Rroxscaffold_6G00429370

Belongs to the cysteine synthase cystathionine beta- synthase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001773
Physical Location & Seq
Forward (+)
49662027 .. 49666565
4539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00429370.1

Sequence Viewer

Length: 1227 bp
ATGATGACGATAATATTCGCATATATCAAAAAACAGTCACAGCCAGCAAAAATTTCCTTTAAATATCCGGAAGTCTCTCACTTTTGTCATTCGCTTCTTTCTTCTTGTGGGTCAGCTTTTGGTTTCAACTTTCAAGCAGGTAGAAACTTCAAAAAGCCTCACTCTTTTCTCTCATTTCAATTCAAGCATACATTTCTTCTTGAACCCATTTGCATTCTCCCAATAGTCCCACAAAGTTTTCTGGTAAAGATGGTGGAAGAGAAGGCTTGGATTGCAAAAGATGTTACTGAATTGATTGGCAACACTCCATTGGTGTATCTCAACCATGTCGTAGATGGCTGTGTGGCACGGGTTGCTGCTAAGCTGGAGATGATGGAGCCTTGCTCTAGCGTCAAGGATAGGATTGGATACAGTATGATCACAGATGCTGAGGAGAAGGGTCTTATTAAGGCTGGTGAGAGTGTCCTGGTCGAGCCTACTAGTGGCAATACTGGCATAGGATTGGCGTTTATGGCCGCTGCTAAGGGTTACAAACTAATAATCACCATGCCTGCTTCAATGAGTCTTGAAAGACGAACCATTCTTCGAGGTTTTGGAGCTGAGCTTGTTCTCACAGATCCCGCCAAAGGCATGAAGGGGGCTGTTCAGAAGGCACAAGAGATAGTGGACAAGACACCCAATGCTTATATGCTTCAGCAATTTGAGAACCCTGCAAACCCAAAGGTACATTATGAAACTACTGGACCAGAGATCTGGAGAGGCTCTGCTGGTAAAGTTGACGCCTTTGTTTCTGGGATAGGGACTGGAGGTACAATAACAGGTGCTGGGAAGTACCTCAAAGAGCAAAATCCTGATGTTAAGCTCTACGGTGTAGAACCAGTTGAAAGTGCTGTCTTGTCTGGAGGGCGACCTGGCCCTCACAAGATTCAAGGAATTGGTGCTGGCTTCATCCCTGGAGTTTTAGATGTCAGCCTAATTGATGAAGTTATCCAAATATCAAGTGAAGAAGCAATTGAAACAGCCAAGCGTCTAGCATTGAAGGAAGGTTTGTTGGTAGGGATATCATCTGGTGCTGCAGCTGCAGCTGCTATTGAGATTGCAAGGAGGCCAGCAAACGCTGGAAAGCTCATTGTTGTGGTCTTCCCCAGCTTTGGAGAGCGTTACCTCTCCTCTGTATTGTTTGAATCCGTGAAGCGGGAGGCAGAGAACATGGTTTTTGAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

408

Amino Acids

43.96

Weight (kDa)

