Rh6BG251000

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002573
Physical Location & Seq
Reverse (-)
46271966 .. 46274038
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG251000.1

Sequence Viewer

Length: 1158 bp
ATGGCTGCAGGGACAGATACCCCATCTTCACTTTCCATGTTAGATGGCAGTTACAGAAAGAACGTAACTGTTACAAAGGACGAGAAATCAAATACTTTTGAGAACATGAAGCAATCCAACATCGGCAAACCTCCTAGGCATCTTTCAGCTATTAGGAACTGTGTCAGCTCTGCCCAGTTGGCTCCTGCAACTGAAGTGGAGTTCGATAGTGGAATTGTTAGCTCAAAATCCTCGTCAGATAGTAATTCACCATTTGTACCTGCTTTTCGGTCTGGAAGCTGTTCAGAGATTGGATCAAAACAATTTATGGAGGATGAGCATATTTGTATAGACAATATTCTTGAACATCTAGGATCTACTGCAGACTTTCCTTCTCCTGGAGCTTTCTATGGGGTTTTTGATGGCCATGGTGGTACAGATGCAGCTTCTTATATCAGAGAGAATATTTTGAGGTTTATAGTGGAGGATTCACATTTCCCTTTTTGTGCTAAGCAAGCTATTAAGAGTGCTTTTCTAAAGGCTGACCATGCTTTTGCTGATAATGGTACTCTTGATAGTTCCTCTGGTACCACTGCACTTACTGCCCTTATCTCTGGAAGGACGATGCTGGTTGCCAATGCTGGAGATTGTCGAGCTGTTCTGGGAAGAAGAGGCAGGGCAGTGGAACTTTCCAAAGACCATAAACCAAATTGTGCTTCAGAAAGAGTAAGAATTGAAGAACTGGGAGGCATGGTGTATGATGGATACCTCAACGGCCAATTGTCAGTGGCGCGAGCACTTGGAGATTGGCACATGAAAGGTTCCAAAGGTTCCTCTTGTCCCTTAAGTGCAGAACCAGAGCTTGAGGAGACAATTCTGGGCGAGGAAGATGAGTTCTTGATCATAGGCTGTGATGGGCTGTGGGATGTGATGAGCAGCCAGTGTGCCGTCACAATTGCAAGGAAAGAATTGATGCTTCACAATGATCCTGAAAGATGCTCCAGAGAGCTAGTTAGGGAAGCTCTCAAGCGCGATACACGTGATAACTTGACCGTTGTAGTGGTATGCTTCTCCCCAGAACCACCGCCTAAGATTGAGATCCCAAAGTTCAAGTTCAAGAGGAGTATATCAGCAGAAGGTCTGAATCTTCTGCAGGAAGTACTAGATAGTAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

385

Amino Acids

41.77

Weight (kDa)

