Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0004233

Physical Location & Seq

Genomic Coordinates Reverse (-)

9923758 .. 9928233

Gene Structure 4476 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG068500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGTATTGGGATCCCATGTTGCCCAATCAGGTTCTTCAAAACCCACCAATCTTGCAAAACCCTCCCTACCATCAAAACACCAACCCCAACTTTGATCATCTTCTTTTTCCCGGAAACAATACCAACAATCTTATCAACAACAACAATTATGGGTTTTCGACAGTGGCATTTAACAGTAACAATGAGTTTTCGACATTGGGATTTGGAACAGGTGGGGGAGGTCCTCATGATTTTGCTGCTGATCAGACTCATGATCAACTCGCAGCTTCTTTGAATCGGCTCACCATATCCAATAATGGCACTTCAGGACCCAACAACTATATGCCTAGCTCTACAATCCTAGGCAATGGTGGGTTCAGTACTCTAGAGCAGGAATATTTGGAGAGGTTGAACCAATATGCTTTCTTAATGGCTGAGCAGTACATGAGGCGTTCGAATCCCACTACTGCTAACAATGGAATACTCAATCCCCATAGTTGGAATCGGAATAGCACCAGTAGTAGTAGGAGCACTAGTCAATCGATTTATGGGGATCAATTTGGATTTGGTTTTAACCAAATTAGGTCTAGTTCTAATTCTCAGCGGAATGGGGTTAGCAGGGGATCAAGGTTTGCTAACGGGACTGGAAGACAGCGTTTTGGGCAAGAAAAAAGGAGCCAAGGCCTGAATTTGATGTCCACCAAAGATCAAAGGCTTGGCAATGGGTCTACACGGCGGCATATACCAAGTTATTACTCTGTCCCATTGGAAGAGTTGAGGGGCCAAATTGCTTTTGTGGCCAAAGATCATGTTGGATGTCGATTTTTACAAAAGAAGATTGAGGAGGGTAAGATGGAAGAGATTGAAATGATTTTTAATGAGGTTATGAAGGAGGGTCATGATCATTTGCGCGAGCTAATGGTAGATCAATTTGGGACTGAATTTATTCAAAAACTTGTGGAGGGGACTAACAGAGACGGATGGACCAAAATGCTTGATTTGGTGATGAGTGATGAGAGGAAACTCAAGGATATGTGCACTGACCAGCATGGAAGTCGTGCCATGCAGAAGTTGCTGGAGCGGGTTGAAACCCGAGAGCAACAGGCCTCGTTTATGCGGGTTCTGAAGCGAATCACAATTCCGCTGAGCAAAACCCAGCCTGGTTATTATGTGATTCAAGTTTGCTTGAAAAATTTCTCTCCTGACTGCACCAAGGGTGTTCTGGAATTAGTGCTAGATAACTGCCTTGCATTAGCAAAAGACAAATTTGGGTGTTGCTTAGTGCAAAGCAGTGTGCATTATGCGTATACTGAAGCTAAGGAGCGTCTTGTGGCTGACATAACTGAGCATGCTCGAGTCCTCTCTGAAGATCCTTACGGGAACTATGTTGTGCAGTACATAATTGGACTAAAAATACCTCGTGTAACAGCAGACATTTTAGGTCAGCTTCAGGGGTATTTTGTTAATTTGTCAATGAACAAGCATGGAAGTAATGTAGTTGAGAAAACCCTGAAAGAGGCTGGAGAAGAACATGCCAATATGATTATTAATGAGATGATTAACAGTCCTGAGTTTCTGAATGTTCTTCAAAATCCTTATGGGAATTATGTTGCTCAGTCTGCTCTTGCAGTATCCAAGGGATCTGTTCACAATGCTCTTGTTAACCTCATTCGGAGCAATTACCCTCATCTGCACAGCCATCTGTATGGGAAAAGGGTGCTTGACAAAACCAGAGGAGGCAGACATCGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PUF zf-CCCH

Protein Analysis

577

Amino Acids

65.0

Weight (kDa)

8.63

Isoelectric Point (pI)

