MD05G1139300.v1.1

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002573
Physical Location & Seq
Reverse (-)
26978222 .. 26981332
3111 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1139300.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGCGAAAAGGAGTGTTTGAAAGGGTATACTTGAGGTCCAGTTATTTATCCGGGGGTGGATTATGTTTTGGGGAGAAAATGGCTGCAGGGACTGATTACCCGCAGGCACTTTCCATGTTGGATGGCAGTTATAGGAAGGAGAACGTTACTGTTACAAGAGAAGAGAAATCAAGTACTTTTGAGAACTTGAAGCAACCGAACATTGGCAGACCTCCTCGTCACCTCTCAGCTATTAGGTGTTGCGTGAGCTCTGCCCAGTTGGCTCCTGCAGCTGAAGTGGAGTTGGATATTGGGATTGTTAGCTCAAATTCCCCATCAGATAGTAACTCAGCTTTTGTACCTGTTTTTCGCTCTGGAAGCTGCTCGGAGATTGGACCGAAACCATATATGGAGGATGAGCATATTTGTGTAGACAATATTCTTGATCATCTAGGTGCAACTGCGAACTTCACTTCTCCTGGAGCATTCTATGGGGTTTTTGACGGCCATGGTGGTACTGATGCATCTTCGTTTATCAGAGAGAACATTTTAAGGTTTATAGTTGAGGATCCACATTTCCCGTTCTGTACTAAGAAGGCTATGAAGAGCGCTTTTCTAAATGCTGACCTTGCTTTTGCTGCTACCGGTGGTCTTGACAGTTCTTCTGGTACCACTGCACTTACGGCCCTCATCTCTGGAAGGGCGATGCTAATTGCAAATGCTGGAGATTGCCGAGCTGTGCTGGGAAGAAGAGGTAGGGCAATTGAGCTTTCCAAAGACCATAAACCCAATTGTGCCTCCGAAAGAGTGAGAATCGAGAAACTGGGAGGCATGGTGTATGATGGCTACCTCAACGGTCAATTATCTGTGGCACGAGCTCTTGGGGACTGGCACATGAAAGGCTCCAAAGGTTCCTTGTGTCCCTTGAGTGCAGAGCCAGAGCTCGAGGAGACAATTCTGAGCGAGGAAGACGAATTCTTAATCCTAGGCTGTGATGGGTTGTGGGATGTGATGAGCAGCCAGTGTGCAGTCACGATGGCAAGGAAAGAACTTATGATCCACAATGATCCCGAGAGATGCTCAAGAGAGCTGGTTCGGGAGGCACTCAAGCGCAATACGTGCGATAACCTGACGGTGGTAGTGGTATGCTTCTCGCCAGATCCACCCCCGTTGCTTGAGATCCCAAAGTTAAAATTCAAGAGGAGTATATCGGCGGAAGGCCTGAATCTTCTGCAGGAGGTACTAGATAGTAAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

413

Amino Acids

44.72

Weight (kDa)

5.62

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 128 - 369 5.6e-71 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 216
Acc65I GGTACC 1 cut(s) 647
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 2 cut(s) 27, 411
AciI CCGC 2 cut(s) 101, 1193
AclI AACGTT 1 cut(s) 144
AclWI GGATC 6 cut(s) 542, 555, 1030, 1040, 1133, 1153
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 3 cut(s) 307, 953, 1172
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 6 cut(s) 175, 339, 496, 568, 649, 1221
AfeI AGCGCT 1 cut(s) 589
AfiI CCNNNNNNNGG 2 cut(s) 203, 1114
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 3 cut(s) 20, 190, 1177
AjnI CCWGG 1 cut(s) 457
AloI GAACNNNNNNTCC 2 cut(s) 1020, 1052
Alw21I GWGCWC 3 cut(s) 251, 859, 924
Alw26I GTCTC 1 cut(s) 923
AlwI GGATC 6 cut(s) 542, 555, 1030, 1040, 1133, 1153
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 2 cut(s) 923, 1049
Aor51HI AGCGCT 1 cut(s) 589
AoxI GGCC 3 cut(s) 484, 663, 1198
ApeKI GCWGC 5 cut(s) 83, 269, 360, 617, 996
ApoI RAATTY 3 cut(s) 307, 953, 1172
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AsiGI ACCGGT 1 cut(s) 623
