MD04G1086000.v1.1

Acid phosphatase homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005864
Physical Location & Seq
Forward (+)
12452832 .. 12457438
4607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1086000.v1.1.491

Sequence Viewer

Length: 792 bp
ATGGAGGCAGCAGCACCACCACCAGCGAAACATACCACCACCACAGCGCCACCAATCCTCGGCAGCGTAGTAGCTCTAGATACGACCGTCTCCCGACATCTCCACATCCTCGCCAAACCCTTCCTCCCCTATTCCCTCCTCCTCCTCCTCGAACTCTCCGCCGACTTCCGCTTCTTCTTCCCTCTCTCTCTCTCCCTCCTCCTTGCCCCAACCCTAACCCTAACCCCTATCCCGATCCCATCCCCAAGCCCAACCCAAATCCTCCGTCCACTCCTCTCCCCACTCATCCTCGGCCTCCTCCTCGACCTCGCCGTCGTCGGCCTAATCAAACTCCTCTTCCGCCGCTCCCGCCCTCTCTACAACAAGAACATGAGCGTCGCCGTTTCGGTGGACCATTTCTCCTTCCCCAGTGGGCACGCCTCTCGGGTTTGCTTCGTTGCCTCCCTCTTACACCTCTCCGCCGTCGCCCTCGCCGATGCCCTCGCCAACCTACGGTCATCGTCTCCCTTCGTTGATCGTTGGATCGGCACGGACCAGGTCAATGCCGTGAGTATTTTGGTCTCGGTTGCTTGGGCCTGGGCCGCCGGCACCTCCGTCTCTAGGGTTTTGCTGGGCAGGCATTTCGTCACCGACGTTTTCGCCGGGGCGTGTTTGGGAGTGCTTGAGGCTCTCGTTTCGTTTCACTTCCTGAGGACGGTTCCAGACTGGAGCAACCTCGAAGGCAATGTGGTGAAAATACTTTGTCAAGGTTTGGGATACTCCTTTTCATTTCTGATGACCCCCGTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP2

Protein Analysis

264

Amino Acids

28.34

Weight (kDa)

