Rorug03G0054700
ERF Family

Gamma-interferon-inducible lysosomal thiol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005189
Physical Location & Seq
Reverse (-)
4211218 .. 4212915
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0054700.1

Sequence Viewer

Length: 1698 bp
ATGAAGAAACACTACAACACCATTCTCTCCCTACTAGAAAAATGTAAAACCATGAGTGAATTGAAAGAGATACATGGGATGATGATCACTACCTCAGTTGATAAGAATGTAATCCCATTGAGTAGGCTTATTGATTTCTGTGCTAATTCTGAAGCTGGAGACATCAGCTATGCCAAGTCGGTTTTTCGCCGAATTGAGCAACCTAGTGTCTATATCTGGAACTCAATGATCAGAGGGTGTTCGAATAGCGAGAACCCAGTTGAGTCTTTGATAATGTATAGAGAAATGGTGCATAGGGGTTATGCCCCTGACCATTTTACATTTCCATTTGTGCTCAAAGGGTGTTCGATAATCAGTGATGTTCATTATGGGAAATGTGTTCATAACTGCATTGTGAAAACTGGGTTTGAGTTGAATGTGTATGCCTCTTCTGGTTTACTCAATATGTATGGTTTTTGTGCAGATATGGAATCTGCACAGAAGGTGTTTGATAAAATGCCTAAGTGGAATGTAGTTGCTTGGACTAGTCTGATTTCCGGGTTTGTTAGGAACAATCAAGCTAGTGAGGCTATGAAGGTGTTTAAGGATATGGAACTTTGTAATGTCGAGGCTAATGAGATTACGATGGTCAATGTCTTAGTTGCCTGTGCTCGAAGTAGGGATATCGATACTGGGAAGTGGGTTCACAGTCGTATTCGCCAACTTGGGTTTGATCCGTTTGAATCGAGTTTTGATTTTAATGTCATTCTTGCAACGGCCATTGTAGATATGTATGCAAAATGTGGCAGCTTGAGAACAGCAAGGCATCTGTTTGACAAGATGCCTGACAGAAACTTGGTTGCCTGGAATTCCATGATCAGTGCCTACAATCAATATGGCCAGGGTGATAAAGCGATTGATCTCTTTTTTGATATGCGGATTGCTGGGTTTGTTCCGGACCAAGCTACCATACTGGGTGTGACTGGTTCTTGTGCGCAGTTGGGGGCTTTGGCATTGGGACAAAGTCTTCATTCGTATGTATCAAAAACTAGCATAGGCACAGATGCTTCTATTGGAACTGCTTTAGTGGACATGTATACGAAAAATGGAGATGCAGGCAGTGCACAAAGAATTTTTGACAATATGCAGAAGAAAGATGTAATGGCGTGGACTAGTATGATTGTTGGTTTAGCTATGCATGGGCATGCCGTGGAAGCACTGAATACTTTTAGAAGAATGCAAGAGGATGCTAGTGTTATTCCAGACCAGATCACTTATATTGGGGTTTTATGCGCGTGTAGCCATGTTGGGCTTGTGGAGGAGGGTCAGAGACACTTTGGCTCAATGGTTGATGTTTATGGCATAAAACCAACTGCAGAGCATTACGGCTGCATGGTTGATCTTCTGAGTCGAGCAGGCCGGTTTGAAGAGGCGGTGAAACTACTGGAAGAAATGCCAATTCAACCCAATGTTGCTATATGGGGAGCTCTCTTAAATGGGTGTGAGATATATGAAAATGTAGATCTAGCTGACCAAGTTAGAAGATACATAACAGAGTTGGAGCCTCATGTTAGTGGAGTTTTCGTCCTTCTTTCCAATATATATGCTAGGGCCGGAAGATGGCAAGAAGTAAAGCTGACTAGAGAATTGATGAAAGATAGGAGGATTGCCAAAAGTCTTGGATGTAGTTCAGTTGAAATGAAATTGTTAAGTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

565

Amino Acids

63.1

Weight (kDa)

