MD06G1062400.v1.1

Acid phosphatase homologues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005864
Physical Location & Seq
Reverse (-)
10581245 .. 10584070
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1062400.v1.1.491

Sequence Viewer

Length: 627 bp
ATGCTCGGCCGCTTAGTAGCTCTAGATACGACCGTTTCCCAACATCTCTACGTCCTCGCCAAACCCTTCCTCCCCCACTCCCTTCTCCTCCTCCTCGAACTCTCAGCCGACTTCCGCTTCTTCTTCCCTCTCTCCCTCTCCCTCCTCCTTGCCCCGATCCCAGACCCAAGCCCAACCCAAATCCTCTGCCCACTCCTCTCCCCACTCATCTTCGGCCTCCTCCTCGACCTCGCCGTCGTCGGCCTAATCAAACTCCTCTTTCGCCGCTCCCGCCCTCTCTACAACAAGAAGATGAGCGTCGCCGTTTCGGTAGACCACTTCTCCTTCCCCAGCGGACACTCCTCTCCGGTTTGCTTCGTCGCCTCCCTCTTGCACCTCTCCGCTGCCGCCCTTACCGATGCCCTCGCCAACCTACGGTCAGCGTCTCCCTTCGTTGATCGTTGGATCGGCGCGGACGAGGTCAATGCCGTGAGGATTTTGGTCTCGGTTGCTTGGGCCTGGGCCGTCGGCACCTCCGTCTCTAGGGTTTTGCTGGGTAGGCATTTCGTCACCGAAGTTTTCACCGGGGCGTGTTTGGGCGTGCTTGAGGCTCTGGTTTCGTTTCACTTGCTGAGCTTCCGACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP2

Protein Analysis

209

Amino Acids

22.66

Weight (kDa)

