Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003976

Physical Location & Seq

Genomic Coordinates Reverse (-)

19821122 .. 19824002

Gene Structure 2881 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1112600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1401 bp

ATGGGTTGGATGATGATTCCCTGTTCTGGTAATTCCAACTGTAAAGCAAAAACCAAGAAGAAAAGGAAGAACAAGATTGAGTTACAGGATAAGCCACTTGATCAGATCAAACCCACTTCAGCCATGCAGGATTGTTTTCGGAAAGCTTGGCATCATCTGCAAAATCCAGCTCAGTCTTTATTACAATTTAGTTGCTATATTTATGAGAAGTTTATAGGTTTTTCAAAGACTAGTTCCATTTTGAGTGTCAAGGAGGATCCAAAAGATCGAGGGTCTGATCACATTGAAGCACAGACTTTTGTATTTCGTGAATTGGCAGCTGCAACTAGAAATTTCAGGGCAGAATGTCTTTTGGGTGAAGGTGGATTTGGTCGAGTATTCAAGGGACGTTTGGAAAGGACAAATCAGGTAGTGGCTATCAAACAACTTGATCGTGATGGATTACAAGGGAACAGGGAATTCCTTGTTGAATTGTTGATGTTTAGTCTTCTTCACCACCCCAATCTTGTTAATCTTATTGGTTATTGTGCGGATGGAGATCAAAGGCTTCTTGTTTATGAATATATGCCCCTAGGGTCATTGGGCGACCATTTACATGATGTGTTACCAGGTAAGAGACGACTTGATTGGAATACGCGAATGAAAATAGCCGCTGGTGCAGCGAAGGGATTGAAGTATCTACATGACAAAGCAAGTCCTCCTGTTCTACACAGAAATTTGAAATGCTCCAATATTTTGCTCGGTGAAGGGTATCATCCAAAGCTGTCTGGTTTTGGCTTAGCCAAACTTGGGCCTGTTGGGGATAACACCCATGTATCTAAATGGGTAATGGGAACGTATGGATATTGTGCTCCAGAGTATGCAATGACAGGGCAGTTGACTCTTAAATCAGACATTTATAGCTTTGGTGTAGTTCTTTTGGAAATTATAACAGGCAGGACAGCGATTGACAATACCAGAGGTGCAGGAGAACAGATTTTGGTTGAGTGGGCAAGAACCTTGTTTAAAGACCGAAGGAAACTTTCGCAAATGGCAGACCCAACGCTCCAGGGTCAGTATCCCAAAAGGGGGTTGTACCAAGCTCTTGCAGTTGCGGGAATGTGTGTTCAGGAGCAGCCTAATATGCGGCCAGTTATAGCTGATGTCGTCACAGCTTTGACTTACCTTGCTTCACAGAAGTATGACCATGAAACAGAGCCAGTCCAAAGCTCCCGTCTTGCCCCTTGTACTCCCCCTAGAACCAAGAGGGATATTGAAAAGGAAGCTCAATTGTGTGTTTCTCTTCTTTGTTTTTTCTTCTTGAATATATACAGAGCCGGAATGCTGTATTATATATTTGTGTGCCTTCGTGCAGTGATTTTGATTTGTATTTTTACATCTTTATTCTTCAAATATCTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0031347 GO:0031349 GO:0036211 GO:0038023 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1900457 GO:1900459 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

467

Amino Acids

52.65

Weight (kDa)

9.26

Isoelectric Point (pI)

