MD16G1283700.v1.1

Bifunctional nuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004339
Physical Location & Seq
Forward (+)
40157454 .. 40160410
2957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1283700.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGGGTTAGCCTGGATGGATCTTTTGGGTTATGTATGTTTAATCATATGTTATATTGTTTCTGGTGCTACAGAGGGCCCAAATTCTTTAATTCAAGAACTTGGTTCGGTTAAGAATATAAATTTAGCTATCAAAGAGGAAAGATATAAAGATGCAGCCCTCCTACTATGTTTCTCCACCATTGCCCCAATTTTGTCGAAAACAACAGCAGCAACCTACGGCTCTCTTAGCTCAGGAGTGAGGCGACCGATGGTCAATGAGCTCCCATCGTTTCCTGGTTGCCTTCGTTGTGGTTGGAAAAGCTATAGACTGCAATGTAATAAGGTCGTGATTCTGGATGCTGACATGATTATATGTGGCCCGATTGTTCCTTGGAAACTTAGTGCTGAGAAAGCAAGCCTATTGCAGCGTATATGGGTCAAGCTCTCTAAGTACATTGGGGATAAGCATGTGATTCTCTTTTTTTACGCATTGGACTTCACATTCGTTTGTCATAGATCACCTCTCTCAACGACCGCCACACTGAGTTTGCGGAGCTCAACACAAAAATCTTGGGTGTTTCAGTTGAAAGTGTGGCTTGTTGCCTTGCTCCTCAATATATTCTATAACACAAAAATGTGTTGGAATTTTGCGTCAATAGTTTTTTGTTTTGAAATCCCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNase-RNase UVR

Protein Analysis

222

Amino Acids

24.85

Weight (kDa)

