MD16G1194200.v1.1

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001773
Physical Location & Seq
Forward (+)
17219702 .. 17223852
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1194200.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCAGTCGAGAAGGCTGGCATTGCCAAAGATGTTACCGAATTAATTGGAAAAACCCCGTTGGTGTATCTGAACCAAGTCGTGGAAGGTTGTGTTGCTCGAATCGCTGCCAAATTGGAAATGATGGAGCCCTGCTCCAGTGTAAAAGACAGGATTGGCTATAGTATGATTGCAGATGCTGAGGCGAAAGGCCTTATCACACCTGGGCAGAGTGTCCTCATCGAGCCAACAAGTGGTAATACTGGGATAGGATTAGCCTTCATGGCAGCAGCTAAGCATTACAAACTTATCATTACAATGCCTGCTTCAATGAGTCTTGAAAGAAGAATCATCCTTCGGGCTTTTGGAGCTGAGTTGGTTCTCACGGATCCTGCAAAGGGCATGAAAGGGGCTGTTCAGAAGGCTGAAGAGATATTGGCAAAGACACCTAATGCCTACATTCTTCAGCAGTTTGAAAATCCGGCCAACCCTAAGATACATTATGAAACCACTGGACCAGAGATATGGGAAGGTTCAGGAGGGAAAGTAGATGCTTTTGTTTCTGGGATAGGTACAGGTGGAACTATAACAGGTGCAGGGAAGTTTCTAAAGGAGAAGAATCCTGACATAAAGCTGTATGGTGTGGAACCGGTCGAAAGTCCCGTCCTAACTGGAGGAAAGCCTGGTCCACACAAGATCCAAGGAATAGGAGCTGGTTTTATCCCCGGTGTTTTGGAAGTCAATATCATCGATGAAGTCATTCAAATATCAAGTGATGAAGCAATTGAAACTGCAAAGCTTCTCGCACATAAAGAGGGTTTGCTTGTGGGAATATCATCTGGAGCTGCTGCCGCCGCTGCAATTAAAATTGCAAAGAGACCAGAAAATGCTGGAAAGCTCATTGTTGTGATATTTCCAAGCTTTGGGGAGAGGTATCTCTCCTCTGTGTTGTTTGAGTCGGTGAGACGGGAAGCTGAAAGCATGACTTTCGAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000096 GO:0000097 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005777 GO:0005829 GO:0005886 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0007568 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009507 GO:0009532 GO:0009536 GO:0009566 GO:0009567 GO:0009570 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010035 GO:0010038 GO:0010043 GO:0016020 GO:0016043 GO:0016049 GO:0016053 GO:0016740 GO:0016765 GO:0019344 GO:0019752 GO:0019842 GO:0019953 GO:0022414 GO:0030154 GO:0030170 GO:0031090 GO:0032501 GO:0032502 GO:0032989 GO:0036094 GO:0040007 GO:0042221 GO:0042579 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0046394 GO:0046686 GO:0048037 GO:0048046 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0050662 GO:0050896 GO:0051704 GO:0060560 GO:0070279 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:0098588 GO:0098805 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

326

Amino Acids

34.31

Weight (kDa)

