Rorug06G0136500

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002573
Physical Location & Seq
Reverse (-)
19285128 .. 19286824
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0136500.1

Sequence Viewer

Length: 1026 bp
ATGCTCAGAATCTTCACAGGCAAGTCCATAAGAAACCAAAACTACCCACCTGTAAAAGGCACCATCTTTAATCACCTCTTATACTACAACAAACTATATGACTATCACATTCACACCCGAGTTGCGAGAGAATGCCCACCTCACCGGCTTCTTGCTCTAAACAGCAGCACAGTATACACATGTGACCCTCGAAACATTGAGCATTTTCTCAAGACCAACTTTGCTCATTACTCGAAAAGTGACTATAACCAATGTATTATGTCTGAGATTTTGGGACAGGGCATTTTTGTTGTTAATGGAGAGAAATTGAGGCAGCAGAGGAAGCTTGCTAGCTTTGAGTTCTCAACAAGGAATCTTGTTAGAGTTGTTTTTGAGTTTTTGGATTCCAAAAGGGTCTTTGATATGCAGTATGTCAAAGTCACCCATGATTTTGTGCACCAACTTATCAGGAGAAAGAGGGAACTTCTAGCTGGCAAGAAAGATGGTGTAAGTGACAAGGAGGACATACTATCAAGGTTTATGGAAAGTGAGAAGGATCCAGAGCAAAAGAATGACAAATATCCAGGTGATATAATTCTGAATTTTATGATAGCTGGCAAAGATACAAGTGCAAATACACTGTCATGGTTCTTCTACATGCTCAGCAAGAACCCTCTAATACAGGAAAAAGTTGCACAAGAAGTGAAGGATGTTGTCTGTATGAAACACGACGCTGACATTGATGAATTTGTGACGAACATAACTGATGCAACTCTTGAAAAGATGCAGTATCTTCATGCCACACTAACAGAGACCTTGAGGCTCTACCCTGCAGTTCCTGTGGCAGGACCTCGGACCTGTCTAGGGAAGGACTTGGCTTACAGGCAGATTAAGATAGTAGCAATGGCTCTTCTTTGCTTCTTCCGCTTCAAATTGGCTGATGAAACAAGAAATGTAACCTATGGGACCATGTTCACCCTTCACATAGATGGCACCCTCCCTATGCTTGTACTTCCAAGGACCACCTCACAGATTATTTTCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

341

Amino Acids

39.66

Weight (kDa)

9.03

Isoelectric Point (pI)

