Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003976

Physical Location & Seq

Genomic Coordinates Forward (+)

20424344 .. 20427377

Gene Structure 3034 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1129100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1182 bp

ATGGGTTGGATGATGATTCCCTGTTCTGGTAATTCCAACAGTAAAGCAAAAACCAAGAAGAAAAGGAAGAACAAGATTGAGTTGCAGGAAAAGCCACTTGATCAGATCAAACCCACTTCAGGTTTTTCAAAGACTAATTCCATCTTGAGTGTCAAGGAGGATCCGAAAGATGGGGGGTCTGATCACATTGCGGCACAGACTTTTGCATTCCGTGAATTGGCAGCCGCAACTAGAAATTTCAGGTCAGAATGTCTATTGGGTGAGGGTGGATTTGGTCGAGTATACAAAGGACGTTTGGAAAGTACCAATCAGGTAGTAGCTATCAAACAACTTGATCGTGATGGATTACAAGGGAACAGGGAATTCCTTGTTGAAGTGTTGATGTTGAGTCTTCTTCACCATCCCAATCTTGTTAATCTTACTGGTTATTGTGCGGATGGAGATCAAAGGCTTCTCGTTTATGAATATATGCCCCTAGGGTCTTTGGACGACCATTTACATGATGTGTCACCTGGTAAGAGACGACTTGATTGGAATACACGAATGAAAATAGCCGCTGGAGCAGCAAAGGGATTGGAGTATCTACATGACAAAGCAAGTCCTCCTGTTATATACAGAGATTTGAAATGCTCCAACATATTACTCGGTGAAGGATATCATCCAAAGCTGTCTGATTTTGGTTTAGCCAAACTTGGGCCTGTTGGGGATAACACCCATGTATCTACGAGGGTTATGGGAACGTATGGATATTGTGCTCCAGAGTATGCAATGACAGGGCAGTTGACTCTTAAATCAGACGTTTATAGCTTTGGCGTAGTTCTTTTGGAAATTATAACGGGCAGGAGAGCGATTGACAATACCAGAGGTACAGGAGAACAGAATTTGGTTGCATGGGCAAGACCCTTGTTTAAAGACCGAAAGAAATTTTCACAAATGGCAGACCCAACGCTCCAGGGTCAGTATCCCCCAAGGGGCTTGTACCAAGCTCTTGCAGTTGCGGCAATGTGTGTTCAGGAGCAGCCTAATATGCGGCCAGTTATAGCTGATGTCGTCACAGCTTTGACTTACCTTGCTTCACAGAAGTATGACCCTGAAACGCAGCCAGTCCAAAGCTCACGTCTTGCCCCTTGTACTCCCCCTAGAACCAAGAGGGATAGTGAAAGGAAAGCGCAATTGTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009966 GO:0009967 GO:0009987 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023051 GO:0023052 GO:0023056 GO:0031347 GO:0031349 GO:0036211 GO:0038023 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1900457 GO:1900459 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

394

Amino Acids

43.68

Weight (kDa)

9.25

Isoelectric Point (pI)