Asp718I GGTACC 1 cut(s) 647
AspA2I CCTAGG 1 cut(s) 964
AspLEI GCGC 2 cut(s) 590, 1092
AspS9I GGNCC 3 cut(s) 36, 374, 664
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 1 cut(s) 212
AvaI CYCGRG 2 cut(s) 923, 1049
AvaII GGWCC 2 cut(s) 36, 374
AvrII CCTAGG 1 cut(s) 964
BamHI GGATCC 1 cut(s) 547
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 3 cut(s) 251, 859, 924
BauI CACGAG 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 954
Bbv12I GWGCWC 3 cut(s) 251, 859, 924
BbvI GCAGC 5 cut(s) 70, 281, 347, 604, 1008
BccI CCATC 5 cut(s) 116, 322, 815, 968, 1009
BceAI ACGGC 2 cut(s) 499, 678
BciT130I CCWGG 1 cut(s) 459
BclI TGATCA 1 cut(s) 424
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 1 cut(s) 923
BfaI CTAG 3 cut(s) 431, 965, 1223
BfmI CTRYAG 3 cut(s) 84, 267, 1211
BfoI RGCGCY 1 cut(s) 591
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 5 cut(s) 84, 270, 361, 618, 997
BlnI CCTAGG 1 cut(s) 964
BlsI GCNGC 5 cut(s) 85, 271, 362, 619, 998
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 2 cut(s) 52, 459
Bme18I GGWCC 2 cut(s) 36, 374
BmeT110I CYCGRG 2 cut(s) 923, 1049
BmgT120I GGNCC 3 cut(s) 36, 374, 664
BmiI GGNNCC 5 cut(s) 264, 549, 649, 883, 892
BmrFI CCNGG 2 cut(s) 52, 459
BmrI ACTGGG 2 cut(s) 250, 812
BmsI GCATC 4 cut(s) 490, 512, 675, 1046
BmuI ACTGGG 2 cut(s) 250, 812
BpiI GAAGAC 1 cut(s) 954
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 2 cut(s) 480, 723
BpuEI CTTGAG 5 cut(s) 52, 925, 1045, 1070, 1175
BpuMI CCSGG 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 1098
BsaJI CCNNGG 3 cut(s) 51, 487, 964
BsaWI WCCGGW 1 cut(s) 623
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 1114
Bse118I RCCGGY 1 cut(s) 623
Bse1I ACTGG 5 cut(s) 39, 256, 807, 872, 1000
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 51, 487, 964
BseGI GGATG 3 cut(s) 127, 400, 991
BseLI CCNNNNNNNGG 2 cut(s) 203, 1114
BseMII CTCAG 3 cut(s) 240, 342, 929
BseNI ACTGG 5 cut(s) 39, 256, 807, 872, 1000
BseRI GAGGAG 3 cut(s) 204, 941, 1195
BseXI GCAGC 5 cut(s) 70, 281, 347, 604, 1008
BseYI CCCAGC 1 cut(s) 721
BsgI GTGCAG 3 cut(s) 639, 930, 1026
BshFI GGCC 3 cut(s) 486, 665, 1200
BshNI GGYRCC 1 cut(s) 647
BshTI ACCGGT 1 cut(s) 623
BsiHKAI GWGCWC 3 cut(s) 251, 859, 924
BsiHKCI CYCGRG 2 cut(s) 923, 1049
BsiSI CCGG 2 cut(s) 51, 624
BslFI GGGAC 3 cut(s) 103, 878, 885
BslI CCNNNNNNNGG 2 cut(s) 203, 1114
BsmAI GTCTC 1 cut(s) 923
BsmFI GGGAC 3 cut(s) 103, 878, 885
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 3 cut(s) 486, 665, 1200
BsoBI CYCGRG 2 cut(s) 923, 1049
Bsp1286I GDGCHC 3 cut(s) 251, 859, 924
Bsp143I GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
Bsp19I CCATGG 1 cut(s) 487
BspACI CCGC 2 cut(s) 101, 1193
BspANI GGCC 3 cut(s) 486, 665, 1200