6.33

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 68 - 115 6.2e-09 PPR repeat family
PPR_2 PF13041 170 - 217 1.6e-09 PPR repeat family
PPR PF01535 172 - 201 6.2e-07 PPR repeat
PPR_2 PF13041 278 - 316 2.1e-07 PPR repeat family
PPR PF01535 280 - 309 1.4e-06 PPR repeat
PPR_1 PF12854 447 - 478 4.8e-06 PPR repeat
E_motif PF20431 497 - 559 5.2e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 975
AccI GTMKAC 1 cut(s) 1076
AccII CGCG 1 cut(s) 1274
AccIII TCCGGA 1 cut(s) 934
AciI CCGC 2 cut(s) 916, 1412
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 2 cut(s) 756, 877
AcsI RAATTY 2 cut(s) 847, 1110
AcuI CTGAAG 1 cut(s) 171
AfiI CCNNNNNNNGG 1 cut(s) 1599
AflIII ACRYGT 1 cut(s) 1071
AgsI TTSAA 6 cut(s) 64, 415, 722, 1406, 1442, 1676
AhlI ACTAGT 2 cut(s) 524, 1151
AjnI CCWGG 2 cut(s) 842, 879
AluBI AGCT 9 cut(s) 155, 168, 560, 789, 944, 1172, 1466, 1508, 1615
AluI AGCT 9 cut(s) 155, 168, 560, 789, 944, 1172, 1466, 1508, 1615
Alw21I GWGCWC 4 cut(s) 336, 652, 1105, 1468
Alw26I GTCTC 2 cut(s) 153, 1303
Alw44I GTGCAC 1 cut(s) 1101
AlwI GGATC 1 cut(s) 707
Aor13HI TCCGGA 1 cut(s) 934
AoxI GGCC 4 cut(s) 756, 877, 1396, 1590
ApaLI GTGCAC 1 cut(s) 1101
ApeKI GCWGC 2 cut(s) 786, 1368
ApoI RAATTY 2 cut(s) 847, 1110
AspLEI GCGC 2 cut(s) 976, 1274
AspS9I GGNCC 2 cut(s) 937, 1590
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 2 cut(s) 896, 1426
AsuII TTCGAA 1 cut(s) 242
AvaII GGWCC 1 cut(s) 937
BaeGI GKGCMC 1 cut(s) 1105
BalI TGGCCA 1 cut(s) 879
BanII GRGCYC 1 cut(s) 1468
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 998
Bbv12I GWGCWC 4 cut(s) 336, 652, 1105, 1468
BbvI GCAGC 2 cut(s) 798, 1355
BccI CCATC 2 cut(s) 619, 1593
BceAI ACGGC 3 cut(s) 771, 1172, 1381
BciT130I CCWGG 2 cut(s) 844, 881
BclI TGATCA 3 cut(s) 84, 228, 855
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 2 cut(s) 153, 1303
BcuI ACTAGT 2 cut(s) 524, 1151
BfmI CTRYAG 1 cut(s) 1353
BglII AGATCT 1 cut(s) 1501
BisI GCNGC 2 cut(s) 787, 1369
BlsI GCNGC 2 cut(s) 788, 1370
Bme1390I CCNGG 3 cut(s) 538, 844, 881
Bme18I GGWCC 1 cut(s) 937
BmgT120I GGNCC 2 cut(s) 937, 1590
BmiI GGNNCC 1 cut(s) 1542
BmrFI CCNGG 3 cut(s) 538, 844, 881
BmrI ACTGGG 4 cut(s) 251, 411, 681, 962
BmsI GCATC 5 cut(s) 810, 814, 1033, 1081, 1216
BmuI ACTGGG 4 cut(s) 251, 411, 681, 962
BpiI GAAGAC 1 cut(s) 998
BpmI CTGGAG 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 811
BpuMI CCSGG 1 cut(s) 538
Bsa29I ATCGAT 1 cut(s) 666
BsaBI GATNNNNATC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 880, 1188
BsaWI WCCGGW 1 cut(s) 934
BsaXI ACNNNNNCTCC 2 cut(s) 1548, 1578
Bsc4I CCNNNNNNNGG 1 cut(s) 1599
Bse118I RCCGGY 1 cut(s) 1398
Bse1I ACTGG 6 cut(s) 257, 406, 676, 957, 967, 1428
Bse8I GATNNNNATC 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 934
