MD04G1163800.v1.1

lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005189
Physical Location & Seq
Reverse (-)
25490695 .. 25492329
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1163800.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCTTCTCCTAAGCTCTTCTCCCTTCTCATTCTAACTTCTTTGCTCATCGTTTGCGTGTCTTGTTCTTCTTTTGAAGATCAAGGCAGCAAAGTCCCGCCTTCTCCAAAATCCCGAAAACTTATAGAAGCTCCTAGTGCTATTCCTAGGCCTCCTACCAAAGCTAAGAAAGTTGACCTGACTCTGTATTACGAAACACTATGCCCATTCTGTGCACAGTTCATCGTAAATGGACTATCAAAGGTATTCGACACAGATCTCATCAACATTGTGAACTTGAGGCTAGTCCCATATGGAAATGCTCATATACAAGGACCTAACAGCACCATCGTTTGCCAGCACGGCCCGGATGAGTGCTACTTGAACAGCATAGAAGCCTGTGCTATCAACAGCTATCCAGATGCGACGCAGCATTTCAAATTCATTCATTGCATTGAGAACCAAACCATTCAAGGAAAGCAGTCGAAAGATGAATGGAAATCTTGTTCTCAACAACTAGGGATGGATCCGAAACCTGTCCAAGATTGCTACGACAGCGGGTTAGAGAAAAAGCTTATACTACAATATGCAAATGAGACAGCGCATCTTGATCCGGTTCACAACTATGTGCCATGGGTTCTTGTCAATACTAGACCACTCTATGAATCCTACCAAAACTTTGTACCCATTGTCTGTGCGGCTTACAAAGGAAGTCCAAAACCAGAGGCTTGCAAACCACTTCCGCCCAATATCAACTCAACTGATAAGGAAAACTCTACCGGACAAAGATGCTACAAGGGTGAAGAACACCAGGAACAGCTAAAACTTCGTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

272

Amino Acids

30.37

Weight (kDa)

