MD05G1222100.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004498
Physical Location & Seq
Forward (+)
35458520 .. 35463215
4696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1222100.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGAGAGTCAGAGTGGTAGAAAACAAGGTTCTACGTCTCCATGCGCAGCATGCAAACTTCTGCGAAGGAGATGTGCTCAGGATTGTGTTTTTGCTCCTTATTTTCCAGCTGACGAGCCCCAGAAGTTTGCCAGTGTCCACAAGGTTTTTGGTGCTAGCAATGTCAACAAGATGCTACAGGAATTACCAGAGCACCAACGCAGTGATGCAGTGAGTTCCATGGTGTATGAAGCAAATGCAAGAGTCCGGGATCCTGTGTACGGTTGTGTTGGAGCAATATCATCTTTACAACAACAAATCGACGCACTCCAAACCCAATTGGCCATTGCACAAGCTGAAGTGGTGCACATGAGAATGGGCCAATTCGCTATTACCACTACTACTGCTTCAACGACTACTTGCTCACCAGACCAAAAGATGGCACCTGATAATTCTCATCACCACCATCATCATCGCAGTCATCAGACGGCTACCACCAAGTCCTTTTTTTCAATGGACATGGCGGCCGATCAGGCCAATATGGGAGAGTCTTTGTGGTCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

182

Amino Acids

19.92

Weight (kDa)