5.25

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 92 - 342 3.7e-75 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
Acc65I GGTACC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 566
AccII CGCG 2 cut(s) 772, 1011
AciI CCGC 1 cut(s) 1064
AclWI GGATC 4 cut(s) 301, 361, 959, 1072
AcoI YGGCCR 2 cut(s) 403, 754
AcuI CTGAAG 2 cut(s) 213, 681
AcvI CACGTG 1 cut(s) 1019
AfaI GTAC 5 cut(s) 258, 415, 547, 568, 1140
AfiI CCNNNNNNNGG 1 cut(s) 377
AflII CTTAAG 1 cut(s) 823
AflIII ACRYGT 1 cut(s) 1016
AgsI TTSAA 4 cut(s) 344, 716, 1090, 1096
AjnI CCWGG 1 cut(s) 376
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
AloI GAACNNNNNNTCC 2 cut(s) 857, 889
Alw21I GWGCWC 1 cut(s) 778
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 4 cut(s) 301, 361, 959, 1072
AoxI GGCC 2 cut(s) 403, 754
ApeKI GCWGC 3 cut(s) 5, 422, 915
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 515, 547
Asp700I GAANNNNTTC 1 cut(s) 280
Asp718I GGTACC 1 cut(s) 566
AspA2I CCTAGG 1 cut(s) 134
AspLEI GCGC 2 cut(s) 772, 1011
AsuHPI GGTGA 1 cut(s) 240
AvrII CCTAGG 1 cut(s) 134
BalI TGGCCA 1 cut(s) 405
BanI GGYRCC 1 cut(s) 566
BbrPI CACGTG 1 cut(s) 1019
Bbv12I GWGCWC 1 cut(s) 778
BbvI GCAGC 2 cut(s) 434, 927
BccI CCATC 5 cut(s) 31, 38, 395, 734, 887
BceAI ACGGC 2 cut(s) 769, 911
BciT130I CCWGG 1 cut(s) 378
BciVI GTATCC 1 cut(s) 737
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 1 cut(s) 842
BfaI CTAG 4 cut(s) 135, 350, 989, 1142
BfmI CTRYAG 3 cut(s) 6, 360, 1130
BfrI CTTAAG 1 cut(s) 823
BfuAI ACCTGC 1 cut(s) 268
BfuI GTATCC 1 cut(s) 737
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 3 cut(s) 6, 423, 916
BlnI CCTAGG 1 cut(s) 134
BlpI GCTNAGC 1 cut(s) 489
BlsI GCNGC 3 cut(s) 7, 424, 917
BmcAI AGTACT 1 cut(s) 1140
Bme1390I CCNGG 1 cut(s) 378
BmiI GGNNCC 4 cut(s) 183, 568, 802, 811
BmrFI CCNGG 1 cut(s) 378
BmrI ACTGGG 2 cut(s) 169, 731
BmsI GCATC 5 cut(s) 148, 409, 594, 942, 965
BmuI ACTGGG 2 cut(s) 169, 731
BpmI CTGGAG 3 cut(s) 399, 642, 964
Bpu1102I GCTNAGC 1 cut(s) 489
BpuEI CTTGAG 2 cut(s) 863, 989
BsaAI YACGTR 1 cut(s) 1019
BsaBI GATNNNNATC 1 cut(s) 1076
BsaJI CCNNGG 2 cut(s) 134, 406
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 3 cut(s) 175, 726, 919
Bse8I GATNNNNATC 1 cut(s) 1076
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 2 cut(s) 134, 406
BseGI GGATG 2 cut(s) 319, 910
BseJI GATNNNNATC 1 cut(s) 1076
BseLI CCNNNNNNNGG 1 cut(s) 377
BseNI ACTGG 3 cut(s) 175, 726, 919
BseRI GAGGAG 2 cut(s) 860, 1114
BseXI GCAGC 2 cut(s) 434, 927
BsgI GTGCAG 2 cut(s) 558, 849
Bsh1236I CGCG 2 cut(s) 772, 1011
BshFI GGCC 2 cut(s) 405, 756
BshNI GGYRCC 1 cut(s) 566
BsiHKAI GWGCWC 1 cut(s) 778
BslFI GGGAC 2 cut(s) 25, 804
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 842
BsmFI GGGAC 2 cut(s) 25, 804
BsnI GGCC 2 cut(s) 405, 756
Bsp1286I GDGCHC 1 cut(s) 778
Bsp143I GATC 5 cut(s) 293, 353, 879, 964, 1077
Bsp1720I GCTNAGC 1 cut(s) 489
Bsp19I CCATGG 1 cut(s) 406
BspACI CCGC 1 cut(s) 1064
BspANI GGCC 2 cut(s) 405, 756
BspFNI CGCG 2 cut(s) 772, 1011
BspLI GGNNCC 4 cut(s) 183, 568, 802, 811
