Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004233

Physical Location & Seq

Genomic Coordinates Forward (+)

19832059 .. 19837715

Gene Structure 5657 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1093500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1965 bp

ATGGAGATGAAGAGAGGAGAGGTTGCGCTTCAATGGTTTAATACTACAGGCCCGAACAACGACCATCTAGTCCTCTCCCCTTACATCTTCCCTGAAAACCACCTTCGTCGCGATTCTCCTTCTGCTTCCTTGCCGGACATCTGTTGGGACCCTAGTCTGGGGAACGTTTCTTATCAAAACGCCATGCTCCCAAATCATCTATACCAAAACCCTAACCCCTCACAAAACACCAATCTCAATAATAATACTGATTTTGCTGATCAGAGCCAAAATCAGCATCATCTCCCTGCTGCTTTGAATCGACTGAGCATATCGAATAACAATTCTCATCATCATGATCATCAGCCGCAGCCTGGTGGTACTGCGTCGAACCTAAGAGCAAACAGCTTCTCAGGAGGTAATGGTTTTACTACTCAATACCCATCCCCTCATCATGTGCAAGAGGAGGAGGAATATTTGGAGAGGTTGAAGAATCAGTATGTTTTGTTGATGGCACAGATGGATCAGTACGTGAGGCTTCATGGATCCTTTTCCAATCATGGTACCACTAATAACCATAATTTCAATGGTGCTCTTCCCCATTCTTTGTCAGCGGCACGCGGTAATTTTCCTTTGATAGAGGTGGAACCTATTTATACAGGAGGAGCGAGTCGGGGTGGGAGTAGTAGTACTTCTAGGCCAAATAATATTGCTCAATTTTCCTCTGCTGATCAATTTGGTGCTTATGGATATCATCCAATGAGGCCTAATAATTCGCTGCGAAACGGATTAACTAGCAGCCGCGGATCATCATCAAGGTATGCTAATGGGTCAGGACGACAGCGTCTACGTACTAATTTCTCATCATCATCGTCATTGGAACAAAGCGGCCAAGTGATGACTAGTAGTAATTTAATTACCAAAGATCATGATCATGGCATCAGGGGATCAAGGTTTGCTAATGGCTCTGCACGGCAGCAGCATCCAAGTACTTTCTCTCTGACTTTAGAAGAAATAAGGGGCCAAATTGCTTTGGTGGCCAAAGATCATGCCGGGTCTAAATTTTTGCAGAAGAAGATGGAGGAGGGCAAGCCGGAGGAAATTACTATCATTTTCTCCGAGGTCATGGAGGCGGATTTCCGTGAATTGATGGTTGATCAATTCGGCAACGAACTTGTTCAGAAACTTGTTGAAGCAGCCAACAAAGAAGGATGGTCCAAAATGCTTGACTTGGTGATGAGTGATGAGAAAAAACTCAAGGACATATGCACTGATCAGCACGGAAGTCGAGCAATGCAGAAACTGCTGGAGCGCGTTGAAACCCGTGAGCAACAGTCGGCATTCATGCGGCTTCTGAAGCATATAACGATTCCCTTGAGCAAGACCCAGCCTGGTTATTATGTGATTCAAGTTTGCTTGAAACATTTCTCTCCTGAATCCACCAAGGGTCTTCTGGAATTAGTATACGAAAACTGCCTTGCTCTTGCAAAAGACAAATTTGGGTGTTGCTTAGTGCAAAGTAGTGTTCATTATGCTTATACAGAAGCTAAAGAGCGTCTTGTGGCTGACATAACTGAAAATGCTCGAGTTCTCTCTGAAGATCCATACGGGAACTATGTCGTGCAGTACATAATTGGATTAAAGAACCCGCGTGTTACAACTGACATACTAGCACAACTTGGAGGTAGTTTTGTTAATCTATGCATGAATAAGTATGGCAGTAATGTGGTTGAGAAGATCTTGAAAGAGGCTGGAGAAGAGCATGTCAATACCATTGTTTACGAGATGATTAATAGTCCACACTTTTTGGCTCTTCTTCAAGATCCTTATGGGAATTATGTTGCTCAATCTGCTTTGGGAGTATCCAAGGGAGCTGCTCATAATACTCTTGTGCAACTCGTTCGGAGCCATTACCCTTATCTCCACAGTCATCTTTACGGGAAAAGGGTGCTGGACAAAACCAGGGGTCACAGGCATCGTTACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUF

Protein Analysis

655

Amino Acids

73.23

Weight (kDa)

7.45

Isoelectric Point (pI)

40.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	484 - 517	4.8e-06	Pumilio-family RNA binding repeat
PUF	PF00806	557 - 590	9.4e-08	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004233)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g30780 FvH4_5g30780
malus_domestica	MD05G1093500.v1.1
prunus_persica	Prupe.8G138200_v2.0.a1 Prupe.8G138200_v2.0.a1
pyrus_communis	pycom15g06920
rosa_chinensis	RchiOBHm_Chr6g0254321 RchiOBHm_Chr6g0254351
rosa_laevigata	RLG00000014133 RLG00000014918 RLG00000014919 RLG00000014923 RLG00000014926
rosa_multiflora	Rmu_sc0006565.1_g000002 Rmu_sc0007138.1_g000009 Rmu_sc0027609.1_g000002
rosa_roxburghii	Rroxscaffold_7G00210870 Rroxscaffold_7G00210910 Rroxscaffold_7G00210930
rosa_rugosa	Rorug05G0552000
rosa_samantha	Rh6AG068500 Rh6AG068800 Rh6AG068900 Rh6BG061200 Rh6CG061000 Rh6CG061100 Rh6CG061500 Rh6DG058000 Rh6DG058300 Rh6DG058400 Rh6DG058700
rosa_wichuraiana	Rw6G006000 Rw6G006040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	68, 822
Acc65I	GGTACC	1 cut(s)	542
AccB1I	GGYRCC	1 cut(s)	542
AccI	GTMKAC	2 cut(s)	826, 1443
AccII	CGCG	5 cut(s)	111, 600, 783, 1293, 1630
AciI	CCGC	9 cut(s)	347, 593, 600, 781, 783, 867, 1112, 1327, 1628
AclI	AACGTT	1 cut(s)	165
AclWI	GGATC	7 cut(s)	510, 519, 532, 793, 934, 1574, 1796
AcoI	YGGCCR	2 cut(s)	868, 1017
AcsI	RAATTY	2 cut(s)	1040, 1475
AcuI	CTGAAG	2 cut(s)	1355, 1596
AfaI	GTAC	7 cut(s)	361, 509, 544, 670, 832, 970, 1607
AfiI	CCNNNNNNNGG	3 cut(s)	157, 158, 353
AhlI	ACTAGT	1 cut(s)	881
AjnI	CCWGG	3 cut(s)	352, 1369, 1940
AluBI	AGCT	3 cut(s)	387, 1526, 1853
AluI	AGCT	3 cut(s)	387, 1526, 1853
Alw21I	GWGCWC	1 cut(s)	574
AlwI	GGATC	7 cut(s)	510, 519, 532, 793, 934, 1574, 1796
AlwNI	CAGNNNCTG	1 cut(s)	1282
Ama87I	CYCGRG	1 cut(s)	1563
AoxI	GGCC	6 cut(s)	49, 677, 743, 868, 1000, 1017
ApeKI	GCWGC	8 cut(s)	290, 349, 757, 777, 955, 958, 1175, 1853
ApoI	RAATTY	2 cut(s)	1040, 1475
AseI	ATTAAT	1 cut(s)	1770
Asp700I	GAANNNNTTC	2 cut(s)	1155, 1403
Asp718I	GGTACC	1 cut(s)	542
AspLEI	GCGC	2 cut(s)	28, 1293
AspS9I	GGNCC	4 cut(s)	50, 148, 1000, 1194