5.94

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 12 - 299 1.3e-75 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 82, 233, 902
AciI CCGC 2 cut(s) 831, 834
AclWI GGATC 3 cut(s) 362, 375, 670
AcoI YGGCCR 1 cut(s) 462
AcuI CTGAAG 2 cut(s) 426, 428
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 4 cut(s) 82, 233, 377, 902
AgeI ACCGGT 1 cut(s) 628
AgsI TTSAA 5 cut(s) 309, 320, 455, 743, 767
AjnI CCWGG 2 cut(s) 202, 661
AluBI AGCT 9 cut(s) 272, 350, 613, 692, 778, 824, 877, 900, 953
AluI AGCT 9 cut(s) 272, 350, 613, 692, 778, 824, 877, 900, 953
Alw26I GTCTC 2 cut(s) 850, 937
AlwI GGATC 3 cut(s) 362, 375, 670
AlwNI CAGNNNCTG 1 cut(s) 179
AoxI GGCC 2 cut(s) 190, 462
ApeKI GCWGC 6 cut(s) 107, 266, 269, 824, 827, 836
ArsI GACNNNNNNTTYG 2 cut(s) 627, 659
AseI ATTAAT 1 cut(s) 44
AsiGI ACCGGT 1 cut(s) 628
Asp700I GAANNNNTTC 1 cut(s) 738
AspS9I GGNCC 2 cut(s) 494, 665
AsuC2I CCSGG 1 cut(s) 705
AsuHPI GGTGA 1 cut(s) 952
AvaII GGWCC 2 cut(s) 494, 665
BamHI GGATCC 1 cut(s) 367
BanII GRGCYC 2 cut(s) 132, 975
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 6 cut(s) 94, 278, 281, 811, 814, 823
BccI CCATC 1 cut(s) 118
BcgI CGANNNNNNTGC 1 cut(s) 949
BciT130I CCWGG 2 cut(s) 204, 663
BcnI CCSGG 1 cut(s) 705
BcoDI GTCTC 2 cut(s) 850, 937
BfmI CTRYAG 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
BlpI GCTNAGC 1 cut(s) 273
BlsI GCNGC 8 cut(s) 109, 268, 271, 826, 829, 832, 835, 838
Bme1390I CCNGG 3 cut(s) 204, 663, 705
Bme18I GGWCC 2 cut(s) 494, 665
BmgT120I GGNCC 2 cut(s) 494, 665
BmiI GGNNCC 3 cut(s) 129, 369, 627
BmrFI CCNGG 3 cut(s) 204, 663, 705
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 166, 520
BmuI ACTGGG 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 119, 151
BpmI CTGGAG 3 cut(s) 121, 672, 840
Bpu10I CCTNAGC 1 cut(s) 180
Bpu1102I GCTNAGC 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 705
Bsa29I ATCGAT 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 850
BsaJI CCNNGG 3 cut(s) 203, 679, 703
BsaWI WCCGGW 1 cut(s) 628
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 233, 377, 902
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 4 cut(s) 138, 247, 496, 655
Bse3DI GCAATG 1 cut(s) 21
BseBI CCWGG 2 cut(s) 204, 663
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 3 cut(s) 203, 679, 703
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 4 cut(s) 82, 233, 377, 902
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 2 cut(s) 171, 342
BseNI ACTGG 4 cut(s) 138, 247, 496, 655
BseRI GAGGAG 1 cut(s) 910
BseXI GCAGC 6 cut(s) 94, 278, 281, 811, 814, 823
BsgI GTGCAG 1 cut(s) 594
Bsh1285I CGRYCG 1 cut(s) 633
BshFI GGCC 2 cut(s) 192, 464
BshTI ACCGGT 1 cut(s) 628
BshVI ATCGAT 1 cut(s) 729
BsiEI CGRYCG 1 cut(s) 633
BsiSI CCGG 3 cut(s) 461, 629, 705
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 4 cut(s) 82, 233, 377, 902
BsmAI GTCTC 2 cut(s) 850, 937
BsmBI CGTCTC 1 cut(s) 937
