pycom10g12160

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002573
Physical Location & Seq
Reverse (-)
15736174 .. 15738155
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g12160.1

Sequence Viewer

Length: 1110 bp
ATGGTTTGGAAGAAAATGGCTGCAGGCACTGATTATCCACATCCGCTCTCCATGTTGGATGGCAGTTTGAGAAAGGAGAACGTTATTGTTACAAGAGACGAGAAATCAAGTACTTTTGAGAACTTAAAGCAACCGAGTGTTGGCAAACCTCCTCGGCACCTCTCAGCTATTAGGTATTGCGTGAGCACTGCCCAGTTGGCTCCTGCAGCTGAAGTGGAGTTGGATATTGAGATTGTTAATTCAAAGTCCCCATCAAATAGTAACTCAGCTTTTGTACCTGTTTTTCGCTCTGGAAGCTGCTCTGAGATTGGATCAAAACCATTTATGGAGGATGAGCATATCTGTATAGACAATATACTTGATCATCTAGGTTCAACTGAGAACTTCACTTCTCCAGGAGCATTCTACGGGGTTTTTGATGGCCATGGTGGTACTGATGCAGCTTCTTTTATTAGAGAGAACATTTTAAGGTTTATAGTTGAGGACCCGGATTTTCCCTTCTGTACTAAGAAGGCTATTAAGAGTGCTTTCCTAAACGCTGACCTTGCTTTTGCTGATACCGGTAGTCTTGATAGTTCTTCTGGTACCACTGTACTTACTGCTCTCATTTCTGGAAGGACGATGTTAATTGCGAATGCTGGGGATTGCCGAGCTGTGCTGGGAAGAAGAGGTAGGGCAGTGGAGCTTTCCAAAGACCATAAACCCAATTGCGCCTCAGAAAGAATAAGAATTGAGAAACTGGGAGGTGTGGTGTACGATGGCTACCTCAACGGTCAATTATCGGTTGCACGAGCTCTTGGGGACTGGCACATGAAAGGCTCTAAAGGTTCCTTGTGTCCCTTGAGCGCAGAGCCGGAGCTTGAGGAGACGATTCTAAGCGAGGAAGACGAATTCATGATCCTAGGCTGTGACGGGCTGTGGGATGTGATGAGCAGCCAGTGTGCAGTCACAATCGCAAGGAAAGAACTAATGATTCACAATGATCCCGAGAGATGCTCAAGAGAGCTGGTTCGGGAGGCACTCAAGCGCAACACGTGCGATAACCTGACGGTGGTTGTGGTATGCTTCTCGCCAGATCGACCCCCGTTGCTTGATCGATCCCAAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

370

Amino Acids

40.28

Weight (kDa)

