MD04G1153000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004339
Physical Location & Seq
Forward (+)
24156180 .. 24156761
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1153000.v1.1.491

Sequence Viewer

Length: 531 bp
ATGAGCAGACACCCAAAAACTGGCGACTGGTACCCTGCAATTAACAAACCAGCTGGAGTTGTGCACTGGCTTAAGCACAGTAAGGAGGCTGAGAATGTTGATTGGGAACTTGGTGCTGAGAAAGGCAAGCCTATTGCAGCATACTATGGGTACTTGGTTGGACGCGATAATCTTCTCGCTCGACTGCACACAAAGCACCCTAAACTCTGTGATAAAGTTGGTGGGCTTTTGGCCATGCATATAGATGATCTTCGAGCATTGGCACCCGTGTGGCTTTCAAAAACAGAAGAAGTGCAGGAAGATAGAGCTCACTGGACAACTAATATAACTGGTGACATCTATGGGAAAGGATGGATCAGTGAGATGTATGGATATTCTTTTGGTGCAGCAGAGGTAGGTCTTCAGCACAAAATAAATAATAACTTGATGATCTACCCAGGCTATACTCCACAAGAGGGTGTTGTTGGGTTAAGAAAGGTTGTCTTTGTTTGCGGTGGCCATCGTAGACAAGAACTAAGCAGATTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.84

Weight (kDa)

