MD05G1259100.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004498
Physical Location & Seq
Forward (+)
39494307 .. 39496318
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1259100.v1.1.491

Sequence Viewer

Length: 528 bp
ATGAAGGAGAATGGCAGCAGCAGGAAACAAGGTGCACCCTCGCCCTGTGCAGCATGCAAGCTTCTCAGAAGAAGGTGTGCTCAGGATTGTGTGTTCGCTCCCTATTTTCCTGCGGATGAGCCCCAAAAGTTTGCTAATGTTCACAAAGTGTTTGGTGCCAGCAATGTGAACAAGATGTTGCAGGAGCTGCCAGAGCACCAGAGAGGAGATGCAGTTAGTAGCATGGTGTATGAAGCAAATGCCAGGGTGCGAGACCCAGTTTATGGGTGCGTTGGGGCAATTTCGTCTTTACAACAACAAATTGATGTGCTTCAAACCCAACTGGCTGTGGCTCAGGCAGAGGTGGTGCACCTCAAAGTGCGTCAGACCGCATCATTCTCGAACCATGGGTTAAGTCCGGCTAGCCCGAGTAATAGTAGGTCACCCTCATCTAAGTTCATGGGCTCTCAGGCTCGACCCATTTTCGACATGGATATGATGGTGGACCATACCAGCTTGGGAATGGGAGAGTCCATGTGGTCATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LOB

Protein Analysis

176

Amino Acids

19.09

Weight (kDa)

8.57

Isoelectric Point (pI)