BspMAI CTGCAG 3 cut(s) 10, 364, 1134
BspMI ACCTGC 1 cut(s) 268
BspPI GGATC 4 cut(s) 301, 361, 959, 1072
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 1 cut(s) 823
BsrI ACTGG 3 cut(s) 175, 726, 919
BssECI CCNNGG 2 cut(s) 134, 406
BssMI GATC 5 cut(s) 293, 353, 879, 964, 1077
BssT1I CCWWGG 2 cut(s) 134, 406
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 3 cut(s) 70, 161, 1033
Bst6I CTCTTC 1 cut(s) 643
BstAFI CTTAAG 1 cut(s) 823
BstAPI GCANNNNNTGC 1 cut(s) 581
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 2 cut(s) 495, 774
BstDEI CTNAG 2 cut(s) 489, 1068
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 2 cut(s) 319, 910
BstFNI CGCG 2 cut(s) 772, 1011
BstHHI GCGC 2 cut(s) 772, 1011
BstKTI GATC 5 cut(s) 296, 356, 882, 967, 1080
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 5 cut(s) 293, 353, 879, 964, 1077
BstMWI GCNNNNNNNGC 4 cut(s) 179, 494, 527, 581
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 376
BstSFI CTRYAG 3 cut(s) 6, 360, 1130
BstUI CGCG 2 cut(s) 772, 1011
BstV1I GCAGC 2 cut(s) 434, 927
BstX2I RGATCY 2 cut(s) 353, 1077
BstYI RGATCY 2 cut(s) 353, 1077
BsuI GTATCC 1 cut(s) 737
BsuRI GGCC 2 cut(s) 405, 756
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 2 cut(s) 319, 910
BtsI GCAGTG 2 cut(s) 570, 666
BtsIMutI CAGTG 4 cut(s) 570, 666, 771, 926
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 2 cut(s) 495, 774
CfoI GCGC 2 cut(s) 772, 1011
Csp6I GTAC 5 cut(s) 257, 414, 546, 567, 1139
CspCI CAANNNNNGTGG 2 cut(s) 177, 212
CviAII CATG 6 cut(s) 37, 106, 407, 527, 730, 793
CviQI GTAC 5 cut(s) 257, 414, 546, 567, 1139
DdeI CTNAG 2 cut(s) 489, 1068
DpnI GATC 5 cut(s) 295, 355, 881, 966, 1079
DpnII GATC 5 cut(s) 293, 353, 879, 964, 1077
EaeI YGGCCR 2 cut(s) 403, 754
Eam1104I CTCTTC 1 cut(s) 643
EarI CTCTTC 1 cut(s) 643
Eco130I CCWWGG 2 cut(s) 134, 406
Eco57I CTGAAG 2 cut(s) 213, 681
Eco72I CACGTG 1 cut(s) 1019
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 134, 406
ErhI CCWWGG 2 cut(s) 134, 406
FaeI CATG 6 cut(s) 40, 109, 410, 530, 733, 796
FaqI GGGAC 2 cut(s) 25, 804
FatI CATG 6 cut(s) 36, 105, 406, 526, 729, 792
FbaI TGATCA 1 cut(s) 879
Fnu4HI GCNGC 3 cut(s) 6, 423, 916
FokI GGATG 2 cut(s) 326, 917
Fsp4HI GCNGC 3 cut(s) 6, 423, 916
FspBI CTAG 4 cut(s) 135, 350, 989, 1142
GlaI GCGC 2 cut(s) 771, 1010
GluI GCNGC 3 cut(s) 6, 423, 916
GsuI CTGGAG 3 cut(s) 399, 642, 964
HaeIII GGCC 2 cut(s) 405, 756
HhaI GCGC 2 cut(s) 772, 1011
Hin1II CATG 6 cut(s) 40, 109, 410, 530, 733, 796
Hin6I GCGC 2 cut(s) 770, 1009
HinP1I GCGC 2 cut(s) 770, 1009
HinfI GANTC 2 cut(s) 467, 1123
HphI GGTGA 1 cut(s) 240
Hpy188I TCNGA 5 cut(s) 238, 286, 437, 700, 1122
Hpy188III TCNNGA 8 cut(s) 273, 341, 551, 594, 877, 968, 981, 1096
HpyAV CCTTC 3 cut(s) 381, 591, 1109
HpyCH4III ACNGT 3 cut(s) 70, 161, 1033
HpyCH4IV ACGT 2 cut(s) 63, 1018
HpyCH4V TGCA 8 cut(s) 8, 188, 362, 422, 575, 830, 938, 1132
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 494, 527, 581
HpyF3I CTNAG 2 cut(s) 489, 1068
HpySE526I ACGT 2 cut(s) 63, 1018
Hsp92II CATG 6 cut(s) 40, 109, 410, 530, 733, 796
HspAI GCGC 2 cut(s) 770, 1009
