MD04G1219000.v1.1

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001773
Physical Location & Seq
Reverse (-)
30103317 .. 30107248
3932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1219000.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGATTATGGAATTGGTTATTGGGATTTTTGGTTATATCTGGTAAAAATGGTGGAGGAGAAGTCTGGGATTGCGAAAGACGTCACTGAATTGATTGGTAAAACGCCGTTGGTGTATCTCAACCGTGTGGTAGATGGCTGTGTAGCGAAGATTGCTGCGAAGCTGGAGATGATGGAACCATGCTCTAGTGTCAAAGATAGGATCGGATATAGTATGATTCAAGATGCCGAGGAGAAAGGTCTCATTACGCCGGGCGAGAGTGTCCTGGTTGAGCCTACAAGTGGCAATACTGGCATAGGATTGGCATTTATGGCAGCTGCTAAGGGTTACAAACTAATAATCACCATGCCTGCTTCAATGAGTCTTGAAAGACGAACCATTCTTCTAGGATTTGGGGCTGAACTTGTACTCACAGATCCAGCCAGAGGCATGAAAGGGGCTGTTCAGAAGGCTGAAGAGATAGTGGCAAAGACACCCAATGCTTATATGCTTCAGCAATTTGAAAACCCTGCGAACCCAAAGGTGCATTATGAAACTACCGGACCAGAAGTCTGGAAAGGCTCTGGTGGTAAAGTTGATGCGTTTGTGTCTGGGATAGGGACAGGAGGTACGATAACAGGTGTTGGGAAGTACCTCAAAGAACAGAATCCGGACATAAAGATCTATGGTGTAGAACCAGTTGAAAGCGCTGTTTTATCAGGAGGGAAACCTGGCCCTCACAAGATTCAAGGAATTGGTGCTGGCTTCATCCCTGGAGTTTTAGATGTCAACGTGATGGATGAAGTTATTCAAGTATCAAGTGAAGTTGCTATAGAAACTGCTAAGCTTCTTGCATTGAAAGAAGGTTTGCTGGTTGGGATATCATCTGGTGCTGCTGCAGCAGCTGCAATCGAAATTGCAAAGAGGCCAGAAAATGCCGGAAAACTCATTGTTGTGGTCTTCCCCAGCTTCGGAGAGCGTTATCTATCCTCTGTGTTGTTTGAATCTGTGAAGCGGGAGGCTGAGAGCATGGTTTTTGAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

342

Amino Acids

36.28

Weight (kDa)

5.2

Isoelectric Point (pI)