37.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 131 - 273 3.8e-24 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 59, 971
AccI GTMKAC 1 cut(s) 174
AciI CCGC 1 cut(s) 904
AclWI GGATC 2 cut(s) 530, 543
AcsI RAATTY 2 cut(s) 580, 725
AfaI GTAC 1 cut(s) 990
AfiI CCNNNNNNNGG 3 cut(s) 56, 824, 843
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 2 cut(s) 758, 910
AjnI CCWGG 1 cut(s) 562
AluBI AGCT 4 cut(s) 325, 333, 470, 593
AluI AGCT 4 cut(s) 325, 333, 470, 593
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 1 cut(s) 785
Alw44I GTGCAC 1 cut(s) 434
AlwI GGATC 2 cut(s) 530, 543
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 117
ApaLI GTGCAC 1 cut(s) 434
ApeKI GCWGC 2 cut(s) 165, 313
ApoI RAATTY 2 cut(s) 580, 725
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
AspS9I GGNCC 4 cut(s) 827, 834, 945, 999
AsuHPI GGTGA 5 cut(s) 65, 134, 412, 578, 946
AsuNHI GCTAGC 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 4 cut(s) 827, 834, 945, 999
BaeGI GKGCMC 1 cut(s) 438
BamHI GGATCC 1 cut(s) 535
BanI GGYRCC 2 cut(s) 59, 971
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 177, 325
BccI CCATC 3 cut(s) 71, 476, 962
BciT130I CCWGG 1 cut(s) 564
BcoDI GTCTC 1 cut(s) 785
BfaI CTAG 3 cut(s) 330, 467, 842
BfmI CTRYAG 1 cut(s) 810
BisI GCNGC 2 cut(s) 166, 314
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 2 cut(s) 167, 315
Bme1390I CCNGG 1 cut(s) 564
Bme18I GGWCC 4 cut(s) 827, 834, 945, 999
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 4 cut(s) 827, 834, 945, 999
BmiI GGNNCC 4 cut(s) 61, 537, 946, 973
BmrFI CCNGG 1 cut(s) 564
BmsI GCATC 2 cut(s) 736, 753
BmtI GCTAGC 1 cut(s) 333
Bpu1102I GCTNAGC 1 cut(s) 641
BpuEI CTTGAG 2 cut(s) 194, 817
BsaI GGTCTC 1 cut(s) 785
BsaJI CCNNGG 2 cut(s) 830, 995
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 824, 843
Bse118I RCCGGY 1 cut(s) 144
Bse3DI GCAATG 1 cut(s) 888
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 830, 995
BseGI GGATG 1 cut(s) 694
BseLI CCNNNNNNNGG 3 cut(s) 56, 824, 843
BseMI GCAATG 1 cut(s) 888
BseMII CTCAG 3 cut(s) 19, 255, 655
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 2 cut(s) 177, 325
BshNI GGYRCC 2 cut(s) 59, 971
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 1 cut(s) 145
BslFI GGGAC 2 cut(s) 288, 958
BslI CCNNNNNNNGG 3 cut(s) 56, 824, 843
BsmAI GTCTC 1 cut(s) 785
BsmFI GGGAC 2 cut(s) 288, 958
BsmI GAATGC 1 cut(s) 137
Bso31I GGTCTC 1 cut(s) 785
BsoBI CYCGRG 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 1 cut(s) 535
Bsp1720I GCTNAGC 1 cut(s) 641
BspACI CCGC 1 cut(s) 904
BspCNI CTCAG 3 cut(s) 18, 256, 654
BspLI GGNNCC 4 cut(s) 61, 537, 946, 973
BspMAI CTGCAG 1 cut(s) 814
BspOI GCTAGC 1 cut(s) 333
BspPI GGATC 2 cut(s) 530, 543
BspQI GCTCTTC 1 cut(s) 894
BspT107I GGYRCC 2 cut(s) 59, 971
BspTNI GGTCTC 1 cut(s) 785
BsrDI GCAATG 1 cut(s) 888
BsrFI RCCGGY 1 cut(s) 144
BssAI RCCGGY 1 cut(s) 144
BssECI CCNNGG 2 cut(s) 830, 995
BssMI GATC 1 cut(s) 535
BssNAI GTATAC 1 cut(s) 175
BssT1I CCWWGG 1 cut(s) 995
Bst1107I GTATAC 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 2 cut(s) 172, 621
Bst6I CTCTTC 1 cut(s) 894
BstC8I GCNNGC 4 cut(s) 327, 331, 472, 595
BstDEI CTNAG 3 cut(s) 5, 264, 641
BstENI CCTNNNNNAGG 2 cut(s) 54, 822
BstF5I GGATG 1 cut(s) 694
BstKTI GATC 1 cut(s) 538
BstMAI GTCTC 1 cut(s) 785
BstMBI GATC 1 cut(s) 535
BstMWI GCNNNNNNNGC 2 cut(s) 322, 903
BstNI CCWGG 1 cut(s) 564
BstNSI RCATGY 2 cut(s) 183, 640
BstSCI CCNGG 1 cut(s) 562
BstSFI CTRYAG 1 cut(s) 810
BstSLI GKGCMC 1 cut(s) 438
BstV1I GCAGC 2 cut(s) 177, 325
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BstZ17I GTATAC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 694
BtsIMutI CAGTG 1 cut(s) 617
Cac8I GCNNGC 4 cut(s) 327, 331, 472, 595
CaiI CAGNNNCTG 1 cut(s) 818
Cfr10I RCCGGY 1 cut(s) 144
Cfr13I GGNCC 4 cut(s) 827, 834, 945, 999
CseI GACGC 1 cut(s) 719
Csp6I GTAC 1 cut(s) 989
CviAII CATG 6 cut(s) 180, 425, 624, 637, 776, 949
CviJI RGCY 9 cut(s) 148, 325, 333, 470, 593, 802, 857, 887, 917
CviKI_1 RGCY 9 cut(s) 148, 325, 333, 470, 593, 802, 857, 887, 917
CviQI GTAC 1 cut(s) 989
DdeI CTNAG 3 cut(s) 5, 264, 641
