3057, 3075, 4255, 4321, 4753, 4764
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 8 cut(s) 833, 1561, 2246, 2416, 2671, 3396, 3891, 4593
Bsp13I TCCGGA 2 cut(s) 325, 4894
Bsp1407I TGTACA 2 cut(s) 1735, 3014
Bsp19I CCATGG 2 cut(s) 633, 2984
BspACI CCGC 6 cut(s) 251, 845, 2035, 2265, 4790, 5126
BspANI GGCC 9 cut(s) 820, 1493, 2203, 3057, 3075, 4255, 4321, 4753, 4764
BspEI TCCGGA 2 cut(s) 325, 4894
BspFNI CGCG 1 cut(s) 3102
BspHI TCATGA 2 cut(s) 1900, 2532
BspMI ACCTGC 1 cut(s) 1690
BspPI GGATC 9 cut(s) 535, 882, 1360, 2290, 2322, 2684, 2746, 3059, 4935
BspQI GCTCTTC 2 cut(s) 2619, 2756
BspT107I GGYRCC 2 cut(s) 847, 2434
BspTI CTTAAG 1 cut(s) 1216
BsrDI GCAATG 3 cut(s) 1531, 2916, 4298
BsrFI RCCGGY 2 cut(s) 1125, 3471
BsrGI TGTACA 2 cut(s) 1735, 3014
BsrI ACTGG 8 cut(s) 512, 691, 1548, 3160, 3700, 3794, 4159, 4874
BssAI RCCGGY 2 cut(s) 1125, 3471
BssECI CCNNGG 8 cut(s) 183, 233, 633, 2081, 2984, 3058, 3076, 4030
BssT1I CCWWGG 6 cut(s) 233, 633, 2081, 2984, 3058, 4030
Bst2UI CCWGG 9 cut(s) 822, 1250, 2100, 2127, 2550, 3549, 4107, 4209, 4534
Bst4CI ACNGT 6 cut(s) 1912, 1969, 3149, 3997, 4336, 4770
Bst6I CTCTTC 5 cut(s) 651, 660, 2619, 2756, 2811
BstAFI CTTAAG 1 cut(s) 1216
BstAUI TGTACA 2 cut(s) 1735, 3014
BstBAI YACGTR 2 cut(s) 834, 2666
BstC8I GCNNGC 4 cut(s) 1319, 2840, 2844, 5134
BstDSI CCRYGG 2 cut(s) 633, 2984
BstEII GGTNACC 1 cut(s) 3350
BstENI CCTNNNNNAGG 3 cut(s) 149, 1485, 1836
BstFNI CGCG 1 cut(s) 3102
BstH2I RGCGCY 2 cut(s) 509, 2632
BstHHI GCGC 4 cut(s) 508, 2631, 3104, 4821
BstMAI GTCTC 4 cut(s) 969, 2390, 2550, 3648
BstMWI GCNNNNNNNGC 7 cut(s) 284, 817, 2086, 2584, 3455, 3458, 5015
BstNI CCWGG 9 cut(s) 822, 1250, 2100, 2127, 2550, 3549, 4107, 4209, 4534
BstNSI RCATGY 9 cut(s) 220, 1390, 2407, 2578, 2602, 2842, 2955, 3825, 4444
BstPI GGTNACC 1 cut(s) 3350
BstSFI CTRYAG 3 cut(s) 1694, 2972, 4308
BstSLI GKGCMC 4 cut(s) 833, 2246, 2671, 3891
BstUI CGCG 1 cut(s) 3102
BstV2I GAAGAC 1 cut(s) 3384
BstX2I RGATCY 7 cut(s) 862, 874, 2282, 2314, 3877, 3904, 4927
BstXI CCANNNNNNTGG 2 cut(s) 1256, 3133
BstYI RGATCY 7 cut(s) 862, 874, 2282, 2314, 3877, 3904, 4927
BsuI GTATCC 2 cut(s) 1797, 4888
BsuRI GGCC 9 cut(s) 820, 1493, 2203, 3057, 3075, 4255, 4321, 4753, 4764
BtgI CCRYGG 2 cut(s) 633, 2984
BtgZI GCGATG 1 cut(s) 261
BtrI CACGTC 1 cut(s) 4583
BtsI GCAGTG 3 cut(s) 1647, 3138, 3247
BtsIMutI CAGTG 5 cut(s) 228, 1647, 1974, 3138, 3247