27.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 28 - 315 9.1e-74 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 1 cut(s) 994
AclWI GGATC 2 cut(s) 209, 410
AcuI CTGAAG 2 cut(s) 474, 476
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 3 cut(s) 408, 610, 632
AfeI AGCGCT 1 cut(s) 688
AfiI CCNNNNNNNGG 3 cut(s) 281, 425, 950
AgsI TTSAA 9 cut(s) 221, 357, 368, 503, 683, 728, 791, 838, 983
AhdI GACNNNNNGTC 1 cut(s) 548
AjnI CCWGG 3 cut(s) 264, 709, 751
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 5 cut(s) 163, 317, 826, 884, 948
AluI AGCT 5 cut(s) 163, 317, 826, 884, 948
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 2 cut(s) 209, 410
AlwNI CAGNNNCTG 1 cut(s) 884
Aor13HI TCCGGA 1 cut(s) 649
Aor51HI AGCGCT 1 cut(s) 688
AoxI GGCC 2 cut(s) 712, 905
ApeKI GCWGC 8 cut(s) 155, 314, 317, 872, 875, 878, 881, 884
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 689
AspS9I GGNCC 2 cut(s) 542, 713
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 334
AvaII GGWCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 1023
BbsI GAAGAC 1 cut(s) 931
BbvI GCAGC 8 cut(s) 142, 304, 326, 859, 862, 871, 890, 893
BccI CCATC 3 cut(s) 128, 166, 769
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 3 cut(s) 266, 711, 753
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 2 cut(s) 186, 386
BfmI CTRYAG 2 cut(s) 810, 876
BfoI RGCGCY 1 cut(s) 690
BglII AGATCT 1 cut(s) 660
BisI GCNGC 8 cut(s) 156, 315, 318, 873, 876, 879, 882, 885
BlpI GCTNAGC 1 cut(s) 822
BlsI GCNGC 8 cut(s) 157, 316, 319, 874, 877, 880, 883, 886
Bme1390I CCNGG 4 cut(s) 252, 266, 711, 753
Bme18I GGWCC 1 cut(s) 542
BmeRI GACNNNNNGTC 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 542, 713
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 4 cut(s) 252, 266, 711, 753
BmsI GCATC 2 cut(s) 214, 568
BpiI GAAGAC 1 cut(s) 931
BpmI CTGGAG 2 cut(s) 185, 774
Bpu10I CCTNAGC 1 cut(s) 321
Bpu1102I GCTNAGC 1 cut(s) 822
BpuMI CCSGG 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 228, 751
BsaWI WCCGGW 2 cut(s) 539, 649
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 425, 950
Bse1I ACTGG 2 cut(s) 295, 677
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 3 cut(s) 266, 711, 753
BseDI CCNNGG 2 cut(s) 228, 751
BseGI GGATG 2 cut(s) 747, 784
BseLI CCNNNNNNNGG 3 cut(s) 281, 425, 950
BseMII CTCAG 1 cut(s) 993
BseNI ACTGG 2 cut(s) 295, 677
BseRI GAGGAG 2 cut(s) 71, 245
BseXI GCAGC 8 cut(s) 142, 304, 326, 859, 862, 871, 890, 893
BseYI CCCAGC 1 cut(s) 944
BshFI GGCC 2 cut(s) 714, 907
BsiSI CCGG 4 cut(s) 251, 540, 650, 918
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 3 cut(s) 281, 425, 950
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 2 cut(s) 714, 907
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 1023
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 3 cut(s) 201, 415, 660
Bsp1720I GCTNAGC 1 cut(s) 822
BspACI CCGC 1 cut(s) 994
BspANI GGCC 2 cut(s) 714, 907
BspCNI CTCAG 1 cut(s) 994
BspEI TCCGGA 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 177
BspMAI CTGCAG 1 cut(s) 880
BspPI GGATC 2 cut(s) 209, 410
BspTNI GGTCTC 1 cut(s) 245
BsrI ACTGG 2 cut(s) 295, 677
BssECI CCNNGG 2 cut(s) 228, 751
BssMI GATC 3 cut(s) 201, 415, 660
BssNI GRCGYC 1 cut(s) 81
Bst2UI CCWGG 3 cut(s) 266, 711, 753
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 1 cut(s) 81
BstAPI GCANNNNNTGC 1 cut(s) 884
BstC8I GCNNGC 2 cut(s) 351, 742
BstDEI CTNAG 3 cut(s) 321, 822, 1002
BstF5I GGATG 2 cut(s) 747, 784
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 1 cut(s) 689
BstKTI GATC 3 cut(s) 204, 418, 663
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 3 cut(s) 201, 415, 660
BstMWI GCNNNNNNNGC 6 cut(s) 152, 291, 311, 878, 881, 884
BstNI CCWGG 3 cut(s) 266, 711, 753
BstSCI CCNGG 4 cut(s) 250, 264, 709, 751
BstSFI CTRYAG 2 cut(s) 810, 876
BstV1I GCAGC 8 cut(s) 142, 304, 326, 859, 862, 871, 890, 893
BstV2I GAAGAC 1 cut(s) 931
BstX2I RGATCY 2 cut(s) 415, 660
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 2 cut(s) 415, 660
BsuRI GGCC 2 cut(s) 714, 907
BtsCI GGATG 2 cut(s) 747, 784
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 351, 742
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 1 cut(s) 689
Cfr13I GGNCC 2 cut(s) 542, 713
Csp6I GTAC 3 cut(s) 407, 609, 631
CviAII CATG 4 cut(s) 180, 346, 430, 1009
CviQI GTAC 3 cut(s) 407, 609, 631
DdeI CTNAG 3 cut(s) 321, 822, 1002
