pycom17g01550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004339
Physical Location & Seq
Forward (+)
1035843 .. 1037353
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01550.2

Sequence Viewer

Length: 738 bp
ATGGCCGGATTCTTGGTCTTGGTGGCGGCGGCGTTGGTGGGTCTGTGCATTGATGGCAGTTGGGTGGCGGAGGCGGGTCGGGAGGCTCCGGGAACGACGGCGAGGAGGGTGCACACGCTGTTCTCGGTGGAGTGCCAGAACTACTTCGACTGGCAGACTGTCGGGTTGATGCACAGCTACCGGAAAGCCCGGCAACCTGGCCCGATTACCCGGCTCCTCAGCTGCACCGACGACGAGAAGAAGAAGTACAAAGGGATGCATTTGGCTCCCACTTTTGAGGTTCCTTCCATGAGCAGGCACCCAAAAACTGGCGACTGGTACCCTGCAATTAACAAACCAGCTGGAGTTGTGCACTGGCTTAAGCACAGTAAGGAGGCTGAGAATGTTGATTGGGTTGTGATTCTGGATGCTGACATGATTATCCGTGGGCCGATTGTTCCTTGGGAACTTGGTGCTGAGAAAGGCAAGCCTGTTGCTGCATATTATGGGTACTTGGTTGGATGCGATAATCTGCTTGCTCGACTGCACACAAAGCACCCTGAACTCTGTGATAAAGTTGGTGGGCTTTTGGCCATGCATATAGATGATCTTCGAGCATTGGCACCCGTGTGGCTTTCGAAAACAGAAGAAGTGCGGGAAGATAGAGCTCACTGGACAACTAATATAACTGGTGACATCTATGGGAAATTGGATCGATCAGTGAGATGTATGGATATTCTTTTGGTGCAGCAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00514 map00514
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

27.47

Weight (kDa)