5.31

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 98 - 348 9.8e-72 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 584
AccB1I GGYRCC 2 cut(s) 156, 584
AccBSI CCGCTC 1 cut(s) 46
AciI CCGC 1 cut(s) 44
AclI AACGTT 1 cut(s) 81
AclWI GGATC 4 cut(s) 319, 892, 977, 1092
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 1 cut(s) 890
AcuI CTGAAG 1 cut(s) 231
AcvI CACGTG 1 cut(s) 1035
AfaI GTAC 7 cut(s) 112, 276, 433, 505, 586, 594, 755
AfiI CCNNNNNNNGG 2 cut(s) 140, 1051
AflIII ACRYGT 1 cut(s) 1032
AgeI ACCGGT 1 cut(s) 560
AgsI TTSAA 2 cut(s) 243, 375
AjnI CCWGG 1 cut(s) 394
Alw21I GWGCWC 2 cut(s) 188, 796
Alw26I GTCTC 2 cut(s) 90, 860
AlwI GGATC 4 cut(s) 319, 892, 977, 1092
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 986
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 5 cut(s) 20, 206, 297, 440, 933
ApoI RAATTY 1 cut(s) 890
ArsI GACNNNNNNTTYG 2 cut(s) 533, 565
AsiGI ACCGGT 1 cut(s) 560
Asp718I GGTACC 1 cut(s) 584
AspA2I CCTAGG 1 cut(s) 901
AspLEI GCGC 3 cut(s) 713, 848, 1029
AspS9I GGNCC 1 cut(s) 484
AsuC2I CCSGG 1 cut(s) 488
AvaI CYCGRG 1 cut(s) 986
AvaII GGWCC 1 cut(s) 484
AvrII CCTAGG 1 cut(s) 901
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BalI TGGCCA 1 cut(s) 423
BanI GGYRCC 2 cut(s) 156, 584
BanII GRGCYC 1 cut(s) 796
BauI CACGAG 1 cut(s) 789
BbrPI CACGTG 1 cut(s) 1035
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 2 cut(s) 188, 796
BbvI GCAGC 5 cut(s) 7, 218, 284, 452, 945
BccI CCATC 4 cut(s) 53, 259, 413, 752
BciT130I CCWGG 1 cut(s) 396
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 2 cut(s) 90, 860
BfaI CTAG 2 cut(s) 368, 902
BfmI CTRYAG 2 cut(s) 21, 204
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 5 cut(s) 21, 207, 298, 441, 934
BlnI CCTAGG 1 cut(s) 901
BlsI GCNGC 5 cut(s) 22, 208, 299, 442, 935
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 2 cut(s) 396, 488
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 986
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 5 cut(s) 158, 201, 486, 586, 829
BmrFI CCNGG 2 cut(s) 396, 488
BmrI ACTGGG 2 cut(s) 187, 749
BmsI GCATC 2 cut(s) 427, 983
BmuI ACTGGG 2 cut(s) 187, 749
BpiI GAAGAC 1 cut(s) 891
BplI GAGNNNNNCTC 2 cut(s) 980, 1012
BpmI CTGGAG 1 cut(s) 378
BpuEI CTTGAG 4 cut(s) 862, 881, 982, 1007
BpuMI CCSGG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 1096
BsaAI YACGTR 1 cut(s) 1035
BsaJI CCNNGG 3 cut(s) 152, 424, 901
BsaWI WCCGGW 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 735, 765
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 1051
Bse118I RCCGGY 1 cut(s) 560
Bse1I ACTGG 4 cut(s) 193, 744, 809, 937
BseBI CCWGG 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 1096
BseDI CCNNGG 3 cut(s) 152, 424, 901