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 2 cut(s) 192, 464
Bso31I GGTCTC 1 cut(s) 850
Bsp1286I GDGCHC 2 cut(s) 132, 975
Bsp143I GATC 2 cut(s) 367, 675
Bsp1720I GCTNAGC 1 cut(s) 273
BspACI CCGC 2 cut(s) 831, 834
BspANI GGCC 2 cut(s) 192, 464
BspCNI CTCAG 2 cut(s) 172, 343
BspDI ATCGAT 1 cut(s) 729
BspLI GGNNCC 3 cut(s) 129, 369, 627
BspPI GGATC 3 cut(s) 362, 375, 670
BspTNI GGTCTC 1 cut(s) 850
BsrDI GCAATG 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 4 cut(s) 138, 247, 496, 655
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 3 cut(s) 203, 679, 703
BssMI GATC 2 cut(s) 367, 675
BssT1I CCWWGG 1 cut(s) 679
Bst2UI CCWGG 2 cut(s) 204, 663
Bst6I CTCTTC 1 cut(s) 402
BstC8I GCNNGC 2 cut(s) 19, 303
BstDEI CTNAG 4 cut(s) 180, 273, 351, 471
BstF5I GGATG 1 cut(s) 330
BstKTI GATC 2 cut(s) 370, 678
BstMAI GTCTC 2 cut(s) 850, 937
BstMBI GATC 2 cut(s) 367, 675
BstMCI CGRYCG 1 cut(s) 633
BstMWI GCNNNNNNNGC 7 cut(s) 23, 104, 263, 347, 830, 833, 836
BstNI CCWGG 2 cut(s) 204, 663
BstSCI CCNGG 3 cut(s) 202, 661, 703
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 6 cut(s) 94, 278, 281, 811, 814, 823
BstX2I RGATCY 2 cut(s) 367, 675
BstXI CCANNNNNNTGG 1 cut(s) 504
BstYI RGATCY 2 cut(s) 367, 675
Bsu15I ATCGAT 1 cut(s) 729
BsuRI GGCC 2 cut(s) 192, 464
BsuTUI ATCGAT 1 cut(s) 729
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 145, 489
Cac8I GCNNGC 2 cut(s) 19, 303
CaiI CAGNNNCTG 1 cut(s) 179
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 494, 665
ClaI ATCGAT 1 cut(s) 729
Csp6I GTAC 1 cut(s) 552
CspAI ACCGGT 1 cut(s) 628
CviAII CATG 3 cut(s) 262, 382, 961
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 4 cut(s) 180, 273, 351, 471
DpnI GATC 2 cut(s) 369, 677
DpnII GATC 2 cut(s) 367, 675
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 679
Eco147I AGGCCT 1 cut(s) 192
Eco24I GRGCYC 2 cut(s) 132, 975
Eco31I GGTCTC 1 cut(s) 850
Eco47I GGWCC 2 cut(s) 494, 665
Eco57I CTGAAG 2 cut(s) 426, 428
EcoRII CCWGG 2 cut(s) 202, 661
EcoT14I CCWWGG 1 cut(s) 679
EcoT38I GRGCYC 2 cut(s) 132, 975
ErhI CCWWGG 1 cut(s) 679
Esp3I CGTCTC 1 cut(s) 937
FaeI CATG 3 cut(s) 265, 385, 964
FalI AAGNNNNNCTT 1 cut(s) 949
FaqI GGGAC 1 cut(s) 624
FatI CATG 3 cut(s) 261, 381, 960
Fnu4HI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 2 cut(s) 132, 975
Fsp4HI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
GluI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
GsuI CTGGAG 3 cut(s) 121, 672, 840
HaeIII GGCC 2 cut(s) 192, 464
HapII CCGG 3 cut(s) 461, 629, 705
Hin1II CATG 3 cut(s) 265, 385, 964
HindIII AAGCTT 2 cut(s) 776, 898
HinfI GANTC 5 cut(s) 102, 313, 327, 598, 935
HpaII CCGG 3 cut(s) 461, 629, 705
HphI GGTGA 1 cut(s) 952
Hpy166II GTNNAC 1 cut(s) 668
Hpy188I TCNGA 2 cut(s) 72, 399
Hpy188III TCNNGA 5 cut(s) 10, 317, 516, 602, 819
Hpy8I GTNNAC 1 cut(s) 668
HpyAV CCTTC 6 cut(s) 7, 80, 268, 344, 394, 503
