RLG00000014133

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004233
Physical Location & Seq
Forward (+)
47834374 .. 47835547
1174 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014133

Sequence Viewer

Length: 993 bp
ATGTTGCCCAATCTCGTTCTTCAAAACCCACCAATCTTGCAAAACCCTCCTTACCATCAAAACACTAATCCCAACTCTGATCATCTTCTTTTTCCCGGAAACAGTACCAACAATCTTATCAACAACAATAATAATGAGTTTTCGACAGTGGGATTTAACAGTAACAATGAGTTTTCGACATTGGGATTTGGAACAGGTGGGGGAGGTCCTCATGATTTTGCTGCTGATCATACTCATGATCAACTCACAGCTTCTTTGAATCGGCTAAGCATATCCAATAATGGCAGTTCAGGCAATCAAAGAATACCCAATAACTATATGCCTAGCTCTACAATCTTAGGCAATGCTGGGTTCAGTACTCCAGAGCAAGAATATTTGGAGAGGTTGAACCAATATGCTTTCTTAATGGCTGAGCAGTACATGAGGCTTTCGAATCCACTATTGCTAACAATAGAATACTCAATCCCCATAGTTGGAATCGGAATAGCACCACGGAATGGGGTTAGCAGGGGATCAAGGTTTGCTAATGGGGCTGGAAGACAGCGTTTCGGGCAAGAAGAAAGGAGCCAAGACCTGAATTTGATGTCCAGCAAAGATCAAAGGCTTGGCAATAGGTCTGCACGGCGGCATATACCAAGTTATTACTCTATCCCTTTAAAAGAGTTGAGGGGCCAAATTGCTTTTATGGTCAAAGATCATGTTGGATGTCGATTTTTACAAAAGAAGATTGAGGAGGGTAAGATGGAAGAGATTGAAATGATTTTTAATGAGGTTATGAAGGAGGGTCATGATCGTTTGCGCGAGCTAATGGTAGATCAATTTGGGACTGAATTTATTCAGAAACTTGTGGAGGGGACTAACAGGGACGGATGGACCAAAATGCTTGATTTGGTGATGAGTGATGAGAGGAAACTCACGGATATGTGCACTGACCAGCATGGAAGTCGTGCCATGCAGAAGTTGCTGGAGAGGGTTGAAATCCGAGAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF zf-CCCH

Protein Analysis

331

Amino Acids

37.35

Weight (kDa)