BspCNI CTCAG 3 cut(s) 239, 341, 930
BspHI TCATGA 1 cut(s) 1235
BspLI GGNNCC 5 cut(s) 264, 549, 649, 883, 892
BspMAI CTGCAG 3 cut(s) 88, 271, 1215
BspPI GGATC 6 cut(s) 542, 555, 1030, 1040, 1133, 1153
BspQI GCTCTTC 1 cut(s) 578
BspT107I GGYRCC 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 623
BsrI ACTGG 5 cut(s) 39, 256, 807, 872, 1000
BssAI RCCGGY 1 cut(s) 623
BssECI CCNNGG 3 cut(s) 51, 487, 964
BssMI GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
BssNAI GTATAC 1 cut(s) 28
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 2 cut(s) 487, 964
Bst1107I GTATAC 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 4 cut(s) 151, 638, 836, 1114
Bst6I CTCTTC 3 cut(s) 156, 578, 724
BstAPI GCANNNNNTGC 1 cut(s) 1098
BstBAI YACGTR 1 cut(s) 1098
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 4 cut(s) 226, 328, 570, 938
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 3 cut(s) 127, 400, 991
BstH2I RGCGCY 1 cut(s) 591
BstHHI GCGC 2 cut(s) 590, 1092
BstKTI GATC 6 cut(s) 427, 550, 1038, 1048, 1141, 1161
BstMAI GTCTC 1 cut(s) 923
BstMBI GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
BstMWI GCNNNNNNNGC 7 cut(s) 260, 269, 357, 608, 617, 662, 1098
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 2 cut(s) 50, 457
BstSFI CTRYAG 3 cut(s) 84, 267, 1211
BstV1I GCAGC 5 cut(s) 70, 281, 347, 604, 1008
BstV2I GAAGAC 1 cut(s) 954
BstX2I RGATCY 3 cut(s) 547, 1138, 1158
BstYI RGATCY 3 cut(s) 547, 1138, 1158
BstZ17I GTATAC 1 cut(s) 28
BsuRI GGCC 3 cut(s) 486, 665, 1200
BtgI CCRYGG 1 cut(s) 487
BtgZI GCGATG 1 cut(s) 698
BtsCI GGATG 3 cut(s) 127, 400, 991
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 651, 1007
Cac8I GCNNGC 1 cut(s) 105
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 1235
CfoI GCGC 2 cut(s) 590, 1092
Cfr10I RCCGGY 1 cut(s) 623
Cfr13I GGNCC 3 cut(s) 36, 374, 664
Csp6I GTAC 6 cut(s) 174, 338, 495, 567, 648, 1220
CspAI ACCGGT 1 cut(s) 623
CspCI CAANNNNNGTGG 2 cut(s) 1131, 1166
CviAII CATG 5 cut(s) 115, 488, 811, 874, 1236
CviQI GTAC 6 cut(s) 174, 338, 495, 567, 648, 1220
DdeI CTNAG 4 cut(s) 226, 328, 570, 938
DpnI GATC 6 cut(s) 426, 549, 1037, 1047, 1140, 1160
DpnII GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
DrdI GACNNNNNNGTC 1 cut(s) 216
DseDI GACNNNNNNGTC 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 3 cut(s) 156, 578, 724
EarI CTCTTC 3 cut(s) 156, 578, 724
EciI GGCGGA 1 cut(s) 1208
Ecl136II GAGCTC 3 cut(s) 249, 857, 922
Eco130I CCWWGG 2 cut(s) 487, 964
Eco147I AGGCCT 1 cut(s) 1200
Eco24I GRGCYC 3 cut(s) 251, 859, 924
Eco47I GGWCC 2 cut(s) 36, 374
Eco47III AGCGCT 1 cut(s) 589
Eco53kI GAGCTC 3 cut(s) 249, 857, 922
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 2 cut(s) 923, 1049
EcoICRI GAGCTC 3 cut(s) 249, 857, 922