7.13

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 14 - 111 8.8e-44 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 421
AccB7I CCANNNNNTGG 1 cut(s) 418
AciI CCGC 1 cut(s) 503
AclWI GGATC 2 cut(s) 245, 258
AcoI YGGCCR 2 cut(s) 321, 504
AcuI CTGAAG 1 cut(s) 357
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 2 cut(s) 260, 418
AgsI TTSAA 2 cut(s) 390, 492
AluBI AGCT 2 cut(s) 110, 335
AluI AGCT 2 cut(s) 110, 335
Alw21I GWGCWC 3 cut(s) 79, 195, 348
Alw26I GTCTC 1 cut(s) 42
Alw44I GTGCAC 1 cut(s) 344
AlwI GGATC 2 cut(s) 245, 258
AoxI GGCC 4 cut(s) 321, 358, 504, 513
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 396, 431
AsuNHI GCTAGC 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 348
BalI TGGCCA 1 cut(s) 323
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 120
Bbv12I GWGCWC 3 cut(s) 79, 195, 348
BbvI GCAGC 1 cut(s) 59
BccI CCATC 2 cut(s) 412, 453
BceAI ACGGC 1 cut(s) 483
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 2 cut(s) 156, 544
BfmI CTRYAG 1 cut(s) 176
BglI GCCNNNNNGGC 1 cut(s) 512
BisI GCNGC 2 cut(s) 48, 504
BlsI GCNGC 2 cut(s) 49, 505
Bme1390I CCNGG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 252, 423
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 162, 196
BmtI GCTAGC 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 61, 93
Bpu10I CCTNAGC 1 cut(s) 78
BpuMI CCSGG 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 418
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 2 cut(s) 166, 324
BseDI CCNNGG 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 260, 418
BseMI GCAATG 2 cut(s) 166, 324
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 132
BseSI GKGCMC 1 cut(s) 348
BseX3I CGGCCG 1 cut(s) 504
BseXI GCAGC 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 4 cut(s) 323, 360, 506, 515
BshNI GGYRCC 1 cut(s) 421
BsiEI CGRYCG 1 cut(s) 507
BsiHKAI GWGCWC 3 cut(s) 79, 195, 348
BsiSI CCGG 1 cut(s) 247
BslI CCNNNNNNNGG 2 cut(s) 260, 418
BsmAI GTCTC 1 cut(s) 42
BsmBI CGTCTC 1 cut(s) 42
BsnI GGCC 4 cut(s) 323, 360, 506, 515
Bsp1286I GDGCHC 4 cut(s) 79, 120, 195, 348
Bsp143I GATC 2 cut(s) 250, 508
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 1 cut(s) 503
BspANI GGCC 4 cut(s) 323, 360, 506, 515
BspCNI CTCAG 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 252, 423
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 2 cut(s) 245, 258
BspT107I GGYRCC 1 cut(s) 421
BsrDI GCAATG 2 cut(s) 166, 324
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 250, 508
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 52, 157
BstDEI CTNAG 1 cut(s) 78
BstDSI CCRYGG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 2 cut(s) 253, 511
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 2 cut(s) 250, 508
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 2 cut(s) 51, 512
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 176
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BstZI CGGCCG 1 cut(s) 504
BsuRI GGCC 4 cut(s) 323, 360, 506, 515
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 437
BtsI GCAGTG 2 cut(s) 208, 216
BtsIMutI CAGTG 3 cut(s) 139, 208, 216
Cac8I GCNNGC 2 cut(s) 52, 157
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 358
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 259
CviAII CATG 6 cut(s) 42, 51, 220, 349, 499, 540
CviJI RGCY 8 cut(s) 110, 118, 323, 335, 360, 470, 506, 515
CviKI_1 RGCY 8 cut(s) 110, 118, 323, 335, 360, 470, 506, 515
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 78
DpnI GATC 2 cut(s) 252, 510
DpnII GATC 2 cut(s) 250, 508
EaeI YGGCCR 2 cut(s) 321, 504
EagI CGGCCG 1 cut(s) 504
EclXI CGGCCG 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 120
Eco52I CGGCCG 1 cut(s) 504
Eco57I CTGAAG 1 cut(s) 357
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 6 cut(s) 45, 54, 223, 352, 502, 543
FaiI YATR 8 cut(s) 43, 52, 221, 228, 350, 500, 521, 541
FatI CATG 6 cut(s) 41, 50, 219, 348, 498, 539
Fnu4HI GCNGC 2 cut(s) 48, 504
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 2 cut(s) 48, 504
FspBI CTAG 2 cut(s) 156, 544
FspI TGCGCA 1 cut(s) 46
GlaI GCGC 1 cut(s) 46
GluI GCNGC 2 cut(s) 48, 504
HaeIII GGCC 4 cut(s) 323, 360, 506, 515
HapII CCGG 1 cut(s) 247
HgaI GACGC 1 cut(s) 311
HhaI GCGC 1 cut(s) 47
Hin1II CATG 6 cut(s) 45, 54, 223, 352, 502, 543
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 3 cut(s) 7, 243, 527
HpaII CCGG 1 cut(s) 247
HphI GGTGA 2 cut(s) 396, 431
Hpy166II GTNNAC 4 cut(s) 139, 166, 259, 346
Hpy188I TCNGA 2 cut(s) 12, 465
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 4 cut(s) 139, 166, 259, 346
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 1 cut(s) 60
HpyCH4III ACNGT 1 cut(s) 263
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 5 cut(s) 54, 209, 239, 329, 346
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 512
HpyF3I CTNAG 1 cut(s) 78
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 6 cut(s) 45, 54, 223, 352, 502, 543
HspAI GCGC 1 cut(s) 45
Kzo9I GATC 2 cut(s) 250, 508
LmnI GCTCC 2 cut(s) 100, 272
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 2 cut(s) 162, 196
MaeI CTAG 2 cut(s) 156, 544
MaeII ACGT 1 cut(s) 35
MalI GATC 2 cut(s) 252, 510
MboI GATC 2 cut(s) 250, 508
MfeI CAATTG 1 cut(s) 317
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 4 cut(s) 79, 120, 195, 348
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 4 cut(s) 182, 317, 362, 430
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 3 cut(s) 16, 252, 536
MmeI TCCRAC 1 cut(s) 250
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MslI CAYNNNNRTG 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 51, 512
NciI CCSGG 1 cut(s) 248
NcoI CCATGG 1 cut(s) 219
NdeII GATC 2 cut(s) 250, 508
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 6 cut(s) 45, 54, 223, 352, 502, 543
NlaIV GGNNCC 2 cut(s) 252, 423
NsbI TGCGCA 1 cut(s) 46
NspI RCATGY 1 cut(s) 54
PaeI GCATGC 1 cut(s) 54
PflMI CCANNNNNTGG 1 cut(s) 418
PfoI TCCNGGA 1 cut(s) 246
PkrI GCNGC 2 cut(s) 49, 505
PleI GAGTC 3 cut(s) 15, 251, 535
PpsI GAGTC 3 cut(s) 15, 251, 535
PspN4I GGNNCC 2 cut(s) 252, 423
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 1 cut(s) 250
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 1 cut(s) 353
SatI GCNGC 2 cut(s) 48, 504
Sau3AI GATC 2 cut(s) 250, 508
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 3 cut(s) 16, 252, 536
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 4 cut(s) 79, 120, 195, 348
SetI ASST 6 cut(s) 31, 38, 112, 147, 337, 427
SfaNI GCATC 2 cut(s) 162, 196
SfcI CTRYAG 1 cut(s) 176
SfiI GGCCNNNNNGGCC 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 353
SphI GCATGC 1 cut(s) 54
Sse9I AATT 4 cut(s) 182, 317, 362, 430
SsiI CCGC 1 cut(s) 503
SspMI CTAG 2 cut(s) 156, 544
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 263
TaiI ACGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 300
TasI AATT 4 cut(s) 182, 317, 362, 430
TauI GCSGC 1 cut(s) 506
TscAI CASTG 3 cut(s) 139, 208, 216
TseI GCWGC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 243
TspRI CASTG 3 cut(s) 139, 208, 216
Van91I CCANNNNNTGG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 344
XceI RCATGY 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 1 cut(s) 146
XspI CTAG 2 cut(s) 156, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.