7.68

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 318
AccB1I GGYRCC 3 cut(s) 297, 318, 601
AccB7I CCANNNNNTGG 1 cut(s) 308
AciI CCGC 5 cut(s) 26, 29, 68, 74, 634
AclWI GGATC 1 cut(s) 699
AcoI YGGCCR 2 cut(s) 3, 570
AfaI GTAC 3 cut(s) 248, 320, 491
AfiI CCNNNNNNNGG 2 cut(s) 294, 308
AflII CTTAAG 1 cut(s) 359
AjnI CCWGG 1 cut(s) 196
AleI CACNNNNGTG 1 cut(s) 607
AluBI AGCT 4 cut(s) 177, 222, 341, 647
AluI AGCT 4 cut(s) 177, 222, 341, 647
Alw21I GWGCWC 3 cut(s) 114, 354, 649
Alw44I GTGCAC 2 cut(s) 110, 350
AlwI GGATC 1 cut(s) 699
AoxI GGCC 4 cut(s) 3, 199, 428, 570
ApaLI GTGCAC 2 cut(s) 110, 350
ApeKI GCWGC 3 cut(s) 222, 476, 727
Asp700I GAANNNNTTC 1 cut(s) 143
Asp718I GGTACC 1 cut(s) 318
AspS9I GGNCC 2 cut(s) 200, 428
AsuC2I CCSGG 3 cut(s) 90, 190, 211
AsuHPI GGTGA 1 cut(s) 683
AsuII TTCGAA 1 cut(s) 617
BaeGI GKGCMC 2 cut(s) 114, 354
BalI TGGCCA 1 cut(s) 572
BanI GGYRCC 3 cut(s) 297, 318, 601
BanII GRGCYC 1 cut(s) 649
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
Bbv12I GWGCWC 3 cut(s) 114, 354, 649
BbvCI CCTCAGC 1 cut(s) 218
BbvI GCAGC 2 cut(s) 209, 463
BccI CCATC 1 cut(s) 47
BceAI ACGGC 1 cut(s) 114
BcgI CGANNNNNNTGC 2 cut(s) 91, 125
BciT130I CCWGG 1 cut(s) 198
BcnI CCSGG 3 cut(s) 90, 190, 211
BfrI CTTAAG 1 cut(s) 359
BisI GCNGC 5 cut(s) 27, 30, 223, 477, 728
BlsI GCNGC 5 cut(s) 28, 31, 224, 478, 729
Bme1390I CCNGG 4 cut(s) 90, 190, 198, 211
BmgT120I GGNCC 2 cut(s) 200, 428
BmiI GGNNCC 7 cut(s) 87, 215, 267, 282, 299, 320, 603
BmrFI CCNGG 4 cut(s) 90, 190, 198, 211
BmsI GCATC 4 cut(s) 159, 246, 397, 491
BpmI CTGGAG 1 cut(s) 363
Bpu10I CCTNAGC 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 617
BpuMI CCSGG 3 cut(s) 90, 190, 211
Bsa29I ATCGAT 1 cut(s) 694
BsaJI CCNNGG 2 cut(s) 424, 440
BsaWI WCCGGW 1 cut(s) 180
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 308
Bse1I ACTGG 6 cut(s) 155, 313, 320, 359, 656, 673
BseBI CCWGG 1 cut(s) 198
BseCI ATCGAT 1 cut(s) 694
BseDI CCNNGG 2 cut(s) 424, 440
BseGI GGATG 3 cut(s) 261, 412, 506
BseLI CCNNNNNNNGG 2 cut(s) 294, 308
BseMII CTCAG 3 cut(s) 232, 369, 447
BseNI ACTGG 6 cut(s) 155, 313, 320, 359, 656, 673
BseRI GAGGAG 2 cut(s) 118, 206
BseSI GKGCMC 2 cut(s) 114, 354
BseXI GCAGC 2 cut(s) 209, 463
BsgI GTGCAG 2 cut(s) 208, 509
BshFI GGCC 4 cut(s) 5, 201, 430, 572
BshNI GGYRCC 3 cut(s) 297, 318, 601
BshVI ATCGAT 1 cut(s) 694
BsiHKAI GWGCWC 3 cut(s) 114, 354, 649
BsiSI CCGG 5 cut(s) 6, 89, 181, 190, 211
BslI CCNNNNNNNGG 2 cut(s) 294, 308
BsnI GGCC 4 cut(s) 5, 201, 430, 572
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 3 cut(s) 114, 354, 649
Bsp143I GATC 3 cut(s) 586, 691, 695
BspACI CCGC 5 cut(s) 26, 29, 68, 74, 634
BspANI GGCC 4 cut(s) 5, 201, 430, 572
BspCNI CTCAG 3 cut(s) 231, 370, 448
BspDI ATCGAT 1 cut(s) 694
BspLI GGNNCC 7 cut(s) 87, 215, 267, 282, 299, 320, 603
BspPI GGATC 1 cut(s) 699
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 3 cut(s) 297, 318, 601
BspTI CTTAAG 1 cut(s) 359
BsrI ACTGG 6 cut(s) 155, 313, 320, 359, 656, 673
BssECI CCNNGG 2 cut(s) 424, 440
BssMI GATC 3 cut(s) 586, 691, 695
BssT1I CCWWGG 1 cut(s) 440
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 2 cut(s) 160, 368
BstAFI CTTAAG 1 cut(s) 359
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 3 cut(s) 296, 467, 516
BstDEI CTNAG 3 cut(s) 218, 378, 456
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 3 cut(s) 261, 412, 506
BstKTI GATC 3 cut(s) 589, 694, 698
BstMBI GATC 3 cut(s) 586, 691, 695
BstMWI GCNNNNNNNGC 2 cut(s) 54, 532
BstNI CCWGG 1 cut(s) 198
BstSCI CCNGG 4 cut(s) 88, 188, 196, 209
BstSLI GKGCMC 2 cut(s) 114, 354
BstV1I GCAGC 2 cut(s) 209, 463
Bsu15I ATCGAT 1 cut(s) 694
BsuRI GGCC 4 cut(s) 5, 201, 430, 572
BsuTUI ATCGAT 1 cut(s) 694
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 3 cut(s) 261, 412, 506
BtsIMutI CAGTG 3 cut(s) 352, 649, 705
Cac8I GCNNGC 3 cut(s) 296, 467, 516
Cfr13I GGNCC 2 cut(s) 200, 428
ClaI ATCGAT 1 cut(s) 694
Csp6I GTAC 3 cut(s) 247, 319, 490
CviAII CATG 3 cut(s) 289, 415, 574
CviQI GTAC 3 cut(s) 247, 319, 490
DdeI CTNAG 3 cut(s) 218, 378, 456
DpnI GATC 3 cut(s) 588, 693, 697
DpnII GATC 3 cut(s) 586, 691, 695
EaeI YGGCCR 2 cut(s) 3, 570