EcoRI GAATTC 1 cut(s) 953
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 2 cut(s) 487, 964
EcoT22I ATGCAT 1 cut(s) 505
EcoT38I GRGCYC 3 cut(s) 251, 859, 924
ErhI CCWWGG 2 cut(s) 487, 964
FaeI CATG 5 cut(s) 118, 491, 814, 877, 1239
FaqI GGGAC 3 cut(s) 103, 878, 885
FatI CATG 5 cut(s) 114, 487, 810, 873, 1235
FauI CCCGC 1 cut(s) 108
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 2 cut(s) 27, 411
Fnu4HI GCNGC 5 cut(s) 84, 270, 361, 618, 997
FokI GGATG 3 cut(s) 134, 407, 998
FriOI GRGCYC 3 cut(s) 251, 859, 924
Fsp4HI GCNGC 5 cut(s) 84, 270, 361, 618, 997
FspBI CTAG 3 cut(s) 431, 965, 1223
GlaI GCGC 2 cut(s) 589, 1091
GluI GCNGC 5 cut(s) 84, 270, 361, 618, 997
GsaI CCCAGC 1 cut(s) 725
GsuI CTGGAG 2 cut(s) 480, 723
HaeII RGCGCY 1 cut(s) 591
HaeIII GGCC 3 cut(s) 486, 665, 1200
HapII CCGG 2 cut(s) 51, 624
HhaI GCGC 2 cut(s) 590, 1092
Hin1II CATG 5 cut(s) 118, 491, 814, 877, 1239
Hin6I GCGC 2 cut(s) 588, 1090
HinP1I GCGC 2 cut(s) 588, 1090
HinfI GANTC 2 cut(s) 792, 1204
HpaII CCGG 2 cut(s) 51, 624
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 2 cut(s) 28, 412
Hpy188I TCNGA 5 cut(s) 319, 367, 518, 781, 939
Hpy8I GTNNAC 2 cut(s) 28, 412
HpyAV CCTTC 4 cut(s) 130, 568, 672, 1190
HpyCH4III ACNGT 4 cut(s) 151, 638, 836, 1114
HpyCH4IV ACGT 2 cut(s) 144, 1097
HpyCH4V TGCA 9 cut(s) 86, 269, 437, 503, 656, 695, 911, 1007, 1213
HpyF10VI GCNNNNNNNGC 7 cut(s) 260, 269, 357, 608, 617, 662, 1098
HpyF3I CTNAG 4 cut(s) 226, 328, 570, 938
HpySE526I ACGT 2 cut(s) 144, 1097
Hsp92II CATG 5 cut(s) 118, 491, 814, 877, 1239
HspAI GCGC 2 cut(s) 588, 1090
KpnI GGTACC 1 cut(s) 651
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
LguI GCTCTTC 1 cut(s) 578
LmnI GCTCC 3 cut(s) 268, 461, 887
Lsp1109I GCAGC 5 cut(s) 70, 281, 347, 604, 1008
LweI GCATC 4 cut(s) 490, 512, 675, 1046
MaeI CTAG 3 cut(s) 431, 965, 1223
MaeII ACGT 2 cut(s) 144, 1097
MaeIII GTNAC 5 cut(s) 145, 151, 218, 323, 1009
MalI GATC 6 cut(s) 426, 549, 1037, 1047, 1140, 1160
MboI GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
MboII GAAGA 8 cut(s) 173, 498, 595, 633, 738, 741, 959, 1199
MfeI CAATTG 2 cut(s) 741, 769
MflI RGATCY 3 cut(s) 547, 1138, 1158
MhlI GDGCHC 3 cut(s) 251, 859, 924
MluCI AATT 8 cut(s) 307, 690, 741, 769, 839, 933, 953, 1172
MmeI TCCRAC 2 cut(s) 99, 264
Mph1103I ATGCAT 1 cut(s) 505
MseI TTAA 3 cut(s) 530, 959, 1169
MslI CAYNNNNRTG 2 cut(s) 405, 432
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 2 cut(s) 51, 624
MspR9I CCNGG 2 cut(s) 52, 459
MunI CAATTG 2 cut(s) 741, 769
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 7 cut(s) 260, 269, 357, 608, 617, 662, 1098
NciI CCSGG 1 cut(s) 52
NcoI CCATGG 1 cut(s) 487
NdeII GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