41.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUF	PF00806	258 - 289	7.8e-08	Pumilio-family RNA binding repeat
PUF	PF00806	407 - 440	4.1e-06	Pumilio-family RNA binding repeat
PUF	PF00806	479 - 513	5.7e-08	Pumilio-family RNA binding repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004233)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g30780 FvH4_5g30780
malus_domestica	MD05G1093500.v1.1
prunus_persica	Prupe.8G138200_v2.0.a1 Prupe.8G138200_v2.0.a1
pyrus_communis	pycom15g06920
rosa_chinensis	RchiOBHm_Chr6g0254321 RchiOBHm_Chr6g0254351
rosa_laevigata	RLG00000014133 RLG00000014918 RLG00000014919 RLG00000014923 RLG00000014926
rosa_multiflora	Rmu_sc0006565.1_g000002 Rmu_sc0007138.1_g000009 Rmu_sc0027609.1_g000002
rosa_roxburghii	Rroxscaffold_7G00210870 Rroxscaffold_7G00210910 Rroxscaffold_7G00210930
rosa_rugosa	Rorug05G0552000
rosa_samantha	Rh6AG068500 Rh6AG068800 Rh6AG068900 Rh6BG061200 Rh6CG061000 Rh6CG061100 Rh6CG061500 Rh6DG058000 Rh6DG058300 Rh6DG058400 Rh6DG058700
rosa_wichuraiana	Rw6G006000 Rw6G006040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1060
AccI	GTMKAC	2 cut(s)	707, 1286
AccII	CGCG	1 cut(s)	891
AciI	CCGC	5 cut(s)	583, 715, 1060, 1096, 1121
AclWI	GGATC	6 cut(s)	5, 18, 540, 610, 1343, 1627
AcoI	YGGCCR	1 cut(s)	777
AcsI	RAATTY	4 cut(s)	667, 920, 1171, 1244
AcuI	CTGAAG	5 cut(s)	288, 1124, 1311, 1365, 1412
AfaI	GTAC	3 cut(s)	361, 422, 1376
AfiI	CCNNNNNNNGG	2 cut(s)	477, 1495
AgsI	TTSAA	9 cut(s)	38, 274, 391, 845, 929, 1067, 1157, 1168, 1568
AhlI	ACTAGT	1 cut(s)	512
AjnI	CCWGG	1 cut(s)	1138
AluBI	AGCT	5 cut(s)	266, 330, 895, 1295, 1426
AluI	AGCT	5 cut(s)	266, 330, 895, 1295, 1426
Alw21I	GWGCWC	2 cut(s)	512, 1019
Alw26I	GTCTC	1 cut(s)	948
Alw44I	GTGCAC	1 cut(s)	1015
AlwI	GGATC	6 cut(s)	5, 18, 540, 610, 1343, 1627
Ama87I	CYCGRG	2 cut(s)	1071, 1332
AoxI	GGCC	4 cut(s)	661, 760, 777, 1083
ApaLI	GTGCAC	1 cut(s)	1015
ApeKI	GCWGC	2 cut(s)	236, 263
ApoI	RAATTY	4 cut(s)	667, 920, 1171, 1244
AseI	ATTAAT	1 cut(s)	1527
Asp700I	GAANNNNTTC	2 cut(s)	924, 1172
AspA2I	CCTAGG	1 cut(s)	340
AspLEI	GCGC	1 cut(s)	891
AspS9I	GGNCC	4 cut(s)	221, 308, 760, 963
AsuC2I	CCSGG	1 cut(s)	111
AsuHPI	GGTGA	2 cut(s)	274, 994
AsuII	TTCGAA	1 cut(s)	434
AvaI	CYCGRG	2 cut(s)	1071, 1332
AvaII	GGWCC	3 cut(s)	221, 308, 963
AvrII	CCTAGG	1 cut(s)	340
BaeGI	GKGCMC	1 cut(s)	1019
BalI	TGGCCA	1 cut(s)	779
BamHI	GGATCC	1 cut(s)	10