8.93

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 94 - 231 1.3e-18 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 587
AccBSI CCGCTC 1 cut(s) 345
AciI CCGC 7 cut(s) 159, 169, 340, 343, 349, 459, 582
AclWI GGATC 2 cut(s) 229, 530
AfiI CCNNNNNNNGG 4 cut(s) 59, 492, 600, 694
AjnI CCWGG 2 cut(s) 534, 575
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw26I GTCTC 4 cut(s) 94, 507, 565, 601
AlwI GGATC 2 cut(s) 229, 530
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 4 cut(s) 292, 319, 573, 579
ApeKI GCWGC 3 cut(s) 8, 11, 63
ArsI GACNNNNNNTTYG 2 cut(s) 250, 282
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 4 cut(s) 391, 532, 573, 579
AsuC2I CCSGG 1 cut(s) 643
AsuHPI GGTGA 2 cut(s) 619, 742
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 2 cut(s) 391, 532
AxyI CCTNAGG 1 cut(s) 689
BaeGI GKGCMC 1 cut(s) 417
BaeI ACNNNNGTAYC 2 cut(s) 72, 105
BanI GGYRCC 1 cut(s) 587
BbvI GCAGC 3 cut(s) 20, 23, 75
BccI CCATC 1 cut(s) 247
BceAI ACGGC 4 cut(s) 296, 365, 446, 530
BcgI CGANNNNNNTGC 4 cut(s) 404, 438, 604, 638
BciT130I CCWGG 2 cut(s) 536, 577
BciVI GTATCC 1 cut(s) 749
BcnI CCSGG 1 cut(s) 643
BcoDI GTCTC 4 cut(s) 94, 507, 565, 601
BfaI CTAG 2 cut(s) 77, 600
BfoI RGCGCY 1 cut(s) 50
BfuI GTATCC 1 cut(s) 749
BisI GCNGC 5 cut(s) 9, 12, 64, 343, 582
BlsI GCNGC 5 cut(s) 10, 13, 65, 344, 583
Bme1390I CCNGG 3 cut(s) 536, 577, 643
Bme18I GGWCC 2 cut(s) 391, 532
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 4 cut(s) 391, 532, 573, 579
BmiI GGNNCC 2 cut(s) 589, 699
BmrFI CCNGG 3 cut(s) 536, 577, 643
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 466
BmuI ACTGGG 1 cut(s) 402
BpmI CTGGAG 1 cut(s) 727
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 643
BsaI GGTCTC 1 cut(s) 565
BsaJI CCNNGG 4 cut(s) 58, 289, 576, 642
BsaXI ACNNNNNCTCC 4 cut(s) 108, 138, 383, 413
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 492, 600, 694
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 2 cut(s) 408, 710
Bse21I CCTNAGG 1 cut(s) 689
Bse3DI GCAATG 1 cut(s) 730
BseBI CCWGG 2 cut(s) 536, 577
BseDI CCNNGG 4 cut(s) 58, 289, 576, 642
BseGI GGATG 3 cut(s) 105, 239, 285
BseLI CCNNNNNNNGG 4 cut(s) 59, 492, 600, 694
BseMI GCAATG 1 cut(s) 730
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 2 cut(s) 408, 710
BseRI GAGGAG 9 cut(s) 128, 131, 134, 137, 188, 263, 287, 290, 323
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 3 cut(s) 20, 23, 75
BseYI CCCAGC 1 cut(s) 610
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 294, 321, 575, 581
BshNI GGYRCC 1 cut(s) 587
BsiEI CGRYCG 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 2 cut(s) 585, 642
BslI CCNNNNNNNGG 4 cut(s) 59, 492, 600, 694
BsmAI GTCTC 4 cut(s) 94, 507, 565, 601
BsmBI CGTCTC 3 cut(s) 94, 507, 601
BsnI GGCC 4 cut(s) 294, 321, 575, 581
Bso31I GGTCTC 1 cut(s) 565
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 3 cut(s) 234, 514, 522
BspACI CCGC 7 cut(s) 159, 169, 340, 343, 349, 459, 582
BspANI GGCC 4 cut(s) 294, 321, 575, 581
BspCNI CTCAG 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 589, 699
BspPI GGATC 2 cut(s) 229, 530
BspT107I GGYRCC 1 cut(s) 587
BspTNI GGTCTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 584
BsrI ACTGG 2 cut(s) 408, 710
BssAI RCCGGY 1 cut(s) 584
BssECI CCNNGG 4 cut(s) 58, 289, 576, 642
BssMI GATC 3 cut(s) 234, 514, 522
Bst2UI CCWGG 2 cut(s) 536, 577
Bst4CI ACNGT 3 cut(s) 88, 495, 697
Bst6I CTCTTC 1 cut(s) 341
BstC8I GCNNGC 3 cut(s) 417, 586, 617
BstDEI CTNAG 1 cut(s) 689
BstF5I GGATG 3 cut(s) 105, 239, 285
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 3 cut(s) 237, 517, 525
BstMAI GTCTC 4 cut(s) 94, 507, 565, 601
BstMBI GATC 3 cut(s) 234, 514, 522
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 3 cut(s) 348, 581, 616
BstNI CCWGG 2 cut(s) 536, 577
BstSCI CCNGG 3 cut(s) 534, 575, 641
BstSLI GKGCMC 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 20, 23, 75
Bsu36I CCTNAGG 1 cut(s) 689
BsuI GTATCC 1 cut(s) 749
BsuRI GGCC 4 cut(s) 294, 321, 575, 581
BtsCI GGATG 3 cut(s) 105, 239, 285