BseBI CCWGG 2 cut(s) 844, 881
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 2 cut(s) 880, 1188
BseGI GGATG 3 cut(s) 84, 1231, 1667
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 1 cut(s) 1599
BseMII CTCAG 2 cut(s) 108, 1376
BseNI ACTGG 6 cut(s) 257, 406, 676, 957, 967, 1428
BseRI GAGGAG 1 cut(s) 1313
BseSI GKGCMC 1 cut(s) 1105
BseXI GCAGC 2 cut(s) 798, 1355
BseYI CCCAGC 1 cut(s) 923
BsgI GTGCAG 2 cut(s) 459, 480
Bsh1236I CGCG 1 cut(s) 1274
BshFI GGCC 4 cut(s) 758, 879, 1398, 1592
BshVI ATCGAT 1 cut(s) 666
BsiHKAI GWGCWC 4 cut(s) 336, 652, 1105, 1468
BsiSI CCGG 4 cut(s) 537, 935, 1399, 1593
BslFI GGGAC 1 cut(s) 1011
BslI CCNNNNNNNGG 1 cut(s) 1599
BsmAI GTCTC 2 cut(s) 153, 1303
BsmFI GGGAC 1 cut(s) 1011
BsmI GAATGC 1 cut(s) 1221
BsnI GGCC 4 cut(s) 758, 879, 1398, 1592
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 336, 652, 1105, 1468
Bsp13I TCCGGA 1 cut(s) 934
Bsp143I GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
BspACI CCGC 2 cut(s) 916, 1412
BspANI GGCC 4 cut(s) 758, 879, 1398, 1592
BspCNI CTCAG 2 cut(s) 107, 1377
BspDI ATCGAT 1 cut(s) 666
BspEI TCCGGA 1 cut(s) 934
BspFNI CGCG 1 cut(s) 1274
BspLI GGNNCC 1 cut(s) 1542
BspMAI CTGCAG 1 cut(s) 1357
BspPI GGATC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 1398
BsrI ACTGG 6 cut(s) 257, 406, 676, 957, 967, 1428
BssAI RCCGGY 1 cut(s) 1398
BssECI CCNNGG 2 cut(s) 880, 1188
BssMI GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
BssNAI GTATAC 1 cut(s) 1077
Bst1107I GTATAC 1 cut(s) 1077
Bst2UI CCWGG 2 cut(s) 844, 881
Bst4CI ACNGT 1 cut(s) 689
Bst6I CTCTTC 2 cut(s) 433, 1401
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 1096, 1185, 1396
BstDEI CTNAG 4 cut(s) 94, 501, 637, 1385
BstDSI CCRYGG 1 cut(s) 1188
BstF5I GGATG 3 cut(s) 84, 1231, 1667
BstFNI CGCG 1 cut(s) 1274
BstHHI GCGC 2 cut(s) 976, 1274
BstKTI GATC 8 cut(s) 87, 231, 715, 858, 901, 1251, 1381, 1504
BstMAI GTCTC 2 cut(s) 153, 1303
BstMBI GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
BstMWI GCNNNNNNNGC 4 cut(s) 566, 1100, 1193, 1278
BstNI CCWGG 2 cut(s) 844, 881
BstNSI RCATGY 2 cut(s) 1075, 1187
BstSCI CCNGG 3 cut(s) 536, 842, 879
BstSFI CTRYAG 1 cut(s) 1353
BstSLI GKGCMC 1 cut(s) 1105
BstUI CGCG 1 cut(s) 1274
BstV1I GCAGC 2 cut(s) 798, 1355
BstV2I GAAGAC 1 cut(s) 998
BstX2I RGATCY 1 cut(s) 1501
BstYI RGATCY 1 cut(s) 1501
BstZ17I GTATAC 1 cut(s) 1077
Bsu15I ATCGAT 1 cut(s) 666
BsuRI GGCC 4 cut(s) 758, 879, 1398, 1592
BsuTUI ATCGAT 1 cut(s) 666
BtgI CCRYGG 1 cut(s) 1188
BtsCI GGATG 3 cut(s) 84, 1231, 1667
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 4 cut(s) 361, 865, 1105, 1196
Cac8I GCNNGC 3 cut(s) 1096, 1185, 1396
CfoI GCGC 2 cut(s) 976, 1274
Cfr10I RCCGGY 1 cut(s) 1398
Cfr13I GGNCC 2 cut(s) 937, 1590