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 647
Eco130I CCWWGG 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 649
Eco53kI GAGCTC 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 440
EcoT22I ATGCAT 2 cut(s) 261, 579
EcoT38I GRGCYC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 3 cut(s) 292, 418, 577
FatI CATG 3 cut(s) 288, 414, 573
FauI CCCGC 2 cut(s) 67, 627
Fnu4HI GCNGC 5 cut(s) 27, 30, 223, 477, 728
FokI GGATG 3 cut(s) 268, 419, 513
FriOI GRGCYC 1 cut(s) 649
Fsp4HI GCNGC 5 cut(s) 27, 30, 223, 477, 728
GluI GCNGC 5 cut(s) 27, 30, 223, 477, 728
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 4 cut(s) 5, 201, 430, 572
HapII CCGG 5 cut(s) 6, 89, 181, 190, 211
Hin1II CATG 3 cut(s) 292, 418, 577
HinfI GANTC 2 cut(s) 9, 400
HpaII CCGG 5 cut(s) 6, 89, 181, 190, 211
HphI GGTGA 1 cut(s) 683
Hpy166II GTNNAC 2 cut(s) 112, 352
Hpy188III TCNNGA 2 cut(s) 80, 404
Hpy8I GTNNAC 2 cut(s) 112, 352
Hpy99I CGWCG 3 cut(s) 100, 233, 236
HpyAV CCTTC 1 cut(s) 294
HpyCH4III ACNGT 2 cut(s) 160, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 532
HpyF3I CTNAG 3 cut(s) 218, 378, 456
Hsp92II CATG 3 cut(s) 292, 418, 577
KpnI GGTACC 1 cut(s) 322
Kzo9I GATC 3 cut(s) 586, 691, 695
LmnI GCTCC 3 cut(s) 91, 219, 271
Lsp1109I GCAGC 2 cut(s) 209, 463
LweI GCATC 4 cut(s) 159, 246, 397, 491
MaeIII GTNAC 1 cut(s) 671
MalI GATC 3 cut(s) 588, 693, 697
MboI GATC 3 cut(s) 586, 691, 695
MboII GAAGA 5 cut(s) 250, 253, 581, 638, 650
MhlI GDGCHC 3 cut(s) 114, 354, 649
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 2 cut(s) 327, 686
MluNI TGGCCA 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 478
MnlI CCTC 8 cut(s) 64, 76, 96, 99, 227, 271, 367, 726
Mox20I TGGCCA 1 cut(s) 572
Mph1103I ATGCAT 2 cut(s) 261, 579
MroXI GAANNNNTTC 1 cut(s) 143
MscI TGGCCA 1 cut(s) 572
MseI TTAA 2 cut(s) 330, 360
MslI CAYNNNNRTG 2 cut(s) 582, 607
Msp20I TGGCCA 1 cut(s) 572
MspA1I CMGCKG 2 cut(s) 222, 341
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 5 cut(s) 6, 89, 181, 190, 211
MspR9I CCNGG 4 cut(s) 90, 190, 198, 211
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 54, 532
NciI CCSGG 3 cut(s) 90, 190, 211
NdeII GATC 3 cut(s) 586, 691, 695
NlaIII CATG 3 cut(s) 292, 418, 577
NlaIV GGNNCC 7 cut(s) 87, 215, 267, 282, 299, 320, 603
NmuCI GTSAC 1 cut(s) 671
NsiI ATGCAT 2 cut(s) 261, 579
NspV TTCGAA 1 cut(s) 617
OliI CACNNNNGTG 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 2 cut(s) 9, 400
PflMI CCANNNNNTGG 1 cut(s) 308
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 5 cut(s) 28, 31, 224, 478, 729
Psp124BI GAGCTC 1 cut(s) 649
Psp6I CCWGG 1 cut(s) 196
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 7 cut(s) 87, 215, 267, 282, 299, 320, 603
PspPI GGNCC 2 cut(s) 200, 428
PvuII CAGCTG 2 cut(s) 222, 341
RsaI GTAC 3 cut(s) 248, 320, 491
RsaNI GTAC 3 cut(s) 247, 319, 490
RseI CAYNNNNRTG 2 cut(s) 582, 607
SacI GAGCTC 1 cut(s) 649
SaqAI TTAA 2 cut(s) 330, 360
SatI GCNGC 5 cut(s) 27, 30, 223, 477, 728
Sau3AI GATC 3 cut(s) 586, 691, 695
Sau96I GGNCC 2 cut(s) 200, 428
ScrFI CCNGG 4 cut(s) 90, 190, 198, 211
SduI GDGCHC 3 cut(s) 114, 354, 649
SetI ASST 7 cut(s) 179, 199, 224, 282, 343, 649, 737
SfaNI GCATC 4 cut(s) 159, 246, 397, 491
SfuI TTCGAA 1 cut(s) 617
SmiMI CAYNNNNRTG 2 cut(s) 582, 607
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 2 cut(s) 327, 686
SsiI CCGC 5 cut(s) 26, 29, 68, 74, 634
SstI GAGCTC 1 cut(s) 649
StyD4I CCNGG 4 cut(s) 88, 188, 196, 209
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 2 cut(s) 160, 368
TaqI TCGA 5 cut(s) 147, 520, 592, 617, 694
TasI AATT 2 cut(s) 327, 686
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 29, 32
TfiI GAWTC 2 cut(s) 9, 400
Tru1I TTAA 2 cut(s) 330, 360
Tru9I TTAA 2 cut(s) 330, 360
TscAI CASTG 3 cut(s) 359, 656, 705
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 3 cut(s) 222, 476, 727
Tsp45I GTSAC 1 cut(s) 671
TspGWI ACGGA 1 cut(s) 413
TspRI CASTG 3 cut(s) 359, 656, 705
Van91I CCANNNNNTGG 1 cut(s) 308
Vha464I CTTAAG 1 cut(s) 359
VneI GTGCAC 2 cut(s) 110, 350
XmnI GAANNNNTTC 1 cut(s) 143
Zsp2I ATGCAT 2 cut(s) 261, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.