BauI	CACGAG	1 cut(s)	1398
BbsI	GAAGAC	1 cut(s)	634
Bbv12I	GWGCWC	2 cut(s)	512, 1019
BbvI	GCAGC	2 cut(s)	223, 275
BccI	CCATC	4 cut(s)	78, 826, 954, 1686
BceAI	ACGGC	1 cut(s)	728
BcgI	CGANNNNNNTGC	2 cut(s)	1016, 1050
BciT130I	CCWGG	1 cut(s)	1140
BciVI	GTATCC	1 cut(s)	1621
BclI	TGATCA	4 cut(s)	94, 241, 253, 880
BcnI	CCSGG	1 cut(s)	111
BcoDI	GTCTC	1 cut(s)	948
BcuI	ACTAGT	1 cut(s)	512
BfaI	CTAG	6 cut(s)	327, 341, 365, 513, 567, 1214
BfuI	GTATCC	1 cut(s)	1621
BisI	GCNGC	3 cut(s)	237, 264, 716
BlnI	CCTAGG	1 cut(s)	340
BlpI	GCTNAGC	2 cut(s)	414, 1124
BlsI	GCNGC	3 cut(s)	238, 265, 717
BmcAI	AGTACT	1 cut(s)	361
Bme1390I	CCNGG	2 cut(s)	111, 1140
Bme18I	GGWCC	3 cut(s)	221, 308, 963
BmeT110I	CYCGRG	2 cut(s)	1071, 1332
BmgT120I	GGNCC	4 cut(s)	221, 308, 760, 963
BmiI	GGNNCC	4 cut(s)	12, 310, 656, 761
BmrFI	CCNGG	2 cut(s)	111, 1140
BpiI	GAAGAC	1 cut(s)	634
BpmI	CTGGAG	2 cut(s)	1076, 1521
Bpu10I	CCTNAGC	1 cut(s)	1296
Bpu1102I	GCTNAGC	2 cut(s)	414, 1124
Bpu14I	TTCGAA	1 cut(s)	434
BpuEI	CTTGAG	1 cut(s)	989
BpuMI	CCSGG	1 cut(s)	111
Bsa29I	ATCGAT	1 cut(s)	521
BsaJI	CCNNGG	4 cut(s)	340, 658, 1191, 1614
BsaXI	ACNNNNNCTCC	2 cut(s)	499, 529
Bsc4I	CCNNNNNNNGG	2 cut(s)	477, 1495
Bse1I	ACTGG	2 cut(s)	495, 628
Bse3DI	GCAATG	2 cut(s)	352, 706
BseBI	CCWGG	1 cut(s)	1140
BseCI	ATCGAT	1 cut(s)	521
BseDI	CCNNGG	4 cut(s)	340, 658, 1191, 1614
BseGI	GGATG	2 cut(s)	800, 965
BseLI	CCNNNNNNNGG	2 cut(s)	477, 1495
BseMI	GCAATG	2 cut(s)	352, 706
BseMII	CTCAG	6 cut(s)	405, 593, 1115, 1314, 1539, 1607
BseNI	ACTGG	2 cut(s)	495, 628
BseRI	GAGGAG	2 cut(s)	836, 1728
BseSI	GKGCMC	1 cut(s)	1019
BseXI	GCAGC	2 cut(s)	223, 275
BseYI	CCCAGC	1 cut(s)	1134
BsgI	GTGCAG	3 cut(s)	1171, 1391, 1655
Bsh1236I	CGCG	1 cut(s)	891
BshFI	GGCC	4 cut(s)	663, 762, 779, 1085
BshVI	ATCGAT	1 cut(s)	521
BsiHKAI	GWGCWC	2 cut(s)	512, 1019
BsiHKCI	CYCGRG	2 cut(s)	1071, 1332
BsiSI	CCGG	1 cut(s)	111
BslFI	GGGAC	4 cut(s)	634, 725, 928, 958
BslI	CCNNNNNNNGG	2 cut(s)	477, 1495
BsmAI	GTCTC	1 cut(s)	948
BsmBI	CGTCTC	1 cut(s)	948
BsmFI	GGGAC	4 cut(s)	634, 725, 928, 958
BsnI	GGCC	4 cut(s)	663, 762, 779, 1085
BsoBI	CYCGRG	2 cut(s)	1071, 1332
Bsp119I	TTCGAA	1 cut(s)	434
Bsp1286I	GDGCHC	2 cut(s)	512, 1019
Bsp1720I	GCTNAGC	2 cut(s)	414, 1124
BspACI	CCGC	5 cut(s)	583, 715, 1060, 1096, 1121
BspANI	GGCC	4 cut(s)	663, 762, 779, 1085
BspCNI	CTCAG	6 cut(s)	406, 592, 1116, 1315, 1540, 1606
BspDI	ATCGAT	1 cut(s)	521
