MD05G1139000.v1.1

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002573
Physical Location & Seq
Reverse (-)
26956506 .. 26957060
555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1139000.v1.1.491

Sequence Viewer

Length: 555 bp
ATGCTAATTGCAAATGCTGGAGATTGCCGAGCTGTGCTGGGAAGAAGAGGTAGGGCAATTGAGCTTTCCAAAGACCATAAACCCAATTGTGCCTCCGAAAGAGTGAGAATCGAGAAACTGGGAGGCATGGTGTATGATGGCTACCTCAACGGTCAATTATCTGTGGCACGAGCTCTTGGGGACTGGCACATGAAAGGCTCCAAAGGTTCCTTGTGTCCCTTGAGTGCAGAGCCAGAGCTCGAGGAGACAATTCTGAGCGAGGAAGACGAATTCTTAATCCTAGGCTGTGATGGGTTGTGGGATGTGATGAGCAGCCAGTGTGCAGTCACGATGGCAAGGAAAGAACTAATGATCCACAATGATCCCGAGAGATGCTCAAGAGAGCTGGTTCGGGAGGCACTCAAGCGCAATACGTGCGATAACCTGACGGTGGTAGTGGTATGCTTCTCGCCAGATCCACCCCCGTTGCTTGAGATCCCAAAGTTAAAATTCAAGAGGAGTATATCGGCGGAAGGCCTGAATCTTCTGCAGGAGGTACTAGATAGTAAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

185

Amino Acids

20.37

Weight (kDa)

5.5

Isoelectric Point (pI)