37.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	83 - 351	5.1e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	84 - 353	3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G02800
fragaria_vesca	FvH4_6g15040 FvH4_7g20570
malus_domestica	MD01G1114700.v1.1 MD04G1112600.v1.1 MD04G1113000.v1.1 MD07G1182700.v1.1 MD12G1129100.v1.1
prunus_persica	Prupe.2G221400_v2.0.a1 Prupe.2G221400_v2.0.a1 Prupe.6G244400_v2.0.a1 Prupe.6G244400_v2.0.a1
pyrus_communis	pycom01g14360 pycom04g10250 pycom04g10290 pycom12g12540
rosa_chinensis	RchiOBHm_Chr1g0364451 RchiOBHm_Chr3g0468211
rosa_laevigata	RLG00000024441 RLG00000027478
rosa_multiflora	Rmu_sc0001342.1_g000009 Rmu_sc0004524.1_g000006
rosa_roxburghii	Rroxscaffold_4G00291820 Rroxscaffold_4G00292260 Rroxscaffold_6G00412910
rosa_rugosa	Rorug01G0316100 Rorug03G0094200
rosa_samantha	Rh1AG324600 Rh1BG287300 Rh1DG318800 Rh3DG164000
rosa_wichuraiana	Rw1G028770 Rw3G013430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	833
AccI	GTMKAC	1 cut(s)	282
AciI	CCGC	6 cut(s)	191, 225, 434, 555, 998, 1030
AclWI	GGATC	2 cut(s)	155, 168
AcoI	YGGCCR	1 cut(s)	1031
AcsI	RAATTY	4 cut(s)	235, 362, 880, 923
AcuI	CTGAAG	1 cut(s)	102
AfaI	GTAC	4 cut(s)	304, 868, 980, 1132
AfiI	CCNNNNNNNGG	6 cut(s)	26, 119, 170, 217, 693, 971
AgsI	TTSAA	3 cut(s)	129, 374, 625
AjiI	CACGTC	1 cut(s)	1118
AjnI	CCWGG	2 cut(s)	511, 951
AluBI	AGCT	7 cut(s)	320, 667, 807, 986, 1043, 1058, 1113
AluI	AGCT	7 cut(s)	320, 667, 807, 986, 1043, 1058, 1113
Alw21I	GWGCWC	1 cut(s)	757
Alw26I	GTCTC	1 cut(s)	514
AlwI	GGATC	2 cut(s)	155, 168
AoxI	GGCC	2 cut(s)	695, 1031
ApeKI	GCWGC	4 cut(s)	221, 563, 1018, 1099
ApoI	RAATTY	4 cut(s)	235, 362, 880, 923
ArsI	GACNNNNNNTTYG	2 cut(s)	1102, 1134
AspA2I	CCTAGG	1 cut(s)	475
AspLEI	GCGC	1 cut(s)	1171
AspS9I	GGNCC	1 cut(s)	695
AsuHPI	GGTGA	4 cut(s)	272, 389, 501, 659
AvrII	CCTAGG	1 cut(s)	475
BaeI	ACNNNNGTAYC	2 cut(s)	702, 735
BamHI	GGATCC	1 cut(s)	160
BbsI	GAAGAC	1 cut(s)	383
Bbv12I	GWGCWC	1 cut(s)	757
BbvI	GCAGC	4 cut(s)	233, 575, 1030, 1111
BccI	CCATC	5 cut(s)	149, 164, 335, 408, 431
BciT130I	CCWGG	2 cut(s)	513, 953
BciVI	GTATCC	1 cut(s)	972
BclI	TGATCA	2 cut(s)	100, 181
BcoDI	GTCTC	1 cut(s)	514
BfaI	CTAG	3 cut(s)	231, 476, 1140
BfuI	GTATCC	1 cut(s)	972
BisI	GCNGC	9 cut(s)	192, 222, 225, 555, 564, 999, 1019, 1031, 1100