8.13

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 95 - 382 3.3e-74 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AccIII TCCGGA 1 cut(s) 67
AciI CCGC 3 cut(s) 516, 621, 1195
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 1 cut(s) 513
AcsI RAATTY 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 677
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 3 cut(s) 726, 811, 833
AfiI CCNNNNNNNGG 4 cut(s) 482, 626, 1151, 1194
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 3 cut(s) 465, 910, 952
AluBI AGCT 9 cut(s) 116, 364, 599, 604, 862, 1079, 1085, 1126, 1149
AluI AGCT 9 cut(s) 116, 364, 599, 604, 862, 1079, 1085, 1126, 1149
Alw26I GTCTC 1 cut(s) 79
AlwI GGATC 1 cut(s) 611
AlwNI CAGNNNCTG 2 cut(s) 428, 824
Aor13HI TCCGGA 1 cut(s) 67
AoxI GGCC 3 cut(s) 513, 913, 1106
ApeKI GCWGC 7 cut(s) 356, 518, 1073, 1076, 1079, 1082, 1085
ApoI RAATTY 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 743, 914
AsuHPI GGTGA 2 cut(s) 467, 535
AvaII GGWCC 1 cut(s) 743
BanII GRGCYC 1 cut(s) 1224
BbsI GAAGAC 1 cut(s) 1132
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 7 cut(s) 343, 505, 1060, 1066, 1072, 1088, 1094
BccI CCATC 3 cut(s) 244, 329, 367
BciT130I CCWGG 3 cut(s) 467, 912, 954
BciVI GTATCC 1 cut(s) 401
BclI TGATCA 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 79
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 3 cut(s) 387, 480, 1031
BfmI CTRYAG 2 cut(s) 1074, 1080
BfuAI ACCTGC 1 cut(s) 128
BfuI GTATCC 1 cut(s) 401
BglII AGATCT 1 cut(s) 750
BisI GCNGC 8 cut(s) 357, 516, 519, 1074, 1077, 1080, 1083, 1086
BlpI GCTNAGC 2 cut(s) 360, 600
BlsI GCNGC 8 cut(s) 358, 517, 520, 1075, 1078, 1081, 1084, 1087
Bme1390I CCNGG 3 cut(s) 467, 912, 954
Bme18I GGWCC 1 cut(s) 743
BmgT120I GGNCC 2 cut(s) 743, 914
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 3 cut(s) 467, 912, 954
BmsI GCATC 1 cut(s) 415
BpiI GAAGAC 1 cut(s) 1132
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpmI CTGGAG 5 cut(s) 386, 775, 825, 921, 975
Bpu10I CCTNAGC 2 cut(s) 429, 522
Bpu1102I GCTNAGC 2 cut(s) 360, 600
BsaHI GRCGYC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 952
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 4 cut(s) 482, 626, 1151, 1194
Bse1I ACTGG 4 cut(s) 496, 745, 808, 878
BseAI TCCGGA 1 cut(s) 67
BseBI CCWGG 3 cut(s) 467, 912, 954
BseDI CCNNGG 1 cut(s) 952
BseGI GGATG 1 cut(s) 948
BseLI CCNNNNNNNGG 4 cut(s) 482, 626, 1151, 1194
BseMII CTCAG 2 cut(s) 420, 591
BseNI ACTGG 4 cut(s) 496, 745, 808, 878
BseRI GAGGAG 2 cut(s) 446, 1159
BseXI GCAGC 7 cut(s) 343, 505, 1060, 1066, 1072, 1088, 1094
BseYI CCCAGC 2 cut(s) 824, 1145
BshFI GGCC 3 cut(s) 515, 915, 1108
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 2 cut(s) 212, 814
BslI CCNNNNNNNGG 4 cut(s) 482, 626, 1151, 1194
BsmAI GTCTC 1 cut(s) 79
BsmFI GGGAC 2 cut(s) 212, 814
BsmI GAATGC 1 cut(s) 213
BsnI GGCC 3 cut(s) 515, 915, 1108
Bsp1286I GDGCHC 1 cut(s) 1224
Bsp13I TCCGGA 1 cut(s) 67
Bsp143I GATC 3 cut(s) 417, 616, 750
Bsp1720I GCTNAGC 2 cut(s) 360, 600