BseGI GGATG 4 cut(s) 40, 64, 337, 928
BseLI CCNNNNNNNGG 2 cut(s) 140, 1051
BseMII CTCAG 5 cut(s) 177, 279, 294, 369, 729
BseNI ACTGG 4 cut(s) 193, 744, 809, 937
BseRI GAGGAG 2 cut(s) 141, 878
BseXI GCAGC 5 cut(s) 7, 218, 284, 452, 945
BseYI CCCAGC 2 cut(s) 638, 658
BsgI GTGCAG 1 cut(s) 963
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 2 cut(s) 156, 584
BshTI ACCGGT 1 cut(s) 560
BshVI ATCGAT 1 cut(s) 1096
BsiHKAI GWGCWC 2 cut(s) 188, 796
BsiHKCI CYCGRG 1 cut(s) 986
BsiSI CCGG 3 cut(s) 488, 561, 854
BslFI GGGAC 3 cut(s) 232, 815, 822
BslI CCNNNNNNNGG 2 cut(s) 140, 1051
BsmAI GTCTC 2 cut(s) 90, 860
BsmBI CGTCTC 2 cut(s) 90, 860
BsmFI GGGAC 3 cut(s) 232, 815, 822
BsmI GAATGC 2 cut(s) 401, 640
BsnI GGCC 1 cut(s) 423
BsoBI CYCGRG 1 cut(s) 986
Bsp1286I GDGCHC 2 cut(s) 188, 796
Bsp143I GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 1 cut(s) 44
BspANI GGCC 1 cut(s) 423
BspCNI CTCAG 5 cut(s) 176, 278, 295, 370, 728
BspDI ATCGAT 1 cut(s) 1096
BspHI TCATGA 1 cut(s) 894
BspLI GGNNCC 5 cut(s) 158, 201, 486, 586, 829
BspMAI CTGCAG 2 cut(s) 25, 208
BspPI GGATC 4 cut(s) 319, 892, 977, 1092
BspT107I GGYRCC 2 cut(s) 156, 584
BsrBI CCGCTC 1 cut(s) 46
BsrFI RCCGGY 1 cut(s) 560
BsrI ACTGG 4 cut(s) 193, 744, 809, 937
BssAI RCCGGY 1 cut(s) 560
BssECI CCNNGG 3 cut(s) 152, 424, 901
BssMI GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
BssSI CACGAG 1 cut(s) 789
BssT1I CCWWGG 2 cut(s) 424, 901
Bst2BI CACGAG 1 cut(s) 789
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 3 cut(s) 592, 773, 1051
Bst6I CTCTTC 1 cut(s) 661
BstAPI GCANNNNNTGC 1 cut(s) 1035
BstBAI YACGTR 1 cut(s) 1035
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 7 cut(s) 163, 265, 303, 378, 507, 715, 875
BstDSI CCRYGG 1 cut(s) 424
BstF5I GGATG 4 cut(s) 40, 64, 337, 928
BstHHI GCGC 3 cut(s) 713, 848, 1029
BstKTI GATC 7 cut(s) 314, 364, 900, 985, 1078, 1096, 1100
BstMAI GTCTC 2 cut(s) 90, 860
BstMBI GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
BstMWI GCNNNNNNNGC 5 cut(s) 197, 206, 294, 545, 1035
BstNI CCWGG 1 cut(s) 396
BstSCI CCNGG 2 cut(s) 394, 486
BstSFI CTRYAG 2 cut(s) 21, 204
BstV1I GCAGC 5 cut(s) 7, 218, 284, 452, 945
BstV2I GAAGAC 1 cut(s) 891
Bsu15I ATCGAT 1 cut(s) 1096
BsuRI GGCC 1 cut(s) 423
BsuTUI ATCGAT 1 cut(s) 1096
BtgI CCRYGG 1 cut(s) 424
BtsCI GGATG 4 cut(s) 40, 64, 337, 928
BtsI GCAGTG 2 cut(s) 186, 684
BtsIMutI CAGTG 5 cut(s) 27, 186, 588, 684, 944
Cac8I GCNNGC 1 cut(s) 25
CaiI CAGNNNCTG 1 cut(s) 29
CciI TCATGA 1 cut(s) 894
CfoI GCGC 3 cut(s) 713, 848, 1029
Cfr10I RCCGGY 1 cut(s) 560
Cfr13I GGNCC 1 cut(s) 484
ClaI ATCGAT 1 cut(s) 1096
Csp6I GTAC 7 cut(s) 111, 275, 432, 504, 585, 593, 754
CspAI ACCGGT 1 cut(s) 560
CviAII CATG 4 cut(s) 52, 425, 811, 895
CviQI GTAC 7 cut(s) 111, 275, 432, 504, 585, 593, 754