37.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	116 - 385	9.2e-44	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	116 - 382	3.5e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02800
fragaria_vesca	FvH4_6g15040 FvH4_7g20570
malus_domestica	MD01G1114700.v1.1 MD04G1112600.v1.1 MD04G1113000.v1.1 MD07G1182700.v1.1 MD12G1129100.v1.1
prunus_persica	Prupe.2G221400_v2.0.a1 Prupe.2G221400_v2.0.a1 Prupe.6G244400_v2.0.a1 Prupe.6G244400_v2.0.a1
pyrus_communis	pycom01g14360 pycom04g10250 pycom04g10290 pycom12g12540
rosa_chinensis	RchiOBHm_Chr1g0364451 RchiOBHm_Chr3g0468211
rosa_laevigata	RLG00000024441 RLG00000027478
rosa_multiflora	Rmu_sc0001342.1_g000009 Rmu_sc0004524.1_g000006
rosa_roxburghii	Rroxscaffold_4G00291820 Rroxscaffold_4G00292260 Rroxscaffold_6G00412910
rosa_rugosa	Rorug01G0316100 Rorug03G0094200
rosa_samantha	Rh1AG324600 Rh1BG287300 Rh1DG318800 Rh3DG164000
rosa_wichuraiana	Rw1G028770 Rw3G013430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	929
AccII	CGCG	1 cut(s)	637
AciI	CCGC	4 cut(s)	530, 651, 1094, 1126
AclWI	GGATC	2 cut(s)	251, 264
AcoI	YGGCCR	1 cut(s)	1127
AcsI	RAATTY	3 cut(s)	331, 458, 715
AcuI	CTGAAG	1 cut(s)	102
AfaI	GTAC	2 cut(s)	1076, 1228
AfiI	CCNNNNNNNGG	4 cut(s)	26, 789, 1067, 1068
AgsI	TTSAA	9 cut(s)	225, 287, 382, 470, 673, 721, 1256, 1303, 1390
AhlI	ACTAGT	1 cut(s)	230
AjnI	CCWGG	2 cut(s)	607, 1047
AjuI	GAANNNNNNNTTGG	2 cut(s)	351, 383
AloI	GAACNNNNNNTCC	2 cut(s)	1089, 1121
Alw21I	GWGCWC	1 cut(s)	853
Alw26I	GTCTC	1 cut(s)	610
AlwI	GGATC	2 cut(s)	251, 264
AoxI	GGCC	2 cut(s)	791, 1127
ApeKI	GCWGC	4 cut(s)	317, 320, 659, 1114
ApoI	RAATTY	3 cut(s)	331, 458, 715
ArsI	GACNNNNNNTTYG	2 cut(s)	1198, 1230
AspA2I	CCTAGG	1 cut(s)	571
AspS9I	GGNCC	1 cut(s)	791
AsuHPI	GGTGA	3 cut(s)	368, 485, 755
AvrII	CCTAGG	1 cut(s)	571
BaeI	ACNNNNGTAYC	2 cut(s)	798, 831
BamHI	GGATCC	1 cut(s)	256
BbsI	GAAGAC	1 cut(s)	479
Bbv12I	GWGCWC	1 cut(s)	853
BbvI	GCAGC	4 cut(s)	307, 329, 671, 1126
BccI	CCATC	2 cut(s)	431, 527
BciT130I	CCWGG	2 cut(s)	609, 1049
BciVI	GTATCC	1 cut(s)	1068
BclI	TGATCA	2 cut(s)	100, 277
BcoDI	GTCTC	1 cut(s)	610
BcuI	ACTAGT	1 cut(s)	230
BfaI	CTAG	4 cut(s)	231, 327, 572, 1236
BfmI	CTRYAG	1 cut(s)	1397
BfuI	GTATCC	1 cut(s)	1068
BisI	GCNGC	6 cut(s)	318, 321, 651, 660, 1115, 1127
BlnI	CCTAGG	1 cut(s)	571
BlpI	GCTNAGC	1 cut(s)	778
BlsI	GCNGC	6 cut(s)	319, 322, 652, 661, 1116, 1128