BspFNI	CGCG	1 cut(s)	891
BspHI	TCATGA	3 cut(s)	226, 250, 877
BspLI	GGNNCC	4 cut(s)	12, 310, 656, 761
BspPI	GGATC	6 cut(s)	5, 18, 540, 610, 1343, 1627
BspT104I	TTCGAA	1 cut(s)	434
BsrBI	CCGCTC	1 cut(s)	1060
BsrDI	GCAATG	2 cut(s)	352, 706
BsrI	ACTGG	2 cut(s)	495, 628
BssECI	CCNNGG	4 cut(s)	340, 658, 1191, 1614
BssNAI	GTATAC	1 cut(s)	1287
BssSI	CACGAG	1 cut(s)	1398
BssT1I	CCWWGG	4 cut(s)	340, 658, 1191, 1614
Bst1107I	GTATAC	1 cut(s)	1287
Bst2BI	CACGAG	1 cut(s)	1398
Bst2UI	CCWGG	1 cut(s)	1140
Bst4CI	ACNGT	3 cut(s)	163, 176, 1544
Bst6I	CTCTTC	2 cut(s)	744, 831
BstAPI	GCANNNNNTGC	1 cut(s)	1051
BstBI	TTCGAA	1 cut(s)	434
BstC8I	GCNNGC	2 cut(s)	893, 1329
BstDEI	CTNAG	8 cut(s)	414, 579, 1124, 1258, 1296, 1323, 1548, 1593
BstENI	CCTNNNNNAGG	1 cut(s)	1493
BstF5I	GGATG	2 cut(s)	800, 965
BstFNI	CGCG	1 cut(s)	891
BstHHI	GCGC	1 cut(s)	891
BstMAI	GTCTC	1 cut(s)	948
BstMWI	GCNNNNNNNGC	4 cut(s)	640, 776, 1051, 1598
BstNI	CCWGG	1 cut(s)	1140
BstNSI	RCATGY	2 cut(s)	1331, 1514
BstSCI	CCNGG	2 cut(s)	109, 1138
BstSLI	GKGCMC	1 cut(s)	1019
BstUI	CGCG	1 cut(s)	891
BstV1I	GCAGC	2 cut(s)	223, 275
BstV2I	GAAGAC	1 cut(s)	634
BstX2I	RGATCY	3 cut(s)	10, 1348, 1619
BstXI	CCANNNNNNTGG	1 cut(s)	1685
BstYI	RGATCY	3 cut(s)	10, 1348, 1619
BstZ17I	GTATAC	1 cut(s)	1287
Bsu15I	ATCGAT	1 cut(s)	521
BsuI	GTATCC	1 cut(s)	1621
BsuRI	GGCC	4 cut(s)	663, 762, 779, 1085
BsuTUI	ATCGAT	1 cut(s)	521
BtsCI	GGATG	2 cut(s)	800, 965
BtsI	GCAGTG	1 cut(s)	1276
BtsIMutI	CAGTG	3 cut(s)	168, 1017, 1276
Cac8I	GCNNGC	2 cut(s)	893, 1329
CciI	TCATGA	3 cut(s)	226, 250, 877
CfoI	GCGC	1 cut(s)	891
Cfr13I	GGNCC	4 cut(s)	221, 308, 760, 963
ClaI	ATCGAT	1 cut(s)	521
CseI	GACGC	1 cut(s)	1292
Csp6I	GTAC	3 cut(s)	360, 421, 1375
CspCI	CAANNNNNGTGG	2 cut(s)	33, 68
CviQI	GTAC	3 cut(s)	360, 421, 1375
DdeI	CTNAG	8 cut(s)	414, 579, 1124, 1258, 1296, 1323, 1548, 1593
EaeI	YGGCCR	1 cut(s)	777
Eam1104I	CTCTTC	2 cut(s)	744, 831
EarI	CTCTTC	2 cut(s)	744, 831
Eco130I	CCWWGG	4 cut(s)	340, 658, 1191, 1614
Eco147I	AGGCCT	2 cut(s)	663, 1085
Eco47I	GGWCC	3 cut(s)	221, 308, 963
Eco57I	CTGAAG	5 cut(s)	288, 1124, 1311, 1365, 1412
Eco88I	CYCGRG	2 cut(s)	1071, 1332
EcoNI	CCTNNNNNAGG	1 cut(s)	1493
EcoO109I	RGGNCCY	2 cut(s)	221, 308
EcoRII	CCWGG	1 cut(s)	1138
EcoT14I	CCWWGG	4 cut(s)	340, 658, 1191, 1614
ErhI	CCWWGG	4 cut(s)	340, 658, 1191, 1614
Esp3I	CGTCTC	1 cut(s)	948
FaqI	GGGAC	4 cut(s)	634, 725, 928, 958