BspACI CCGC 3 cut(s) 516, 621, 1195
BspANI GGCC 3 cut(s) 515, 915, 1108
BspCNI CTCAG 2 cut(s) 421, 592
BspEI TCCGGA 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 378
BspMAI CTGCAG 2 cut(s) 1078, 1084
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 1 cut(s) 611
BsrI ACTGG 4 cut(s) 496, 745, 808, 878
BssECI CCNNGG 1 cut(s) 952
BssMI GATC 3 cut(s) 417, 616, 750
BssNI GRCGYC 1 cut(s) 780
Bst2UI CCWGG 3 cut(s) 467, 912, 954
Bst4CI ACNGT 3 cut(s) 36, 413, 869
Bst6I CTCTTC 1 cut(s) 252
BstACI GRCGYC 1 cut(s) 780
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 4 cut(s) 45, 552, 943, 1110
BstDEI CTNAG 4 cut(s) 360, 429, 522, 600
BstF5I GGATG 1 cut(s) 948
BstKTI GATC 3 cut(s) 420, 619, 753
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 3 cut(s) 417, 616, 750
BstMWI GCNNNNNNNGC 7 cut(s) 272, 353, 492, 512, 1079, 1082, 1085
BstNI CCWGG 3 cut(s) 467, 912, 954
BstSCI CCNGG 3 cut(s) 465, 910, 952
BstSFI CTRYAG 2 cut(s) 1074, 1080
BstV1I GCAGC 7 cut(s) 343, 505, 1060, 1066, 1072, 1088, 1094
BstV2I GAAGAC 1 cut(s) 1132
BstX2I RGATCY 2 cut(s) 616, 750
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 2 cut(s) 616, 750
BsuI GTATCC 1 cut(s) 401
BsuRI GGCC 3 cut(s) 515, 915, 1108
BtsCI GGATG 1 cut(s) 948
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 4 cut(s) 45, 552, 943, 1110
CaiI CAGNNNCTG 2 cut(s) 428, 824
Cfr13I GGNCC 2 cut(s) 743, 914
CseI GACGC 3 cut(s) 379, 788, 1016
Csp6I GTAC 3 cut(s) 725, 810, 832
CviAII CATG 4 cut(s) 326, 547, 631, 1210
CviQI GTAC 3 cut(s) 725, 810, 832
DdeI CTNAG 4 cut(s) 360, 429, 522, 600
DpnI GATC 3 cut(s) 419, 618, 752
DpnII GATC 3 cut(s) 417, 616, 750
DraI TTTAAA 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 513
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 1224
Eco32I GATATC 1 cut(s) 1062
Eco47I GGWCC 1 cut(s) 743
Eco57I CTGAAG 1 cut(s) 677
EcoRII CCWGG 3 cut(s) 465, 910, 952
EcoRV GATATC 1 cut(s) 1062
EcoT38I GRGCYC 1 cut(s) 1224
FaeI CATG 4 cut(s) 329, 550, 634, 1213
FaqI GGGAC 2 cut(s) 212, 814
FatI CATG 4 cut(s) 325, 546, 630, 1209
FauI CCCGC 2 cut(s) 628, 1188
FbaI TGATCA 1 cut(s) 417
Fnu4HI GCNGC 8 cut(s) 357, 516, 519, 1074, 1077, 1080, 1083, 1086
FokI GGATG 1 cut(s) 935
FriOI GRGCYC 1 cut(s) 1224
Fsp4HI GCNGC 8 cut(s) 357, 516, 519, 1074, 1077, 1080, 1083, 1086
FspBI CTAG 3 cut(s) 387, 480, 1031
GluI GCNGC 8 cut(s) 357, 516, 519, 1074, 1077, 1080, 1083, 1086
GsaI CCCAGC 2 cut(s) 828, 1149
GsuI CTGGAG 5 cut(s) 386, 775, 825, 921, 975
HaeIII GGCC 3 cut(s) 515, 915, 1108
HapII CCGG 1 cut(s) 68
HgaI GACGC 3 cut(s) 379, 788, 1016
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 4 cut(s) 329, 550, 634, 1213
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HinfI GANTC 3 cut(s) 562, 925, 1184
HpaII CCGG 1 cut(s) 68
HphI GGTGA 2 cut(s) 467, 535
Hpy166II GTNNAC 2 cut(s) 667, 778
Hpy188I TCNGA 1 cut(s) 648
Hpy188III TCNNGA 6 cut(s) 68, 200, 566, 754, 851, 900
Hpy8I GTNNAC 2 cut(s) 667, 778
HpyAV CCTTC 6 cut(s) 256, 430, 628, 643, 1033, 1037
HpyCH4III ACNGT 3 cut(s) 36, 413, 869