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 3 cut(s) 417, 586, 617
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 584
Cfr13I GGNCC 4 cut(s) 391, 532, 573, 579
CseI GACGC 1 cut(s) 364
CsiI ACCWGGT 1 cut(s) 534
CviAII CATG 1 cut(s) 370
CviJI RGCY 7 cut(s) 74, 249, 294, 321, 575, 581, 668
CviKI_1 RGCY 7 cut(s) 74, 249, 294, 321, 575, 581, 668
DdeI CTNAG 1 cut(s) 689
DpnI GATC 3 cut(s) 236, 516, 524
DpnII GATC 3 cut(s) 234, 514, 522
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
Eam1104I CTCTTC 1 cut(s) 341
EarI CTCTTC 1 cut(s) 341
EciI GGCGGA 3 cut(s) 148, 329, 448
Eco31I GGTCTC 1 cut(s) 565
Eco47I GGWCC 2 cut(s) 391, 532
Eco81I CCTNAGG 1 cut(s) 689
Eco88I CYCGRG 1 cut(s) 423
EcoRII CCWGG 2 cut(s) 534, 575
Esp3I CGTCTC 3 cut(s) 94, 507, 601
FaeI CATG 1 cut(s) 373
FaiI YATR 2 cut(s) 33, 371
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 356
Fnu4HI GCNGC 5 cut(s) 9, 12, 64, 343, 582
FokI GGATG 3 cut(s) 92, 226, 272
Fsp4HI GCNGC 5 cut(s) 9, 12, 64, 343, 582
FspBI CTAG 2 cut(s) 77, 600
GlaI GCGC 1 cut(s) 48
GluI GCNGC 5 cut(s) 9, 12, 64, 343, 582
GsaI CCCAGC 1 cut(s) 614
GsuI CTGGAG 1 cut(s) 727
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 4 cut(s) 294, 321, 575, 581
HapII CCGG 2 cut(s) 585, 642
HgaI GACGC 1 cut(s) 364
HhaI GCGC 1 cut(s) 49
Hin1II CATG 1 cut(s) 373
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HpaII CCGG 2 cut(s) 585, 642
HphI GGTGA 2 cut(s) 619, 742
Hpy166II GTNNAC 2 cut(s) 269, 391
Hpy188I TCNGA 1 cut(s) 774
Hpy188III TCNNGA 5 cut(s) 77, 93, 232, 688, 701
Hpy8I GTNNAC 2 cut(s) 269, 391
Hpy99I CGWCG 5 cut(s) 317, 320, 380, 467, 635
HpyAV CCTTC 4 cut(s) 130, 412, 517, 713
HpyCH4III ACNGT 3 cut(s) 88, 495, 697
HpyCH4IV ACGT 1 cut(s) 633
HpyF10VI GCNNNNNNNGC 3 cut(s) 348, 581, 616
HpyF3I CTNAG 1 cut(s) 689
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 1 cut(s) 373
HspAI GCGC 1 cut(s) 47
KroI GCCGGC 1 cut(s) 584
KroNI GCCGGC 1 cut(s) 586
Kzo9I GATC 3 cut(s) 234, 514, 522
LmnI GCTCC 2 cut(s) 350, 708
Lsp1109I GCAGC 3 cut(s) 20, 23, 75
LweI GCATC 1 cut(s) 466
MabI ACCWGGT 1 cut(s) 534
MaeI CTAG 2 cut(s) 77, 600
MaeII ACGT 1 cut(s) 633
MaeIII GTNAC 1 cut(s) 625
MalI GATC 3 cut(s) 236, 516, 524
MbiI CCGCTC 1 cut(s) 345
MboI GATC 3 cut(s) 234, 514, 522
MboII GAAGA 3 cut(s) 166, 169, 328
MhlI GDGCHC 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 500
MroNI GCCGGC 1 cut(s) 584
MspI CCGG 2 cut(s) 585, 642
MspR9I CCNGG 3 cut(s) 536, 577, 643
MvaI CCWGG 2 cut(s) 536, 577
MwoI GCNNNNNNNGC 3 cut(s) 348, 581, 616
NaeI GCCGGC 1 cut(s) 586
NciI CCSGG 1 cut(s) 643
NdeII GATC 3 cut(s) 234, 514, 522
NgoMIV GCCGGC 1 cut(s) 584
NlaIII CATG 1 cut(s) 373
NlaIV GGNNCC 2 cut(s) 589, 699
NmeAIII GCCGAG 2 cut(s) 39, 270
NmuCI GTSAC 1 cut(s) 625
PcsI WCGNNNNNNNCGW 3 cut(s) 309, 471, 630
PdiI GCCGGC 1 cut(s) 586
PflFI GACNNNGTC 1 cut(s) 536
PkrI GCNGC 5 cut(s) 10, 13, 65, 344, 583
Psp6I CCWGG 2 cut(s) 534, 575
PspFI CCCAGC 1 cut(s) 610
PspGI CCWGG 2 cut(s) 534, 575
PspN4I GGNNCC 2 cut(s) 589, 699
PspPI GGNCC 4 cut(s) 391, 532, 573, 579
PsyI GACNNNGTC 1 cut(s) 536
SatI GCNGC 5 cut(s) 9, 12, 64, 343, 582
Sau3AI GATC 3 cut(s) 234, 514, 522
Sau96I GGNCC 4 cut(s) 391, 532, 573, 579
ScrFI CCNGG 3 cut(s) 536, 577, 643
SduI GDGCHC 1 cut(s) 417
SetI ASST 9 cut(s) 76, 309, 456, 492, 540, 593, 636, 717, 751
SexAI ACCWGGT 1 cut(s) 534
SfaNI GCATC 1 cut(s) 466
SinI GGWCC 2 cut(s) 391, 532
SmlI CTYRAG 1 cut(s) 662
SmoI CTYRAG 1 cut(s) 662
SsiI CCGC 7 cut(s) 159, 169, 340, 343, 349, 459, 582
SspMI CTAG 2 cut(s) 77, 600
StyD4I CCNGG 3 cut(s) 534, 575, 641
TaaI ACNGT 3 cut(s) 88, 495, 697
TaiI ACGT 1 cut(s) 636
TaqI TCGA 3 cut(s) 150, 303, 717
TauI GCSGC 2 cut(s) 345, 584
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 3 cut(s) 8, 11, 63
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 1 cut(s) 756
TspGWI ACGGA 3 cut(s) 254, 545, 583
TspRI CASTG 1 cut(s) 415
Tth111I GACNNNGTC 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 391, 532
XbaI TCTAGA 1 cut(s) 76
XspI CTAG 2 cut(s) 77, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.