ClaI ATCGAT 1 cut(s) 666
CviAII CATG 9 cut(s) 52, 74, 853, 1072, 1178, 1184, 1283, 1372, 1547
DdeI CTNAG 4 cut(s) 94, 501, 637, 1385
DpnI GATC 8 cut(s) 86, 230, 714, 857, 900, 1250, 1380, 1503
DpnII GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
EaeI YGGCCR 2 cut(s) 756, 877
Eam1104I CTCTTC 2 cut(s) 433, 1401
EarI CTCTTC 2 cut(s) 433, 1401
Ecl136II GAGCTC 1 cut(s) 1466
Eco24I GRGCYC 1 cut(s) 1468
Eco32I GATATC 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 937
Eco53kI GAGCTC 1 cut(s) 1466
Eco57I CTGAAG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 1466
EcoRI GAATTC 1 cut(s) 847
EcoRII CCWGG 2 cut(s) 842, 879
EcoRV GATATC 1 cut(s) 664
EcoT22I ATGCAT 1 cut(s) 1179
EcoT38I GRGCYC 1 cut(s) 1468
FaeI CATG 9 cut(s) 55, 77, 856, 1075, 1181, 1187, 1286, 1375, 1550
FaqI GGGAC 1 cut(s) 1011
FatI CATG 9 cut(s) 51, 73, 852, 1071, 1177, 1183, 1282, 1371, 1546
FbaI TGATCA 3 cut(s) 84, 228, 855
FblI GTMKAC 1 cut(s) 1076
Fnu4HI GCNGC 2 cut(s) 787, 1369
FokI GGATG 3 cut(s) 91, 1238, 1674
FriOI GRGCYC 1 cut(s) 1468
Fsp4HI GCNGC 2 cut(s) 787, 1369
FspI TGCGCA 1 cut(s) 975
GlaI GCGC 2 cut(s) 975, 1273
GluI GCNGC 2 cut(s) 787, 1369
GsaI CCCAGC 1 cut(s) 927
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 4 cut(s) 758, 879, 1398, 1592
HapII CCGG 4 cut(s) 537, 935, 1399, 1593
HhaI GCGC 2 cut(s) 976, 1274
Hin1II CATG 9 cut(s) 55, 77, 856, 1075, 1181, 1187, 1286, 1375, 1550
Hin6I GCGC 2 cut(s) 974, 1272
HinP1I GCGC 2 cut(s) 974, 1272
HinfI GANTC 4 cut(s) 263, 470, 722, 1387
HpaII CCGG 4 cut(s) 537, 935, 1399, 1593
HphI GGTGA 2 cut(s) 896, 1426
Hpy166II GTNNAC 6 cut(s) 437, 685, 1069, 1077, 1103, 1149
Hpy188I TCNGA 5 cut(s) 151, 233, 531, 1308, 1386
Hpy188III TCNNGA 3 cut(s) 217, 935, 1241
Hpy8I GTNNAC 6 cut(s) 437, 685, 1069, 1077, 1103, 1149
HpyAV CCTTC 3 cut(s) 475, 568, 1577
HpyCH4III ACNGT 1 cut(s) 689
HpyF10VI GCNNNNNNNGC 4 cut(s) 566, 1100, 1193, 1278
HpyF3I CTNAG 4 cut(s) 94, 501, 637, 1385
Hsp92II CATG 9 cut(s) 55, 77, 856, 1075, 1181, 1187, 1286, 1375, 1550
HspAI GCGC 2 cut(s) 974, 1272
Kpn2I TCCGGA 1 cut(s) 934
Ksp22I TGATCA 3 cut(s) 84, 228, 855
Kzo9I GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
LmnI GCTCC 2 cut(s) 1463, 1540
Lsp1109I GCAGC 2 cut(s) 798, 1355
LweI GCATC 5 cut(s) 810, 814, 1033, 1081, 1216
MaeIII GTNAC 1 cut(s) 958
MalI GATC 8 cut(s) 86, 230, 714, 857, 900, 1250, 1380, 1503
MboI GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
MflI RGATCY 1 cut(s) 1501
MhlI GDGCHC 4 cut(s) 336, 652, 1105, 1468
MlsI TGGCCA 1 cut(s) 879
MluCI AATT 8 cut(s) 59, 145, 192, 847, 1110, 1437, 1625, 1682
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 2 cut(s) 272, 1396
MmeI TCCRAC 1 cut(s) 1518
Mox20I TGGCCA 1 cut(s) 879
Mph1103I ATGCAT 1 cut(s) 1179
