MD05G1157400.v1.1

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007537
Physical Location & Seq
Forward (+)
28694362 .. 28695302
941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1157400.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGATTTTGTGGTTTCATACTTTTCGAAGGTAATGGATTTTGTATCTGCCGACATTCACCTCTCCCAGACCCTGCGTAAAGCGGGAGCCTTGTGCACTGGCTTCCCTGCTCTACAGGAACTTGAAATTTGGGCCCAGAATGAGTACAAGGCTGTTGTGGGAGAAGTAAACTATGGGTCGGATGACAACCAGAATTGCCAATTGACCAAACTGCGAATTGTCAAAATAACCGGCATCTTTGGTGTCAAAGCTGAACTAGATTTCATCAGGTTTTTGCTTTCAAGTTCACCTGTGCTTGAGAAGATGACTGTGAAGCCTGCTGCTGCTAATAATTTTGAAGTAAAAATGTTGCTCCAAAAGTTGCTCCAGTTTAAGCGTGTCTCGGCGAATGCAGAGATATTCTACTTAGACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

139

Amino Acids

15.53

Weight (kDa)

6.57

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 66 - 103 7.7e-08 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 83
AcsI RAATTY 1 cut(s) 126
AfaI GTAC 1 cut(s) 146
AgsI TTSAA 3 cut(s) 125, 282, 338
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 385
Alw44I GTGCAC 1 cut(s) 94
AlwNI CAGNNNCTG 1 cut(s) 73
AoxI GGCC 1 cut(s) 132
ApaI GGGCCC 1 cut(s) 136
ApaLI GTGCAC 1 cut(s) 94
ApeKI GCWGC 2 cut(s) 320, 323
ApoI RAATTY 1 cut(s) 126
AspS9I GGNCC 2 cut(s) 132, 133
AsuHPI GGTGA 2 cut(s) 50, 279
AsuII TTCGAA 1 cut(s) 26
BaeGI GKGCMC 2 cut(s) 98, 136
BanII GRGCYC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 2 cut(s) 307, 310
BcoDI GTCTC 1 cut(s) 385
BfaI CTAG 1 cut(s) 257
BfmI CTRYAG 1 cut(s) 113
BisI GCNGC 2 cut(s) 321, 324
BlsI GCNGC 2 cut(s) 322, 325
BmgT120I GGNCC 2 cut(s) 132, 133
BmiI GGNNCC 2 cut(s) 88, 134
BmsI GCATC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 350
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 317
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 103, 367
BseGI GGATG 1 cut(s) 187
BseNI ACTGG 2 cut(s) 103, 367
BseSI GKGCMC 2 cut(s) 98, 136
BseXI GCAGC 2 cut(s) 307, 310
BshFI GGCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 385
BsmI GAATGC 1 cut(s) 394
BsnI GGCC 1 cut(s) 134
Bsp119I TTCGAA 1 cut(s) 26
Bsp120I GGGCCC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 98, 136
BspACI CCGC 1 cut(s) 83
BspANI GGCC 1 cut(s) 134
BspLI GGNNCC 2 cut(s) 88, 134
BspT104I TTCGAA 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 2 cut(s) 103, 367
BssAI RCCGGY 1 cut(s) 230
Bst4CI ACNGT 1 cut(s) 310
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 1 cut(s) 406
BstF5I GGATG 1 cut(s) 187
BstMAI GTCTC 1 cut(s) 385
BstSFI CTRYAG 1 cut(s) 113
BstSLI GKGCMC 2 cut(s) 98, 136
BstV1I GCAGC 2 cut(s) 307, 310
BsuRI GGCC 1 cut(s) 134
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 318
CaiI CAGNNNCTG 1 cut(s) 73
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 2 cut(s) 132, 133
Csp6I GTAC 1 cut(s) 145
CviJI RGCY 6 cut(s) 89, 102, 134, 152, 251, 316
CviKI_1 RGCY 6 cut(s) 89, 102, 134, 152, 251, 316
CviQI GTAC 1 cut(s) 145
DdeI CTNAG 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 136
FaiI YATR 2 cut(s) 19, 174
FauI CCCGC 1 cut(s) 76
Fnu4HI GCNGC 2 cut(s) 321, 324
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 2 cut(s) 321, 324
FspBI CTAG 1 cut(s) 257
GluI GCNGC 2 cut(s) 321, 324
GsuI CTGGAG 1 cut(s) 350
HaeIII GGCC 1 cut(s) 134
HapII CCGG 1 cut(s) 231
HpaII CCGG 1 cut(s) 231
HphI GGTGA 2 cut(s) 50, 279
Hpy166II GTNNAC 3 cut(s) 96, 169, 287
Hpy188I TCNGA 1 cut(s) 181
Hpy8I GTNNAC 3 cut(s) 96, 169, 287
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4V TGCA 2 cut(s) 96, 392
HpyF3I CTNAG 1 cut(s) 406
LmnI GCTCC 3 cut(s) 86, 357, 369
Lsp1109I GCAGC 2 cut(s) 307, 310
LweI GCATC 1 cut(s) 243
MaeI CTAG 1 cut(s) 257
MboII GAAGA 1 cut(s) 313
MfeI CAATTG 1 cut(s) 200
MhlI GDGCHC 2 cut(s) 98, 136
MluCI AATT 5 cut(s) 126, 193, 200, 216, 331
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 1 cut(s) 71
MseI TTAA 1 cut(s) 372
MspI CCGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 200
Mva1269I GAATGC 1 cut(s) 394
NlaIV GGNNCC 2 cut(s) 88, 134
NmeAIII GCCGAG 1 cut(s) 362
NspV TTCGAA 1 cut(s) 26
PctI GAATGC 1 cut(s) 394
PkrI GCNGC 2 cut(s) 322, 325
PspN4I GGNNCC 2 cut(s) 88, 134
PspOMI GGGCCC 1 cut(s) 132
PspPI GGNCC 2 cut(s) 132, 133
PstNI CAGNNNCTG 1 cut(s) 73
RsaI GTAC 1 cut(s) 146
RsaNI GTAC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 321, 324
Sau96I GGNCC 2 cut(s) 132, 133
SduI GDGCHC 2 cut(s) 98, 136
SetI ASST 5 cut(s) 33, 63, 253, 272, 292
SfaNI GCATC 1 cut(s) 243
SfcI CTRYAG 1 cut(s) 113
SfuI TTCGAA 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 5 cut(s) 126, 193, 200, 216, 331
SsiI CCGC 1 cut(s) 83
SspMI CTAG 1 cut(s) 257
TaaI ACNGT 1 cut(s) 310
TaqI TCGA 1 cut(s) 26
TasI AATT 5 cut(s) 126, 193, 200, 216, 331
TatI WGTACW 1 cut(s) 144
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 103
TseI GCWGC 2 cut(s) 320, 323
TspDTI ATGAA 2 cut(s) 6, 253
TspRI CASTG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 94
XapI RAATTY 1 cut(s) 126
XspI CTAG 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.