8.99

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP2 PF01569 68 - 205 6.5e-17 PAP2 superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 509
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 312
AccII CGCG 1 cut(s) 452
AciI CCGC 8 cut(s) 10, 115, 265, 271, 333, 381, 387, 452
AclWI GGATC 2 cut(s) 151, 452
AcoI YGGCCR 1 cut(s) 7
AfiI CCNNNNNNNGG 2 cut(s) 414, 522
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 2 cut(s) 20, 615
AluI AGCT 2 cut(s) 20, 615
Alw26I GTCTC 3 cut(s) 429, 487, 523
AlwI GGATC 2 cut(s) 151, 452
AoxI GGCC 5 cut(s) 7, 214, 241, 495, 501
ApeKI GCWGC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 495, 501
AsuC2I CCSGG 1 cut(s) 565
AsuHPI GGTGA 2 cut(s) 541, 553
BaeI ACNNNNGTAYC 2 cut(s) 18, 51
BanI GGYRCC 1 cut(s) 509
BbvI GCAGC 1 cut(s) 370
BceAI ACGGC 4 cut(s) 218, 287, 452, 488
BcgI CGANNNNNNTGC 2 cut(s) 446, 480
BciT130I CCWGG 1 cut(s) 499
BcnI CCSGG 1 cut(s) 565
BcoDI GTCTC 3 cut(s) 429, 487, 523
BfaI CTAG 2 cut(s) 23, 522
BisI GCNGC 4 cut(s) 10, 265, 384, 387
BlpI GCTNAGC 1 cut(s) 611
BlsI GCNGC 4 cut(s) 11, 266, 385, 388
Bme1390I CCNGG 2 cut(s) 499, 565
BmgT120I GGNCC 2 cut(s) 495, 501
BmiI GGNNCC 1 cut(s) 511
BmrFI CCNGG 2 cut(s) 499, 565
BmsI GCATC 1 cut(s) 388
Bpu1102I GCTNAGC 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 565
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 498, 564
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 4 cut(s) 54, 84, 305, 335
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 522
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 2 cut(s) 498, 564
BseLI CCNNNNNNNGG 2 cut(s) 414, 522
BseMII CTCAG 2 cut(s) 117, 602
BseRI GAGGAG 9 cut(s) 77, 80, 83, 134, 185, 209, 212, 245, 331
BseX3I CGGCCG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 370
BseYI CCCAGC 2 cut(s) 329, 532
Bsh1236I CGCG 1 cut(s) 452
Bsh1285I CGRYCG 2 cut(s) 10, 33
BshFI GGCC 5 cut(s) 9, 216, 243, 497, 503
BshNI GGYRCC 1 cut(s) 509
BsiEI CGRYCG 2 cut(s) 10, 33
BsiSI CCGG 2 cut(s) 347, 564
BslI CCNNNNNNNGG 2 cut(s) 414, 522
BsmAI GTCTC 3 cut(s) 429, 487, 523
BsmBI CGTCTC 2 cut(s) 429, 523
BsnI GGCC 5 cut(s) 9, 216, 243, 497, 503
Bso31I GGTCTC 1 cut(s) 487
Bsp143I GATC 3 cut(s) 156, 436, 444
Bsp1720I GCTNAGC 1 cut(s) 611
BspACI CCGC 8 cut(s) 10, 115, 265, 271, 333, 381, 387, 452
BspANI GGCC 5 cut(s) 9, 216, 243, 497, 503
BspCNI CTCAG 2 cut(s) 116, 603
BspFNI CGCG 1 cut(s) 452
BspLI GGNNCC 1 cut(s) 511
BspPI GGATC 2 cut(s) 151, 452
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 498, 564
BssMI GATC 3 cut(s) 156, 436, 444
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 3 cut(s) 34, 417, 624
BstC8I GCNNGC 1 cut(s) 581
BstDEI CTNAG 3 cut(s) 13, 103, 611
BstFNI CGCG 1 cut(s) 452
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 3 cut(s) 159, 439, 447
BstMAI GTCTC 3 cut(s) 429, 487, 523
BstMBI GATC 3 cut(s) 156, 436, 444
BstMCI CGRYCG 2 cut(s) 10, 33
BstMWI GCNNNNNNNGC 2 cut(s) 270, 538
BstNI CCWGG 1 cut(s) 499
BstSCI CCNGG 2 cut(s) 497, 563
BstUI CGCG 1 cut(s) 452
BstV1I GCAGC 1 cut(s) 370
BstZI CGGCCG 1 cut(s) 7
BsuRI GGCC 5 cut(s) 9, 216, 243, 497, 503
Cac8I GCNNGC 1 cut(s) 581
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 2 cut(s) 495, 501
CseI GACGC 2 cut(s) 286, 411
DdeI CTNAG 3 cut(s) 13, 103, 611
DpnI GATC 3 cut(s) 158, 438, 446
DpnII GATC 3 cut(s) 156, 436, 444
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
EclXI CGGCCG 1 cut(s) 7
Eco31I GGTCTC 1 cut(s) 487
Eco52I CGGCCG 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 497
Esp3I CGTCTC 2 cut(s) 429, 523
FauI CCCGC 1 cut(s) 278
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 4 cut(s) 10, 265, 384, 387
Fsp4HI GCNGC 4 cut(s) 10, 265, 384, 387
FspBI CTAG 2 cut(s) 23, 522
GlaI GCGC 1 cut(s) 451
GluI GCNGC 4 cut(s) 10, 265, 384, 387
GsaI CCCAGC 2 cut(s) 333, 536
HaeIII GGCC 5 cut(s) 9, 216, 243, 497, 503
HapII CCGG 2 cut(s) 347, 564
HgaI GACGC 2 cut(s) 286, 411
HhaI GCGC 1 cut(s) 452
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HpaII CCGG 2 cut(s) 347, 564
HphI GGTGA 2 cut(s) 541, 553
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 1 cut(s) 620
Hpy188III TCNNGA 1 cut(s) 23
Hpy8I GTNNAC 1 cut(s) 313
Hpy99I CGWCG 5 cut(s) 239, 242, 302, 362, 509
HpyAV CCTTC 4 cut(s) 76, 92, 334, 439
HpyCH4III ACNGT 3 cut(s) 34, 417, 624
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 1 cut(s) 373
HpyF10VI GCNNNNNNNGC 2 cut(s) 270, 538
HpyF3I CTNAG 3 cut(s) 13, 103, 611
HpySE526I ACGT 1 cut(s) 51
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 3 cut(s) 156, 436, 444
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 8 cut(s) 174, 343, 360, 484, 511, 518, 577, 578
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 1 cut(s) 388
MaeI CTAG 2 cut(s) 23, 522
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 1 cut(s) 547
MalI GATC 3 cut(s) 158, 438, 446
MbiI CCGCTC 1 cut(s) 267
MboI GATC 3 cut(s) 156, 436, 444
MboII GAAGA 4 cut(s) 112, 115, 202, 301
MmeI TCCRAC 1 cut(s) 422
MspA1I CMGCKG 2 cut(s) 333, 383
MspI CCGG 2 cut(s) 347, 564
MspR9I CCNGG 2 cut(s) 499, 565
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 1 cut(s) 452
MwoI GCNNNNNNNGC 2 cut(s) 270, 538
NciI CCSGG 1 cut(s) 565
NdeII GATC 3 cut(s) 156, 436, 444
NlaIV GGNNCC 1 cut(s) 511
NmuCI GTSAC 1 cut(s) 547
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 453, 513
PflFI GACNNNGTC 1 cut(s) 458
PkrI GCNGC 4 cut(s) 11, 266, 385, 388
Psp6I CCWGG 1 cut(s) 497
PspFI CCCAGC 2 cut(s) 329, 532
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 1 cut(s) 511
PspPI GGNCC 2 cut(s) 495, 501
PsyI GACNNNGTC 1 cut(s) 458
SatI GCNGC 4 cut(s) 10, 265, 384, 387
Sau3AI GATC 3 cut(s) 156, 436, 444
Sau96I GGNCC 2 cut(s) 495, 501
ScrFI CCNGG 2 cut(s) 499, 565
SetI ASST 8 cut(s) 22, 54, 231, 378, 414, 462, 515, 617
SfaNI GCATC 1 cut(s) 388
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SsiI CCGC 8 cut(s) 10, 115, 265, 271, 333, 381, 387, 452
SspMI CTAG 2 cut(s) 23, 522
StyD4I CCNGG 2 cut(s) 497, 563
TaaI ACNGT 3 cut(s) 34, 417, 624
TaiI ACGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 96, 225
TauI GCSGC 3 cut(s) 12, 267, 389
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 547
TspGWI ACGGA 1 cut(s) 505
Tth111I GACNNNGTC 1 cut(s) 458
XbaI TCTAGA 1 cut(s) 22
XmiI GTMKAC 1 cut(s) 312
XspI CTAG 2 cut(s) 23, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.