AsuC2I	CCSGG	1 cut(s)	1033
AsuHPI	GGTGA	1 cut(s)	1225
AvaI	CYCGRG	1 cut(s)	1563
AvaII	GGWCC	2 cut(s)	148, 1194
BalI	TGGCCA	1 cut(s)	1019
BamHI	GGATCC	1 cut(s)	524
BanI	GGYRCC	1 cut(s)	542
BarI	GAAGNNNNNNTAC	2 cut(s)	655, 687
BbsI	GAAGAC	1 cut(s)	1421
Bbv12I	GWGCWC	1 cut(s)	574
BbvI	GCAGC	8 cut(s)	277, 361, 744, 789, 967, 970, 1187, 1840
BccI	CCATC	7 cut(s)	72, 430, 484, 493, 1051, 1123, 1185
BceAI	ACGGC	1 cut(s)	968
BcgI	CGANNNNNNTGC	4 cut(s)	1247, 1281, 1862, 1896
BciT130I	CCWGG	3 cut(s)	354, 1371, 1942
BciVI	GTATCC	1 cut(s)	1852
BclI	TGATCA	6 cut(s)	259, 337, 709, 910, 1135, 1252
BcnI	CCSGG	1 cut(s)	1033
BcuI	ACTAGT	1 cut(s)	881
BfaI	CTAG	6 cut(s)	68, 153, 675, 774, 882, 1649
BfmI	CTRYAG	1 cut(s)	45
BfuI	GTATCC	1 cut(s)	1852
BglII	AGATCT	1 cut(s)	1716
BmcAI	AGTACT	2 cut(s)	670, 970
Bme1390I	CCNGG	4 cut(s)	354, 1033, 1371, 1942
Bme18I	GGWCC	2 cut(s)	148, 1194
BmeT110I	CYCGRG	1 cut(s)	1563
BmgT120I	GGNCC	4 cut(s)	50, 148, 1000, 1194
BmiI	GGNNCC	7 cut(s)	149, 150, 526, 544, 627, 1001, 1886
BmrFI	CCNGG	4 cut(s)	354, 1033, 1371, 1942
BmsI	GCATC	3 cut(s)	286, 927, 970
BoxI	GACNNNNGTC	1 cut(s)	153
BpiI	GAAGAC	1 cut(s)	1421
BpmI	CTGGAG	2 cut(s)	1307, 1752
BpuEI	CTTGAG	2 cut(s)	1220, 1375
BpuMI	CCSGG	1 cut(s)	1033
BsaAI	YACGTR	2 cut(s)	511, 830
BsaBI	GATNNNNATC	2 cut(s)	790, 909
BsaJI	CCNNGG	5 cut(s)	781, 1098, 1422, 1845, 1941
BsaXI	ACNNNNNCTCC	2 cut(s)	652, 682
Bsc4I	CCNNNNNNNGG	3 cut(s)	157, 158, 353
Bse3DI	GCAATG	1 cut(s)	1278
Bse8I	GATNNNNATC	2 cut(s)	790, 909
BseBI	CCWGG	3 cut(s)	354, 1371, 1942
BseDI	CCNNGG	5 cut(s)	781, 1098, 1422, 1845, 1941
BseGI	GGATG	4 cut(s)	422, 733, 961, 1196
BseJI	GATNNNNATC	2 cut(s)	790, 909
BseLI	CCNNNNNNNGG	3 cut(s)	157, 158, 353
BseMI	GCAATG	1 cut(s)	1278
BseMII	CTCAG	2 cut(s)	296, 405
BseRI	GAGGAG	5 cut(s)	30, 458, 461, 657, 1076
BseXI	GCAGC	8 cut(s)	277, 361, 744, 789, 967, 970, 1187, 1840
BseYI	CCCAGC	1 cut(s)	1365
BsgI	GTGCAG	2 cut(s)	933, 1622
Bsh1236I	CGCG	5 cut(s)	111, 600, 783, 1293, 1630
BshFI	GGCC	6 cut(s)	51, 679, 745, 870, 1002, 1019
BshNI	GGYRCC	1 cut(s)	542
BsiHKAI	GWGCWC	1 cut(s)	574
BsiHKCI	CYCGRG	1 cut(s)	1563