6.16

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 231 - 259 3.1e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 497
AccII CGCG 1 cut(s) 801
AciI CCGC 1 cut(s) 625
AclWI GGATC 1 cut(s) 520
AcsI RAATTY 2 cut(s) 577, 830
AfaI GTAC 3 cut(s) 106, 358, 419
AfiI CCNNNNNNNGG 2 cut(s) 473, 497
AgsI TTSAA 5 cut(s) 23, 259, 388, 755, 977
AluBI AGCT 3 cut(s) 251, 327, 805
AluI AGCT 3 cut(s) 251, 327, 805
Alw21I GWGCWC 1 cut(s) 929
Alw44I GTGCAC 1 cut(s) 925
AlwI GGATC 1 cut(s) 520
AoxI GGCC 1 cut(s) 670
ApaLI GTGCAC 1 cut(s) 925
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 2 cut(s) 577, 830
Asp700I GAANNNNTTC 1 cut(s) 834
AspLEI GCGC 1 cut(s) 801
AspS9I GGNCC 3 cut(s) 206, 670, 873
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 904
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 2 cut(s) 206, 873
BaeGI GKGCMC 1 cut(s) 929
BbsI GAAGAC 1 cut(s) 544
Bbv12I GWGCWC 1 cut(s) 929
BbvI GCAGC 1 cut(s) 208
BccI CCATC 3 cut(s) 63, 736, 864
BceAI ACGGC 1 cut(s) 638
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BclI TGATCA 3 cut(s) 79, 226, 238
BcnI CCSGG 1 cut(s) 96
BfaI CTAG 1 cut(s) 324
BisI GCNGC 2 cut(s) 222, 626
BlpI GCTNAGC 2 cut(s) 266, 411
BlsI GCNGC 2 cut(s) 223, 627
BmcAI AGTACT 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 2 cut(s) 206, 873
BmgT120I GGNCC 3 cut(s) 206, 670, 873
BmiI GGNNCC 2 cut(s) 566, 671
BmrFI CCNGG 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 544
BpmI CTGGAG 2 cut(s) 345, 986
Bpu1102I GCTNAGC 2 cut(s) 266, 411
Bpu14I TTCGAA 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 491
Bsc4I CCNNNNNNNGG 2 cut(s) 473, 497
Bse3DI GCAATG 1 cut(s) 349
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 2 cut(s) 710, 875
BseLI CCNNNNNNNGG 2 cut(s) 473, 497
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 746
BseSI GKGCMC 1 cut(s) 929
BseXI GCAGC 1 cut(s) 208
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 603
Bsh1236I CGCG 1 cut(s) 801
BshFI GGCC 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 929
BsiSI CCGG 1 cut(s) 96
BslFI GGGAC 3 cut(s) 838, 868, 878
BslI CCNNNNNNNGG 2 cut(s) 473, 497
BsmFI GGGAC 3 cut(s) 838, 868, 878
BsnI GGCC 1 cut(s) 672
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 929
Bsp143I GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
Bsp1720I GCTNAGC 2 cut(s) 266, 411
BspACI CCGC 1 cut(s) 625
BspANI GGCC 1 cut(s) 672
BspCNI CTCAG 1 cut(s) 403
BspFNI CGCG 1 cut(s) 801
BspHI TCATGA 3 cut(s) 211, 235, 787
BspLI GGNNCC 2 cut(s) 566, 671
BspPI GGATC 1 cut(s) 520
BspT104I TTCGAA 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
Bst4CI ACNGT 3 cut(s) 104, 148, 161
Bst6I CTCTTC 1 cut(s) 741
BstAPI GCANNNNNTGC 1 cut(s) 961
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 803
BstDEI CTNAG 3 cut(s) 266, 337, 411
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 2 cut(s) 710, 875
BstFNI CGCG 1 cut(s) 801
BstHHI GCGC 1 cut(s) 801
BstKTI GATC 8 cut(s) 82, 229, 241, 515, 598, 697, 793, 817
BstMBI GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
BstMWI GCNNNNNNNGC 4 cut(s) 291, 530, 550, 961
BstSCI CCNGG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 929
BstUI CGCG 1 cut(s) 801
BstV1I GCAGC 1 cut(s) 208
BstV2I GAAGAC 1 cut(s) 544
BsuRI GGCC 1 cut(s) 672
BtgI CCRYGG 1 cut(s) 491
BtsCI GGATG 2 cut(s) 710, 875
BtsIMutI CAGTG 2 cut(s) 153, 927
Cac8I GCNNGC 1 cut(s) 803
CciI TCATGA 3 cut(s) 211, 235, 787
CfoI GCGC 1 cut(s) 801
Cfr13I GGNCC 3 cut(s) 206, 670, 873
Csp6I GTAC 3 cut(s) 105, 357, 418
CspCI CAANNNNNGTGG 2 cut(s) 18, 53
CviAII CATG 7 cut(s) 212, 236, 421, 698, 788, 938, 952
CviQI GTAC 3 cut(s) 105, 357, 418
DdeI CTNAG 3 cut(s) 266, 337, 411
DpnI GATC 8 cut(s) 81, 228, 240, 514, 597, 696, 792, 816
DpnII GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