FauI	CCCGC	2 cut(s)	1053, 1089
FbaI	TGATCA	4 cut(s)	94, 241, 253, 880
FblI	GTMKAC	2 cut(s)	707, 1286
Fnu4HI	GCNGC	3 cut(s)	237, 264, 716
FokI	GGATG	2 cut(s)	807, 972
Fsp4HI	GCNGC	3 cut(s)	237, 264, 716
FspBI	CTAG	6 cut(s)	327, 341, 365, 513, 567, 1214
GlaI	GCGC	1 cut(s)	890
GluI	GCNGC	3 cut(s)	237, 264, 716
GsaI	CCCAGC	1 cut(s)	1138
GsuI	CTGGAG	2 cut(s)	1076, 1521
HaeIII	GGCC	4 cut(s)	663, 762, 779, 1085
HapII	CCGG	1 cut(s)	111
HgaI	GACGC	1 cut(s)	1292
HhaI	GCGC	1 cut(s)	891
Hin6I	GCGC	1 cut(s)	889
HinP1I	GCGC	1 cut(s)	889
HincII	GTYRAC	1 cut(s)	1642
HindII	GTYRAC	1 cut(s)	1642
HinfI	GANTC	7 cut(s)	247, 274, 436, 481, 1110, 1153, 1335
HpaI	GTTAAC	1 cut(s)	1642
HpaII	CCGG	1 cut(s)	111
HphI	GGTGA	2 cut(s)	274, 994
Hpy166II	GTNNAC	6 cut(s)	678, 708, 1017, 1287, 1627, 1642
Hpy188I	TCNGA	6 cut(s)	246, 486, 1104, 1345, 1557, 1653
Hpy188III	TCNNGA	8 cut(s)	227, 251, 306, 365, 878, 1181, 1202, 1547
Hpy8I	GTNNAC	6 cut(s)	678, 708, 1017, 1287, 1627, 1642
HpyAV	CCTTC	1 cut(s)	862
HpyCH4III	ACNGT	3 cut(s)	163, 176, 1544
HpyF10VI	GCNNNNNNNGC	4 cut(s)	640, 776, 1051, 1598
HpyF3I	CTNAG	8 cut(s)	414, 579, 1124, 1258, 1296, 1323, 1548, 1593
HspAI	GCGC	1 cut(s)	889
Ksp22I	TGATCA	4 cut(s)	94, 241, 253, 880
KspAI	GTTAAC	1 cut(s)	1642
LmnI	GCTCC	5 cut(s)	507, 654, 1057, 1300, 1653
Lsp1109I	GCAGC	2 cut(s)	223, 275
MaeI	CTAG	6 cut(s)	327, 341, 365, 513, 567, 1214
MaeIII	GTNAC	2 cut(s)	176, 1402
MbiI	CCGCTC	1 cut(s)	1060
MboII	GAAGA	9 cut(s)	26, 92, 639, 761, 826, 848, 1358, 1517, 1556
MflI	RGATCY	3 cut(s)	10, 1348, 1619
MhlI	GDGCHC	2 cut(s)	512, 1019
MlsI	TGGCCA	1 cut(s)	779
MluNI	TGGCCA	1 cut(s)	779
MlyI	GAGTC	2 cut(s)	241, 1344
MmeI	TCCRAC	2 cut(s)	458, 772
Mox20I	TGGCCA	1 cut(s)	779
MroXI	GAANNNNTTC	2 cut(s)	924, 1172
MscI	TGGCCA	1 cut(s)	779
MseI	TTAA	8 cut(s)	171, 407, 552, 855, 1443, 1527, 1539, 1641
MslI	CAYNNNNRTG	1 cut(s)	1683
Msp20I	TGGCCA	1 cut(s)	779
MspA1I	CMGCKG	2 cut(s)	583, 1123
MspI	CCGG	1 cut(s)	111
MspR9I	CCNGG	2 cut(s)	111, 1140
MvaI	CCWGG	1 cut(s)	1140
MvnI	CGCG	1 cut(s)	891
MwoI	GCNNNNNNNGC	4 cut(s)	640, 776, 1051, 1598
NciI	CCSGG	1 cut(s)	111
NlaIV	GGNNCC	4 cut(s)	12, 310, 656, 761
NspI	RCATGY	2 cut(s)	1331, 1514
NspV	TTCGAA	1 cut(s)	434
PaeI	GCATGC	1 cut(s)	1331
PaeR7I	CTCGAG	1 cut(s)	1332
PagI	TCATGA	3 cut(s)	226, 250, 877
PceI	AGGCCT	2 cut(s)	663, 1085
PdmI	GAANNNNTTC	2 cut(s)	924, 1172
PfeI	GAWTC	5 cut(s)	274, 436, 481, 1110, 1153
