MD12G1236000.v1.1

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001773
Physical Location & Seq
Reverse (-)
31017931 .. 31021537
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1236000.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGAAATCGTTATTGGGGTTGTTGGTTGTTTAATATATCTGGTAAAAATGGTGGAGGAGAAGTCCGGGATTGCGAAAGACGTCACTGAATTGATCGGTCAAACACCGTTGGTGTATCTCAACCGTGTGGTAGATGGGTGTGTAGCAAAGATTGCTGCAAAGCTGGAGATGATGGAGCCATGCTCTAGTGTCAAAGACAGGATCGGATATAGTATGATTAAAGATGCTGAGGAGAAAGGTCTCATTACGCCTGGCGAGAGTGTCCTGATTGAGCCTACAAGTGGCAATACTGGCATAGGATTGGCGTTCATGGCAGCTGCTAAGGGTTACAAACTAATAATCACCATGCCTGCTTCAATGAGTCTTGAAAGAAGGACCATTCTTCGAGGATTTGGGGCTGAACTTGTCCTCACAGATCCAGCCAGAGGCATGAAAGGGGCTGTTCAAAAGGCTGAAGAGATAGTGGCAAAGACACCCAATGCTTATATGCTTCAGCAATTCGAAAACCCTGCCAACCCAAAGGTGCATTATGAAACTACTGGACCAGAAATCTGGAAAGGCTCTGGTGGTAAAGTTGATGCCTTTGTATCTGGGATAGGGACAGGAGGTACGATAACAGGTGTTGGGAAGTACCTCAAAGAACAAAATCCGGACATTAAGGTTGGCCCTTTCTTTGCACAGTATAATTTGACTTCTTATATCTTAGGCCCTCACAAGATCCAAGGAATTGGTGCTGGCTTCATCCCTGGAGTTTTAGACGTCAACCTGATTGACGAAGTTATTCAAGTAACAAGTGAAGTGGCTATAGAAACTGCTAAGCTTCTTGCATTGAAAGAAGGTTTGCTGGTAGGAATATCATCTGGTGCTGCTGCTGCAGCGGCAATTGAAATTGCTAAGAGGCCTGAAAATGCTGGAAAGCTCATTGTTGTGGTCTTCCCCAGCTTCGGAGAGCGTTATCTATCCTCCGTGTTGTTTGAATCTGTGAAGCGGGAGGCCGAGAGCATGGTTTTTGAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

340

Amino Acids

36.07

Weight (kDa)

5.52

Isoelectric Point (pI)