BveI ACCTGC 1 cut(s) 1690
Cac8I GCNNGC 4 cut(s) 1319, 2840, 2844, 5134
CaiI CAGNNNCTG 2 cut(s) 1013, 1046
CciI TCATGA 2 cut(s) 1900, 2532
CfoI GCGC 4 cut(s) 508, 2631, 3104, 4821
Cfr10I RCCGGY 2 cut(s) 1125, 3471
CseI GACGC 3 cut(s) 1653, 2108, 2499
Csp6I GTAC 8 cut(s) 604, 835, 1736, 2061, 3015, 3726, 3917, 4966
CviQI GTAC 8 cut(s) 604, 835, 1736, 2061, 3015, 3726, 3917, 4966
DraI TTTAAA 2 cut(s) 4869, 5076
EaeI YGGCCR 1 cut(s) 3073
Eam1104I CTCTTC 5 cut(s) 651, 660, 2619, 2756, 2811
EarI CTCTTC 5 cut(s) 651, 660, 2619, 2756, 2811
EciI GGCGGA 2 cut(s) 2050, 4805
Ecl136II GAGCTC 2 cut(s) 1559, 4591
Eco130I CCWWGG 6 cut(s) 233, 633, 2081, 2984, 3058, 4030
Eco147I AGGCCT 1 cut(s) 2203
Eco24I GRGCYC 3 cut(s) 1561, 3396, 4593
Eco32I GATATC 2 cut(s) 5050, 5152
Eco47III AGCGCT 1 cut(s) 507
Eco53kI GAGCTC 2 cut(s) 1559, 4591
Eco57I CTGAAG 5 cut(s) 1496, 1730, 3285, 3474, 5142
Eco88I CYCGRG 1 cut(s) 121
Eco91I GGTNACC 1 cut(s) 3350
EcoICRI GAGCTC 2 cut(s) 1559, 4591
EcoNI CCTNNNNNAGG 3 cut(s) 149, 1485, 1836
EcoO109I RGGNCCY 4 cut(s) 24, 1010, 1169, 1378
EcoO65I GGTNACC 1 cut(s) 3350
EcoRI GAATTC 2 cut(s) 1633, 3577
EcoRII CCWGG 9 cut(s) 820, 1248, 2098, 2125, 2548, 3547, 4105, 4207, 4532
EcoRV GATATC 2 cut(s) 5050, 5152
EcoT14I CCWWGG 6 cut(s) 233, 633, 2081, 2984, 3058, 4030
EcoT22I ATGCAT 4 cut(s) 524, 627, 3741, 4668
EcoT38I GRGCYC 3 cut(s) 1561, 3396, 4593
ErhI CCWWGG 6 cut(s) 233, 633, 2081, 2984, 3058, 4030
FalI AAGNNNNNCTT 4 cut(s) 2835, 2867, 3553, 3585
FaqI GGGAC 6 cut(s) 10, 971, 1063, 1391, 2621, 4919
FauNDI CATATG 1 cut(s) 1373
FbaI TGATCA 3 cut(s) 925, 1897, 2272
FblI GTMKAC 2 cut(s) 2595, 4934
FriOI GRGCYC 3 cut(s) 1561, 3396, 4593
FspBI CTAG 9 cut(s) 866, 1131, 1223, 1820, 1946, 2015, 2180, 3948, 5155
GlaI GCGC 4 cut(s) 507, 2630, 3103, 4820
GsaI CCCAGC 2 cut(s) 506, 3300
GsuI CTGGAG 8 cut(s) 2148, 3717, 3885, 3929, 4128, 4230, 4308, 4622
HaeII RGCGCY 2 cut(s) 509, 2632
HaeIII GGCC 9 cut(s) 820, 1493, 2203, 3057, 3075, 4255, 4321, 4753, 4764
HapII CCGG 9 cut(s) 326, 1126, 3076, 3472, 4708, 4895, 4903, 4975, 5045
HgaI GACGC 3 cut(s) 1653, 2108, 2499
HhaI GCGC 4 cut(s) 508, 2631, 3104, 4821
Hin6I GCGC 4 cut(s) 506, 2629, 3102, 4819
HinP1I GCGC 4 cut(s) 506, 2629, 3102, 4819
HincII GTYRAC 1 cut(s) 4935
HindII GTYRAC 1 cut(s) 4935
HindIII AAGCTT 7 cut(s) 1283, 1319, 1406, 1776, 1886, 1932, 2477
HpaII CCGG 9 cut(s) 326, 1126, 3076, 3472, 4708, 4895, 4903, 4975, 5045