HpyCH4V TGCA 6 cut(s) 213, 275, 713, 1076, 1082, 1100
HpyF10VI GCNNNNNNNGC 7 cut(s) 272, 353, 492, 512, 1079, 1082, 1085
HpyF3I CTNAG 4 cut(s) 360, 429, 522, 600
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 4 cut(s) 329, 550, 634, 1213
Kpn2I TCCGGA 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 3 cut(s) 417, 616, 750
LmnI GCTCC 2 cut(s) 376, 596
Lsp1109I GCAGC 7 cut(s) 343, 505, 1060, 1066, 1072, 1088, 1094
LweI GCATC 1 cut(s) 415
MaeI CTAG 3 cut(s) 387, 480, 1031
MaeIII GTNAC 4 cut(s) 36, 283, 527, 1160
MalI GATC 3 cut(s) 419, 618, 752
MboI GATC 3 cut(s) 417, 616, 750
MboII GAAGA 6 cut(s) 93, 188, 269, 575, 1016, 1132
MfeI CAATTG 1 cut(s) 1011
MflI RGATCY 2 cut(s) 616, 750
MhlI GDGCHC 1 cut(s) 1224
MluCI AATT 7 cut(s) 51, 179, 290, 698, 933, 975, 1011
MlyI GAGTC 1 cut(s) 571
MroI TCCGGA 1 cut(s) 67
MseI TTAA 3 cut(s) 60, 447, 858
MslI CAYNNNNRTG 1 cut(s) 1133
MspA1I CMGCKG 3 cut(s) 518, 1079, 1085
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 3 cut(s) 467, 912, 954
MunI CAATTG 1 cut(s) 1011
Mva1269I GAATGC 1 cut(s) 213
MvaI CCWGG 3 cut(s) 467, 912, 954
MwoI GCNNNNNNNGC 7 cut(s) 272, 353, 492, 512, 1079, 1082, 1085
NdeII GATC 3 cut(s) 417, 616, 750
NlaIII CATG 4 cut(s) 329, 550, 634, 1213
NlaIV GGNNCC 1 cut(s) 378
NmuCI GTSAC 1 cut(s) 36
PctI GAATGC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 925, 1184
PkrI GCNGC 8 cut(s) 358, 517, 520, 1075, 1078, 1081, 1084, 1087
PleI GAGTC 1 cut(s) 570
PpsI GAGTC 1 cut(s) 570
Psp6I CCWGG 3 cut(s) 465, 910, 952
PspFI CCCAGC 2 cut(s) 824, 1145
PspGI CCWGG 3 cut(s) 465, 910, 952
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 2 cut(s) 743, 914
PstI CTGCAG 2 cut(s) 1078, 1084
PstNI CAGNNNCTG 2 cut(s) 428, 824
PsuI RGATCY 2 cut(s) 616, 750
PvuII CAGCTG 2 cut(s) 1079, 1085
RsaI GTAC 3 cut(s) 726, 811, 833
RsaNI GTAC 3 cut(s) 725, 810, 832
RseI CAYNNNNRTG 1 cut(s) 1133
SaqAI TTAA 3 cut(s) 60, 447, 858
SatI GCNGC 8 cut(s) 357, 516, 519, 1074, 1077, 1080, 1083, 1086
Sau3AI GATC 3 cut(s) 417, 616, 750
Sau96I GGNCC 2 cut(s) 743, 914
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 3 cut(s) 467, 912, 954
SduI GDGCHC 1 cut(s) 1224
SfaNI GCATC 1 cut(s) 415
SfcI CTRYAG 2 cut(s) 1074, 1080
SinI GGWCC 1 cut(s) 743
SmiMI CAYNNNNRTG 1 cut(s) 1133
SpeI ACTAGT 1 cut(s) 479
Sse9I AATT 7 cut(s) 51, 179, 290, 698, 933, 975, 1011
SsiI CCGC 3 cut(s) 516, 621, 1195
SspI AATATT 1 cut(s) 15
SspMI CTAG 3 cut(s) 387, 480, 1031
StyD4I CCNGG 3 cut(s) 465, 910, 952
TaaI ACNGT 3 cut(s) 36, 413, 869
TaqI TCGA 2 cut(s) 471, 586
TasI AATT 7 cut(s) 51, 179, 290, 698, 933, 975, 1011
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 925, 1184
Tru1I TTAA 3 cut(s) 60, 447, 858
Tru9I TTAA 3 cut(s) 60, 447, 858
TseFI GTSAC 1 cut(s) 36
TseI GCWGC 7 cut(s) 356, 518, 1073, 1076, 1079, 1082, 1085
Tsp45I GTSAC 1 cut(s) 36
TspDTI ATGAA 4 cut(s) 647, 747, 937, 996
TspGWI ACGGA 1 cut(s) 1177
VpaK11BI GGWCC 1 cut(s) 743
XapI RAATTY 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 3 cut(s) 387, 480, 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.