BlnI	CCTAGG	1 cut(s)	475
BlsI	GCNGC	9 cut(s)	193, 223, 226, 556, 565, 1000, 1020, 1032, 1101
Bme1390I	CCNGG	2 cut(s)	513, 953
BmgBI	CACGTC	1 cut(s)	1118
BmgT120I	GGNCC	1 cut(s)	695
BmiI	GGNNCC	1 cut(s)	162
BmrFI	CCNGG	2 cut(s)	513, 953
BpiI	GAAGAC	1 cut(s)	383
BpmI	CTGGAG	3 cut(s)	579, 741, 935
BpuEI	CTTGAG	1 cut(s)	166
BsaBI	GATNNNNATC	1 cut(s)	441
BsaJI	CCNNGG	3 cut(s)	475, 952, 968
Bsc4I	CCNNNNNNNGG	6 cut(s)	26, 119, 170, 217, 693, 971
Bse1I	ACTGG	3 cut(s)	427, 1034, 1103
Bse3DI	GCAATG	3 cut(s)	186, 774, 1008
Bse8I	GATNNNNATC	1 cut(s)	441
BseBI	CCWGG	2 cut(s)	513, 953
BseDI	CCNNGG	3 cut(s)	475, 952, 968
BseGI	GGATG	4 cut(s)	15, 400, 442, 658
BseJI	GATNNNNATC	1 cut(s)	441
BseLI	CCNNNNNNNGG	6 cut(s)	26, 119, 170, 217, 693, 971
BseMI	GCAATG	3 cut(s)	186, 774, 1008
BseNI	ACTGG	3 cut(s)	427, 1034, 1103
BseXI	GCAGC	4 cut(s)	233, 575, 1030, 1111
BshFI	GGCC	2 cut(s)	697, 1033
BsiHKAI	GWGCWC	1 cut(s)	757
BslI	CCNNNNNNNGG	6 cut(s)	26, 119, 170, 217, 693, 971
BsmAI	GTCTC	1 cut(s)	514
BsmBI	CGTCTC	1 cut(s)	514
BsmI	GAATGC	1 cut(s)	206
BsnI	GGCC	2 cut(s)	697, 1033
Bsp1286I	GDGCHC	1 cut(s)	757
Bsp143I	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
BspACI	CCGC	6 cut(s)	191, 225, 434, 555, 998, 1030
BspANI	GGCC	2 cut(s)	697, 1033
BspLI	GGNNCC	1 cut(s)	162
BspPI	GGATC	2 cut(s)	155, 168
BsrDI	GCAATG	3 cut(s)	186, 774, 1008
BsrI	ACTGG	3 cut(s)	427, 1034, 1103
BssECI	CCNNGG	3 cut(s)	475, 952, 968
BssMI	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
BssNAI	GTATAC	1 cut(s)	283
BssT1I	CCWWGG	2 cut(s)	475, 968
Bst1107I	GTATAC	1 cut(s)	283
Bst2UI	CCWGG	2 cut(s)	513, 953
Bst4CI	ACNGT	1 cut(s)	41
BstF5I	GGATG	4 cut(s)	15, 400, 442, 658
BstHHI	GCGC	1 cut(s)	1171
BstKTI	GATC	6 cut(s)	103, 108, 163, 184, 337, 445
BstMAI	GTCTC	1 cut(s)	514
BstMBI	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
BstMWI	GCNNNNNNNGC	5 cut(s)	91, 560, 563, 998, 1027
BstNI	CCWGG	2 cut(s)	513, 953
BstSCI	CCNGG	2 cut(s)	511, 951
BstV1I	GCAGC	4 cut(s)	233, 575, 1030, 1111
BstV2I	GAAGAC	1 cut(s)	383
BstX2I	RGATCY	1 cut(s)	160
BstYI	RGATCY	1 cut(s)	160
BstZ17I	GTATAC	1 cut(s)	283
BsuI	GTATCC	1 cut(s)	972
BsuRI	GGCC	2 cut(s)	697, 1033
BtrI	CACGTC	1 cut(s)	1118
BtsCI	GGATG	4 cut(s)	15, 400, 442, 658
CfoI	GCGC	1 cut(s)	1171
Cfr13I	GGNCC	1 cut(s)	695
CsiI	ACCWGGT	1 cut(s)	511
