Rroxscaffold_7G00187500

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002573
Physical Location & Seq
Forward (+)
26901248 .. 26904475
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00187500.1

Sequence Viewer

Length: 960 bp
ATGGCTGCAGGGACAGATACCCCGTCTTCACTTTCCATGTCAGATGGCAGTTACAGAAAGAACGTAACTGTTACAAGGGACGAGAAATCAAATACTTTTGAGAACATGAAGCAATCCAACATCGGCAAACCTCCTAGGCATCTTTCAGCTATTAGGAACTGTGTCAGCTCTGCCCAGTTGACTCCTGCAACTGAAGCGGAGTTCGATAGTGGGATTGTTAGCTCAAAATCCTCGTCAGATAGATCTACTGCAGACTTTCCTTCTCCTGGAGCTTTCTATGGGGTTTTTGATGGCCATGGTGGTACAGATGCAGCTTCTTATATCAGAGAGAATATTTTGAGGTTTATAGTGGAGGATTCACATTTCCCTTTTTGTGCGAAGCAAGCTATTAAGAGTGCTTTTCTAAAGGCTGACCATGCTTTTGCTGATAATGGTACTCTTGATAGTTCCTCTGGTACCACTGCACTTATTGCCCTTATCTCTGGCAGGACGATGCTAGTTGCCAATGCTGGAGATTGTCGAGCTGTTCTGGGAAGAAGAGGCAGGGCAGTGGAACTTTCCAAAGACCATAAACCAAATTGTGCTTCAGAAAGAGTAAGAATTGAAGAACTGGGAGGCATGGTGTATGATGGATACCTCAACGGCCAATTGTCAGTGGCACGAGCACTTGGAGATTGGCACATGAAAGGGTCCAAAGGTTCCTCTTGTCCCTTAAGTGCAGAACCAGAGCTTGAGGAGACAATTCTGAGTGAGGAAGATGAGTTCTTGATCATAGGCTGTGATGGGCTGTGGGATGTGATGAGCAGCCAGTGTGCCGTCACAATTGCAAGGAAAGAGTTGATGCTTCACAATGATCCTGAAAGATGCTCCAGAGAGCTAGTTAGGGAAGCTCTCAAGCGCGATACACGTGATAACTTGACCGTTGTAGTGGTATGCTTCTCCCCCAGAACCACCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

319

Amino Acids

34.5

Weight (kDa)