29.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 28 - 313 6.4e-69 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 84, 762
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 2 cut(s) 878, 988
AclWI GGATC 3 cut(s) 209, 410, 712
AcuI CTGAAG 2 cut(s) 474, 476
AcyI GRCGYC 2 cut(s) 81, 759
AfaI GTAC 2 cut(s) 610, 632
AfiI CCNNNNNNNGG 3 cut(s) 281, 425, 944
AgsI TTSAA 7 cut(s) 357, 368, 446, 785, 832, 887, 977
AjnI CCWGG 2 cut(s) 250, 745
AjuI GAANNNNNNNTTGG 1 cut(s) 29
AluBI AGCT 5 cut(s) 163, 317, 820, 919, 942
AluI AGCT 5 cut(s) 163, 317, 820, 919, 942
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 3 cut(s) 209, 410, 712
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 4 cut(s) 664, 706, 899, 993
ApeKI GCWGC 7 cut(s) 155, 314, 317, 866, 869, 872, 875
Asp700I GAANNNNTTC 1 cut(s) 780
AspS9I GGNCC 4 cut(s) 375, 542, 665, 707
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 501
AvaII GGWCC 2 cut(s) 375, 542
BanII GRGCYC 1 cut(s) 1017
BbsI GAAGAC 1 cut(s) 925
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 7 cut(s) 142, 304, 326, 853, 856, 859, 887
BccI CCATC 2 cut(s) 128, 166
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 2 cut(s) 252, 747
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 186
BfmI CTRYAG 2 cut(s) 804, 873
BisI GCNGC 8 cut(s) 156, 315, 318, 867, 870, 873, 876, 879
BlpI GCTNAGC 1 cut(s) 816
BlsI GCNGC 8 cut(s) 157, 316, 319, 868, 871, 874, 877, 880
Bme1390I CCNGG 3 cut(s) 67, 252, 747
Bme18I GGWCC 2 cut(s) 375, 542
BmgT120I GGNCC 4 cut(s) 375, 542, 665, 707
BmiI GGNNCC 1 cut(s) 177
BmrFI CCNGG 3 cut(s) 67, 252, 747
BmsI GCATC 2 cut(s) 214, 568
BpiI GAAGAC 1 cut(s) 925
BplI GAGNNNNNCTC 2 cut(s) 167, 199
BpmI CTGGAG 2 cut(s) 185, 768
Bpu10I CCTNAGC 2 cut(s) 228, 321
Bpu1102I GCTNAGC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 67
BsaHI GRCGYC 2 cut(s) 81, 759
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 721, 745
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 425, 944
Bse1I ACTGG 2 cut(s) 295, 544
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 2 cut(s) 252, 747
BseDI CCNNGG 2 cut(s) 721, 745
BseGI GGATG 1 cut(s) 741
BseLI CCNNNNNNNGG 3 cut(s) 281, 425, 944
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 2 cut(s) 295, 544
BseRI GAGGAG 2 cut(s) 71, 245
BseXI GCAGC 7 cut(s) 142, 304, 326, 853, 856, 859, 887
BseYI CCCAGC 1 cut(s) 938
BshFI GGCC 4 cut(s) 666, 708, 901, 995
BsiSI CCGG 2 cut(s) 66, 650
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 3 cut(s) 281, 425, 944
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 4 cut(s) 666, 708, 901, 995
Bso31I GGTCTC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 1017
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 4 cut(s) 93, 201, 415, 717
Bsp1720I GCTNAGC 1 cut(s) 816
BspACI CCGC 2 cut(s) 878, 988
BspANI GGCC 4 cut(s) 666, 708, 901, 995
BspCNI CTCAG 1 cut(s) 220
BspEI TCCGGA 1 cut(s) 649
BspLI GGNNCC 1 cut(s) 177
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 3 cut(s) 209, 410, 712