Bme1390I	CCNGG	2 cut(s)	609, 1049
BmgT120I	GGNCC	1 cut(s)	791
BmiI	GGNNCC	1 cut(s)	258
BmrFI	CCNGG	2 cut(s)	609, 1049
BmsI	GCATC	1 cut(s)	160
BpiI	GAAGAC	1 cut(s)	479
BpmI	CTGGAG	2 cut(s)	837, 1031
Bpu1102I	GCTNAGC	1 cut(s)	778
BsaBI	GATNNNNATC	1 cut(s)	537
BsaJI	CCNNGG	2 cut(s)	571, 1048
Bsc4I	CCNNNNNNNGG	4 cut(s)	26, 789, 1067, 1068
Bse1I	ACTGG	2 cut(s)	1130, 1199
Bse3DI	GCAATG	1 cut(s)	870
Bse8I	GATNNNNATC	1 cut(s)	537
BseBI	CCWGG	2 cut(s)	609, 1049
BseDI	CCNNGG	2 cut(s)	571, 1048
BseGI	GGATG	3 cut(s)	15, 538, 754
BseJI	GATNNNNATC	1 cut(s)	537
BseLI	CCNNNNNNNGG	4 cut(s)	26, 789, 1067, 1068
BseMI	GCAATG	1 cut(s)	870
BseMII	CTCAG	1 cut(s)	185
BseNI	ACTGG	2 cut(s)	1130, 1199
BseXI	GCAGC	4 cut(s)	307, 329, 671, 1126
BsgI	GTGCAG	3 cut(s)	678, 984, 1371
Bsh1236I	CGCG	1 cut(s)	637
BshFI	GGCC	2 cut(s)	793, 1129
BsiHKAI	GWGCWC	1 cut(s)	853
BsiSI	CCGG	1 cut(s)	1317
BslFI	GGGAC	1 cut(s)	399
BslI	CCNNNNNNNGG	4 cut(s)	26, 789, 1067, 1068
BsmAI	GTCTC	1 cut(s)	610
BsmBI	CGTCTC	1 cut(s)	610
BsmFI	GGGAC	1 cut(s)	399
BsmI	GAATGC	1 cut(s)	1326
BsnI	GGCC	2 cut(s)	793, 1129
Bsp1286I	GDGCHC	1 cut(s)	853
Bsp143I	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
Bsp1720I	GCTNAGC	1 cut(s)	778
BspACI	CCGC	4 cut(s)	530, 651, 1094, 1126
BspANI	GGCC	2 cut(s)	793, 1129
BspCNI	CTCAG	1 cut(s)	184
BspFNI	CGCG	1 cut(s)	637
BspLI	GGNNCC	1 cut(s)	258
BspPI	GGATC	2 cut(s)	251, 264
BsrDI	GCAATG	1 cut(s)	870
BsrI	ACTGG	2 cut(s)	1130, 1199
BssECI	CCNNGG	2 cut(s)	571, 1048
BssMI	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
BssT1I	CCWWGG	1 cut(s)	571
Bst2UI	CCWGG	2 cut(s)	609, 1049
Bst4CI	ACNGT	1 cut(s)	41
Bst6I	CTCTTC	1 cut(s)	1287
BstAPI	GCANNNNNTGC	1 cut(s)	157
BstDEI	CTNAG	2 cut(s)	171, 778
BstF5I	GGATG	3 cut(s)	15, 538, 754
BstFNI	CGCG	1 cut(s)	637
BstKTI	GATC	7 cut(s)	103, 108, 259, 268, 280, 433, 541
BstMAI	GTCTC	1 cut(s)	610
BstMBI	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
BstMWI	GCNNNNNNNGC	4 cut(s)	157, 656, 659, 1123
BstNI	CCWGG	2 cut(s)	609, 1049
BstSCI	CCNGG	2 cut(s)	607, 1047
BstSFI	CTRYAG	1 cut(s)	1397
BstUI	CGCG	1 cut(s)	637
BstV1I	GCAGC	4 cut(s)	307, 329, 671, 1126
BstV2I	GAAGAC	1 cut(s)	479
BstX2I	RGATCY	1 cut(s)	256
BstYI	RGATCY	1 cut(s)	256
BsuI	GTATCC	1 cut(s)	1068
BsuRI	GGCC	2 cut(s)	793, 1129
BtsCI	GGATG	3 cut(s)	15, 538, 754
BtsI	GCAGTG	1 cut(s)	1359
BtsIMutI	CAGTG	1 cut(s)	1359
