MD05G1225900.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010605
Physical Location & Seq
Forward (+)
35879718 .. 35880843
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1225900.v1.1.491

Sequence Viewer

Length: 528 bp
ATGGTGTTTAAAACGATCAATCTGAAAACAATATTTGCCCCTGCAAGTATTGGAGCGCTTGTTGGTTTTGCAATCGGAGTCATCCCTCAGATTCGAAAATTACTGATAGGAGATGGTGCTCCTCTACGGGTGATTCAAGATACTGCATATTTGTTGGGAGACGGAGCCATCCCAGCTCTCACTTTGATAATAGGAGGAAACCTACTTCAAGGTTTGAGAGGGTCAGGGATTCAGAAATCTCTTGTCGTTGGCATCATAGTTGTTCGATATGTTGCCCTGCCTCTTATAGGCGTCTTGGTTGTAAAAGGGGCACTGAAATTTGGGTTGGTGCACTCTGATCCATTATATCTGTTTGTTCTTCTGCTTCAGTTTTCACTCCCACCTGCGATGAACATAGGAATAATGACGCAGTTATTTGGAGCAGGAGAGAATGAATGTTCCGTTATCATGCTGTGGACTTATGCTTTCGCTTCACTGTCACTTACATTCTGGTCCGCCGCCTTCATGTGGCTTGTGGCGCGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0044425 GO:0051179 GO:0051234 GO:0055085
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mem_trans

Protein Analysis

176

Amino Acids

18.67

Weight (kDa)

9.66

Isoelectric Point (pI)