8.46

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HPAT PF23452 39 - 151 4.2e-10 Hydroxyproline O-arabinosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 30
AccB1I GGYRCC 2 cut(s) 30, 262
AccB7I CCANNNNNTGG 1 cut(s) 20
AccI GTMKAC 1 cut(s) 505
AccII CGCG 1 cut(s) 165
AciI CCGC 1 cut(s) 492
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 2 cut(s) 231, 496
AcuI CTGAAG 1 cut(s) 386
AfaI GTAC 2 cut(s) 32, 152
AfiI CCNNNNNNNGG 2 cut(s) 20, 455
AflII CTTAAG 1 cut(s) 71
AgsI TTSAA 1 cut(s) 279
AjnI CCWGG 1 cut(s) 436
AleI CACNNNNGTG 1 cut(s) 268
AluBI AGCT 2 cut(s) 53, 308
AluI AGCT 2 cut(s) 53, 308
Alw21I GWGCWC 2 cut(s) 66, 310
Alw44I GTGCAC 1 cut(s) 62
AlwI GGATC 1 cut(s) 362
AoxI GGCC 2 cut(s) 231, 496
ApaLI GTGCAC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 137, 386
Asp718I GGTACC 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 2 cut(s) 233, 498
BanI GGYRCC 2 cut(s) 30, 262
BanII GRGCYC 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 2 cut(s) 66, 310
BbvI GCAGC 2 cut(s) 149, 398
BccI CCATC 2 cut(s) 345, 507
BciT130I CCWGG 1 cut(s) 438
BfrI CTTAAG 1 cut(s) 71
BisI GCNGC 2 cut(s) 138, 387
BlsI GCNGC 2 cut(s) 139, 388
Bme1390I CCNGG 1 cut(s) 438
BmiI GGNNCC 2 cut(s) 32, 264
BmrFI CCNGG 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 392
BpmI CTGGAG 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 436
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 455
Bse1I ACTGG 5 cut(s) 25, 32, 71, 317, 334
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 1 cut(s) 356
BseLI CCNNNNNNNGG 2 cut(s) 20, 455
BseMII CTCAG 2 cut(s) 81, 108
BseNI ACTGG 5 cut(s) 25, 32, 71, 317, 334
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 2 cut(s) 149, 398
BsgI GTGCAG 3 cut(s) 170, 314, 405
Bsh1236I CGCG 1 cut(s) 165
BshFI GGCC 2 cut(s) 233, 498
BshNI GGYRCC 2 cut(s) 30, 262
BsiHKAI GWGCWC 2 cut(s) 66, 310
BslI CCNNNNNNNGG 2 cut(s) 20, 455
BsnI GGCC 2 cut(s) 233, 498
Bsp1286I GDGCHC 2 cut(s) 66, 310
Bsp143I GATC 3 cut(s) 247, 354, 429
BspACI CCGC 1 cut(s) 492
BspANI GGCC 2 cut(s) 233, 498
BspCNI CTCAG 2 cut(s) 82, 109
BspFNI CGCG 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 32, 264
BspPI GGATC 1 cut(s) 362
BspT107I GGYRCC 2 cut(s) 30, 262
BspTI CTTAAG 1 cut(s) 71
BsrI ACTGG 5 cut(s) 25, 32, 71, 317, 334
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 3 cut(s) 247, 354, 429
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 1 cut(s) 80
BstAFI CTTAAG 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 128
BstDEI CTNAG 3 cut(s) 90, 117, 515
BstF5I GGATG 1 cut(s) 356
BstFNI CGCG 1 cut(s) 165
BstKTI GATC 3 cut(s) 250, 357, 432
BstMBI GATC 3 cut(s) 247, 354, 429
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNI CCWGG 1 cut(s) 438
BstSCI CCNGG 1 cut(s) 436
BstSLI GKGCMC 1 cut(s) 66
BstUI CGCG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 149, 398
BstV2I GAAGAC 1 cut(s) 392
BsuRI GGCC 2 cut(s) 233, 498
BtsCI GGATG 1 cut(s) 356
BtsIMutI CAGTG 3 cut(s) 64, 310, 364
Cac8I GCNNGC 1 cut(s) 128
CseI GACGC 1 cut(s) 171
Csp6I GTAC 2 cut(s) 31, 151
CviAII CATG 1 cut(s) 235
CviQI GTAC 2 cut(s) 31, 151
DdeI CTNAG 3 cut(s) 90, 117, 515
DpnI GATC 3 cut(s) 249, 356, 431
DpnII GATC 3 cut(s) 247, 354, 429
EaeI YGGCCR 2 cut(s) 231, 496
Ecl136II GAGCTC 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 310
FaeI CATG 1 cut(s) 238
FaiI YATR 9 cut(s) 142, 147, 236, 240, 242, 326, 342, 369, 444
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 1 cut(s) 234
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 2 cut(s) 138, 387
FokI GGATG 1 cut(s) 363
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 2 cut(s) 138, 387
GluI GCNGC 2 cut(s) 138, 387
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 2 cut(s) 233, 498
HgaI GACGC 1 cut(s) 171
Hin1II CATG 1 cut(s) 238
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 2 cut(s) 64, 506
Hpy8I GTNNAC 2 cut(s) 64, 506
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 7 cut(s) 38, 64, 137, 187, 238, 295, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpyF3I CTNAG 3 cut(s) 90, 117, 515
Hsp92II CATG 1 cut(s) 238
KpnI GGTACC 1 cut(s) 34
Kzo9I GATC 3 cut(s) 247, 354, 429
Lsp1109I GCAGC 2 cut(s) 149, 398
MaeIII GTNAC 1 cut(s) 332
MalI GATC 3 cut(s) 249, 356, 431
MboI GATC 3 cut(s) 247, 354, 429
MboII GAAGA 5 cut(s) 164, 242, 299, 311, 392
MhlI GDGCHC 2 cut(s) 66, 310
MlsI TGGCCA 2 cut(s) 233, 498
MluCI AATT 1 cut(s) 39
MluNI TGGCCA 2 cut(s) 233, 498
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 3 cut(s) 79, 385, 448
Mox20I TGGCCA 2 cut(s) 233, 498
Mph1103I ATGCAT 1 cut(s) 240
MscI TGGCCA 2 cut(s) 233, 498
MseI TTAA 3 cut(s) 42, 72, 470
MslI CAYNNNNRTG 2 cut(s) 243, 268
Msp20I TGGCCA 2 cut(s) 233, 498
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 438
MvnI CGCG 1 cut(s) 165
MwoI GCNNNNNNNGC 1 cut(s) 193
NdeII GATC 3 cut(s) 247, 354, 429
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 2 cut(s) 32, 264
NmuCI GTSAC 1 cut(s) 332
NsiI ATGCAT 1 cut(s) 240
OliI CACNNNNGTG 1 cut(s) 268
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 2 cut(s) 139, 388
Psp124BI GAGCTC 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 32, 264
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 2 cut(s) 32, 152
RsaNI GTAC 2 cut(s) 31, 151
RseI CAYNNNNRTG 2 cut(s) 243, 268
SacI GAGCTC 1 cut(s) 310
SaqAI TTAA 3 cut(s) 42, 72, 470
SatI GCNGC 2 cut(s) 138, 387
Sau3AI GATC 3 cut(s) 247, 354, 429
ScrFI CCNGG 1 cut(s) 438
SduI GDGCHC 2 cut(s) 66, 310
SetI ASST 5 cut(s) 55, 310, 396, 400, 480
SmiMI CAYNNNNRTG 2 cut(s) 243, 268
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 1 cut(s) 39
SsiI CCGC 1 cut(s) 492
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 1 cut(s) 80
TaqI TCGA 2 cut(s) 181, 253
TasI AATT 1 cut(s) 39
Tru1I TTAA 3 cut(s) 42, 72, 470
Tru9I TTAA 3 cut(s) 42, 72, 470
TscAI CASTG 3 cut(s) 71, 317, 364
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 137, 386
Tsp45I GTSAC 1 cut(s) 332
TspRI CASTG 3 cut(s) 71, 317, 364
Van91I CCANNNNNTGG 1 cut(s) 20
Vha464I CTTAAG 1 cut(s) 71
VneI GTGCAC 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 505
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.