DdeI CTNAG 7 cut(s) 163, 265, 303, 378, 507, 715, 875
DpnI GATC 7 cut(s) 313, 363, 899, 984, 1077, 1095, 1099
DpnII GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 661
EarI CTCTTC 1 cut(s) 661
Ecl136II GAGCTC 1 cut(s) 794
Eco130I CCWWGG 2 cut(s) 424, 901
Eco24I GRGCYC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 484
Eco53kI GAGCTC 1 cut(s) 794
Eco57I CTGAAG 1 cut(s) 231
Eco72I CACGTG 1 cut(s) 1035
Eco88I CYCGRG 1 cut(s) 986
EcoICRI GAGCTC 1 cut(s) 794
EcoO109I RGGNCCY 1 cut(s) 484
EcoRI GAATTC 1 cut(s) 890
EcoRII CCWGG 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 424, 901
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 2 cut(s) 424, 901
Esp3I CGTCTC 2 cut(s) 90, 860
FaeI CATG 4 cut(s) 55, 428, 814, 898
FaqI GGGAC 3 cut(s) 232, 815, 822
FatI CATG 4 cut(s) 51, 424, 810, 894
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 5 cut(s) 21, 207, 298, 441, 934
FokI GGATG 4 cut(s) 27, 71, 344, 935
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 5 cut(s) 21, 207, 298, 441, 934
FspBI CTAG 2 cut(s) 368, 902
GlaI GCGC 3 cut(s) 712, 847, 1028
GluI GCNGC 5 cut(s) 21, 207, 298, 441, 934
GsaI CCCAGC 2 cut(s) 642, 662
GsuI CTGGAG 1 cut(s) 378
HaeIII GGCC 1 cut(s) 423
HapII CCGG 3 cut(s) 488, 561, 854
HhaI GCGC 3 cut(s) 713, 848, 1029
Hin1II CATG 4 cut(s) 55, 428, 814, 898
Hin6I GCGC 3 cut(s) 711, 846, 1027
HinP1I GCGC 3 cut(s) 711, 846, 1027
HinfI GANTC 2 cut(s) 871, 973
HpaII CCGG 3 cut(s) 488, 561, 854
Hpy166II GTNNAC 1 cut(s) 754
Hpy188I TCNGA 2 cut(s) 304, 718
Hpy188III TCNNGA 7 cut(s) 291, 569, 612, 895, 986, 999, 1013
Hpy8I GTNNAC 1 cut(s) 754
HpyAV CCTTC 3 cut(s) 505, 508, 609
HpyCH4III ACNGT 3 cut(s) 592, 773, 1051
HpyCH4IV ACGT 2 cut(s) 81, 1034
HpyCH4V TGCA 5 cut(s) 23, 206, 440, 788, 944
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 206, 294, 545, 1035
HpyF3I CTNAG 7 cut(s) 163, 265, 303, 378, 507, 715, 875
HpySE526I ACGT 2 cut(s) 81, 1034
Hsp92II CATG 4 cut(s) 55, 428, 814, 898
HspAI GCGC 3 cut(s) 711, 846, 1027
KpnI GGTACC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
LmnI GCTCC 4 cut(s) 205, 398, 682, 856
Lsp1109I GCAGC 5 cut(s) 7, 218, 284, 452, 945
LweI GCATC 2 cut(s) 427, 983
MaeI CTAG 2 cut(s) 368, 902
MaeII ACGT 2 cut(s) 81, 1034
MaeIII GTNAC 4 cut(s) 88, 260, 908, 946
MalI GATC 7 cut(s) 313, 363, 899, 984, 1077, 1095, 1099
MbiI CCGCTC 1 cut(s) 46
MboI GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
MboII GAAGA 5 cut(s) 22, 570, 675, 678, 896
MfeI CAATTG 1 cut(s) 706
MhlI GDGCHC 2 cut(s) 188, 796
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 6 cut(s) 238, 627, 706, 729, 776, 890
MluNI TGGCCA 1 cut(s) 423
MmeI TCCRAC 2 cut(s) 36, 201
Mox20I TGGCCA 1 cut(s) 423
MscI TGGCCA 1 cut(s) 423