BspT104I TTCGAA 1 cut(s) 501
BspTNI GGTCTC 1 cut(s) 245
BsrI ACTGG 2 cut(s) 295, 544
BssECI CCNNGG 2 cut(s) 721, 745
BssMI GATC 4 cut(s) 93, 201, 415, 717
BssNI GRCGYC 2 cut(s) 81, 759
BssT1I CCWWGG 1 cut(s) 721
Bst2UI CCWGG 2 cut(s) 252, 747
Bst4CI ACNGT 3 cut(s) 108, 125, 681
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 2 cut(s) 81, 759
BstAPI GCANNNNNTGC 1 cut(s) 152
BstBI TTCGAA 1 cut(s) 501
BstC8I GCNNGC 2 cut(s) 351, 736
BstDEI CTNAG 5 cut(s) 228, 321, 703, 816, 894
BstF5I GGATG 1 cut(s) 741
BstKTI GATC 4 cut(s) 96, 204, 418, 720
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 4 cut(s) 93, 201, 415, 717
BstMWI GCNNNNNNNGC 6 cut(s) 152, 291, 311, 872, 875, 878
BstNI CCWGG 2 cut(s) 252, 747
BstSCI CCNGG 3 cut(s) 65, 250, 745
BstSFI CTRYAG 2 cut(s) 804, 873
BstV1I GCAGC 7 cut(s) 142, 304, 326, 853, 856, 859, 887
BstV2I GAAGAC 1 cut(s) 925
BstX2I RGATCY 2 cut(s) 415, 717
BstXI CCANNNNNNTGG 2 cut(s) 552, 728
BstYI RGATCY 2 cut(s) 415, 717
BsuRI GGCC 4 cut(s) 666, 708, 901, 995
BtsCI GGATG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 2 cut(s) 351, 736
Cfr13I GGNCC 4 cut(s) 375, 542, 665, 707
Csp6I GTAC 2 cut(s) 609, 631
CspCI CAANNNNNGTGG 2 cut(s) 780, 815
CviAII CATG 5 cut(s) 180, 310, 346, 430, 1003
CviQI GTAC 2 cut(s) 609, 631
DdeI CTNAG 5 cut(s) 228, 321, 703, 816, 894
DpnI GATC 4 cut(s) 95, 203, 417, 719
DpnII GATC 4 cut(s) 93, 201, 415, 717
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 1 cut(s) 721
Eco147I AGGCCT 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 1017
Eco31I GGTCTC 1 cut(s) 245
Eco47I GGWCC 2 cut(s) 375, 542
Eco57I CTGAAG 2 cut(s) 474, 476
EcoO109I RGGNCCY 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 250, 745
EcoT14I CCWWGG 1 cut(s) 721
EcoT38I GRGCYC 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 721
FaeI CATG 5 cut(s) 183, 313, 349, 433, 1006
FaqI GGGAC 1 cut(s) 613
FatI CATG 5 cut(s) 179, 309, 345, 429, 1002
FauI CCCGC 1 cut(s) 981
Fnu4HI GCNGC 8 cut(s) 156, 315, 318, 867, 870, 873, 876, 879
FokI GGATG 1 cut(s) 728
FriOI GRGCYC 1 cut(s) 1017
Fsp4HI GCNGC 8 cut(s) 156, 315, 318, 867, 870, 873, 876, 879
FspBI CTAG 1 cut(s) 186
GluI GCNGC 8 cut(s) 156, 315, 318, 867, 870, 873, 876, 879
GsaI CCCAGC 1 cut(s) 942
GsuI CTGGAG 2 cut(s) 185, 768
HaeIII GGCC 4 cut(s) 666, 708, 901, 995
HapII CCGG 2 cut(s) 66, 650
Hin1I GRCGYC 2 cut(s) 81, 759
Hin1II CATG 5 cut(s) 183, 313, 349, 433, 1006
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 2 cut(s) 361, 977
HpaII CCGG 2 cut(s) 66, 650
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 2 cut(s) 206, 947
Hpy188III TCNNGA 4 cut(s) 265, 365, 553, 650
Hpy8I GTNNAC 1 cut(s) 763
HpyAV CCTTC 2 cut(s) 366, 830
HpyCH4III ACNGT 3 cut(s) 108, 125, 681
HpyCH4IV ACGT 2 cut(s) 81, 759
HpyCH4V TGCA 5 cut(s) 158, 526, 677, 827, 875
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 291, 311, 872, 875, 878
HpyF3I CTNAG 5 cut(s) 228, 321, 703, 816, 894
HpySE526I ACGT 2 cut(s) 81, 759
Hsp92I GRCGYC 2 cut(s) 81, 759