7.48

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 60 - 165 2e-31 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 98, 537, 677, 722
AclWI GGATC 3 cut(s) 500, 513, 584
AcsI RAATTY 1 cut(s) 419
AfaI GTAC 1 cut(s) 663
AgsI TTSAA 4 cut(s) 77, 364, 418, 452
AjnI CCWGG 1 cut(s) 789
AloI GAACNNNNNNTCC 2 cut(s) 469, 501
AluBI AGCT 6 cut(s) 16, 131, 164, 393, 553, 799
AluI AGCT 6 cut(s) 16, 131, 164, 393, 553, 799
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 569
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 3 cut(s) 500, 513, 584
AoxI GGCC 2 cut(s) 149, 343
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 87, 409
ApoI RAATTY 1 cut(s) 419
AspA2I CCTAGG 1 cut(s) 146
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 2 cut(s) 314, 344
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 1 cut(s) 791
AvaII GGWCC 1 cut(s) 314
AvrII CCTAGG 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 99, 421
BccI CCATC 2 cut(s) 335, 496
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 1 cut(s) 791
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 5 cut(s) 135, 147, 284, 497, 630
BglI GCCNNNNNGGC 1 cut(s) 342
BglII AGATCT 1 cut(s) 256
BisI GCNGC 3 cut(s) 88, 410, 678
BlnI CCTAGG 1 cut(s) 146
BlsI GCNGC 3 cut(s) 89, 411, 679
Bme1390I CCNGG 2 cut(s) 347, 791
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 314, 344
BmiI GGNNCC 1 cut(s) 507
BmrFI CCNGG 2 cut(s) 347, 791
BmsI GCATC 3 cut(s) 391, 592, 758
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 146, 611
BsaWI WCCGGW 2 cut(s) 592, 758
Bse3DI GCAATG 1 cut(s) 427
BseBI CCWGG 1 cut(s) 791
BseDI CCNNGG 2 cut(s) 146, 611
BseGI GGATG 2 cut(s) 355, 507
BseMI GCAATG 1 cut(s) 427
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 99, 421
BshFI GGCC 2 cut(s) 151, 345
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 3 cut(s) 347, 593, 759
BslFI GGGAC 2 cut(s) 80, 272
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 2 cut(s) 80, 272
BsnI GGCC 2 cut(s) 151, 345
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 4 cut(s) 79, 256, 505, 589
Bsp19I CCATGG 1 cut(s) 611
BspACI CCGC 4 cut(s) 98, 537, 677, 722
BspANI GGCC 2 cut(s) 151, 345
BspLI GGNNCC 1 cut(s) 507
BspPI GGATC 3 cut(s) 500, 513, 584
BspQI GCTCTTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 427
BssECI CCNNGG 2 cut(s) 146, 611
BssMI GATC 4 cut(s) 79, 256, 505, 589
BssT1I CCWWGG 2 cut(s) 146, 611
Bst2UI CCWGG 1 cut(s) 791
Bst4CI ACNGT 1 cut(s) 219
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 2 cut(s) 338, 709
BstDEI CTNAG 2 cut(s) 12, 165
BstDSI CCRYGG 1 cut(s) 611
BstF5I GGATG 2 cut(s) 355, 507
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 4 cut(s) 82, 259, 508, 592
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 4 cut(s) 79, 256, 505, 589
BstMWI GCNNNNNNNGC 3 cut(s) 137, 342, 534
BstNI CCWGG 1 cut(s) 791
BstSCI CCNGG 2 cut(s) 345, 789
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 99, 421
BstX2I RGATCY 2 cut(s) 256, 505
BstYI RGATCY 2 cut(s) 256, 505
BsuRI GGCC 2 cut(s) 151, 345
BtgI CCRYGG 1 cut(s) 611
BtsCI GGATG 2 cut(s) 355, 507
Cac8I GCNNGC 2 cut(s) 338, 709
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 2 cut(s) 314, 344
CseI GACGC 1 cut(s) 415
Csp6I GTAC 1 cut(s) 662
CviAII CATG 1 cut(s) 612
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 2 cut(s) 12, 165
DpnI GATC 4 cut(s) 81, 258, 507, 591
DpnII GATC 4 cut(s) 79, 256, 505, 589
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 711
Eco130I CCWWGG 2 cut(s) 146, 611
Eco147I AGGCCT 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 789
EcoT14I CCWWGG 2 cut(s) 146, 611
ErhI CCWWGG 2 cut(s) 146, 611
FaeI CATG 1 cut(s) 615
FaqI GGGAC 2 cut(s) 80, 272
FatI CATG 1 cut(s) 611
FauI CCCGC 2 cut(s) 105, 530
FauNDI CATATG 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 88, 410, 678
FokI GGATG 2 cut(s) 362, 514
Fsp4HI GCNGC 3 cut(s) 88, 410, 678
FspBI CTAG 5 cut(s) 135, 147, 284, 497, 630
GlaI GCGC 1 cut(s) 582
GluI GCNGC 3 cut(s) 88, 410, 678
HaeIII GGCC 2 cut(s) 151, 345
HapII CCGG 3 cut(s) 347, 593, 759
HgaI GACGC 1 cut(s) 415
HhaI GCGC 1 cut(s) 583
Hin1II CATG 1 cut(s) 615
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 2 cut(s) 181, 644
HpaII CCGG 3 cut(s) 347, 593, 759
HphI GGTGA 1 cut(s) 791
Hpy166II GTNNAC 4 cut(s) 175, 215, 274, 598
Hpy188I TCNGA 1 cut(s) 510
Hpy188III TCNNGA 3 cut(s) 114, 398, 587
Hpy8I GTNNAC 4 cut(s) 175, 215, 274, 598
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 2 cut(s) 35, 111
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4V TGCA 4 cut(s) 215, 432, 569, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 342, 534
HpyF3I CTNAG 2 cut(s) 12, 165
Hsp92II CATG 1 cut(s) 615
HspAI GCGC 1 cut(s) 581
Kzo9I GATC 4 cut(s) 79, 256, 505, 589
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 2 cut(s) 99, 421
LweI GCATC 3 cut(s) 391, 592, 758
MaeI CTAG 5 cut(s) 135, 147, 284, 497, 630
MalI GATC 4 cut(s) 81, 258, 507, 591
MboI GATC 4 cut(s) 79, 256, 505, 589
MboII GAAGA 4 cut(s) 10, 60, 89, 794
MflI RGATCY 2 cut(s) 256, 505
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 1 cut(s) 419
MlyI GAGTC 1 cut(s) 175
MnlI CCTC 3 cut(s) 162, 273, 697
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 537
MspI CCGG 3 cut(s) 347, 593, 759
MspR9I CCNGG 2 cut(s) 347, 791
MvaI CCWGG 1 cut(s) 791
MwoI GCNNNNNNNGC 3 cut(s) 137, 342, 534
NciI CCSGG 1 cut(s) 347
NcoI CCATGG 1 cut(s) 611
NdeI CATATG 1 cut(s) 292
NdeII GATC 4 cut(s) 79, 256, 505, 589
NlaIII CATG 1 cut(s) 615
NlaIV GGNNCC 1 cut(s) 507
PceI AGGCCT 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 644
PkrI GCNGC 3 cut(s) 89, 411, 679
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
PpuMI RGGWCCY 1 cut(s) 314
Psp5II RGGWCCY 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 789
PspGI CCWGG 1 cut(s) 789
PspN4I GGNNCC 1 cut(s) 507
PspPI GGNCC 2 cut(s) 314, 344
PspPPI RGGWCCY 1 cut(s) 314
PsuI RGATCY 2 cut(s) 256, 505
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 603
SapI GCTCTTC 1 cut(s) 23
SatI GCNGC 3 cut(s) 88, 410, 678
Sau3AI GATC 4 cut(s) 79, 256, 505, 589
Sau96I GGNCC 2 cut(s) 314, 344
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 347, 791
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 3 cut(s) 391, 592, 758
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
Sse9I AATT 1 cut(s) 419
SseBI AGGCCT 1 cut(s) 151
SsiI CCGC 4 cut(s) 98, 537, 677, 722
SspMI CTAG 5 cut(s) 135, 147, 284, 497, 630
StuI AGGCCT 1 cut(s) 151
StyD4I CCNGG 2 cut(s) 345, 789
StyI CCWWGG 2 cut(s) 146, 611
TaaI ACNGT 1 cut(s) 219
TaqI TCGA 2 cut(s) 249, 464
TasI AATT 1 cut(s) 419
TauI GCSGC 1 cut(s) 680
TfiI GAWTC 1 cut(s) 644
TseI GCWGC 2 cut(s) 87, 409
TspDTI ATGAA 5 cut(s) 211, 412, 416, 486, 657
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 1 cut(s) 419
XmaJI CCTAGG 1 cut(s) 146
XspI CTAG 5 cut(s) 135, 147, 284, 497, 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.