23.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mem_trans PF03547 4 - 164 7e-31 Membrane transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccII CGCG 1 cut(s) 520
AciI CCGC 2 cut(s) 495, 498
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 291
AfeI AGCGCT 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 287, 507
AgsI TTSAA 2 cut(s) 137, 209
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
Alw21I GWGCWC 2 cut(s) 121, 333
Alw26I GTCTC 1 cut(s) 153
Alw44I GTGCAC 1 cut(s) 329
AlwI GGATC 1 cut(s) 332
Aor51HI AGCGCT 1 cut(s) 57
ApaLI GTGCAC 1 cut(s) 329
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 2 cut(s) 58, 520
AspS9I GGNCC 1 cut(s) 492
AsuHPI GGTGA 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 492
BaeGI GKGCMC 2 cut(s) 313, 333
Bbv12I GWGCWC 2 cut(s) 121, 333
BccI CCATC 2 cut(s) 107, 176
BcoDI GTCTC 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
Bme18I GGWCC 1 cut(s) 492
BmgT120I GGNCC 1 cut(s) 492
BmiI GGNNCC 1 cut(s) 166
BmsI GCATC 1 cut(s) 261
Bpu14I TTCGAA 1 cut(s) 94
BsaHI GRCGYC 1 cut(s) 291
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 507
BseGI GGATG 2 cut(s) 81, 168
BseLI CCNNNNNNNGG 2 cut(s) 287, 507
BseMII CTCAG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 111
BseSI GKGCMC 2 cut(s) 313, 333
BseYI CCCAGC 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 520
BsiHKAI GWGCWC 2 cut(s) 121, 333
BslI CCNNNNNNNGG 2 cut(s) 287, 507
BsmAI GTCTC 1 cut(s) 153
BsmBI CGTCTC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 121, 313, 333
Bsp143I GATC 2 cut(s) 15, 337
BspACI CCGC 2 cut(s) 495, 498
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 1 cut(s) 332
BspT104I TTCGAA 1 cut(s) 94
BssMI GATC 2 cut(s) 15, 337
BssNI GRCGYC 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 477, 525
BstACI GRCGYC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 94
BstDEI CTNAG 1 cut(s) 87
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 81, 168
BstFNI CGCG 1 cut(s) 520
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 2 cut(s) 58, 520
BstKTI GATC 2 cut(s) 18, 340
BstMAI GTCTC 1 cut(s) 153
BstMBI GATC 2 cut(s) 15, 337
BstMWI GCNNNNNNNGC 2 cut(s) 173, 517
BstSLI GKGCMC 2 cut(s) 313, 333
BstUI CGCG 1 cut(s) 520
BtgZI GCGATG 1 cut(s) 401
BtsCI GGATG 2 cut(s) 81, 168
BtsIMutI CAGTG 2 cut(s) 311, 473
BveI ACCTGC 1 cut(s) 391
CfoI GCGC 2 cut(s) 58, 520
Cfr13I GGNCC 1 cut(s) 492
CseI GACGC 2 cut(s) 280, 415
CviAII CATG 2 cut(s) 448, 505
CviJI RGCY 3 cut(s) 167, 176, 511
CviKI_1 RGCY 3 cut(s) 167, 176, 511
DdeI CTNAG 1 cut(s) 87
DpnI GATC 2 cut(s) 17, 339
DpnII GATC 2 cut(s) 15, 337
DraI TTTAAA 1 cut(s) 10
EciI GGCGGA 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 492
Eco47III AGCGCT 1 cut(s) 57
Eco57I CTGAAG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 285
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 2 cut(s) 451, 508
FaiI YATR 9 cut(s) 148, 257, 270, 287, 346, 395, 449, 462, 506
FatI CATG 2 cut(s) 447, 504
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 2 cut(s) 68, 155
Fsp4HI GCNGC 1 cut(s) 498
GlaI GCGC 2 cut(s) 57, 519
GluI GCNGC 1 cut(s) 498
GsaI CCCAGC 1 cut(s) 176
HaeII RGCGCY 1 cut(s) 59
HgaI GACGC 2 cut(s) 280, 415
HhaI GCGC 2 cut(s) 58, 520
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 2 cut(s) 451, 508
Hin6I GCGC 2 cut(s) 56, 518
HinP1I GCGC 2 cut(s) 56, 518
HinfI GANTC 4 cut(s) 78, 91, 133, 229
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 331, 456
Hpy188I TCNGA 5 cut(s) 24, 77, 90, 234, 337
Hpy188III TCNNGA 1 cut(s) 137
Hpy8I GTNNAC 2 cut(s) 331, 456
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 2 cut(s) 477, 525
HpyCH4V TGCA 4 cut(s) 44, 71, 146, 331
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 517
HpyF3I CTNAG 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 2 cut(s) 451, 508
HspAI GCGC 2 cut(s) 56, 518
Kzo9I GATC 2 cut(s) 15, 337
LmnI GCTCC 4 cut(s) 53, 124, 164, 419
LpnPI CCDG 7 cut(s) 54, 186, 210, 290, 396, 408, 475
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 477
MalI GATC 2 cut(s) 17, 339
MboI GATC 2 cut(s) 15, 337
MboII GAAGA 1 cut(s) 350
MhlI GDGCHC 3 cut(s) 121, 313, 333
MluCI AATT 2 cut(s) 98, 317
MlyI GAGTC 1 cut(s) 87
MnlI CCTC 5 cut(s) 96, 132, 188, 212, 291
MseI TTAA 1 cut(s) 9
MvnI CGCG 1 cut(s) 520
MwoI GCNNNNNNNGC 2 cut(s) 173, 517
NdeII GATC 2 cut(s) 15, 337
NlaIII CATG 2 cut(s) 451, 508
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 477
NspV TTCGAA 1 cut(s) 94
PaqCI CACCTGC 1 cut(s) 391
PfeI GAWTC 3 cut(s) 91, 133, 229
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PspFI CCCAGC 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 492
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 2 cut(s) 15, 337
Sau96I GGNCC 1 cut(s) 492
SchI GAGTC 1 cut(s) 87
SduI GDGCHC 3 cut(s) 121, 313, 333
SetI ASST 4 cut(s) 178, 204, 214, 385
SfaNI GCATC 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 94
SinI GGWCC 1 cut(s) 492
Sse9I AATT 2 cut(s) 98, 317
SsiI CCGC 2 cut(s) 495, 498
SspI AATATT 1 cut(s) 33
TaaI ACNGT 2 cut(s) 477, 525
TaqI TCGA 2 cut(s) 94, 265
TasI AATT 2 cut(s) 98, 317
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 3 cut(s) 91, 133, 229
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 2 cut(s) 318, 480
TseFI GTSAC 1 cut(s) 477
Tsp45I GTSAC 1 cut(s) 477
TspDTI ATGAA 3 cut(s) 404, 447, 493
TspGWI ACGGA 2 cut(s) 177, 430
TspRI CASTG 2 cut(s) 318, 480
VneI GTGCAC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 492
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.