9.1

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 516, 532
AclWI GGATC 1 cut(s) 27
AcsI RAATTY 3 cut(s) 82, 121, 625
AfaI GTAC 1 cut(s) 434
AgsI TTSAA 3 cut(s) 95, 568, 653
AhdI GACNNNNNGTC 1 cut(s) 251
AjnI CCWGG 2 cut(s) 11, 274
AluBI AGCT 6 cut(s) 128, 231, 262, 303, 424, 537
AluI AGCT 6 cut(s) 128, 231, 262, 303, 424, 537
Alw21I GWGCWC 2 cut(s) 264, 539
AlwI GGATC 1 cut(s) 27
AoxI GGCC 2 cut(s) 76, 358
ApaI GGGCCC 1 cut(s) 80
ApeKI GCWGC 3 cut(s) 155, 209, 406
ApoI RAATTY 3 cut(s) 82, 121, 625
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AspS9I GGNCC 3 cut(s) 76, 77, 359
AsuHPI GGTGA 1 cut(s) 492
BaeGI GKGCMC 1 cut(s) 80
BanII GRGCYC 3 cut(s) 80, 264, 539
Bbv12I GWGCWC 2 cut(s) 264, 539
BbvI GCAGC 3 cut(s) 167, 221, 418
BccI CCATC 3 cut(s) 10, 244, 274
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 2 cut(s) 13, 276
BfmI CTRYAG 2 cut(s) 69, 304
BisI GCNGC 3 cut(s) 156, 210, 407
BlsI GCNGC 3 cut(s) 157, 211, 408
Bme1390I CCNGG 2 cut(s) 13, 276
BmeRI GACNNNNNGTC 1 cut(s) 251
BmgT120I GGNCC 3 cut(s) 76, 77, 359
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 2 cut(s) 13, 276
BmsI GCATC 2 cut(s) 142, 328
Bpu10I CCTNAGC 1 cut(s) 232
BsaJI CCNNGG 1 cut(s) 371
Bse3DI GCAATG 2 cut(s) 180, 320
BseBI CCWGG 2 cut(s) 13, 276
BseDI CCNNGG 1 cut(s) 371
BseGI GGATG 2 cut(s) 21, 343
BseMI GCAATG 2 cut(s) 180, 320
BseMII CTCAG 3 cut(s) 246, 378, 515
BseRI GAGGAG 1 cut(s) 581
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 3 cut(s) 167, 221, 418
Bsh1285I CGRYCG 2 cut(s) 248, 516
BshFI GGCC 2 cut(s) 78, 360
BsiEI CGRYCG 2 cut(s) 248, 516
BsiHKAI GWGCWC 2 cut(s) 264, 539
BsnI GGCC 2 cut(s) 78, 360
Bsp120I GGGCCC 1 cut(s) 76
Bsp1286I GDGCHC 3 cut(s) 80, 264, 539
Bsp143I GATC 2 cut(s) 19, 497
BspACI CCGC 2 cut(s) 516, 532
BspANI GGCC 2 cut(s) 78, 360
BspCNI CTCAG 3 cut(s) 245, 379, 516
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 27
BsrDI GCAATG 2 cut(s) 180, 320
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 2 cut(s) 19, 497
BssT1I CCWWGG 1 cut(s) 371
Bst2UI CCWGG 2 cut(s) 13, 276
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 6 cut(s) 227, 232, 380, 387, 429, 524
BstF5I GGATG 2 cut(s) 21, 343
BstKTI GATC 2 cut(s) 22, 500
BstMBI GATC 2 cut(s) 19, 497
BstMCI CGRYCG 2 cut(s) 248, 516
BstMWI GCNNNNNNNGC 2 cut(s) 228, 392
BstNI CCWGG 2 cut(s) 13, 276
BstNSI RCATGY 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 11, 274
BstSFI CTRYAG 2 cut(s) 69, 304
BstSLI GKGCMC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 167, 221, 418
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuRI GGCC 2 cut(s) 78, 360
BtsCI GGATG 2 cut(s) 21, 343
BtsIMutI CAGTG 1 cut(s) 521
Cac8I GCNNGC 1 cut(s) 397
Cfr13I GGNCC 3 cut(s) 76, 77, 359
CseI GACGC 1 cut(s) 621
Csp6I GTAC 1 cut(s) 433
CviAII CATG 2 cut(s) 346, 449
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 6 cut(s) 227, 232, 380, 387, 429, 524
DpnI GATC 2 cut(s) 21, 499
DpnII GATC 2 cut(s) 19, 497
DriI GACNNNNNGTC 1 cut(s) 251
Eam1105I GACNNNNNGTC 1 cut(s) 251
Ecl136II GAGCTC 2 cut(s) 262, 537
Eco130I CCWWGG 1 cut(s) 371
Eco24I GRGCYC 3 cut(s) 80, 264, 539
Eco53kI GAGCTC 2 cut(s) 262, 537
EcoICRI GAGCTC 2 cut(s) 262, 537
EcoO109I RGGNCCY 1 cut(s) 76
EcoRII CCWGG 2 cut(s) 11, 274
EcoT14I CCWWGG 1 cut(s) 371
EcoT38I GRGCYC 3 cut(s) 80, 264, 539
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 2 cut(s) 349, 452
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FatI CATG 2 cut(s) 345, 448
FauNDI CATATG 1 cut(s) 47
Fnu4HI GCNGC 3 cut(s) 156, 210, 407
FokI GGATG 2 cut(s) 28, 350
FriOI GRGCYC 3 cut(s) 80, 264, 539
Fsp4HI GCNGC 3 cut(s) 156, 210, 407
GluI GCNGC 3 cut(s) 156, 210, 407
HaeIII GGCC 2 cut(s) 78, 360
HgaI GACGC 1 cut(s) 621
Hin1II CATG 2 cut(s) 349, 452
HinfI GANTC 2 cut(s) 331, 454
HphI GGTGA 1 cut(s) 492
Hpy188III TCNNGA 4 cut(s) 95, 234, 328, 335
HpyAV CCTTC 1 cut(s) 293
HpyCH4V TGCA 3 cut(s) 155, 313, 406
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 392
HpyF3I CTNAG 6 cut(s) 227, 232, 380, 387, 429, 524
Hsp92II CATG 2 cut(s) 349, 452
Kzo9I GATC 2 cut(s) 19, 497
LmnI GCTCC 3 cut(s) 267, 534, 594
LpnPI CCDG 6 cut(s) 25, 48, 219, 261, 288, 320
Lsp1109I GCAGC 3 cut(s) 167, 221, 418
LweI GCATC 2 cut(s) 142, 328
MalI GATC 2 cut(s) 21, 499
MboI GATC 2 cut(s) 19, 497
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 3 cut(s) 80, 264, 539
MluCI AATT 6 cut(s) 82, 90, 121, 189, 625, 661
MmeI TCCRAC 2 cut(s) 275, 602
MnlI CCTC 6 cut(s) 67, 130, 170, 234, 513, 602
MseI TTAA 3 cut(s) 41, 89, 111
MslI CAYNNNNRTG 1 cut(s) 614
MspR9I CCNGG 2 cut(s) 13, 276
MvaI CCWGG 2 cut(s) 13, 276
MwoI GCNNNNNNNGC 2 cut(s) 228, 392
NdeI CATATG 1 cut(s) 47
NdeII GATC 2 cut(s) 19, 497
NlaIII CATG 2 cut(s) 349, 452
NlaIV GGNNCC 1 cut(s) 78
NspI RCATGY 1 cut(s) 452
PfeI GAWTC 2 cut(s) 331, 454
PkrI GCNGC 3 cut(s) 157, 211, 408
Psp124BI GAGCTC 2 cut(s) 264, 539
Psp6I CCWGG 2 cut(s) 11, 274
PspGI CCWGG 2 cut(s) 11, 274
PspN4I GGNNCC 1 cut(s) 78
PspOMI GGGCCC 1 cut(s) 76
PspPI GGNCC 3 cut(s) 76, 77, 359
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
RseI CAYNNNNRTG 1 cut(s) 614
SacI GAGCTC 2 cut(s) 264, 539
SaqAI TTAA 3 cut(s) 41, 89, 111
SatI GCNGC 3 cut(s) 156, 210, 407
Sau3AI GATC 2 cut(s) 19, 497
Sau96I GGNCC 3 cut(s) 76, 77, 359
ScrFI CCNGG 2 cut(s) 13, 276
SduI GDGCHC 3 cut(s) 80, 264, 539
SetI ASST 9 cut(s) 130, 218, 233, 264, 305, 327, 426, 505, 539
SfaNI GCATC 2 cut(s) 142, 328
SfcI CTRYAG 2 cut(s) 69, 304
SmiMI CAYNNNNRTG 1 cut(s) 614
Sse9I AATT 6 cut(s) 82, 90, 121, 189, 625, 661
SsiI CCGC 2 cut(s) 516, 532
SstI GAGCTC 2 cut(s) 264, 539
StyD4I CCNGG 2 cut(s) 11, 274
StyI CCWWGG 1 cut(s) 371
TaqI TCGA 1 cut(s) 197
TaqII GACCGA 1 cut(s) 262
TasI AATT 6 cut(s) 82, 90, 121, 189, 625, 661
TatI WGTACW 1 cut(s) 432
TfiI GAWTC 2 cut(s) 331, 454
Tru1I TTAA 3 cut(s) 41, 89, 111
Tru9I TTAA 3 cut(s) 41, 89, 111
TscAI CASTG 1 cut(s) 528
TseI GCWGC 3 cut(s) 155, 209, 406
TspRI CASTG 1 cut(s) 528
XapI RAATTY 3 cut(s) 82, 121, 625
XceI RCATGY 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.