PfoI	TCCNGGA	1 cut(s)	109
PkrI	GCNGC	3 cut(s)	238, 265, 717
PleI	GAGTC	2 cut(s)	241, 1343
PpsI	GAGTC	2 cut(s)	241, 1343
PpuMI	RGGWCCY	2 cut(s)	221, 308
PshBI	ATTAAT	1 cut(s)	1527
Psp5II	RGGWCCY	2 cut(s)	221, 308
Psp6I	CCWGG	1 cut(s)	1138
PspFI	CCCAGC	1 cut(s)	1134
PspGI	CCWGG	1 cut(s)	1138
PspN4I	GGNNCC	4 cut(s)	12, 310, 656, 761
PspPI	GGNCC	4 cut(s)	221, 308, 760, 963
PspPPI	RGGWCCY	2 cut(s)	221, 308
PspXI	VCTCGAGB	1 cut(s)	1332
PsuI	RGATCY	3 cut(s)	10, 1348, 1619
RsaI	GTAC	3 cut(s)	361, 422, 1376
RsaNI	GTAC	3 cut(s)	360, 421, 1375
RseI	CAYNNNNRTG	1 cut(s)	1683
SaqAI	TTAA	8 cut(s)	171, 407, 552, 855, 1443, 1527, 1539, 1641
SatI	GCNGC	3 cut(s)	237, 264, 716
Sau96I	GGNCC	4 cut(s)	221, 308, 760, 963
ScaI	AGTACT	1 cut(s)	361
SchI	GAGTC	2 cut(s)	241, 1344
ScrFI	CCNGG	2 cut(s)	111, 1140
SduI	GDGCHC	2 cut(s)	512, 1019
Sfr274I	CTCGAG	1 cut(s)	1332
SfuI	TTCGAA	1 cut(s)	434
SinI	GGWCC	3 cut(s)	221, 308, 963
SlaI	CTCGAG	1 cut(s)	1332
SmiMI	CAYNNNNRTG	1 cut(s)	1683
SmlI	CTYRAG	2 cut(s)	1004, 1332
SmoI	CTYRAG	2 cut(s)	1004, 1332
SpeI	ACTAGT	1 cut(s)	512
SphI	GCATGC	1 cut(s)	1331
SseBI	AGGCCT	2 cut(s)	663, 1085
SsiI	CCGC	5 cut(s)	583, 715, 1060, 1096, 1121
SspI	AATATT	1 cut(s)	377
SspMI	CTAG	6 cut(s)	327, 341, 365, 513, 567, 1214
StuI	AGGCCT	2 cut(s)	663, 1085
StyD4I	CCNGG	2 cut(s)	109, 1138
StyI	CCWWGG	4 cut(s)	340, 658, 1191, 1614
TaaI	ACNGT	3 cut(s)	163, 176, 1544
TaqI	TCGA	6 cut(s)	158, 191, 434, 521, 799, 1333
TatI	WGTACW	3 cut(s)	359, 420, 1374
TauI	GCSGC	1 cut(s)	718
TfiI	GAWTC	5 cut(s)	274, 436, 481, 1110, 1153
Tru1I	TTAA	8 cut(s)	171, 407, 552, 855, 1443, 1527, 1539, 1641
Tru9I	TTAA	8 cut(s)	171, 407, 552, 855, 1443, 1527, 1539, 1641
TscAI	CASTG	3 cut(s)	168, 1024, 1276
TseI	GCWGC	2 cut(s)	236, 263
TspDTI	ATGAA	2 cut(s)	881, 1469
TspGWI	ACGGA	1 cut(s)	972
TspRI	CASTG	3 cut(s)	168, 1024, 1276
VneI	GTGCAC	1 cut(s)	1015
VpaK11BI	GGWCC	3 cut(s)	221, 308, 963
VspI	ATTAAT	1 cut(s)	1527
XagI	CCTNNNNNAGG	1 cut(s)	1493
XapI	RAATTY	4 cut(s)	667, 920, 1171, 1244
XbaI	TCTAGA	1 cut(s)	364
XceI	RCATGY	2 cut(s)	1331, 1514
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1198
XhoI	CTCGAG	1 cut(s)	1332
XmaJI	CCTAGG	1 cut(s)	340
XmiI	GTMKAC	2 cut(s)	707, 1286
XmnI	GAANNNNTTC	2 cut(s)	924, 1172
XspI	CTAG	6 cut(s)	327, 341, 365, 513, 567, 1214
ZrmI	AGTACT	1 cut(s)	361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.