Csp6I	GTAC	4 cut(s)	303, 867, 979, 1131
CviAII	CATG	4 cut(s)	500, 587, 716, 891
CviQI	GTAC	4 cut(s)	303, 867, 979, 1131
DpnI	GATC	6 cut(s)	102, 107, 162, 183, 336, 444
DpnII	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
DraI	TTTAAA	1 cut(s)	910
EaeI	YGGCCR	1 cut(s)	1031
Eco130I	CCWWGG	2 cut(s)	475, 968
Eco32I	GATATC	1 cut(s)	656
Eco57I	CTGAAG	1 cut(s)	102
EcoRI	GAATTC	1 cut(s)	362
EcoRII	CCWGG	2 cut(s)	511, 951
EcoRV	GATATC	1 cut(s)	656
EcoT14I	CCWWGG	2 cut(s)	475, 968
ErhI	CCWWGG	2 cut(s)	475, 968
Esp3I	CGTCTC	1 cut(s)	514
FaeI	CATG	4 cut(s)	503, 590, 719, 894
FatI	CATG	4 cut(s)	499, 586, 715, 890
FbaI	TGATCA	2 cut(s)	100, 181
FblI	GTMKAC	1 cut(s)	282
Fnu4HI	GCNGC	9 cut(s)	192, 222, 225, 555, 564, 999, 1019, 1031, 1100
FokI	GGATG	4 cut(s)	22, 387, 449, 645
Fsp4HI	GCNGC	9 cut(s)	192, 222, 225, 555, 564, 999, 1019, 1031, 1100
FspBI	CTAG	3 cut(s)	231, 476, 1140
GlaI	GCGC	1 cut(s)	1170
GluI	GCNGC	9 cut(s)	192, 222, 225, 555, 564, 999, 1019, 1031, 1100
GsuI	CTGGAG	3 cut(s)	579, 741, 935
HaeIII	GGCC	2 cut(s)	697, 1033
HhaI	GCGC	1 cut(s)	1171
Hin1II	CATG	4 cut(s)	503, 590, 719, 894
Hin6I	GCGC	1 cut(s)	1169
HinP1I	GCGC	1 cut(s)	1169
HincII	GTYRAC	1 cut(s)	783
HindII	GTYRAC	1 cut(s)	783
HinfI	GANTC	3 cut(s)	16, 388, 784
HphI	GGTGA	4 cut(s)	272, 389, 501, 659
Hpy166II	GTNNAC	2 cut(s)	283, 783
Hpy188I	TCNGA	6 cut(s)	105, 165, 181, 247, 673, 796
Hpy188III	TCNNGA	4 cut(s)	145, 338, 758, 1013
Hpy8I	GTNNAC	2 cut(s)	283, 783
HpyAV	CCTTC	1 cut(s)	644
HpyCH4III	ACNGT	1 cut(s)	41
HpyCH4IV	ACGT	4 cut(s)	292, 740, 798, 1117
HpyCH4V	TGCA	5 cut(s)	85, 206, 767, 890, 992
HpyF10VI	GCNNNNNNNGC	5 cut(s)	91, 560, 563, 998, 1027
HpySE526I	ACGT	4 cut(s)	292, 740, 798, 1117
Hsp92II	CATG	4 cut(s)	503, 590, 719, 894
HspAI	GCGC	1 cut(s)	1169
Ksp22I	TGATCA	2 cut(s)	100, 181
Kzo9I	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
LmnI	GCTCC	5 cut(s)	560, 635, 760, 954, 1015
Lsp1109I	GCAGC	4 cut(s)	233, 575, 1030, 1111
MabI	ACCWGGT	1 cut(s)	511
MaeI	CTAG	3 cut(s)	231, 476, 1140
MaeII	ACGT	4 cut(s)	292, 740, 798, 1117
MaeIII	GTNAC	2 cut(s)	507, 1051
MalI	GATC	6 cut(s)	102, 107, 162, 183, 336, 444
MboI	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
MboII	GAAGA	4 cut(s)	70, 79, 383, 386
MfeI	CAATTG	1 cut(s)	1172
MflI	RGATCY	1 cut(s)	160
MhlI	GDGCHC	1 cut(s)	757