HpyCH4V TGCA 6 cut(s) 173, 374, 575, 773, 839, 851
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 104, 263, 347, 830, 833, 836
HpyF3I CTNAG 4 cut(s) 180, 273, 351, 471
Hsp92II CATG 3 cut(s) 265, 385, 964
Kzo9I GATC 2 cut(s) 367, 675
LmnI GCTCC 5 cut(s) 127, 140, 347, 689, 821
Lsp1109I GCAGC 6 cut(s) 94, 278, 281, 811, 814, 823
LweI GCATC 2 cut(s) 166, 520
MaeIII GTNAC 1 cut(s) 34
MalI GATC 2 cut(s) 369, 677
MboI GATC 2 cut(s) 367, 675
MboII GAAGA 4 cut(s) 336, 419, 434, 607
MfeI CAATTG 1 cut(s) 762
MflI RGATCY 2 cut(s) 367, 675
MhlI GDGCHC 2 cut(s) 132, 975
MluCI AATT 6 cut(s) 41, 45, 113, 762, 840, 846
MlyI GAGTC 2 cut(s) 322, 944
MnlI CCTC 7 cut(s) 175, 227, 512, 647, 787, 903, 931
MroXI GAANNNNTTC 1 cut(s) 738
MseI TTAA 2 cut(s) 44, 843
MslI CAYNNNNRTG 2 cut(s) 296, 884
MspA1I CMGCKG 1 cut(s) 836
MspI CCGG 3 cut(s) 461, 629, 705
MspR9I CCNGG 3 cut(s) 204, 663, 705
MunI CAATTG 1 cut(s) 762
MvaI CCWGG 2 cut(s) 204, 663
MwoI GCNNNNNNNGC 7 cut(s) 23, 104, 263, 347, 830, 833, 836
NciI CCSGG 1 cut(s) 705
NdeII GATC 2 cut(s) 367, 675
NlaIII CATG 3 cut(s) 265, 385, 964
NlaIV GGNNCC 3 cut(s) 129, 369, 627
PceI AGGCCT 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 639
PdmI GAANNNNTTC 1 cut(s) 738
PfeI GAWTC 3 cut(s) 102, 327, 598
PflMI CCANNNNNTGG 3 cut(s) 82, 233, 902
PinAI ACCGGT 1 cut(s) 628
PkrI GCNGC 8 cut(s) 109, 268, 271, 826, 829, 832, 835, 838
PleI GAGTC 2 cut(s) 321, 943
PpsI GAGTC 2 cut(s) 321, 943
PshBI ATTAAT 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 202, 661
PspGI CCWGG 2 cut(s) 202, 661
PspN4I GGNNCC 3 cut(s) 129, 369, 627
PspPI GGNCC 2 cut(s) 494, 665
PstNI CAGNNNCTG 1 cut(s) 179
PsuI RGATCY 2 cut(s) 367, 675
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 2 cut(s) 296, 884
SaqAI TTAA 2 cut(s) 44, 843
SatI GCNGC 8 cut(s) 108, 267, 270, 825, 828, 831, 834, 837
Sau3AI GATC 2 cut(s) 367, 675
Sau96I GGNCC 2 cut(s) 494, 665
SchI GAGTC 2 cut(s) 322, 944
ScrFI CCNGG 3 cut(s) 204, 663, 705
SduI GDGCHC 2 cut(s) 132, 975
SfaNI GCATC 2 cut(s) 166, 520
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 2 cut(s) 494, 665
SmiMI CAYNNNNRTG 2 cut(s) 296, 884
Sse9I AATT 6 cut(s) 41, 45, 113, 762, 840, 846
SseBI AGGCCT 1 cut(s) 192
SsiI CCGC 2 cut(s) 831, 834
StuI AGGCCT 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 202, 661, 703
StyI CCWWGG 1 cut(s) 679
TaqI TCGA 6 cut(s) 9, 100, 222, 633, 729, 969
TasI AATT 6 cut(s) 41, 45, 113, 762, 840, 846
TauI GCSGC 2 cut(s) 833, 836
TfiI GAWTC 3 cut(s) 102, 327, 598
Tru1I TTAA 2 cut(s) 44, 843
Tru9I TTAA 2 cut(s) 44, 843
TscAI CASTG 2 cut(s) 145, 496
TseI GCWGC 6 cut(s) 107, 266, 269, 824, 827, 836
TspDTI ATGAA 5 cut(s) 250, 398, 498, 747, 771
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 2 cut(s) 145, 496
Van91I CCANNNNNTGG 3 cut(s) 82, 233, 902
VpaK11BI GGWCC 2 cut(s) 494, 665
VspI ATTAAT 1 cut(s) 44
XmnI GAANNNNTTC 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.