BsiSI	CCGG	3 cut(s)	134, 1032, 1073
BslFI	GGGAC	1 cut(s)	161
BslI	CCNNNNNNNGG	3 cut(s)	157, 158, 353
BsmFI	GGGAC	1 cut(s)	161
BsmI	GAATGC	1 cut(s)	1319
BsnI	GGCC	6 cut(s)	51, 679, 745, 870, 1002, 1019
BsoBI	CYCGRG	1 cut(s)	1563
Bsp1286I	GDGCHC	1 cut(s)	574
Bsp68I	TCGCGA	1 cut(s)	111
BspACI	CCGC	9 cut(s)	347, 593, 600, 781, 783, 867, 1112, 1327, 1628
BspANI	GGCC	6 cut(s)	51, 679, 745, 870, 1002, 1019
BspCNI	CTCAG	2 cut(s)	297, 404
BspFNI	CGCG	5 cut(s)	111, 600, 783, 1293, 1630
BspHI	TCATGA	2 cut(s)	334, 907
BspLI	GGNNCC	7 cut(s)	149, 150, 526, 544, 627, 1001, 1886
BspPI	GGATC	7 cut(s)	510, 519, 532, 793, 934, 1574, 1796
BspQI	GCTCTTC	3 cut(s)	579, 1731, 1797
BspT107I	GGYRCC	1 cut(s)	542
BsrDI	GCAATG	1 cut(s)	1278
BssECI	CCNNGG	5 cut(s)	781, 1098, 1422, 1845, 1941
BssNAI	GTATAC	1 cut(s)	1444
BssT1I	CCWWGG	2 cut(s)	1422, 1845
Bst1107I	GTATAC	1 cut(s)	1444
Bst2UI	CCWGG	3 cut(s)	354, 1371, 1942
Bst4CI	ACNGT	2 cut(s)	1314, 1907
Bst6I	CTCTTC	4 cut(s)	5, 579, 1731, 1797
BstAPI	GCANNNNNTGC	1 cut(s)	1282
BstBAI	YACGTR	2 cut(s)	511, 830
BstC8I	GCNNGC	2 cut(s)	598, 1070
BstDEI	CTNAG	4 cut(s)	305, 374, 391, 1489
BstDSI	CCRYGG	1 cut(s)	781
BstF5I	GGATG	4 cut(s)	422, 733, 961, 1196
BstFNI	CGCG	5 cut(s)	111, 600, 783, 1293, 1630
BstHHI	GCGC	2 cut(s)	28, 1293
BstMWI	GCNNNNNNNGC	4 cut(s)	1016, 1282, 1336, 1829
BstNI	CCWGG	3 cut(s)	354, 1371, 1942
BstNSI	RCATGY	1 cut(s)	1745
BstPAI	GACNNNNGTC	1 cut(s)	153
BstSCI	CCNGG	4 cut(s)	352, 1031, 1369, 1940
BstSFI	CTRYAG	1 cut(s)	45
BstSNI	TACGTA	1 cut(s)	830
BstUI	CGCG	5 cut(s)	111, 600, 783, 1293, 1630
BstV1I	GCAGC	8 cut(s)	277, 361, 744, 789, 967, 970, 1187, 1840
BstV2I	GAAGAC	1 cut(s)	1421
BstX2I	RGATCY	4 cut(s)	524, 1579, 1716, 1801
BstYI	RGATCY	4 cut(s)	524, 1579, 1716, 1801
BstZ17I	GTATAC	1 cut(s)	1444
BsuI	GTATCC	1 cut(s)	1852
BsuRI	GGCC	6 cut(s)	51, 679, 745, 870, 1002, 1019
BtgI	CCRYGG	1 cut(s)	781
BtsCI	GGATG	4 cut(s)	422, 733, 961, 1196
BtsIMutI	CAGTG	1 cut(s)	1248
BtuMI	TCGCGA	1 cut(s)	111
Cac8I	GCNNGC	2 cut(s)	598, 1070
CaiI	CAGNNNCTG	1 cut(s)	1282
CciI	TCATGA	2 cut(s)	334, 907
CfoI	GCGC	2 cut(s)	28, 1293
Cfr13I	GGNCC	4 cut(s)	50, 148, 1000, 1194
Cfr42I	CCGCGG	1 cut(s)	784