DraI TTTAAA 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Eco47I GGWCC 2 cut(s) 206, 873
EcoO109I RGGNCCY 1 cut(s) 206
FaeI CATG 7 cut(s) 215, 239, 424, 701, 791, 941, 955
FaqI GGGAC 3 cut(s) 838, 868, 878
FatI CATG 7 cut(s) 211, 235, 420, 697, 787, 937, 951
FbaI TGATCA 3 cut(s) 79, 226, 238
Fnu4HI GCNGC 2 cut(s) 222, 626
FokI GGATG 2 cut(s) 717, 882
Fsp4HI GCNGC 2 cut(s) 222, 626
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 800
GluI GCNGC 2 cut(s) 222, 626
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 2 cut(s) 345, 986
HaeIII GGCC 1 cut(s) 672
HapII CCGG 1 cut(s) 96
HhaI GCGC 1 cut(s) 801
Hin1II CATG 7 cut(s) 215, 239, 424, 701, 791, 941, 955
Hin6I GCGC 1 cut(s) 799
HinP1I GCGC 1 cut(s) 799
HinfI GANTC 3 cut(s) 259, 433, 477
HpaII CCGG 1 cut(s) 96
HphI GGTGA 1 cut(s) 904
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 4 cut(s) 79, 482, 840, 983
Hpy188III TCNNGA 4 cut(s) 212, 236, 362, 788
Hpy8I GTNNAC 1 cut(s) 927
HpyAV CCTTC 1 cut(s) 772
HpyCH4III ACNGT 3 cut(s) 104, 148, 161
HpyCH4V TGCA 4 cut(s) 40, 620, 927, 955
HpyF10VI GCNNNNNNNGC 4 cut(s) 291, 530, 550, 961
HpyF3I CTNAG 3 cut(s) 266, 337, 411
Hsp92II CATG 7 cut(s) 215, 239, 424, 701, 791, 941, 955
HspAI GCGC 1 cut(s) 799
Ksp22I TGATCA 3 cut(s) 79, 226, 238
Kzo9I GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 1 cut(s) 208
MaeI CTAG 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 161
MalI GATC 8 cut(s) 81, 228, 240, 514, 597, 696, 792, 816
MboI GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
MboII GAAGA 6 cut(s) 11, 77, 549, 569, 736, 758
MhlI GDGCHC 1 cut(s) 929
MluCI AATT 4 cut(s) 577, 675, 818, 830
MmeI TCCRAC 2 cut(s) 454, 682
MroXI GAANNNNTTC 1 cut(s) 834
MseI TTAA 4 cut(s) 156, 404, 656, 765
MslI CAYNNNNRTG 2 cut(s) 234, 920
MspI CCGG 1 cut(s) 96
MspR9I CCNGG 1 cut(s) 96
MvnI CGCG 1 cut(s) 801
MwoI GCNNNNNNNGC 4 cut(s) 291, 530, 550, 961
NciI CCSGG 1 cut(s) 96
NdeII GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
NlaIII CATG 7 cut(s) 215, 239, 424, 701, 791, 941, 955
NlaIV GGNNCC 2 cut(s) 566, 671
NspV TTCGAA 1 cut(s) 431
PagI TCATGA 3 cut(s) 211, 235, 787
PdmI GAANNNNTTC 1 cut(s) 834
PfeI GAWTC 3 cut(s) 259, 433, 477
PflMI CCANNNNNTGG 1 cut(s) 497
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 2 cut(s) 223, 627
PpuMI RGGWCCY 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 206
PspFI CCCAGC 1 cut(s) 347
PspN4I GGNNCC 2 cut(s) 566, 671
PspPI GGNCC 3 cut(s) 206, 670, 873
PspPPI RGGWCCY 1 cut(s) 206
RsaI GTAC 3 cut(s) 106, 358, 419
RsaNI GTAC 3 cut(s) 105, 357, 418
RseI CAYNNNNRTG 2 cut(s) 234, 920
SaqAI TTAA 4 cut(s) 156, 404, 656, 765
SatI GCNGC 2 cut(s) 222, 626
Sau3AI GATC 8 cut(s) 79, 226, 238, 512, 595, 694, 790, 814
Sau96I GGNCC 3 cut(s) 206, 670, 873
ScaI AGTACT 1 cut(s) 358
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 1 cut(s) 929
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 2 cut(s) 206, 873
SmiMI CAYNNNNRTG 2 cut(s) 234, 920
Sse9I AATT 4 cut(s) 577, 675, 818, 830
SsiI CCGC 1 cut(s) 625
SspI AATATT 1 cut(s) 374
SspMI CTAG 1 cut(s) 324
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 3 cut(s) 104, 148, 161
TaqI TCGA 4 cut(s) 143, 176, 431, 709
TasI AATT 4 cut(s) 577, 675, 818, 830
TatI WGTACW 2 cut(s) 356, 417
TauI GCSGC 1 cut(s) 628
TfiI GAWTC 3 cut(s) 259, 433, 477
Tru1I TTAA 4 cut(s) 156, 404, 656, 765
Tru9I TTAA 4 cut(s) 156, 404, 656, 765
TscAI CASTG 2 cut(s) 153, 934
TseI GCWGC 1 cut(s) 221
TspDTI ATGAA 1 cut(s) 791
TspGWI ACGGA 3 cut(s) 508, 882, 932
TspRI CASTG 2 cut(s) 153, 934
Van91I CCANNNNNTGG 1 cut(s) 497
VneI GTGCAC 1 cut(s) 925
VpaK11BI GGWCC 2 cut(s) 206, 873
XapI RAATTY 2 cut(s) 577, 830
XmnI GAANNNNTTC 1 cut(s) 834
XspI CTAG 1 cut(s) 324
ZrmI AGTACT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.