MseI TTAA 6 cut(s) 125, 237, 467, 519, 626, 1108
Msp20I TGGCCA 1 cut(s) 423
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 3 cut(s) 488, 561, 854
MspR9I CCNGG 2 cut(s) 396, 488
MunI CAATTG 1 cut(s) 706
Mva1269I GAATGC 2 cut(s) 401, 640
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 5 cut(s) 197, 206, 294, 545, 1035
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 1 cut(s) 424
NdeII GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
NlaIII CATG 4 cut(s) 55, 428, 814, 898
NlaIV GGNNCC 5 cut(s) 158, 201, 486, 586, 829
NmeAIII GCCGAG 2 cut(s) 133, 674
NmuCI GTSAC 2 cut(s) 908, 946
PagI TCATGA 1 cut(s) 894
PctI GAATGC 2 cut(s) 401, 640
PfeI GAWTC 2 cut(s) 871, 973
PfoI TCCNGGA 1 cut(s) 394
PinAI ACCGGT 1 cut(s) 560
PkrI GCNGC 5 cut(s) 22, 208, 299, 442, 935
PmaCI CACGTG 1 cut(s) 1035
PmlI CACGTG 1 cut(s) 1035
Ppu21I YACGTR 1 cut(s) 1035
PpuMI RGGWCCY 1 cut(s) 484
Psp124BI GAGCTC 1 cut(s) 796
Psp1406I AACGTT 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 394
PspCI CACGTG 1 cut(s) 1035
PspFI CCCAGC 2 cut(s) 638, 658
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 5 cut(s) 158, 201, 486, 586, 829
PspPI GGNCC 1 cut(s) 484
PspPPI RGGWCCY 1 cut(s) 484
PstI CTGCAG 2 cut(s) 25, 208
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 7 cut(s) 112, 276, 433, 505, 586, 594, 755
RsaNI GTAC 7 cut(s) 111, 275, 432, 504, 585, 593, 754
SacI GAGCTC 1 cut(s) 796
SaqAI TTAA 6 cut(s) 125, 237, 467, 519, 626, 1108
SatI GCNGC 5 cut(s) 21, 207, 298, 441, 934
Sau3AI GATC 7 cut(s) 311, 361, 897, 982, 1075, 1093, 1097
Sau96I GGNCC 1 cut(s) 484
ScaI AGTACT 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 396, 488
SduI GDGCHC 2 cut(s) 188, 796
SfaNI GCATC 2 cut(s) 427, 983
SfcI CTRYAG 2 cut(s) 21, 204
SinI GGWCC 1 cut(s) 484
SmlI CTYRAG 4 cut(s) 841, 860, 997, 1022
SmoI CTYRAG 4 cut(s) 841, 860, 997, 1022
Sse9I AATT 6 cut(s) 238, 627, 706, 729, 776, 890
SsiI CCGC 1 cut(s) 44
SspMI CTAG 2 cut(s) 368, 902
SstI GAGCTC 1 cut(s) 796
StyD4I CCNGG 2 cut(s) 394, 486
StyI CCWWGG 2 cut(s) 424, 901
TaaI ACNGT 3 cut(s) 592, 773, 1051
TaiI ACGT 2 cut(s) 84, 1037
TaqI TCGA 2 cut(s) 1078, 1096
TasI AATT 6 cut(s) 238, 627, 706, 729, 776, 890
TatI WGTACW 3 cut(s) 110, 503, 592
TfiI GAWTC 2 cut(s) 871, 973
Tru1I TTAA 6 cut(s) 125, 237, 467, 519, 626, 1108
Tru9I TTAA 6 cut(s) 125, 237, 467, 519, 626, 1108
TscAI CASTG 5 cut(s) 34, 193, 595, 684, 944
TseFI GTSAC 2 cut(s) 908, 946
TseI GCWGC 5 cut(s) 20, 206, 297, 440, 933
Tsp45I GTSAC 2 cut(s) 908, 946
TspDTI ATGAA 2 cut(s) 827, 883
TspRI CASTG 5 cut(s) 34, 193, 595, 684, 944
VpaK11BI GGWCC 1 cut(s) 484
XapI RAATTY 1 cut(s) 890
XmaJI CCTAGG 1 cut(s) 901
XspI CTAG 2 cut(s) 368, 902
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.