CseI	GACGC	3 cut(s)	354, 812, 1523
Csp6I	GTAC	7 cut(s)	360, 508, 543, 669, 831, 969, 1606
CspCI	CAANNNNNGTGG	2 cut(s)	1767, 1802
CviQI	GTAC	7 cut(s)	360, 508, 543, 669, 831, 969, 1606
DdeI	CTNAG	4 cut(s)	305, 374, 391, 1489
DrdI	GACNNNNNNGTC	2 cut(s)	68, 822
DseDI	GACNNNNNNGTC	2 cut(s)	68, 822
EaeI	YGGCCR	2 cut(s)	868, 1017
Eam1104I	CTCTTC	4 cut(s)	5, 579, 1731, 1797
EarI	CTCTTC	4 cut(s)	5, 579, 1731, 1797
EciI	GGCGGA	1 cut(s)	1127
Eco105I	TACGTA	1 cut(s)	830
Eco130I	CCWWGG	2 cut(s)	1422, 1845
Eco147I	AGGCCT	1 cut(s)	745
Eco32I	GATATC	1 cut(s)	731
Eco47I	GGWCC	2 cut(s)	148, 1194
Eco57I	CTGAAG	2 cut(s)	1355, 1596
Eco88I	CYCGRG	1 cut(s)	1563
EcoO109I	RGGNCCY	1 cut(s)	148
EcoRII	CCWGG	3 cut(s)	352, 1369, 1940
EcoRV	GATATC	1 cut(s)	731
EcoT14I	CCWWGG	2 cut(s)	1422, 1845
EcoT22I	ATGCAT	1 cut(s)	1685
ErhI	CCWWGG	2 cut(s)	1422, 1845
FalI	AAGNNNNNCTT	2 cut(s)	1521, 1553
FaqI	GGGAC	1 cut(s)	161
FauI	CCCGC	1 cut(s)	1635
FauNDI	CATATG	1 cut(s)	1244
FbaI	TGATCA	6 cut(s)	259, 337, 709, 910, 1135, 1252
FblI	GTMKAC	2 cut(s)	826, 1443
FokI	GGATG	4 cut(s)	409, 720, 948, 1203
FspBI	CTAG	6 cut(s)	68, 153, 675, 774, 882, 1649
GlaI	GCGC	2 cut(s)	27, 1292
GsaI	CCCAGC	1 cut(s)	1369
GsuI	CTGGAG	2 cut(s)	1307, 1752
HaeIII	GGCC	6 cut(s)	51, 679, 745, 870, 1002, 1019
HapII	CCGG	3 cut(s)	134, 1032, 1073
HgaI	GACGC	3 cut(s)	354, 812, 1523
HhaI	GCGC	2 cut(s)	28, 1293
Hin6I	GCGC	2 cut(s)	26, 1291
HinP1I	GCGC	2 cut(s)	26, 1291
HinfI	GANTC	7 cut(s)	113, 298, 472, 649, 1348, 1384, 1415
HpaII	CCGG	3 cut(s)	134, 1032, 1073
HphI	GGTGA	1 cut(s)	1225
Hpy166II	GTNNAC	4 cut(s)	827, 1444, 1759, 1778
Hpy188I	TCNGA	7 cut(s)	264, 981, 1099, 1161, 1335, 1576, 1884
Hpy188III	TCNNGA	9 cut(s)	110, 335, 393, 813, 908, 1412, 1433, 1720, 1799
Hpy8I	GTNNAC	4 cut(s)	827, 1444, 1759, 1778
Hpy99I	CGWCG	2 cut(s)	111, 370
HpyAV	CCTTC	3 cut(s)	113, 129, 1181
HpyCH4III	ACNGT	2 cut(s)	1314, 1907
HpyCH4IV	ACGT	3 cut(s)	165, 510, 829
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1016, 1282, 1336, 1829
HpyF3I	CTNAG	4 cut(s)	305, 374, 391, 1489
HpySE526I	ACGT	3 cut(s)	165, 510, 829
HspAI	GCGC	2 cut(s)	26, 1291
KflI	GGGWCCC	1 cut(s)	148