DpnI GATC 3 cut(s) 203, 417, 662
DpnII GATC 3 cut(s) 201, 415, 660
DriI GACNNNNNGTC 1 cut(s) 548
Eam1104I CTCTTC 1 cut(s) 450
Eam1105I GACNNNNNGTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 861
Eco47I GGWCC 1 cut(s) 542
Eco47III AGCGCT 1 cut(s) 688
Eco57I CTGAAG 2 cut(s) 474, 476
EcoRII CCWGG 3 cut(s) 264, 709, 751
EcoRV GATATC 1 cut(s) 861
EcoT38I GRGCYC 1 cut(s) 1023
FaeI CATG 4 cut(s) 183, 349, 433, 1012
FaqI GGGAC 1 cut(s) 613
FatI CATG 4 cut(s) 179, 345, 429, 1008
FauI CCCGC 1 cut(s) 987
Fnu4HI GCNGC 8 cut(s) 156, 315, 318, 873, 876, 879, 882, 885
FokI GGATG 2 cut(s) 734, 791
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 8 cut(s) 156, 315, 318, 873, 876, 879, 882, 885
FspBI CTAG 2 cut(s) 186, 386
GlaI GCGC 1 cut(s) 688
GluI GCNGC 8 cut(s) 156, 315, 318, 873, 876, 879, 882, 885
GsaI CCCAGC 1 cut(s) 948
GsuI CTGGAG 2 cut(s) 185, 774
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 2 cut(s) 714, 907
HapII CCGG 4 cut(s) 251, 540, 650, 918
HhaI GCGC 1 cut(s) 689
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 183, 349, 433, 1012
Hin6I GCGC 1 cut(s) 687
HinP1I GCGC 1 cut(s) 687
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 5 cut(s) 217, 361, 646, 724, 983
HpaII CCGG 4 cut(s) 251, 540, 650, 918
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 3 cut(s) 206, 447, 953
Hpy188III TCNNGA 5 cut(s) 221, 365, 553, 650, 699
Hpy8I GTNNAC 1 cut(s) 769
HpyAV CCTTC 2 cut(s) 442, 836
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 2 cut(s) 81, 771
HpyCH4V TGCA 5 cut(s) 526, 833, 878, 887, 899
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 291, 311, 878, 881, 884
HpyF3I CTNAG 3 cut(s) 321, 822, 1002
HpySE526I ACGT 2 cut(s) 81, 771
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 183, 349, 433, 1012
HspAI GCGC 1 cut(s) 687
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 3 cut(s) 201, 415, 660
Lsp1109I GCAGC 8 cut(s) 142, 304, 326, 859, 862, 871, 890, 893
LweI GCATC 2 cut(s) 214, 568
MaeI CTAG 2 cut(s) 186, 386
MaeII ACGT 2 cut(s) 81, 771
MaeIII GTNAC 2 cut(s) 82, 326
MalI GATC 3 cut(s) 203, 417, 662
MboI GATC 3 cut(s) 201, 415, 660
MboII GAAGA 4 cut(s) 160, 374, 467, 931
MflI RGATCY 2 cut(s) 415, 660
MhlI GDGCHC 1 cut(s) 1023
MluCI AATT 5 cut(s) 12, 89, 497, 732, 894
MlyI GAGTC 1 cut(s) 370
MroI TCCGGA 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 2 cut(s) 317, 884
MspI CCGG 4 cut(s) 251, 540, 650, 918
MspR9I CCNGG 4 cut(s) 252, 266, 711, 753
MvaI CCWGG 3 cut(s) 266, 711, 753
MwoI GCNNNNNNNGC 6 cut(s) 152, 291, 311, 878, 881, 884
NciI CCSGG 1 cut(s) 252
NdeII GATC 3 cut(s) 201, 415, 660
NlaIII CATG 4 cut(s) 183, 349, 433, 1012
NlaIV GGNNCC 1 cut(s) 177
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 1 cut(s) 82
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 4 cut(s) 217, 646, 724, 983
PkrI GCNGC 8 cut(s) 157, 316, 319, 874, 877, 880, 883, 886
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
Psp6I CCWGG 3 cut(s) 264, 709, 751
PspFI CCCAGC 1 cut(s) 944
PspGI CCWGG 3 cut(s) 264, 709, 751
PspN4I GGNNCC 1 cut(s) 177
PspPI GGNCC 2 cut(s) 542, 713
PstI CTGCAG 1 cut(s) 880
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 2 cut(s) 415, 660
PvuII CAGCTG 2 cut(s) 317, 884
RsaI GTAC 3 cut(s) 408, 610, 632
RsaNI GTAC 3 cut(s) 407, 609, 631
RseI CAYNNNNRTG 1 cut(s) 932
SatI GCNGC 8 cut(s) 156, 315, 318, 873, 876, 879, 882, 885
Sau3AI GATC 3 cut(s) 201, 415, 660
Sau96I GGNCC 2 cut(s) 542, 713
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 4 cut(s) 252, 266, 711, 753
SduI GDGCHC 1 cut(s) 1023
SfaNI GCATC 2 cut(s) 214, 568
SfcI CTRYAG 2 cut(s) 810, 876
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 1 cut(s) 932
Sse9I AATT 5 cut(s) 12, 89, 497, 732, 894
SsiI CCGC 1 cut(s) 994
SspMI CTAG 2 cut(s) 186, 386
StyD4I CCNGG 4 cut(s) 250, 264, 709, 751
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 2 cut(s) 84, 774
TaqI TCGA 1 cut(s) 891
TasI AATT 5 cut(s) 12, 89, 497, 732, 894
TatI WGTACW 1 cut(s) 406
TfiI GAWTC 4 cut(s) 217, 646, 724, 983
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 8 cut(s) 155, 314, 317, 872, 875, 878, 881, 884
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 4 cut(s) 446, 546, 736, 795
TspRI CASTG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 542
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 2 cut(s) 186, 386
ZraI GACGTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.