KpnI GGTACC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 5 cut(s) 293, 353, 879, 964, 1077
LmnI GCTCC 3 cut(s) 187, 380, 983
Lsp1109I GCAGC 2 cut(s) 434, 927
LweI GCATC 5 cut(s) 148, 409, 594, 942, 965
MaeI CTAG 4 cut(s) 135, 350, 989, 1142
MaeII ACGT 2 cut(s) 63, 1018
MaeIII GTNAC 4 cut(s) 50, 64, 70, 928
MalI GATC 5 cut(s) 295, 355, 881, 966, 1079
MboI GATC 5 cut(s) 293, 353, 879, 964, 1077
MboII GAAGA 6 cut(s) 18, 657, 660, 728, 878, 1118
MfeI CAATTG 2 cut(s) 758, 933
MflI RGATCY 2 cut(s) 353, 1077
MhlI GDGCHC 1 cut(s) 778
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 9 cut(s) 213, 244, 302, 688, 711, 758, 852, 933, 947
MluNI TGGCCA 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 141
Mox20I TGGCCA 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 280
MscI TGGCCA 1 cut(s) 405
MseI TTAA 2 cut(s) 501, 824
Msp20I TGGCCA 1 cut(s) 405
MspCI CTTAAG 1 cut(s) 823
MspR9I CCNGG 1 cut(s) 378
MunI CAATTG 2 cut(s) 758, 933
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 2 cut(s) 772, 1011
MwoI GCNNNNNNNGC 4 cut(s) 179, 494, 527, 581
NcoI CCATGG 1 cut(s) 406
NdeII GATC 5 cut(s) 293, 353, 879, 964, 1077
NlaIII CATG 6 cut(s) 40, 109, 410, 530, 733, 796
NlaIV GGNNCC 4 cut(s) 183, 568, 802, 811
NmuCI GTSAC 1 cut(s) 928
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 2 cut(s) 467, 1123
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 3 cut(s) 7, 424, 917
PmaCI CACGTG 1 cut(s) 1019
PmlI CACGTG 1 cut(s) 1019
Ppu21I YACGTR 1 cut(s) 1019
Psp6I CCWGG 1 cut(s) 376
PspCI CACGTG 1 cut(s) 1019
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 4 cut(s) 183, 568, 802, 811
PstI CTGCAG 3 cut(s) 10, 364, 1134
PsuI RGATCY 2 cut(s) 353, 1077
RsaI GTAC 5 cut(s) 258, 415, 547, 568, 1140
RsaNI GTAC 5 cut(s) 257, 414, 546, 567, 1139
SaqAI TTAA 2 cut(s) 501, 824
SatI GCNGC 3 cut(s) 6, 423, 916
Sau3AI GATC 5 cut(s) 293, 353, 879, 964, 1077
ScaI AGTACT 1 cut(s) 1140
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 778
SfaNI GCATC 5 cut(s) 148, 409, 594, 942, 965
SfcI CTRYAG 3 cut(s) 6, 360, 1130
SmlI CTYRAG 3 cut(s) 823, 842, 1004
SmoI CTYRAG 3 cut(s) 823, 842, 1004
Sse9I AATT 9 cut(s) 213, 244, 302, 688, 711, 758, 852, 933, 947
SsiI CCGC 1 cut(s) 1064
SspI AATATT 2 cut(s) 337, 445
SspMI CTAG 4 cut(s) 135, 350, 989, 1142
StyD4I CCNGG 1 cut(s) 376
StyI CCWWGG 2 cut(s) 134, 406
TaaI ACNGT 3 cut(s) 70, 161, 1033
TaiI ACGT 2 cut(s) 66, 1021
TaqI TCGA 2 cut(s) 204, 631
TaqII GACCGA 1 cut(s) 258
TasI AATT 9 cut(s) 213, 244, 302, 688, 711, 758, 852, 933, 947
TatI WGTACW 1 cut(s) 1138
TfiI GAWTC 2 cut(s) 467, 1123
Tru1I TTAA 2 cut(s) 501, 824
Tru9I TTAA 2 cut(s) 501, 824
TscAI CASTG 4 cut(s) 577, 666, 771, 926
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 3 cut(s) 5, 422, 915
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 2 cut(s) 122, 809
TspRI CASTG 4 cut(s) 577, 666, 771, 926
Vha464I CTTAAG 1 cut(s) 823
XmaJI CCTAGG 1 cut(s) 134
XmnI GAANNNNTTC 1 cut(s) 280
XspI CTAG 4 cut(s) 135, 350, 989, 1142
ZrmI AGTACT 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.