KpnI	GGTACC	1 cut(s)	546
Ksp22I	TGATCA	6 cut(s)	259, 337, 709, 910, 1135, 1252
KspI	CCGCGG	1 cut(s)	784
LguI	GCTCTTC	3 cut(s)	579, 1731, 1797
LmnI	GCTCC	5 cut(s)	192, 644, 1288, 1850, 1884
Lsp1109I	GCAGC	8 cut(s)	277, 361, 744, 789, 967, 970, 1187, 1840
LweI	GCATC	3 cut(s)	286, 927, 970
MaeI	CTAG	6 cut(s)	68, 153, 675, 774, 882, 1649
MaeII	ACGT	3 cut(s)	165, 510, 829
MaeIII	GTNAC	3 cut(s)	1633, 1946, 1958
MflI	RGATCY	4 cut(s)	524, 1579, 1716, 1801
MhlI	GDGCHC	1 cut(s)	574
MlsI	TGGCCA	1 cut(s)	1019
MluNI	TGGCCA	1 cut(s)	1019
MlyI	GAGTC	1 cut(s)	658
Mox20I	TGGCCA	1 cut(s)	1019
Mph1103I	ATGCAT	1 cut(s)	1685
MroXI	GAANNNNTTC	2 cut(s)	1155, 1403
MscI	TGGCCA	1 cut(s)	1019
MseI	TTAA	6 cut(s)	39, 770, 893, 1619, 1674, 1770
MslI	CAYNNNNRTG	2 cut(s)	333, 912
Msp20I	TGGCCA	1 cut(s)	1019
MspA1I	CMGCKG	2 cut(s)	593, 783
MspI	CCGG	3 cut(s)	134, 1032, 1073
MspR9I	CCNGG	4 cut(s)	354, 1033, 1371, 1942
Mva1269I	GAATGC	1 cut(s)	1319
MvaI	CCWGG	3 cut(s)	354, 1371, 1942
MvnI	CGCG	5 cut(s)	111, 600, 783, 1293, 1630
MwoI	GCNNNNNNNGC	4 cut(s)	1016, 1282, 1336, 1829
NciI	CCSGG	1 cut(s)	1033
NdeI	CATATG	1 cut(s)	1244
NlaIV	GGNNCC	7 cut(s)	149, 150, 526, 544, 627, 1001, 1886
NmuCI	GTSAC	1 cut(s)	1946
NruI	TCGCGA	1 cut(s)	111
NsiI	ATGCAT	1 cut(s)	1685
NspI	RCATGY	1 cut(s)	1745
PaeR7I	CTCGAG	1 cut(s)	1563
PagI	TCATGA	2 cut(s)	334, 907
PceI	AGGCCT	1 cut(s)	745
PciSI	GCTCTTC	3 cut(s)	579, 1731, 1797
PctI	GAATGC	1 cut(s)	1319
PdmI	GAANNNNTTC	2 cut(s)	1155, 1403
PfeI	GAWTC	6 cut(s)	113, 298, 472, 1348, 1384, 1415
PflFI	GACNNNGTC	1 cut(s)	822
PleI	GAGTC	1 cut(s)	657
PpsI	GAGTC	1 cut(s)	657
Ppu21I	YACGTR	2 cut(s)	511, 830
PpuMI	RGGWCCY	1 cut(s)	148
PshAI	GACNNNNGTC	1 cut(s)	153
PshBI	ATTAAT	1 cut(s)	1770
Psp1406I	AACGTT	1 cut(s)	165
Psp5II	RGGWCCY	1 cut(s)	148
Psp6I	CCWGG	3 cut(s)	352, 1369, 1940
PspFI	CCCAGC	1 cut(s)	1365
PspGI	CCWGG	3 cut(s)	352, 1369, 1940
PspN4I	GGNNCC	7 cut(s)	149, 150, 526, 544, 627, 1001, 1886
PspPI	GGNCC	4 cut(s)	50, 148, 1000, 1194
PspPPI	RGGWCCY	1 cut(s)	148
PspXI	VCTCGAGB	1 cut(s)	1563
PstNI	CAGNNNCTG	1 cut(s)	1282
PsuI	RGATCY	4 cut(s)	524, 1579, 1716, 1801
PsyI	GACNNNGTC	1 cut(s)	822