NlaIII CATG 5 cut(s) 118, 491, 814, 877, 1239
NlaIV GGNNCC 5 cut(s) 264, 549, 649, 883, 892
NmeAIII GCCGAG 1 cut(s) 737
NmuCI GTSAC 2 cut(s) 218, 1009
NsiI ATGCAT 1 cut(s) 505
PaeR7I CTCGAG 1 cut(s) 923
PagI TCATGA 1 cut(s) 1235
PceI AGGCCT 1 cut(s) 1200
PciSI GCTCTTC 1 cut(s) 578
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 792, 1204
PfoI TCCNGGA 1 cut(s) 457
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 5 cut(s) 85, 271, 362, 619, 998
Ppu21I YACGTR 1 cut(s) 1098
Psp124BI GAGCTC 3 cut(s) 251, 859, 924
Psp1406I AACGTT 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 721
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 5 cut(s) 264, 549, 649, 883, 892
PspPI GGNCC 3 cut(s) 36, 374, 664
PspXI VCTCGAGB 1 cut(s) 923
PstI CTGCAG 3 cut(s) 88, 271, 1215
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 3 cut(s) 547, 1138, 1158
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 6 cut(s) 175, 339, 496, 568, 649, 1221
RsaNI GTAC 6 cut(s) 174, 338, 495, 567, 648, 1220
RseI CAYNNNNRTG 2 cut(s) 405, 432
SacI GAGCTC 3 cut(s) 251, 859, 924
SapI GCTCTTC 1 cut(s) 578
SaqAI TTAA 3 cut(s) 530, 959, 1169
SatI GCNGC 5 cut(s) 84, 270, 361, 618, 997
Sau3AI GATC 6 cut(s) 424, 547, 1035, 1045, 1138, 1158
Sau96I GGNCC 3 cut(s) 36, 374, 664
ScaI AGTACT 1 cut(s) 175
ScrFI CCNGG 2 cut(s) 52, 459
SduI GDGCHC 3 cut(s) 251, 859, 924
SfaNI GCATC 4 cut(s) 490, 512, 675, 1046
SfcI CTRYAG 3 cut(s) 84, 267, 1211
Sfr274I CTCGAG 1 cut(s) 923
SinI GGWCC 2 cut(s) 36, 374
SlaI CTCGAG 1 cut(s) 923
SmiMI CAYNNNNRTG 2 cut(s) 405, 432
SmlI CTYRAG 6 cut(s) 31, 904, 923, 1060, 1085, 1154
SmoI CTYRAG 6 cut(s) 31, 904, 923, 1060, 1085, 1154
Sse9I AATT 8 cut(s) 307, 690, 741, 769, 839, 933, 953, 1172
SseBI AGGCCT 1 cut(s) 1200
SsiI CCGC 2 cut(s) 101, 1193
SspI AATATT 1 cut(s) 418
SspMI CTAG 3 cut(s) 431, 965, 1223
SstI GAGCTC 3 cut(s) 251, 859, 924
StuI AGGCCT 1 cut(s) 1200
StyD4I CCNGG 2 cut(s) 50, 457
StyI CCWWGG 2 cut(s) 487, 964
TaaI ACNGT 4 cut(s) 151, 638, 836, 1114
TaiI ACGT 2 cut(s) 147, 1100
TaqI TCGA 2 cut(s) 795, 924
TaqII GACCGA 1 cut(s) 391
TasI AATT 8 cut(s) 307, 690, 741, 769, 839, 933, 953, 1172
TatI WGTACW 2 cut(s) 173, 566
TfiI GAWTC 2 cut(s) 792, 1204
Tru1I TTAA 3 cut(s) 530, 959, 1169
Tru9I TTAA 3 cut(s) 530, 959, 1169
TscAI CASTG 2 cut(s) 658, 1007
TseFI GTSAC 2 cut(s) 218, 1009
TseI GCWGC 5 cut(s) 83, 269, 360, 617, 996
Tsp45I GTSAC 2 cut(s) 218, 1009
TspDTI ATGAA 2 cut(s) 596, 890
TspRI CASTG 2 cut(s) 658, 1007
VpaK11BI GGWCC 2 cut(s) 36, 374
XapI RAATTY 3 cut(s) 307, 953, 1172
XhoI CTCGAG 1 cut(s) 923
XmaJI CCTAGG 1 cut(s) 964
XmiI GTMKAC 2 cut(s) 27, 411
XspI CTAG 3 cut(s) 431, 965, 1223
ZrmI AGTACT 1 cut(s) 175
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.