DpnI GATC 1 cut(s) 537
DpnII GATC 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Eco130I CCWWGG 1 cut(s) 995
Eco31I GGTCTC 1 cut(s) 785
Eco47I GGWCC 4 cut(s) 827, 834, 945, 999
Eco88I CYCGRG 1 cut(s) 117
EcoNI CCTNNNNNAGG 2 cut(s) 54, 822
EcoO109I RGGNCCY 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 995
ErhI CCWWGG 1 cut(s) 995
FaeI CATG 6 cut(s) 183, 428, 627, 640, 779, 952
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 2 cut(s) 288, 958
FatI CATG 6 cut(s) 179, 424, 623, 636, 775, 948
FauNDI CATATG 1 cut(s) 1022
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 166, 314
FokI GGATG 1 cut(s) 701
Fsp4HI GCNGC 2 cut(s) 166, 314
FspBI CTAG 3 cut(s) 330, 467, 842
GluI GCNGC 2 cut(s) 166, 314
HapII CCGG 1 cut(s) 145
HgaI GACGC 1 cut(s) 719
Hin1II CATG 6 cut(s) 183, 428, 627, 640, 779, 952
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 3 cut(s) 9, 352, 383
HpaII CCGG 1 cut(s) 145
HphI GGTGA 5 cut(s) 65, 134, 412, 578, 946
Hpy166II GTNNAC 3 cut(s) 175, 436, 954
Hpy188I TCNGA 4 cut(s) 8, 265, 579, 834
Hpy188III TCNNGA 4 cut(s) 211, 448, 539, 755
Hpy8I GTNNAC 3 cut(s) 175, 436, 954
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 4 cut(s) 526, 679, 841, 968
HpyCH4III ACNGT 2 cut(s) 172, 621
HpyCH4V TGCA 7 cut(s) 406, 436, 611, 674, 749, 766, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 322, 903
HpyF3I CTNAG 3 cut(s) 5, 264, 641
Hsp92II CATG 6 cut(s) 183, 428, 627, 640, 779, 952
Kzo9I GATC 1 cut(s) 535
LguI GCTCTTC 1 cut(s) 894
Lsp1109I GCAGC 2 cut(s) 177, 325
LweI GCATC 2 cut(s) 736, 753
MaeI CTAG 3 cut(s) 330, 467, 842
MaeIII GTNAC 6 cut(s) 182, 239, 418, 491, 730, 934
MalI GATC 1 cut(s) 537
MboI GATC 1 cut(s) 535
MboII GAAGA 5 cut(s) 4, 622, 764, 881, 892
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 5 cut(s) 305, 573, 580, 725, 911
MseI TTAA 3 cut(s) 69, 294, 870
MslI CAYNNNNRTG 2 cut(s) 622, 966
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 1 cut(s) 564
Mva1269I GAATGC 1 cut(s) 137
MvaI CCWGG 1 cut(s) 564
MwoI GCNNNNNNNGC 2 cut(s) 322, 903
NdeI CATATG 1 cut(s) 1022
NdeII GATC 1 cut(s) 535
NheI GCTAGC 1 cut(s) 329
NlaIII CATG 6 cut(s) 183, 428, 627, 640, 779, 952
NlaIV GGNNCC 4 cut(s) 61, 537, 946, 973
NmuCI GTSAC 5 cut(s) 182, 239, 418, 491, 730
NspI RCATGY 2 cut(s) 183, 640
PciI ACATGT 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 894
PctI GAATGC 1 cut(s) 137
PfeI GAWTC 3 cut(s) 9, 352, 383
PkrI GCNGC 2 cut(s) 167, 315
PpuMI RGGWCCY 1 cut(s) 827
PscI ACATGT 1 cut(s) 179
Psp5II RGGWCCY 1 cut(s) 827
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 4 cut(s) 61, 537, 946, 973
PspPI GGNCC 4 cut(s) 827, 834, 945, 999
PspPPI RGGWCCY 1 cut(s) 827
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 1 cut(s) 990
RsaNI GTAC 1 cut(s) 989
RseI CAYNNNNRTG 2 cut(s) 622, 966
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 3 cut(s) 69, 294, 870
SatI GCNGC 2 cut(s) 166, 314
Sau3AI GATC 1 cut(s) 535
Sau96I GGNCC 4 cut(s) 827, 834, 945, 999
ScrFI CCNGG 1 cut(s) 564
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 2 cut(s) 736, 753
SfcI CTRYAG 1 cut(s) 810
SinI GGWCC 4 cut(s) 827, 834, 945, 999
SmiMI CAYNNNNRTG 2 cut(s) 622, 966
SmlI CTYRAG 2 cut(s) 209, 796
SmoI CTYRAG 2 cut(s) 209, 796
Sse9I AATT 5 cut(s) 305, 573, 580, 725, 911
SsiI CCGC 1 cut(s) 904
SspMI CTAG 3 cut(s) 330, 467, 842
StyD4I CCNGG 1 cut(s) 562
StyI CCWWGG 1 cut(s) 995
TaaI ACNGT 2 cut(s) 172, 621
TaqI TCGA 2 cut(s) 190, 233
TasI AATT 5 cut(s) 305, 573, 580, 725, 911
TatI WGTACW 1 cut(s) 988
TfiI GAWTC 3 cut(s) 9, 352, 383
Tru1I TTAA 3 cut(s) 69, 294, 870
Tru9I TTAA 3 cut(s) 69, 294, 870
TscAI CASTG 1 cut(s) 624
TseFI GTSAC 5 cut(s) 182, 239, 418, 491, 730
TseI GCWGC 2 cut(s) 165, 313
Tsp45I GTSAC 5 cut(s) 182, 239, 418, 491, 730
TspDTI ATGAA 5 cut(s) 716, 738, 764, 936, 1009
TspRI CASTG 1 cut(s) 624
VneI GTGCAC 1 cut(s) 434
VpaK11BI GGWCC 4 cut(s) 827, 834, 945, 999
XagI CCTNNNNNAGG 2 cut(s) 54, 822
XapI RAATTY 2 cut(s) 580, 725
XceI RCATGY 2 cut(s) 183, 640
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 3 cut(s) 330, 467, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.