MluCI	AATT	9 cut(s)	31, 136, 215, 235, 362, 828, 880, 923, 1172
MlyI	GAGTC	2 cut(s)	397, 778
MmeI	TCCRAC	2 cut(s)	60, 657
MnlI	CCTC	6 cut(s)	151, 256, 612, 720, 857, 1143
MseI	TTAA	3 cut(s)	414, 789, 909
MslI	CAYNNNNRTG	1 cut(s)	498
MspA1I	CMGCKG	1 cut(s)	557
MspR9I	CCNGG	2 cut(s)	513, 953
MunI	CAATTG	1 cut(s)	1172
Mva1269I	GAATGC	1 cut(s)	206
MvaI	CCWGG	2 cut(s)	513, 953
MwoI	GCNNNNNNNGC	5 cut(s)	91, 560, 563, 998, 1027
NdeII	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
NlaIII	CATG	4 cut(s)	503, 590, 719, 894
NlaIV	GGNNCC	1 cut(s)	162
NmuCI	GTSAC	2 cut(s)	507, 1051
PctI	GAATGC	1 cut(s)	206
PfeI	GAWTC	1 cut(s)	16
PkrI	GCNGC	9 cut(s)	193, 223, 226, 556, 565, 1000, 1020, 1032, 1101
PleI	GAGTC	2 cut(s)	396, 778
PpsI	GAGTC	2 cut(s)	396, 778
PsiI	TTATAA	1 cut(s)	833
Psp6I	CCWGG	2 cut(s)	511, 951
PspGI	CCWGG	2 cut(s)	511, 951
PspN4I	GGNNCC	1 cut(s)	162
PspPI	GGNCC	1 cut(s)	695
PsuI	RGATCY	1 cut(s)	160
RsaI	GTAC	4 cut(s)	304, 868, 980, 1132
RsaNI	GTAC	4 cut(s)	303, 867, 979, 1131
RseI	CAYNNNNRTG	1 cut(s)	498
SaqAI	TTAA	3 cut(s)	414, 789, 909
SatI	GCNGC	9 cut(s)	192, 222, 225, 555, 564, 999, 1019, 1031, 1100
Sau3AI	GATC	6 cut(s)	100, 105, 160, 181, 334, 442
Sau96I	GGNCC	1 cut(s)	695
SchI	GAGTC	2 cut(s)	397, 778
ScrFI	CCNGG	2 cut(s)	513, 953
SduI	GDGCHC	1 cut(s)	757
SexAI	ACCWGGT	1 cut(s)	511
SmiMI	CAYNNNNRTG	1 cut(s)	498
SmlI	CTYRAG	1 cut(s)	145
SmoI	CTYRAG	1 cut(s)	145
Sse9I	AATT	9 cut(s)	31, 136, 215, 235, 362, 828, 880, 923, 1172
SsiI	CCGC	6 cut(s)	191, 225, 434, 555, 998, 1030
SspMI	CTAG	3 cut(s)	231, 476, 1140
StyD4I	CCNGG	2 cut(s)	511, 951
StyI	CCWWGG	2 cut(s)	475, 968
TaaI	ACNGT	1 cut(s)	41
TaiI	ACGT	4 cut(s)	295, 743, 801, 1120
TaqI	TCGA	1 cut(s)	277
TaqII	GACCGA	1 cut(s)	930
TasI	AATT	9 cut(s)	31, 136, 215, 235, 362, 828, 880, 923, 1172
TatI	WGTACW	1 cut(s)	1130
TauI	GCSGC	5 cut(s)	194, 227, 557, 1001, 1033
TfiI	GAWTC	1 cut(s)	16
Tru1I	TTAA	3 cut(s)	414, 789, 909
Tru9I	TTAA	3 cut(s)	414, 789, 909
TseFI	GTSAC	2 cut(s)	507, 1051
TseI	GCWGC	4 cut(s)	221, 563, 1018, 1099
Tsp45I	GTSAC	2 cut(s)	507, 1051
TspDTI	ATGAA	2 cut(s)	477, 560
TspGWI	ACGGA	1 cut(s)	200
XapI	RAATTY	4 cut(s)	235, 362, 880, 923
XmaJI	CCTAGG	1 cut(s)	475
XmiI	GTMKAC	1 cut(s)	282
XspI	CTAG	3 cut(s)	231, 476, 1140

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.