Hpy99I CGWCG 3 cut(s) 1799, 2930, 3386
HpyCH4III ACNGT 6 cut(s) 1912, 1969, 3149, 3997, 4336, 4770
HpyCH4IV ACGT 5 cut(s) 833, 2665, 3166, 3384, 4582
HpyF10VI GCNNNNNNNGC 7 cut(s) 284, 817, 2086, 2584, 3455, 3458, 5015
HpySE526I ACGT 5 cut(s) 833, 2665, 3166, 3384, 4582
HspAI GCGC 4 cut(s) 506, 2629, 3102, 4819
KflI GGGWCCC 2 cut(s) 24, 1378
Kpn2I TCCGGA 2 cut(s) 325, 4894
Ksp22I TGATCA 3 cut(s) 925, 1897, 2272
LguI GCTCTTC 2 cut(s) 2619, 2756
LweI GCATC 7 cut(s) 145, 238, 1288, 3310, 3726, 4653, 4722
MaeI CTAG 9 cut(s) 866, 1131, 1223, 1820, 1946, 2015, 2180, 3948, 5155
MaeII ACGT 5 cut(s) 833, 2665, 3166, 3384, 4582
MfeI CAATTG 1 cut(s) 3648
MflI RGATCY 7 cut(s) 862, 874, 2282, 2314, 3877, 3904, 4927
MhlI GDGCHC 8 cut(s) 833, 1561, 2246, 2416, 2671, 3396, 3891, 4593
MlyI GAGTC 8 cut(s) 113, 1085, 1097, 2601, 2923, 2974, 3254, 4697
MmeI TCCRAC 2 cut(s) 1119, 1818
Mph1103I ATGCAT 4 cut(s) 524, 627, 3741, 4668
MroI TCCGGA 2 cut(s) 325, 4894
MroXI GAANNNNTTC 3 cut(s) 2479, 3675, 4086
MslI CAYNNNNRTG 3 cut(s) 209, 221, 901
MspA1I CMGCKG 2 cut(s) 3452, 3461
MspCI CTTAAG 1 cut(s) 1216
MspI CCGG 9 cut(s) 326, 1126, 3076, 3472, 4708, 4895, 4903, 4975, 5045
MunI CAATTG 1 cut(s) 3648
Mva1269I GAATGC 1 cut(s) 289
MvaI CCWGG 9 cut(s) 822, 1250, 2100, 2127, 2550, 3549, 4107, 4209, 4534
MvnI CGCG 1 cut(s) 3102
MwoI GCNNNNNNNGC 7 cut(s) 284, 817, 2086, 2584, 3455, 3458, 5015
NciI CCSGG 5 cut(s) 3077, 4708, 4904, 4976, 5045
NcoI CCATGG 2 cut(s) 633, 2984
NdeI CATATG 1 cut(s) 1373
NmeAIII GCCGAG 1 cut(s) 390
NmuCI GTSAC 7 cut(s) 977, 2954, 3257, 3350, 3368, 4287, 5084
NsiI ATGCAT 4 cut(s) 524, 627, 3741, 4668
NspI RCATGY 9 cut(s) 220, 1390, 2407, 2578, 2602, 2842, 2955, 3825, 4444
OliI CACNNNNGTG 1 cut(s) 901
PaeI GCATGC 1 cut(s) 2842
PagI TCATGA 2 cut(s) 1900, 2532
PceI AGGCCT 1 cut(s) 2203
PciI ACATGT 3 cut(s) 216, 2403, 2598
PciSI GCTCTTC 2 cut(s) 2619, 2756
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 3 cut(s) 2479, 3675, 4086
PfoI TCCNGGA 4 cut(s) 2125, 2548, 4105, 4706
PleI GAGTC 8 cut(s) 113, 1084, 1096, 2600, 2922, 2974, 3254, 4697
PpsI GAGTC 8 cut(s) 113, 1084, 1096, 2600, 2922, 2974, 3254, 4697
Ppu21I YACGTR 2 cut(s) 834, 2666
PpuMI RGGWCCY 4 cut(s) 24, 1010, 1169, 1378
PscI ACATGT 3 cut(s) 216, 2403, 2598
PsiI TTATAA 2 cut(s) 1859, 1983
Psp124BI GAGCTC 2 cut(s) 1561, 4593
Psp5II RGGWCCY 4 cut(s) 24, 1010, 1169, 1378