64.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 15 - 112 6e-44 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 2 cut(s) 113, 369
AdeI CACNNNGTG 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 263
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 1 cut(s) 242
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
AluBI AGCT 3 cut(s) 61, 187, 495
AluI AGCT 3 cut(s) 61, 187, 495
Alw21I GWGCWC 4 cut(s) 37, 82, 198, 351
Alw26I GTCTC 1 cut(s) 246
Alw44I GTGCAC 2 cut(s) 33, 347
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 406
ApaLI GTGCAC 2 cut(s) 33, 347
ApeKI GCWGC 4 cut(s) 15, 18, 50, 187
AspS9I GGNCC 1 cut(s) 484
AsuHPI GGTGA 1 cut(s) 414
AsuNHI GCTAGC 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 1 cut(s) 484
BaeGI GKGCMC 2 cut(s) 37, 351
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 2 cut(s) 123, 446
Bbv12I GWGCWC 4 cut(s) 37, 82, 198, 351
BbvI GCAGC 4 cut(s) 27, 30, 62, 174
BccI CCATC 1 cut(s) 472
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 1 cut(s) 402
BisI GCNGC 4 cut(s) 16, 19, 51, 188
BlsI GCNGC 4 cut(s) 17, 20, 52, 189
Bme1390I CCNGG 1 cut(s) 244
Bme18I GGWCC 1 cut(s) 484
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 244
BmrI ACTGGG 1 cut(s) 251
BmsI GCATC 2 cut(s) 199, 380
BmtI GCTAGC 1 cut(s) 405
BmuI ACTGGG 1 cut(s) 251
Bpu10I CCTNAGC 2 cut(s) 81, 333
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 243, 385
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 263
Bse1I ACTGG 2 cut(s) 257, 327
Bse3DI GCAATG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 243, 385
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 263
BseMI GCAATG 1 cut(s) 169
BseMII CTCAG 4 cut(s) 79, 95, 347, 461
BseNI ACTGG 2 cut(s) 257, 327
BseRI GAGGAG 1 cut(s) 219
BseSI GKGCMC 2 cut(s) 37, 351
BseXI GCAGC 4 cut(s) 27, 30, 62, 174
BsgI GTGCAG 1 cut(s) 69
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 4 cut(s) 37, 82, 198, 351
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 1 cut(s) 398
BslI CCNNNNNNNGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 246
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 6 cut(s) 37, 82, 123, 198, 351, 446
Bsp19I CCATGG 1 cut(s) 385
BspACI CCGC 2 cut(s) 113, 369
BspCNI CTCAG 4 cut(s) 78, 94, 346, 460
BspLI GGNNCC 1 cut(s) 157
BspOI GCTAGC 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 169
BsrI ACTGG 2 cut(s) 257, 327
BssECI CCNNGG 2 cut(s) 243, 385
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 1 cut(s) 244
BstAPI GCANNNNNTGC 1 cut(s) 187
BstC8I GCNNGC 4 cut(s) 55, 59, 160, 403
BstDEI CTNAG 5 cut(s) 65, 81, 333, 432, 447
BstDSI CCRYGG 1 cut(s) 385
BstEII GGTNACC 1 cut(s) 420
BstF5I GGATG 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 187, 193
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 57
BstPI GGTNACC 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 242
BstSLI GKGCMC 2 cut(s) 37, 351
BstV1I GCAGC 4 cut(s) 27, 30, 62, 174
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 4 cut(s) 55, 59, 160, 403
CaiI CAGNNNCTG 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 484
CseI GACGC 1 cut(s) 350
CviAII CATG 7 cut(s) 54, 223, 386, 439, 469, 514, 522
DdeI CTNAG 5 cut(s) 65, 81, 333, 432, 447
DraIII CACNNNGTG 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 2 cut(s) 123, 446
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 484
Eco88I CYCGRG 1 cut(s) 406
Eco91I GGTNACC 1 cut(s) 420
EcoO65I GGTNACC 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 2 cut(s) 123, 446
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 7 cut(s) 57, 226, 389, 442, 472, 517, 525
FatI CATG 7 cut(s) 53, 222, 385, 438, 468, 513, 521
Fnu4HI GCNGC 4 cut(s) 16, 19, 51, 188
FokI GGATG 1 cut(s) 128
FriOI GRGCYC 2 cut(s) 123, 446
Fsp4HI GCNGC 4 cut(s) 16, 19, 51, 188
FspBI CTAG 1 cut(s) 402
GluI GCNGC 4 cut(s) 16, 19, 51, 188
HapII CCGG 1 cut(s) 398
HgaI GACGC 1 cut(s) 350
Hin1II CATG 7 cut(s) 57, 226, 389, 442, 472, 517, 525
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 1 cut(s) 509
HpaII CCGG 1 cut(s) 398
HphI GGTGA 1 cut(s) 414
Hpy166II GTNNAC 5 cut(s) 35, 142, 169, 349, 484
Hpy188I TCNGA 2 cut(s) 68, 366
Hpy188III TCNNGA 2 cut(s) 83, 379
Hpy8I GTNNAC 5 cut(s) 35, 142, 169, 349, 484
HpyAV CCTTC 1 cut(s) 66
HpyCH4V TGCA 6 cut(s) 35, 50, 57, 181, 212, 349
HpyF10VI GCNNNNNNNGC 2 cut(s) 187, 193
HpyF3I CTNAG 5 cut(s) 65, 81, 333, 432, 447
Hsp92II CATG 7 cut(s) 57, 226, 389, 442, 472, 517, 525
LmnI GCTCC 2 cut(s) 103, 184
Lsp1109I GCAGC 4 cut(s) 27, 30, 62, 174
LweI GCATC 2 cut(s) 199, 380
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 420
MboII GAAGA 1 cut(s) 81
MhlI GDGCHC 6 cut(s) 37, 82, 123, 198, 351, 446
MluCI AATT 2 cut(s) 279, 300
MlyI GAGTC 1 cut(s) 518
MnlI CCTC 5 cut(s) 49, 197, 334, 362, 436
MseI TTAA 1 cut(s) 392
MslI CAYNNNNRTG 1 cut(s) 473
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 1 cut(s) 244
MvaI CCWGG 1 cut(s) 244
MwoI GCNNNNNNNGC 2 cut(s) 187, 193
NcoI CCATGG 1 cut(s) 385
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 7 cut(s) 57, 226, 389, 442, 472, 517, 525
NlaIV GGNNCC 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 420
NspI RCATGY 1 cut(s) 57
PaeI GCATGC 1 cut(s) 57
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 4 cut(s) 17, 20, 52, 189
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
Psp6I CCWGG 1 cut(s) 242
PspEI GGTNACC 1 cut(s) 420
PspGI CCWGG 1 cut(s) 242
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 473
SaqAI TTAA 1 cut(s) 392
SatI GCNGC 4 cut(s) 16, 19, 51, 188
Sau96I GGNCC 1 cut(s) 484
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 1 cut(s) 244
SduI GDGCHC 6 cut(s) 37, 82, 123, 198, 351, 446
SetI ASST 8 cut(s) 34, 63, 77, 189, 345, 354, 422, 497
SfaNI GCATC 2 cut(s) 199, 380
SinI GGWCC 1 cut(s) 484
SmiMI CAYNNNNRTG 1 cut(s) 473
SphI GCATGC 1 cut(s) 57
Sse9I AATT 2 cut(s) 279, 300
SsiI CCGC 2 cut(s) 113, 369
SspMI CTAG 1 cut(s) 402
StyD4I CCNGG 1 cut(s) 242
StyI CCWWGG 1 cut(s) 385
TaqI TCGA 3 cut(s) 380, 454, 465
TasI AATT 2 cut(s) 279, 300
Tru1I TTAA 1 cut(s) 392
Tru9I TTAA 1 cut(s) 392
TseFI GTSAC 1 cut(s) 420
TseI GCWGC 4 cut(s) 15, 18, 50, 187
Tsp45I GTSAC 1 cut(s) 420
TspDTI ATGAA 3 cut(s) 17, 246, 427
Van91I CCANNNNNTGG 1 cut(s) 263
VneI GTGCAC 2 cut(s) 33, 347
VpaK11BI GGWCC 1 cut(s) 484
XceI RCATGY 1 cut(s) 57
XcmI CCANNNNNNNNNTGG 2 cut(s) 466, 499
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.