5.59

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 85 - 305 2.3e-72 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 455
AccB1I GGYRCC 1 cut(s) 455
AccII CGCG 1 cut(s) 900
AciI CCGC 2 cut(s) 197, 954
AclWI GGATC 1 cut(s) 848
AcoI YGGCCR 2 cut(s) 292, 643
AcuI CTGAAG 2 cut(s) 213, 570
AcvI CACGTG 1 cut(s) 908
AfaI GTAC 3 cut(s) 304, 436, 457
AfiI CCNNNNNNNGG 1 cut(s) 266
AflII CTTAAG 1 cut(s) 712
AflIII ACRYGT 1 cut(s) 905
AgsI TTSAA 1 cut(s) 605
AjnI CCWGG 1 cut(s) 265
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AloI GAACNNNNNNTCC 2 cut(s) 746, 778
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 731
AlwI GGATC 1 cut(s) 848
AoxI GGCC 2 cut(s) 292, 643
ApeKI GCWGC 3 cut(s) 5, 311, 804
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 404, 436
Asp718I GGTACC 1 cut(s) 455
AspA2I CCTAGG 1 cut(s) 134
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 1 cut(s) 690
AvaII GGWCC 1 cut(s) 690
AvrII CCTAGG 1 cut(s) 134
BalI TGGCCA 1 cut(s) 294
BanI GGYRCC 1 cut(s) 455
BauI CACGAG 1 cut(s) 660
BbrPI CACGTG 1 cut(s) 908
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 323, 816
BccI CCATC 4 cut(s) 38, 284, 623, 776
BceAI ACGGC 2 cut(s) 658, 800
BciT130I CCWGG 1 cut(s) 267
BciVI GTATCC 1 cut(s) 626
BclI TGATCA 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 731
BfaI CTAG 3 cut(s) 135, 497, 878
BfmI CTRYAG 2 cut(s) 6, 249
BfrI CTTAAG 1 cut(s) 712
BfuI GTATCC 1 cut(s) 626
BglII AGATCT 1 cut(s) 242
BisI GCNGC 3 cut(s) 6, 312, 805
BlnI CCTAGG 1 cut(s) 134
BlsI GCNGC 3 cut(s) 7, 313, 806
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 690
BmiI GGNNCC 3 cut(s) 457, 691, 700
BmrFI CCNGG 1 cut(s) 267
BmrI ACTGGG 2 cut(s) 169, 620
BmsI GCATC 5 cut(s) 148, 298, 483, 831, 854
BmuI ACTGGG 2 cut(s) 169, 620
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 3 cut(s) 288, 531, 853
BpuEI CTTGAG 2 cut(s) 752, 878
BsaAI YACGTR 1 cut(s) 908
BsaJI CCNNGG 2 cut(s) 134, 295
BsaXI ACNNNNNCTCC 2 cut(s) 606, 636
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 175, 615, 808
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 134, 295
BseGI GGATG 1 cut(s) 799
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 737
BseNI ACTGG 3 cut(s) 175, 615, 808
BseRI GAGGAG 1 cut(s) 749
BseXI GCAGC 2 cut(s) 323, 816
BsgI GTGCAG 2 cut(s) 447, 738
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 2 cut(s) 294, 645
BshNI GGYRCC 1 cut(s) 455
BsiHKAI GWGCWC 1 cut(s) 667
BslFI GGGAC 3 cut(s) 25, 92, 693
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 1 cut(s) 731
BsmFI GGGAC 3 cut(s) 25, 92, 693
BsnI GGCC 2 cut(s) 294, 645
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 3 cut(s) 242, 768, 853
Bsp19I CCATGG 1 cut(s) 295
BspACI CCGC 2 cut(s) 197, 954
BspANI GGCC 2 cut(s) 294, 645
BspCNI CTCAG 1 cut(s) 738
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 3 cut(s) 457, 691, 700
BspMAI CTGCAG 2 cut(s) 10, 253
BspPI GGATC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 455
BspTI CTTAAG 1 cut(s) 712
BsrI ACTGG 3 cut(s) 175, 615, 808
BssECI CCNNGG 2 cut(s) 134, 295
BssMI GATC 3 cut(s) 242, 768, 853
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 134, 295
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 70, 161, 922
Bst6I CTCTTC 1 cut(s) 532
BstAFI CTTAAG 1 cut(s) 712
BstAPI GCANNNNNTGC 1 cut(s) 470
BstBAI YACGTR 1 cut(s) 908
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 1 cut(s) 746
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 1 cut(s) 799
BstFNI CGCG 1 cut(s) 900
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 3 cut(s) 245, 771, 856
BstMAI GTCTC 1 cut(s) 731
BstMBI GATC 3 cut(s) 242, 768, 853
BstMWI GCNNNNNNNGC 4 cut(s) 194, 383, 416, 470
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 1 cut(s) 265
BstSFI CTRYAG 2 cut(s) 6, 249
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 2 cut(s) 323, 816
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BsuI GTATCC 1 cut(s) 626
BsuRI GGCC 2 cut(s) 294, 645
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 799
BtsI GCAGTG 2 cut(s) 459, 555
BtsIMutI CAGTG 4 cut(s) 459, 555, 660, 815
Cac8I GCNNGC 1 cut(s) 384
CfoI GCGC 1 cut(s) 900
Cfr13I GGNCC 1 cut(s) 690
Csp6I GTAC 3 cut(s) 303, 435, 456
CviAII CATG 6 cut(s) 37, 106, 296, 416, 619, 682
