RchiOBHm_Chr3g0462161
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0005189
Physical Location & Seq
Forward (+)
9936874 .. 9938970
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42855

Sequence Viewer

Length: 750 bp
ATGGCTTCTCATAAGTTCTTCTCCATCACTGTTCTAACAGCTTTGCTCTCGCTCTGCACCAATGCTCTGTCTCGTACGGAATACAATTGCTCTTCTCAGAAAGTTAACCTAACTATATATTATGACACTCTTAGCCCGTACTGTGAGGAATTCATTGTATATGAGCTTCCAAAGGCATTTGAGCTGGAACTCATGCACATTATCAACCTCCGTCTAGTACCATCTGGAAATGCTTACATCCAAGAACCTAACAACACCATCAGTTGCCAGGATGGCCCAGCTGAATGCTACTTGAACAGTATAGAAGCTTGTGTAATCAGCATCTGGCCGGATGTGAATAAACATTTCAAATTTATTCACTGTGTTGCATGGCAAAACTTGGGACGATTACAGTTAAAAGAAGATGCATGGAAATCATGTTCTAAAATAACGATGCTGAGTGACAAACCTGTCTCAAATTGCTACAAAAGCGGATATGCGAAAAAGCTTCTACTACGATACGCTAATGAAACAAAGCACCTAGATCCTCCTCATACAAATGTGCCATGGGTTGTCATCAATAAGCGACCACTATACTATGACTACAAGAACTTTGTAAGCTACGTCTGTGAGGCTTACCAAGGCCATCCTAAACCATCGGCTTGCACAACACCTATCTTCAGCTCAGCTAAAAAGTCAAACTCAACCCGCTTGCATATATATTATTATCTGCTGCACCTTATGTCCTGTGCAGCTTTCCTAATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

249

Amino Acids

28.58

Weight (kDa)