RruI	TCGCGA	1 cut(s)	111
RsaI	GTAC	7 cut(s)	361, 509, 544, 670, 832, 970, 1607
RsaNI	GTAC	7 cut(s)	360, 508, 543, 669, 831, 969, 1606
RseI	CAYNNNNRTG	2 cut(s)	333, 912
SacII	CCGCGG	1 cut(s)	784
SapI	GCTCTTC	3 cut(s)	579, 1731, 1797
SaqAI	TTAA	6 cut(s)	39, 770, 893, 1619, 1674, 1770
Sau96I	GGNCC	4 cut(s)	50, 148, 1000, 1194
ScaI	AGTACT	2 cut(s)	670, 970
SchI	GAGTC	1 cut(s)	658
ScrFI	CCNGG	4 cut(s)	354, 1033, 1371, 1942
SduI	GDGCHC	1 cut(s)	574
SfaNI	GCATC	3 cut(s)	286, 927, 970
SfcI	CTRYAG	1 cut(s)	45
Sfr274I	CTCGAG	1 cut(s)	1563
Sfr303I	CCGCGG	1 cut(s)	784
SgrBI	CCGCGG	1 cut(s)	784
SinI	GGWCC	2 cut(s)	148, 1194
SlaI	CTCGAG	1 cut(s)	1563
SmiMI	CAYNNNNRTG	2 cut(s)	333, 912
SmlI	CTYRAG	3 cut(s)	1235, 1354, 1563
SmoI	CTYRAG	3 cut(s)	1235, 1354, 1563
SnaBI	TACGTA	1 cut(s)	830
SpeI	ACTAGT	1 cut(s)	881
SseBI	AGGCCT	1 cut(s)	745
SsiI	CCGC	9 cut(s)	347, 593, 600, 781, 783, 867, 1112, 1327, 1628
SspI	AATATT	2 cut(s)	455, 688
SspMI	CTAG	6 cut(s)	68, 153, 675, 774, 882, 1649
StuI	AGGCCT	1 cut(s)	745
StyD4I	CCNGG	4 cut(s)	352, 1031, 1369, 1940
StyI	CCWWGG	2 cut(s)	1422, 1845
TaaI	ACNGT	2 cut(s)	1314, 1907
TaiI	ACGT	3 cut(s)	168, 513, 832
TaqI	TCGA	5 cut(s)	301, 314, 368, 1267, 1564
TatI	WGTACW	3 cut(s)	668, 968, 1605
TauI	GCSGC	5 cut(s)	349, 596, 783, 870, 1330
TfiI	GAWTC	6 cut(s)	113, 298, 472, 1348, 1384, 1415
Tru1I	TTAA	6 cut(s)	39, 770, 893, 1619, 1674, 1770
Tru9I	TTAA	6 cut(s)	39, 770, 893, 1619, 1674, 1770
TscAI	CASTG	1 cut(s)	1255
TseFI	GTSAC	1 cut(s)	1946
TseI	GCWGC	8 cut(s)	290, 349, 757, 777, 955, 958, 1175, 1853
Tsp45I	GTSAC	1 cut(s)	1946
TspDTI	ATGAA	5 cut(s)	23, 509, 1312, 1496, 1700
TspGWI	ACGGA	3 cut(s)	780, 1109, 1275
TspRI	CASTG	1 cut(s)	1255
Tth111I	GACNNNGTC	1 cut(s)	822
VpaK11BI	GGWCC	2 cut(s)	148, 1194
VspI	ATTAAT	1 cut(s)	1770
XapI	RAATTY	2 cut(s)	1040, 1475
XceI	RCATGY	1 cut(s)	1745
XcmI	CCANNNNNNNNNTGG	2 cut(s)	563, 1429
XhoI	CTCGAG	1 cut(s)	1563
XmiI	GTMKAC	2 cut(s)	826, 1443
XmnI	GAANNNNTTC	2 cut(s)	1155, 1403
XspI	CTAG	6 cut(s)	68, 153, 675, 774, 882, 1649
ZrmI	AGTACT	2 cut(s)	670, 970
Zsp2I	ATGCAT	1 cut(s)	1685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.