CviQI GTAC 3 cut(s) 303, 435, 456
DdeI CTNAG 1 cut(s) 746
DpnI GATC 3 cut(s) 244, 770, 855
DpnII GATC 3 cut(s) 242, 768, 853
EaeI YGGCCR 2 cut(s) 292, 643
Eam1104I CTCTTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 532
Eco130I CCWWGG 2 cut(s) 134, 295
Eco47I GGWCC 1 cut(s) 690
Eco57I CTGAAG 2 cut(s) 213, 570
Eco72I CACGTG 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 134, 295
ErhI CCWWGG 2 cut(s) 134, 295
FaeI CATG 6 cut(s) 40, 109, 299, 419, 622, 685
FaqI GGGAC 3 cut(s) 25, 92, 693
FatI CATG 6 cut(s) 36, 105, 295, 415, 618, 681
FbaI TGATCA 1 cut(s) 768
Fnu4HI GCNGC 3 cut(s) 6, 312, 805
FokI GGATG 1 cut(s) 806
Fsp4HI GCNGC 3 cut(s) 6, 312, 805
FspBI CTAG 3 cut(s) 135, 497, 878
GlaI GCGC 1 cut(s) 899
GluI GCNGC 3 cut(s) 6, 312, 805
GsuI CTGGAG 3 cut(s) 288, 531, 853
HaeIII GGCC 2 cut(s) 294, 645
HhaI GCGC 1 cut(s) 900
Hin1II CATG 6 cut(s) 40, 109, 299, 419, 622, 685
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 1 cut(s) 180
HindII GTYRAC 1 cut(s) 180
HinfI GANTC 2 cut(s) 181, 356
Hpy166II GTNNAC 1 cut(s) 180
Hpy188I TCNGA 5 cut(s) 43, 238, 326, 589, 747
Hpy188III TCNNGA 4 cut(s) 440, 766, 857, 870
Hpy8I GTNNAC 1 cut(s) 180
HpyAV CCTTC 1 cut(s) 270
HpyCH4III ACNGT 3 cut(s) 70, 161, 922
HpyCH4IV ACGT 2 cut(s) 63, 907
HpyCH4V TGCA 7 cut(s) 8, 188, 251, 311, 464, 719, 827
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 383, 416, 470
HpyF3I CTNAG 1 cut(s) 746
HpySE526I ACGT 2 cut(s) 63, 907
Hsp92II CATG 6 cut(s) 40, 109, 299, 419, 622, 685
HspAI GCGC 1 cut(s) 898
KpnI GGTACC 1 cut(s) 459
Ksp22I TGATCA 1 cut(s) 768
Kzo9I GATC 3 cut(s) 242, 768, 853
LmnI GCTCC 2 cut(s) 269, 872
Lsp1109I GCAGC 2 cut(s) 323, 816
LweI GCATC 5 cut(s) 148, 298, 483, 831, 854
MaeI CTAG 3 cut(s) 135, 497, 878
MaeII ACGT 2 cut(s) 63, 907
MaeIII GTNAC 4 cut(s) 50, 64, 70, 817
MalI GATC 3 cut(s) 244, 770, 855
MboI GATC 3 cut(s) 242, 768, 853
MboII GAAGA 5 cut(s) 18, 546, 549, 617, 767
MfeI CAATTG 2 cut(s) 647, 822
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 667
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 5 cut(s) 577, 600, 647, 741, 822
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 1 cut(s) 175
MmeI TCCRAC 1 cut(s) 141
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
MseI TTAA 2 cut(s) 390, 713
Msp20I TGGCCA 1 cut(s) 294
MspCI CTTAAG 1 cut(s) 712
MspR9I CCNGG 1 cut(s) 267
MunI CAATTG 2 cut(s) 647, 822
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 4 cut(s) 194, 383, 416, 470
NcoI CCATGG 1 cut(s) 295
NdeII GATC 3 cut(s) 242, 768, 853
NlaIII CATG 6 cut(s) 40, 109, 299, 419, 622, 685
NlaIV GGNNCC 3 cut(s) 457, 691, 700
NmuCI GTSAC 1 cut(s) 817
PfeI GAWTC 1 cut(s) 356
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 3 cut(s) 7, 313, 806
PleI GAGTC 1 cut(s) 175
PmaCI CACGTG 1 cut(s) 908
PmlI CACGTG 1 cut(s) 908
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 908
Psp6I CCWGG 1 cut(s) 265
PspCI CACGTG 1 cut(s) 908
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 3 cut(s) 457, 691, 700
PspPI GGNCC 1 cut(s) 690
PstI CTGCAG 2 cut(s) 10, 253
PsuI RGATCY 1 cut(s) 242
RsaI GTAC 3 cut(s) 304, 436, 457
RsaNI GTAC 3 cut(s) 303, 435, 456
SaqAI TTAA 2 cut(s) 390, 713
SatI GCNGC 3 cut(s) 6, 312, 805
Sau3AI GATC 3 cut(s) 242, 768, 853
Sau96I GGNCC 1 cut(s) 690
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 5 cut(s) 148, 298, 483, 831, 854
SfcI CTRYAG 2 cut(s) 6, 249
SinI GGWCC 1 cut(s) 690
SmlI CTYRAG 3 cut(s) 712, 731, 893
SmoI CTYRAG 3 cut(s) 712, 731, 893
Sse9I AATT 5 cut(s) 577, 600, 647, 741, 822
SsiI CCGC 2 cut(s) 197, 954
SspI AATATT 1 cut(s) 334
SspMI CTAG 3 cut(s) 135, 497, 878
StyD4I CCNGG 1 cut(s) 265
StyI CCWWGG 2 cut(s) 134, 295
TaaI ACNGT 3 cut(s) 70, 161, 922
TaiI ACGT 2 cut(s) 66, 910
TaqI TCGA 2 cut(s) 204, 520
TasI AATT 5 cut(s) 577, 600, 647, 741, 822
TfiI GAWTC 1 cut(s) 356
Tru1I TTAA 2 cut(s) 390, 713
Tru9I TTAA 2 cut(s) 390, 713
TscAI CASTG 4 cut(s) 466, 555, 660, 815
TseFI GTSAC 1 cut(s) 817
TseI GCWGC 3 cut(s) 5, 311, 804
Tsp45I GTSAC 1 cut(s) 817
TspDTI ATGAA 2 cut(s) 122, 698
TspRI CASTG 4 cut(s) 466, 555, 660, 815
Vha464I CTTAAG 1 cut(s) 712
VpaK11BI GGWCC 1 cut(s) 690
XmaJI CCTAGG 1 cut(s) 134
XspI CTAG 3 cut(s) 135, 497, 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.