8.51

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 36 - 142 9.4e-26 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 449
AciI CCGC 2 cut(s) 471, 688
AclWI GGATC 1 cut(s) 518
AcoI YGGCCR 1 cut(s) 326
AcsI RAATTY 2 cut(s) 149, 350
AcuI CTGAAG 1 cut(s) 643
AfaI GTAC 3 cut(s) 76, 140, 219
AgsI TTSAA 2 cut(s) 295, 349
AjnI CCWGG 1 cut(s) 267
AloI GAACNNNNNNTCC 2 cut(s) 403, 435
Alw26I GTCTC 2 cut(s) 75, 457
AlwI GGATC 1 cut(s) 518
AlwNI CAGNNNCTG 1 cut(s) 324
AoxI GGCC 3 cut(s) 274, 326, 622
ApeKI GCWGC 2 cut(s) 712, 731
ApoI RAATTY 2 cut(s) 149, 350
AspS9I GGNCC 1 cut(s) 275
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BbvI GCAGC 2 cut(s) 699, 743
BccI CCATC 6 cut(s) 32, 229, 266, 266, 633, 643
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 75, 457
BfaI CTAG 3 cut(s) 215, 521, 748
BglI GCCNNNNNGGC 1 cut(s) 273
BisI GCNGC 2 cut(s) 713, 732
BlpI GCTNAGC 1 cut(s) 664
BlsI GCNGC 2 cut(s) 714, 733
Bme1390I CCNGG 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 3 cut(s) 330, 394, 423
Bpu1102I GCTNAGC 1 cut(s) 664
BsaJI CCNNGG 2 cut(s) 545, 619
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 545, 619
BseGI GGATG 4 cut(s) 237, 277, 337, 625
BseMII CTCAG 3 cut(s) 110, 428, 678
BseRI GAGGAG 1 cut(s) 519
BseXI GCAGC 2 cut(s) 699, 743
BseYI CCCAGC 1 cut(s) 277
BsgI GTGCAG 3 cut(s) 40, 698, 750
BshFI GGCC 3 cut(s) 276, 328, 624
BsiSI CCGG 1 cut(s) 329
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 396
BsmAI GTCTC 2 cut(s) 75, 457
BsmFI GGGAC 1 cut(s) 396
BsmI GAATGC 1 cut(s) 290
BsnI GGCC 3 cut(s) 276, 328, 624
Bsp143I GATC 1 cut(s) 523
Bsp1720I GCTNAGC 1 cut(s) 664
Bsp19I CCATGG 1 cut(s) 545
BspACI CCGC 2 cut(s) 471, 688
BspANI GGCC 3 cut(s) 276, 328, 624
BspCNI CTCAG 3 cut(s) 109, 429, 677
BspPI GGATC 1 cut(s) 518
BspQI GCTCTTC 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 545, 619
BssMI GATC 1 cut(s) 523
BssT1I CCWWGG 2 cut(s) 545, 619
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 5 cut(s) 31, 143, 299, 362, 393
Bst6I CTCTTC 1 cut(s) 97
BstC8I GCNNGC 2 cut(s) 643, 692
BstDEI CTNAG 4 cut(s) 96, 131, 437, 664
BstDSI CCRYGG 1 cut(s) 545
BstF5I GGATG 4 cut(s) 237, 277, 337, 625
BstKTI GATC 1 cut(s) 526
BstMAI GTCTC 2 cut(s) 75, 457
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 2 cut(s) 273, 468
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 267
BstV1I GCAGC 2 cut(s) 699, 743
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 3 cut(s) 276, 328, 624
BtgI CCRYGG 1 cut(s) 545
BtsCI GGATG 4 cut(s) 237, 277, 337, 625
BtsIMutI CAGTG 2 cut(s) 27, 358
Cac8I GCNNGC 2 cut(s) 643, 692
CaiI CAGNNNCTG 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 3 cut(s) 75, 139, 218
CviAII CATG 5 cut(s) 193, 369, 408, 417, 546
CviQI GTAC 3 cut(s) 75, 139, 218
DdeI CTNAG 4 cut(s) 96, 131, 437, 664
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
DrdI GACNNNNNNGTC 1 cut(s) 449
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 97
EarI CTCTTC 1 cut(s) 97
Eco130I CCWWGG 2 cut(s) 545, 619
Eco57I CTGAAG 1 cut(s) 643
EcoRI GAATTC 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 2 cut(s) 545, 619
EcoT22I ATGCAT 1 cut(s) 409
ErhI CCWWGG 2 cut(s) 545, 619
FaeI CATG 5 cut(s) 196, 372, 411, 420, 549
FaqI GGGAC 1 cut(s) 396
FatI CATG 5 cut(s) 192, 368, 407, 416, 545
FauI CCCGC 1 cut(s) 695
Fnu4HI GCNGC 2 cut(s) 713, 732
FokI GGATG 4 cut(s) 224, 284, 344, 612
Fsp4HI GCNGC 2 cut(s) 713, 732
FspBI CTAG 3 cut(s) 215, 521, 748
GluI GCNGC 2 cut(s) 713, 732
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 3 cut(s) 276, 328, 624
HapII CCGG 1 cut(s) 329
Hin1II CATG 5 cut(s) 196, 372, 411, 420, 549
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 2 cut(s) 306, 485
HpaI GTTAAC 1 cut(s) 106
HpaII CCGG 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 99
Hpy188III TCNNGA 1 cut(s) 225
Hpy8I GTNNAC 1 cut(s) 106
HpyCH4III ACNGT 5 cut(s) 31, 143, 299, 362, 393
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 8 cut(s) 57, 196, 368, 407, 645, 694, 715, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 273, 468
HpyF3I CTNAG 4 cut(s) 96, 131, 437, 664
HpySE526I ACGT 1 cut(s) 603
Hsp92II CATG 5 cut(s) 196, 372, 411, 420, 549
KspAI GTTAAC 1 cut(s) 106
Kzo9I GATC 1 cut(s) 523
LguI GCTCTTC 1 cut(s) 97
LpnPI CCDG 9 cut(s) 170, 210, 254, 281, 291, 310, 342, 462, 739
Lsp1109I GCAGC 2 cut(s) 699, 743
LweI GCATC 3 cut(s) 330, 394, 423
MaeI CTAG 3 cut(s) 215, 521, 748
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 1 cut(s) 440
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 4 cut(s) 10, 84, 413, 649
MfeI CAATTG 1 cut(s) 85
MflI RGATCY 1 cut(s) 523
MluCI AATT 4 cut(s) 85, 149, 350, 457
MnlI CCTC 5 cut(s) 139, 218, 537, 540, 604
Mph1103I ATGCAT 1 cut(s) 409
MseI TTAA 2 cut(s) 105, 395
MslI CAYNNNNRTG 1 cut(s) 537
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 269
MunI CAATTG 1 cut(s) 85
Mva1269I GAATGC 1 cut(s) 290
MvaI CCWGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 273, 468
NcoI CCATGG 1 cut(s) 545
NdeII GATC 1 cut(s) 523
NlaIII CATG 5 cut(s) 196, 372, 411, 420, 549
NmuCI GTSAC 1 cut(s) 440
NsiI ATGCAT 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 97
PctI GAATGC 1 cut(s) 290
Pfl23II CGTACG 1 cut(s) 74
PkrI GCNGC 2 cut(s) 714, 733
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 277
PspGI CCWGG 1 cut(s) 267
PspLI CGTACG 1 cut(s) 74
PspPI GGNCC 1 cut(s) 275
PstNI CAGNNNCTG 1 cut(s) 324
PsuI RGATCY 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 3 cut(s) 76, 140, 219
RsaNI GTAC 3 cut(s) 75, 139, 218
RseI CAYNNNNRTG 1 cut(s) 537
SapI GCTCTTC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 105, 395
SatI GCNGC 2 cut(s) 713, 732
Sau3AI GATC 1 cut(s) 523
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 269
SfaNI GCATC 3 cut(s) 330, 394, 423
SmiMI CAYNNNNRTG 1 cut(s) 537
Sse9I AATT 4 cut(s) 85, 149, 350, 457
SsiI CCGC 2 cut(s) 471, 688
SspMI CTAG 3 cut(s) 215, 521, 748
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 545, 619
TaaI ACNGT 5 cut(s) 31, 143, 299, 362, 393
TaiI ACGT 1 cut(s) 606
TasI AATT 4 cut(s) 85, 149, 350, 457
Tru1I TTAA 2 cut(s) 105, 395
Tru9I TTAA 2 cut(s) 105, 395
TscAI CASTG 2 cut(s) 34, 365
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 712, 731
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 2 cut(s) 142, 522
TspGWI ACGGA 2 cut(s) 92, 200
TspRI CASTG 2 cut(s) 34, 365
XapI RAATTY 2 cut(s) 149, 350
XspI CTAG 3 cut(s) 215, 521, 748
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.