Hsp92II CATG 5 cut(s) 183, 313, 349, 433, 1006
Kpn2I TCCGGA 1 cut(s) 649
Kzo9I GATC 4 cut(s) 93, 201, 415, 717
LmnI GCTCC 1 cut(s) 175
Lsp1109I GCAGC 7 cut(s) 142, 304, 326, 853, 856, 859, 887
LweI GCATC 2 cut(s) 214, 568
MaeI CTAG 1 cut(s) 186
MaeII ACGT 2 cut(s) 81, 759
MaeIII GTNAC 3 cut(s) 82, 326, 787
MalI GATC 4 cut(s) 95, 203, 417, 719
MboI GATC 4 cut(s) 93, 201, 415, 717
MboII GAAGA 3 cut(s) 374, 467, 925
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 2 cut(s) 415, 717
MhlI GDGCHC 1 cut(s) 1017
MluCI AATT 6 cut(s) 89, 497, 685, 726, 882, 888
MlyI GAGTC 1 cut(s) 370
MroI TCCGGA 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 780
MseI TTAA 3 cut(s) 32, 219, 657
MslI CAYNNNNRTG 1 cut(s) 926
MspA1I CMGCKG 2 cut(s) 317, 878
MspI CCGG 2 cut(s) 66, 650
MspR9I CCNGG 3 cut(s) 67, 252, 747
MunI CAATTG 1 cut(s) 882
MvaI CCWGG 2 cut(s) 252, 747
MwoI GCNNNNNNNGC 6 cut(s) 152, 291, 311, 872, 875, 878
NciI CCSGG 1 cut(s) 67
NdeII GATC 4 cut(s) 93, 201, 415, 717
NlaIII CATG 5 cut(s) 183, 313, 349, 433, 1006
NlaIV GGNNCC 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 82
NspV TTCGAA 1 cut(s) 501
PceI AGGCCT 1 cut(s) 901
PdmI GAANNNNTTC 1 cut(s) 780
PfeI GAWTC 1 cut(s) 977
PfoI TCCNGGA 1 cut(s) 65
PkrI GCNGC 8 cut(s) 157, 316, 319, 868, 871, 874, 877, 880
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 250, 745
PspFI CCCAGC 1 cut(s) 938
PspGI CCWGG 2 cut(s) 250, 745
PspN4I GGNNCC 1 cut(s) 177
PspPI GGNCC 4 cut(s) 375, 542, 665, 707
PstI CTGCAG 1 cut(s) 877
PsuI RGATCY 2 cut(s) 415, 717
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 2 cut(s) 610, 632
RsaNI GTAC 2 cut(s) 609, 631
RseI CAYNNNNRTG 1 cut(s) 926
SaqAI TTAA 3 cut(s) 32, 219, 657
SatI GCNGC 8 cut(s) 156, 315, 318, 867, 870, 873, 876, 879
Sau3AI GATC 4 cut(s) 93, 201, 415, 717
Sau96I GGNCC 4 cut(s) 375, 542, 665, 707
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 3 cut(s) 67, 252, 747
SduI GDGCHC 1 cut(s) 1017
SfaNI GCATC 2 cut(s) 214, 568
SfcI CTRYAG 2 cut(s) 804, 873
SfuI TTCGAA 1 cut(s) 501
SinI GGWCC 2 cut(s) 375, 542
SmiMI CAYNNNNRTG 1 cut(s) 926
Sse9I AATT 6 cut(s) 89, 497, 685, 726, 882, 888
SseBI AGGCCT 1 cut(s) 901
SsiI CCGC 2 cut(s) 878, 988
SspMI CTAG 1 cut(s) 186
StuI AGGCCT 1 cut(s) 901
StyD4I CCNGG 3 cut(s) 65, 250, 745
StyI CCWWGG 1 cut(s) 721
TaaI ACNGT 3 cut(s) 108, 125, 681
TaiI ACGT 2 cut(s) 84, 762
TaqI TCGA 2 cut(s) 385, 501
TaqII GACCGA 1 cut(s) 86
TasI AATT 6 cut(s) 89, 497, 685, 726, 882, 888
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 1 cut(s) 977
Tru1I TTAA 3 cut(s) 32, 219, 657
Tru9I TTAA 3 cut(s) 32, 219, 657
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 7 cut(s) 155, 314, 317, 866, 869, 872, 875
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 4 cut(s) 298, 446, 546, 730
TspGWI ACGGA 1 cut(s) 955
TspRI CASTG 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 375, 542
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 1 cut(s) 186
ZraI GACGTC 2 cut(s) 82, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.