Cfr13I	GGNCC	1 cut(s)	791
CsiI	ACCWGGT	1 cut(s)	607
Csp6I	GTAC	2 cut(s)	1075, 1227
CviAII	CATG	5 cut(s)	124, 596, 683, 812, 1187
CviQI	GTAC	2 cut(s)	1075, 1227
DdeI	CTNAG	2 cut(s)	171, 778
DpnI	GATC	7 cut(s)	102, 107, 258, 267, 279, 432, 540
DpnII	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
DraI	TTTAAA	1 cut(s)	1006
EaeI	YGGCCR	1 cut(s)	1127
Eam1104I	CTCTTC	1 cut(s)	1287
EarI	CTCTTC	1 cut(s)	1287
Eco130I	CCWWGG	1 cut(s)	571
Eco57I	CTGAAG	1 cut(s)	102
EcoRI	GAATTC	1 cut(s)	458
EcoRII	CCWGG	2 cut(s)	607, 1047
EcoT14I	CCWWGG	1 cut(s)	571
ErhI	CCWWGG	1 cut(s)	571
Esp3I	CGTCTC	1 cut(s)	610
FaeI	CATG	5 cut(s)	127, 599, 686, 815, 1190
FaqI	GGGAC	1 cut(s)	399
FatI	CATG	5 cut(s)	123, 595, 682, 811, 1186
FauI	CCCGC	1 cut(s)	1087
FbaI	TGATCA	2 cut(s)	100, 277
Fnu4HI	GCNGC	6 cut(s)	318, 321, 651, 660, 1115, 1127
FokI	GGATG	3 cut(s)	22, 545, 741
Fsp4HI	GCNGC	6 cut(s)	318, 321, 651, 660, 1115, 1127
FspBI	CTAG	4 cut(s)	231, 327, 572, 1236
GluI	GCNGC	6 cut(s)	318, 321, 651, 660, 1115, 1127
GsuI	CTGGAG	2 cut(s)	837, 1031
HaeIII	GGCC	2 cut(s)	793, 1129
HapII	CCGG	1 cut(s)	1317
Hin1II	CATG	5 cut(s)	127, 599, 686, 815, 1190
HincII	GTYRAC	1 cut(s)	879
HindII	GTYRAC	1 cut(s)	879
HindIII	AAGCTT	1 cut(s)	144
HinfI	GANTC	2 cut(s)	16, 880
HpaII	CCGG	1 cut(s)	1317
HphI	GGTGA	3 cut(s)	368, 485, 755
Hpy166II	GTNNAC	1 cut(s)	879
Hpy188I	TCNGA	4 cut(s)	105, 141, 277, 892
Hpy188III	TCNNGA	5 cut(s)	308, 434, 854, 1109, 1300
Hpy8I	GTNNAC	1 cut(s)	879
HpyAV	CCTTC	5 cut(s)	353, 658, 740, 1008, 1355
HpyCH4III	ACNGT	1 cut(s)	41
HpyCH4IV	ACGT	2 cut(s)	388, 836
HpyCH4V	TGCA	8 cut(s)	127, 160, 323, 659, 863, 965, 1088, 1352
HpyF10VI	GCNNNNNNNGC	4 cut(s)	157, 656, 659, 1123
HpyF3I	CTNAG	2 cut(s)	171, 778
HpySE526I	ACGT	2 cut(s)	388, 836
Hsp92II	CATG	5 cut(s)	127, 599, 686, 815, 1190
Ksp22I	TGATCA	2 cut(s)	100, 277
Kzo9I	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
LmnI	GCTCC	5 cut(s)	731, 856, 1050, 1111, 1214
Lsp1109I	GCAGC	4 cut(s)	307, 329, 671, 1126
LweI	GCATC	1 cut(s)	160
MabI	ACCWGGT	1 cut(s)	607
MaeI	CTAG	4 cut(s)	231, 327, 572, 1236
MaeII	ACGT	2 cut(s)	388, 836
MaeIII	GTNAC	3 cut(s)	81, 603, 1147
MalI	GATC	7 cut(s)	102, 107, 258, 267, 279, 432, 540
MboI	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
MboII	GAAGA	7 cut(s)	70, 79, 479, 482, 1274, 1288, 1378
MfeI	CAATTG	1 cut(s)	1268
MflI	RGATCY	1 cut(s)	256