Psp6I CCWGG 9 cut(s) 820, 1248, 2098, 2125, 2548, 3547, 4105, 4207, 4532
PspEI GGTNACC 1 cut(s) 3350
PspFI CCCAGC 2 cut(s) 502, 3296
PspGI CCWGG 9 cut(s) 820, 1248, 2098, 2125, 2548, 3547, 4105, 4207, 4532
PspPPI RGGWCCY 4 cut(s) 24, 1010, 1169, 1378
PsrI GAACNNNNNNTAC 2 cut(s) 3151, 3183
PstNI CAGNNNCTG 2 cut(s) 1013, 1046
PsuI RGATCY 7 cut(s) 862, 874, 2282, 2314, 3877, 3904, 4927
PvuII CAGCTG 2 cut(s) 3452, 3461
RsaI GTAC 8 cut(s) 605, 836, 1737, 2062, 3016, 3727, 3918, 4967
RsaNI GTAC 8 cut(s) 604, 835, 1736, 2061, 3015, 3726, 3917, 4966
RseI CAYNNNNRTG 3 cut(s) 209, 221, 901
SacI GAGCTC 2 cut(s) 1561, 4593
SalI GTCGAC 1 cut(s) 4933
SapI GCTCTTC 2 cut(s) 2619, 2756
SchI GAGTC 8 cut(s) 113, 1085, 1097, 2601, 2923, 2974, 3254, 4697
SduI GDGCHC 8 cut(s) 833, 1561, 2246, 2416, 2671, 3396, 3891, 4593
SfaNI GCATC 7 cut(s) 145, 238, 1288, 3310, 3726, 4653, 4722
SfcI CTRYAG 3 cut(s) 1694, 2972, 4308
SmiMI CAYNNNNRTG 3 cut(s) 209, 221, 901
SmlI CTYRAG 4 cut(s) 1153, 1216, 1836, 2393
SmoI CTYRAG 4 cut(s) 1153, 1216, 1836, 2393
SphI GCATGC 1 cut(s) 2842
SseBI AGGCCT 1 cut(s) 2203
SsiI CCGC 6 cut(s) 251, 845, 2035, 2265, 4790, 5126
SspI AATATT 3 cut(s) 1066, 2221, 3034
SspMI CTAG 9 cut(s) 866, 1131, 1223, 1820, 1946, 2015, 2180, 3948, 5155
SstI GAGCTC 2 cut(s) 1561, 4593
StuI AGGCCT 1 cut(s) 2203
StyI CCWWGG 6 cut(s) 233, 633, 2081, 2984, 3058, 4030
TaaI ACNGT 6 cut(s) 1912, 1969, 3149, 3997, 4336, 4770
TaiI ACGT 5 cut(s) 836, 2668, 3169, 3387, 4585
TaqI TCGA 9 cut(s) 409, 1231, 1275, 1631, 1768, 2590, 2961, 4843, 4934
TatI WGTACW 4 cut(s) 1735, 3014, 3916, 4965
TauI GCSGC 2 cut(s) 848, 2267
TscAI CASTG 5 cut(s) 228, 1654, 1974, 3138, 3247
TseFI GTSAC 7 cut(s) 977, 2954, 3257, 3350, 3368, 4287, 5084
Tsp45I GTSAC 7 cut(s) 977, 2954, 3257, 3350, 3368, 4287, 5084
TspGWI ACGGA 5 cut(s) 221, 659, 2048, 2603, 2887
TspRI CASTG 5 cut(s) 228, 1654, 1974, 3138, 3247
Vha464I CTTAAG 1 cut(s) 1216
VneI GTGCAC 3 cut(s) 2242, 2667, 3887
XagI CCTNNNNNAGG 3 cut(s) 149, 1485, 1836
XbaI TCTAGA 1 cut(s) 865
XceI RCATGY 9 cut(s) 220, 1390, 2407, 2578, 2602, 2842, 2955, 3825, 4444
XcmI CCANNNNNNNNNTGG 1 cut(s) 1779
XmiI GTMKAC 2 cut(s) 2595, 4934
XmnI GAANNNNTTC 3 cut(s) 2479, 3675, 4086
XspI CTAG 9 cut(s) 866, 1131, 1223, 1820, 1946, 2015, 2180, 3948, 5155
Zsp2I ATGCAT 4 cut(s) 524, 627, 3741, 4668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.