MroI TCCGGA 1 cut(s) 934
MscI TGGCCA 1 cut(s) 879
MseI TTAA 4 cut(s) 582, 738, 1472, 1688
MslI CAYNNNNRTG 3 cut(s) 1014, 1182, 1551
Msp20I TGGCCA 1 cut(s) 879
MspI CCGG 4 cut(s) 537, 935, 1399, 1593
MspR9I CCNGG 3 cut(s) 538, 844, 881
Mva1269I GAATGC 1 cut(s) 1221
MvaI CCWGG 2 cut(s) 844, 881
MvnI CGCG 1 cut(s) 1274
MwoI GCNNNNNNNGC 4 cut(s) 566, 1100, 1193, 1278
NciI CCSGG 1 cut(s) 538
NdeII GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
NlaIII CATG 9 cut(s) 55, 77, 856, 1075, 1181, 1187, 1286, 1375, 1550
NlaIV GGNNCC 1 cut(s) 1542
NmuCI GTSAC 1 cut(s) 958
NsbI TGCGCA 1 cut(s) 975
NsiI ATGCAT 1 cut(s) 1179
NspI RCATGY 2 cut(s) 1075, 1187
NspV TTCGAA 1 cut(s) 242
PaeI GCATGC 1 cut(s) 1187
PciI ACATGT 1 cut(s) 1071
PctI GAATGC 1 cut(s) 1221
PfeI GAWTC 2 cut(s) 470, 722
PflFI GACNNNGTC 1 cut(s) 1002
PkrI GCNGC 2 cut(s) 788, 1370
PleI GAGTC 2 cut(s) 271, 1395
PpsI GAGTC 2 cut(s) 271, 1395
PscI ACATGT 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 1468
Psp6I CCWGG 2 cut(s) 842, 879
PspFI CCCAGC 1 cut(s) 923
PspGI CCWGG 2 cut(s) 842, 879
PspN4I GGNNCC 1 cut(s) 1542
PspPI GGNCC 2 cut(s) 937, 1590
PstI CTGCAG 1 cut(s) 1357
PsuI RGATCY 1 cut(s) 1501
PsyI GACNNNGTC 1 cut(s) 1002
RseI CAYNNNNRTG 3 cut(s) 1014, 1182, 1551
SacI GAGCTC 1 cut(s) 1468
SaqAI TTAA 4 cut(s) 582, 738, 1472, 1688
SatI GCNGC 2 cut(s) 787, 1369
Sau3AI GATC 8 cut(s) 84, 228, 712, 855, 898, 1248, 1378, 1501
Sau96I GGNCC 2 cut(s) 937, 1590
SchI GAGTC 2 cut(s) 272, 1396
ScrFI CCNGG 3 cut(s) 538, 844, 881
SduI GDGCHC 4 cut(s) 336, 652, 1105, 1468
SfaNI GCATC 5 cut(s) 810, 814, 1033, 1081, 1216
SfcI CTRYAG 1 cut(s) 1353
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 1 cut(s) 937
SmiMI CAYNNNNRTG 3 cut(s) 1014, 1182, 1551
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
SpeI ACTAGT 2 cut(s) 524, 1151
SphI GCATGC 1 cut(s) 1187
Sse9I AATT 8 cut(s) 59, 145, 192, 847, 1110, 1437, 1625, 1682
SsiI CCGC 2 cut(s) 916, 1412
SstI GAGCTC 1 cut(s) 1468
StyD4I CCNGG 3 cut(s) 536, 842, 879
TaaI ACNGT 1 cut(s) 689
TaqI TCGA 7 cut(s) 242, 347, 606, 652, 666, 725, 1390
TasI AATT 8 cut(s) 59, 145, 192, 847, 1110, 1437, 1625, 1682
TfiI GAWTC 2 cut(s) 470, 722
Tru1I TTAA 4 cut(s) 582, 738, 1472, 1688
Tru9I TTAA 4 cut(s) 582, 738, 1472, 1688
TscAI CASTG 4 cut(s) 361, 865, 1105, 1203
TseFI GTSAC 1 cut(s) 958
TseI GCWGC 2 cut(s) 786, 1368
Tsp45I GTSAC 1 cut(s) 958
TspDTI ATGAA 9 cut(s) 17, 353, 371, 587, 998, 1506, 1646, 1683, 1694
TspGWI ACGGA 1 cut(s) 705
TspRI CASTG 4 cut(s) 361, 865, 1105, 1203
Tth111I GACNNNGTC 1 cut(s) 1002
VneI GTGCAC 1 cut(s) 1101
VpaK11BI GGWCC 1 cut(s) 937
XapI RAATTY 2 cut(s) 847, 1110
XceI RCATGY 2 cut(s) 1075, 1187
XmiI GTMKAC 1 cut(s) 1076
Zsp2I ATGCAT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.