MhlI	GDGCHC	1 cut(s)	853
MluCI	AATT	9 cut(s)	31, 185, 311, 331, 458, 470, 715, 924, 1268
MlyI	GAGTC	1 cut(s)	874
MmeI	TCCRAC	1 cut(s)	60
MnlI	CCTC	5 cut(s)	247, 263, 708, 953, 1239
MseI	TTAA	3 cut(s)	510, 885, 1005
MslI	CAYNNNNRTG	1 cut(s)	594
MspA1I	CMGCKG	2 cut(s)	320, 653
MspI	CCGG	1 cut(s)	1317
MspR9I	CCNGG	2 cut(s)	609, 1049
MunI	CAATTG	1 cut(s)	1268
Mva1269I	GAATGC	1 cut(s)	1326
MvaI	CCWGG	2 cut(s)	609, 1049
MvnI	CGCG	1 cut(s)	637
MwoI	GCNNNNNNNGC	4 cut(s)	157, 656, 659, 1123
NdeII	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
NlaIII	CATG	5 cut(s)	127, 599, 686, 815, 1190
NlaIV	GGNNCC	1 cut(s)	258
NmuCI	GTSAC	1 cut(s)	1147
PctI	GAATGC	1 cut(s)	1326
PfeI	GAWTC	1 cut(s)	16
PkrI	GCNGC	6 cut(s)	319, 322, 652, 661, 1116, 1128
PleI	GAGTC	1 cut(s)	874
PpsI	GAGTC	1 cut(s)	874
PsiI	TTATAA	1 cut(s)	929
Psp6I	CCWGG	2 cut(s)	607, 1047
PspGI	CCWGG	2 cut(s)	607, 1047
PspN4I	GGNNCC	1 cut(s)	258
PspPI	GGNCC	1 cut(s)	791
PsuI	RGATCY	1 cut(s)	256
PvuII	CAGCTG	1 cut(s)	320
RsaI	GTAC	2 cut(s)	1076, 1228
RsaNI	GTAC	2 cut(s)	1075, 1227
RseI	CAYNNNNRTG	1 cut(s)	594
SaqAI	TTAA	3 cut(s)	510, 885, 1005
SatI	GCNGC	6 cut(s)	318, 321, 651, 660, 1115, 1127
Sau3AI	GATC	7 cut(s)	100, 105, 256, 265, 277, 430, 538
Sau96I	GGNCC	1 cut(s)	791
SchI	GAGTC	1 cut(s)	874
ScrFI	CCNGG	2 cut(s)	609, 1049
SduI	GDGCHC	1 cut(s)	853
SexAI	ACCWGGT	1 cut(s)	607
SfaNI	GCATC	1 cut(s)	160
SfcI	CTRYAG	1 cut(s)	1397
SmiMI	CAYNNNNRTG	1 cut(s)	594
SpeI	ACTAGT	1 cut(s)	230
Sse9I	AATT	9 cut(s)	31, 185, 311, 331, 458, 470, 715, 924, 1268
SsiI	CCGC	4 cut(s)	530, 651, 1094, 1126
SspI	AATATT	1 cut(s)	733
SspMI	CTAG	4 cut(s)	231, 327, 572, 1236
StyD4I	CCNGG	2 cut(s)	607, 1047
StyI	CCWWGG	1 cut(s)	571
TaaI	ACNGT	1 cut(s)	41
TaiI	ACGT	2 cut(s)	391, 839
TaqI	TCGA	2 cut(s)	268, 373
TaqII	GACCGA	1 cut(s)	1026
TasI	AATT	9 cut(s)	31, 185, 311, 331, 458, 470, 715, 924, 1268
TatI	WGTACW	1 cut(s)	1226
TauI	GCSGC	2 cut(s)	653, 1129
TfiI	GAWTC	1 cut(s)	16
Tru1I	TTAA	3 cut(s)	510, 885, 1005
Tru9I	TTAA	3 cut(s)	510, 885, 1005
TscAI	CASTG	1 cut(s)	1359
TseFI	GTSAC	1 cut(s)	1147
TseI	GCWGC	4 cut(s)	317, 320, 659, 1114
Tsp45I	GTSAC	1 cut(s)	1147
TspDTI	ATGAA	3 cut(s)	573, 656, 1203
TspRI	CASTG	1 cut(s)	1359
XapI	RAATTY	3 cut(s)	331, 458, 715
XmaJI	CCTAGG	1 cut(s)	571
XspI	CTAG	4 cut(s)	231, 327, 572, 1236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.