54.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 2 - 141 1.4e-52 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 509
AclWI GGATC 4 cut(s) 346, 356, 449, 469
AcsI RAATTY 2 cut(s) 269, 488
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 1 cut(s) 493
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 5 cut(s) 32, 64, 173, 238, 385
AluI AGCT 5 cut(s) 32, 64, 173, 238, 385
Alw21I GWGCWC 2 cut(s) 175, 240
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 4 cut(s) 346, 356, 449, 469
Ama87I CYCGRG 2 cut(s) 239, 365
AoxI GGCC 1 cut(s) 514
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 2 cut(s) 269, 488
AspA2I CCTAGG 1 cut(s) 280
AspLEI GCGC 1 cut(s) 408
AvaI CYCGRG 2 cut(s) 239, 365
AvrII CCTAGG 1 cut(s) 280
BanII GRGCYC 2 cut(s) 175, 240
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 2 cut(s) 175, 240
BbvI GCAGC 1 cut(s) 324
BccI CCATC 3 cut(s) 131, 284, 325
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 281, 539
BfmI CTRYAG 1 cut(s) 527
BisI GCNGC 1 cut(s) 313
BlnI CCTAGG 1 cut(s) 280
BlsI GCNGC 1 cut(s) 314
BmeT110I CYCGRG 2 cut(s) 239, 365
BmiI GGNNCC 2 cut(s) 199, 208
BmrI ACTGGG 1 cut(s) 128
BmsI GCATC 1 cut(s) 362
BmuI ACTGGG 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 359, 391
BpmI CTGGAG 1 cut(s) 39
BpuEI CTTGAG 4 cut(s) 241, 361, 386, 491
BsaAI YACGTR 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 280
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 3 cut(s) 123, 188, 316
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 245
BseNI ACTGG 3 cut(s) 123, 188, 316
BseRI GAGGAG 2 cut(s) 257, 511
BseXI GCAGC 1 cut(s) 324
BseYI CCCAGC 1 cut(s) 37
BsgI GTGCAG 2 cut(s) 246, 342
BshFI GGCC 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 175, 240
BsiHKCI CYCGRG 2 cut(s) 239, 365
BslFI GGGAC 2 cut(s) 194, 201
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 2 cut(s) 194, 201
BsnI GGCC 1 cut(s) 516
BsoBI CYCGRG 2 cut(s) 239, 365
Bsp1286I GDGCHC 2 cut(s) 175, 240
Bsp143I GATC 4 cut(s) 351, 361, 454, 474
BspACI CCGC 1 cut(s) 509
BspANI GGCC 1 cut(s) 516
BspCNI CTCAG 1 cut(s) 246
BspHI TCATGA 1 cut(s) 551
BspLI GGNNCC 2 cut(s) 199, 208
BspMAI CTGCAG 1 cut(s) 531
BspPI GGATC 4 cut(s) 346, 356, 449, 469
BsrI ACTGG 3 cut(s) 123, 188, 316
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 4 cut(s) 351, 361, 454, 474
BssSI CACGAG 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 280
Bst2BI CACGAG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 152, 430
Bst6I CTCTTC 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 414
BstDEI CTNAG 1 cut(s) 254
BstF5I GGATG 1 cut(s) 307
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 4 cut(s) 354, 364, 457, 477
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 4 cut(s) 351, 361, 454, 474
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstSFI CTRYAG 1 cut(s) 527
BstV1I GCAGC 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 2 cut(s) 454, 474
BstYI RGATCY 2 cut(s) 454, 474
BsuRI GGCC 1 cut(s) 516
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 323
CciI TCATGA 1 cut(s) 551
CfoI GCGC 1 cut(s) 408
Csp6I GTAC 1 cut(s) 536
CspCI CAANNNNNGTGG 2 cut(s) 447, 482
CviAII CATG 3 cut(s) 127, 190, 552
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 1 cut(s) 254
DpnI GATC 4 cut(s) 353, 363, 456, 476
DpnII GATC 4 cut(s) 351, 361, 454, 474
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
EciI GGCGGA 1 cut(s) 524
Ecl136II GAGCTC 2 cut(s) 173, 238
Eco130I CCWWGG 1 cut(s) 280
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 2 cut(s) 175, 240
Eco53kI GAGCTC 2 cut(s) 173, 238
Eco88I CYCGRG 2 cut(s) 239, 365
EcoICRI GAGCTC 2 cut(s) 173, 238
EcoRI GAATTC 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 280
EcoT38I GRGCYC 2 cut(s) 175, 240
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 3 cut(s) 130, 193, 555
FaiI YATR 7 cut(s) 78, 128, 135, 191, 442, 503, 553
FaqI GGGAC 2 cut(s) 194, 201
FatI CATG 3 cut(s) 126, 189, 551
Fnu4HI GCNGC 1 cut(s) 313
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 2 cut(s) 175, 240
Fsp4HI GCNGC 1 cut(s) 313
FspBI CTAG 2 cut(s) 281, 539
GlaI GCGC 1 cut(s) 407
GluI GCNGC 1 cut(s) 313
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 1 cut(s) 516
HhaI GCGC 1 cut(s) 408
Hin1II CATG 3 cut(s) 130, 193, 555
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HinfI GANTC 2 cut(s) 108, 520
Hpy188I TCNGA 2 cut(s) 97, 255
Hpy188III TCNNGA 7 cut(s) 112, 328, 365, 378, 392, 493, 552
HpyAV CCTTC 1 cut(s) 506
HpyCH4III ACNGT 2 cut(s) 152, 430
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 4 cut(s) 11, 227, 323, 529
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 3 cut(s) 130, 193, 555
HspAI GCGC 1 cut(s) 406
Kzo9I GATC 4 cut(s) 351, 361, 454, 474
LmnI GCTCC 1 cut(s) 203
Lsp1109I GCAGC 1 cut(s) 324
LweI GCATC 1 cut(s) 362
MaeI CTAG 2 cut(s) 281, 539
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 325
MalI GATC 4 cut(s) 353, 363, 456, 476
MboI GATC 4 cut(s) 351, 361, 454, 474
MboII GAAGA 4 cut(s) 54, 57, 275, 515
MfeI CAATTG 2 cut(s) 57, 85
MflI RGATCY 2 cut(s) 454, 474
MhlI GDGCHC 2 cut(s) 175, 240
MluCI AATT 7 cut(s) 6, 57, 85, 155, 249, 269, 488
MnlI CCTC 9 cut(s) 41, 103, 116, 155, 235, 253, 388, 489, 526
MseI TTAA 2 cut(s) 275, 485
MunI CAATTG 2 cut(s) 57, 85
MwoI GCNNNNNNNGC 1 cut(s) 414
NdeII GATC 4 cut(s) 351, 361, 454, 474
NlaIII CATG 3 cut(s) 130, 193, 555
NlaIV GGNNCC 2 cut(s) 199, 208
NmeAIII GCCGAG 1 cut(s) 53
NmuCI GTSAC 1 cut(s) 325
PaeR7I CTCGAG 1 cut(s) 239
PagI TCATGA 1 cut(s) 551
PceI AGGCCT 1 cut(s) 516
PfeI GAWTC 2 cut(s) 108, 520
PkrI GCNGC 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 414
Psp124BI GAGCTC 2 cut(s) 175, 240
PspFI CCCAGC 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 199, 208
PspXI VCTCGAGB 1 cut(s) 239
PstI CTGCAG 1 cut(s) 531
PsuI RGATCY 2 cut(s) 454, 474
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SacI GAGCTC 2 cut(s) 175, 240
SaqAI TTAA 2 cut(s) 275, 485
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 4 cut(s) 351, 361, 454, 474
SduI GDGCHC 2 cut(s) 175, 240
SfaNI GCATC 1 cut(s) 362
SfcI CTRYAG 1 cut(s) 527
Sfr274I CTCGAG 1 cut(s) 239
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 5 cut(s) 220, 239, 376, 401, 470
SmoI CTYRAG 5 cut(s) 220, 239, 376, 401, 470
Sse9I AATT 7 cut(s) 6, 57, 85, 155, 249, 269, 488
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 1 cut(s) 509
SspMI CTAG 2 cut(s) 281, 539
SstI GAGCTC 2 cut(s) 175, 240
StuI AGGCCT 1 cut(s) 516
StyI CCWWGG 1 cut(s) 280
TaaI ACNGT 2 cut(s) 152, 430
TaiI ACGT 1 cut(s) 416
TaqI TCGA 2 cut(s) 111, 240
TasI AATT 7 cut(s) 6, 57, 85, 155, 249, 269, 488
TfiI GAWTC 2 cut(s) 108, 520
Tru1I TTAA 2 cut(s) 275, 485
Tru9I TTAA 2 cut(s) 275, 485
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 206
TspRI CASTG 1 cut(s) 323
XapI RAATTY 2 cut(s) 269, 488
XhoI CTCGAG 1 cut(s) 239
XmaJI CCTAGG 1 cut(s) 280
XspI CTAG 2 cut(s) 281, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.